CN109627352A - A kind of decoloration process of corn silk polysaccharide - Google Patents
A kind of decoloration process of corn silk polysaccharide Download PDFInfo
- Publication number
- CN109627352A CN109627352A CN201811413024.4A CN201811413024A CN109627352A CN 109627352 A CN109627352 A CN 109627352A CN 201811413024 A CN201811413024 A CN 201811413024A CN 109627352 A CN109627352 A CN 109627352A
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- cotton
- corn silk
- decoloration
- deae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of corn silk polysaccharide discoloration methods, using percent of decolourization and polysaccharide retention rate as index, using 100mg corn silk polysaccharide as sample, obtaining optimum process condition is 100 ~ 110mL of elution volume, elution speed is 2.5 ~ 3.5mL/min, and cotton like DEAE dosage is 6.5 ~ 7.5g.The percent of decolourization of this technique reaches 85 ~ 95%, and polysaccharide retention rate reaches 90 ~ 95%.The present invention is efficient, convenient, provides a kind of effective new method for the purifying of polysaccharide.
Description
Technical field
The present invention relates to health food chemical fields, especially with cotton like DEAE cellulose to one kind of corn silk polysaccharide
Decoloration process.
Background technique
Ingredient much beneficial to human body is contained, comprising: volatile alkaloid, flavones, soap in corn stigma main product the Northeast
Glycosides, sterol, polysaccharide etc., wherein corn silk polysaccharide has anti-oxidant, antifatigue, enhances immunity of organisms, and tumour cell is inhibited to increase
Value and other effects.But when extracting Stigma Maydis crude polysaccharides, pigment is also easy to be extracted, deeper color is not only interfered more
The purity of sugar, the research in terms of having an effect on the qualitative, quantitative of polysaccharide, isolating and purifying and is active, so needing to decolourize to polysaccharide
Processing.In the extracting and developing purification process of corn silk polysaccharide, the decoloration of polysaccharide is an important link.Consulted related text
It is more to the report of polysaccharide decoloration both at home and abroad at present after offering, but due to polysaccharide origin difference, discoloration method is also not quite similar, has
Active carbon adsorption, Amberlyst process, hydrogen peroxide decoloring method.Peng Yaling etc. compares 6 kinds of resins to violet passion fruit pectin
The effect of polysaccharide decoloration, it is determined that polyamide decolorizing efficiency is best, optimum condition are as follows: sample volume 3.5BV, 3 BV/ of flow velocity
H, pH 3.5, percent of decolourization 52.78%, galacturonic acid retention rate are 85.62%, the results showed that polyamide sends out the decoloration to polysaccharide
Effect is preferable.But decolorization operations are complicated, processing is difficult, and polysaccharide loss rate is big.Zhou Hongli et al. has probed into jade using active carbon
The decoloration process of rice palpus polysaccharide, activated carbon dosage 2%, bleaching time 80min, heating temperature is 60 DEG C, and percent of decolourization is
14.76%, loss late is 13.01%, and active carbon adsorption has the characteristics that physicochemical property that is safe and nontoxic, will not destroying polysaccharide,
But percent of decolourization is low.Gong Chunyu et al. has probed into the decoloration process of corn silk polysaccharide absorption method, polysaccharide concentration 2 using D301R resin
Mg/mL, bleaching time 40m in, 7 g of resin content (weight in wet base, 0.35 g/m L), 40 DEG C of bleaching temperature, practical percent of decolourization are
79.6%, the results showed that after macroreticular resin has good decolorizing effect, but polysaccharide concentration increases, decolorizing effect will become very
It is undesirable.Then the technique that Gong Chunyu et al. decolourizes to corn silk polysaccharide using response surface optimization hydrogen peroxide, hydrogen peroxide
Additive amount 0.15: 1(V: V), pH 12, decolourize 3 h, and 40 DEG C of bleaching temperature.Using this technique, in 10 mg/mL of polysaccharide concentration
When, the practical percent of decolourization of corn silk polysaccharide is up to 69.3%, polysaccharide retention rate 60.1%.Hydrogen peroxide has good corn as the result is shown
Palpus decolorization, but hydroperoxidation condition is violent, can destroy polysaccharide, and in decolorization, since hydrogen peroxide can produce
When giving birth to a large amount of bubbles, therefore using this method, the requirement to equipment and operation is higher than adsorption decoloring method.Zhao Wenzhu et al. uses anti-glue
Beam solution decoloring method removes the colors in corn silk polysaccharide, and percent of decolourization reaches 91.43%, and polysaccharide recovery reaches
77.59%, the monosaccharide group for determining corn silk polysaccharide after purification becomes mannose, galactolipin, xylose, arabinose and fructose five
Kind monosaccharide.Sun Xiaoxue et al. has probed into the technique of DEAE cellulose purification corn stigma total starches, and best purifying process is applied sample amount
0.75BV can be more fully hereinafter by neutral total starches and acidity respectively with the elution of the 0.1mol/L NaCl solution of 10BV water and 15BV
Total starches elute；Neutral polysaccharide and acidic polysaccharose account for the 30.43% and 58.88% of total starches respectively, and purity is respectively increased
About 2.5 and 2 times, this method effect purification effect is preferable, but DEAE-52 is expensive, is not suitable for the high-volume of corn silk polysaccharide
Purifying.And polysaccharide often exists in the form of acid heteroglycan uronic acid, uronic acid itself or contains uronic acid structural unit
Oligosaccharide and polysaccharide show highly important bioactivity, so should improve uronic acid as far as possible during polysaccharide purification
Retention rate.To solve this problem, this technique decolourizes to corn silk polysaccharide using cotton like DEAE cellulose, response surface analysis
Method optimization.Cotton like DEAE cellulose is anion exchange resin, is more advantageous to the reservation of uronic acid, and less expensive, renewable,
Purification effect is relatively good, is suitble to the purifying of polysaccharide.
Summary of the invention
The present invention provides a kind of cotton like DEAE cellulose to the decoloration process of Stigma Maydis crude polysaccharides.
It is an object of the invention to: a kind of discoloration method using cotton like DEAE cellulose to Stigma Maydis crude polysaccharides, the party are provided
Method is convenient, pollution-free, cheap, reusable, and decolorizing effect is good, and polysaccharide retention rate is high, and it is slightly more to improve corn stigma
The purification efficiency of sugar, can be used for industrialized production.
It is decolourized herein using cotton like DEAE cellulose to corn silk polysaccharide, and is response with percent of decolourization and polysaccharide retention rate
Value, using the optimal parameter of Responds Surface Methodology optimization decoloration process.For corn silk polysaccharide purifying provide it is efficient and convenient
Purification process.
A kind of technique as described in claim 1 decolourized using cotton like DEAE cellulose to corn silk polysaccharide, it is characterised in that press
Following steps carry out:
(1) preparation of Stigma Maydis crude polysaccharides: taking dry corn stigma 30g according to 90 DEG C of extraction 1h of 1:30 liquid-to-solid ratio, be repeated twice, and closes
And filtrate, it is concentrated into the 1/10 of filtrate volume, 95% ethyl alcohol is added to static 12h at 80%, 4 DEG C, centrifugation, sediment fraction acetone
Washing, freeze-drying, obtains Thick many candies powder, and polyoses content is 18.82%(g/g in Stigma Maydis crude polysaccharides).
(2) pretreatment of cotton like DEAE cellulose: weighing a certain amount of DEAE cellulose, for 24 hours with sufficient distilled water immersion, so
3h is impregnated with the NaOH of 0.5mol/L afterwards, is filtered, is washed to neutrality with distillation, then with 0.5mol/L HCl immersion 3h, is washed till
Property, it drains spare.
(3) the single factor test optimal conditions that single factor experiment finds out decoloration, such as effluent volume, elution speed, cotton like are first passed through
DEAE cellulose dosage.
(4) on the basis of experiment of single factor, using solid-liquid ratio, extraction time, pH value as influence factor, 3 levels are respectively taken, are used
The response surface analysis experiment of center combination design Box-Behnken design Three factors-levels optimizes decoloration process, obtains
Optimum process, i.e. effluent volume are 100 ~ 110mL, and elution speed is 2.5 ~ 3.5mL/min, and cotton like DEAE cellulose dosage is
6.5~7.5g。
(5) optimum process that response surface obtains is verified, this technique percent of decolourization reaches 85% ~ 91%, and polysaccharide retention rate reaches
90% ~ 93.61%, purity of polysaccharide reaches 70% ~ 76%.
Detailed description of the invention
Fig. 1 is influence of the effluent volume to corn silk polysaccharide decolorizing effect；Fig. 2 is that elution speed is de- to corn silk polysaccharide
The influence of color effect；Fig. 3 is the influence that DEAE dosage decolourizes to corn silk polysaccharide.
Specific embodiment
Specific introduce is made to the present invention below in conjunction with chart and specific implementation operation.
The preparation of one Stigma Maydis crude polysaccharides of embodiment
It takes dry corn stigma 30g according to 90 DEG C of extraction 1h of 1:30 liquid-to-solid ratio, is repeated twice, merging filtrate is concentrated into filtrate volume
1/10,95% ethyl alcohol is added to static 12h at 80%, 4 DEG C, centrifugation, sediment fraction acetone washing, freeze-drying obtains slightly more
Icing Sugar end 2.232g, purity of polysaccharide 18.82%.
The measurement and calculating of two percent of decolourization of embodiment and polysaccharide retention rate
It is decolourized after purification to corn stigma, the absorbance of polysaccharide solution, calculates percent of decolourization before and after measurement decoloration at 420nm.
Using polyoses content of the phend-sulphuric acid before and after measurement decoloration at 490nm, polysaccharide retention rate is calculated.Using an xenol
Method measures the content of uronic acid in polysaccharide before and after decoloration at 528nm, calculates uronic acid retention rate.
Percent of decolourization (%)=(ABefore decoloration-AAfter decoloration)/AAfter decoloration×100%
Polysaccharide retention rate (%)=MPolysaccharide after decoloration/MPolysaccharide before decolourizing×100%
Uronic acid retention rate (%)=MUronic acid after decoloration/MUronic acid before decolourizing×100%
Three AB-8 macroreticular resin of embodiment decolourizes to corn silk polysaccharide
The pretreatment of AB-8 macroreticular resin: being impregnated for 24 hours with 95% ethyl alcohol, and wet method dress post after making it sufficiently be swollen and tentatively clean is used
The flowing cleaning of 95% ethyl alcohol, checks frequently, until efflux mixes (1:5) with water without white opacity, is then washed to distillation
Without alcohol taste.
Resin volume is 50mL, prepares the corn silk polysaccharide solution of 2mg/mL, and loading volume is that 50mL flow velocity is 2 ~ 3 BV/h.
AB-8 macroreticular resin decolourizes to corn silk polysaccharide, and percent of decolourization is 76.92% ± 2.34%, and polysaccharide retention rate is
52.2% ± 1.89%, uronic acid retention rate is 41.89% ± 1.45%
The processing of four cotton like DEAE cellulose of embodiment
A certain amount of DEAE cellulose is weighed, for 24 hours with sufficient distilled water immersion, then impregnates 3h with the NaOH of 0.5mol/L,
It filters, is washed to neutrality with distillation, then impregnate 3h with 0.5mol/L HCl, is washed till neutrality, drains spare.
Embodiment five determines the best single factor test condition of decoloration process
In order to probe into the best single factor test condition of corn silk polysaccharide polychrome technique, effluent volume, elution speed, cotton like are chosen
Three factors of DEAE cellulose dosage find out single factor test using polysaccharide percent of decolourization and polysaccharide retention rate as response for influence factor
Optimum level.
(1) influence of the effluent volume to corn silk polysaccharide decolorizing effect
Above-mentioned Thick many candies 100mg is taken, 10mL distilled water is added, upper cotton like DEAE cellulose chromatographic column, control after all dissolutions
Elution speed is 2mL/min, and cotton like DEAE mass is 7g, investigate effluent volume (60mL, 80mL, 100mL, 120mL,
140mL) to the influence of polysaccharide purification.
By Figure of description Fig. 1 it is found that polysaccharide percent of decolourization reaches maximum value, polysaccharide retention rate when effluent volume is 100mL
It is all gradually increased, but increasing degree is maximum when 100mL, as volume increases retention rate not too big effect, with
Effluent volume increases, and polysaccharide retention rate is gradually reduced, so decolorizing effect is best when effluent volume selection 100mL, polysaccharide
Retention rate is also higher.
(2) influence of the elution speed to corn silk polysaccharide decolorizing effect
Above-mentioned Thick many candies 100mg is taken, 10mL distilled water is added, upper cotton like DEAE cellulose chromatographic column, control after all dissolutions
Effluent volume is 100mL, and cotton like DEAE mass is 7g, investigate flow velocity (1mL/min, 2mL/min, 3mL/min, 4mL/min,
5mL/min) to the influence of purity of polysaccharide.
It is found that as elution speed improves, polysaccharide retention rate and percent of decolourization change significantly Figure of description Fig. 2, and big
Body trend be it is identical, elution speed be 3mL/min when, percent of decolourization and polysaccharide retention rate reach maximum value, thus selection
3mL/min is optimum flow rate.
(3) influence that DEAE dosage decolourizes to corn silk polysaccharide
Above-mentioned Thick many candies 100mg is taken, 10mL distilled water is added, upper cotton like DEAE cellulose chromatographic column, control after all dissolutions
Elution speed is 2mL/min, effluent volume 100mL, investigates the dosage (3g, 5g, 7g, 9g, 11g, 13g) of cotton like DEAE to more
The influence of sugared purification effect.
By Figure of description Fig. 3 it is found that with DEAE mass increase, percent of decolourization is improving always, and polysaccharide retention rate exists
It is reduced again after polysaccharide retention rate increases when DEAE mass is 7g, in order to reduce polysaccharide loss, and can have preferable decoloration
Effect selects 7g for best DEAE mass.
By experiment of single factor result it is found that the best single factor test condition of corn silk polysaccharide decoloration process is；Effluent volume
100mL, elution speed 3mL/min, cotton like DEAE dosage are 7g.
Six Box-Benhnken central combination design of embodiment probes into decoloration optimum process
On the basis of experiment of single factor, using center combination design Box-Behnken design scheme, chooses elution speed, washes
Three de- liquid product, cotton like DEAE mass factors design Three factors-levels using corn silk polysaccharide solution percent of decolourization as response
Response surface analysis experiment, three horizontal uses -1,0,1 of each factor are encoded, such as table 1.
1 response surface empirical factor water-glass of table
Response surface experimental design and result by the experimental design function of Design-Expert 8.0.6 statistical analysis software it is found that
Using the percent of decolourization of corn silk polysaccharide as response, with elution speed (A), effluent volume (B), DEAE mass (C) for variable, build
The center combination design of vertical Three factors-levels includes 17 groups of experimental programs, and each experimental program carries out three groups of measurements and is averaged
Value.Specific experiment design such as table 2.
2 Box-Behnken experimental design of table and result
The analysis of 3 Box-Behnken test statistics of table
Note: P>0.05 is not significant, and P<0.0001 is extremely significant
The foundation of model and conspicuousness and inspection
It is analyzed using Design-Expert 8.0.6 software and is fitted recurrence by data in regression analysis Table 2, washed
Three de- speed (A), effluent volume (B), DEAE dosage (C) factors are to the quadratic regression equation percent of decolourization of the percent of decolourization of polysaccharide
=90.82-1.48A-0.70B-0.11C-1.46AB-1.93AC+1.4BC+0.059A2-1.06B2-6.06C2.In order to examine jade
The validity of rice palpus polysaccharide purification technique regression equation, carries out significance test to above-mentioned equation, as a result such as table 3, is divided by variance
Analysing regression equation can be with virtualphase is not significant (P>0.05), and model group is extremely significant (P<0.0001), the R of percent of decolourization2=
0.9866, R(Adj) 2=0.9694, illustrate that the models fitting is good, model is set up.Influence polysaccharide decolorizing effect secondary factors be
Cotton like DEAE cellulose dosage > effluent volume > elution speed.
By the analysis of regression model, it is 100 ~ 110mL, elution speed that the optimum process that can determine decoloration, which is effluent volume,
For 2.5 ~ 3.5mL/min, cotton like DEAE cellulose dosage is 6.5 ~ 7.5g.By verification test, the percent of decolourization of this technique reaches
85% ~ 91%, polysaccharide retention rate reaches 90% ~ 93.61%, and uronic acid retention rate reaches 75% ~ 84%.With the decoloration of AB-8 macroreticular resin
Effect is compared, and the percent of decolourization and polysaccharide retention rate of cotton like DEAE cellulose are relatively high.After the decoloration of cotton like DEAE cellulose,
Purity of polysaccharide reaches 70% ~ 76%.
Embodiment seven after purification in corn silk polysaccharide monosaccharide component and its molecular weight measurement
1, the measurement of monosaccharide composition
Corn silk polysaccharide sample after purification is accurately weighed, is placed in reaction kettle, the trifluoroacetic acid (TFA) of 3mL 2mol/L is added
Solution, sealing, which is put into 110 DEG C of baking ovens, hydrolyzes 5h, and supernatant is taken to be evaporated, and is washed repeatedly 3 times with methanol, is evaporated hydrolysate.
Above-mentioned hydrolysate is taken, is dissolved in water and is settled to 10mL, accurately pipettes in 0.2mL and glass centrifuge tube, is separately added into
The methanol solution of the 1- phenyl -3- methyl -5- pyrazoles beautiful jade ketone (PMP) of 0.2mL 0.5mol/L and the hydrogen-oxygen of 0.2mL 0.3mol/L
Change sodium solution in 70 DEG C of heating water bath 60min, the hydrochloric acid solution that 0.2mL 0.3mol/L is added after cooling neutralizes, and adds 1mL
Chloroform extraction, centrifugation are removed supernatant, are repeated 3 times, and are diluted with water that be settled to 5mL spare.
HPLC condition: splitter: ODS2 C18Column (250 × 4.6mm2, 5 μm), mobile phase: acetonitrile -0.05mol/mL phosphate is slow
Rush pH=6.8(18:82), flow velocity: 0.8mL/min, column temperature: 30 DEG C, UV detector, Detection wavelength 245nm, sample volume is
20μL.Mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactolipin, xylose, arabinose, rock
Algae sugar is as standard items.
Measuring monosaccharide in corn silk polysaccharide after purification and its molar ratio is Man:Rib:Rha:GluA:GalA:Glu:
Gal: Xyl: Arab: Fuc=27.9: 1: 10.2: 7.3: 13.2: 41.6: 51.4: 8.6: 14.7: 3.7。
Compared with other discoloration methods, the composition of monosaccharide contained by the corn silk polysaccharide after cotton like DEAE cellulose purification is more rich
Richness, the monosaccharide containing the active effect such as uronic acid and galactolipin.
2. the measurement of corn silk polysaccharide molecular weight
The corn silk polysaccharide solution of 2mg/mL is prepared, it is spare with 0.45 μm of membrane filtration.Data processing uses N2000 GPC color
Spectrum work station is recorded.Use the glucan of the different molecular weights size such as T-40, T-70, T-100, T-400 as standard respectively
Product establish calibration curve
HPLC condition: splitter: KS-804 sephadex column (8.0 × 300mm2), mobile phase: ultrapure water, flow velocity: 0.8
ML/min, column temperature: 50 DEG C, detector temperature: 35 DEG C, differential refraction detector.
Corn silk polysaccharide molecular weight ranges are 30 ~ 1230 kDa after purification.
According to the experimental results, it is decolourized using cotton like DEAE to polysaccharide, technological operation is convenient, and percent of decolourization and polysaccharide retain
Rate is high, greatly increases the purification efficiency of polysaccharide, for polysaccharide isolate and purify provide it is a kind of new efficient and convenient pure
Change method.
Claims (5)
1. the optimization based on corn silk polysaccharide decoloration process, it is characterised in that carried out with cotton like DEAE cellulose to corn silk polysaccharide
Decoloration, comprising the following steps:
(1) experiment of single factor, using 100mg corn silk polysaccharide as sample, to its effluent volume, elution speed, cotton like DEAE is fine
It ties up plain dosage to be probed into, obtain best single factor test condition: effluent volume is 100 ± 2mL, and elution speed is 3 ± 0.26mL/
Min, cotton like DEAE cellulose dosage are 7 ± 0.17g；
(2) on the basis of experiment of single factor, using the sound of center combination design Box-Behnken design Three factors-levels
It answers surface analysis to test, show that decoloration optimum process, i.e. 100 ~ 110mL of effluent volume, elution speed are 2.5 ~ 3.5mL/min,
Cotton like DEAE dosage is 6.5 ~ 7.5g.
2. application as described in claim 1, which is characterized in that this polysaccharide decoloration process is using cotton like DEAE cellulose.
3. application as described in claim 1, which is characterized in that in step (2), resulting best decoloration process condition is washed
De- liquid product is 100 ~ 110mL, and elution speed is 2.5 ~ 3.5mL/min, and cotton like DEAE cellulose dosage is 6.5 ~ 7.5g.
4. application as described in claim 1, which is characterized in that monosaccharide and its molar ratio are in corn silk polysaccharide after purification
Man: Rib: Rha: GluA: GalA: Glu: Gal: Xyl: Arab: Fuc=27.9: 1: 10.2: 7.3: 13.2:
41.6:51.4:8.6:14.7:3.7 wherein the monosaccharide containing the active effect such as uronic acid and galactolipin.
5. application as described in claim 1, which is characterized in that corn silk polysaccharide molecular weight ranges after purification are 30 ~ 1230
kDa。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811413024.4A CN109627352B (en) | 2018-11-26 | 2018-11-26 | Decolouring process of corn stigma polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811413024.4A CN109627352B (en) | 2018-11-26 | 2018-11-26 | Decolouring process of corn stigma polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109627352A true CN109627352A (en) | 2019-04-16 |
| CN109627352B CN109627352B (en) | 2021-06-11 |
Family
ID=66068905
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811413024.4A Active CN109627352B (en) | 2018-11-26 | 2018-11-26 | Decolouring process of corn stigma polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109627352B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296712A (en) * | 2020-03-18 | 2020-06-19 | 吉林化工学院 | Solid beverage of sea buckthorn leaf water extract and preparation method thereof |
| CN112409502A (en) * | 2020-07-01 | 2021-02-26 | 吉林化工学院 | Method for separating and characterizing ginseng polysaccharide with immunoregulation and anti-tumor activity |
| CN113616743A (en) * | 2021-07-24 | 2021-11-09 | 吉林化工学院 | Optimized corn stigma water extraction process based on blood glucose reducing activity |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709093A (en) * | 2009-12-17 | 2010-05-19 | 广西大学 | Method for preparing blumea riparia water-soluble polysaccharides |
| CN101748173A (en) * | 2009-12-03 | 2010-06-23 | 渤海大学 | Corn silk polysaccharide separation and decoloring method |
| CN101914166A (en) * | 2010-08-25 | 2010-12-15 | 吉林化工学院 | Technical method for decoloring and deproteinizing corn stigma polysaccharide |
| CN102051809A (en) * | 2009-11-09 | 2011-05-11 | 西安交大保赛生物技术股份有限公司 | Preparation of modified fibers and fiber fabrics |
| CN102608247A (en) * | 2012-01-12 | 2012-07-25 | 吉林化工学院 | Method for identifying stigma maydis polysaccharide by utilizing thin layer chromatography |
| CN104945533A (en) * | 2015-07-28 | 2015-09-30 | 齐齐哈尔大学 | Preparation method of pure active corn stigma polysaccharide |
| CN107245113A (en) * | 2017-07-10 | 2017-10-13 | 吉林化工学院 | Corn silk polysaccharide extract with antitumaous effect and preparation method thereof |
-
2018
- 2018-11-26 CN CN201811413024.4A patent/CN109627352B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102051809A (en) * | 2009-11-09 | 2011-05-11 | 西安交大保赛生物技术股份有限公司 | Preparation of modified fibers and fiber fabrics |
| CN101748173A (en) * | 2009-12-03 | 2010-06-23 | 渤海大学 | Corn silk polysaccharide separation and decoloring method |
| CN101709093A (en) * | 2009-12-17 | 2010-05-19 | 广西大学 | Method for preparing blumea riparia water-soluble polysaccharides |
| CN101914166A (en) * | 2010-08-25 | 2010-12-15 | 吉林化工学院 | Technical method for decoloring and deproteinizing corn stigma polysaccharide |
| CN102608247A (en) * | 2012-01-12 | 2012-07-25 | 吉林化工学院 | Method for identifying stigma maydis polysaccharide by utilizing thin layer chromatography |
| CN104945533A (en) * | 2015-07-28 | 2015-09-30 | 齐齐哈尔大学 | Preparation method of pure active corn stigma polysaccharide |
| CN107245113A (en) * | 2017-07-10 | 2017-10-13 | 吉林化工学院 | Corn silk polysaccharide extract with antitumaous effect and preparation method thereof |
Non-Patent Citations (8)
| Title |
|---|
| 姜涌明等编著: "《蛋白质化学》", 31 December 1997, 扬州大学农学院 * |
| 孙晓雪等: ""DEAE 纤维素纯化玉米须总多糖的工艺优选"", 《中国实验方剂学杂志》 * |
| 宫春宇等: ""响应面法优化玉米须多糖氧化脱色工艺"", 《粮食与油脂》 * |
| 宫春宇等: ""玉米须多糖吸附法脱色工艺条件研究"", 《食品科技》 * |
| 李亚平: ""玉米须多糖结构表征及其生物活性的研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
| 董文霞等: ""DEAE-52 纤维素静态法分离纯化桦褐孔菌多糖的工艺优化"", 《食品工业科技》 * |
| 陈祥洪: ""湖北麦冬抗II型糖尿病活性与物质基础研究"", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
| 黄森: ""霍山石斛多糖提取分离以及抗肿瘤活性的研究"", 《中国学位论文全文数据库》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296712A (en) * | 2020-03-18 | 2020-06-19 | 吉林化工学院 | Solid beverage of sea buckthorn leaf water extract and preparation method thereof |
| CN112409502A (en) * | 2020-07-01 | 2021-02-26 | 吉林化工学院 | Method for separating and characterizing ginseng polysaccharide with immunoregulation and anti-tumor activity |
| CN114524887A (en) * | 2020-07-01 | 2022-05-24 | 吉林化工学院 | Method for separating and characterizing ginseng polysaccharide with pharmaceutical activity function |
| CN113616743A (en) * | 2021-07-24 | 2021-11-09 | 吉林化工学院 | Optimized corn stigma water extraction process based on blood glucose reducing activity |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109627352B (en) | 2021-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chen et al. | Comparison of different extraction methods for polysaccharides from bamboo shoots (Chimonobambusa quadrangularis) processing by-products | |
| Hua et al. | Structural characterization and DPPH· radical scavenging activity of a polysaccharide from Guara fruits | |
| CN109627352A (en) | A kind of decoloration process of corn silk polysaccharide | |
| Liu et al. | Cellulase-assisted extraction, characterization, and bioactivity of polysaccharides from Polygonatum odoratum | |
| CN108727509B (en) | Moso bamboo shoot shell arabinogalactan and preparation and application thereof | |
| Dufrechou et al. | Protein/polysaccharide interactions and their impact on haze formation in white wines | |
| CN104498564A (en) | Low molecular weight chondroitin sulfate preparation method | |
| CN106800586A (en) | A kind of method of Moringa protein high efficiency extraction | |
| Yang et al. | Structure, physicochemical characterisation and properties of pectic polysaccharide from Premma puberula pamp. | |
| Tu et al. | A novel polysaccharide from Hericium erinaceus: Preparation, structural characteristics, thermal stabilities, and antioxidant activities in vitro | |
| CN111793141B (en) | Pleurotus citrinopileatus mycelium polysaccharide and preparation method and application thereof | |
| Fuso et al. | DPPH radical scavenging activity of xylo-oligosaccharides mixtures of controlled composition: A step forward in understanding structure–activity relationship | |
| Liu et al. | Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice | |
| CN112210576A (en) | High-purity kestose and preparation method thereof | |
| Tang et al. | Ultrasonic-assisted extraction, analysis and properties of purple mangosteen scarfskin polysaccharide and its acetylated derivative | |
| KR101628769B1 (en) | Preparation method of sugar mixture composition containing fructooligosaccharides | |
| Yang et al. | Optimization of the extraction process and antioxidant activity of Polysaccharide extracted from Centipeda minima | |
| CN110003354A (en) | A kind of flash preparation process of Fructus Hippophae polysaccharide with anti-oxidation function | |
| Fan et al. | Structure, physicochemical properties, antioxidant, and hypoglycemic activities of water‐soluble polysaccharides from millet bran | |
| CN104672196A (en) | Method for enhancing oxidation resistance of blueberry anthocyanidin | |
| Thi Thanh et al. | Structural characterization of agar extracted from six red seaweed species growing in the coast of Vietnam | |
| CN114874346A (en) | Lepista nuda polysaccharide and preparation method and application thereof | |
| Chen et al. | Structural characterization, molecular dynamic simulation, and conformational visualization of a water-soluble glucan with high molecular weight from Gastrodia elata Blume | |
| Li et al. | Structural characterization of polysaccharides in waste liquor produced by wet decortication of sesame seeds | |
| CN105399870B (en) | A kind of low-anticoagulant heparin, its oligosaccharide and preparation method thereof and the application in the medicine for preparing anti-alzheimer's disease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |