CN109679356A - It is a kind of using alpha-acid in hop as bacteriostatic agent-preparation method of gelatine-chitosan edibility bacteriostatic film - Google Patents
It is a kind of using alpha-acid in hop as bacteriostatic agent-preparation method of gelatine-chitosan edibility bacteriostatic film Download PDFInfo
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- CN109679356A CN109679356A CN201910031292.8A CN201910031292A CN109679356A CN 109679356 A CN109679356 A CN 109679356A CN 201910031292 A CN201910031292 A CN 201910031292A CN 109679356 A CN109679356 A CN 109679356A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/463—Edible packaging materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/466—Bio- or photodegradable packaging materials
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/05—Alcohols; Metal alcoholates
- C08K5/053—Polyhydroxylic alcohols
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Abstract
The present invention provides one kind using hops alpha-acid as bacteriostatic agent, gelatin and chitosan are composite base material, glycerol is plasticizer, the edibility hops alpha-acid-gelatine-chitosan bacteriostatic film preparation method prepared using pure natural raw material, this method simple process, used raw material Nantural non-toxic, technique are environmentally protective.Prepared hops alpha-acid-gelatine-chitosan bacteriostatic film film liquid is the fruit direct impregnation film after can be directly used for picking to extend the shelf life, and can also be prepared into after film for the various food packagings such as various water fruits and vegetables, fresh food and bread, cake.
Description
Technical field
The present invention relates to a kind of preparation methods of hops alpha-acid-gelatine-chitosan bacteriostatic film, belong to new type functional packet
Package material field.More specifically, the present invention provides one kind using hops alpha-acid as bacteriostatic agent, gelatin and chitosan are composite base
Material, glycerol are plasticizer, the edibility hops alpha-acid-gelatine-chitosan bacteriostatic film preparation prepared using pure natural raw material
Method and its application.
Background technique
The deterioration of environment is the most stern challenge that human survival faces, and environmental protection has become global matter of utmost importance
One of.While the development of food packing industry is that economic construction is made that tremendous contribution, serious environment also is brought to the mankind
Pollution.This pollution is only second to air pollution, water pollution and ocean lake pollution, is as brought by food package film etc.
" white pollution ".Therefore, packaging is just put in face of the mankind with environmental protection as a hot spot of society, it has also become is badly in need of solution in the whole world
Serious problems certainly.
Edible film can replace Partial Food packaging, and pollution on the environment is effectively reduced, is with development prospect
Environmentally friendly packing material.Edible film is to be aided with edibility with the large biological molecule substance of edibility for main film forming matrix
Plasticizer, making to form a film by a series for the treatment of process interacts between agent molecule, and being allowed to form one kind after the drying has
The film of the compact structure of mechanical property and selective penetrated property.Edible film has the effect that (1) edible film is easily biological
Degradation, without any environmental pollution；(2) edible film can delay the migration of moisture and oil and other ingredients in food, diffusion；
(3) edible film is to CO2、O2Deng with selectable gas permeability；(4) food surface mechanical strength, which can be improved, in edible film makes it
Processing easy to process；(5) edible film can be used as food additives, such as pigment, antioxidant, preservative, flavour of food products material, sweet tea
The carrier of taste substance, nutrition fortifier etc.；(6) edible film can prevent flavor substance in food from volatilizing, and Effective Regulation food is storing up
Period and external environment and the transmitting between its interior tissue are deposited, to be greatly improved food quality, extends the shelf longevity
Life saves packaging material.
Hops is one of primary raw material of brewing, and the primary efficacy in beer is bacteriostasis, and beer
The main source of wine bitter taste and fragrance.In hops content highest be also main ingredient be hops in alpha-acid, have very
Strong bacteriostasis and a variety of physiological activity.China is the important hops growing area in the world, but the purposes of hops is relatively
It is single, substantially based on brewing.In order to expand the application field of hops, hops is utilized the present invention provides a kind of
In method of the alpha-acid as antibacterial additive preparation edibility bacteriostatic film, the bacteriostatic film of preparation can be used for numerous food system.
The natural bacteriostatic agent used the method overcome existing method is mostly mixed extract, and the mechanism of bacteriostasis is indefinite, addition
Relatively higher defect is measured, a kind of natural origin compound that bacteriostatic activity is strong is provided and cooks bacteriostatic agent, prepared film has
Better biocompatibility and bacteriostatic activity.This can not only expand the application market of hops, can also provide for food industry
A kind of alternative new packaging material can also be used in the fresh-keeping of fresh fruit, solve fresh fruit long-distance transport and storage
Time short problem.
Summary of the invention
Have the characteristics that degradable and highly-safe, edible food package and guarantor based on natural edible material
Fresh technology also has become one of the hot spot of current field of food research.The object of the present invention is to provide one kind with gelatin-chitosan
Sugar is substrate, glycerol is plasticizer, and hops alpha-acid is the preparation method of the edibility bacteriostatic film of bacteriostatic agent, this method suppression used
Microbial inoculum hops alpha-acid is the Important Economic crop in Xinjiang, and abundance, bacteriostatic activity is strong, is used in beer as bacteriostatic agent
The history of existing more than one thousand years.It is provided by the present invention that a kind of to prepare edibility hops alpha-acid-gelatine-chitosan edibility antibacterial
Film production process safety, in addition to using a small amount of ethyl alcohol and minimal amount of organic acid, whole process without using it is other it is toxic, have
Harmful organic solvent, and no waste discharge in production process, the coating liquid deployed can be entirely used for bacteriostatic film production, belong to
Environmentally protective technique.Due to hops alpha-acid have very strong physiological activity, chitosan itself also have certain biocidal property, two
Person, which combines, can significantly improve its bacteriostatic activity.Using this method preparation edibility bacteriostatic film have it is natural, nontoxic, harmless,
The features such as degradable.
To achieve the goals above, the present invention adopts the following technical scheme that be achieved:
A kind of preparation method of hops alpha-acid-gelatine-chitosan bacteriostatic film, it is characterised in that the following steps are included:
(1) by gelatin with 1:9 ~ 1:90(w/v) ratio be added in distilled water or deionized water, be placed in 45-75 DEG C of water-bath
Middle stirring is cooled to room temperature to dissolving, and the glycerol for being equivalent to 0.01 ~ 0.2 times of liquor capacity is added as plasticizer, continues in room
The lower stirring of temperature is clear and transparent to solution, stands, it is spare to obtain gelatin film liquid；
(2) ratio of chitosan 1:30 ~ 1:150(w/v) is added in the acetic acid aqueous solution that concentration is 0.2%-2% (v/v),
It dissolves by heating, is cooled to room temperature in 45 DEG C -85 DEG C of water-bath, addition is equivalent to 0.01 ~ 0.5 times of liquor capacity of glycerol conduct
Plasticizer continues that 1-2 h is stirred at room temperature, it is spare to obtain chitosan film liquid；
(3) the gelatin film liquid for respectively obtaining above-mentioned steps (1) and step (2) and chitosan film liquid are mixed by certain volume ratio
It closes, stirs 1-2 h, after coming into full contact with it uniformly, be added slowly with stirring the ethyl alcohol of preconfigured hops alpha-acid
Solution makes ultimate density 0.5 ~ 3 mg/mL of the hops alpha-acid in film forming solution, continues to stir 1-3 h at room temperature；Stop
It only stirs, solution stands 10-30 h, then 2 h of ultrasonic degassing at room temperature, the hops alpha-acid-gelatine-chitosan prepared
The film liquid of bacteriostatic film bacteriostatic film；
(4) the various fruits and vegetables for needing Preservation Treatment are totally immersed in above-mentioned steps (3) 2- in obtained bacteriostatic film film liquid
It 10 seconds, takes out, surface to be dried forms a film naturally, and vanning saves, and can be obviously prolonged the shelf life of fruit；
(5) film liquid of obtained bacteriostatic film in above-mentioned steps (3) is uniformly sprayed on to the various fruits and vegetables tables for needing Preservation Treatment
Face saves after vanning after drying surface and forming a film naturally, can be obviously prolonged the shelf life of fruit；
(6) the tape casting or spray coating method film will be passed through at film liquid obtained in above-mentioned steps (3), and in 25 DEG C -50 DEG C of condition
Under be dried, obtain the hops alpha-acid-gelatine-chitosan bacteriostatic film；The film is used for various food packagings, Neng Gouqi
It is acted on to good bacteriostasis, preservation, slows down its corrupt speed, hence it is evident that extend the shelf life of food.
A kind of hops alpha-acid-gelatine-chitosan bacteriostatic film, it is characterised in that each employed in bacteriostatic film preparation process
Kind solvent, bacteriostatic agent, filmogen gelatin and chitosan are the raw material of food-grade, prepared hops alpha-acid-gelatin-shell
Glycan bacteriostatic film is safe and non-toxic edible film.
A kind of hops alpha-acid-gelatine-chitosan bacteriostatic film, it is characterised in that preferred gelatin in the gelatin film liquid
It is 1:28(w/v), 1:22(w/v with the adding proportion of distilled water or deionized water) and 1:18(w/v).
A kind of hops alpha-acid-gelatine-chitosan bacteriostatic film, it is characterised in that preferred shell in the chitosan film liquid
The adding proportion of glycan and acetic acid aqueous solution is 1:88(w/v), 1:55(w/v) and 1:36(w/v).
A kind of hops alpha-acid-gelatine-chitosan bacteriostatic film, it is characterised in that the hops alpha-acid-gelatin-chitosan
Sugared bacteriostatic film is 1:2 ~ 2:1(v/v at the mixed volume ratio of gelatin film liquid in film liquid and chitosan film liquid).
A kind of hops alpha-acid-gelatine-chitosan bacteriostatic film, it is characterised in that the hops alpha-acid-gelatin-chitosan
Sugared bacteriostatic film is bacteriostatic agent at hops in film liquid, and the concentration range of the ethanol solution of preconfigured hops alpha-acid is 10
mg/mL~100 mg/mL。
A kind of hops alpha-acid-gelatine-chitosan bacteriostatic film at film liquid, be able to use the film liquid in various fruit-producing areas
Direct impregnation or various fruit in season surfaces are sprayed at when picking, refill case transport after surface forms a film naturally after film liquid, it can be bright
It is aobvious to delay fruit dehydration and rotten speed, so that its normal-temperature fresh-keeping time is extended 5 ~ 25 days, the preservation by low temperature time extends 10 ~ 35 days.
The film, can be used for the packaging of various fresh food, energy by a kind of hops alpha-acid-gelatine-chitosan bacteriostatic film
It is substantially reduced the dehydration and rotten speed of food, its normal-temperature fresh-keeping time is made to extend 5-25 days, the preservation by low temperature time extends 10 ~ 35
It.
A kind of hops alpha-acid-gelatine-chitosan bacteriostatic film of the present invention has bacteriostatic activity and antioxidant activity,
Tensile strength is greater than 10 MPa, and elongation at break is greater than 90%.
Pass through a kind of hops alpha-acid-gelatine-chitosan bacteriostatic film prepared by the above method, it is characterised in that the film is
A kind of bacteriostatic film of edibility can be used for various food, the packaging of health care product and various fruit freshness preservings as needed.
Beneficial effect obtained by the present invention is:
Extraction raw material selected by the present invention is natural origin, and is raw-food material or food additives, is belonged to edible
Raw material, it is from a wealth of sources.Meanwhile these substances also have biodegradable property, edible safety is reliable.Edibility is antibacterial
Film also has certain functional activity while providing packaging for food.It can reduce largely using plastic packing article and
Bring environmental problem, the convenience for improving the safety of food and using.
Simple production process of the present invention, production process are easy to control, to the of less demanding of equipment, in the process
Primary solvent is water, using only arrive a small amount of ethyl alcohol and acetic acid, it is easy to accomplish large-scale production.A kind of present invention beer obtained
Hop α acid-gelatine-chitosan bacteriostatic film have the advantages that it is safe and non-toxic, have good mechanical performance, barrier property, have both
The multiple efficacies such as antibacterial, anti-oxidant can according to need and be prepared into film for packaged food, health care product or other related productions
Product, also can directly film prolong storage period to the surface of food, facilitate storage and transport.
Specific embodiment
The present invention is explained in detail in by the way of specific embodiment below, but the present invention is not limited to the embodiment.
Embodiment 1
It takes 110 mL water in 500 mL beakers, 5g gelatin is added, stirring is cooled to room temperature to dissolving at 60 DEG C, and 0.9g is added
It is clear and transparent spare to solution to stir 1 h at 30 DEG C for glycerol；In 500 mL beakers, 2.2mL concentration is added is another 110 mL water
36% acetic acid mixes, adds 2g chitosan, is stirred at 70 DEG C to being completely dissolved, after being cooled to room temperature, mass concentration, which is added, is
2.2g glycerol stirs 1 h at 30 DEG C.The above-mentioned gelatin film liquid being prepared is mixed with chitosan film liquid, is stirred at 30 DEG C
After 1.5 h, continue to be added with stirring the ethanol solution 7mL that pre-configured mass concentration is 50mg/mL hops alpha-acid,
Make the concentration 1.5mg/mL of hops alpha-acid in film liquid, continue to stir 1 h at 30 DEG C, stand for 24 hours, then 2 h of ultrasonic degassing,
Hops alpha-acid-gelatine-chitosan bacteriostatic film the film liquid prepared.Film liquid is taped against on glass plate by the tape casting, 40
Dry 20 h are at DEG C up to hops alpha-acid-gelatine-chitosan bacteriostatic film.After measured, the tensile strength of the film is 12 MPa, is broken
Splitting elongation is 104%.
Embodiment 2
It takes 220 mL water in 500 mL beakers, 10g gelatin is added, stirring is cooled to room temperature to dissolving at 60 DEG C, is added
It is clear and transparent spare to solution to stir 1 h at 30 DEG C for 1.75g glycerol；It separately takes 220 mL water in 500 mL beakers, is added
4.5mL concentration is 36% acetic acid, mixes, adds 4g chitosan, stirred at 70 DEG C to being completely dissolved, after being cooled to room temperature, added
Entering mass concentration is 4.5g glycerol, stirs 1 h at 30 DEG C.The above-mentioned gelatin film liquid being prepared is mixed with chitosan film liquid,
After stirring 1.5 h at 30 DEG C, continuing to be added with stirring pre-configured mass concentration is 100mg/mL hops alpha-acid
Ethanol solution 7mL makes the concentration 1.5mg/mL of hops alpha-acid in film liquid, continues to stir 1 h at 30 DEG C, stand for 24 hours, then
2 h of ultrasonic degassing, the hops alpha-acid-gelatine-chitosan bacteriostatic film film liquid prepared.By the fresh deep above-mentioned film of immersion
In liquid, 5s is stood, is taken out, is dried in the air and put about 30 minutes or so, surface forms a film naturally, is put into room temperature preservation in crisper.With do not infiltrate
The comparison of strawberry sample, the freshness date of strawberry extends 5 days.
Embodiment 3
It takes 140 mL water in 500 mL beakers, 5g gelatin is added, stirring is cooled to room temperature to dissolving at 60 DEG C, and 1.2g is added
It is clear and transparent spare to solution to stir 1 h at 30 DEG C for glycerol；Separately take 140 mL water in 500 mL beakers, 2mL concentration, which is added, is
36% acetic acid mixes, adds 3.8g chitosan, is stirred at 70 DEG C to being completely dissolved, after being cooled to room temperature, mass concentration is added
For 2.5g glycerol, 1 h is stirred at 30 DEG C.The above-mentioned gelatin film liquid being prepared is mixed with chitosan film liquid, is stirred at 30 DEG C
After mixing 2 h, continue to be added with stirring the ethanol solution 6 that pre-configured mass concentration is 50mg/mL hops alpha-acid
ML makes the concentration 1.0mg/mL of hops alpha-acid in film liquid, continues to stir 1 h at 30 DEG C, stand for 24 hours, then ultrasonic degassing 2
H, the hops alpha-acid-gelatine-chitosan bacteriostatic film film liquid prepared.By the edibility bacteriostatic film grab sample of preparation
It is cut to the strip of 1 × 1 cm, is placed in test tube, 5 mL ethyl alcohol are added, discharge its bacteriostatic agent to balance.It sequentially adds
1.00 mL bacteriostatic agent release liquids, 3.00 mL, 40 μ g/mL DPPH solution, after being protected from light 30 min, make blank with ethyl alcohol,
Absorbance is measured at 517 nm.Calculating the edibility bacteriostatic film DPPH clearance rate is 46% ~ 55%.
Embodiment 4
It takes 100mL water in 500 mL beakers, 5.5 gelatin is added, stirring is cooled to room temperature to dissolving at 60 DEG C, and 0.8g is added
It is clear and transparent spare to solution to stir 2 h at 30 DEG C for glycerol；It separately takes 100 mL water in 500 mL beakers, 1.8mL concentration is added
It for 36% acetic acid, mixes, adds 1.8g chitosan, stirred at 70 DEG C to being completely dissolved, after being cooled to room temperature, it is dense that quality is added
Degree is 2g glycerol, stirs 1 h at 30 DEG C.The above-mentioned gelatin film liquid being prepared is mixed with chitosan film liquid, is stirred at 30 DEG C
After mixing 1.5 h, continue to be added with stirring the ethanol solution that pre-configured mass concentration is 100mg/mL hops alpha-acid
3mL makes the concentration 1.5mg/mL of hops alpha-acid in film liquid, continues to stir 2 h at 30 DEG C, stand for 24 hours, then ultrasonic degassing 2
H, the hops alpha-acid-gelatine-chitosan bacteriostatic film film liquid prepared.Take shigella sonnei (CMCC(B) 51592)
Nutrient broth is aseptically added in strain, spreads cultivation, and 24 h are cultivated at 37 DEG C.It chooses colonies typical and is dissolved in
In physiological saline 0.85%(w/v), make the 0.50 Maxwell turbidity unit of registration of bacterial turbidity instrument, concentration is 1.5 × 108
cfu/mL.Above-mentioned 0.1 mL of bacteria suspension is drawn in culture medium with sterile tube, is uniformly mixed while hot by mixed bacterium method and is poured into culture
In ware.Diaphragm is tightly attached to media surface with aseptic nipper afterwards, after 24 h are cultivated at 37 DEG C, measures antibacterial circle diameter size
For 16 mm.
Claims (8)
1. a kind of using α acid in hops as the antibacterial membrane preparation method of gelatine-chitosan edibility of bacteriostatic agent, it is characterised in that packet
Include following steps:
(1) by gelatin with 1:9 ~ 1:90(w/v) ratio be added in distilled water or deionized water, be placed in 45-75 DEG C of water-bath
Middle stirring is cooled to room temperature to dissolving, and the glycerol for being equivalent to 0.01 ~ 0.2 times of liquor capacity is added as plasticizer, continues in room
The lower stirring of temperature is clear and transparent to solution, stands, it is spare to obtain gelatin film liquid；
(2) ratio of chitosan 1:30 ~ 1:150(w/v) is added in the acetic acid aqueous solution that concentration is 0.2%-2% (v/v),
It dissolves by heating, is cooled to room temperature in 45 DEG C -85 DEG C of water-bath, addition is equivalent to 0.01 ~ 0.5 times of liquor capacity of glycerol conduct
Plasticizer continues that 1-2 h is stirred at room temperature, it is spare to obtain chitosan film liquid；
(3) the gelatin film liquid for respectively obtaining above-mentioned steps (1) and step (2) and chitosan film liquid are mixed by certain volume ratio
It closes, stirs 1-2 h, after coming into full contact with it uniformly, be added slowly with stirring the ethyl alcohol of preconfigured hops alpha-acid
Solution makes ultimate density 0.5 ~ 3 mg/mL of the hops alpha-acid in film forming solution, continues to stir 1-3 h at room temperature；Stop
It only stirs, solution stands 10-30 h, then 2 h of ultrasonic degassing at room temperature, the hops alpha-acid-gelatine-chitosan prepared
The film liquid of bacteriostatic film bacteriostatic film；
(4) the various fruits and vegetables for needing Preservation Treatment are totally immersed in above-mentioned steps (3) 2- in obtained bacteriostatic film film liquid
It 10 seconds, takes out, surface to be dried forms a film naturally, and vanning saves, and can be obviously prolonged the shelf life of fruit；
(5) film liquid of obtained bacteriostatic film in above-mentioned steps (3) is uniformly sprayed on to the various fruits and vegetables tables for needing Preservation Treatment
Face saves after vanning after drying surface and forming a film naturally, can be obviously prolonged the shelf life of fruit；
(6) the tape casting or spray coating method film will be passed through at film liquid obtained in above-mentioned steps (3), and in 25 DEG C -50 DEG C of condition
Under be dried, obtain the hops alpha-acid-gelatine-chitosan bacteriostatic film；The film is used for various food packagings, Neng Gouqi
It is acted on to good bacteriostasis, preservation, slows down its corrupt speed, hence it is evident that extend the shelf life of food.
2. according to the method described in claim 1, it is characterized in that various solvents employed in bacteriostatic film preparation process, antibacterial
Agent, filmogen gelatin and chitosan are the raw material of food-grade, prepared hops alpha-acid-gelatine-chitosan bacteriostatic film
For safe and non-toxic edible film.
3. method described in step (1) according to claim 1, it is characterised in that preferred gelatin and steaming in the gelatin film liquid
The adding proportion of distilled water or deionized water is 1:28(w/v), 1:22(w/v) and 1:18(w/v).
4. method described in step (2) according to claim 1, it is characterised in that preferred chitosan in the chitosan film liquid
Adding proportion with acetic acid aqueous solution is 1:88(w/v), 1:55(w/v) and 1:36(w/v).
5. method described in step (3) according to claim 1, it is characterised in that the hops alpha-acid-gelatine-chitosan suppression
Mycoderm is 1:2 ~ 2:1(v/v at the mixed volume ratio of gelatin film liquid in film liquid and chitosan film liquid).
6. method described in step (3) according to claim 1, it is characterised in that the hops alpha-acid-gelatine-chitosan suppression
Mycoderm is bacteriostatic agent at hops in film liquid, and the concentration range of the ethanol solution of preconfigured hops alpha-acid is 10 mg/
mL~100 mg/mL。
7. hops alpha-acid-gelatine-chitosan bacteriostatic film film forming described in step (4) and step (5) according to claim 1
Liquid, it is characterised in that be able to use the film liquid and direct impregnation or be sprayed at various fruit in season tables when various fruit-producing areas are picked
Face refills case transport after film liquid after surface forms a film naturally, can obviously delay fruit dehydration and rotten speed, make its normal-temperature fresh-keeping
Time extends 5 ~ 25 days, and the preservation by low temperature time extends 10 ~ 35 days.
8. hops alpha-acid-gelatine-chitosan bacteriostatic film described in step (6) according to claim 1, it is characterised in that can incite somebody to action
The film is used for the packaging of various fresh food, can be substantially reduced the dehydration and rotten speed of food, prolong its normal-temperature fresh-keeping time
5-25 days long, the preservation by low temperature time extends 10 ~ 35 days.
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| CN201910031292.8A CN109679356A (en) | 2019-01-14 | 2019-01-14 | It is a kind of using alpha-acid in hop as bacteriostatic agent-preparation method of gelatine-chitosan edibility bacteriostatic film |
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| CN201910031292.8A CN109679356A (en) | 2019-01-14 | 2019-01-14 | It is a kind of using alpha-acid in hop as bacteriostatic agent-preparation method of gelatine-chitosan edibility bacteriostatic film |
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| US20090155328A1 (en) * | 2007-12-14 | 2009-06-18 | E. I. Du Pont De Nemours And Company | Films comprising antimicrobial and fungistatic agents |
| CN103275358A (en) * | 2013-05-20 | 2013-09-04 | 吉林大学 | Method for preparing chitosan-based composite preservative film or coating |
| CN106750580A (en) * | 2017-01-09 | 2017-05-31 | 福州大学 | A kind of new edibility antibacterial food packaging film and preparation method thereof |
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2019
- 2019-01-14 CN CN201910031292.8A patent/CN109679356A/en active Pending
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| US20030015480A1 (en) * | 2000-03-08 | 2003-01-23 | Breen Alexander W. | Methods of using hop acids to control organisms |
| US20090155328A1 (en) * | 2007-12-14 | 2009-06-18 | E. I. Du Pont De Nemours And Company | Films comprising antimicrobial and fungistatic agents |
| CN103275358A (en) * | 2013-05-20 | 2013-09-04 | 吉林大学 | Method for preparing chitosan-based composite preservative film or coating |
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