CN109674055A - A kind of extraction process of broccoli sprout extract - Google Patents
A kind of extraction process of broccoli sprout extract Download PDFInfo
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- CN109674055A CN109674055A CN201910108244.4A CN201910108244A CN109674055A CN 109674055 A CN109674055 A CN 109674055A CN 201910108244 A CN201910108244 A CN 201910108244A CN 109674055 A CN109674055 A CN 109674055A
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- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 title claims abstract description 101
- 240000003259 Brassica oleracea var. botrytis Species 0.000 title claims abstract description 101
- 235000017647 Brassica oleracea var italica Nutrition 0.000 title claims abstract description 100
- 239000000284 extract Substances 0.000 title claims abstract description 54
- 238000000605 extraction Methods 0.000 title claims abstract description 42
- 239000000843 powder Substances 0.000 claims abstract description 34
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 24
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 238000012545 processing Methods 0.000 claims abstract description 8
- 239000000287 crude extract Substances 0.000 claims description 25
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 13
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 241000220259 Raphanus Species 0.000 claims description 8
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 8
- 238000013019 agitation Methods 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- 235000019441 ethanol Nutrition 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 7
- SRRKNRDXURUMPP-UHFFFAOYSA-N sodium disulfide Chemical compound [Na+].[Na+].[S-][S-] SRRKNRDXURUMPP-UHFFFAOYSA-N 0.000 claims description 7
- 238000000967 suction filtration Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000002024 ethyl acetate extract Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 230000008859 change Effects 0.000 abstract description 2
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 24
- QKGJFQMGPDVOQE-UHFFFAOYSA-N Sulforaphen Natural products CS(=O)C=CCCN=C=S QKGJFQMGPDVOQE-UHFFFAOYSA-N 0.000 description 10
- QKGJFQMGPDVOQE-HWKANZROSA-N raphanin Chemical compound CS(=O)\C=C\CCN=C=S QKGJFQMGPDVOQE-HWKANZROSA-N 0.000 description 10
- 238000011084 recovery Methods 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 235000015110 jellies Nutrition 0.000 description 5
- 239000008274 jelly Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 201000011510 cancer Diseases 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 239000012043 crude product Substances 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- -1 Victoria C Chemical compound 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003810 ethyl acetate extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000019508 mustard seed Nutrition 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 108010058651 thioglucosidase Proteins 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention is a kind of extraction process of broccoli sprout extract, and the extraction process of broccoli sprout extract includes the following steps；The selection of broccoli sprout, the processing of broccoli sprout, sprout powder extracts, prepared by broccoli sprout extract；Since the present invention optimizes broccoli sprout traditional extraction technique, change enzymolysis agent composition, the yield of broccoli sprout extract thus can be improved, possess relatively good purity in not purified situation, with preferable quality and stability, the application range of broccoli sprout extract, additional income can be expanded.
Description
Technical field
The invention belongs to technical field of plant extraction, and in particular to a kind of extraction process of broccoli sprout extract.
Background technique
Science and technology is stepped up in progress, people's living standard, and the life of health-nutrition becomes mainstream gradually.Contain in vegetables
A large amount of vitamin and microelement needed by human, are the indispensable parts of health of people diet.Moreover, further research
It it was found that there are also cancer-resisting substances in brassicaceous vegetable, can effectively inhibit tumour, cancer cell growth and formation, prevent disease
Occur.
Cancer-resisting substance sulforaphen rich in broccoli, can be used for the prevention and treatment of cancer, but mentions at present
It takes the cost of material of sulforaphen higher, is unfavorable for industrialization production, moreover, using a large amount of organic solvents in extraction process, hold
It easily pollutes the environment, extremely not environmentally.
Therefore, how Optimized Extraction Process, improve extraction efficiency, to reduce production cost, improve raw material and utilize, reduce ring
Border pollution, it appears particularly important.
Chinese patent CN103436565B discloses a kind of method that sulforaphen is extracted from broccoli, and this method includes
Following steps: it dries to constant weight and crushes after being (1) dehydrated the edible part spray of fresh broccoli, obtain broccoli dry powder；(2) will
Removal of impurities is filtered by vacuum after crushing in the mustard seed newly harvested, obtains crude enzyme liquid；(3) the crude enzyme liquid is added into the broccoli dry powder,
It stirs evenly, is extracted through enzymatic hydrolysis, supersonic oscillations, obtain extracting solution, extracting solution sieving slagging-off obtains filtrate；Filtrate is through true
Empty pump is filtered up to sulforaphen crude extract；(4) the thick water lift solution of sulforaphen will be obtained after sulforaphen crude extract vacuum concentration；
(5) add ethyl acetate extraction into the thick water lift solution of sulforaphen, obtain ethyl acetate phase, which is concentrated in vacuo to cream
Then shape elutes and is concentrated in vacuo to paste again to get paste sulforaphen crude extract；(6) paste sulforaphen crude extract is chilled
The dry sulforaphen crude product for being 1%~15% to get purity to constant weight.But the extraction efficiency of crude enzyme liquid is low in the invention
Under, yield cannot be improved.
Chinese patent CN106963800A discloses a kind of preparation method of Caulis et Folium Brassicae capitatae, includes the following steps, takes
Petroleum ether is added in broccoli dry powder, is uniformly mixed using high-speed homogenization machine, and filtering obtains filtrate after degreasing；In filtrate
The Tris-HCL buffer and myrosinase of 49-51mmol/L, pH4.6-4.9,26-30 DEG C of enzymatic hydrolysis 5-8h, enzyme is added
After the completion of solution, the ethyl acetate that 2-4 times of volume is added is extracted, and extraction is also concentrated in vacuo at 35-40 DEG C, to obtain
Obtain Caulis et Folium Brassicae capitatae crude product；By Caulis et Folium Brassicae capitatae crude product carry out silica gel column chromatography, using the mixed liquor of chloroform and methanol into
Row elution is collected Caulis et Folium Brassicae capitatae eluent, is concentrated in vacuo at 35-40 DEG C, to obtain Caulis et Folium Brassicae capitatae, the present invention also public affairs
The application of the extract and extract of a kind of above method preparation has been opened, above-mentioned Caulis et Folium Brassicae capitatae can be used for treating breast cancer,
But it is more using the quantity of organic solvent in the invention, recycling is difficult, to the seriously polluted of environment after discarding.
Summary of the invention
The object of the present invention is to provide a kind of extraction processes of broccoli sprout extract, solve current broccoli and extract
Higher cost in the process is unable to industrialization, while extraction process pollution is larger, not enough environmentally friendly problem.
To solve above-mentioned deficiency, it is found that the extraction process of better broccoli sprout extract, the present invention provide following
Technical solution:
A kind of extraction process of broccoli sprout extract, steps are as follows for extraction process:
S10, the selection of broccoli sprout: choosing the broccoli sprout sprouted 4 days~6 days, and clear water washing drains, spare；
S20, the processing of broccoli sprout: the broccoli sprout drained in S10 is placed in the baking oven that temperature is 65 DEG C~75 DEG C,
10s is irradiated in the UV lamp after pulverizing and sieving after baking 20min~35min, obtains sprout powder, it is spare；What is dried is blue and white
Dish sprout powder is good with the compatibility of extractant during the extraction process, and recovery rate can be improved, and using ultraviolet light irradiation, can remove
The miscellaneous purity and recovery rate for improving broccoli sprout extract；
S30, sprout powder extract: in S20 in sprout powder be added mixing extractant so that solid-liquid ratio be 1:1.6~
2.2, pH is adjusted after stirring 3min~6min, 35 DEG C~55 DEG C is then heated to and extracts, will be obtained after extracting solution centrifugation, suction filtration
To broccoli sprout crude extract；
S40, the preparation of broccoli sprout extract: broccoli sprout crude extract is extracted 2 times repeatedly using ethyl acetate in S30
Afterwards under the conditions of temperature is 40 DEG C~44 DEG C, the 1/6~1/ of broccoli sprout crude extract volume is evaporated to using Rotary Evaporators
3, it is subsequently placed in freeze dryer and is lyophilized, obtain the broccoli sprout extract.
Further, in S20, the mesh number of crushing is 100 mesh~200 mesh.
Further, in S30, mixing extractant be methanol, ethyl alcohol, Victoria C, sodium disulfide and deionized water in mass ratio
The mixed solution that 0.2:0.5:0.4:0.05:1~1.5 is formed.Mixing extractant can significantly improve extraction efficiency, improve and extract
The purity of object, simplification of flowsheet reduce cost and energy consumption.
Further, in S30, pH is adjusted to 4.85~5.05.Suitable pH can significantly improve extraction efficiency, shorter
It completes to extract in time, reduces cost.
Further, in S30, extract specifically: by mixed liquor power be 300W~350W, frequency be 65KHz~
Under the conditions of 85KHz, then enzymolysis agent is added in ultrasonic 35min~45min, under the conditions of temperature is 25 DEG C~30 DEG C, digests 4.5h
~6.5h.
Further, enzymolysis agent and the volume ratio of mixed liquor are 0 .25~0.36:1.5.
Further, enzymatic hydrolysis carries out magnetic agitation simultaneously with the rate of 160r/min~220r/min.Stirring can add
Speed enzymatic hydrolysis, provides a uniform and stable enzymatic hydrolysis environment for system.
Further, enzymolysis agent is the mixture that myrosin and radish dry powder 1:0.5 in mass ratio~0.6 are formed.
Myrosin and radish dry powder are applied in combination, and can guarantee enzymatic hydrolysis to avoid the enzymatic hydrolysis unicity and imperfection of single enzyme solution agent
Completely and improve enzymolysis efficiency and recovery rate.
Further, in S30, centrifugation is specifically, be centrifuged 25s under conditions of revolving speed is 3500r/min~4500r/min
~35s.
Further, in S40, freeze-drying under the conditions of temperature is -38 DEG C~-42 DEG C specifically, be lyophilized 4.0h~4.5h.
It can be seen from above-mentioned technical proposal the invention has the following advantages:
(1) since the present invention optimizes broccoli sprout traditional extraction technique, change enzymolysis agent composition, thus blueness can be improved
The yield of cauliflower sprout extract possesses relatively good purity in not purified situation, has preferable quality and stability,
The application range of broccoli sprout extract, additional income can be expanded；
(2) since the extraction process of broccoli sprout of the present invention is simple, with material abundance, thus broccoli sprout is mentioned
Taking technique strong operability can reduce the cost input of energy consumption and extraction process, it can be achieved that low cost, high yield；
(3) organic solvent as used in broccoli sprout extraction process of the present invention is less, thus can be to avoid blue and white
In dish sprout extraction process to environment generate pollution, influence ecological, environmental protective and personal safety, have it is green safe, ecological, environmental protective
Feature.
Below by specific embodiment, the present invention is described in further detail.
Specific embodiment
Present invention will be further explained by specific examples below, it should be understood, however, that, these embodiments are only
It is used, is but should not be understood as present invention is limited in any form for specifically describing in more detail.
The present invention carries out general and/or specific description to the material and test method arrived used in test.Though
So many materials and operating method used in purpose are it is known in the art that still the present invention is still herein to realize the present invention
It is described in detail as far as possible.It will be apparent to those skilled in the art that hereinafter, if not specified, material therefor of the present invention and
Operating method is well known in the art.
Embodiment 1
A kind of extraction process of broccoli sprout extract
Include the following steps:
S10, the selection of broccoli sprout: choosing the broccoli sprout sprouted 4 days, and clear water washing drains, spare；
S20, the processing of broccoli sprout: the broccoli sprout drained in S10 is placed in the baking oven that temperature is 65 DEG C, baking
It is crushed after 20min and irradiates 10s in the UV lamp after sieving with 100 mesh sieve, obtain sprout powder, it is spare；
S30, sprout powder extract: mixing extractant are added in S20 in sprout powder, so that solid-liquid ratio is 1:1.6, stirring
PH to 4.85 is adjusted after 3min, is then heated to 35 DEG C by mixed liquor under the conditions of power is 300W, frequency is 65KHz, is surpassed
Sound 35min, is then added enzymolysis agent, under the conditions of temperature is 25 DEG C, digests 4.5h, is finally 3500r/ in revolving speed by extracting solution
Broccoli sprout crude extract is obtained after being centrifuged 25s, suction filtration under conditions of min；
Wherein, mixing extractant is methanol, ethyl alcohol, Victoria C, sodium disulfide and deionized water 0.2:0.5:0.4 in mass ratio:
The mixed solution of 0.05:1 composition；Enzymolysis agent and the volume ratio of mixed liquor are 0 .25:1.5；Enzymatic hydrolysis is simultaneously with 160r/min's
Rate carries out magnetic agitation；Enzymolysis agent is the mixture that myrosin and radish dry powder 1:0.5 in mass ratio are formed.
S40, the preparation of broccoli sprout extract: broccoli sprout crude extract is extracted 2 times repeatedly using ethyl acetate in S30
Afterwards under the conditions of temperature is 40 DEG C, it is evaporated to the 1/6 of broccoli sprout crude extract volume using Rotary Evaporators, is subsequently placed in jelly
4.0h is lyophilized under the conditions of temperature is -38 DEG C in dry machine, obtains the broccoli sprout extract.Broccoli bud is obtained through detection
The recovery rate of seedling extract is 13.15%.
Embodiment 2
A kind of extraction process of broccoli sprout extract
Include the following steps:
S10, the selection of broccoli sprout: choosing the broccoli sprout sprouted 6 days, and clear water washing drains, spare；
S20, the processing of broccoli sprout: the broccoli sprout drained in S10 is placed in the baking oven that temperature is 75 DEG C, baking
10s is irradiated in the UV lamp after crushed 200 meshes after 35min, obtains sprout powder, it is spare；
S30, sprout powder extract: mixing extractant are added in S20 in sprout powder, so that solid-liquid ratio is 1:2.2, stirring
PH to 5.05 is adjusted after 6min, is then heated to 55 DEG C by mixed liquor under the conditions of power is 350W, frequency is 85KHz, is surpassed
Sound 45min, is then added enzymolysis agent, under the conditions of temperature is 30 DEG C, digests 6.5h, is finally 4500r/ in revolving speed by extracting solution
Broccoli sprout crude extract is obtained after being centrifuged 35s, suction filtration under conditions of min；
Wherein, mixing extractant is methanol, ethyl alcohol, Victoria C, sodium disulfide and deionized water 0.2:0.5:0.4 in mass ratio:
The mixed solution of 0.05:1.5 composition；Enzymolysis agent and the volume ratio of mixed liquor are 0.36:1.5；Enzymatic hydrolysis is simultaneously with 220r/min
Rate carry out magnetic agitation；Enzymolysis agent is the mixture that myrosin and radish dry powder 1:0.6 in mass ratio are formed.
S40, the preparation of broccoli sprout extract: broccoli sprout crude extract is extracted 2 times repeatedly using ethyl acetate in S30
Afterwards under the conditions of temperature is 44 DEG C, it is evaporated to the 1/3 of broccoli sprout crude extract volume using Rotary Evaporators, is subsequently placed in jelly
4.5h is lyophilized under the conditions of temperature is -42 DEG C in dry machine, obtains the broccoli sprout extract.Broccoli bud is obtained through detection
The recovery rate of seedling extract is 12.32%.
Embodiment 3
A kind of extraction process of broccoli sprout extract
Include the following steps:
S10, the selection of broccoli sprout: choosing the broccoli sprout sprouted 6 days, and clear water washing drains, spare；
S20, the processing of broccoli sprout: the broccoli sprout drained in S10 is placed in the baking oven that temperature is 67 DEG C, baking
10s is irradiated in the UV lamp after crushed 120 meshes after 25min, obtains sprout powder, it is spare；
S30, sprout powder extract: mixing extractant are added in S20 in sprout powder, so that solid-liquid ratio is 1:1.8, stirring
PH to 4.88 is adjusted after 4min, is then heated to 38 DEG C by mixed liquor under the conditions of power is 310W, frequency is 70KHz, is surpassed
Sound 37min, is then added enzymolysis agent, under the conditions of temperature is 26 DEG C, digests 5.0h, is finally 3800r/ in revolving speed by extracting solution
Broccoli sprout crude extract is obtained after being centrifuged 27s, suction filtration under conditions of min；
Wherein, mixing extractant is methanol, ethyl alcohol, Victoria C, sodium disulfide and deionized water 0.2:0.5:0.4 in mass ratio:
The mixed solution of 0.05:1.1 composition；Enzymolysis agent and the volume ratio of mixed liquor are 0 .29:1.5；Enzymatic hydrolysis is simultaneously with 180r/min
Rate carry out magnetic agitation；Enzymolysis agent is the mixture that myrosin and radish dry powder 1:0.6 in mass ratio are formed.
S40, the preparation of broccoli sprout extract: broccoli sprout crude extract is extracted 2 times repeatedly using ethyl acetate in S30
Afterwards under the conditions of temperature is 41 DEG C, it is evaporated to the 1/5 of broccoli sprout crude extract volume using Rotary Evaporators, is subsequently placed in jelly
4.5h is lyophilized under the conditions of temperature is -39 DEG C in dry machine, obtains the broccoli sprout extract.Broccoli bud is obtained through detection
The recovery rate of seedling extract is 13.08%.
Embodiment 4
A kind of extraction process of broccoli sprout extract
Include the following steps:
S10, the selection of broccoli sprout: choosing the broccoli sprout sprouted 4 days, and clear water washing drains, spare；
S20, the processing of broccoli sprout: the broccoli sprout drained in S10 is placed in the baking oven that temperature is 73 DEG C, baking
10s is irradiated in the UV lamp after crushed 180 meshes after 33min, obtains sprout powder, it is spare；
S30, sprout powder extract: mixing extractant are added in S20 in sprout powder, so that solid-liquid ratio is 1:2.1, stirring
PH to 5.02 is adjusted after 5min, is then heated to 50 DEG C by mixed liquor under the conditions of power is 340W, frequency is 80KHz, is surpassed
Sound 42min, is then added enzymolysis agent, under the conditions of temperature is 29 DEG C, digests 6.0h, is finally 4100r/ in revolving speed by extracting solution
Broccoli sprout crude extract is obtained after being centrifuged 33s, suction filtration under conditions of min；
Wherein, mixing extractant is methanol, ethyl alcohol, Victoria C, sodium disulfide and deionized water 0.2:0.5:0.4 in mass ratio:
The mixed solution of 0.05:1.4 composition；Enzymolysis agent and the volume ratio of mixed liquor are 0 .34:1.5；Enzymatic hydrolysis is simultaneously with 200r/min
Rate carry out magnetic agitation；Enzymolysis agent is the mixture that myrosin and radish dry powder 1:0.5 in mass ratio are formed.
S40, the preparation of broccoli sprout extract: broccoli sprout crude extract is extracted 2 times repeatedly using ethyl acetate in S30
Afterwards under the conditions of temperature is 43 DEG C, it is evaporated to the 1/4 of broccoli sprout crude extract volume using Rotary Evaporators, is subsequently placed in jelly
4.0h is lyophilized under the conditions of temperature is -41 DEG C in dry machine, obtains the broccoli sprout extract.Broccoli bud is obtained through detection
The recovery rate of seedling extract is 12.56%.
Embodiment 5
A kind of extraction process of broccoli sprout extract
Include the following steps:
S10, the selection of broccoli sprout: choosing the broccoli sprout sprouted 5 days, and clear water washing drains, spare；
S20, the processing of broccoli sprout: the broccoli sprout drained in S10 is placed in the baking oven that temperature is 69 DEG C, baking
10s is irradiated in the UV lamp after crushed 150 meshes after 28min, obtains sprout powder, it is spare；
S30, sprout powder extract: mixing extractant are added in S20 in sprout powder, so that solid-liquid ratio is 1:1.9, stirring
PH to 4.93 is adjusted after 4min, is then heated to 45 DEG C by mixed liquor under the conditions of power is 320W, frequency is 74KHz, is surpassed
Sound 41min, is then added enzymolysis agent, under the conditions of temperature is 28 DEG C, digests 5.5h, is finally 4000r/ in revolving speed by extracting solution
Broccoli sprout crude extract is obtained after being centrifuged 30s, suction filtration under conditions of min；
Wherein, mixing extractant is methanol, ethyl alcohol, Victoria C, sodium disulfide and deionized water 0.2:0.5:0.4 in mass ratio:
The mixed solution of 0.05:1.2 composition；Enzymolysis agent and the volume ratio of mixed liquor are 0 .31:1.5；Enzymatic hydrolysis is simultaneously with 190r/min
Rate carry out magnetic agitation；Enzymolysis agent is the mixture that myrosin and radish dry powder 1:0.5 in mass ratio are formed.
S40, the preparation of broccoli sprout extract: broccoli sprout crude extract is extracted 2 times repeatedly using ethyl acetate in S30
Afterwards under the conditions of temperature is 42 DEG C, it is evaporated to the 1/5 of broccoli sprout crude extract volume using Rotary Evaporators, is subsequently placed in jelly
4.2h is lyophilized under the conditions of temperature is -40 DEG C in dry machine, obtains the broccoli sprout extract.Broccoli bud is obtained through detection
The recovery rate of seedling extract is 12.41%.
These are only the preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of extraction process of broccoli sprout extract, characterized in that steps are as follows for the extraction process:
S10, the selection of broccoli sprout: choosing the broccoli sprout sprouted 4 days~6 days, and clear water washing drains, spare；
S20, the processing of broccoli sprout: the broccoli sprout drained in S10 is placed in the baking oven that temperature is 65 DEG C~75 DEG C, baking
10s is irradiated in the UV lamp after pulverizing and sieving after 20min~35min, obtains sprout powder, it is spare；
S30, sprout powder extract: mixing extractant is added in S20 in sprout powder and stirs so that solid-liquid ratio is 1:1.6~2.2
PH is adjusted after mixing 3min~6min, 35 DEG C~55 DEG C is then heated to and extracts, will be obtained after extracting solution centrifugation, suction filtration blue and white
Dish sprout crude extract；
S40, broccoli sprout extract preparation: in S30 broccoli sprout crude extract using ethyl acetate extract 2 times repeatedly after
Under the conditions of temperature is 40 DEG C~44 DEG C, it is evaporated to the 1/6~1/3 of broccoli sprout crude extract volume using Rotary Evaporators, so
It is placed in freeze dryer and is lyophilized, obtain the broccoli sprout extract.
2. extraction process as described in claim 1, characterized in that in S20, the mesh number of the crushing is 100 mesh~200 mesh.
3. extraction process as described in claim 1, characterized in that in S30, the mixing extractant be methanol, ethyl alcohol, Victoria C,
The mixed solution of sodium disulfide and deionized water 0.2:0.5:0.4:0.05:1 in mass ratio~1.5 composition.
4. extraction process as described in claim 1, characterized in that in S30, the pH is adjusted to 4.85~5.05.
5. extraction process as described in claim 1, characterized in that in S30, the extraction specifically: by mixed liquor in function
Under the conditions of rate is 300W~350W, frequency is 65KHz~85KHz, then enzymolysis agent is added, in temperature in ultrasonic 35min~45min
Under the conditions of degree is 25 DEG C~30 DEG C, 4.5h~6.5h is digested.
6. extraction process as claimed in claim 5, characterized in that the enzymolysis agent and the volume ratio of mixed liquor are 0 .25
~0.36:1.5.
7. extraction process as claimed in claim 5, characterized in that the enzymatic hydrolysis is simultaneously with the speed of 160r/min~220r/min
Rate carries out magnetic agitation.
8. extraction process as claimed in claim 5, characterized in that the enzymolysis agent is that myrosin and radish dry powder press quality
The mixture formed than 1:0.5~0.6.
9. extraction process as described in claim 1, characterized in that in S30, the centrifugation is specifically, be 3500r/ in revolving speed
25s~35s is centrifuged under conditions of min~4500r/min.
10. extraction process as described in claim 1, characterized in that in S40, the freeze-drying is specifically, be -38 DEG C in temperature
4.0h~4.5h is lyophilized under the conditions of~-42 DEG C.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514174A (en) * | 2009-02-24 | 2009-08-26 | 黑龙江八一农垦大学 | Method for extracting multifunctional sulforaphane from broccoli sprouting vegetable |
| KR20130040507A (en) * | 2011-10-14 | 2013-04-24 | (주)미스바알텍 | Producing method for broccoli sprouts buds |
| CN104800270A (en) * | 2015-05-20 | 2015-07-29 | 皖南医学院 | Preparation method for broccoli sprout extract capsule |
| CN105246339A (en) * | 2014-04-14 | 2016-01-13 | 比克比欧有限公司 | Method for producing broccoli having increased sulforaphane content and use of broccoli produced therefrom |
| KR20170023512A (en) * | 2015-08-24 | 2017-03-06 | 경상대학교산학협력단 | Composition for improvement of learning and memory function comprising broccoli leaf extract or its fraction as effective component |
-
2019
- 2019-01-18 CN CN201910108244.4A patent/CN109674055A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514174A (en) * | 2009-02-24 | 2009-08-26 | 黑龙江八一农垦大学 | Method for extracting multifunctional sulforaphane from broccoli sprouting vegetable |
| KR20130040507A (en) * | 2011-10-14 | 2013-04-24 | (주)미스바알텍 | Producing method for broccoli sprouts buds |
| CN105246339A (en) * | 2014-04-14 | 2016-01-13 | 比克比欧有限公司 | Method for producing broccoli having increased sulforaphane content and use of broccoli produced therefrom |
| CN104800270A (en) * | 2015-05-20 | 2015-07-29 | 皖南医学院 | Preparation method for broccoli sprout extract capsule |
| KR20170023512A (en) * | 2015-08-24 | 2017-03-06 | 경상대학교산학협력단 | Composition for improvement of learning and memory function comprising broccoli leaf extract or its fraction as effective component |
Non-Patent Citations (1)
| Title |
|---|
| 王天珩等: "西兰花芽苗提取物提取工艺优化研究", 《医学理论与实践》 * |
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