CN109718301A - Compound Chinese wampee leaf oral solution and preparation method - Google Patents
Compound Chinese wampee leaf oral solution and preparation method Download PDFInfo
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Abstract
The invention discloses a kind of compound Chinese wampee leaf oral solution and preparation methods, comprising: 700-900 parts of Chinese wampee leaf, 40-60 parts of wrinkled giant hyssop, 18-22 parts of peppermint, 48-52 parts of Radix Isatidis, 48-52 parts of glutinous rehmannia；2-2.5 parts of simple syrups, 1-1.5 parts of potassium sorbates, 0.5-1 parts of polyoxyethylene sorbitan monoleates；Production method includes: that distillation is extracted, repeatedly decocted, concentration, refrigeration addition filler, stirring evenly, encapsulating, sterilizing, to obtain the final product.The present invention has nontoxic, and safely and effectively, antibiotic-free, natural environmental-protective, active constituent content is high, quick feature, and it saves convenient, does not need deepfreeze, preparation method is simple, effectively, is suitable for industrialized production.
Description
Technical field
The present invention relates to Chinese wampee leaf oral solution preparation fields.It is more particularly related to a kind of anti-treatment influenza, stream
Brain compound Chinese wampee leaf oral solution and preparation method.
Background technique
Chinese wampee leaf, Chinese medicine name；This product is the blade of rutaceae Calusena lansium, and whole year can adopt；Chinese wampee leaf is dissipated with inducing diaphoresis
The effect of heat, qi-regulating and phlegm-dissipating.The herbal mixture of flu-prevention, traditional Chinese medicinal decoction are made using Chinese wampee leaf as medicinal material by few people
Agent is all that decoction of medicinal ingredients effective component be brewed into and that it is boiled into is low, and the effective component in Chinese medicine is easy to volatilize during heating
It is lost, the frequency for eating decoction of medicinal ingredients is high, slow effect；Secondly, the volatile oil in the existing oral solution containing Volatile Oil of Medicinal Herbs is effective
Ingredient is easy to oxidize rotten or volatilizees, therefore needs cryo-conservation, very inconvenient.
Summary of the invention
The present invention, which a further object is, provides a kind of compound Chinese wampee leaf oral solution and preparation method, nontoxic, safely and effectively,
Antibiotic-free, natural environmental-protective, active constituent content is high, quick, and it is saved conveniently, does not need deepfreeze.
In order to realize these purposes and other advantages according to the present invention, a kind of compound Chinese wampee leaf oral solution is provided, is wrapped
Include following raw material: 700-900 parts of Chinese wampee leaf, 40-60 parts of wrinkled giant hyssop, 18-22 parts of peppermint, 48-52 parts of Radix Isatidis, glutinous rehmannia
48-52 parts.
It preferably, further include parts by weight meter: 2-2.5 parts of simple syrups, 1-1.5 parts of potassium sorbates, 0.5-1 portions of poly- sorbs
Ester 80.
The present invention also provides the preparation methods of above-mentioned compound Chinese wampee leaf oral solution, the specific steps are as follows:
Step 1: the Chinese wampee leaf of above-mentioned parts by weight, wrinkled giant hyssop, peppermint distillation are extracted, the dregs of a decoction, aqueous solution and volatile oil are obtained；
Step 2: the dregs of a decoction and the Radix Isatidis of above-mentioned parts by weight, glutinous rehmannia are mixed evenly, mixture is obtained, to mixture
The middle water that 2 times of its total weight is added, 80-120 DEG C of decoction 5h are obtained by filtration the first decocting liquid and the first residue, then to the first residue
The second decocting liquid is obtained by filtration in the middle water that 2 times of its total weight is added, 150-220 DEG C of decoction 30min, merges the first decocting liquid and second
Decocting liquid obtains total filtrate, and total filtrate merges to obtain mixed liquor with aqueous solution obtained in step 1, and mixed liquor is concentrated into original volume
20%, add ethyl alcohol to make the 62-68% of wt containing amount of alcohol, refrigerate 40-56 hour, filtering obtains refrigerating liquid, dense by liquid is refrigerated
It is reduced to the 35% of original volume, obtains concentrate；
Step 3: the simple syrup of above-mentioned parts by weight, potassium sorbate are added into concentrate, 40 DEG C of heating water baths, stirring is
Even 3 DEG C of refrigeration 2h, filters to get filtrate, then volatile oil, the polyoxyethylene sorbitan monoleate of above-mentioned parts by weight and water is added to 1000 into filtrate
Parts by weight stir evenly, encapsulating, sterilizing to get.
Preferably, Chinese wampee leaf, wrinkled giant hyssop, peppermint will be pre-processed before step 1, by Chinese wampee leaf, wrinkled giant hyssop, Herba Menthae powder
It is broken into 20-30 mesh powder, the powder of uniformly mixed Chinese wampee leaf, wrinkled giant hyssop, peppermint is clipped between two layers of gauze, two layers of gauze
It is clipped between two panels metal mesh.
Preferably, the steam used in distillating extracting oil in step 1 for volume fraction be 98% water vapour and
The mixed vapour for the alcohol vapour that volume fraction is 2%.
Preferably, the volatile oil in step 3 needs before adding by stabilizer treatment, using centrifugal spray dryer
Drying prescription is dry, obtains microencapsulation volatile oil, the stabilizer includes the raw material of following parts by weight: 2 parts of Divine Comedy, 2 parts of Radix Glycyrrhizae, Pu Gong
1 part of English, 35 parts of pueraria lobata.
Preferably, the stabilizer specific the preparation method comprises the following steps:
S1, the chopping of the Divine Comedy of above-mentioned parts by weight, Radix Glycyrrhizae and pueraria lobata is uniformly mixed to obtain object to be extracted；
1 part of potassium sorbate of parts by weight meter, 1 part of acetic acid and 98 parts of deionized waters are uniformly mixed and are dissolved to clarification by S2, are obtained
Extracting solution；
S3, the extracting solution that 1.2 times of its total weight parts are then added into object to be extracted, are heated to 80 DEG C, extract 3h, constant temperature
It filters to get filtrate, the dandelion of above-mentioned parts by weight, constant temperature insulated and stirred 1h, constant temperature mistake is added when filtrate is cooled to 60 DEG C
Filter, filtrate is stabilizer.
The present invention is include at least the following beneficial effects:
The first, using Chinese medicines such as Chinese wampee leaf, wrinkled giant hyssop, peppermint, Radix Isatidis, glutinous rehmannia, the oral solution being prepared has anti-
The effect of influenza, and significant effect；
The second, the more uniform distillation efficiency for fully ensuring that medicinal material that vapor can be made to heat by metal mesh heating,
The effective component and quality for guaranteeing volatile oil, improve the extraction efficiency of volatile oil；
Third passes through addition Divine Comedy, Radix Glycyrrhizae, dandelion and pueraria lobata, will volatilize oil and is stabilized, waving for microencapsulation is made
Hair oil inclusion compound, improves the stability of volatile oil, while the curative effect of the oral solution of guarantee, reduces the storage item of oral solution
Part avoids the harsh conditions of the stored under refrigeration of oral solution needs.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
<embodiment 1>
A kind of compound Chinese wampee leaf oral solution, including following raw material: 700 parts of Chinese wampee leaf, 40 parts of wrinkled giant hyssop, peppermint 18
Part, 48 parts of Radix Isatidis, 48 parts of glutinous rehmannia；
Specific preparation step is as follows:
Step 1: the Chinese wampee leaf of above-mentioned parts by weight, wrinkled giant hyssop, peppermint distillation are extracted, the dregs of a decoction, aqueous solution and volatile oil are obtained；
Step 2: the dregs of a decoction and the Radix Isatidis of above-mentioned parts by weight, glutinous rehmannia are mixed evenly, mixture is obtained, to mixture
The middle water that 2 times of its total weight is added, 80-120 DEG C of decoction 5h are obtained by filtration the first decocting liquid and the first residue, then to the first residue
The second decocting liquid is obtained by filtration in the middle water that 2 times of its total weight is added, 150-220 DEG C of decoction 30min, merges the first decocting liquid and second
Decocting liquid obtains total filtrate, and total filtrate merges to obtain mixed liquor with aqueous solution obtained in step 1, and mixed liquor is concentrated into original volume
20%, add ethyl alcohol to make the 62-68% of wt containing amount of alcohol, refrigerated 48 hours under conditions of -3-3 DEG C, filter, refrigerated
Refrigeration liquid is concentrated into the 35% of original volume, obtains concentrate by liquid；
Step 3: the simple syrup of above-mentioned parts by weight, potassium sorbate are added into concentrate, 40 DEG C of heating water baths, stirring is
Even, 3 DEG C of refrigeration 2h filter to get filtrate, then volatile oil, the polyoxyethylene sorbitan monoleate of above-mentioned parts by weight and water is added to 1000 into filtrate
Parts by weight stir evenly, encapsulating, sterilizing to get.
<embodiment 2>
A kind of compound Chinese wampee leaf oral solution, including following raw material: 900 parts of Chinese wampee leaf, 60 parts of wrinkled giant hyssop, peppermint 22
Part, 52 parts of Radix Isatidis, 52 parts of glutinous rehmannia；
Specific preparation step is as follows:
Step 1: the Chinese wampee leaf of above-mentioned parts by weight, wrinkled giant hyssop, peppermint distillation are extracted, the dregs of a decoction, aqueous solution and volatile oil are obtained；
Step 2: the dregs of a decoction and the Radix Isatidis of above-mentioned parts by weight, glutinous rehmannia are mixed evenly, mixture is obtained, to mixture
The middle water that 2 times of its total weight is added, 80-120 DEG C of decoction 5h are obtained by filtration the first decocting liquid and the first residue, then to the first residue
The second decocting liquid is obtained by filtration in the middle water that 2 times of its total weight is added, 150-220 DEG C of decoction 30min, merges the first decocting liquid and second
Decocting liquid obtains total filtrate, and total filtrate merges to obtain mixed liquor with aqueous solution obtained in step 1, and mixed liquor is concentrated into original volume
20%, add ethyl alcohol to make the 62-68% of wt containing amount of alcohol, refrigerated 40 hours under conditions of -3-3 DEG C, filter, refrigerated
Refrigeration liquid is concentrated into the 35% of original volume, obtains concentrate by liquid；
Step 3: the simple syrup of above-mentioned parts by weight, potassium sorbate are added into concentrate, 40 DEG C of heating water baths, stirring is
Even, 3 DEG C of refrigeration 2h filter to get filtrate, then volatile oil, the polyoxyethylene sorbitan monoleate of above-mentioned parts by weight and water is added to 1000 into filtrate
Parts by weight stir evenly, encapsulating, sterilizing to get.
<embodiment 3>
A kind of compound Chinese wampee leaf oral solution, including following raw material: 800 parts of Chinese wampee leaf, 50 parts of wrinkled giant hyssop, peppermint 20
Part, 50 parts of Radix Isatidis, 50 parts of glutinous rehmannia；
Specific preparation step is as follows:
Step 1: the Chinese wampee leaf of above-mentioned parts by weight, wrinkled giant hyssop, peppermint distillation are extracted, the dregs of a decoction, aqueous solution and volatile oil are obtained；
Step 2: the dregs of a decoction and the Radix Isatidis of above-mentioned parts by weight, glutinous rehmannia are mixed evenly, mixture is obtained, to mixture
The middle water that 2 times of its total weight is added, 80-120 DEG C of decoction 5h are obtained by filtration the first decocting liquid and the first residue, then to the first residue
The second decocting liquid is obtained by filtration in the middle water that 2 times of its total weight is added, 150-220 DEG C of decoction 30min, merges the first decocting liquid and second
Decocting liquid obtains total filtrate, and total filtrate merges to obtain mixed liquor with aqueous solution obtained in step 1, and mixed liquor is concentrated into original volume
20%, add ethyl alcohol to make the 62-68% of wt containing amount of alcohol, refrigerated 56 hours under conditions of -3-3 DEG C, filter, refrigerated
Refrigeration liquid is concentrated into the 35% of original volume, obtains concentrate by liquid；
Step 3: the simple syrup of above-mentioned parts by weight, potassium sorbate are added into concentrate, 40 DEG C of heating water baths, stirring is
Even, 3 DEG C of refrigeration 2h filter to get filtrate, then volatile oil, the polyoxyethylene sorbitan monoleate of above-mentioned parts by weight and water is added to 1000 into filtrate
Parts by weight stir evenly, encapsulating, sterilizing to get.
<embodiment 4>
A kind of compound Chinese wampee leaf oral solution, including following raw material: 800 parts of Chinese wampee leaf, 50 parts of wrinkled giant hyssop, peppermint 20
Part, 50 parts of Radix Isatidis, 50 parts of glutinous rehmannia；
Specific preparation step is as follows:
Chinese wampee leaf, wrinkled giant hyssop, peppermint will be pre-processed, and Chinese wampee leaf, wrinkled giant hyssop, peppermint are ground into 20-30 mesh powder, will
Uniformly mixed Chinese wampee leaf, wrinkled giant hyssop, peppermint powder be clipped between two layers of gauze, two layers of gauze clip is between two panels metal mesh；
Step 1: obtaining the dregs of a decoction, aqueous solution and waving the Chinese wampee leaf of above-mentioned parts by weight, wrinkled giant hyssop, peppermint distillating extracting oil
Hair oil, the steam wherein used in distillating extracting oil for volume fraction be 98% water vapour and volume fraction be 2%
The mixed vapour of alcohol vapour；
Step 2: the dregs of a decoction and the Radix Isatidis of above-mentioned parts by weight, glutinous rehmannia are mixed evenly, mixture is obtained, to mixture
The middle water that 2 times of its total weight is added, 80-120 DEG C of decoction 5h are obtained by filtration the first decocting liquid and the first residue, then to the first residue
The second decocting liquid is obtained by filtration in the middle water that 2 times of its total weight is added, 150-220 DEG C of decoction 30min, merges the first decocting liquid and second
Decocting liquid obtains total filtrate, and total filtrate merges to obtain mixed liquor with aqueous solution obtained in step 1, and mixed liquor is concentrated into original volume
20%, add ethyl alcohol to make the 62-68% of wt containing amount of alcohol, refrigerated 48 hours under conditions of -3-3 DEG C, filter, refrigerated
Refrigeration liquid is concentrated into the 35% of original volume, obtains concentrate by liquid；
Step 3: the simple syrup of above-mentioned parts by weight, potassium sorbate are added into concentrate, 40 DEG C of heating water baths, stirring is
Even 3 DEG C of refrigeration 2h, filters to get filtrate, then volatile oil, the polyoxyethylene sorbitan monoleate of above-mentioned parts by weight and water is added to 1000 into filtrate
Parts by weight stir evenly, encapsulating, sterilizing to get.
<embodiment 5>
A kind of compound Chinese wampee leaf oral solution, including following raw material: 800 parts of Chinese wampee leaf, 50 parts of wrinkled giant hyssop, peppermint 20
Part, 50 parts of Radix Isatidis, 50 parts of glutinous rehmannia；
Specific preparation step is as follows:
Chinese wampee leaf, wrinkled giant hyssop, peppermint will be pre-processed, and Chinese wampee leaf, wrinkled giant hyssop, peppermint are ground into 20-30 mesh powder, will
Uniformly mixed Chinese wampee leaf, wrinkled giant hyssop, peppermint powder be clipped between two layers of gauze, two layers of gauze clip is between two panels metal mesh；
Step 1: obtaining the dregs of a decoction, aqueous solution and waving the Chinese wampee leaf of above-mentioned parts by weight, wrinkled giant hyssop, peppermint distillating extracting oil
Hair oil, the steam wherein used in distillating extracting oil for volume fraction be 98% water vapour and volume fraction be 2%
The mixed vapour of alcohol vapour；
Step 2: the dregs of a decoction and the Radix Isatidis of above-mentioned parts by weight, glutinous rehmannia are mixed evenly, mixture is obtained, to mixture
The middle water that 2 times of its total weight is added, 80-120 DEG C of decoction 5h are obtained by filtration the first decocting liquid and the first residue, then to the first residue
The second decocting liquid is obtained by filtration in the middle water that 2 times of its total weight is added, 150-220 DEG C of decoction 30min, merges the first decocting liquid and second
Decocting liquid obtains total filtrate, and total filtrate merges to obtain mixed liquor with aqueous solution obtained in step 1, and mixed liquor is concentrated into original volume
20%, add ethyl alcohol to make the 62-68% of wt containing amount of alcohol, refrigerated 48 hours under conditions of -3-3 DEG C, filter, refrigerated
Refrigeration liquid is concentrated into the 35% of original volume, obtains concentrate, wherein mixed liquor is concentrated after obtaining mixed liquor by liquid
Addition ethyl alcohol makes the ethanol content wt 62-68% in solution, can be effectively reduced the solidification of mixed liquor by adding ethyl alcohol
Point prevents mixed liquor from causing freezing solution after lower than 0 DEG C, and volume expansion causes the effective component of drug to change, works as medicine
Object needs to carry out fast cooling after being heated or being heated concentration, prevents drug pharmacological property during slow cooling from changing
Or by the germ contamination in environment, drug occurs rotten；
Step 3: the simple syrup of above-mentioned parts by weight, potassium sorbate are added into concentrate, 40 DEG C of heating water baths, stirring is
Even 3 DEG C of refrigeration 2h, filters to get filtrate, then volatile oil, the polyoxyethylene sorbitan monoleate of above-mentioned parts by weight and water is added to 1000 into filtrate
Parts by weight stir evenly, encapsulating, sterilizing to get.
Wherein, the volatile oil in step 3 is needed before addition by stabilizer treatment, i.e., by stabilizer and volatile oil
It is mixed evenly, it is dry using centrifugal spray dryer drying prescription, microencapsulation volatile oil is obtained, the stabilizer includes following parts by weight
Raw material: 2 parts of Divine Comedy, 2 parts of Radix Glycyrrhizae, 1 part of dandelion, 35 parts of pueraria lobata.
The stabilizer specific the preparation method comprises the following steps:
S1, the chopping of the Divine Comedy of above-mentioned parts by weight, Radix Glycyrrhizae and pueraria lobata is uniformly mixed to obtain object to be extracted；
1 part of potassium sorbate of parts by weight meter, 1 part of acetic acid and 98 parts of deionized waters are uniformly mixed and are dissolved to clarification by S2, are obtained
Extracting solution；
S3, the extracting solution that 1.2 times of its total weight parts are then added into object to be extracted, are heated to 80 DEG C, extract 3h, constant temperature
It filters to get filtrate, the dandelion of above-mentioned parts by weight, constant temperature insulated and stirred 1h, constant temperature mistake is added when filtrate is cooled to 60 DEG C
Filter, filtrate is stabilizer.
<comparative example 1>
According to traditional Traditional medicine infusion method, by 50 parts of wrinkled giant hyssop, 20 parts of peppermint, 50 parts of Radix Isatidis, 50 parts of glutinous rehmannia, mixing is equal
It is even, it is put into silo, the water of whole 2 times of total weight of Chinese medicines, 80-120 DEG C of decoction 5h is added, filtering obtains the first decocting liquid and medicine
Slag, the dregs of a decoction add the water of 2 times of its total weight again, and 150-220 DEG C of decoction 30min obtains the second decocting liquid, merge the first decocting liquid and second
Decocting liquid, heating are concentrated into the 35% of original volume, obtain Chinese wampee leaf traditional Chinese herbal decoction.
<comparative example 2>
According to traditional Traditional medicine infusion method, by 800 parts of Chinese wampee leaf, 50 parts of wrinkled giant hyssop, 20 parts of peppermint, 50 parts of Radix Isatidis,
It is 50 parts yellow, it is uniformly mixed, is put into silo, the water of whole 2 times of total weight of Chinese medicines, 80-120 DEG C of decoction 5h is added, filtering obtains
First decocting liquid and the dregs of a decoction, the dregs of a decoction add the water of 2 times of its total weight again, 150-220 DEG C of decoction 30min, obtain the second decocting liquid, merge the
One decocting liquid and the second decocting liquid, heating are concentrated into the 35% of original volume, obtain Chinese wampee leaf traditional Chinese herbal decoction.
Experimental example:
The curative effect of the resisiting influenza virus hypotype of compound Chinese wampee leaf oral solution
Carry out drug toxicity measurement and virus EID in advance before experiment starts50Measurement, two experiment specific experiment steps
It omits, drug toxicity measurement uses the drug of embodiment 1 to be detected for sample, and drug makees chicken embryo without acute toxicity as the result is shown
With viral EID50Measurement result calculation method be by Reed-MuenchShi method calculate influenza virus H1N1 caused by
EID50, and positive lesion concentration is obtained 10-5~10-6Between, it calculates and looks into antilogarithm table and obtain chicken embryo 50 3nfective dose
(EID50) it is 10-5.84/ 0.1mL, i.e., 10-5.84Should be diluted by looking into antilogarithm table and obtaining virus liquid by 140000 times.
(1) experimental principle: being host with normal chicken embryo, and measurement traditional Chinese medicine sample inhibits chicken embryo caused by influenza virus H1N1
Lesion degree.
(2) test material and method: decoction made from oral solution made from Example 2~5 and comparative example 1；
Except normal chicken embryo control group with no treatment in addition to, remaining each group is handled as follows:
Medicine group: first through allantoic cavity virus infection 20ul (20EID50), set chicken embryo in 37 DEG C incubator 60 minutes, then take
Out, every embryo allantoic cavity injects drug 20ul again.
Saline control group: in allantoic cavity saline injection 20ul, chicken embryo is set in 37 DEG C of incubators.
Virus control group: prior to allantoic cavity virus infection liquid 20ul (20EID50), it sets embryo in 37 DEG C of incubators after sixty minutes, takes
For allantoic cavity saline injection 20ul.
After the above egg injection, with paraffin closed injection hole is dissolved, it is placed in 37 DEG C of incubator cultures.Picking up ovum daily
It is observed one by one under lamp 2 times, died discards in 24 hours, until 48 hours, the chicken embryo of survival is moved into 4 DEG C of refrigerator overnights, next day
Allantoic fluid is harvested respectively.Hemagglutination test (HA test) is carried out with 0.5% chicken red blood cell.
H1N1 plants of resisiting influenza virus of blood coagulation tests result in table 1, Chinese medicine extract chicken embryo
| Allantoic fluid dilution | Stoste | 1:2 | 1:2-2 | 1:2-3 | 1:2-4 | 1:2-5 | 1:2-6 | 1:2-7 |
| Normal group | - | - | - | - | - | - | - | - |
| Virus control group | + | + | + | + | + | + | + | + |
| Embodiment 2 | + | + | + | + | + | - | - | - |
| Embodiment 3 | + | + | + | + | + | - | - | - |
| Embodiment 4 | + | + | + | + | + | - | - | - |
| Embodiment 5 | + | + | + | + | - | - | - | - |
| Comparative example 1 | + | + | + | + | + | + | + | - |
HA on+expression influenza virus envelopes is in conjunction with the specific receptor on chicken red blood cell film, to red blood cell occur
Agglutination, i.e. hemagglutination test are positive.(positive illustrates that drug does not destroy or be adsorbed in influenza virus HA under the concentration, i.e. the medicine
Object infected by influenza is invalid.)
Indicate that not in conjunction with the specific receptor on chicken red blood cell, it is solidifying not occur red blood cell by the HA on influenza virus envelopes
Collection, i.e. hemagglutination test are negative.(feminine gender illustrates that drug destroys or be adsorbed in influenza virus HA under the concentration, i.e. drug has suppression
The effect of influenza virus processed.)
Influenza virus can rise in value into the cell in chick chorioallantoic membrane, and release into allantoic fluid, in allantoic fluid
Influenza virus can make human red blood cells or certain animal erythrocytes that macroscopic hemagglutination occur under certain condition.
After infusion of medicine allantoic cavity, the influenza virus in allantoic fluid cannot make red blood cell blood clotting phenomenon occur, then it represents that the drug
Infected by influenza is inhibited.
The instar chicken embryos on the 10th that the movement of this experimental selection is active, development produces pay attention to carrying out chicken embryo during the experiment
Warming, the collection of chick embryo allantoic liquid all carries out on ice, to guarantee the accuracy of experimental result.Experimental principle is influenza virus
It can be grown in chicken embryo and replicate and cause lesion or lead to chicken embryo death.
As shown in Table 1, the hemagglutinative titer position 2 of influenza virus allantoic fluid-7, embodiment 2~4 make hirst's hemagglutination potency from
2-7It is reduced to 2-4；Embodiment 5 makes hirst's hemagglutination potency from 2-7It is reduced to 2-3；Comparative example 1 makes hirst's hemagglutination potency from 2-7
It is reduced to 2-6,, due to having lacked this medicinal material simply of Chinese wampee leaf in comparative example, comparative example 1 is caused to only have the effect of micro anti influenza；
These drugs remain to the hemagglutinin structure function with influenza virus in chicken embryo, destroy or be adsorbed in influenza virus hemagglutinin knot
Structure inhibits it to hinder the duplication of influenza virus in conjunction with erythrocyte surface sialic acid receptor, achievees the purpose that control influenza.
The comparison of volatile oil extracting amount, while carrying out the distillating extracting oil in the preparation step of embodiment 1 and embodiment 5
Step distills the identical time using same amount of medicinal material, hence it is evident that it can be observed that the volatilization oil mass that embodiment 1 obtains is only real
Apply the 85% of example 5.
Stability test
Sample made from Example 3, embodiment 4 and 5 and comparative example 2, according to " Chinese medicine, natural drug stability are ground
Study carefully technological guidance's principle " carry out accelerated stability investigation.
The results are shown in Table 2 for stability test, and as seen from the experiment, the drug that preparation method of the invention obtains is waved
Hair oil stability significantly improves, and the stability of volatile oil is apparently higher than control group, the oral solution saved at a normal temperature
Middle volatile oil will not quickly disappear, and influence the curative effect of oral solution.
Table 2
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (7)
1. compound Chinese wampee leaf oral solution, which is characterized in that including following raw material: 700-900 parts of Chinese wampee leaf, wrinkled giant hyssop 40-
60 parts, 18-22 parts of peppermint, 48-52 parts of Radix Isatidis, 48-52 parts of glutinous rehmannia.
2. compound Chinese wampee leaf oral solution as described in claim 1, which is characterized in that further include parts by weight meter: 2-2.5 parts single
Syrup, 1-1.5 part potassium sorbate, 0.5-1 parts of polyoxyethylene sorbitan monoleates.
3. the preparation method of compound Chinese wampee leaf oral solution as claimed in claim 2, which is characterized in that specific step is as follows:
Step 1: the Chinese wampee leaf of above-mentioned parts by weight, wrinkled giant hyssop, peppermint distillation are extracted, the dregs of a decoction, aqueous solution and volatile oil are obtained；
Step 2: the dregs of a decoction and the Radix Isatidis of above-mentioned parts by weight, glutinous rehmannia are mixed evenly, mixture is obtained, is added into mixture
Enter the water of 2 times of its total weight, the first decocting liquid and the first residue is obtained by filtration in 80-120 DEG C of decoction 5h, then adds into the first residue
Enter the water of 2 times of its total weight, the second decocting liquid is obtained by filtration in 150-220 DEG C of decoction 30min, merges the first decocting liquid and the second decocting liquid
Total filtrate is obtained, total filtrate merges to obtain mixed liquor with aqueous solution obtained in step 1, and mixed liquor is concentrated into original volume
20%, add ethyl alcohol to make the 62-68% of wt containing amount of alcohol, refrigerate 40-56 hours, filtering obtains refrigeration liquid, by refrigeration liquid concentration
To the 35% of original volume, concentrate is obtained；
Step 3: the simple syrup of above-mentioned parts by weight, potassium sorbate are added into concentrate, 40 DEG C of heating water baths stir evenly 3 DEG C
2h is refrigerated, is filtered to get filtrate, then volatile oil, the polyoxyethylene sorbitan monoleate of above-mentioned parts by weight and water is added to 1000 weight into filtrate
Part, stir evenly, encapsulating, sterilizing to get.
4. the preparation method of compound Chinese wampee leaf oral solution as claimed in claim 3, which is characterized in that Calusena lansium before step 1
Leaf, wrinkled giant hyssop, peppermint will be pre-processed, and Chinese wampee leaf, wrinkled giant hyssop, peppermint are ground into 20-30 mesh powder, by uniformly mixed Huang
Pi Ye, wrinkled giant hyssop, peppermint powder be clipped between two layers of gauze, two layers of gauze clip is between two panels metal mesh.
5. the preparation method of compound Chinese wampee leaf oral solution as claimed in claim 3, which is characterized in that distill and extract in step 1
The steam used in volatile oil for volume fraction be 98% water vapour and volume fraction be 2% alcohol vapour mixing steam
Vapour.
6. the preparation method of compound Chinese wampee leaf oral solution as claimed in claim 3, which is characterized in that the volatile oil in step 3
Need to add stabilizer before adding, then dry using centrifugal spray dryer drying prescription, the stabilizer includes following parts by weight
Raw material: 2 parts of Divine Comedy, 2 parts of Radix Glycyrrhizae, 1 part of dandelion, 35 parts of pueraria lobata.
7. the preparation method of compound Chinese wampee leaf oral solution as claimed in claim 6, which is characterized in that the stabilizer it is specific
The preparation method comprises the following steps:
S1, the chopping of the Divine Comedy of above-mentioned parts by weight, Radix Glycyrrhizae and pueraria lobata is uniformly mixed to obtain object to be extracted；
1 part of potassium sorbate of parts by weight meter, 1 part of acetic acid and 98 parts of deionized waters are uniformly mixed and are dissolved to clarification by S2, must be extracted
Liquid；
S3, the extracting solution that 1.2 times of its total weight parts are then added into object to be extracted, are heated to 80 DEG C, extract 3h, temperature filtration
Filtrate is obtained, the dandelion of above-mentioned parts by weight, constant temperature insulated and stirred 1h, temperature filtration, filter are added when filtrate is cooled to 60 DEG C
Liquid is stabilizer.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102416067A (en) * | 2010-09-27 | 2012-04-18 | 姚昂 | Traditional Chinese medicine for preventing and treating influenza |
| CN103479721A (en) * | 2013-07-16 | 2014-01-01 | 郝振荣 | Proved prescription used for treating influenza |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102416067A (en) * | 2010-09-27 | 2012-04-18 | 姚昂 | Traditional Chinese medicine for preventing and treating influenza |
| CN103479721A (en) * | 2013-07-16 | 2014-01-01 | 郝振荣 | Proved prescription used for treating influenza |
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| 全国中草药汇编组: "《全国中草药汇编 上》", 30 September 1975, 人民卫生出版社 * |
| 张永光: "常用抗病毒中药介绍", 《养殖技术顾问》 * |
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