CN109706218A - A kind of kit measuring Remnant lipoprotein cholesterol - Google Patents
A kind of kit measuring Remnant lipoprotein cholesterol Download PDFInfo
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- CN109706218A CN109706218A CN201910175811.8A CN201910175811A CN109706218A CN 109706218 A CN109706218 A CN 109706218A CN 201910175811 A CN201910175811 A CN 201910175811A CN 109706218 A CN109706218 A CN 109706218A
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Abstract
The present invention relates to a kind of kits for measuring Remnant lipoprotein cholesterol, belong to Remnant lipoprotein cholesterol determination techniques field.Kit of the present invention includes reagent 1 and reagent 2；The reagent 1 includes: PIPES buffer, chromogenic substrate, surfactant 1 and peroxidase；The reagent 2 includes: PIPES buffer, 4-AA, phospholipase D, cholesterol esterase, cholesterol oxidase, peroxidase and surfactant 2.Kit provided by the invention is easy to operate, is suitable for clinical detection, and the reagent detection range of linearity is wide, and detection sensitivity is high, and stability is good, strong antijamming capability, has good veracity and precision.
Description
Technical field
The present invention relates to Remnant lipoprotein cholesterol determination techniques fields, and in particular to a kind of measurement remnant lipoprotein gallbladder is solid
The kit of alcohol.
Background technique
Remnant lipoprotein (Remnant-Like Particle, RLP) also known as rich in triglycerides (triglyceride,
TG) remnant lipoprotein (TRL) is chylomicron (chylomicron, CM) and very low density lipoprotein (very low
Density lipoprotein, VLDL) through lipoprotein lipase (lipoprotein lipase, LPL) hydrolysis gradually lose glycerol three
Rouge, phosphatide, aPoA (apoproteinA, apoA) and apoC, be changed into rich in cholesterol, cholesteryl ester and apoE compared with
Little particle.RLP has atharosclerosis effect, and Remnant lipoprotein cholesterol (RLP-Cholesterol, RLP-C) is horizontal
Raising is atherosclerosis and coronary heart disease (coronaryheartdisease, CHD) and atherosclerotic
The important risk factor of related metabolic disease.RLP-C concentration in atherosclerosis and is moved in blood plasma or serum
Significantly increase in the relevant disease of pulse atherosclerosis (such as type-2 diabetes mellitus, type III hyperlipidemia and Chronic renal faiure and fat patient)
Add.
The main method of detection RLP-C is immunoabsorption at present, but this method needs to carry out at separation tested sample
Reason, process is cumbersome, need to take a significant amount of time.
Summary of the invention
The purpose of the present invention is to provide a kind of kits for measuring Remnant lipoprotein cholesterol.Reagent provided by the invention
Box is not only easy to operate, is suitable for clinical detection, and the reagent detection range of linearity is wide, and detection sensitivity is high, and stability is good, resists
Interference performance is strong, has good veracity and precision.
The present invention provides a kind of kits for measuring Remnant lipoprotein cholesterol, including reagent 1 and reagent 2；
The reagent 1 includes: PIPES buffer, chromogenic substrate, surfactant 1 and peroxidase；The colour developing bottom
Object includes N- ethyl-N- (2- hydroxyl -3- sulfopropyl) -3- methylaniline；The surfactant 1 includes fatty amine and epoxy second
In alkane, the block polymer of propylene oxide, dodecyltriethanolamine sulfate, TRITON X-100 and nonylphenol polyoxyethylene ether
It is one or more；
The reagent 2 includes: PIPES buffer, 4-AA, phospholipase D, cholesterol esterase, cholesterol oxygen
Change enzyme, peroxidase and surfactant 2；The surfactant 2 includes alkyl phenol polyoxyethylene ether, the Pluronic F-127 moon
One of osmanthus acyl ether and fatty alcohol polyoxyethylene ether are a variety of.
Preferably, the concentration of the reagent 1 and the PIPES buffer in reagent 2 is separately 10~100mM,
PH value independently is 5.0~10.0.
Preferably, the mass concentration of the chromogenic substrate is 0.05~4.0g/L.
Preferably, the fatty amine and ethylene oxide, the block polymer of propylene oxide, three ethyl alcohol of dodecyl sulphate
The mass concentration of amine, TRITON X-100 and nonylphenol polyoxyethylene ether is separately 1~20g/L.
Preferably, the enzyme concentration of peroxidase independently is 5~20KU/L in the reagent 1 and reagent 2.
Preferably, the mass concentration of the 4-AA is 0.01~3g/L.
Preferably, the enzyme concentration of the phospholipase D is 3~15KU/L.
Preferably, the enzyme concentration of the cholesterol esterase is 1~10KU/L.
Preferably, the enzyme concentration of the cholesterol oxidase is 1~10KU/L.
Preferably, the alkyl phenol polyoxyethylene ether, Pluronic F-127 lauroyl ether and fatty alcohol polyoxyethylene ether
Mass concentration is separately 0.5~8g/L.
The present invention provides a kind of kits for measuring Remnant lipoprotein cholesterol.Kit provided by the invention is subsequent
Easy to operate without carrying out sample pre-treatments in use, detection quickly, is suitable for full automatic biochemical apparatus, is suitable for clinical detection,
The reagent detection range of linearity is wide, and detection sensitivity is high, and stability is good, strong antijamming capability, has good accuracy and precision
Degree.
Detailed description of the invention
Fig. 1 be the kit of embodiment 1 that provides of the embodiment of the present invention 5 with control group related coefficient comparison result figure；
Fig. 2 be the kit of embodiment 2 that provides of the embodiment of the present invention 5 with control group related coefficient comparison result figure；
Fig. 3 be the kit of embodiment 3 that provides of the embodiment of the present invention 5 with control group related coefficient comparison result figure；
Fig. 4 be the kit of embodiment 4 that provides of the embodiment of the present invention 5 with control group related coefficient comparison result figure；
Fig. 5 is the range of linearity testing result figure that the embodiment of the present invention 7 provides.
Specific embodiment
The present invention provides a kind of kits for measuring Remnant lipoprotein cholesterol, including reagent 1 and reagent 2；
The reagent 1 includes: PIPES buffer, chromogenic substrate, surfactant 1 and peroxidase；The colour developing bottom
Object includes N- ethyl-N- (2- hydroxyl -3- sulfopropyl) -3- methylaniline；The surfactant 1 includes fatty amine and epoxy second
In alkane, the block polymer of propylene oxide, dodecyltriethanolamine sulfate, TRITON X-100 and nonylphenol polyoxyethylene ether
It is one or more；
The reagent 2 includes: PIPES buffer, 4-AA, phospholipase D, cholesterol esterase, cholesterol oxygen
Change enzyme, peroxidase and surfactant 2；The surfactant 2 includes alkyl phenol polyoxyethylene ether, the Pluronic F-127 moon
One of osmanthus acyl ether and fatty alcohol polyoxyethylene ether are a variety of.
In the present invention, the concentration of the reagent 1 and the PIPES buffer in reagent 2 be separately 10~
100mM, pH value independently are 5.0~10.0.In the present invention, the concentration of the PIPES buffer is preferably 40mM, and pH value is excellent
It is selected as 7.2.In the present invention, PIPES buffer is capable of providing a suitable environment, and the enzyme material in reagent is made to keep steady
Fixed, activity will not reduce, to influence the reliability of result.The present invention is not particularly limited the source of PIPES buffer, adopts
With PIPES buffer conventional commercial product well known to those skilled in the art.
In the present invention, the chromogenic substrate preferably includes N- ethyl-N- (2- hydroxyl -3- sulfopropyl) -3- methylaniline
(TOOS), in the present invention, the mass concentration of the chromogenic substrate is 0.05~4.0g/L, preferably 0.5g/L.Institute of the present invention
Stating chromogenic substrate TOOS can make reaction sensitiveer, and accuracy is higher.In the present invention, the chromogenic substrate TOOS is preferably purchased
From Roche Holding Ag (Roche).
In the present invention, the surfactant 1 preferably includes the block polymerization of fatty amine Yu ethylene oxide, propylene oxide
Object (B-28 is preferably purchased from Kao Corp), dodecyltriethanolamine sulfate (EMAL TD, preferably purchased from flower king's strain formula meeting
Society), (NP-40 is preferably purchased from upper for TRITON X-100 (preferably be purchased from U.S. AMRESCO company) and nonylphenol polyoxyethylene ether
Extra large Aladdin biochemical technology limited liability company) one of or it is a variety of.In the present invention, described B-28, EMALTD, TRITON
The mass concentration of X-100 and NP-40 is separately 1~20g/L.In the present invention, the concentration of the B-28 be preferably 1~
5g/L, more preferably 2g/L.In the present invention, the concentration of the EMAL TD is preferably 1~5g/L, more preferably 2g/L.At this
In invention, the concentration of the TRITON X-100 is preferably 1~5g/L, more preferably 1.5g/L.In the present invention, the NP-
40 concentration is preferably 2~4g/L, more preferably 1g/L.In the present invention, the B-28, EMALTD, TRITON X-100 and
NP-40 is preferably purchased from Kao Corp.In the present invention, the surfactant 1 can inhibit to remove Remnant lipoprotein cholesterol
Cholesterol and cholesterol esterase and cholesterol oxidation enzyme reaction in addition, widely inhibit the cholesterol in sample
And keep the stabilization of system reproducibility.
In the present invention, the enzyme concentration of peroxidase independently is 5~20KU/L in the reagent 1 and reagent 2.At this
In invention, the enzyme concentration of the peroxidase is more preferably 10~20KU/L, most preferably 18KU/L.In reagent 1 of the present invention
In, the effect of the peroxidase is to eliminate interference of original hydrogen peroxide to result in sample；It is described in reagent 2
The effect of peroxidase is that life is decomposed under the action of Remnant lipoprotein cholesterol is in cholesterol esterase and cholesterol oxidase
At hydrogen peroxide, colour generation is reacted through Trinder ' s, i.e., hydrogen peroxide is deposited existing for the peroxidase and 4-AA
Under, chromogenic reaction occurs with chromogenic substrate, Remnant lipoprotein cholesterol content is gone out by its absorbance measurement.The present invention is to described
The source of peroxidase is not particularly limited, and is using conventional peroxide enzyme well known to those skilled in the art commercial product
Can, the peroxidase that such as TOYOBO Japan, Japan is spun.
In the present invention, the mass concentration of the 4-AA is 0.01~3g/L.In the present invention, the 4-
The mass concentration of amino-antipyrine is more preferably 0.2g/L.In the present invention, the hydrogen peroxide that above-mentioned reaction generates is in 4- ammonia
In the presence of base antipyrine and peroxidase, chromogenic reaction occurs with chromogenic substrate, is detected so as to quantitative
The amount of Remnant lipoprotein cholesterol.The present invention does not have special restriction to the source of the 4-AA, using ability
Conventional commercial 4-AA known to field technique personnel, the 4- sold such as Sinopharm Chemical Reagent Co., Ltd.
Amino-antipyrine.
In the present invention, the enzyme concentration of the phospholipase D is 3~15KU/L.In the present invention, the enzyme of the phospholipase D
Concentration is preferably 6~12KU/L, more preferably 10KU/L.In the present invention, the effect of the phospholipase D is and surfactant
Remnant lipoprotein cholesterol, is changed into the remnant-like lipoprotein of solubilised state by 2 synergistic effects.The present invention is to the phospholipase D
Source does not have special restriction, using the conventional commercial product of phospholipase D well known to those skilled in the art, such as Japan
The phospholipase D that ASAHI Asahi Chemical Industry sells.
In the present invention, the enzyme concentration of the cholesterol esterase is 1~10KU/L.In the present invention, the cholesteryl ester
The enzyme concentration of enzyme is preferably 2~5KU/L, more preferably 2KU/L.It is special that the present invention does not have the source of the cholesterol esterase
It limits, using the conventional commercial product of cholesterol esterase well known to those skilled in the art, such as ASAHI Asahi Chemical Industry, Japan is public
Take charge of the cholesterol esterase sold.
In the present invention, the enzyme concentration of the cholesterol oxidase is 1~10KU/L.In the present invention, the cholesterol
The enzyme concentration of oxidizing ferment is preferably 2~8KU/L, more preferably 5KU/L.The present invention does not have the source of the cholesterol oxidase
Special restriction, using the conventional commercial product of cholesterol oxidase well known to those skilled in the art, such as Japan ASAHI
The cholesterol oxidase that Asahi Kasei Corporation sells.
In the present invention, the surfactant 2 includes alkyl phenol polyoxyethylene ether (NP-10, preferably purchased from flower king's strain formula
Commercial firm), Pluronic F-127 lauroyl ether (Brij-35, preferably be purchased from Shanghai Ru Ji Biotechnology Co., Ltd) and poly alkyl alcohol
One of ethylene oxide ether (B-66 is preferably purchased from Kao Corp) is a variety of.In the present invention, described NP-10, Brij-
The mass concentration of 35 and B66 is separately 0.5~8g/L.In the present invention, the concentration of the NP-10 is preferably 2~5g/
L, more preferably 3g/L.In the present invention, the concentration of the Brij-35 is preferably 5~8g/L, more preferably 6g/L.In this hair
In bright, the concentration of the B66 is preferably 2~5g/L, more preferably 4g/L.In the present invention, described NP-10, Brij-35 and
B66 is preferably purchased from Kao Corp.In the present invention, the surfactant 2 being capable of targetedly proteolipid protein remnant
Cholesterol, formed stable compound, the compound have good water solubility, thus in the environment of offer with cholesterol esterase
And cholesterol oxidation enzyme reaction, realize the release and modification of Remnant lipoprotein cholesterol.
In the present invention, the detection sample of the kit includes: serum, blood plasma.
In the present invention, the application method of the kit are as follows: 5ul sample and 240ul reagent 1 mix, and set 37 DEG C and are incubated for 5
Minute, measure absorbance A 1；80ul reagent 2 is added, 37 DEG C is set and is incubated for 5 minutes, measure absorbance A 2.△ A=A2-A1.Meter
It calculates:
In the present invention, the model (source) of the biochemical instruments is preferably 7100 automatic clinical chemistry analyzer of Hitachi.At this
In invention, the parameter setting of the biochemical instruments is preferred are as follows: wavelength: 600nm (dominant wavelength)/700nm (commplementary wave length), temperature: 37 DEG C,
Reagent 1:240 μ L, reagent 2:80 μ L, sample: 5 μ L.
1) surfactant in reagent and phospholipase D synergistic effect, selective modification remnant-like lipoprotein (RLPs),
It is dissolved in it in reaction system, forms the remnant-like lipoprotein of solubilised state, while shielding other lipoprotein insoluble in reaction system
In.2) cholesteryl ester in the remnant-like lipoprotein of solubilised state (RLP-c ester) forms free gallbladder through cholesteryl ester enzyme hydrolysis
Sterol.3) free cholesterol generated produces cholestenone and hydrogen peroxide under the action of cholesterol oxidase, and passes through
Trinder reaction generates the colour developing of color source.In entire reaction process, through surfactant and phospholipase D, cholesterol esterase etc. is a variety of
The collective effect of enzyme excludes other lipoprotein in addition to RLPs, and the reagent detection range of linearity is wide, and detection sensitivity is high, surely
Qualitative good, strong antijamming capability has good veracity and precision.
Combined with specific embodiments below to it is of the present invention it is a kind of measure Remnant lipoprotein cholesterol kit do into
The detailed introduction of one step, technical solution of the present invention include but is not limited to following embodiment.Embodiment 1
Reagent 1:
PIPES buffer (pH7.2) 40mM
TOOS 0.5g/L
Surfactant 1
B-28 5g/L
T80 8g/L
Peroxidase 20KU/L
Reagent 2:
Surfactant 2
Brij-35 1g/L
B66 2g/L
Embodiment 2:
Reagent 1:
PIPES buffer (pH7.2) 40mM
TOOS 0.5g/L
Surfactant 1
T80 10g/L
NP-40 5g/L
Peroxidase 20KU/L
Reagent 2:
Surfactant 2
NP-10 4g/L
B66 5g/L
Embodiment 3:
Reagent 1:
PIPES buffer (pH7.2) 40mM
TOOS 0.5g/L
Surfactant 1
B-28 2g/L
NP-40 1g/L
Peroxidase 20KU/L
Reagent 2:
Surfactant 2
NP-10 3g/L
Brij-35 6g/L
Embodiment 4:
Reagent 1:
PIPES buffer (pH7.2) 40mM
TOOS 0.5g/L
Surfactant 1
EMALTD 7g/L
T80 4g/L
Peroxidase 20KU/L
Reagent 2:
Embodiment 5
By Examples 1 to 4, ((immunoprecipitation separates Remnant lipoprotein cholesterol (RLP-C) assay kit with control group
Method) (Shanghai Beijia Biochemical Reagent Co., Ltd.)) be compared, comparison result is as shown in figures 1-4.Comparison result shows to implement
Example 1~4 has good correlation with control group, wherein embodiment 3 and control group degree of correlation highest.Related coefficient is contrasted
The results are shown in Table 1.
1 Examples 1 to 4 of table and control group related coefficient deck watch
| Embodiment and control group | Coefficient R |
| 1 | 0.9996 |
| 2 | 0.9993 |
| 3 | 0.9998 |
| 4 | 0.9995 |
Embodiment 6
RLP-C detection parameters are as described in Table 2.
The full-automatic parameter setting table of 2 RLP-C of table
The method of precision test: clinical samples or quality controlled serum test kit are used, retest 10 times, calculates measurement
The average value of valueWith standard deviation (s).The coefficient of variation (CV) is calculated by formula (2),
Precision (10 Quality Controls of retest (two low concentration, high concentration horizontal Quality Controls)) test result such as 3 He of table
Shown in table 4:
3 low concentration precision test result of table
4 high concentration precision test result of table
3 batches of reagents are prepared, Quality Control value is measured, calculate difference between batch: selecting a clinical samples or quality controlled serum, not with three batches
With lot number kit respectively measure calculate separately three times every batch of 3 times detection mean values, by following calculation relative deviation (R), as a result
It is as shown in table 5:
5 difference between batch measurement result (unit mg/dl) of table
10 Quality Control levels 1 (low concentration) of retest, CV value are 7.05%；10 Quality Control levels 2 of retest are (highly concentrated
Degree), CV value is 2.06%.It proves that the detection reagent precision is high, there is good repeatability.
(low concentration) difference between batch of Quality Control level 1 is 7.24%, and (high concentration) difference between batch of Quality Control level 2 is 2.05%.It proves
Detection reagent difference between batch is small, and stability is good between batch, favorable reproducibility.
Embodiment 7
Range of linearity measurement result is as shown in table 6 and Fig. 5.
6 range of linearity measurement result of table
According to Fig. 5 and table 6 it is found that the range of linearity is wide, linearly dependent coefficient 0.9971, range 1mg/dl-80mg/dl,
The area requirement of clinical detection can be completely covered.
Embodiment 8
Sensitivity for analysis
With known concentration or active sample test kit, it is recorded in kit and provides that the absorbance generated under parameter changes
Become, is scaled the absorbance change rate (△ A/min) of n (undetermined) unit
7 sensitivity analysis result of table
Absorbance change rate (△ A/min) is higher, and the sensitivity of reaction is higher.When reagent test 10mg/dl measured object, inhale
Luminosity difference (△ A) is 0.0482, and reaction sensitivity is high.
Embodiment 9
Interference test
With the ascorbic acid of the various concentration diluted, hemoglobin, bilirubin and triglycerides respectively with containing RLP-C compared with
The serum of high concentration (70mg/dl) carries out 1:9 mixing (first concentration uses physiological saline) and does recovery test, with the height of the rate of recovery
The degree of low judgement interference.Chaff interferent test result is as shown in table 8.
8 chaff interferent test result of table
As shown in Table 8, ascorbic acid≤50mg/dl, hemoglobin≤500mg/dl, bilirubin≤20mg/dl, glycerol three
To measurement RLP-C without significantly interfering with when ester≤3000mg/dl.
Anti-interference ability is an important performance indexes of detection reagent, ascorbic acid, hemoglobin, gallbladder in serum sample
Red pigment, triglyceride concentration will affect the result of detection reagent measurement.It is above-mentioned experiments have shown that detection reagent provided by the present invention
In ascorbic acid≤50mg/dl, hemoglobin≤500mg/dl, bilirubin≤20mg/dl, triglycerides≤3000mg/dl is right
RLP-C measurement result is without significantly interfering with.Provided chaff interferent range is much higher than Abnormal Serum sample, keeps testing result more acurrate
Reliably.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of kit for measuring Remnant lipoprotein cholesterol, which is characterized in that including reagent 1 and reagent 2；
The reagent 1 includes: PIPES buffer, chromogenic substrate, surfactant 1 and peroxidase；The chromogenic substrate packet
Include N- ethyl-N- (2- hydroxyl -3- sulfopropyl) -3- methylaniline；The surfactant 1 include fatty amine and ethylene oxide,
In the block polymer of propylene oxide, dodecyltriethanolamine sulfate, TRITON X-100 and nonylphenol polyoxyethylene ether
It is one or more；
The reagent 2 include: PIPES buffer, 4-AA, phospholipase D, cholesterol esterase, cholesterol oxidase,
Peroxidase and surfactant 2；The surfactant 2 includes alkyl phenol polyoxyethylene ether, Pluronic F-127 lauroyl
One of ether and fatty alcohol polyoxyethylene ether are a variety of.
2. kit according to claim 1, which is characterized in that PIPES buffer in the reagent 1 and reagent 2
Concentration is separately 10~100mM, and pH value independently is 5.0~10.0.
3. kit according to claim 1, which is characterized in that the mass concentration of the chromogenic substrate be 0.05~
4.0g/L。
4. kit according to claim 1, which is characterized in that the fatty amine and ethylene oxide, propylene oxide it is embedding
Section polymer, dodecyltriethanolamine sulfate, the mass concentration difference of TRITON X-100 and nonylphenol polyoxyethylene ether are only
It is on the spot 1~20g/L.
5. kit according to claim 1, which is characterized in that the enzyme of peroxidase is dense in the reagent 1 and reagent 2
Degree independently is 5~20KU/L.
6. kit according to claim 1, which is characterized in that the mass concentration of the 4-AA is 0.01
~3g/L.
7. kit according to claim 1, which is characterized in that the enzyme concentration of the phospholipase D is 3~15KU/L.
8. kit according to claim 1, which is characterized in that the enzyme concentration of the cholesterol esterase is 1~10KU/L.
9. kit according to claim 1, which is characterized in that the enzyme concentration of the cholesterol oxidase is 1~10KU/
L。
10. kit according to claim 1, which is characterized in that the alkyl phenol polyoxyethylene ether, the Pluronic F-127 moon
The mass concentration of osmanthus acyl ether and fatty alcohol polyoxyethylene ether is separately 0.5~8g/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910175811.8A CN109706218A (en) | 2019-03-08 | 2019-03-08 | A kind of kit measuring Remnant lipoprotein cholesterol |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910175811.8A CN109706218A (en) | 2019-03-08 | 2019-03-08 | A kind of kit measuring Remnant lipoprotein cholesterol |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023085323A1 (en) * | 2021-11-12 | 2023-05-19 | 東洋紡株式会社 | Method for reducing measurement errors due to hemolyzed hemoglobin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1132482A2 (en) * | 2000-02-28 | 2001-09-12 | Kyowa Medex Co., Ltd. | Method and reagent for determination of cholesterol in remnant-like lipoprotein particles |
| CN1914333A (en) * | 2004-01-29 | 2007-02-14 | 协和梅迪克斯株式会社 | Method, reagent, and kit for determining cholesterol in very-low-density lipoprotein remnant (vldl remnant) |
-
2019
- 2019-03-08 CN CN201910175811.8A patent/CN109706218A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1132482A2 (en) * | 2000-02-28 | 2001-09-12 | Kyowa Medex Co., Ltd. | Method and reagent for determination of cholesterol in remnant-like lipoprotein particles |
| CN1914333A (en) * | 2004-01-29 | 2007-02-14 | 协和梅迪克斯株式会社 | Method, reagent, and kit for determining cholesterol in very-low-density lipoprotein remnant (vldl remnant) |
Non-Patent Citations (1)
| Title |
|---|
| 木和: "也门乳糜泻病人与不育症的关系研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023085323A1 (en) * | 2021-11-12 | 2023-05-19 | 東洋紡株式会社 | Method for reducing measurement errors due to hemolyzed hemoglobin |
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Application publication date: 20190503 |