CN109725098A - A kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method - Google Patents
A kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method Download PDFInfo
- Publication number
- CN109725098A CN109725098A CN201910165684.3A CN201910165684A CN109725098A CN 109725098 A CN109725098 A CN 109725098A CN 201910165684 A CN201910165684 A CN 201910165684A CN 109725098 A CN109725098 A CN 109725098A
- Authority
- CN
- China
- Prior art keywords
- rhynchophyllin
- cigarette
- rate
- flue gas
- filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method, by being balanced processing to the cigarette containing rhynchophyllin feed liquid, then respectively to pipe tobacco and filter disc, cigarette holder carries out that extract liquor is obtained by extraction, using the rhynchophyllin in gas chromatography mass spectrometry choice ion pattern measurement cigarette smoke, improve the sensitivity and accuracy of detection, the area ratio of rhynchophyllin calculates the rate of transform and rejection of the rhynchophyllin in cigarette smoke in cigarette shreds shared by area by rhynchophyllin in filter disc and filter tip, important reference frame is provided for effect research of the new type natural tobacco aromaticss in cigarette, research and development simultaneously for functional form or drug cigarette are of great significance, the present invention is added in pipe tobacco from rhynchophyllin, it is transferred to cigarette smoke after to cigarette burning and neutralizes the amount being trapped in cigarette filter, make rhynchophyllin in cigarette Effect in sensory evaluating smoking obtains specific quantitatively evaluating, while providing for the quantitatively evaluating of other function cigarette a kind of with reference to method.
Description
Technical field
The invention belongs to chemical analysis detection technique fields, and in particular to a kind of flue gas rhynchophyllin rate of transform and filter tip uncaria
Alkali rejection measuring method.
Background technique
Rhynchophyllin is the extracted alkaloid of buckle branch of madder wort uncaria, is had for traditional Chinese medicine Uncaria genus plant
One of the main component for imitating ingredient has cardiovascular system of human body, central nervous system and hematological system etc. specific raw
Object active function is the common medicine source for treating hypertension prescription.In recent years, with the nutritive value of uncaria, medicinal function by
Gradually recognized by people, using relatively more in terms of Chinese medicine, but it is less to compare in terms of the research of cigarette, opens third of the twelve Earthly Branches etc. within 2011 years
New natural tobacco Flavour resources are artificially developed, " constituent analysis of uncaria sonicated extract and the application in cigarette " is had studied,
And the research about the rhynchophyllin rate of transform in flue gas rarely has people's report.
Summary of the invention
The purpose of the present invention is to provide a kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method, with
Overcome the deficiencies in the prior art.
In order to achieve the above objectives, the present invention adopts the following technical scheme:
A kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method, comprising the following steps:
Step 1) will add the cigarette containing rhynchophyllin feed liquid at 21-23 DEG C, and RH is balanced at least in 58-62% environment
48 hours, then it is divided into two groups；
Step 2) separates the pipe tobacco in cigarette to first group of cigarette after Balance Treatment, then to the pipe tobacco separated
Filtrate is removed after first extracting alkane derivative with petroleum ether, it is molten that ammonium hydroxide then is added to the pipe tobacco after extraction alkane derivative
Liquid complete wetting pipe tobacco adds dichloromethane solution concussion extraction pipe tobacco not less than 1 hour and obtains extract liquor, to extract liquor into
Row GC/MS analysis, obtains the area of rhynchophyllin in cigarette shreds；
Step 3) aspirates second group of cigarette after Balance Treatment, using flue gas trapping filter disc to drawing smoke mistake
Filter, then by the flue gas trapping filter disc after cigarette filter and filtering flue gas, concussion extraction obtains at least 1 hour in methyl alcohol respectively
Then extract liquor carries out GC/MS analysis to extract liquor respectively, respectively obtain in filter tip rhynchophyllin in the area and filter disc of rhynchophyllin
Area；
Step 4), in flue gas the rhynchophyllin rate of transform use calculation of area, i.e., the transfer of rhynchophyllin in cigarette smoke
Rate is equal to the area of rhynchophyllin in filter disc divided by the area of rhynchophyllin in cigarette shreds, the rate of transform etc. of rhynchophyllin in cigarette filter
In filter tip the area of rhynchophyllin divided by the rhynchophyllin in cigarette shreds area.
Further, use ammonia spirit concentration for 5-10%.
Further, GC/MS analyzes detailed process are as follows: sample to be tested is through 7890B gas chromatograph autosampler sample introduction
Afterwards, the transient evaporation under 280 DEG C of injector temperature, then brings gasifying gas into gas chromatography-mass spectrography by carrier gas
Instrument carries out separation detection using the capillary chromatographic column in gas chromatograph-mass spectrometer (GC-MS), rhynchophyllin component content can be obtained
As a result.
Further, gas chromatograph-mass spectrometer (GC-MS) (GC/MS) uses 7890B-5977A gas chromatography-mass spectrography
Instrument.
Further, capillary chromatographic column uses HP-5MS capillary column.
Further, use GC/MS analyze in carrier gas for He, carrier gas flux: 1.2mL/min.
Further, the initial temperature of gas chromatograph column oven is risen to since 80 DEG C with 5 DEG C/min heating rate
290 DEG C, 30min is kept at 290 DEG C.
Further, using GC/MS analysis condition are as follows: transmission line temperature: 280 DEG C；Ionization mode: the source EI；Ionizing energy:
70eV；Ion source temperature: 230 DEG C；Level four bars temperature: 150 DEG C；Solvent delay: 3.5min；Scanning range: 35~500amu；Inspection
Survey mode: full scan mode is qualitative, and SIM mode is quantitative；The characteristic ion (m/z) of rhynchophyllin: 384.
Compared with prior art, the invention has the following beneficial technical effects:
A kind of flue gas rhynchophyllin rate of transform of this method and filter tip rhynchophyllin rejection measuring method, by containing rhynchophyllin
The cigarette of feed liquid is balanced processing, then carries out that extract liquor is obtained by extraction to pipe tobacco and filter disc, cigarette holder respectively, is added a certain amount of
Dilute alkaline soln, rhynchophyllin separate out can be made, drifting alkaloids are easier to extract than reference state alkaloid, improve the effect of extraction
Fruit, then using gas chromatography mass spectrometry choice ion pattern measurement extract liquor in rhynchophyllin area, improve detection sensitivity and
Accuracy, the area ratio of rhynchophyllin calculates rhynchophyllin and is rolling up in cigarette shreds shared by the area by rhynchophyllin in filter disc and filter tip
The rate of transform and rejection in cigarette flue gas, algorithm is simple, and accuracy is high, for the effect of new type natural tobacco aromaticss in cigarette
Research provides important reference frame, and the research and development simultaneously for functional form or drug cigarette are of great significance, and the present invention is from uncaria
Alkali is added in pipe tobacco, to cigarette burning after be transferred to cigarette smoke and neutralize the amount that is trapped in cigarette filter, so that rhynchophyllin is existed
Effect in cigarette sensory evaluating smoking obtains specific quantitatively evaluating, while the quantitatively evaluating for other function cigarette provides one kind
With reference to method.
Detailed description of the invention
Fig. 1 is the selection ion scan SIM figure of rhynchophyllin in cigarette shreds.
Fig. 2 is the SIM figure that cigarette smoke traps rhynchophyllin in filter disc and cigarette filter.
Fig. 3 is the SIM figure of rhynchophyllin in embodiment different number cigarette.
Specific embodiment
The invention will be described in further detail with reference to the accompanying drawing:
The purpose of the present invention is to provide a kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method, adopt
With gas chromatography mass spectrometry, selects ion scan detection method to improve the detection sensitivity and accuracy of rhynchophyllin, make the transfer of rhynchophyllin
The calculating of rate and rejection is easier, and rhynchophyllin is made to obtain quantitatively evaluating in cigarette smoke.
A kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method, comprising the following steps:
Step 1) will add the cigarette containing rhynchophyllin feed liquid at 21-23 DEG C, and RH is balanced at least in 58-62% environment
48 hours, two groups are then divided into, keeps the water tariff collection of cigarette consistent；
Step 2) separates the pipe tobacco in cigarette to first group of cigarette after Balance Treatment, then to the pipe tobacco separated
After first extracting alkane derivative with petroleum ether, filtrate is removed, ammonia spirit complete wetting pipe tobacco is then added, then use dichloromethane
Alkane solution concussion extraction pipe tobacco is not less than 1 hour, carries out GC/MS analysis to extract liquor, analyzes the amount of rhynchophyllin in cigarette shreds,
It obtains；
Use ammonia spirit concentration for 5-10%；
Step 3) aspirates second group of cigarette after Balance Treatment, using flue gas trapping filter disc to drawing smoke mistake
Filter, then by filter flue gas after flue gas trapping filter disc and cigarette filter respectively in methyl alcohol concussion extraction at least 1 hour, then
GC/MS analysis is carried out to extract liquor respectively, respectively obtains the area of rhynchophyllin in the area and filter tip of rhynchophyllin in filter disc, is detected
The rate of transform of rhynchophyllin in cigarette smoke condensates；
Step 4), in flue gas the rhynchophyllin rate of transform use calculation of area, i.e., the transfer of rhynchophyllin in cigarette smoke
Rate is equal to the area of rhynchophyllin in filter disc divided by the area of rhynchophyllin in cigarette shreds, the rate of transform etc. of rhynchophyllin in cigarette filter
In filter tip the area of rhynchophyllin divided by the rhynchophyllin in cigarette shreds area.
GC/MS analyzes detailed process are as follows: sample to be tested is after 7890B gas chromatograph autosampler sample introduction, at 280 DEG C
Injector temperature under transient evaporation, gas chromatograph-mass spectrometer (GC-MS) then into is brought gasifying gas by carrier gas, utilizes gas phase
Capillary chromatographic column in chromatograph-mas spectrometer carries out separation detection, and rhynchophyllin component content result can be obtained.
Gas chromatograph-mass spectrometer (GC-MS) (GC/MS) uses 7890B-5977A gas chromatograph-mass spectrometer (GC-MS)；
Capillary chromatographic column uses HP-5MS capillary column；
Using GC/MS analysis condition: chromatographic column: HP-5MS capillary column (30m × 0.25mm × 0.25 μm)；Carrier gas: He；
Carrier gas flux: 1.2mL/min；Injector temperature: 280 DEG C；Split ratio: 10:1；Sample volume: 1 μ L；Temperature programming: initial temperature
290 DEG C are risen to from 80 with 5 DEG C/min heating rate, keeps 30min at 290 DEG C；Transmission line temperature: 280 DEG C；Ionization mode:
The source EI；Ionizing energy: 70eV；Ion source temperature: 230 DEG C；Level four bars temperature: 150 DEG C；Solvent delay: 3.5min；Scan model
It encloses: 35~500amu；Detection mode: full scan mode is qualitative, and SIM mode is quantitative；The characteristic ion (m/z) of rhynchophyllin: 384.
Experiment chooses two kinds of methanol, methylene chloride organic solvents respectively to cigarette shreds, flue gas trapping filter disc, cigarette filter
In rhynchophyllin extracted, the experimental results showed that, methylene chloride when extracting filter tip, filter tip dissolution, be unsuitable for hook in filter tip
The extraction of rattan alkali, therefore final choice cigarette shreds are extracted with dichloromethane, filter disc and filter tip methanol extraction.
Experiment is compared two kinds of extraction modes of ultrasonic extraction and oscillation extraction, the results showed that, ultrasonic extraction process water
Temperature can be increased with extraction time, and impact analysis is as a result, event slective extraction mode is oscillation extraction, so as to guarantee test condition
Consistency.
Rhynchophyllin is a kind of vegetable soda, in order to make the better separate out of rhynchophyllin, test rhynchophyllin in cigarette shreds
Extraction process in, done the test of a certain amount of 5% ammonium hydroxide of addition with being not added compared with ammonium hydroxide tests, the results showed that, do not ammoniate
Rhynchophyllin is not detected in the cigarette shreds extract liquor of water, and is added in the cigarette shreds extract liquor of a certain amount of 5% ammonium hydroxide and is detected
Rhynchophyllin out, therefore suitable 5% ammonium hydroxide is added in the processing selection of cigarette shreds, rhynchophyllin belongs to alkaloid, is added a certain amount of
Dilute alkaline soln, rhynchophyllin separate out can be made, drifting alkaloids are easier to extract than reference state alkaloid, improve the effect of extraction
Fruit；
In specific implementation process: methanol concussion extraction 1 hour of the cigarette smoke aspirated trapping filter disc 50mL extracts
GC/MS analysis is carried out after taking liquid to be concentrated to 0.5mL；The cigarette filter aspirated is added methanol concussion extraction 1 hour of 50mL,
Extract liquor carries out GC/MS analysis after being concentrated to 0.5mL；
Containing for rhynchophyllin in the cigarette shreds, flue gas trapping filter disc and cigarette filter of 5,10,20 cigarette has been investigated in experiment
Measurement is pledged love condition, and test result shows that the rhynchophyllin effect of extracting of 20 cigarette is relatively good, as shown in Figure 1 to Figure 3.
The effect of extracting of rhynchophyllin compares in 1 different number Cigarette of table, and area refers to rhynchophyllin characteristic ion 384
The area at peak:
The calculating of the rhynchophyllin rate of transform
It tests and calculation of area is used to the rhynchophyllin rate of transform, i.e., the rate of transform of rhynchophyllin is equal to filter disc in cigarette smoke
The area of middle rhynchophyllin is divided by the area of rhynchophyllin in cigarette shreds, and the rate of transform of rhynchophyllin is equal to hook in filter tip in cigarette filter
The area of rattan alkali divided by the rhynchophyllin in cigarette shreds area.
In flue gas in the rate of transform=filter disc of rhynchophyllin in area/cigarette shreds of rhynchophyllin rhynchophyllin area
In filter tip in rejection=filter tip of rhynchophyllin in area/cigarette shreds of rhynchophyllin rhynchophyllin area
The measurement result of the rhynchophyllin rate of transform and rejection in cigarette smoke
The rate of transform and rejection measurement result of rhynchophyllin in 2 cigarette smoke of table
| The middle area of pipe tobacco | Area in filter disc | Area in filter tip | The rate of transform (%) | Rejection (%) |
| 187941 | 11507 | 10200 | 6.12 | 5.43 |
This method passes through to rhynchophyllin in rhynchophyllin in rhynchophyllin in cigarette shreds, flue gas trapping filter disc, cigarette filter
Measurement calculates the rate of transform and rejection of the rhynchophyllin in cigarette smoke using calculation of area, and this method has certain
Novelty provides important reference frame to effect research of the new type natural tobacco aromaticss in cigarette, simultaneously for functional form
Or the research and development of drug cigarette are of great significance.
Claims (8)
1. a kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method, which comprises the following steps:
Step 1) will add the cigarette containing rhynchophyllin feed liquid at 21-23 DEG C, and it is small that RH balances at least 48 in 58-62% environment
When, then it is divided into two groups；
Step 2) separates the pipe tobacco in cigarette to first group of cigarette after Balance Treatment, then first uses the pipe tobacco separated
Petroleum ether removes filtrate after extracting alkane derivative, and it is complete that ammonia spirit then is added to the pipe tobacco after extraction alkane derivative
Full infiltration pipe tobacco adds dichloromethane solution concussion extraction pipe tobacco not less than 1 hour and obtains extract liquor, carries out to extract liquor
GC/MS analysis, obtains the area of rhynchophyllin in cigarette shreds；
Step 3) aspirates second group of cigarette after Balance Treatment, is filtered using flue gas trapping filter disc to drawing smoke, so
By the flue gas trapping filter disc after cigarette filter and filtering flue gas, concussion extraction is extracted at least 1 hour in methyl alcohol respectively afterwards
Then liquid carries out GC/MS analysis to extract liquor respectively, respectively obtain the face of rhynchophyllin in the area and filter disc of rhynchophyllin in filter tip
Product；
Step 4), in flue gas the rhynchophyllin rate of transform use calculation of area, i.e., the rate of transform etc. of rhynchophyllin in cigarette smoke
The area of rhynchophyllin is divided by the area of rhynchophyllin in cigarette shreds in filter disc, and the rate of transform of rhynchophyllin is equal to filter in cigarette filter
In mouth the area of rhynchophyllin divided by the rhynchophyllin in cigarette shreds area.
2. a kind of flue gas rhynchophyllin rate of transform according to claim 1 and filter tip rhynchophyllin rejection measuring method, special
Sign is, uses ammonia spirit concentration for 5-10%.
3. a kind of flue gas rhynchophyllin rate of transform according to claim 1 and filter tip rhynchophyllin rejection measuring method, special
Sign is that GC/MS analyzes detailed process are as follows: sample to be tested is after 7890B gas chromatograph autosampler sample introduction, at 280 DEG C
Injector temperature under transient evaporation, gas chromatograph-mass spectrometer (GC-MS) then into is brought gasifying gas by carrier gas, utilizes gas phase
Capillary chromatographic column in chromatograph-mas spectrometer carries out separation detection, and rhynchophyllin component content result can be obtained.
4. a kind of flue gas rhynchophyllin rate of transform according to claim 3 and filter tip rhynchophyllin rejection measuring method, special
Sign is that gas chromatograph-mass spectrometer (GC-MS) uses 7890B-5977A gas chromatograph-mass spectrometer (GC-MS).
5. a kind of flue gas rhynchophyllin rate of transform according to claim 3 and filter tip rhynchophyllin rejection measuring method, special
Sign is that capillary chromatographic column uses HP-5MS capillary column.
6. a kind of flue gas rhynchophyllin rate of transform according to claim 3 and filter tip rhynchophyllin rejection measuring method, special
Sign is, use GC/MS analyze in carrier gas for He, carrier gas flux: 1.2mL/min.
7. a kind of flue gas rhynchophyllin rate of transform according to claim 3 and filter tip rhynchophyllin rejection measuring method, special
Sign is that the initial temperature of gas chromatograph column oven rises to 290 DEG C since 80 DEG C with 5 DEG C/min heating rate, at 290 DEG C
Lower holding 30min.
8. a kind of flue gas rhynchophyllin rate of transform according to claim 3 and filter tip rhynchophyllin rejection measuring method, special
Sign is, using GC/MS analysis condition are as follows: transmission line temperature: 280 DEG C；Ionization mode: the source EI；Ionizing energy: 70eV；Ion
Source temperature: 230 DEG C；Level four bars temperature: 150 DEG C；Solvent delay: 3.5min；Scanning range: 35~500amu；The spy of rhynchophyllin
Levy ion m/z:384.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910165684.3A CN109725098B (en) | 2019-03-04 | 2019-03-04 | Method for measuring rhynchophylline transfer rate and rhynchophylline retention rate of filter tip of smoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910165684.3A CN109725098B (en) | 2019-03-04 | 2019-03-04 | Method for measuring rhynchophylline transfer rate and rhynchophylline retention rate of filter tip of smoke |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109725098A true CN109725098A (en) | 2019-05-07 |
| CN109725098B CN109725098B (en) | 2022-03-11 |
Family
ID=66301736
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910165684.3A Active CN109725098B (en) | 2019-03-04 | 2019-03-04 | Method for measuring rhynchophylline transfer rate and rhynchophylline retention rate of filter tip of smoke |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109725098B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1572317A (en) * | 2003-06-03 | 2005-02-02 | 霍玉书 | Anti-allergic traditional Chinese medicine composition and preparing method thereof |
| CN103869010A (en) * | 2014-02-25 | 2014-06-18 | 广东药学院 | HPLC (High performance liquid chromatography) detection method for distinguishing different cultivars of uncaria |
| CN105301136A (en) * | 2015-11-23 | 2016-02-03 | 江苏康缘药业股份有限公司 | Nine-element wind-extinguishing particle component quantitative detection method and fingerprint construction method |
| CN105699305A (en) * | 2016-01-29 | 2016-06-22 | 陕西中烟工业有限责任公司 | Method for measuring transfer rate of cigarette saponins additive in main stream smoke |
| CN105699519A (en) * | 2016-01-29 | 2016-06-22 | 云南同创检测技术股份有限公司 | Analysis method of transferring rate of nicotine in smoke in electronic cigarette smoking process |
| CN105784912A (en) * | 2016-05-20 | 2016-07-20 | 江苏中烟工业有限责任公司 | Method for quantitatively analyzing lactones aromatic substances in mainstream smoke of fine cigarettes |
| CN106908546A (en) * | 2016-11-13 | 2017-06-30 | 云南中烟工业有限责任公司 | The gas-chromatography tandem mass spectrum detection method of bactericide migration amount in cigarette mainstream flue gas |
| CN107490642A (en) * | 2017-08-23 | 2017-12-19 | 国家烟草质量监督检验中心 | Method that is a kind of while determining 12 kinds of alkaloids in cigarette smoke granule phase substance |
| CN108627581A (en) * | 2018-03-01 | 2018-10-09 | 广州王老吉药业股份有限公司 | Rhynchophyllin and isorhynchophylline content assaying method in a kind of Xiao ' erqixingcha particle |
-
2019
- 2019-03-04 CN CN201910165684.3A patent/CN109725098B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1572317A (en) * | 2003-06-03 | 2005-02-02 | 霍玉书 | Anti-allergic traditional Chinese medicine composition and preparing method thereof |
| CN103869010A (en) * | 2014-02-25 | 2014-06-18 | 广东药学院 | HPLC (High performance liquid chromatography) detection method for distinguishing different cultivars of uncaria |
| CN105301136A (en) * | 2015-11-23 | 2016-02-03 | 江苏康缘药业股份有限公司 | Nine-element wind-extinguishing particle component quantitative detection method and fingerprint construction method |
| CN105699305A (en) * | 2016-01-29 | 2016-06-22 | 陕西中烟工业有限责任公司 | Method for measuring transfer rate of cigarette saponins additive in main stream smoke |
| CN105699519A (en) * | 2016-01-29 | 2016-06-22 | 云南同创检测技术股份有限公司 | Analysis method of transferring rate of nicotine in smoke in electronic cigarette smoking process |
| CN105784912A (en) * | 2016-05-20 | 2016-07-20 | 江苏中烟工业有限责任公司 | Method for quantitatively analyzing lactones aromatic substances in mainstream smoke of fine cigarettes |
| CN106908546A (en) * | 2016-11-13 | 2017-06-30 | 云南中烟工业有限责任公司 | The gas-chromatography tandem mass spectrum detection method of bactericide migration amount in cigarette mainstream flue gas |
| CN107490642A (en) * | 2017-08-23 | 2017-12-19 | 国家烟草质量监督检验中心 | Method that is a kind of while determining 12 kinds of alkaloids in cigarette smoke granule phase substance |
| CN108627581A (en) * | 2018-03-01 | 2018-10-09 | 广州王老吉药业股份有限公司 | Rhynchophyllin and isorhynchophylline content assaying method in a kind of Xiao ' erqixingcha particle |
Non-Patent Citations (2)
| Title |
|---|
| 高广涛等: "气相色谱（GC）法测定钩藤中钩藤碱的含量", 《湖北农业科学》 * |
| 高志娟: "低危害卷烟中中草药有效成分分析及其降害作用研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109725098B (en) | 2022-03-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104820040B (en) | Method for measuring nicotine content in gum base type chewing tobacco | |
| CN104655778B (en) | A kind of method for measuring 18 kinds of volatilizations and semi-volatile organic matter content in tobacco juice for electronic smoke and aerosol | |
| CN103105437B (en) | Method for simultaneous determination of residual quantity of 8 ester allergenic aromatics in toys | |
| CN104237420A (en) | Method for building cigarette bitter taste index evaluation model based on taste activity value | |
| CN106324130A (en) | Chiral analysis method for nicotine in cigarette cut tobacco | |
| CN106706794A (en) | Quantitative analysis method of main moss fragrance characteristics flavor component in fine cigarette mainstream smoke | |
| CN110412183B (en) | Rose fragrance component analysis method by needle capture-gas chromatography-mass spectrometry | |
| CN105784912A (en) | Method for quantitatively analyzing lactones aromatic substances in mainstream smoke of fine cigarettes | |
| CN105675757A (en) | Method for determining tobacco-specific N-nitrosamine and polycyclic aromatic hydrocarbons in mainstream smoke of cigarettes simultaneously | |
| CN103257194A (en) | GC-MS/MS (Gas chromatography - tandem mass spectrometry) method for simultaneously analyzing three polycyclic aromatic hydrocarbons and four tobacco-specific nitrosamines in main stream smoke of cigarettes | |
| CN105527356B (en) | The peculiar N nitrosamine of tobacco and assay method while polycyclic aromatic hydrocarbon in a kind of cigarette mainstream flue gas based on suction nozzle micro-extraction | |
| CN106749143A (en) | A kind of method that scopolactone compound is extracted from tobacco | |
| CN108548877A (en) | The method that solid-phase microextraction technology measures the quick-fried pearl volatile ingredient of cigarette | |
| CN103558319B (en) | Method for measuring methyl eugenol in mainstream smoke of cigarettes | |
| CN108548880B (en) | Method for measuring various components in electronic cigarette smoke | |
| CN106018658A (en) | Multidimensional gas chromatography-tandem mass spectrometry analytical method for nicotine optical isomer in electronic cigarette smoke solution | |
| CN102967670A (en) | Method for measuring cordycepin, adenosine and mannitol in cordyceps sinensis mycelium powder | |
| CN103257195B (en) | GC-MS/MS (Gas chromatography-tandem mass spectrometry) method for simultaneously analyzing benzo [a] BaA, chrysene and benzo [a] BaP in main stream smoke of cigarettes | |
| CN103713058B (en) | The detection method of diester-type alkaloids in a kind of Huafeng pill medicine mother | |
| CN109725098A (en) | A kind of flue gas rhynchophyllin rate of transform and filter tip rhynchophyllin rejection measuring method | |
| CN115950979B (en) | Method for tracing origin of complex matrix tobacco extract | |
| CN101703610A (en) | Quality detection method of Qingnao antihypertensive tablet | |
| CN107064364A (en) | The assay method of Performance Liquid Chromatography Analysis for Nicotine in Tobacco optical isomer | |
| CN114216980B (en) | Method for establishing HPLC-ELSD fingerprint of starwort root | |
| CN103558312B (en) | Method for measuring benzo[a]pyrene content of mainstream smoke of cigarettes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |