CN109655519A - A kind of sex appraisal method of dioecian plant - Google Patents
A kind of sex appraisal method of dioecian plant Download PDFInfo
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- CN109655519A CN109655519A CN201910090379.2A CN201910090379A CN109655519A CN 109655519 A CN109655519 A CN 109655519A CN 201910090379 A CN201910090379 A CN 201910090379A CN 109655519 A CN109655519 A CN 109655519A
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 230000005518 electrochemistry Effects 0.000 claims abstract description 53
- 229910052799 carbon Inorganic materials 0.000 claims description 45
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 19
- 239000007853 buffer solution Substances 0.000 claims description 15
- 239000002270 dispersing agent Substances 0.000 claims description 13
- 229910021397 glassy carbon Inorganic materials 0.000 claims description 13
- 239000000872 buffer Substances 0.000 claims description 12
- 238000005070 sampling Methods 0.000 claims description 12
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 7
- 239000006185 dispersion Substances 0.000 claims description 6
- 239000008055 phosphate buffer solution Substances 0.000 claims description 4
- 210000001367 artery Anatomy 0.000 claims description 3
- 210000003462 vein Anatomy 0.000 claims description 3
- 238000004832 voltammetry Methods 0.000 claims description 3
- 238000011010 flushing procedure Methods 0.000 claims 1
- 239000004615 ingredient Substances 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 89
- 240000005717 Dioscorea alata Species 0.000 description 21
- 235000002723 Dioscorea alata Nutrition 0.000 description 21
- 235000002722 Dioscorea batatas Nutrition 0.000 description 21
- 235000008532 Dioscorea bulbifera Nutrition 0.000 description 21
- 235000006536 Dioscorea esculenta Nutrition 0.000 description 21
- 240000001811 Dioscorea oppositifolia Species 0.000 description 21
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 description 21
- 238000000083 pulse voltammetry Methods 0.000 description 21
- 241001674343 Taxus x media Species 0.000 description 18
- 238000001514 detection method Methods 0.000 description 18
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 244000050510 Cunninghamia lanceolata Species 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 239000007974 sodium acetate buffer Substances 0.000 description 3
- 239000000052 vinegar Substances 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 229960002319 barbital Drugs 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 235000011201 Ginkgo Nutrition 0.000 description 1
- 244000194101 Ginkgo biloba Species 0.000 description 1
- 235000008100 Ginkgo biloba Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000010159 dioecy Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
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- General Health & Medical Sciences (AREA)
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Abstract
The invention belongs to plant identification technical fields, more particularly to a kind of sex appraisal method of dioecian plant, include the following steps: that (1) detects the plant leaf blade sample of known gender, obtains the corresponding electrochemistry fingerprint standard spectrogram of plant leaf blade sample of different sexes；(2) plant leaf blade of gender to be identified is detected, obtains testing result；(3) testing result is compared with electrochemistry fingerprint standard spectrogram, obtains the gender of the plant of gender to be identified.The identification method of dioecian plant gender of the invention, the spectrogram shown according to the electrochemical behavior difference caused by different of ingredient in kindred plant male and female plant blade is different, to judge plant male and female plant；Identification method of the invention is simple and easy to do, low in cost, can be identified whenever and wherever possible, and accuracy is high, the gender of energy Rapid identification dioecian plant.
Description
Technical field
The invention belongs to plant identification technical fields, and in particular to a kind of sex appraisal method of dioecian plant.
Background technique
In economic plants, the phenomenon that dioecism, is very universal, as Taxus x media, air potato, Chinese yam and ginkgo etc.
It is all dioecious plant.The plant of different sexes often has different economic values, if being harvest with seed and fruit
Object, female plant are favored compared with by people；And sometimes with nutrition organs grow based on staminiferous plant have higher economic value.So female
In the cultivation of male different strain, reasonably selects male and female individual to increasing economic efficiency, it is all particularly significant to reduce the wasting of resources.
Initial people are to determine plant gender by rule of thumb by plant forms, however, most of dioecian plants are in property
Morphological differences is not obvious before Organ Differentiation and maturation, and influence of the formalness of plant vulnerable to external factor such as environment, mirror
The accuracy for determining result is not high.In recent years, plant early sex identification has on the different levels such as cell, biochemistry and molecule
Certain development, wherein DNA molecular marker technology substantially increases the standard of qualification result in the application in plant identification gender field
True property and reliability, but the technology, to the more demanding of laboratory and operator, appraisal cost is excessively high, is not suitable for pushing away on a large scale
Wide application.
Summary of the invention
Aiming at the above shortcomings existing in the prior art, the present invention provides a kind of sex identification side of dioecian plant
Method.
In order to achieve the above object of the invention, the invention adopts the following technical scheme:
A kind of sex appraisal method of dioecian plant, includes the following steps:
(1) the plant leaf blade sample of known gender is detected, the plant leaf blade sample for obtaining different sexes is corresponding
Electrochemistry fingerprint standard spectrogram；
(2) plant leaf blade of gender to be identified is detected, obtains testing result；
(3) testing result is compared with electrochemistry fingerprint standard spectrogram, obtains the plant of gender to be identified
Gender.
Preferably, the step (1) includes the following steps:
(10) the plant leaf blade sample that known gender is taken with capillary, is placed in glassy carbon electrode surface；
(11) take a certain amount of carbon nanotube-NMP dispersant liquid drop in by making on step (10) treated glass-carbon electrode
Plant leaf blade sample is fully wrapped around by drop；
(12) will by step (11) treated, glass-carbon electrode is respectively placed in two different buffers, to carry out arteries and veins
Rush voltammetry Scanning Detction；
(13) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded,
It is included in two-dimensional coordinate system to obtain the corresponding electrochemistry two dimension scatterplot fingerprint standard spectrogram of plant leaf blade sample of different sexes.
Preferably, the step (2) includes the following steps:
(20) plant leaf blade that gender to be identified is taken with capillary, is placed in glassy carbon electrode surface；
(21) take a certain amount of carbon nanotube-NMP dispersant liquid drop in by making on step (20) treated glass-carbon electrode
The plant leaf blade of gender to be identified is fully wrapped around by drop；
(22) will by step (21) treated, glass-carbon electrode is respectively placed in described two different buffers, with into
Row pulse voltammetry Scanning Detction；
(23) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded,
It is included in two-dimensional coordinate system to obtain the corresponding electrochemistry two dimension scatterplot fingerprint chromatogram of plant leaf blade of gender to be identified.
Preferably, the diameter of the plant leaf blade sample of the known gender is 1~2mm.
Preferably, the diameter phase of the plant leaf blade sample of the plant leaf blade and known gender of the gender to be identified
Together.
Preferably, the concentration of the carbon nanotube-NMP dispersion liquid is 1~2%.
Preferably, a certain amount of carbon nanotube-NMP dispersion liquid is 2~7 μ L.
Preferably, the buffer is phosphate buffer solution, citric acid-sodium citrate buffer, acetic acid-vinegar
Sour sodium buffer solution or calmine-hydrochloric acid buffer solution.
Preferably, the concentration of the buffer is 0.1~0.3mol/L.
Preferably, the condition of the pulse voltammetry Scanning Detction are as follows: sweep interval is -0.4~1.2V, scanning
Speed is 70~150mV/s, and the sampling interval is 1~5mV.
Compared with prior art, the present invention beneficial effect is:
The sex appraisal method of dioecian plant of the invention draws according to ingredient difference in kindred plant male and female plant blade
The spectrogram that the electrochemical behavior difference risen is shown is different, to judge plant male and female plant；Identification method of the invention is easy
It is easy, it is low in cost, it can be identified whenever and wherever possible, accuracy is high, the gender of energy Rapid identification dioecian plant；In addition,
It also has the advantage that
1, entire analytic process proposed by the present invention is not necessarily to expensive reagent and instrument, greatly reduces plant sex identification
Cost.
2, the present invention can carry out Sexual discriminating to plant in plant early stage, substantially increase the economic benefit of plantation.
3, plant early sex identification method proposed by the present invention is potential prepares mini kit, easily promotes, convenient on the spot
Detection.
Detailed description of the invention
Fig. 1 is the electrochemistry fingerprint chromatogram of the Taxus x media female plant of the embodiment of the present invention 1；
Fig. 2 is the electrochemistry fingerprint chromatogram of the Taxus x media staminiferous plant of the embodiment of the present invention 1；
Fig. 3 is the electrochemistry fingerprint chromatogram of the Taxus x media shoot to be measured of the embodiment of the present invention 1；
Fig. 4 is the electrochemistry fingerprint chromatogram of the air potato female plant of the embodiment of the present invention 2；
Fig. 5 is the electrochemistry fingerprint chromatogram of the air potato staminiferous plant of the embodiment of the present invention 2；
Fig. 6 is the electrochemistry fingerprint chromatogram of the air potato shoot to be measured of the embodiment of the present invention 2；
Fig. 7 is the electrochemistry fingerprint chromatogram of the Chinese yam female plant of the embodiment of the present invention 3；
Fig. 8 is the electrochemistry fingerprint chromatogram of the Chinese yam staminiferous plant of the embodiment of the present invention 3；
Fig. 9 is the electrochemistry fingerprint chromatogram of the Chinese yam shoot to be measured of the embodiment of the present invention 3.
Specific embodiment
Below by specific embodiment the technical scheme of the present invention will be further described explanation.
Embodiment 1:
The sex appraisal method of the Taxus x media of the present embodiment, comprising the following steps:
One, the acquisition of the electrochemistry fingerprint standard spectrogram of Taxus x media female plant
(1) Taxus x media female plant blade sample is taken with capillary, is placed in glassy carbon electrode surface；Wherein, Man Diya is red
The diameter of beans China fir female plant blade sample is 2mm；
(2) take the carbon nanotube-NMP dispersant liquid drop that 5 μ L mass concentrations are 1% above-mentioned by step (1) treated
On glass-carbon electrode, keep leaf sample fully wrapped around by drop；
It (3) will be slow by step (2) treated glass-carbon electrode is respectively placed in phosphate buffer and citric acid-sodium citrate
It rushes in solution, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection are as follows: scanning area
Between be -0.4~1.2V, scanning speed 80mV/s, sampling interval 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint chromatogram of Taxus x media female plant, as shown in Figure 1.
Two, the acquisition of the electrochemistry fingerprint standard spectrogram of Taxus x media staminiferous plant
(1) Taxus x media staminiferous plant blade sample is taken with capillary, is placed in glassy carbon electrode surface；Wherein, Man Diya is red
The diameter of beans China fir staminiferous plant blade sample is 2mm；
(2) take the carbon nanotube-NMP dispersant liquid drop that 5 μ L mass concentrations are 1% above-mentioned by step (1) treated
On glass-carbon electrode, keep leaf sample fully wrapped around by drop；
It (3) will be slow by step (2) treated glass-carbon electrode is respectively placed in phosphate buffer and citric acid-sodium citrate
It rushes in solution, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection are as follows: scanning area
Between be -0.4~1.2V, scanning speed 80mV/s, sampling interval 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint standard spectrogram of Taxus x media staminiferous plant, as shown in Figure 2.
Three, the acquisition of the electrochemistry fingerprint chromatogram of Taxus x media shoot to be measured
(1) Taxus x media shoot blade to be measured is taken with capillary, is placed in glassy carbon electrode surface；Wherein, Man Diya is red
The diameter of beans China fir shoot blade to be measured is 2mm；
(2) take carbon nanotube-NMP dispersant liquid drop that the concentration of 5 μ L is 1% by step (1) treated above-mentioned glass
On carbon electrode, keep leaf sample fully wrapped around by drop.
(3) phosphate buffer and citric acid-sodium citrate buffering will be respectively placed in by glass-carbon electrode after step (2) are handled
In solution, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection are as follows: sweep interval
For -0.4V~1.2V；Scanning speed is 80mV/s；Sampling interval is 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint chromatogram of Taxus x media shoot to be measured, as shown in Figure 3；
Four, the comparison of electrochemistry fingerprint chromatogram
By the electrochemistry two dimension scatterplot fingerprint chromatogram (Fig. 3) of Taxus x media shoot to be measured respectively with Taxus x media
The electrochemistry two dimension scatterplot fingerprint chromatogram (Fig. 1) of female plant, Taxus x media staminiferous plant electrochemistry two dimension scatterplot fingerprint standard spectrum
Figure (Fig. 2) compares, and finds the electrification of electrochemistry the two dimension scatterplot finger-print and Taxus x media female plant of shoot to be measured
It is high to learn two-dimentional scatterplot fingerprint standard spectrogram registration, therefore determines the shoot for female plant.
Embodiment 2:
The sex appraisal method of the air potato of the present embodiment, comprising the following steps:
One, the acquisition of the electrochemistry fingerprint standard spectrogram of air potato female plant
(1) air potato female plant blade sample is taken with capillary, is placed in glassy carbon electrode surface；Wherein, air potato female plant blade sample
Diameter be 2mm；
(2) take the carbon nanotube-NMP dispersant liquid drop that 5 μ L mass concentrations are 1% above-mentioned by step (1) treated
On glass-carbon electrode, keep leaf sample fully wrapped around by drop；
It (3) will treated, and glass-carbon electrode be respectively placed in citric acid-sodium citrate buffer solution and acetic acid-by step (2)
In sodium acetate buffer, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection
Are as follows: sweep interval is -0.4~1.2V, scanning speed 80mV/s, sampling interval 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint chromatogram of air potato female plant, as shown in Figure 4.
Two, the acquisition of the electrochemistry fingerprint standard spectrogram of air potato staminiferous plant
(1) air potato staminiferous plant blade sample is taken with capillary, is placed in glassy carbon electrode surface；Wherein, air potato staminiferous plant blade sample
Diameter be 2mm；
(2) take the carbon nanotube-NMP dispersant liquid drop that 5 μ L mass concentrations are 1% above-mentioned by step (1) treated
On glass-carbon electrode, keep leaf sample fully wrapped around by drop；
It (3) will treated, and glass-carbon electrode be respectively placed in citric acid-sodium citrate buffer solution and acetic acid-by step (2)
In sodium acetate buffer, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection
Are as follows: sweep interval is -0.4~1.2V, scanning speed 80mV/s, sampling interval 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint standard spectrogram of air potato staminiferous plant, as shown in Figure 5.
Three, the acquisition of the electrochemistry fingerprint chromatogram of air potato shoot to be measured
(1) air potato shoot blade to be measured is taken with capillary, is placed in glassy carbon electrode surface；Wherein, air potato shoot blade to be measured
Diameter be 2mm；
(2) take carbon nanotube-NMP dispersant liquid drop that the concentration of 5 μ L is 1% by step (1) treated above-mentioned glass
On carbon electrode, keep leaf sample fully wrapped around by drop.
(3) citric acid-sodium citrate buffer solution and acetic acid-vinegar will be respectively placed in by glass-carbon electrode after step (2) are handled
In sour sodium buffer solution, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection are as follows:
Sweep interval is -0.4V~1.2V；Scanning speed is 80mV/s；Sampling interval is 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint chromatogram of air potato shoot to be measured, as shown in Figure 6；
Four, the comparison of electrochemistry fingerprint chromatogram
The electrochemistry two dimension scatterplot fingerprint chromatogram (Fig. 6) of air potato shoot to be measured is two-dimentional with the electrochemistry of air potato female plant respectively
Scatterplot fingerprint chromatogram (Fig. 4), air potato staminiferous plant electrochemistry two dimension scatterplot fingerprint standard spectrogram (Fig. 5) compare, find it is to be measured
The electrochemistry two dimension scatterplot finger-print of shoot and the electrochemistry two dimension scatterplot fingerprint standard spectrogram registration of air potato staminiferous plant are high,
Therefore determine the shoot for staminiferous plant.
Embodiment 3:
The sex appraisal method of the Chinese yam of the present embodiment, comprising the following steps:
One, the acquisition of the electrochemistry fingerprint standard spectrogram of Chinese yam female plant
(1) Chinese yam female plant blade sample is taken with capillary, is placed in glassy carbon electrode surface；Wherein, Chinese yam female plant blade sample
Diameter be 2mm；
(2) take the carbon nanotube-NMP dispersant liquid drop that 5 μ L mass concentrations are 1% above-mentioned by step (1) treated
On glass-carbon electrode, keep leaf sample fully wrapped around by drop；
It (3) will treated, and glass-carbon electrode be respectively placed in NaAc_HAc buffer solution and barbital by step (2)
Receive-hydrochloric acid buffer solution in, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection
Are as follows: sweep interval is -0.4~1.2V, scanning speed 80mV/s, sampling interval 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint chromatogram of Chinese yam female plant, as shown in Figure 7.
Two, the acquisition of the electrochemistry fingerprint standard spectrogram of Chinese yam staminiferous plant
(1) Chinese yam staminiferous plant blade sample is taken with capillary, is placed in glassy carbon electrode surface；Wherein, Chinese yam staminiferous plant blade sample
Diameter be 2mm；
(2) take the carbon nanotube-NMP dispersant liquid drop that 5 μ L mass concentrations are 1% above-mentioned by step (1) treated
On glass-carbon electrode, keep leaf sample fully wrapped around by drop；
It (3) will treated, and glass-carbon electrode be respectively placed in NaAc_HAc buffer solution and barbital by step (2)
Receive-hydrochloric acid buffer solution in, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry is scanned the condition of detection
Are as follows: sweep interval is -0.4~1.2V, scanning speed 80mV/s, sampling interval 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint standard spectrogram of Chinese yam staminiferous plant, as shown in Figure 8.
Three, the acquisition of the electrochemistry fingerprint chromatogram of Chinese yam shoot to be measured
(1) Chinese yam shoot blade to be measured is taken with capillary, is placed in glassy carbon electrode surface；Wherein, Chinese yam shoot blade to be measured
Diameter be 2mm；
(2) take carbon nanotube-NMP dispersant liquid drop that the concentration of 5 μ L is 1% by step (1) treated above-mentioned glass
On carbon electrode, keep leaf sample fully wrapped around by drop.
(3) will after step (2) are handled glass-carbon electrode be respectively placed in concentration be 0.1mol/L Acetic acid-sodium acetate it is slow
It rushes in solution and calmine-hydrochloric acid buffer solution, detection is scanned with pulse voltammetry；Wherein, pulse voltammetry carries out
The condition of Scanning Detction are as follows: sweep interval is -0.4V~1.2V；Scanning speed is 80mV/s；Sampling interval is 1mV；
(4) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, column
Enter in two-dimensional coordinate system, obtains the electrochemistry two dimension scatterplot fingerprint chromatogram of Chinese yam shoot to be measured, as shown in Figure 9；
Four, the comparison of electrochemistry fingerprint chromatogram
The electrochemistry two dimension scatterplot fingerprint chromatogram (Fig. 9) of Chinese yam shoot to be measured is two-dimentional with the electrochemistry of Chinese yam female plant respectively
Scatterplot fingerprint chromatogram (Fig. 7), Chinese yam staminiferous plant electrochemistry two dimension scatterplot fingerprint standard spectrogram (Fig. 8) compare, find it is to be measured
The electrochemistry two dimension scatterplot finger-print of shoot and the electrochemistry two dimension scatterplot fingerprint standard spectrogram registration of Chinese yam female plant are high,
Therefore determine the shoot for female plant.
As seen from the above embodiment, the sex appraisal method of dioecian plant of the invention, according to kindred plant male and female
Plant male and female plant is judged as the spectrogram difference that the ingredients such as enzyme electrochemical behavior difference caused by different is shown in strain blade.This
The identification method that method provides is simple and easy to do, low in cost, can be identified whenever and wherever possible, and accuracy is higher, can Rapid identification
The gender of dioecian plant.
In above-described embodiment and its alternative, the mass concentration of carbon nanotube-NMP dispersion liquid can also for 0.8%,
1.1%, 1.2%, 1.3%, 1.5%, 1.6%, 1.8%, 1.9%, 2%, 2.2%.
In above-described embodiment and its alternative, the dripping quantity of carbon nanotube-NMP dispersion liquid can also be 2 μ L, 2.5 μ
L、3μL、3.5μL、4μL、4.5μL、5.5μL、6μL、6.5μL、7μL。
In above-described embodiment and its alternative, two different buffers can also be phosphate buffer solution and vinegar
Acid-sodium acetate buffer, phosphate buffer solution and calmine-hydrochloric acid buffer solution, citric acid-sodium citrate buffer
With calmine-hydrochloric acid buffer solution.
In above-described embodiment and its alternative, the concentration of above-mentioned buffer can also be 0.15mol/L,
0.18mol/L、0.2mol/L、0.25mol/L、0.28mol/L、0.3mol/L。
In above-described embodiment and its alternative, scanning speed in the condition of pulse voltammetry Scanning Detction can be with
For 70mV/s, 90mV/s, 100mV/s, 110mV/s, 120mV/s, 130mV/s, 140mV/s, 150mV/s；Sampling interval may be used also
Think 2mV, 3mV, 4mV, 5mV.
In above-described embodiment and its alternative, it is known that the diameter of the plant leaf blade sample of gender can also for 1mm,
1.2mm,1.5mm,1.6mm,1.8mm；The diameter phase of the plant leaf blade of gender to be identified and the plant leaf blade sample of known gender
Together.
In view of the numerous embodiments of the scheme of the present invention, each embodiment experimental data is huge numerous, is not suitable for arranging one by one herein
Explanation is lifted, but the content verified required for each embodiment and obtained final conclusion are close.So herein not to each reality
The verifying content for applying example is explained one by one.
The above is only that the preferred embodiment of the present invention and principle are described in detail, to the common skill of this field
For art personnel, the thought provided according to the present invention will change in specific embodiment, and these changes should also regard
For protection scope of the present invention.
Claims (10)
1. a kind of sex appraisal method of dioecian plant, which comprises the steps of:
(1) the plant leaf blade sample of known gender is detected, obtains the corresponding electrification of plant leaf blade sample of different sexes
Learn fingerprint standard spectrogram；
(2) plant leaf blade of gender to be identified is detected, obtains testing result；
(3) testing result is compared with electrochemistry fingerprint standard spectrogram, obtains the gender of the plant of gender to be identified.
2. a kind of sex appraisal method of dioecian plant according to claim 1, which is characterized in that the step
(1) include the following steps:
(10) the plant leaf blade sample that known gender is taken with capillary, is placed in glassy carbon electrode surface；
(11) take a certain amount of carbon nanotube-NMP dispersant liquid drop in by making plant on step (10) treated glass-carbon electrode
Blade sample is fully wrapped around by drop；
(12) will by step (11) treated, glass-carbon electrode is respectively placed in two different buffers, to carry out pulse volt
Peace method Scanning Detction；
(13) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, is included in
To obtain the corresponding electrochemistry two dimension scatterplot fingerprint standard spectrogram of plant leaf blade sample of different sexes in two-dimensional coordinate system.
3. a kind of sex appraisal method of dioecian plant according to claim 2, which is characterized in that the step
(2) include the following steps:
(20) plant leaf blade that gender to be identified is taken with capillary, is placed in glassy carbon electrode surface；
(21) take a certain amount of carbon nanotube-NMP dispersant liquid drop in by making wait reflect on step (20) treated glass-carbon electrode
Qualitative other plant leaf blade is fully wrapped around by drop；
(22) will by step (21) treated, glass-carbon electrode is respectively placed in described two different buffers, to carry out arteries and veins
Rush voltammetry Scanning Detction；
(23) electrochemical signals by two groups of acquisitions are standardized, and the current value of same current potential is corresponded, is included in
To obtain the corresponding electrochemistry two dimension scatterplot fingerprint chromatogram of plant leaf blade of gender to be identified in two-dimensional coordinate system.
4. a kind of sex appraisal method of dioecian plant according to claim 1-3, which is characterized in that institute
The diameter for stating the plant leaf blade sample of known gender is 1~2mm.
5. a kind of sex appraisal method of dioecian plant according to claim 4, which is characterized in that described to be identified
The plant leaf blade of gender is identical as the diameter of plant leaf blade sample of known gender.
6. a kind of sex appraisal method of dioecian plant according to claim 2 or 3, which is characterized in that the carbon
The concentration of nanotube-NMP dispersion liquid is 1~2%.
7. a kind of sex appraisal method of dioecian plant according to claim 2 or 3, which is characterized in that described one
Quantitative carbon nanotube-NMP dispersion liquid is 2~7 μ L.
8. a kind of sex appraisal method of dioecian plant according to claim 2 or 3, which is characterized in that described slow
Fliud flushing is phosphate buffer solution, citric acid-sodium citrate buffer, NaAc_HAc buffer solution or calmine-hydrochloric acid
Buffer solution.
9. a kind of sex appraisal method of dioecian plant according to claim 8, which is characterized in that the buffer
Concentration be 0.1~0.3mol/L.
10. a kind of sex appraisal method of dioecian plant according to claim 2 or 3, which is characterized in that the arteries and veins
Rush the condition of voltammetry Scanning Detction are as follows: sweep interval is -0.4~1.2V, and scanning speed is 70~150mV/s, sampling interval
For 1~5mV.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111413392A (en) * | 2020-03-25 | 2020-07-14 | 杭州电子科技大学 | Method for collecting electrochemical spectrum of lycoris seeds |
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| CN107201404A (en) * | 2017-06-15 | 2017-09-26 | 江西省农业科学院蔬菜花卉研究所 | A kind of molecular biology identification method and its application for Asparagus dioecian plant sex |
| CN108680621A (en) * | 2018-05-21 | 2018-10-19 | 江苏省中国科学院植物研究所 | A kind of floristic discrimination method |
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| CN107201404A (en) * | 2017-06-15 | 2017-09-26 | 江西省农业科学院蔬菜花卉研究所 | A kind of molecular biology identification method and its application for Asparagus dioecian plant sex |
| CN108680621A (en) * | 2018-05-21 | 2018-10-19 | 江苏省中国科学院植物研究所 | A kind of floristic discrimination method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111413392A (en) * | 2020-03-25 | 2020-07-14 | 杭州电子科技大学 | Method for collecting electrochemical spectrum of lycoris seeds |
| CN111413392B (en) * | 2020-03-25 | 2022-05-13 | 杭州电子科技大学 | Method for collecting electrochemical spectrum of lycoris seeds |
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