CN109709229A - A kind of mass spectrum discrimination method of Atlantic salmon and rainbow trout - Google Patents
A kind of mass spectrum discrimination method of Atlantic salmon and rainbow trout Download PDFInfo
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- CN109709229A CN109709229A CN201910069235.9A CN201910069235A CN109709229A CN 109709229 A CN109709229 A CN 109709229A CN 201910069235 A CN201910069235 A CN 201910069235A CN 109709229 A CN109709229 A CN 109709229A
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Abstract
The invention discloses the mass spectrum discrimination method of a kind of atlantic salmon and rainbow trout, include the following steps: the amino acid sequence for a) obtaining Atlantic salmon；B) amino acid sequence of rainbow trout is obtained；C) protein to be detected is cut into the polypeptide fragment mixture of small-molecular-weight using the enzyme solution of specificity, utilize the molecular weight and fragment information of each polypeptide in electrostatic field orbit trap high resolution mass spectrum full scan mode detection mixture, and be compared with the amino acid sequence that front obtains, qualitatively judge the feature peptide fragment that whether there is Atlantic salmon and rainbow trout；D) the qualitative feature peptide fragment for Atlantic salmon or rainbow trout is selected, multiple-reaction monitoring method is established using QQ-TOF mass spectrometry and carries out quantitative analysis.The present invention is identified using protein characteristic peptide fragment, can be achieved at the same time Atlantic salmon and rainbow trout Qualitative Identification and adulterated quantitative study, minimum detectability is up to 1%, high sensitivity, and accuracy is high, strong antijamming capability, and the true and false that can be widely applied to Atlantic salmon identifies.
Description
Technical field
The present invention relates to the mass spectrums of a kind of proteomic image detection method more particularly to a kind of atlantic salmon and rainbow trout
Discrimination method.
Background technique
The true and false and source assessment of marine product is component part important in food evaluation identification.Many fish foods are marked
Upper error label, or even there is the flesh of fish inferior, the personation flesh of fish to be labeled with the label of the high-quality flesh of fish, it is transparent that this has seriously affected market
Degree, compromises the health of consumer.A few days ago, Chinese Fishery circulation and processing association and salmon sub-commission member unit commonly through
Salmon, is set to the general designation of salmon fishes by the standard of perfection of salmon, and Atlantic salmon and rainbow trout are all one kind of salmon.This
The extensive concern of industry and the query of consumer are caused.
Although Atlantic salmon and rainbow trout belong to salmon fishes, Atlantic salmon is rare edible fishes popular in the world,
It contains 2.7% omega-fatty acid (EPA, DPA and DHA), can effectively reduce the cholesterol level of human body, to prevent the heart
The generation of vascular diseases.Antioxidant astaxanthin is more rich in Atlantic salmon body, astaxanthin easily aoxidizes at high temperature, only
The astaxanthin that eating raw just can guarantee in Atlantic salmon is not destroyed, thus eat raw Atlantic salmon be always consumer have deep love for it is edible
Method.Atlantic salmon relies on import more, at high price, and its yellowish pink difficulty distinguishes therefore, on domestic market occur with fresh water rainbow
Trout pretends to be the phenomenon that Atlantic salmon.The salmon that many consumers are thought is this kind of fish of Atlantic salmon rather than rainbow
This kind of fresh-water fishes of trout.Although fish and fresh-water fishes generally can all carry helminth, its distinguish be osmotic pressure etc. because
Element.The osmotic pressure of seawater is high, different from mankind's vivo environment, therefore helminth entrained by fish in most cases can not be
It survives for a long time in human body and completes life cycle using human body.As long as processing is proper, fish infection helminth is eaten raw
Risk is relatively low.And fresh-water fishes carrying helminth growth environment is similar with human internal environment, they can be in human body for a long time
It deposits and endangers larger." salmon " of about one third actually originates from the inlands such as Qinghai-Tibet Platean fresh water in my domestic market
Lake.Businessman is to reduce cost, is adulterated, with domestic rainbow trout pretend to be the behavior of Atlantic salmon seriously endangered consume it is strong
Health.And Atlantic salmon and fresh water rainbow trout are defined as the behavior of " salmon " again by salmon together by this Chinese Fishery association
Identify and introduces public view.
Because color and texture are more similar, human eye is difficult to differentiate for Atlantic salmon and rainbow trout.How can ensure that and disappears
Your valence salmon that expense person buys is strictly Atlantic salmon.Currently, the authentication technique of salmon has near-infrared spectrum technique
With the molecular biotechnologies such as PCR-RFLP, AFLP, DNA bar code, fluorescence real-time quantitative PCR.PCR-RFLP and AFLP examination used
Agent, which is harmful to the human body, cuts that operating procedure is extremely cumbersome, and the used time is longer.Although and fluorescence real-time quantitative PCR identifies species quickly standard
Really, but higher cost.High with salmon sales volume, the notch of import salmon (Atlantic salmon) further increases, benefit
The behavior of salmon is pretended to be also gradually to increase with fresh-water fishes such as rainbow trouts.For the right to know and health for guaranteeing consumer, having must
A kind of novel Mass Spectrometry detection method based on proteomics is wanted, determines the feature peptide fragment of Atlantic salmon and rainbow trout, is established more
Reaction monitoring method carries out qualitative and quantitative analysis.
Summary of the invention
Technical problem to be solved by the invention is to provide the mass spectrum discrimination method of a kind of atlantic salmon and rainbow trout, spirits
Sensitivity is high, and accuracy is high, and strong antijamming capability can be widely applied to Atlantic salmon adulterated confirmation and detection.
The present invention the technical solution adopted is that provides a kind of atlantic salmon and rainbow trout to solve above-mentioned technical problem
Mass spectrum discrimination method, include the following steps: a) obtain Atlantic salmon and rainbow trout amino acid sequence and preservation；B) using special
Protein to be detected is cut into the polypeptide fragment mixture of small-molecular-weight by the enzyme solution of property, utilizes electrostatic field orbit trap high-resolution
The molecular weight and fragment information of each polypeptide in mass spectrum full scan mode detection mixture, and with step a) and b) in amino acid sequence
Column are compared, and realize the identification of proteins and peptides；C) pass through basic Local Alignment Search Tool and Uniprot database pair
Than analysis, the feature peptide fragment of Atlantic salmon and rainbow trout is filtered out；D) using triple quadrupole bar mass spectrum to Atlantic salmon and rainbow trout
Feature peptide fragment is verified, and is established multiple-reaction monitoring method and carried out adulterated quantitative analysis.
The mass spectrum discrimination method of above-mentioned atlantic salmon and rainbow trout, wherein the step a) is from Protein Data Bank
In Uniport downloading obtain Atlantic salmon and rainbow trout amino acid sequence, the step b) and c) by mass spectral results combine divide
Analysis software determines the feature peptide fragment that whether there is Atlantic salmon and rainbow trout in polypeptide fragment mixture.
The mass spectrum discrimination method of above-mentioned atlantic salmon and rainbow trout, wherein the step b) using trypsase into
Row enzymatic hydrolysis, the full scan mass spectrogram that sample after enzymatic hydrolysis is obtained on HPLC-Q/Orbitrap with data dependence acquisition mode into
The qualitative confirmation of row protein；The step d) selects 5-6 ion pair to carry out the mostly anti-of mixtures of polypeptides for each feature peptide
It should monitor, complete quantification of protein detection.
The mass spectrum discrimination method of above-mentioned atlantic salmon and rainbow trout, wherein the step b) includes: b1) it weighs
Solid sample 2g after matter, the trifluoroacetic acid solution that 2mL mass percentage concentration 0.1% is added and 8mL chloroform soln are in 50
Be vortexed concussion 5min in milliliter centrifuge tube, in vortex oscillator, carries out chloroform degreasing, 3min is centrifuged at 4500r/min, remove
Supernatant repeats grease removal 2 times, is concentrated into reduced pressure at room temperature close dry；B2 it is 50mmol/L that 10mL molar concentration) is added in the sample
Trishydroxymethylaminomethane-acid salt solution, adjusting control pH value is 7.2, and be vortexed concussion 1min in vortex oscillator, 60 DEG C
1h is extracted in water-bath concussion, and 5min is centrifuged at 10000r/min, and it is spare to collect supernatant；B3) pipette 200 μ L said extracted liquid in
In the centrifuge tube of the low protein adsorption of 1.5mL, 150 μ L 500mmol/L ammonium bicarbonate solns are added, 10 μ L molar concentrations are
500mmol/L dithiothreitol (DTT) solution mixes in 75 DEG C of thermostat water baths of postposition and is incubated for 30min；B4 after) being cooled to room temperature, add
Enter 30 μ L 500mmol/L iodoacetamido amine aqueous solutions, is protected from light 30min；B5 it is 100mmol/L chlorine that 10 μ L molar concentrations, which) are added,
Change calcium solution and 50 μ L bovine trypsin solution, mixes well to be placed in 37 DEG C of waters bath with thermostatic control and digest overnight；B6 10 μ L) are added
Formic acid mixes, and terminates reaction.
The mass spectrum discrimination method of above-mentioned atlantic salmon and rainbow trout, wherein step d) the quantification of protein detection
Include: to choose different type, the chromatographic column of column length or internal diameter, records the variation of chromatographic isolation；Adjust flow rate of mobile phase, gradient or
Time parameter records the variation of separative efficiency；Column temperature and test solution temperature are controlled, is recorded for the feature peptide of Atlantic salmon and rainbow trout
The reproducibility of chromatographic retention；Adjust needle position, capillary voltage, orifice potential, each road gas flow and temperature and
Impact energy parameter；The arrangement and mass number range that protein hydrolysate generates multiple-charged ion are observed using full scan mode.
The mass spectrum discrimination method of above-mentioned atlantic salmon and rainbow trout, wherein further include collecting various different sources
Atlantic salmon and rainbow trout carry out the qualitative confirmation of protein characteristic peptide and quantitative detection, and collect the sample of separate sources, obtain system
Count result.
The mass spectrum discrimination method of above-mentioned atlantic salmon and rainbow trout, wherein if it is detected that feature in the step c)
Peptide fragment GGLGSAGPTGPR, GEIGGVGANGPSGPQGGR, IGSGLVQEAAPLVDK or
GDPGPGGPQGEPGAVGPAGITGDK, then qualitatively judge in mixture that there are Atlantic salmons；In the step c) if it is detected that
Feature peptide fragment GDPGPGGPQGEQGVVGPAGISGDK, then qualitatively judge in mixture that there are rainbow trout feature peptide fragments.
The present invention compare the prior art have it is following the utility model has the advantages that atlantic salmon provided by the invention and rainbow trout matter
Discrimination method is composed, the feature peptide fragment of qualitative determining Atlantic salmon and rainbow trout is capable of, and establishes Atlantic salmon and rainbow trout while determining
Property identification and precise measurement MRM method, high sensitivity, detection be limited to 1%；Accuracy is high, and strong antijamming capability can answer extensively
The confirmation and detection adulterated for Atlantic salmon.
Detailed description of the invention
Fig. 1 is the Mass Spectrometer Method flow diagram of Atlantic salmon of the present invention and rainbow trout feature peptide fragment；
Fig. 2 is the chromatographic mass spectrometry figure of Atlantic salmon feature peptide fragment quota ion pair of the present invention and qualitative ion pair；
Fig. 3 is the chromatographic mass spectrometry figure that rainbow trout of the present invention determines feature peptide fragment quota ion pair and qualitative ion pair.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is the Mass Spectrometer Method flow diagram of Atlantic salmon of the present invention and rainbow trout feature peptide fragment.
Referring to Figure 1, the detection method that the present invention uses is mainly Liquid Chromatography-Mass Spectrometry, mass spectrometry EFI
It is polypeptide detection techniques most reliable so far that mist, which ionizes (ESI),.The matter of atlantic salmon provided by the invention and rainbow trout
Discrimination method is composed, is included the following steps:
S1 amino acid sequence and the preservation of Atlantic salmon and rainbow trout) are obtained；
S2) protein to be detected is cut into the polypeptide fragment mixture of small-molecular-weight, benefit using the enzyme solution of specificity
With the molecular weight and fragment information of each polypeptide in electrostatic field orbit trap high resolution mass spectrum full scan mode detection mixture, and with step
Rapid S1) in amino acid sequence be compared, realize the identification of proteins and peptides；
S3) pass through basic Local Alignment Search Tool (basic local alignment search tool, Blast)
With Uniprot database comparative analysis, the feature peptide fragment of Atlantic salmon and rainbow trout is filtered out；
S4 it) is verified using feature peptide fragment of the triple quadrupole bar mass spectrum to Atlantic salmon and rainbow trout, and establishes more reactions
Monitoring method carries out adulterated quantitative analysis.Protein is cut into the method for the enzymatic hydrolysis of specificity or chemical hydrolysis by the present invention first
The polypeptide fragment mixture of small-molecular-weight, using each more in electrostatic field orbit trap high resolution mass spectrum full scan mode detection mixture
The molecular weight and fragment information of peptide, by a series of data input base of obtained polypeptide molecular weights or fragment ion, search
Corresponding known protein, realizes the identification of protein, i.e. peptide mass mapping (peptide mass
Fingerprint, PMF) or peptide fragment fragment ion identification method (peptide fragments identification, PFI).So
It combines related protein group software to pick out satisfactory feature peptide fragment according to testing result afterwards, establishes multiple-reaction monitoring
(MRM) method carries out quantitative analysis.
The restriction enzyme site of trypsase is K and R, after Atlantic salmon and rainbow trout trypsin digestion, it will obtain
GGLGSAGPTGPR, GEIGGVGANGPSGPQGGR, IGSGLVQEAAPLVDK, GDPGPGGPQGEPGAVGPAGITGDK tetra-
Mono- rainbow trout feature peptide fragment of Atlantic salmon feature peptide fragment and GDPGPGGPQGEQGVVGPAGISGDK, a total of five feature peptide fragment,
This five feature peptide fragments have different molecular weight, thus can be detected by mass spectrometry method, can be respectively used to determine the Atlantic Ocean
Salmon and rainbow trout.
The mass spectrum discrimination method of atlantic salmon provided by the invention and rainbow trout, in conjunction with high performance liquid chromatography-series connection four
Grade bar mass spectrum (QqQ) system and Skyline software, establish MRM method, the qualitative mirror of Atlantic salmon and rainbow trout can be achieved at the same time
Fixed and quantitative study.The determination of feature peptide fragment and the specific establishment process of MRM method are as follows:
(1) amino acid sequence that Atlantic salmon and rainbow trout are downloaded from Uniport database, to Atlantic salmon and rainbow trout enzyme
The mixtures of polypeptides that solution generates is scanned, and the confirmation of protein is carried out using Proteinpilot search software, determines its production
The amino acid sequence of object fragment.
(2) Atlantic salmon is determined according to the selection principle of feature peptide using Skyline software combination mass spectrum testing result
With the feature peptide fragment of rainbow trout, for the highest 5-6 ion pair of each feature peptide Response to selection, for establishing mixtures of polypeptides
MRM method, (* represents quota ion) as shown in the table, remaining daughter ion is for assisting qualitative analysis.
The mass spectrum discrimination method of atlantic salmon provided by the invention and rainbow trout is established Atlantic salmon and rainbow trout while being examined
The chromatographic mass spectrometry method of survey.
(1) optimize HPLC condition: investigating chromatography column parameter includes the influence to chromatographic isolation such as type, column length, internal diameter；It is excellent
Change the influences of the parameters to separative efficiency such as flow rate of mobile phase, gradient, time；Select suitable column temperature and test solution temperature；For big
Western salmon feature peptide fragment and rainbow trout feature peptide fragment investigate the reproducibility of its chromatographic retention.
(2) optimize ESI-QqQ condition: optimization needle position, capillary voltage, orifice potential, each road gas flow and temperature
Degree and impact energy parameter；Arrangement and the mass number model that protein hydrolysate generates multiple-charged ion are observed using full scan mode
It encloses.
(3) Atlantic salmon and rainbow trout for collecting the various places of production are carried out using the pre-treatment and chromatographic mass spectrometry method after optimization
The work of the qualitative confirmation and quantitative detection of Atlantic salmon feature peptide fragment and rainbow trout feature peptide fragment；And collect determining for separate sources sample
Property quantitative data, obtain statistical result.The indexs such as the range of linearity, sensitivity, reproducibility, the rate of recovery of investigation method.
The mass spectrum discrimination method of atlantic salmon provided by the invention and rainbow trout, specific enzymolysis process are as follows:
1) trifluoroacetic acid solution and 8mL chloroform soln of 2mL 0.1% is added in the solid sample 2g after weighing homogeneous
Be vortexed concussion 5min in 50 milliliters of centrifuge tubes, in vortex oscillator, carries out chloroform degreasing, is centrifuged 3min under 4500r/min, goes
It except supernatant, repeats grease removal 2 times, is concentrated into reduced pressure at room temperature close dry.
2) it is 50mmol/L trishydroxymethylaminomethane-acid salt solution (pH=7.2) that 10mL molar concentration is added in sample,
Be vortexed concussion 1min in vortex oscillator, and 1h is extracted in 60 DEG C of water-bath concussions, and 5min is centrifuged under 10000r/min, collects supernatant
Liquid is spare.
3) 200 μ L said extracted liquid accurately are pipetted in the centrifuge tube of the low protein adsorption of 1.5mL, 150 μ L is added
500mmol/L ammonium bicarbonate soln, 10 μ L molar concentrations are 500mmol/L dithiothreitol (DTT) solution, mix 75 DEG C of constant temperature of postposition
30min is incubated in water-bath.
4) after being cooled to room temperature, 30 μ L molar concentrations of addition are 500mmol/L iodoacetamido amine aqueous solution, are protected from light
30min；.
5) 10 μ L 100mmol/L calcium chloride solutions and 50 μ L bovine trypsin solution are added, mixes well and is placed on 37 DEG C
It is digested overnight in water bath with thermostatic control.
6) 10 μ L formic acid are added to mix, terminate reaction
7) sample after digesting acquires (data-dependent on HPLC-Q/Orbitrap with data dependence
Acquisition, DDA) the full scan mass spectrogram that obtains of mode carries out the qualitative confirmation of protein.
8) sample after digesting carries out quantification of protein detection on HPLC-QqQ in a manner of MRM.
In conclusion the mass spectrum discrimination method of atlantic salmon provided by the invention and rainbow trout, high sensitivity, by the party
Detection of adulterations of the method for rainbow trout in Atlantic salmon, minimum detectability is up to 1%.Accuracy is high, and strong antijamming capability can be wide
The general confirmation and detection adulterated applied to Atlantic salmon；The chromatography of Atlantic salmon feature peptide fragment quota ion pair and qualitative ion pair
Mass spectrogram as shown in Fig. 2, the chromatographic mass spectrometry figure of rainbow trout feature peptide fragment quota ion pair and qualitative ion pair as shown in figure 3, horizontal seat
It is designated as time (minute), ordinate is mass spectral intensities.
Although the present invention is disclosed as above with preferred embodiment, however, it is not to limit the invention, any this field skill
Art personnel, without departing from the spirit and scope of the present invention, when can make a little modification and perfect therefore of the invention protection model
It encloses to work as and subject to the definition of the claims.
Claims (7)
1. the mass spectrum discrimination method of a kind of atlantic salmon and rainbow trout, which comprises the steps of:
A) amino acid sequence and the preservation of Atlantic salmon and rainbow trout are obtained；
B) protein to be detected is cut into using the enzyme solution of specificity the polypeptide fragment mixture of small-molecular-weight, utilize electrostatic
The molecular weight and fragment information of each polypeptide in orbit trap high resolution mass spectrum full scan mode detection mixture, and with step a) and
B) amino acid sequence in is compared, and realizes the identification of proteins and peptides；
C) by basic Local Alignment Search Tool and the comparative analysis of Uniprot database, Atlantic salmon and rainbow trout are filtered out
Feature peptide fragment；
D) it is verified using feature peptide fragment of the triple quadrupole bar mass spectrum to Atlantic salmon and rainbow trout, and establishes multiple-reaction monitoring side
Method carries out adulterated quantitative analysis.
2. the mass spectrum discrimination method of atlantic salmon as described in claim 1 and rainbow trout, which is characterized in that the step a) from
Downloading obtains the amino acid sequence of Atlantic salmon and rainbow trout in Protein Data Bank Uniport, the step b) and c) passes through matter
Spectrum result binding analysis software determines the feature peptide fragment in polypeptide fragment mixture with the presence or absence of Atlantic salmon or rainbow trout.
3. the mass spectrum discrimination method of atlantic salmon as described in claim 1 and rainbow trout, which is characterized in that the step c)
It is digested using trypsase, what the sample after enzymatic hydrolysis was obtained on HPLC-Q/Orbitrap with data dependence acquisition mode
Full scan mass spectrogram carries out the qualitative confirmation of protein；The step d) selects 5-6 ion pair progress more for each feature peptide
The multiple-reaction monitoring of peptide mixer completes quantification of protein detection.
4. the mass spectrum discrimination method of atlantic salmon as claimed in claim 3 and rainbow trout, which is characterized in that the step c)
Include:
C1 trifluoroacetic acid solution and 8mL tri- that 2mL mass percentage concentration is 0.1% is added in the solid sample 2g after) weighing homogeneous
Chloromethanes solution is vortexed in vortex oscillator in 50 milliliters of centrifuge tubes and shakes 5min, chloroform degreasing is carried out, in 4500r/min
Lower centrifugation 3min removes supernatant, repeats grease removal 2 times, is concentrated into reduced pressure at room temperature close dry；
C2 it is 50mmol/L trishydroxymethylaminomethane-acid salt solution that 10mL molar concentration) is added in the sample, adjusts control pH
Value is 7.2, and be vortexed concussion 1min in vortex oscillator, and 1h is extracted in 60 DEG C of water-bath concussions, is centrifuged at 10000r/min
It is spare to collect supernatant by 5min；
C3 200 μ L said extracted liquid) are pipetted in the centrifuge tube of the low protein adsorption of 1.5mL, 150 μ L 500mmol/L carbonic acid are added
Hydrogen ammonium salt solution, 10 μ L 500mmol/L dithiothreitol (DTT) solution mix in 75 DEG C of thermostat water baths of postposition and are incubated for 30min；
C4 after) being cooled to room temperature, 30 μ L molar concentrations of addition are 500mmol/L iodoacetamido amine aqueous solution, are protected from light 30min；
C5 it is 100mmol/L calcium chloride solution and 50 μ L bovine trypsin solution that 10 μ L molar concentrations), which are added, mixes well postposition
It is digested overnight in 37 DEG C of waters bath with thermostatic control；
C6 10 μ L formic acid) are added to mix, terminate reaction.
5. identify the Mass Spectrometry detection method of Atlantic salmon and rainbow trout using protein characteristic peptide as claimed in claim 3, it is special
Sign is that step c) the quantification of protein detection includes:
Different type, the chromatographic column of column length or internal diameter are chosen, the variation of chromatographic isolation is recorded；
Flow rate of mobile phase, gradient or time parameter are adjusted, the variation of separative efficiency is recorded；
Column temperature and test solution temperature are controlled, for the reproducibility of the feature peptide of Atlantic salmon and rainbow trout record chromatographic retention；
Adjust needle position, capillary voltage, orifice potential, each road gas flow and temperature and impact energy parameter；Using complete
Scanning mode observes the arrangement and mass number range that protein hydrolysate generates multiple-charged ion.
6. the mass spectrum discrimination method of atlantic salmon as claimed in claim 3 and rainbow trout, which is characterized in that further include collecting
The Atlantic salmon of various different sources and rainbow trout carry out the qualitative confirmation of protein characteristic peptide fragment and quantitative detection, and collect different next
The sample in source obtains statistical result.
7. the mass spectrum discrimination method of atlantic salmon as claimed in claim 3 and rainbow trout, which is characterized in that the step c)
In if it is detected that feature peptide fragment GGLGSAGPTGPR, GEIGGVGANGPSGPQGGR, IGSGLVQEAAPLVDK or
GDPGPGGPQGEPGAVGPAGITGDK, then qualitatively judge in mixture that there are Atlantic salmons；In the step c) if it is detected that
Feature peptide fragment GDPGPGGPQGEQGVVGPAGISGDK, then qualitatively judge in mixture that there are rainbow trout feature peptide fragments.
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| CN114062686A (en) * | 2021-11-25 | 2022-02-18 | 青岛海关技术中心 | Method for identifying salmon by using specific peptide fragment group |
| CN115343407A (en) * | 2022-08-11 | 2022-11-15 | 中国检验检疫科学研究院 | Method for identifying iced fresh salmon and freeze-thaw salmon by applying iTRAQ technology |
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|---|---|---|---|---|
| CN110183512A (en) * | 2019-05-13 | 2019-08-30 | 大连工业大学 | A kind of preparation method of Patinopecten yessoensis dipeptides, its virtual screening method and its plural gel |
| JP2021081406A (en) * | 2019-11-20 | 2021-05-27 | 中国計量大学 | Method and system for distinguishing mixture of production areas of salmon |
| CN111323522A (en) * | 2020-04-22 | 2020-06-23 | 中国海洋大学 | Method for identifying silver carp component in food and application |
| CN111323522B (en) * | 2020-04-22 | 2022-01-04 | 中国海洋大学 | Method for identifying silver carp component in food and application |
| CN111474281A (en) * | 2020-05-27 | 2020-07-31 | 中国检验检疫科学研究院 | Method for identifying salmon species by using characteristic identification peptide fragment |
| CN111474281B (en) * | 2020-05-27 | 2022-08-05 | 中国检验检疫科学研究院 | Method for identifying salmon species by using characteristic identification peptide fragment |
| CN111678973A (en) * | 2020-07-18 | 2020-09-18 | 上海海洋大学 | Method for rapidly identifying Atlantic salmon and rainbow trout based on mass spectrometry technology |
| CN114062686A (en) * | 2021-11-25 | 2022-02-18 | 青岛海关技术中心 | Method for identifying salmon by using specific peptide fragment group |
| CN115343407A (en) * | 2022-08-11 | 2022-11-15 | 中国检验检疫科学研究院 | Method for identifying iced fresh salmon and freeze-thaw salmon by applying iTRAQ technology |
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