CN109700899A - A kind of Rhizoma Belamcandae skullcapflavone extract and its preparation method and application - Google Patents
A kind of Rhizoma Belamcandae skullcapflavone extract and its preparation method and application Download PDFInfo
- Publication number
- CN109700899A CN109700899A CN201910186239.5A CN201910186239A CN109700899A CN 109700899 A CN109700899 A CN 109700899A CN 201910186239 A CN201910186239 A CN 201910186239A CN 109700899 A CN109700899 A CN 109700899A
- Authority
- CN
- China
- Prior art keywords
- extract
- rhizoma belamcandae
- skullcapflavone
- preparation
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a kind of Rhizoma Belamcandae skullcapflavone extracts, it is extracted with water using Rhizoma Belamcandae and Baical Skullcap Root as raw material, and extracting solution is isolated and purified, filtering, dry manufactured extract.The anti-inflammatory of Rhizoma Belamcandae skullcapflavone extract of the present invention, analgesia, antitussive action are also better than blackberry lily chromocor extract, the blackberry lily skullcapflavone extract of same crude drug amount better than individual Rhizoma Belamcandae chromocor extract, skullcapflavone extract and Rhizoma Belamcandae, the physical mixture of skullcapflavone extract other ratios.The present invention also provides a kind of preparation methods of Rhizoma Belamcandae skullcapflavone extract, have stronger practical application value, use easy to spread.
Description
Technical field
Present invention relates particularly to a kind of Rhizoma Belamcandae skullcapflavone extracts and its preparation method and application.
Background technique
Radix scutellariae is the dry root of Lamiaceae plant radix scutellariae Scutellariabaicalensis Georgi, and property bitter taste is cold, tool
Have the effect of heat-clearing and damp-drying drug, purging fire for removing toxin, be used for damp-warm syndrome, summer-heat and damp, chest tightness and emesis, damp and hot feeling of fullness, dysentery, jaundice, cough with lung heat,
Hyperpyrexia polydipsia, blood-head tells nosebleed etc..Blackberry lily is the dry rhizome of irides blackberry lily Belamcandachinensis (L) DC.,
Property bitter taste it is cold, there is the effect of clearing heat and detoxicating, dissolving phlegm, relieving sore-throat, be used for the pent-up of heat toxin convulsive seizure due to phlegm-fire, abscess of throat, accumulation and obstruction of sputum, cough
Asthma etc..
Modern research shows that radix scutellariae main active is based on scutelloside, wogonoside, baicalein, wogonin
Flavones ingredient, and blackberry lily main active be with belamcandin, iridin, blackberry lily aglycon, Tectorigenin and secondary open country
Osajin chemical component based on tectorigenin.Blackberry lily, radix scutellariae main active be flavone compound, main medicine
The effects of reason activity is anti-inflammatory, antibacterial, antiviral, antipyretic, analgesia, cough-relieving, is clinically used to acute and chronic tonsillitis, pharynx
The treatment of the diseases such as laryngitis and the infection of the upper respiratory tract.
Radix scutellariae blackberry lily soup is recorded in " doctor's paper money class is compiled " 12 (throat door sides) of volume, is by two taste Chinese medicine group of radix scutellariae and blackberry lily
At for treating stomach two through smelling as of rotten fish in larynx caused by heat toxin.It has been reported that prepared by radix scutellariae blackberry lily soup Amberlyst process
Sulfonamide perlingual tablet is penetrated, can be used for acpuei pharyngitis and acute episode of chronic pharyngitis phase, there are also to flavones ingredient fingerprint in radix scutellariae blackberry lily soup
Map carried out research and radix scutellariae blackberry lily soup ingredient ongoing change rule did Primary Study, and to radix scutellariae blackberry lily Tang Zhongqian
Layer paper factor A and irisflorentin did assay.A kind of patent (101185707 B of CN) anti-inflammatory antiviral drug group
It closes object, preparation method and application: disclosing a kind of anti-inflammatory antiviral drug combination, be Scullcap total-flavonoid and general flavone from blackberry lily
As active constituent, curative effect is better than exclusive use Scullcap total-flavonoid or general flavone from blackberry lily.
Rhizoma Belamcandae is the dry rhizome of irides iris Iris tectorum Maxim., and property bitter taste is cold, there is heat-clearing
Removing toxic substances, eliminating the phlegm, relieving sore-throat effect.For heat toxin convulsive seizure due to phlegm-fire pent-up, abscess of throat, accumulation and obstruction of sputum, cough and asthma.Patent (CN
100448450C) the purposes of iris extractive of general flavone discloses Rhizoma Belamcandae extractive of general flavone and is preparing anti-inflammatory antiviral agent
Purposes in object.Rhizoma Belamcandae flavone capsule (national drug standard Z20140008) is the hard capsule of Rhizoma Belamcandae chromocor extract preparation,
For treating abscess of throat, the hoarseness, cough that wind-heat syndrome of acute pharyngitis and slight acute simple laryngitis wind-heat syndrome occur
Deng.
At present do not have also Rhizoma Belamcandae, radix scutellariae compatibility pharmacological component and its for treat acute and chronic pharyngitis and on exhale
Inhale the report of road infectious disease.
Summary of the invention
To solve the above problems, the present invention provides a kind of Rhizoma Belamcandae skullcapflavone extract, it is with Rhizoma Belamcandae and Huang
A kind of reed mentioned in ancient books is raw material, is extracted with water, and extracting solution is isolated and purified, filtering, dry manufactured extract
Further, the mass ratio of the Rhizoma Belamcandae and radix scutellariae is 1:(0.1~9).
Further, the mass ratio of the Rhizoma Belamcandae and radix scutellariae is 1:1.
The present invention also provides a kind of preparation methods of Rhizoma Belamcandae skullcapflavone extract, it includes the following steps:
Medicinal material is weighed according to the proportion, grinds as smalls, is extracted with water, is filtered, and extracting solution is splined on large pore resin absorption column, first
It is eluted afterwards with water and alcohol, collects alcohol eluen, filtering, filtrate decompression is concentrated to give medicinal extract, and medicinal extract is dried under reduced pressure to get Rhizoma Belamcandae Huang
A kind of reed mentioned in ancient books chromocor extract.
Further, it is described be extracted with water be with water boiling and extraction 2~3 times of 10~30 times of v/w medicinal material amounts, every time 1~
2h；Described is successively first with the water elution of 1~3 times of v/w amount of resin, then with the 60 of 5~7 times of v/w amount of resin with water and alcohol elution
~100% ethanol elution.
Further, described be extracted with water is each 1h with water boiling and extraction 3 times of 20 times of v/w medicinal material amounts；The elder generation
It is afterwards first with the water elution of 2 times of v/w amount of resin, then the ethanol elution with 6 times of v/w amount of resin with water and ethanol elution.
Further, the macroporous absorbent resin is D101Macroporous absorbent resin, D101The dosage of macroporous absorbent resin is medicine
3 times of material.
Further, the D101Macroporous absorbent resin needs to use 8~12h of 5%NaOH alcohol solution dipping, is allowed to dry, and uses
Water displacement wash is allowed to dry to efflux pH value 7, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with water displacement wash to efflux
PH value 7.
Further, the temperature being dried under reduced pressure is 60 DEG C.
The present invention also provides a kind of Rhizoma Belamcandae skullcapflavone extracts to prepare the purposes in anti-inflammatory drug.
The present invention also provides a kind of purposes of Rhizoma Belamcandae skullcapflavone extract in preparation analgesic drug.
The present invention also provides a kind of purposes of Rhizoma Belamcandae skullcapflavone extract in the drug of preparation cough-relieving.
The present invention finally provides a kind of preparation containing Rhizoma Belamcandae skullcapflavone extract, it is yellow with Rhizoma Belamcandae radix scutellariae
Ketone extract is effective component, in addition the preparation that pharmaceutically acceptable auxiliary material is prepared.
Further, the preparation is oral preparation or injection.
Different pharmaceutical preparations is made plus pharmaceutically acceptable carrier in Rhizoma Belamcandae skullcapflavone extract of the present invention,
Injection has freeze-dried powder, tablet, injection, and oral preparation has powder, granule, capsule, pill, oral administration solution, collunarium
Agent, spray etc., wherein tablet includes: common compressed tablets, chewable tablets, effervescent tablet, multilayer tablet, sustained release tablets, controlled release tablet, coating
Piece, dispersible tablet, buccal tablet, sublingual tablet etc.；Pill includes dripping pill, the dripping pill containing change, pellet etc..Capsule includes: hard capsule, soft
Capsule, enteric capsule, Duracaps, micro-capsule etc..
Rhizoma Belamcandae skullcapflavone extract of the present invention, anti-inflammatory, analgesia, antitussive action are better than individual Rhizoma Belamcandae extracting flavonoids
The physical mixture of object, skullcapflavone extract and Rhizoma Belamcandae, the various ratios of skullcapflavone extract, is also better than same crude drug amount
Blackberry lily chromocor extract, blackberry lily skullcapflavone extract.
The present invention, which tests, to be proved, when Rhizoma Belamcandae and radix scutellariae compatibility, obtained must measuring for chromocor extract is apparently higher than same amount
Must measure when Rhizoma Belamcandae or radix scutellariae individually extract, especially when Rhizoma Belamcandae and radix scutellariae are with 1:1 ratio compatibility, since radix scutellariae is to river
Blackberry lily has hydrotropy solubilization, so that belamcandin, scutelloside rate of transform highest in extract, and extract must measure maximum.This hair
Bright Rhizoma Belamcandae skullcapflavone extract and preparation method thereof has stronger practical application value, use easy to spread.
Obviously, above content according to the present invention is not being departed from according to the ordinary technical knowledge and customary means of this field
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The specific embodiment of form by the following examples remakes further specifically above content of the invention
It is bright.But the range that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to example below.It is all to be based on above content of the present invention
The technology realized all belongs to the scope of the present invention.
Detailed description of the invention
Fig. 1 Rhizoma Belamcandae radix scutellariae brass extract ultra-violet absorption spectrum
Fig. 2 Rhizoma Belamcandae skullcapflavone extract (Rhizoma Belamcandae: radix scutellariae 1:1) identification (is respectively as follows: Rhizoma Belamcandae radix scutellariae from left to right
Extract, Rhizoma Belamcandae control medicinal material, radix scutellariae control medicinal material, belamcandin, scutelloside)
Fig. 3 Rhizoma Belamcandae skullcapflavone extract (Rhizoma Belamcandae: radix scutellariae 1:1) identification (is respectively as follows: Rhizoma Belamcandae radix scutellariae from left to right
Extract, Rhizoma Belamcandae control medicinal material, radix scutellariae control medicinal material, blackberry lily aglycon, baicalein)
Fig. 4 compare feature map (peak 2 (S): belamcandin, peak 3: iristectorin B, peak 5: iristectorin A, peak 6: wild flag
Glycosides, peak 7: peak 8: scutelloside removes first wogonoside, peak 11: qroxylin A glycosides, peak 12: wogonoside, peak 13: blackberry lily aglycon,
Peak 14: iristectorigenin B, peak 15: iristectorigenin A, Tectorigenin, peak 16: baicalein, peak 18: wogonin)
Specific embodiment
Embodiment 1, the preparation of Rhizoma Belamcandae skullcapflavone extract are 1.
Rhizoma Belamcandae coarse powder 100g, radix scutellariae coarse powder 900g are taken, is added in 20 times of boiling water and decocts extraction 3 times, 1 hour every time, mistake
Filter, combined extract, extracting solution are splined on processed (that is: D101Macroporous absorbent resin 5%NaOH alcohol solution dipping 8
~12h, is allowed to dry, and with water displacement wash to efflux pH value 7, is allowed to dry, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with stream
Water washing to efflux pH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with 2 times of amount of resin
Water washing resin column collect ethanol eluate then with 70% ethanol elution of 6 times of amount of resin, filtering, filtrate decompression recycling is molten
Agent obtains medicinal extract, 1. 60 DEG C of medicinal extract are dried under reduced pressure to get Rhizoma Belamcandae skullcapflavone extract.
1. Rhizoma Belamcandae skullcapflavone extract is the dry loose powder of brown color, the amount of obtaining 207.8g, lot number 180401.
Embodiment 2, the preparation of Rhizoma Belamcandae skullcapflavone extract are 2.
Rhizoma Belamcandae coarse powder 300g, radix scutellariae coarse powder 700g are taken, is added in 20 times of boiling water and decocts extraction 3 times, 1 hour every time, mistake
Filter, combined extract, extracting solution are splined on processed (that is: D101Macroporous absorbent resin 5%NaOH alcohol solution dipping 8
~12h, is allowed to dry, and with water displacement wash to efflux pH value 7, is allowed to dry, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with stream
Water washing to efflux pH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with 2 times of amount of resin
Water washing resin column collect ethanol eluate then with 70% ethanol elution of 6 times of amount of resin, filtering, filtrate decompression recycling is molten
Agent obtains medicinal extract, 2. 60 DEG C of medicinal extract are dried under reduced pressure to get Rhizoma Belamcandae skullcapflavone extract.
2. Rhizoma Belamcandae skullcapflavone extract is the dry loose powder of brown color, the amount of obtaining 208.5g, lot number 180402.
Embodiment 3, the preparation of Rhizoma Belamcandae skullcapflavone extract are 3.
Rhizoma Belamcandae coarse powder 500g, radix scutellariae coarse powder 500g are taken, is added in 20 times of boiling water and decocts extraction 3 times, 1 hour every time, mistake
Filter, combined extract, extracting solution are splined on processed (that is: D101Macroporous absorbent resin 5%NaOH alcohol solution dipping 8
~12h, is allowed to dry, and with water displacement wash to efflux pH value 7, is allowed to dry, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with stream
Water washing to efflux pH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with 2 times of amount of resin
Water washing resin column collect ethanol eluate then with 70% ethanol elution of 6 times of amount of resin, filtering, filtrate decompression recycling is molten
Agent obtains medicinal extract, 3. 60 DEG C of medicinal extract are dried under reduced pressure to get Rhizoma Belamcandae skullcapflavone extract.
3. Rhizoma Belamcandae skullcapflavone extract is the dry loose powder of brown color, the amount of obtaining 212.3g, lot number 180403.
4. 4 Rhizoma Belamcandae skullcapflavone extract of embodiment is prepared
Rhizoma Belamcandae coarse powder 700g, radix scutellariae coarse powder 300g are taken, is added in 20 times of boiling water and decocts extraction 3 times, 1 hour every time, mistake
Filter, combined extract, extracting solution are splined on processed (that is: D101Macroporous absorbent resin 5%NaOH alcohol solution dipping 8
~12h, is allowed to dry, and with water displacement wash to efflux pH value 7, is allowed to dry, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with stream
Water washing to efflux pH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with 2 times of amount of resin
Water washing resin column collect ethanol eluate then with 70% ethanol elution of 6 times of amount of resin, filtering, filtrate decompression recycling is molten
Agent obtains medicinal extract, 4. 60 DEG C of medicinal extract are dried under reduced pressure to get Rhizoma Belamcandae skullcapflavone extract.
4. Rhizoma Belamcandae skullcapflavone extract is the dry loose powder of brown color, the amount of obtaining 203.7g, lot number 180404.
5. 5 Rhizoma Belamcandae skullcapflavone extract of embodiment is prepared
Rhizoma Belamcandae coarse powder 900g, radix scutellariae coarse powder 100g are taken, is added in 20 times of boiling water and decocts extraction 3 times, 1 hour every time, mistake
Filter, combined extract, extracting solution are splined on processed (that is: D101Macroporous absorbent resin 5%NaOH alcohol solution dipping 8
~12h, is allowed to dry, and with water displacement wash to efflux pH value 7, is allowed to dry, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with stream
Water washing to efflux pH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with 2 times of amount of resin
Water washing resin column collect ethanol eluate then with 70% ethanol elution of 6 times of amount of resin, filtering, filtrate decompression recycling is molten
Agent obtains medicinal extract, 5. 60 DEG C of medicinal extract are dried under reduced pressure to get Rhizoma Belamcandae skullcapflavone extract.
5. Rhizoma Belamcandae skullcapflavone extract is the dry loose powder of brown color, the amount of obtaining 200.2g, lot number 180405.
Beneficial effects of the present invention are further illustrated below by way of test example.
The preparation of 1 Rhizoma Belamcandae chromocor extract of test example
Rhizoma Belamcandae coarse powder 1kg is taken, is added in 20 times of boiling water to decoct and extract 3 times, 1 hour every time, filter, combined extract,
Extracting solution is splined on processed (that is: D101Macroporous absorbent resin 5%NaOH 8~12h of alcohol solution dipping, is allowed to dry, and uses
Water displacement wash is allowed to dry to efflux pH value 7, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with water displacement wash to outflow
Liquid pH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with the water washing resin of 2 times of amount of resin
Column, then with 70% ethanol elution of 6 times of amount of resin, ethanol eluate, filtering are collected, filtrate decompression recycling design obtains medicinal extract, soaks
60 DEG C of cream are dried under reduced pressure to get Rhizoma Belamcandae chromocor extract.
Rhizoma Belamcandae chromocor extract is the dry loose powder of yellowish-brown, the amount of obtaining 175.3g, lot number 180301.
The preparation of 2 skullcapflavone extract of test example
Radix scutellariae coarse powder 1kg is taken, is added in 20 times of boiling water to decoct and extract 3 times, 1 hour every time, filtering, combined extract mentioned
Liquid is taken to be splined on processed (that is: D101Macroporous absorbent resin 5%NaOH 8~12h of alcohol solution dipping, is allowed to dry, with stream
Water washing is allowed to dry to efflux pH value 7, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with water displacement wash to efflux
PH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with the water washing resin column of 2 times of amount of resin,
Again with 70% ethanol elution of 6 times of amount of resin, ethanol eluate, filtering are collected, filtrate decompression recycling design obtains medicinal extract, medicinal extract 60
It DEG C is dried under reduced pressure to get skullcapflavone extract.
Skullcapflavone extract is the dry loose powder of brown color, the amount of obtaining 185.6g, lot number 180302.
The preparation of 3 blackberry lily chromocor extract of test example
Blackberry lily coarse powder 1kg is taken, is added in 20 times of boiling water to decoct and extract 3 times, 1 hour every time, filtering, combined extract mentioned
Liquid is taken to be splined on processed (that is: D101Macroporous absorbent resin 5%NaOH 8~12h of alcohol solution dipping, is allowed to dry, with stream
Water washing is allowed to dry to efflux pH value 7, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, with water displacement wash to efflux
PH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with the water washing resin column of 2 times of amount of resin,
Again with 70% ethanol elution of 6 times of amount of resin, ethanol eluate, filtering are collected, filtrate decompression recycling design obtains medicinal extract, medicinal extract 60
It DEG C is dried under reduced pressure to get blackberry lily chromocor extract.
Blackberry lily chromocor extract is the dry loose powder of yellowish-brown, the amount of obtaining 60.5g, lot number 180303.
4 blackberry lily skullcapflavone extract (blackberry lily: radix scutellariae 1:1) of test example
Blackberry lily coarse powder 500g, radix scutellariae coarse powder 500g are taken, is added in 20 times of boiling water to decoct and extract 3 times, 1 hour every time, filter,
Combined extract, extracting solution are splined on processed (that is: D101Macroporous absorbent resin with 5%NaOH alcohol solution dipping 8~
12h is allowed to dry, and with water displacement wash to efflux pH value 7, is allowed to dry, then impregnates 8~12h with 5% hydrochloric acid solution, is allowed to dry, is used flowing water
It washs to efflux pH value 7) D101Large pore resin absorption column (amount of resin: medicinal material amount 3:1, W/W), with 2 times of amount of resin
Water washing resin column, then with 70% ethanol elution of 6 times of amount of resin, collect ethanol eluate, filtering, filtrate decompression recycling design
Medicinal extract is obtained, 60 DEG C of medicinal extract are dried under reduced pressure to get blackberry lily skullcapflavone extract (blackberry lily: radix scutellariae 1:1).
Blackberry lily skullcapflavone extract is the dry loose powder of brown color, the amount of obtaining 125g, lot number 180304.
Comparing embodiment 1~5 and test example 1~4, it can be deduced that: when Rhizoma Belamcandae and radix scutellariae compatibility, decocting liquid passes through D101
The extract that purification with macroreticular resin technique obtains must be measured, and be significantly higher than independent same amount with Rhizoma Belamcandae, blackberry lily, radix scutellariae, or penetrate
Dry with radix scutellariae compatibility is must measuring for raw material, and when Rhizoma Belamcandae and radix scutellariae are with 1:1 compatibility, extract must measure maximum.
5 Rhizoma Belamcandae of test example, radix scutellariae, Rhizoma Belamcandae chromocor extract, skullcapflavone extract, Rhizoma Belamcandae skullcapflavone extract
The assay of belamcandin, scutelloside in object
Belamcandin assay in Rhizoma Belamcandae, Rhizoma Belamcandae chromocor extract, referring to 2015 editions P41~42 of Chinese Pharmacopoeia
It is carried out under " Rhizoma Belamcandae " item.
The assay of scutelloside in radix scutellariae, skullcapflavone extract, referring to 2015 editions P301~302 of Chinese Pharmacopoeia
It is carried out under " radix scutellariae " item.
Belamcandin, content determination of Baicalin in Rhizoma Belamcandae skullcapflavone extract
Belamcandin is measured according to high performance liquid chromatography (Chinese Pharmacopoeia four general rules 0512 in 2015)
Chromatographic condition and system suitability are using octadecylsilane chemically bonded silica as filler；With methanol-
0.02mol/L sodium dihydrogen phosphate (with phosphoric acid tune pH value to 3.0) (35:65) is mobile phase；Detection wavelength is 266nm.Reason
2500 should be not less than by calculating by plate number by belamcandin peak.
It is appropriate that the preparation precision of tectoridin reference substance solution weighs tectoridin reference substance, and 70% methanol aqueous solution is added and prepares
At the solution of every 1mL 0.05mg containing belamcandin to get.
The preparation of test solution takes Rhizoma Belamcandae skullcapflavone extract about 12.5mg in 25ml volumetric flask, is added 70%
Appropriate methanol aqueous solution, ultrasound to being completely dissolved, let cool, 70% methanol aqueous solution constant volume, shake up to get.
Measuring method draws reference substance solution and each 5 μ L of test solution respectively, injects liquid chromatograph, measures to obtain the final product.
This product (Rhizoma Belamcandae skullcapflavone extract, Rhizoma Belamcandae: radix scutellariae 1:1, W/W) is calculated by dry product contains belamcandin
(C22H24O11) 10% must not be less than.
Scutelloside is measured according to high performance liquid chromatography (Chinese Pharmacopoeia four general rules 0512 in 2015)
Chromatographic condition and system suitability are using octadecylsilane chemically bonded silica as filler；With methanol-water-phosphoric acid
(47:53:0.2) is mobile phase；Detection wavelength is 280nm.Number of theoretical plate is calculated by scutelloside peak should be not less than 2500.
It is appropriate that the preparation precision of scutelloside reference substance solution weighs scutelloside reference substance, and 70% methanol aqueous solution is added and prepares
At the solution of every 1mL 0.10mg containing scutelloside to get.
The preparation of test solution takes Rhizoma Belamcandae skullcapflavone extract about 12.5mg in 25ml volumetric flask, is added 70%
Appropriate methanol aqueous solution, ultrasound to being completely dissolved, let cool, 70% methanol aqueous solution constant volume, shake up to get.
Measuring method draws reference substance solution and each 5 μ L of test solution respectively, injects liquid chromatograph, measures to obtain the final product.
This product (Rhizoma Belamcandae skullcapflavone extract, Rhizoma Belamcandae: radix scutellariae 1:1, W/W) is calculated by dry product contains scutelloside
(C21H18O11) 22% must not be less than.
Assay the results are shown in Table 1.
Belamcandin, content determination of Baicalin result in 1 sample of table
* object amount is extracted: in terms of bulk pharmaceutical chemicals inventory 1kg；Belamcandin or scutelloside amount: object amount × belamcandin (radix scutellariae is extracted
Glycosides) content.
From upper table: when Rhizoma Belamcandae and radix scutellariae compatibility, the chromocor extract ingredient type extracted is more, when river is penetrated
When dry and radix scutellariae is with 1:1 compatibility, each flavones ingredient is more evenly.
6 radix scutellariae of test example is to Rhizoma Belamcandae solubilization
Method 1. must be measured from Rhizoma Belamcandae skullcapflavone extract and belamcandin, the scutelloside rate of transform investigate radix scutellariae to Rhizoma Belamcandae
Solubilized dissolution assistant effect
By 5 measurement result of test example, the rate of transform of belamcandin, scutelloside in sample is calculated, the results are shown in Table 2.
Belamcandin, scutelloside turn interest rate in 2 sample of table
| Sample | Extract must be measured | Belamcandin rate of transform * | Scutelloside rate of transform * |
| Rhizoma Belamcandae chromocor extract | 175.3 | 87.27 | |
| Skullcapflavone extract | 185.6 | 95.35 | |
| Rhizoma Belamcandae skullcapflavone extract is 1. | 207.8 | 93.55 | 94.92 |
| Rhizoma Belamcandae skullcapflavone extract is 2. | 208.5 | 93.59 | 95.24 |
| Rhizoma Belamcandae skullcapflavone extract is 3. | 212.3 | 93.72 | 95.83 |
| Rhizoma Belamcandae skullcapflavone extract is 4. | 203.7 | 90.49 | 95.29 |
| Rhizoma Belamcandae skullcapflavone extract is 5. | 200.2 | 88.93 | 94.63 |
* belamcandin or the scutelloside rate of transform, by taking Rhizoma Belamcandae the Baical Skullcap root P.E 2. middle belamcandin rate of transform as an example, the rate of transform
It calculates as follows:
As can be seen from the above table, Rhizoma Belamcandae skullcapflavone extract 1.~5. must measure and be all larger than same amount of Rhizoma Belamcandae or Huang
A kind of reed mentioned in ancient books chromocor extract must be measured, it was demonstrated that there are hydrotropies to be total to molten phenomenon for Rhizoma Belamcandae, radix scutellariae；From the scutelloside rate of transform, Rhizoma Belamcandae is yellow
There are few variations for the scutelloside rate of transform in a kind of reed mentioned in ancient books chromocor extract and skullcapflavone extract, it was demonstrated that Rhizoma Belamcandae is to radix scutellariae without solubilized hydrotropy
Effect；From the belamcandin rate of transform, the belamcandin rate of transform is greater than Rhizoma Belamcandae extracting flavonoids in Rhizoma Belamcandae skullcapflavone extract
The belamcandin rate of transform in object, it was demonstrated that radix scutellariae has solubilized dissolution assistant effect to Rhizoma Belamcandae；Comprehensive extract must measure and the belamcandin rate of transform,
When the ratio between Rhizoma Belamcandae and radix scutellariae medicinal materials are 1:1, as a result most preferably.
2. method takes Rhizoma Belamcandae chromocor extract, skullcapflavone extract, Rhizoma Belamcandae skullcapflavone extract 1.~5. and
Physical mixture is made in 1:9,3:7,5:5,7:3,9:1 ratio respectively in Rhizoma Belamcandae chromocor extract, skullcapflavone extract, altogether
12 samples, each sample weigh 1.0g, respectively plus 50ml boiling water and heat slightly boiled 1 minute, let cool to room temperature, be transferred to 50ml
In volumetric flask, water is added to be settled to scale, shaken up, stood overnight.Observation dissolution situation, solution shake and the G4 of weighed weight are used to hang down
Molten glass sand hourglass, filtering, 60 DEG C are dried under reduced pressure to constant weight, and weighing calculates the weight of insoluble matter, test result is shown in Table 3.
3 radix scutellariae of table is to Rhizoma Belamcandae solubilising dissolution assistant effect
As can be seen from the above table, radix scutellariae has solubilized dissolution assistant effect, Rhizoma Belamcandae skullcapflavone extract solubility to Rhizoma Belamcandae
Greater than its corresponding Rhizoma Belamcandae, the physical mixture of Baical Skullcap root P.E.
The identification of 7 Rhizoma Belamcandae skullcapflavone extract (Rhizoma Belamcandae: radix scutellariae 1:1) of test example
(1) this product about 25mg is taken, is placed in 50ml volumetric flask, scale is dissolved and be diluted to 70% ethyl alcohol, is shaken up, as
A liquid (0.5mg/ml).Precision draws A liquid 1ml in 50ml volumetric flask, dilute with 70% ethyl alcohol and is settled to scale and shakes up, work
For B liquid.B liquid shines UV-VIS spectrophotometry (2015 editions four general rules 0401 of Chinese Pharmacopoeia), using 70% ethyl alcohol as blank,
It is scanned in 200~400nm wave-length coverage, recording light spectrogram should have absorption maximum at 272 ± 2nm wavelength.
(2) it takes this product appropriate, adds methanol that solution of every 1ml containing 1mg is made, as test solution.Separately take Rhizoma Belamcandae pair
According to medicinal material, each 0.1g of radix scutellariae control medicinal material, add methanol 10ml, is ultrasonically treated 30 minutes, filtration, as reference substance solution.Separately take
Belamcandin, scutelloside reference substance are appropriate, add methanol that solution of every 1ml containing 0.2mg is made, as reference substance solution.According to thin layer color
Spectrometry (2015 editions four general rules 0401 of Chinese Pharmacopoeia) test, draws above-mentioned each 5 μ l of five kinds of solution, is put respectively in same silica gel
GF254On lamellae, with chloroform-methyl alcohol-formic acid (10:2:0.1) for solvent, it is unfolded, takes out, dry, set ultraviolet lamp
It is inspected under (254nm).In sample chromatogram, on position corresponding with reference medicine chromatography and reference substance chromatography, identical face is shown
The spot of color.
(3) it takes this product appropriate, adds methanol that solution of every 1ml containing 1mg is made, as test solution.Separately take Rhizoma Belamcandae pair
According to medicinal material, each 0.1g of radix scutellariae control medicinal material, add methanol 10ml, is ultrasonically treated 30 minutes, filtration, as reference substance solution.Separately take
Blackberry lily aglycon, baicalein reference substance are appropriate, add methanol that solution of every 1ml containing 0.2mg is made, as reference substance solution.According to thin layer
Chromatography (2015 editions four general rules 0401 of Chinese Pharmacopoeia) test, draws above-mentioned each 5 μ l of five kinds of solution, is put respectively in same silica gel
GF254On lamellae, with toluene-ethyl acetate-methyl alcohol-formic acid (10:3:1:0.5) for solvent, it is unfolded, takes out, dry, set
It is inspected under ultraviolet lamp (254nm).In sample chromatogram, on position corresponding with reference medicine chromatography and reference substance chromatography,
The spot of aobvious same color.
Above-mentioned ultraviolet spectrogram and thin-layer chromatogram are shown in Fig. 1~3.
Test example 8 Rhizoma Belamcandae skullcapflavone extract (Rhizoma Belamcandae: radix scutellariae 1:1) characteristic spectrum
It is measured according to high performance liquid chromatography (four general rules 0512 of Chinese Pharmacopoeia version in 2015).
Chromatographic condition and system suitability are using octadecylsilane bonded silica as filler；Using methanol as mobile phase A,
With 0.02mol/L sodium dihydrogen phosphate (with phosphoric acid tune pH value to 3.0) for Mobile phase B, the regulation according to the form below carries out gradient
Elution；Detection wavelength is 272nm.Number of theoretical plate is calculated by belamcandin peak should be not less than 2500.
| Time (minute) | Mobile phase A (%) | Mobile phase B (%) |
| 0~35 | 35 | 65 |
| 35~45 | 48 | 52 |
| 45~47 | 70 | 30 |
The preparation of reference solution takes tectoridin reference substance appropriate, accurately weighed, adds 70% methanol that every 1ml is made containing 50 μ g
Solution to get.
The preparation of test solution takes this product 12.5mg, accurately weighed, sets in 25ml measuring bottle, adds 70% methanol appropriate, surpasses
Sonication (power 400W, frequency 58KHz) 10 minutes, lets cool, is settled to scale, shake up to get.
Measuring method difference is accurate to draw reference solution and each 5 μ l of test solution, injects liquid chromatograph, measures, i.e.,
?.
There should be 19 characteristic peaks in test sample characteristic spectrum (Fig. 4), peak corresponding with object of reference peak is the peak S, calculates each spy
The relative retention time at peak and the peak S is levied, relative retention time should be within ± the 5% of specified value.Specified value are as follows: 0.939 (peak
1), 1.000 [peak 2 (S)], 1.063 (peaks 3), 1.147 (peaks 4), 1.237 (peaks 5), 1.275 (peaks 6), 1.476 (peaks 7), 1.722
(peak 8), 1.789 (peaks 9), 1.834 (peaks 10), 1.884 (peaks 11), 1.978 (peaks 12), 2.172 (peaks 13), 2.233 (peaks 14),
2.341 (peaks 15), 2.505 (peaks 16), 2.663 (peaks 17), 2.737 (peaks 18), 2.810 (peaks 19).
The influence of the drug paraxylene of the present invention of test example 9 cause mice auricle swelling
18~22g of weight mouse 150 is taken, half male and half female is randomly divided into 15 groups by gender, weight, and stomach-filling river is penetrated respectively
Dry chromocor extract, skullcapflavone extract, blackberry lily chromocor extract, blackberry lily skullcapflavone extract, Rhizoma Belamcandae skullcapflavone
Extract 1.~5., Rhizoma Belamcandae chromocor extract and skullcapflavone extract form in 1:9,3:7,5:5,7:3,9:1 ratio
Physical mixture and the isometric 1% tragacanth solution of blank control group ig.It above the equal successive administration of each group 6 days, gives within the 6th day
After medicine 30min, every mouse right ear coating 0.1ml causes scorching moulding, after 4 hours, mouse is pulled cervical vertebra and is put to death, is cut immediately
Mouse bilateral auricle lays ear disk at same position respectively with diameter 8mm punch, is weighed with electronic balance, right with every mouse
Auricle subtract left auricle weight be swelling index, by the swelling degree of control group and administration group be compared at statistics
Reason.As a result such as table 4.
The influence (X scholar SD) of the drug paraxylene of the present invention of table 4 cause mice auricle swelling
* blackberry lily chromocor extract dosage: identical weight medicinal material is converted by yield and is calculated, similarly hereinafter.
* blackberry lily skullcapflavone extract doses: identical weight medicinal material is converted by yield and is calculated, similarly hereinafter.
As can be seen from the above table, drug (Rhizoma Belamcandae skullcapflavone extract is 3.) paraxylene of the present invention causes Mice Auricle swollen
It is swollen to have significant inhibiting effect, and it is better than other each groups, that is, when the ratio between Rhizoma Belamcandae, radix scutellariae medicinal materials are 1:1, close and decoct upper tree
The resulting extractive of general flavone effect of rouge is best.
Influence of the drug of the present invention of test example 10 to mouse granuloma induced by implantation of cotton pellets
18~22g of weight mouse 150 is taken, half male and half female is randomly divided into 15 groups by gender, weight, uses in mouse chest
Iodine disinfection cuts an osculum in chest after 70% cotton ball soaked in alcohol takes off iodine, with ophthalmic tweezers by 5mg high pressure sterilization cotton balls from incision
It is implanted into subcutaneous axillary region, immediately skin suture.It was administered since the same day of performing the operation, 12 administration groups press 0.5g/kg dosage ig respectively
Administration, the isometric distilled water of control group ig, once a day.Above the equal successive administration of each group 7 days, after the 8th day administration 1h, by mouse
Disconnected neck is put to death, and implantation cotton balls is taken out together together with surrounding connective tissue, adipose tissue is rejected, is put into 60 DEG C of dryings 12 in baking oven
Hour, it weighs on an electronic balance.Cotton balls original weight amount is subtracted to get granuloma weight, is calculated with mg/10gbw, and carries out group
Between compare and statistical procedures.As a result such as table 5.
Influence (X scholar SD) of the drug of the present invention of table 5 to mouse granuloma induced by implantation of cotton pellets
As can be seen from the above table, drug (Rhizoma Belamcandae skullcapflavone extract is 3.) of the present invention has mouse granuloma induced by implantation of cotton pellets aobvious
The inhibiting effect of work, and it is better than other each groups, that is, when the ratio between Rhizoma Belamcandae, radix scutellariae medicinal materials are 1:1, close and decoct obtained by upper resin
Extractive of general flavone effect it is best.
The analgesic activity of the drug of the present invention of test example 11
18~22g of weight mouse 150 is taken, half male and half female is randomly divided into 15 groups by gender, weight, and ig administration is (right respectively
According to group to the distilled water of same volume), only, writhing is secondary in observation 15 minutes by every mouse ip 0.5%HAC0.2m1/ after sixty minutes for administration
Number.Result is compared statistics.It the results are shown in Table 6.
Analgesic activity (writhing method) (X soil SD) of the drug of the present invention of table 6 to mouse
As can be seen from the above table, drug (Rhizoma Belamcandae skullcapflavone extract is 3.) of the present invention has significant analgesic activity, and
It is better than other each groups, that is, when the ratio between Rhizoma Belamcandae, radix scutellariae medicinal materials are 1:1, close and decoct the upper resulting extractive of general flavone of resin
Effect is best.
The antitussive action of the drug of the present invention of test example 12
18~22g of weight mouse 150 is taken, half male and half female is randomly divided into 15 groups by gender, weight, and ig is administered 3 days respectively
(distilled water of the control group to same volume), last dose after forty minutes, mouse is placed in inverted 500ml beaker respectively,
0.15ml/ concentrated ammonia liquor is injected in the cuvette placed into beaker, through ammonia fumigating 15 seconds, is taken out mouse, is counted mouse 2 minutes
Cough number in time, and result is subjected to statistical disposition.It the results are shown in Table 7.
Antitussive action (X soil SD) of the drug of the present invention of table 7 to mouse
As can be seen from the above table, drug (Rhizoma Belamcandae skullcapflavone extract is 3.) of the present invention has significant antitussive action, and
It is better than other each groups, that is, when the ratio between Rhizoma Belamcandae, radix scutellariae medicinal materials are 1:1, close and decoct the upper resulting extractive of general flavone of resin
Effect is best.
To sum up, when Rhizoma Belamcandae and radix scutellariae compatibility, decocting liquid passes through D101The extract that purification with macroreticular resin technique obtains
It must measure, be significantly higher than same amount and individually using Rhizoma Belamcandae, blackberry lily, radix scutellariae or blackberry lily and radix scutellariae compatibility must measure as raw material, when river is penetrated
When dry and radix scutellariae is with 1:1 compatibility, extract must measure maximum.Tests prove that Rhizoma Belamcandae skullcapflavone extract of the present invention is anti-
Scorching, analgesia, antitussive action are substantially better than individual Rhizoma Belamcandae chromocor extract, skullcapflavone extract and Rhizoma Belamcandae, radix scutellariae Huang
The physical mixture of the various ratios of ketone extract is also better than the blackberry lily chromocor extract of same crude drug amount, blackberry lily skullcapflavone mentions
Object is taken, there is practical application value.
Claims (10)
1. a kind of Rhizoma Belamcandae skullcapflavone extract, it is characterised in that: it is extracted with water using Rhizoma Belamcandae and radix scutellariae as raw material,
Extracting solution is isolated and purified, filtering, dry manufactured extract.
2. extract according to claim 1, it is characterised in that: the mass ratio of the Rhizoma Belamcandae and radix scutellariae be 1:(0.1~
9)；It is preferred that Rhizoma Belamcandae and radix scutellariae mass ratio are 1:1.
3. the preparation method of extract as claimed in claim 1 or 2, it is characterised in that: it includes the following steps:
Medicinal material is weighed according to the ratio, grinds as smalls, is extracted with water, is filtered, and extracting solution is splined on large pore resin absorption column, successively uses water
It is eluted with alcohol, collects alcohol eluen, filtering, filtrate decompression is concentrated to give medicinal extract, and medicinal extract is dried under reduced pressure to get Rhizoma Belamcandae skullcapflavone
Extract.
4. preparation method according to claim 3, it is characterised in that: described be extracted with water is with 10~30 times of v/w medicinal material amounts
Water boiling and extraction 2~3 times, every time 1~2h, preferably with water boiling and extraction 3 times of 20 times of v/w medicinal material amounts, each 1h；The elder generation
It is afterwards first with the water elution of 1~3 times of v/w amount of resin, then 60~100% second with 5~7 times of v/w amount of resin with water and alcohol elution
Alcohol elution, preferably with the water elution of 2 times of v/w amount of resin, then 70% ethanol elution with 6 times of v/w amount of resin.
5. preparation method according to claim 3, it is characterised in that: the macroporous absorbent resin is D101Macroporous absorbent resin,
D101The dosage of macroporous absorbent resin is 3 times of medicinal material；And/or the temperature being dried under reduced pressure is 60 DEG C.
6. preparation method according to claim 5, it is characterised in that: the D101Macroporous absorbent resin is needed with 5%NaOH second
Alcoholic solution impregnates 8~12h, is allowed to dry, with water displacement wash to efflux pH value 7, is allowed to dry, then impregnates 8~12h with 5% hydrochloric acid solution,
It is allowed to dry, with water displacement wash to efflux pH value 7.
7. containing the preparation of extract as claimed in claim 1 or 2, which is characterized in that it is with Rhizoma Belamcandae skullcapflavone extract
For effective component, in addition the preparation that pharmaceutically acceptable auxiliary material is prepared.
8. preparation according to claim 7, which is characterized in that the preparation is oral preparation or injection.
9. preparation according to claim 8, which is characterized in that the oral preparation be paste, pill, oral solution, powder,
Tablet, granule or capsule；The injection is solution, freeze drying powder injection, tablet.
10. purposes of the extract as claimed in claim 1 or 2 in the drug for preparing anti-inflammatory, analgesia and/or cough-relieving.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910186239.5A CN109700899B (en) | 2019-03-12 | 2019-03-12 | Sichuan blackberry lily scutellaria baicalensis flavone extract and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910186239.5A CN109700899B (en) | 2019-03-12 | 2019-03-12 | Sichuan blackberry lily scutellaria baicalensis flavone extract and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109700899A true CN109700899A (en) | 2019-05-03 |
| CN109700899B CN109700899B (en) | 2021-10-08 |
Family
ID=66265782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910186239.5A Active CN109700899B (en) | 2019-03-12 | 2019-03-12 | Sichuan blackberry lily scutellaria baicalensis flavone extract and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109700899B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114965841A (en) * | 2021-02-20 | 2022-08-30 | 四川新绿色药业科技发展有限公司 | Method for identifying Sichuan blackberry lily and blackberry lily formula granules |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687099A (en) * | 2005-03-24 | 2005-10-26 | 深圳海王药业有限公司 | Extractive of general flavone from blackberry lily, preparation method and application in preparing medication |
| CN101185707A (en) * | 2006-11-23 | 2008-05-28 | 河北智同医药控股集团有限公司 | Antiphlogistic antiviral medicinal composition, and its preparation method and application |
| CN101987146A (en) * | 2010-09-17 | 2011-03-23 | 四川逢春制药有限公司 | Preparation method of capsule for treating pharyngitis and infection of upper respiratory tract and detection method of capsule |
| CN106963853A (en) * | 2017-04-11 | 2017-07-21 | 四川省中医药科学院 | A kind of Rhizoma Belamcandae total-flavonoid aglycone extract and its production and use |
| CN106995425A (en) * | 2017-04-20 | 2017-08-01 | 四川省中医药科学院 | The extraction process and its isolation and purification method of a kind of Rhizoma Belamcandae flavone aglycone |
-
2019
- 2019-03-12 CN CN201910186239.5A patent/CN109700899B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687099A (en) * | 2005-03-24 | 2005-10-26 | 深圳海王药业有限公司 | Extractive of general flavone from blackberry lily, preparation method and application in preparing medication |
| CN101185707A (en) * | 2006-11-23 | 2008-05-28 | 河北智同医药控股集团有限公司 | Antiphlogistic antiviral medicinal composition, and its preparation method and application |
| CN101987146A (en) * | 2010-09-17 | 2011-03-23 | 四川逢春制药有限公司 | Preparation method of capsule for treating pharyngitis and infection of upper respiratory tract and detection method of capsule |
| CN106963853A (en) * | 2017-04-11 | 2017-07-21 | 四川省中医药科学院 | A kind of Rhizoma Belamcandae total-flavonoid aglycone extract and its production and use |
| CN106995425A (en) * | 2017-04-20 | 2017-08-01 | 四川省中医药科学院 | The extraction process and its isolation and purification method of a kind of Rhizoma Belamcandae flavone aglycone |
Non-Patent Citations (3)
| Title |
|---|
| 王晓月,等: "黄芩射干汤中千层纸素A和次野鸢尾黄素的含量测定", 《实用药物与临床》 * |
| 王晓月,等: "黄芩射干汤中黄酮类成分指纹图谱的相关性", 《沈阳药科大学学报》 * |
| 王晓月,等: "黄芩射干汤移行成分经时变化规律初步研究", 《辽宁中医杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114965841A (en) * | 2021-02-20 | 2022-08-30 | 四川新绿色药业科技发展有限公司 | Method for identifying Sichuan blackberry lily and blackberry lily formula granules |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109700899B (en) | 2021-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110455965B (en) | Preparation method of pharmaceutical composition and HPLC fingerprint spectrum establishment method thereof | |
| CN111789918B (en) | Anti-coronavirus traditional Chinese medicine composition and preparation method and application thereof | |
| CN102579734B (en) | Traditional Chinese medicine composition of bone healing medicine, preparing method thereof and detecting method thereof | |
| CN101843667B (en) | Shuanghuanglian medicinal composition and preparation method thereof | |
| CN101829216B (en) | Preparation method and quality control method of traditional Chinese medicine preparation for treating bronchitis and bronchial asthma | |
| CN106620610A (en) | Preparation method of liquorice heart fire purging granule | |
| CN103816300B (en) | A kind of medicinal herb spray for treating skin disorder or mucous membrane cavity illness | |
| CN101269182B (en) | Pharmaceutical composition for treating children's wind-heat cold | |
| CN109700899A (en) | A kind of Rhizoma Belamcandae skullcapflavone extract and its preparation method and application | |
| CN102920964B (en) | Traditional Chinese medicine preparation for curing cough | |
| CN101940642A (en) | Chinese medicinal composition and application thereof | |
| CN101057895B (en) | 'Fuyanshu' preparation for treating gynopathy and its quality controlling method | |
| CN106309554B (en) | It is a kind of for preventing and/or treating the Baical Skullcap root P.E and its pharmaceutical composition of canker sore | |
| CN101108228A (en) | Pharmaceutical composition and method of preparing the same | |
| CN108635391A (en) | A kind of hempleaf groundsel herb phenolic acid components and the preparation method and application thereof | |
| CN113069516B (en) | Traditional Chinese medicine compound composition for preventing and treating cutaneous pruritus and application thereof | |
| CN101332216B (en) | Xingsiang Tuerfeng total phenolic acid extract and preparation method thereof | |
| WO2022036780A1 (en) | Test method for dampness-resolving and toxin-vanquishing composition | |
| CN100515476C (en) | Compound capsule with pseudo-ginseng and Chinese fanpalm seed, its preparation process and quality control method | |
| CN109187820B (en) | Method for establishing UPLC fingerprint of compound formula chinaroot greenbrier granules | |
| Tripathi et al. | Pharmacognostical standardization of nutmeg seeds (Myristica fragrans Houtt.)–a traditional medicine | |
| CN1961943A (en) | Pharmaceutical composition for treating cold, its preparation method and quality control method thereof | |
| Chaudhary et al. | Physico-Chemical Analysis and Preliminary Phytochemical Screening of OroxylumIndicum Root | |
| CN112578029A (en) | Lung-clearing decoction preparation HPLC quality control construction method and application thereof | |
| Aiyarakanchanakun et al. | Pharmacognostic specifications, RP-HPLC analysis of chlorogenic acid content and antioxidant activity of morus alba linn. leaves in Thailand |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |