CN109719070A - A kind of device of the incubation carrying out washing treatment for biological sample and its incubation, recycling antibody method - Google Patents
A kind of device of the incubation carrying out washing treatment for biological sample and its incubation, recycling antibody method Download PDFInfo
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- CN109719070A CN109719070A CN201910029780.5A CN201910029780A CN109719070A CN 109719070 A CN109719070 A CN 109719070A CN 201910029780 A CN201910029780 A CN 201910029780A CN 109719070 A CN109719070 A CN 109719070A
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Abstract
The invention discloses a kind of devices of incubation carrying out washing treatment for biological sample, it further include that the supporting frame of groove profile and the liquid arranged from bottom to up in the supporting frame of groove profile collect accumulator tank, the combination of swing type drum wash, the combination of rotary liquid container including motor and control device；The rotary liquid container group be combined into upper end it is open and can external driver device drive under rotate pouring liquid container；The swing type drum wash combination includes for being incubated for the drum apparatus washed to biological sample and liquid in the combination of rotary liquid container being imported to the washing trough upper ledge of drum apparatus；The liquid collects the bottom that accumulator tank is located at supporting frame；In view of the diversity with liquid, and devise multiple solution tanks；Antibody-solutions recoverable, save the cost；It is abundant to be incubated for washing；Barrel mast formula, which squeezes, increases liquid structure, can be further reduced antibody-solutions requirement.
Description
Technical field
The present invention relates to the process field of biological sample film or glue, specifically a kind of incubation for ProBloot membrane
The dyeing of gel after carrying out washing treatment or protein electrophoresis, the device of decolorization and and its incubation, recycling antibody method
Background technique
The method of western blot detection, has come out more than 40 years.The incubation of ProBloot membrane is washed, and is much being tested
Room remains the method for being incubated for washing using liquid is manually changed.Have on the market and much can be carried out automatic incubation washing apparatus equipment
For everybody selection, wherein most of machines are rocked using horizontal shaker, liquid pump to supply refrigerant realizes the function of antibody incubation washing.
Since this kind of machine system is complicated, it is at high price, and antibody consumption amount is high the disadvantages of presence, and cannot be used by laboratory.
The present invention devises a kind of device of simple motor control machinery, the overall process for being incubated for washing to complete ProBloot membrane.
Device system is simple, moderate, and antibody consumption amount is small, and antibody, no cross contamination can be recycled.
Summary of the invention
The present invention is multiple in order to solve the incubation wash mill machine system of the ProBloot membrane in currently available technology
It is miscellaneous, it is at high price, it is incubated for the problem that washing is insufficient and antibody consumption amount is high.
The technical solution adopted by the present invention is that: a kind of device of the incubation carrying out washing treatment for biological sample, including groove profile
Supporting frame and the liquid arranged from bottom to up in the supporting frame of groove profile collect accumulator tank, swing type drum wash group
Conjunction, the combination of rotary liquid container, motor and control device；It is open that the rotary liquid container group is combined into upper end
And the container of pouring liquid can be rotated under external driver device drive；The swing type drum wash combination includes for life
The drum apparatus and import liquid in the combination of rotary liquid container in the washing trough of drum apparatus that object sample incubation washs
Frame；The liquid collects the bottom that accumulator tank is located at supporting frame, the liquid poured out for collection cylinder device.
Further, the biological sample is membranaceous biological sample such as ProBloot membrane, polyacrylamide gel.
Further, the rotary liquid container combination is made of the superposition of multiple liquid container monomers；Liquid
Body storage container monomer include upper end opening the chamber for storing liquid, through in the middle part of chamber axis hole, by external drive
The chamber driving gear of device driving and liner sample container that be preinstalled with solution and that middle part is slotted；Under the chamber that liquid can be stored
Portion is in toothed arc, and chamber driving gear is meshed with chamber lower portion and can drive chamber under the driving of external driver device
There are the flow-guiding mouth for facilitating liquid to flow out in rotation, the side of chamber upper opening；Liner sample container and the chamber that liquid can be stored
Inner wall shape, which matches to merge, to be placed among liquid container.
Further, the side view of each liquid container monomer is identical, but can have different thickness；It is each
The driving gear of external container, it is staggered；Corresponding liquid container is respectively driven, its opening can be made to fluid storage
The extreme lower position of container monomer, allows liquid to flow out.
Further, the device according to claim for the incubation carrying out washing treatment of biological sample, feature exist
In: the upper end of the washing trough upper ledge is liquid collection trough, and one end of liquid collection trough is equipped with the V-type liquid communicated with liquid collection trough
Circulation road under body, the lower end of lower circulation road are equipped with the liquid flowing outlet communicated with drum apparatus；The other end of liquid collection trough is set
There is V-type fixture block, there is washing basket lower frame plug-in mounting short axle in its underpart；One is respectively provided on two opposite medial surfaces of supporting frame
V-type strip protrusion, liquid collection trough and fixture block can be caught in the V-type strip protrusion on respective side, and the drum apparatus includes
Tubular washing basket, washing basket external drive gear and washing trough lower frame；Washing trough lower frame includes being located at the fluted disc of washing trough lower frame two sides and being used for
The cambered surface of connecting chainring, the medial surface of fluted disc are smooth plane；There are a gear teeth at the lateral surface edge of fluted disc, the gear teeth can with outside washing basket
Portion drives gear engagement；The center of two sides fluted disc is equipped with aperture, for fixed short axle and liquid flowing outlet insertion；Washing trough lower frame
Lower wall is the cambered surface for placement, and tubular washing basket is installed in cambered surface, and cambered surface is connected with left and right fluted disc, forms washing incubation slot simultaneously
Drive tubular washing basket rotation；The both side edges of cambered surface have liquid guide flow mouth；
Further, fixture block lateral wall is equipped with positioning jump ring slot, is equipped with Clip Reed in positioning jump ring slot；Under Clip Reed
Portion is in for shape protrusion, and the top of Clip Reed is in inverted L shape, where the upper end of Clip Reed can be caught in V-type fixture block on the fluted disc of side
Bayonet in；When Clip Reed is located in jump-ring slot, bayonet is inserted into the upper end of Clip Reed, locks the position of washing trough lower frame；Work as swing
When formula roller washes film combination insertion Film washer supporting frame, when Clip Reed lower part is pressurized for shape protrusion, the upper end abjection of Clip Reed
Bayonet, the swing that at this moment washing trough lower frame can be unrestricted.
Further, the tubular washing basket is the tubular structure that diaphragm is rolled into, and is equipped with barrel mast formula in tubular washing basket and squeezes
Increase liquid structure, has a shaped opening on diaphragm, it can be by the inside of the angularly disposed No.1 moving tooth in liquid collection trough lower end surface and cambered surface
The direction of No. two angularly disposed moving teeth insertion on face, No.1 moving tooth and No. two moving teeth is all clockwise, or is all counterclockwise.
Further, when washing trough upper ledge is fixed, and washing trough lower frame is driven by washing basket external drive gear, with central openings
For axle center, the limited rotary oscillation of both forward and reverse directions, so that tubular washing basket stirs rotation by No.1 moving tooth and No. two moving teeth in succession；It washes
It washs as follows with biological sample details in incubation slot: being incubated for washing ProBloot membrane, be placed the medial surface for being attached to washing basket, barrel mast
Formula squeezes increasing liquid structure and is placed in washing basket；When having liquid in washing trough, liquid can collect in the bottom of washing trough.Western blot
Film can be attached to washing basket inner wall, and its underpart can be immersed among liquid；Barrel mast formula extruding increasing liquid structure, which can squeeze, arranges part
Liquid；When No.1 moving tooth and No. two moving teeth, which stir washing basket, makees circle rotation, full wafer blotting membrane can constantly pass through down with washing basket
Portion's solution；Liquid sufficiently can all be incubated for washing ProBloot membrane；In the position for having washing trough lower frame moving tooth, blotting membrane can be by moving tooth
It jacks up, the patch washing basket face of blotting membrane also can further obtain liquid and sufficiently be incubated for washing.
Further, it includes antibody collecting tank and waste collection slot that the liquid, which collects accumulator tank,.
Further, a variety of different arrangement modes, multiple device combination fortune can also be used in the single independent operating of the device
Row.
The device of the incubation carrying out washing treatment of the biological sample film or glue carries out biological sample and carries out being incubated for washing and recycling
The method of antibody, it is characterised in that: firstly, the various required liquid for being incubated for carrying out washing treatment are carried out to biological sample, prepackage
Add with user oneself, is placed in storing among the chamber of liquid for rotary liquid container combination in advance；Outside
Under the driving of portion's gear, the chamber that can store liquid is made certain angle deflection liquid and is overflowed, and partly or entirely enters swing type
The liquid collection trough of drum wash combination, circulation road under the V-type liquid through liquid collection trough side wall one end, the lower end of lower circulation road
Equipped with the liquid flowing outlet communicated with drum apparatus, liquid flows out into drum apparatus from liquid flowing outlet；Drum apparatus
Washing trough lower frame swings, and liquid comes into full contact with completion with biological sample in washing trough lower frame and is incubated for washing process；
It is incubated for after the completion of washing process, drum apparatus, rotation to all liq in drum apparatus is controlled by control device
In the antibody collecting tank for the liquid collection trough that can be disposed through the No.1 flow-guiding mouth injection supporting frame lower part below drum apparatus, this
Afterwards, washing incubation slot returns to horizontal position, and another solution in receptive liquid storage container washs week into next incubation
Phase.After the completion of washing cycle, washing incubation slot can go to No. two to all liq through the other side of other direction counterclockwise and lead
Head piece injects in the waste collection slot of the liquid collection trough of supporting frame lower part placement, in this way, in cycles, until washing is incubated for
It completes；
Finally, biological sample keeps wet in dynamic washing trough, until user takes out biological sample.
Beneficial effects of the present invention and feature are: 1, abandoning the complicated pipeline liquid pump system for being difficult cleaning；In view of using liquid
Diversity, and devise multiple solution tanks, by the multiple gears of multiple motor drivens, container individually controlled to each slot and is inclined
Rake angle.The preset various liquid in container, including the closing that user oneself filling or producer encapsulate in advance, antibody is washed
Wash solution；The evolution of container is driven to pour into various liquid as needed as manual operations by outer gear
It washes in the container of film；2, antibody-solutions recoverable, save the cost；3, it is abundant to be incubated for washing；4, barrel mast formula, which squeezes, increases liquid knot
Structure can be further reduced antibody-solutions requirement.
Detailed description of the invention:
Fig. 1 is the overall structure diagram of present pre-ferred embodiments；
Fig. 2 is the structural schematic diagram of the liquid container monomer of present pre-ferred embodiments；
Fig. 3 is the structural schematic diagram that liquid container monomer is combined in Fig. 2；
Fig. 4 is that the swing type roller of present pre-ferred embodiments washes the structural schematic diagram of film combination；
Fig. 5 is the structural schematic diagram of the washing trough upper ledge of present pre-ferred embodiments；
Fig. 6 is the structural schematic diagram of the washing trough lower frame of present pre-ferred embodiments；
Fig. 7 is the structural schematic diagram of V-type strip protrusion (number 41) on the supporting frame of present pre-ferred embodiments；
Fig. 8 is the structural schematic diagram that the Clip Reed of present pre-ferred embodiments is matched with positioning jump ring slot；
Fig. 9 is the arragement construction schematic diagram of present pre-ferred embodiments moving tooth；
Figure 10 is the structural schematic diagram of present pre-ferred embodiments tubular washing basket (number 221)；
Figure 11 is the structure of washed ProBloot membrane merging tubular washing basket (number 221) of present pre-ferred embodiments cylinder
Schematic diagram；
Figure 12 is the schematic diagram of liquid outflow in the combination of present pre-ferred embodiments swing type drum wash；
Figure 13 is the schematic diagram that present pre-ferred embodiments liquid collects accumulator tank withdrawal liquid；
Figure 14 is the schematic diagram after the wherein one side of present pre-ferred embodiments removal supporting frame；
Figure 15 is the structural schematic diagram that the multiple devices of present pre-ferred embodiments are combined；
Figure 16 is the structural schematic diagram that another multiple devices of present pre-ferred embodiments are combined；
Specific embodiment
Invention is further explained with reference to the accompanying drawing:
Such as Fig. 1, a kind of device of the incubation carrying out washing treatment for biological sample, supporting frame 4 including groove profile and in slot
The liquid arranged from bottom to up in the supporting frame 4 of type collects accumulator tank 3, swing type drum wash combination 2, the storage of rotary liquid
Deposit container combination 1, motor and control device；The rotary liquid container combination 1 is open and can drive in outside for upper end
Dynamic device drive backspin turns the container of pouring liquid；The swing type drum wash combination 2 includes for being incubated for biological sample
The drum apparatus 22 of washing and the washing trough upper ledge 21 for combining rotary liquid container liquid importing drum apparatus 22 in 1；
The liquid collects the bottom that accumulator tank 3 is located at supporting frame 4, and it includes antibody collecting tank 31 and waste liquid that liquid, which collects accumulator tank 3,
Collecting tank 32, the liquid poured out for collection cylinder device 22.
Specifically, biological sample is membranaceous biological sample such as ProBloot membrane, polyacrylamide gel.
Such as Fig. 2, Fig. 3, the device of the incubation carrying out washing treatment according to claim 1 for biological sample, the rotation
Rotatable liquid container combination 1 is made of the superposition of multiple liquid container monomers；Liquid container monomer includes upper end
The chamber 11 for storing liquid of opening drives through the axis hole 12 at 11 middle part of chamber, by the chamber of external driver device driving
Moving gear 13 and liner sample container 14 that be preinstalled with solution and that middle part is slotted；11 lower part of chamber of liquid can be stored in toothed
Arc, chamber driving gear 13 are meshed with 11 lower part of chamber and chamber 11 can be driven to revolve under the driving of external driver device
Turn, there is the flow-guiding mouth 15 for facilitating liquid to flow out in the side of 11 upper opening of chamber；Liner sample container 14 and the chamber that liquid can be stored
The inner wall shape of room 11, which matches to merge, to be placed among liquid container
The side view of each liquid container monomer is identical, but can have different thickness；；Each external container
Driving gear, it is staggered；Corresponding liquid container is respectively driven, its opening can be made to liquid container monomer
Extreme lower position allows liquid to flow out.
Such as Fig. 5, Fig. 6, Fig. 7, the upper end of the washing trough upper ledge 21 is liquid collection trough 211, one end of liquid collection trough 211
Equipped with circulation road 213 under the V-type liquid communicated with liquid collection trough, the lower end of lower circulation road 213 is equipped with and 22 phase of drum apparatus
Logical liquid flowing outlet 214；The other end of liquid collection trough 211 is equipped with V-type fixture block 215, and there is washing basket lower frame plug-in type in its underpart
Fixed short axle 212；A V-type strip protrusion 41, liquid collection trough 211 are respectively provided on two opposite medial surfaces of supporting frame 4
The V-type strip protrusion that can be caught in fixture block 215 on respective side,
Such as Fig. 4, Fig. 6, Fig. 7, the drum apparatus 22 includes tubular washing basket 221, washing basket external drive gear 222 and washing trough
Lower frame 223；Washing trough lower frame 223 includes the cambered surface 2232 positioned at the fluted disc of 223 two sides of washing trough lower frame and for connecting chainring, fluted disc
Medial surface be smooth plane；There are the gear teeth 2231 at the lateral surface edge of fluted disc, and the gear teeth 2231 can be with washing basket external drive gear
222 engagements；The center of two sides fluted disc is equipped with aperture 2234, is inserted into for fixed short axle 212 and liquid flowing outlet 214；Under washing trough
The lower wall of frame is the cambered surface 2232 for placement, and tubular washing basket 221 is installed in cambered surface 2232, cambered surface 2232 and left and right fluted disc phase
Even, it forms washing incubation slot and tubular washing basket 221 is driven to rotate；The both side edges of cambered surface 2232 have liquid guide flow mouth 2233；
Such as Fig. 8,215 lateral wall of fixture block is equipped with positioning jump ring slot 2151, is equipped with circlip in positioning jump ring slot 2151
Piece 2152；The lower part of Clip Reed 2152 is in for shape protrusion, and the top of Clip Reed 2152 is in inverted L shape, Clip Reed 2152 it is upper
In bayonet 2235 where end can be caught in V-type fixture block on the fluted disc of side；When Clip Reed is located in jump-ring slot, Clip Reed 2152 it is upper
End insertion bayonet 2235, locks the position of washing trough lower frame；When swing type roller washes 2 insertion Film washer supporting frame of film combination,
When 2152 lower part of Clip Reed is pressurized for shape protrusion, bayonet 2235 is deviate from the upper end of Clip Reed 2152, and at this moment washing trough lower frame 223 can not
Limited swing.
Such as Fig. 9, Figure 10, the tubular washing basket 221 is the tubular structure that diaphragm is rolled into, and is equipped with cylinder in tubular washing basket 221
Pillar, which squeezes, increases liquid structure 6, has a shaped opening on diaphragm, can be dialled by the angularly disposed No.1 in 211 lower end surface of liquid collection trough
Angularly disposed No. two moving teeth 2236 insertion on 2232 medial surface of tooth 216 and cambered surface, No.1 moving tooth 216 and No. two moving teeth 2236
Direction is all clockwise, or is all counterclockwise.
Such as Figure 11, when washing trough upper ledge is fixed, and washing trough lower frame 223 is driven by washing basket external drive gear 222, with center
Aperture 2234 is axle center, the limited rotary oscillation of both forward and reverse directions, so that tubular washing basket 221 is by No.1 moving tooth 216 and No. two moving teeth
2236 stir rotation in succession；It washs as follows with biological sample details in incubation slot: being incubated for washing ProBloot membrane 5, be placed patch
It is attached to the medial surface of washing basket, barrel mast formula squeezes increasing liquid structure 6 and is placed in washing basket；When having liquid in washing trough, liquid can converge
Collection is in the bottom of washing trough.ProBloot membrane can be attached to washing basket inner wall, and its underpart can be immersed among liquid；Barrel mast formula, which squeezes, to be increased
Liquid structure 6 can squeeze and arrange partially liq；When No.1 moving tooth 216 and No. two moving teeth 2236, which stir washing basket, makees circle rotation,
Full wafer blotting membrane can constantly pass through lower part solution with washing basket；Liquid sufficiently can all be incubated for washing ProBloot membrane；It is washing
The position of slot lower frame moving tooth, blotting membrane can be jacked up by moving tooth, and it is abundant that the patch washing basket face of blotting membrane also can further obtain liquid
It is incubated for washing.
Such as Figure 12~Figure 14, the device of the incubation carrying out washing treatment of the biological sample film or glue carries out biological sample and is incubated
The method educated washing and recycle antibody, it is characterised in that: firstly, carrying out being incubated for the various required of carrying out washing treatment to biological sample
Liquid, prepackage are added with user oneself, are placed in the chamber for storing liquid of rotary liquid container combination 1 in advance
Among room 11；Under the driving of outer gear, the chamber 11 that can store liquid is made certain angle deflection liquid and is overflowed, part or complete
Portion enters the liquid collection trough 211 of swing type drum wash combination 2, under the V-type liquid through 211 side wall one end of liquid collection trough
Circulation road 213, the lower end of lower circulation road 213 are equipped with the liquid flowing outlet 214 communicated with drum apparatus 22, and liquid is flowed out from liquid
Mouth 214 flows out into drum apparatus 22；The washing trough lower frame 223 of drum apparatus 22 swings, and liquid is in washing trough lower frame 223
Completion, which is come into full contact with, with biological sample is incubated for washing process；
It is incubated for after the completion of washing process, drum apparatus 22 is controlled by control device and is rotated clockwise according to Figure 13
All liq can inject the placement of supporting frame lower part through the No.1 flow-guiding mouth 2233 of 22 lower section of drum apparatus in drum apparatus 22
Liquid collection trough 3 antibody collecting tank 31 in (generally needed when using first antibody liquid using antibody collecting tank 31 carry out
Recycling), hereafter, washing incubation slot returns to horizontal position, another solution in receptive liquid storage container, into next incubation
Washing cycle.After the completion of washing cycle, when washing incubation slot can go to certain angle counterclockwise, all liq can be through the other side
No. two flow-guiding mouths 2237 injection supporting frame lower part placement liquid collection trough 3 waste collection slot 32 in, in this way, Zhou Erfu
Begin, is completed until washing is incubated for；
Finally, biological sample keeps moisture state in dynamic washing trough, until user takes out biological sample.
Such as Figure 15, Figure 16, the device of the incubation carrying out washing treatment for biological sample can single independent operating, can also
Using a variety of different arrangement modes, multiple device combined runnings.
Above-mentioned design just can reach following purpose 1, and liquid accumulation can be allowed in the bottom of washing incubation slot, it is anti-to reach reduction
Body usage amount.Antibody-solutions requirement can be further reduced by squeezing increasing liquid structure using barrel mast formula.2, by fixed washing trough
Frame and washing trough lower frame can be rolled, be driven with external drive gear, both allow to interact repeatedly in limited range.Make it by moving tooth
Interior tubular washing basket rotation then drives biological sample above to achieve the effect that dynamic is incubated for washing.3, in washing incubation slot
Interior moving tooth will push up on the biological sample in washing basket, and helped through biological sample attaches the incubation washing in face in tubular washing basket.
4, wash second feature of incubation slot: in small angle rotation, the both side edges for washing incubation slot have liquid guide flow mouth to be higher than it
Interior liquid level, liquid can be maintained in slot.And in big angle rotary, washing incubation slot can come down in torrents liquid respectively to its two sides
Body, for example the antibody liquid that comes down in torrents to 31 side of antibody collecting tank is recyclable reuses.
Basic principles and main features and advantages of the present invention of the invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The structural relation and principle of invention, without departing from the spirit and scope of the present invention, the present invention also have various change and
It improves, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended power
Sharp claim and its equivalent thereof.
Claims (10)
1. a kind of device of the incubation carrying out washing treatment for biological sample, including motor and control device, it is characterised in that: also wrap
The liquid for including the supporting frame (4) of groove profile and arranging from bottom to up in the supporting frame (4) of groove profile collects accumulator tank (3), pendulum
Dynamic formula drum wash combines (2), rotary liquid container combination (1)；The rotary liquid container combines (1)
Upper end is open and the container of pouring liquid can be rotated under external driver device drive；The swing type drum wash combines (2)
It is led including the drum apparatus (22) for being incubated for washing to biological sample and by liquid in rotary liquid container combination (1)
Enter the washing trough upper ledge (21) of drum apparatus (22)；The liquid collects the bottom that accumulator tank (3) are located at supporting frame (4), is used for
The liquid that collection cylinder device (22) is poured out.
2. the device of the incubation carrying out washing treatment according to claim 1 for biological sample, it is characterised in that: the biology
Sample is membranaceous biological sample.
3. the device of the incubation carrying out washing treatment according to claim 1 for biological sample, it is characterised in that: the rotation
Formula liquid container combines (1) and is made of the superposition of multiple liquid container monomers；Liquid container monomer includes upper end
Opening the chamber (11) for storing liquid, through in the middle part of chamber (11) axis hole (12), by external driver device driving
Chamber driving gear (13) and liner sample container (14) that be preinstalled with solution and that middle part is slotted；The chamber (11) of liquid can be stored
Lower part is in toothed arc, and chamber driving gear (13) is meshed with chamber (11) lower part and can be in the driving of external driver device
Lower drive chamber (11) rotation, there is the flow-guiding mouth (15) for facilitating liquid to flow out in the side of chamber (11) upper opening；Liner sample holds
Device (14) merges with the matching of the inner wall shape for the chamber (11) that can store liquid to be placed among liquid container.
4. the device of the incubation carrying out washing treatment according to claim 3 for biological sample, it is characterised in that: described each
The side view of liquid container monomer is identical, but can have different thickness；；The driving gear of each external container, is staggered
Arrangement；Corresponding liquid container is respectively driven, its opening can be made to arrive the extreme lower position of liquid container monomer, allow liquid
Outflow.
5. the device of the incubation carrying out washing treatment according to claim 1 for biological sample, it is characterised in that: the washing trough
The upper end of upper ledge (21) is liquid collection trough (211), and one end of liquid collection trough (211) is equipped with the V communicated with liquid collection trough
Circulation road (213) under type liquid, the lower end of lower circulation road (213) are equipped with the liquid flowing outlet communicated with drum apparatus (22)
(214)；The other end of liquid collection trough (211) is equipped with V-type fixture block (215), and there is washing basket lower frame plug-in mounting short axle in its underpart
(212)；A V-type strip protrusion (41), liquid collection trough (211) are respectively provided on two opposite medial surfaces of supporting frame (4)
The V-type strip protrusion on respective side can be caught in fixture block (215), the drum apparatus (22) include tubular washing basket (221),
Washing basket external drive gear (222) and washing trough lower frame (223)；Washing trough lower frame (223) includes being located at washing trough lower frame (223) two sides
Fluted disc and cambered surface (2232) for connecting chainring, the medial surface of fluted disc are smooth plane；There is wheel at the lateral surface edge of fluted disc
Tooth (2231), the gear teeth (2231) can be engaged with washing basket external drive gear (222)；The center of two sides fluted disc is equipped with aperture
(2234), for fixed short axle (212) and liquid flowing outlet (214) insertion；The lower wall of washing trough lower frame is the cambered surface for placement
(2232), tubular washing basket (221) is installed in cambered surface (2232), and cambered surface (2232) is connected with left and right fluted disc, is formed washing and is incubated for
Slot simultaneously drives tubular washing basket (221) to rotate；The both side edges of cambered surface (2232) have liquid guide flow mouth (2233)
6. the device of the incubation carrying out washing treatment according to claim 5 for biological sample, it is characterised in that: fixture block
(215) lateral wall is equipped with positioning jump ring slot (2151), is equipped with Clip Reed (2152) in positioning jump ring slot (2151)；Clip Reed
(2152) lower part is in for shape protrusion, and the top of Clip Reed (2152) is in inverted L shape, and the upper end of Clip Reed (2152) can be caught in
In bayonet (2235) where V-type fixture block on the fluted disc of side；When Clip Reed is located in jump-ring slot, the upper end of Clip Reed (2152) is inserted
Card inlet (2235) locks the position of washing trough lower frame；When swing type roller washes film combination (2) insertion Film washer supporting frame,
When Clip Reed (2152) lower part is pressurized for shape protrusion, bayonet (2235) are deviate from the upper end of Clip Reed (2152), at this moment washing trough lower frame
(223) swing that can be unrestricted.
7. the device of the incubation carrying out washing treatment according to claim 4 for biological sample, it is characterised in that: the tubular
Washing basket (221) is the tubular structure that diaphragm is rolled into, and is equipped with barrel mast formula in tubular washing basket (221) and squeezes increasing liquid structure (6), film
On piece has a shaped opening, can be by the angularly disposed No.1 moving tooth (216) in liquid collection trough (211) lower end surface and cambered surface (2232)
No. two angularly disposed moving teeth (2236) insertion on side, No.1 moving tooth (216) and the direction of No. two moving teeth (2236) are all suitable
Hour hands, or be all counterclockwise.
When washing trough upper ledge is fixed, and washing trough lower frame (223) is driven by washing basket external drive gear (222), with central openings
It (2234) is axle center, the limited rotary oscillation of both forward and reverse directions, so that tubular washing basket (221) is dialled by No.1 moving tooth (216) and No. two
Tooth (2236) stirs rotation in succession；It washs as follows with biological sample details in incubation slot: being incubated for washing ProBloot membrane (5), quilt
Placement is attached to the medial surface of washing basket, and barrel mast formula squeezes increasing liquid structure (6) and is placed in washing basket；When having liquid in washing trough,
Liquid can collect in the bottom of washing trough.ProBloot membrane can be attached to washing basket inner wall, and its underpart can be immersed among liquid；Barrel mast
Formula extruding increasing liquid structure (6), which can squeeze, arranges partially liq；When No.1 moving tooth (216) and No. two moving teeth (2236) stir washing basket
When making circle rotation, full wafer blotting membrane can constantly pass through lower part solution with washing basket；Liquid sufficiently can all be incubated for washing albumen
Blotting membrane；In the position for having washing trough lower frame moving tooth, blotting membrane can be jacked up by moving tooth, and the patch washing basket face of blotting membrane also can be further
It obtains liquid and is sufficiently incubated for washing.
8. the device of the incubation carrying out washing treatment according to claim 6 for biological sample, it is characterised in that: the liquid
Collecting accumulator tank (3) includes antibody collecting tank (31) and waste collection slot (32).
9. the device with the incubation carrying out washing treatment for biological sample of claim 1~8 any claim, special
Sign is: the single independent operating of the device, and a variety of different arrangement modes, multiple device combined runnings can also be used.
10. being carried out using the device of the incubation carrying out washing treatment of biological sample film or glue described in claim 1~8 any claim
Biological sample carries out the method for being incubated for washing and recycling antibody, it is characterised in that: firstly, carrying out incubation washup to biological sample
The various required liquid of reason, prepackage are added with user oneself, are placed in rotary liquid container combination (1) in advance
The chamber (11) for storing liquid among；Under the driving of outer gear, the chamber (11) that can store liquid makees certain angle
It deflects liquid to overflow, partly or entirely enters the liquid collection trough (211) of swing type drum wash combination (2), received through liquid
Collect circulation road (213) under the V-type liquid of slot (211) side wall one end, the lower end of lower circulation road (213) is equipped with and drum apparatus (22)
The liquid flowing outlet (214) communicated, liquid flow out into drum apparatus (22) from liquid flowing outlet (214)；Drum apparatus
(22) washing trough lower frame (223) swings, and liquid comes into full contact with completion incubation with biological sample in washing trough lower frame (223) and washes
Wash process；
It is incubated for after the completion of washing process, drum apparatus (22) is controlled by control device, are owned in rotation to drum apparatus (22)
Liquid can inject the liquid collection trough (3) of supporting frame lower part placement through the No.1 flow-guiding mouth (2233) below drum apparatus (22)
Antibody collecting tank (31) in, hereafter, washing incubation slot returns to horizontal position, another solution in receptive liquid storage container,
Into next incubation washing cycle.After the completion of washing cycle, washing incubation slot can go to other direction to all counterclockwise
The waste collection slot of the liquid collection trough (3) of No. two flow-guiding mouths (2237) injection supporting frame lower part placement of the liquid through the other side
(32) in, in this way, in cycles, being completed until washing is incubated for；
Finally, biological sample keeps wet in dynamic washing trough, until user takes out biological sample.
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| CN201910029780.5A CN109719070B (en) | 2019-01-14 | 2019-01-14 | Device for incubation washing treatment of biological sample and incubation and antibody recovery method thereof |
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| CN109719070B CN109719070B (en) | 2023-04-07 |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005000866A (en) * | 2003-06-13 | 2005-01-06 | Sankyu Inc | Cleaning device for packed material for column and its cleaning method |
| CN201195732Y (en) * | 2008-05-23 | 2009-02-18 | 江苏省冶金设计院有限公司 | Pin-tooth transmission type blast furnace mixer |
| US20130263898A1 (en) * | 2010-11-05 | 2013-10-10 | Hideo Takei | Rotating drum type work washing apparatus |
| CN103658095A (en) * | 2013-12-09 | 2014-03-26 | 哈尔滨安赛福经贸有限公司 | Washing device of labor protection safety net |
| CN103913365A (en) * | 2014-04-04 | 2014-07-09 | 田丰 | Automatic processor of biological sample film |
| CN108570711A (en) * | 2018-06-05 | 2018-09-25 | 浙江羿阳太阳能科技有限公司 | A kind of polycrystalline silicon material acid dip pickle |
| CN108906756A (en) * | 2018-08-03 | 2018-11-30 | 芜湖英特杰智能科技有限公司 | A kind of computer host box high-effective disinfecting equipment |
| CN208327382U (en) * | 2018-05-24 | 2019-01-04 | 中国石油天然气股份有限公司 | Dumping device for liquid |
-
2019
- 2019-01-14 CN CN201910029780.5A patent/CN109719070B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005000866A (en) * | 2003-06-13 | 2005-01-06 | Sankyu Inc | Cleaning device for packed material for column and its cleaning method |
| CN201195732Y (en) * | 2008-05-23 | 2009-02-18 | 江苏省冶金设计院有限公司 | Pin-tooth transmission type blast furnace mixer |
| US20130263898A1 (en) * | 2010-11-05 | 2013-10-10 | Hideo Takei | Rotating drum type work washing apparatus |
| CN103658095A (en) * | 2013-12-09 | 2014-03-26 | 哈尔滨安赛福经贸有限公司 | Washing device of labor protection safety net |
| CN103913365A (en) * | 2014-04-04 | 2014-07-09 | 田丰 | Automatic processor of biological sample film |
| CN208327382U (en) * | 2018-05-24 | 2019-01-04 | 中国石油天然气股份有限公司 | Dumping device for liquid |
| CN108570711A (en) * | 2018-06-05 | 2018-09-25 | 浙江羿阳太阳能科技有限公司 | A kind of polycrystalline silicon material acid dip pickle |
| CN108906756A (en) * | 2018-08-03 | 2018-11-30 | 芜湖英特杰智能科技有限公司 | A kind of computer host box high-effective disinfecting equipment |
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| CN109719070B (en) | 2023-04-07 |
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Effective date of registration: 20211214 Address after: 430073 Room 518, building B1, R & D building, zone B, C and D, Wuhan National biological industry base project, 666 Gaoxin Avenue, Donghu New Technology Development Zone, Wuhan City, Hubei Province Applicant after: Wuhan Taiwo Technology Co.,Ltd. Address before: No.12, no.395-043, Luoshi Road, Hongshan District, Wuhan City, Hubei Province, 430000 Applicant before: Wang Guofu Applicant before: Liu suheng |
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