CN109668866B - Preparation and detection method of fluorescent probe for detecting iodide ions in water environment - Google Patents
Preparation and detection method of fluorescent probe for detecting iodide ions in water environment Download PDFInfo
- Publication number
- CN109668866B CN109668866B CN201910068560.3A CN201910068560A CN109668866B CN 109668866 B CN109668866 B CN 109668866B CN 201910068560 A CN201910068560 A CN 201910068560A CN 109668866 B CN109668866 B CN 109668866B
- Authority
- CN
- China
- Prior art keywords
- iodide ions
- phosphorus oxide
- oxide quantum
- quantum dot
- fluorescent probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/70—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing phosphorus
- C09K11/701—Chalcogenides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
Abstract
The invention provides a preparation method of a fluorescent probe for detecting iodide ions in a water sample, which is characterized by comprising the following steps: step 1: introducing oxygen into the reactorMixing phosphorus dioxide solid, sodium hydroxide solid and N-methylpyrrolidone, then carrying out ultrasonic treatment at room temperature, putting the reaction liquid after ultrasonic treatment in an oil bath pot, and preparing phosphorus oxide quantum dots by a solvothermal method; centrifuging the reaction solution to remove precipitates, taking supernate, purifying by a dialysis method, concentrating and freeze-drying the dialysate to obtain a blue-green fluorescent phosphorus oxide quantum dot solid material; step 2: dissolving the blue-green fluorescent phosphorus oxide quantum dot solid material in deionized water to obtain a phosphorus oxide quantum dot aqueous solution, and mixing the phosphorus oxide quantum dot aqueous solution and Ag+Mixing the aqueous solution to obtain Ag+The mediated phosphorus oxide quantum dot probe is a fluorescent probe for detecting iodide ions in a water sample. The fluorescent probe obtained by the invention can be used for rapidly detecting iodide ions, and has better selectivity on iodide ions in different water samples.
Description
Technical Field
The invention relates to a preparation method of blue-green fluorescent phosphorus oxide quantum dots and a method for detecting iodide ions, and belongs to the technical field of nano material preparation and application.
Background
Iodine is extremely important to the life of animals and plants. In higher mammals, iodine is concentrated in the thyroid gland in the form of iodinated amino acids, and has the effects of promoting sugar and fat metabolism, regulating water and salt metabolism, promoting absorption and utilization of vitamins, enhancing enzyme activity, promoting growth and development, etc. Deficiency of iodine can cause goiter, seriously affect normal development of children, and is manifested by mental retardation, deafness, dumb, dwarfism, and excessive iodine intake can also harm human health, and can cause a series of hypermetabolic syndromes of systems such as nervous system, circulatory system, digestive system, cardiovascular system, etc., and hyperexcitability symptoms and eye symptoms.
The existing methods for detecting iodide ions include colorimetry, titration, electrochemistry, chromatography and the like, and although the methods can realize sensitive and selective detection of iodide ions, most of the methods are complex, time-consuming, labor-consuming and have a plurality of interference factors. The phosphorus oxide quantum dot fluorescent probe has excellent luminous performance; high light stability; continuous absorption spectrum (from ultraviolet to visible); meanwhile, the method has the advantages of high selectivity and sensitivity, easy operability of a nondestructive analysis and detection system and the like, and has a great potential application prospect in the fields of environment, chemistry, biology, medicine and the like.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a fluorescent probe for detecting iodide ions in a water sample, and also provides a preparation method and application thereof. The fluorescent probe can be used for rapidly detecting iodide ions, has good selectivity on iodide ions in different water samples, and has little interference on detection by other common anions.
In order to achieve the above object, the present invention provides a method for preparing a fluorescent probe for detecting iodide ions in a water sample, comprising:
step 1: according to the feeding ratio of (0.8-1) mg: (0.8-1) mg: 1mL, mixing phosphorus pentoxide solid, sodium hydroxide solid and N-methyl pyrrolidone, and then carrying out ultrasonic treatment for 25-35 minutes at room temperature; heating the reaction liquid after ultrasonic treatment by adopting an oil bath pot, and preparing phosphorus oxide quantum dots by a solvothermal method; centrifuging the reaction solution to remove precipitates, taking supernate, purifying by a dialysis method, concentrating and freeze-drying the dialysate to obtain a fluorescent phosphorus oxide quantum dot solid material;
step 2: dissolving the fluorescent phosphorus oxide quantum dot solid material in deionized water to obtain a phosphorus oxide quantum dot aqueous solution, and mixing the phosphorus oxide quantum dot aqueous solution and Ag+Mixing the aqueous solution to obtain Ag+The mediated phosphorus oxide quantum dot probe is a fluorescent probe for detecting iodide ions in a water sample.
Preferably, the temperature of the oil bath in the step 1 is set to be 160-185 ℃.
Preferably, the stirring speed is 360-400 r/min, and the reaction time is 8-12 hours.
Preferably, the phosphorus oxide quantum dots and Ag+The feeding mass ratio of (A) to (B) is 1: 2500-1: 800.
Preferably, said Ag is+The concentration of the aqueous solution is 0.01-0.05 mol/L.
The invention also provides the fluorescent probe prepared by the preparation method and used for detecting the iodide ions in the water sample.
The invention also provides application of the fluorescent probe for detecting the iodide ions in the water sample in detecting the iodide ions in the water sample.
The invention also provides a method for detecting iodide ions in a water sample, which is characterized by comprising the following steps:
step 1: firstly, mixing a buffer solution with the fluorescent probe for detecting iodide ions in a water sample, adding iodide ions with different concentrations after mixing, recording a fluorescence spectrum after stirring, and obtaining a relation graph of fluorescence intensity and iodide ion concentration through linear fitting;
step 2: and mixing the buffer solution with a fluorescent probe for detecting iodide ions in a water sample, adding the mixture into a sample to be detected, stirring the mixture for reaction, measuring the fluorescence intensity, and obtaining the concentration of the iodide ions in the sample to be detected according to the fluorescence intensity.
The fluorescent probe can be used for detecting iodide ions in a water sample in a fluorescence quenching mode.
Compared with the prior art, the method has the following beneficial effects:
1. the fluorescent probe is a blue-green fluorescent phosphorus oxide quantum dot (lambda)em490nm), the quantum dot pair to Ag+Insensitivity, Ag+The mediated phosphorus oxide quantum dot solution still keeps stronger fluorescence intensity, and after the iodine ion solutions with different concentrations are added, the fluorescence of the quantum dots is gradually quenched.
2. The fluorescent probe only acts with iodide ions, and other common anions have little influence on the fluorescence, so that the fluorescent probe has good selectivity and specificity.
3. The fluorescent probe is easy to dissolve in water, convenient for detection of iodide ions in various water environments, simple and feasible in preparation process, short in reaction time, strong in signal response and good in reproducibility.
4. The fluorescent probe obtained by the invention can be used for rapidly detecting iodide ions, has good selectivity on iodide ions in different water samples, has little detection interference on the fluorescent probe by other common anions, and has good selectivity, simple and convenient operation, high sensitivity and good reproducibility.
Drawings
FIG. 1 is a schematic representation of the working principle of the fluorescent probe of the present invention.
FIG. 2 shows the addition of Ag to the phosphorus oxide quantum dot solution obtained in example 1+And (3) front and back ultraviolet absorption spectrum change diagrams.
FIG. 3 is a transmission electron micrograph (a) of the fluorescent probe obtained in example 1, and shows UV absorption and fluorescence emission spectra of a phosphorus oxide quantum dot aqueous solution sample. The inset (left) is a photo of a phosphorus oxide quantum dot aqueous solution sample, which is light yellow; the inset (right) is a fluorescent photograph of the phosphorus oxide quantum dots under 365nm wavelength ultraviolet lamp illumination (b).
FIG. 4 is a graph (a) showing response time of the fluorescent probe of the present invention to iodide ion signals, a graph (b) showing fluorescence quenching, and a graph (c) showing operation curves under the conditions of example 2.
FIG. 5 is a graph of selectivity of the present invention for other common anions under the conditions of example 3.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
FIG. 1 is a schematic representation of the working principle of the fluorescent probe of the present invention. Firstly, a phosphorus oxide quantum dot is prepared by a solvothermal method, and the emission wavelength of the quantum dot is 490nm (the excitation wavelength is 400nm) and shows blue-green fluorescence. The quantum dot pair Ag+Insensitivity, Ag+The mediated phosphorus oxide quantum dot solution still keeps stronger fluorescence intensity, under the existence of iodide ions, the iodide ions and silver ions react to generate silver iodide, the absorption spectrum of the silver iodide is overlapped with the excitation spectrum of the quantum dots, a fluorescence inner filtering effect is generated between the absorption spectrum and the excitation spectrum, and further the fluorescence of the quantum dots is quenched.
Example 1
(1) Preparation of novel blue-green phosphorus oxide quantum dots
Dissolving 8mg of phosphorus pentoxide solid in 10mL of N-methylpyrrolidone at room temperature, then adding 8mg of sodium hydroxide solid, mixing, and carrying out ultrasonic treatment at room temperature for 30 minutes at an ultrasonic frequency of 40KHZ and an electric power of 100W; heating the reaction liquid after ultrasonic treatment by adopting an oil bath pot, preparing the phosphorus oxide quantum dots by a solvothermal method, setting the oil bath temperature at 160 ℃, keeping a strong stirring speed, rotating at 360 revolutions per minute, and reacting for 8 hours; after the reaction is finished, taking out the reaction solution, centrifuging at a high speed (8000 rpm), removing the precipitate, and taking the supernatant; and (4) taking the supernatant for dialysis and purification, wherein the total dialysis time is 2 days. The dialysis bag has a specification of 2kD, and the distilled water is changed every 4 hours to achieve good dialysis effect. And collecting the dialyzate, performing rotary evaporation concentration, and finally performing freeze drying to obtain the blue-green fluorescent phosphorus oxide quantum dot solid material.
(2) Fluorescent probe for detecting iodide ions in prepared water sample
Dissolving a certain amount of blue-green fluorescent phosphorus oxide quantum dot solid material in a certain volume of deionized water to obtain a phosphorus oxide quantum dot aqueous solution (1 × 10)-2mg·mL-1) Phosphorus oxide quantum dot aqueous solution and 0.02M Ag+(AgNO3) Mixing the aqueous solution, the phosphorus oxide quantum dots and Ag+The feeding ratio (mass ratio) of 1: 1080 to obtain Ag+And (3) refrigerating the mediated phosphorus oxide quantum dot probe for later use. To verify whether the mediated phosphorus oxide quantum dot fluorescent probe was successfully synthesized, we added Ag to separate quantum dot solutions+The quantum dot solution is subjected to ultraviolet absorption spectrum test, and Ag is added as shown in figure 2+The ultraviolet absorption of the quantum dots generates a certain degree of blue shift, which shows that the surface state of the quantum dots is changed due to the introduction of silver ions, and indirectly proves that Ag is successfully synthesized+Mediated phosphorus oxide quantum dot probes.
And (3) performing characterization to obtain a transmission electron microscope image in a figure 3(a) and an ultraviolet absorption and fluorescence emission spectrogram of a phosphorus oxide quantum dot aqueous solution sample in a figure 3 (b). As shown in fig. 3(a), it can be observed that the average particle diameter of the quantum dots is 6.3 ± 1.2nm, and the nanoparticles show a better monodispersed state, indicating that the quantum dots are not aggregated and have better stability. As shown in FIG. 3(b), the inset (left) is a photograph of the phosphorus oxide quantum dot aqueous solution sample, which is light yellow; the inset (right) is a fluorescent photograph of the phosphorus oxide quantum dots under 365nm wavelength ultraviolet lamp illumination.
Example 2
Phosphate Buffer Solution (PBS) of pH6.0 was prepared in advance with deionized water for use, 2.0mL of buffer solution (10mM) was added to a 10mM quartz cuvette, and 10. mu.L of the prepared Ag solution of example 1 was accurately transferred+Mediated phosphorus oxide quantum dot in water (1X 10)-2mg·mL-1) Dispersing in buffer solution in cuvette, stirring rapidly, placing in fluorometer to record fluorescence intensity, and recording as F0Adding an aqueous solution of iodide ions (0.093mM) into the solution, stirring for a certain time until the fluorescence intensity does not change any more, and recording the change of the fluorescence intensity at different times, as shown in FIG. 4(a), the fluorescence intensity of the quantum dots tends to be stable within 60s, which indicates that the time period for the fluorescence probe to detect the iodide ions is 1 minute, and simultaneously recording the fluorescence intensities of the quantum dots at different concentrations of the iodide ions (0.013mM, 0.021mM, 0.029mM, 0.037mM, 0.045mM, 0.053mM, 0.061mM, 0.069mM, 0.085mM, and 0.093mM), as shown in FIG. 4(b), the fluorescence intensity of the quantum dots gradually decreases with the increase of the iodide ion concentration. In the range of 0.013-0.093mM of iodide ion, Ag+Relative fluorescence intensity F/F of mediated phosphorus oxide quantum dot probe0(as Y-axis) and iodide ion concentration (as X-axis) to obtain a good linear relationship (R)20.99029), a working curve for detecting iodide ions is obtained, as shown in fig. 4 (c).
Example 3
F was prepared in an amount of 0.02mol/L under the conditions of example 1-,Cl-,PO4 3-,BO3 3-,NO3 2-,SO4 2-,NO2 -,Cr2O7 2-(NaF，NaCl，Na3PO4，H3BO3，Na2SO4，NaNO2，K2Cr2O7) PBS buffer pH6.0, each2mL of the suspension was added with 10. mu.L (1X 10)-2mg·mL-1) Ag of (A)+The mediated phosphorus oxide quantum dot in water solution was stirred and the fluorescence spectrum was recorded, and compared with the fluorescence intensity under the action of iodide ions in example 1 to obtain FIG. 5, as shown in FIG. 5(a), F-,Cl-,PO4 2-,BO3 3-,NO3 2-,SO4 2-,NO2 -,Cr2O7 2-Plasma anion is even at 200 times I-No fluorescence quenching ability is shown under the condition of concentration level, only Br-shows little quenching ability, and the description shows that I-For Ag+The mediated PO QDs have good fluorescence quenching capacity, and we also have good fluorescence quenching capacity on I-Test with other coexisting anionic interferents, and I of the test-In contrast, these interfering anions are present in higher concentrations than I-200 times of solution, in the presence of these anionic interferents, Ag+Mediated PO QDs as I-Fluorescent probe pair I-Has high selectivity, and the influence of coexisting anion interferent on the optical response of the fluorescent probe is negligible. In FIG. 5(b), bar a is represented by F-,Cl-,PO4 2-,BO3 3-,NO3 2-,SO4 2-,NO2 -,Cr2O7 2-(the concentration of each anion solution was 0.02mol/L) response to a fluorescent probe in the coexistence of anions; bar b represents the fluorescent probe in isolation I-(0.093mM) fluorescence response intensity in the presence of; bar c represents I-(0.093mM) and other anions F-,Cl-,PO4 2-,BO3 3-,NO3 2-,SO4 2-,NO2 -,Cr2O7 2-(concentration of 0.02mol/L) under the coexistence condition. The inset is a corresponding fluorescent photograph under uv light.
Claims (7)
1. A preparation method of a fluorescent probe for detecting iodide ions in a water sample is characterized by comprising the following steps:
step 1: according to the feeding ratio of (0.8-1) mg: (0.8-1) mg: 1mL, mixing phosphorus pentoxide solid, sodium hydroxide solid and N-methyl pyrrolidone, and then carrying out ultrasonic treatment for 25-35 minutes at room temperature; placing the reaction liquid after ultrasonic treatment in an oil bath pan, and preparing phosphorus oxide quantum dots by a solvothermal method; centrifuging the reaction solution to remove precipitates, taking supernate, purifying by a dialysis method, concentrating and freeze-drying the dialysate to obtain a blue-green fluorescent phosphorus oxide quantum dot solid material;
step 2: dissolving the blue-green fluorescent phosphorus oxide quantum dot solid material in deionized water to obtain a phosphorus oxide quantum dot aqueous solution, and mixing the phosphorus oxide quantum dot aqueous solution and Ag+Mixing the aqueous solution to obtain Ag+The mediated phosphorus oxide quantum dot probe is a fluorescent probe for detecting iodide ions in a water sample.
2. The method for preparing a fluorescent probe for detecting iodide ions in a water sample according to claim 1, wherein the temperature of the oil bath in the step 1 is set to 160-185 ℃.
3. The method of claim 1, wherein the phosphorus oxide quantum dots and Ag are doped with a fluorescent material+The feeding mass ratio of (A) to (B) is 1: 2500-1: 800.
4. The method of claim 1, wherein the Ag is a silver, or a combination thereof+The concentration of the aqueous solution is 0.01-0.05 mol/L.
5. A fluorescent probe prepared by the preparation method of any one of claims 1 to 4 for detecting iodide ions in a water sample.
6. Use of the fluorescent probe for detection of iodide ions in a water sample according to claim 5 for detecting iodide ions in a water sample.
7. A method for detecting iodide ions in a water sample, comprising:
step 1: firstly, mixing a buffer solution with the fluorescent probe for detecting iodide ions in a water sample according to claim 5, adding the iodide ions with different concentrations after mixing, recording a fluorescence spectrum after stirring, and obtaining a relation graph of fluorescence intensity and iodide ion concentration through linear fitting;
step 2: and mixing the buffer solution with a fluorescent probe for detecting iodide ions in a water sample, adding the mixture into a sample to be detected, stirring the mixture for reaction, measuring the fluorescence intensity, and obtaining the concentration of the iodide ions in the sample to be detected according to the fluorescence intensity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910068560.3A CN109668866B (en) | 2019-01-24 | 2019-01-24 | Preparation and detection method of fluorescent probe for detecting iodide ions in water environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910068560.3A CN109668866B (en) | 2019-01-24 | 2019-01-24 | Preparation and detection method of fluorescent probe for detecting iodide ions in water environment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109668866A CN109668866A (en) | 2019-04-23 |
| CN109668866B true CN109668866B (en) | 2021-04-27 |
Family
ID=66149720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910068560.3A Active CN109668866B (en) | 2019-01-24 | 2019-01-24 | Preparation and detection method of fluorescent probe for detecting iodide ions in water environment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109668866B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111849481A (en) * | 2020-07-28 | 2020-10-30 | 上海应用技术大学 | Non-metal fluorescent nano material and preparation method thereof |
| CN114752373B (en) * | 2022-03-23 | 2024-01-23 | 武汉工程大学 | Application of dysprosium metal organic framework material in fluorescence detection of iodide ions in water body |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104914083B (en) * | 2015-06-14 | 2017-06-16 | 湖南科技大学 | A kind of fluorescence silver nanoclusters detect I simultaneously‑And Br‑Method and utilization |
| CN109081319B (en) * | 2018-10-19 | 2022-02-11 | 上海应用技术大学 | Blue-green fluorescent phosphorus oxide quantum dot material, and preparation method and application thereof |
-
2019
- 2019-01-24 CN CN201910068560.3A patent/CN109668866B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN109668866A (en) | 2019-04-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Penttilä | Properties and reconstitution of a cytochrome oxidase deficient in subunit III | |
| Yang et al. | A Eu3+-inspired fluorescent carbon nanodot probe for the sensitive visualization of anthrax biomarker by integrating EDTA chelation | |
| CN109668866B (en) | Preparation and detection method of fluorescent probe for detecting iodide ions in water environment | |
| Yang et al. | Polyethyleneimine-functionalized carbon dots as a fluorescent probe for doxorubicin hydrochloride by an inner filter effect | |
| Lin et al. | Role of novel silicon nanoparticles in luminescence detection of a family of antibiotics | |
| Wu et al. | Ratiometric fluorescence sensor based on europium-grafted ZnO quantum dots for visual and colorimetric detection of tetracycline | |
| Li et al. | A photoluminescence “switch-on” nanosensor composed of nitrogen and sulphur co-doped carbon dots and gold nanoparticles for discriminative detection of glutathione | |
| Liu et al. | Oxidation etching induced dual-signal response of carbon dots/silver nanoparticles system for ratiometric optical sensing of H2O2 and H2O2-related bioanalysis | |
| CN105928914A (en) | Hydrogen sulfide detection sensor, preparation method thereof, quantitative detection method of hydrogen sulfide, and qualitative detection method of hydrogen sulfide in cells | |
| CN111286324A (en) | Fluorescent probe for detecting hypochlorite in water environment and preparation method and application thereof | |
| CN113295741A (en) | Method for constructing photoelectric photochromic visual sensor for simultaneously detecting multiple organic pollutants | |
| Liu et al. | A facile fluorescent sensing strategy for determination of hydrogen peroxide in foods using a nanohybrid of nanoceria and carbon dots based on the target-promoted electron transfer | |
| Wang et al. | Synthesis of N-doped carbon dots and application in vanillin detection based on collisional quenching | |
| CN111117609B (en) | Fluorescent probe for quantitatively detecting riboflavin based on fluorescence resonance energy transfer ratio and preparation method and application thereof | |
| CN112964683A (en) | Preparation method and application of folic acid modified nitrogen-doped graphene quantum dot/silver nano fluorescent probe | |
| Liu et al. | A pH-responsive fluorometric and colorimetric system based on silicon quantum dots and 4-nitrophenol for urease activity detection | |
| Othman et al. | A newly synthesized boronic acid-functionalized sulfur-doped carbon dot chemosensor as a molecular probe for glucose sensing | |
| CN114106819B (en) | Preparation method of nano fluorescent probe and application of nano fluorescent probe in dual detection of nitrite ions and sulfite ions | |
| Lv et al. | A gold nanoparticle based colorimetric and fluorescent dual-channel probe for acetylcholinesterase detection and inhibitor screening | |
| CN114839171A (en) | Method for detecting tetracycline antibiotics in milk based on deep ultraviolet emission carbon spots | |
| CN104330393A (en) | Method for determining glucose by using gold nano-cluster as fluorescence probe | |
| Rastegarzadeh et al. | An optical redox chemical sensor for determination of iodide | |
| JP2001337087A (en) | Interference-eliminating membrane, test strip, kit and method for use in uric acid assay | |
| KR20140059608A (en) | Method for detecting target molecules using reflectance-based colorimetric analysis | |
| CN105115963A (en) | Heavy metal mercury detection method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |