CN109668995A - A kind of quality standard detecting method of pearl ganoderma lucidum slice - Google Patents
A kind of quality standard detecting method of pearl ganoderma lucidum slice Download PDFInfo
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- CN109668995A CN109668995A CN201910158754.2A CN201910158754A CN109668995A CN 109668995 A CN109668995 A CN 109668995A CN 201910158754 A CN201910158754 A CN 201910158754A CN 109668995 A CN109668995 A CN 109668995A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
The invention discloses a kind of quality standard detecting methods of pearl ganoderma lucidum slice, the inspection of identification, pearl ganoderma lucidum slice including pearl ganoderma lucidum slice, pearl ganoderma lucidum slice related substances assay, in quality standard detecting method of the invention, increase identification and the assay of monarch drug in a prescription glossy ganoderma extract, more effectively to control product quality, the thin layer that this experiment establishes monarch drug in a prescription glossy ganoderma extract in pearl ganoderma lucidum slice identifies and the high performance liquid chromatography content determination of ganoderic acid A, provides scientific basis for the control of pearl ganoderma lucidum tablet quality.
Description
Technical field
The present invention relates to Chinese medicine detection technique field more particularly to a kind of quality standard detecting methods of pearl ganoderma lucidum slice.
Background technique
Pearl ganoderma lucidum slice is as a kind of prescription medicine, by glossy ganoderma extract, the fruit of glossy privet, Radix Curcumae, rhizoma cyperi, eclipta, dried orange peel, pearl
Layer powder is prepared, and has mental-tranquilization, and nourishing liver and kidney effect is widely used in chronic hepatitis, neurasthenia, dizzy mistake
The diseases such as dormancy, gastroenteritic ulcer, chronic bronchitis, coronary heart disease.
The quality standard of existing pearl ganoderma lucidum slice is to carry out matter to pearl ganoderma lucidum slice by character, identification, inspection item
Amount control, the identification of no monarch drug in a prescription glossy ganoderma extract and assay.More effectively to control product quality, this experiment establishes pearl
The thin layer of monarch drug in a prescription glossy ganoderma extract identifies the high performance liquid chromatography content determination with ganoderic acid A in ganoderma lucidum slice, is pearl ganoderma lucidum slice
Quality control provides scientific basis.
Summary of the invention
The object of the invention is exactly to provide a kind of pearl to solve quality standard to the deficiency and defect of monarch drug in a prescription detection control
The quality standard detecting method of ganoderma lucidum slice.
The present invention is achieved by the following technical solutions:
A kind of quality standard detecting method of pearl ganoderma lucidum slice, comprising the following steps:
(1) identification of pearl ganoderma lucidum slice；
(2) inspection of pearl ganoderma lucidum slice；
(3) assay of pearl ganoderma lucidum slice related substances.
The discrimination method the following steps are included:
(1) this product 2 are taken, sugar-coat is removed, it is finely ground, add water 10ml, boil, let cool, filters, take filter residue to set under microscope and see
It examines, answers visible numerous irregular, strips or round block piece, it is translucent, have fine and closely woven wavy texture, is dripped at coverslip edge
Add dilute hydrochloric acid, a large amount of bubbles should be generated；
(2) this product 10 are taken, sugar-coat, finely ground, each 10ml secondary with extracted by ether are removed, filtration takes residue to volatilize second
Ether adds water 10ml to impregnate a few hours, and filtration takes filtrate point on filter paper for chromatography, is unfolded with solvent, takes out, dries, and sprays with indenes
Triketone test solution dries 10 minutes at 80 DEG C, should show bluish violet spot；
(3) it takes and identifies 2 residues being lauched after extracting, 0.5% ethanol solution hydrochloride 20ml is added, temperature leaching 30 minutes is put
Cold, filtration, filtrate is evaporated, and residue adds dilute hydrochloric acid 10ml to make to dissolve, and filtration takes filtrate 1ml, adds few drops of test solution of mercuric potassium iodide, answer
Generate faint yellow muddiness；Filtrate 1ml is taken, is added few drops of bismuth potassium iodide test solution, Ying Shengcheng yellow is muddy；Filtrate 1ml is taken, silico-tungstic acid is added
Few drops of test solution, Ying Shengcheng lark precipitating；
(4) this product 5 are taken, it is finely ground, add ethyl acetate 15ml, be ultrasonically treated 30min, filtration, filtrate is evaporated, and residue adds first
Alcohol 0.5ml makes to dissolve, as test solution；Ganoderic acid A reference substance is taken, adds methanol 1ml the is made solution containing 1mg, as right
According to product solution；According to thin-layered chromatography, above-mentioned each 10 μ l of 2 kinds of solution is drawn, is put respectively on same silica GF254 lamellae, with
Solvent expansion, dries, sets and inspect under ultraviolet lamp 254nm, should be with reference substance chromatography in corresponding positions wherein in sample chromatogram
It sets, shows identical blackening；Spray is with 0.5% vanillin-sulfuric acid solution, in 105 DEG C of laser heating 10-15min to clear spot,
Again in sample chromatogram, the spot of same color should be shown on position corresponding with reference substance chromatography.
The solvent identified in item 2 is n-butanol, acetic acid, ethyl alcohol, 4:1:1:2 is mixed water by volume.
The petroleum ether and Ethyl formate, formic acid, chloroform that the solvent identified in item 4 is 60-90 DEG C press body
Product is mixed than 4:5:1:1.
The inspection method the following steps are included:
(1) disintegration time limited: disintegration time limit test inspection is shone, each should all be disintegrated in 60 minutes；
(2) microbial limit: shining non-sterile product limit test of microbe, and aerobic bacteria sum should be less than 1000cfu/g；It is mould
Bacterium and yeast count sum should be less than 50cfu/g；Escherichia coli and detection of Salmonella must not be detected；Bile tolerance gram-negative bacteria is answered small
In 200cfu/g.
The assay the following steps are included:
(1) chromatographic condition and system suitability measure: with Diamonsil Plus 5um C18,250*4.6mm chromatographic column,
Using acetonitrile as mobile phase A, using 0.1% phosphoric acid solution as Mobile phase B, gradient elution is carried out；Detection wavelength is 254nm, theoretical plate
Number is calculated by the peak ganoderic acid A should be not less than 20000；
(2) preparation of reference substance solution: precision weighs ganoderic acid A reference substance, adds methanol that every 1ml is made containing the molten of 10 μ g
Liquid to get；
(3) preparation of test solution: taking this product 20, removes coating, finely ground, takes 0.5g, accurately weighed, sets tool plug cone
In shape bottle, methanol 50ml is added in precision, and weighed weight is taken out after ultrasonic treatment, lets cool, then weighed weight, supplied and subtracted with methanol
The weight of mistake, shakes up, filtration, take subsequent filtrate to get；
(4) measuring method: it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, liquid chromatograph is injected, is surveyed
Determine to get every A containing ganoderic acid of this product should not be less than 0.16mg.
Gradient elution is carried out as follows in the detection method step 1:
The power being ultrasonically treated in the detection method step 3 is 300W, and frequency 40kHz, the time is 30 minutes.
The invention has the advantages that
In quality standard detecting method of the invention, identification and the assay of monarch drug in a prescription glossy ganoderma extract are increased, more to have
Effect ground control product quality, the thin layer that this experiment establishes monarch drug in a prescription glossy ganoderma extract in pearl ganoderma lucidum slice identifies and the height of ganoderic acid A
Effect liquid phase chromatogram content determination provides scientific basis for the control of pearl ganoderma lucidum tablet quality.
Specific embodiment
Below in conjunction with specific example, technical scheme is described further:
A kind of quality standard detecting method of pearl ganoderma lucidum slice, comprising the following steps:
(1) identification of pearl ganoderma lucidum slice；
(2) inspection of pearl ganoderma lucidum slice；
(3) assay of pearl ganoderma lucidum slice related substances.
Detect pearl ganoderma lucidum tablet recipe used, constitutive material are as follows: glossy ganoderma extract 50g, fruit of glossy privet 50g, Radix Curcumae 100g are fragrant
Attached 100g, eclipta 50g, dried orange peel 100g, nacreous layer powder 25g.
The discrimination method the following steps are included:
(1) this product 2 are taken, sugar-coat is removed, it is finely ground, add water 10ml, boil, let cool, filters, take filter residue to set under microscope and see
It examines, answers visible numerous irregular, strips or round block piece, it is translucent, have fine and closely woven wavy texture, is dripped at coverslip edge
Add dilute hydrochloric acid, generates a large amount of bubbles；
(2) this product 10 are taken, sugar-coat, finely ground, each 10ml secondary with extracted by ether are removed, filtration takes residue to volatilize second
Ether adds water 10ml to impregnate a few hours, and filtration takes filtrate point on filter paper for chromatography, is unfolded with solvent, takes out, dries, and sprays with indenes
Triketone test solution dries 10 minutes at 80 DEG C, shows bluish violet spot；
(3) it takes and identifies 2 residues being lauched after extracting, 0.5% ethanol solution hydrochloride 20ml is added, temperature leaching 30 minutes is put
Cold, filtration, filtrate is evaporated, and residue adds dilute hydrochloric acid 10ml to make to dissolve, and filtration takes filtrate 1ml, adds few drops of test solution of mercuric potassium iodide, raw
At faint yellow muddiness；Filtrate 1ml is taken, is added few drops of bismuth potassium iodide test solution, it is muddy to generate yellow；Filtrate 1ml is taken, silico-tungstic acid test solution is added
Few drops, generate lark precipitating；
(4) this product 5 are taken, it is finely ground, add ethyl acetate 15ml, be ultrasonically treated 30min, filtration, filtrate is evaporated, and residue adds first
Alcohol 0.5ml makes to dissolve, as test solution；Ganoderic acid A reference substance is taken, adds methanol 1ml the is made solution containing 1mg, as right
According to product solution；According to thin-layered chromatography, above-mentioned each 10 μ l of 2 kinds of solution is drawn, is put respectively on same silica GF254 lamellae, with
Solvent expansion, dries, sets and inspect under ultraviolet lamp 254nm, wherein in sample chromatogram, with reference substance chromatography in corresponding position
On, show identical blackening；Spray is with 0.5% vanillin-sulfuric acid solution, in 105 DEG C of laser heating 10-15min to clear spot, then
In sample chromatogram, on position corresponding with reference substance chromatography, the spot of same color is shown.
Wherein the solvent in identification item 2 is n-butanol, acetic acid, ethyl alcohol, 4:1:1:2 is mixed water by volume.
Wherein identify the solvent in item 4 and presses body for 60-90 DEG C of petroleum ether and Ethyl formate, formic acid, chloroform
Product is mixed than 4:5:1:1.
The inspection method the following steps are included:
(1) disintegration time limited: disintegration time limit test inspection is shone, each is all disintegrated in 60 minutes；
(2) microbial limit: shining non-sterile product limit test of microbe, and aerobic bacteria sum is less than 1000cfu/g；Mould
It is less than 50cfu/g with yeast count sum；Escherichia coli and detection of Salmonella is not detected；Bile tolerance gram-negative bacteria is less than
200cfu/g。
The assay the following steps are included:
(1) chromatographic condition and system suitability measure: with Diamonsil Plus 5um C18,250*4.6mm chromatographic column,
Using acetonitrile as mobile phase A, using 0.1% phosphoric acid solution as Mobile phase B, according to the form below carries out gradient elution；Detection wavelength is 254nm,
Number of theoretical plate is calculated by the peak ganoderic acid A is not less than 20000；
(2) preparation of reference substance solution: precision weighs ganoderic acid A reference substance, adds methanol that every 1ml is made containing the molten of 10 μ g
Liquid to get；
(3) preparation of test solution: taking this product 20, removes coating, finely ground, takes 0.5g, accurately weighed, sets tool plug cone
In shape bottle, methanol 50ml is added in precision, and weighed weight with power is 300W, frequency 40kHz, time are to be surpassed for 30 minutes
Take out, let cool after sonication, then weighed weight, the weight of less loss is supplied with methanol, is shaken up, filter, take subsequent filtrate to get；
(4) measuring method: it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, liquid chromatograph is injected, is surveyed
Determine to get every A containing ganoderic acid of this product is no less than 0.16mg.
Claims (8)
1. a kind of quality standard detecting method of pearl ganoderma lucidum slice, which comprises the following steps:
(1) identification of pearl ganoderma lucidum slice；
(2) inspection of pearl ganoderma lucidum slice；
(3) assay of pearl ganoderma lucidum slice related substances.
2. the quality standard detecting method of pearl ganoderma lucidum slice according to claim 1, which is characterized in that the discrimination method
The following steps are included:
(1) this product 2 are taken, sugar-coat is removed, it is finely ground, add water 10ml, boil, let cool, filters, filter residue is taken to set microscopically observation,
Visible numerous irregular, strips or round block piece are answered, it is translucent, have fine and closely woven wavy texture, is added dropwise at coverslip edge
Dilute hydrochloric acid should generate a large amount of bubbles；
(2) this product 10 are taken, sugar-coat, finely ground, each 10ml secondary with extracted by ether are removed, filtration takes residue to volatilize ether,
Water 10ml is added to impregnate a few hours, filtration takes filtrate point on filter paper for chromatography, is unfolded with solvent, takes out, dries, and sprays with indenes three
Ketone test solution dries 10 minutes at 80 DEG C, should show bluish violet spot；
(3) it takes and identifies 2 residues being lauched after extracting, 0.5% ethanol solution hydrochloride 20ml is added, temperature leaching 30 minutes lets cool, filters
It crosses, filtrate is evaporated, and residue adds dilute hydrochloric acid 10ml to make to dissolve, and filtration takes filtrate 1ml, adds few drops of test solution of mercuric potassium iodide, should generate light
Yellow is muddy；Filtrate 1ml is taken, is added few drops of bismuth potassium iodide test solution, Ying Shengcheng yellow is muddy；Filtrate 1ml is taken, silico-tungstic acid test solution number is added
Drop, Ying Shengcheng lark precipitating；
(4) this product 5 are taken, it is finely ground, add ethyl acetate 15ml, be ultrasonically treated 30min, filtration, filtrate is evaporated, and residue adds methanol
0.5ml makes to dissolve, as test solution；Ganoderic acid A reference substance is taken, adds methanol 1ml the is made solution containing 1mg, as control
Product solution；According to thin-layered chromatography, above-mentioned each 10 μ l of 2 kinds of solution is drawn, is put respectively on same silica GF254 lamellae, with exhibition
Agent expansion is opened, is dried, is set and inspected under ultraviolet lamp 254nm, it, should be with reference substance chromatography in corresponding position wherein in sample chromatogram
On, show identical blackening；Spray is with 0.5% vanillin-sulfuric acid solution, in 105 DEG C of laser heating 10-15min to clear spot, then
In sample chromatogram, the spot of same color should be shown on position corresponding with reference substance chromatography.
3. the quality standard detecting method of pearl ganoderma lucidum slice according to claim 2, which is characterized in that the identification item 2
In solvent be n-butanol, acetic acid, ethyl alcohol, 4:1:1:2 is mixed water by volume.
4. the quality standard detecting method of pearl ganoderma lucidum slice according to claim 2, which is characterized in that the identification item 4
In solvent be 60-90 DEG C petroleum ether and Ethyl formate, formic acid, chloroform 4:5:1:1 is mixed by volume.
5. the quality standard detecting method of pearl ganoderma lucidum slice according to claim 1, which is characterized in that the inspection method
The following steps are included:
(1) disintegration time limited: disintegration time limit test inspection is shone, each should all be disintegrated in 60 minutes；
(2) microbial limit: shining non-sterile product limit test of microbe, and aerobic bacteria sum should be less than 1000cfu/g；Mould and
Yeast count sum should be less than 50cfu/g；Escherichia coli and detection of Salmonella must not be detected；Bile tolerance gram-negative bacteria should be less than
200cfu/g。
6. the quality standard detecting method of pearl ganoderma lucidum slice according to claim 1, which is characterized in that the assay
The following steps are included:
(1) chromatographic condition and system suitability measure: with Diamonsil Plus 5um C18,250*4.6mm chromatographic column, with second
Nitrile is mobile phase A, using 0.1% phosphoric acid solution as Mobile phase B, carries out gradient elution；Detection wavelength is 254nm, and number of theoretical plate is pressed
The peak ganoderic acid A, which calculates, should be not less than 20000；
(2) preparation of reference substance solution: precision weighs ganoderic acid A reference substance, adds methanol that the solution that every 1ml contains 10 μ g is made, i.e.,
?；
(3) preparation of test solution: taking this product 20, removes coating, finely ground, takes 0.5g, accurately weighed, sets stuffed conical flask
In, methanol 50ml is added in precision, and weighed weight is taken out after ultrasonic treatment, lets cool, then weighed weight, supply less loss with methanol
Weight shakes up, filtration, take subsequent filtrate to get；
(4) measuring method: it is accurate respectively to draw reference substance solution and each 20 μ l of test solution, liquid chromatograph is injected, is measured, i.e.,
, every A containing ganoderic acid of this product should not be less than 0.16mg.
7. the quality standard detecting method of pearl ganoderma lucidum slice according to claim 6, which is characterized in that in the step 1
Gradient elution is carried out as follows:
8. the quality standard detecting method of pearl ganoderma lucidum slice according to claim 6, which is characterized in that in the step 3
The power of ultrasonic treatment is 300W, and frequency 40kHz, the time is 30 minutes.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110772484A (en) * | 2019-11-12 | 2020-02-11 | 安徽协和成制药有限公司 | Fried sophora flower formula particle and preparation method and quality standard detection method thereof |
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2019
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Denomination of invention: A quality standard test method for pearl ganoderma lucidum tablets Effective date of registration: 20230228 Granted publication date: 20210416 Pledgee: Bozhou Shouxin Financing Guarantee Co.,Ltd. Pledgor: ANHUI XIEHECHENG PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980033645 |
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Date of cancellation: 20230725 Granted publication date: 20210416 Pledgee: Bozhou Shouxin Financing Guarantee Co.,Ltd. Pledgor: ANHUI XIEHECHENG PHARMACEUTICAL Co.,Ltd. Registration number: Y2023980033645 |