CN109621007A - A kind of preparation method of the surface coating of bone grafting material - Google Patents

A kind of preparation method of the surface coating of bone grafting material Download PDF

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Publication number
CN109621007A
CN109621007A CN201811636068.3A CN201811636068A CN109621007A CN 109621007 A CN109621007 A CN 109621007A CN 201811636068 A CN201811636068 A CN 201811636068A CN 109621007 A CN109621007 A CN 109621007A
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CN
China
Prior art keywords
preparation
surface coating
bone grafting
grafting material
hydroxyapatite
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811636068.3A
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Chinese (zh)
Inventor
周青宇
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Sichuan Chronoswiss Biological Polytron Technologies Inc
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Sichuan Chronoswiss Biological Polytron Technologies Inc
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Application filed by Sichuan Chronoswiss Biological Polytron Technologies Inc filed Critical Sichuan Chronoswiss Biological Polytron Technologies Inc
Priority to CN201811636068.3A priority Critical patent/CN109621007A/en
Publication of CN109621007A publication Critical patent/CN109621007A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/34—Macromolecular materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/28—Materials for coating prostheses
    • A61L27/30—Inorganic materials
    • A61L27/32—Phosphorus-containing materials, e.g. apatite
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2389/00—Characterised by the use of proteins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2489/00—Characterised by the use of proteins; Derivatives thereof

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Dermatology (AREA)
  • General Health & Medical Sciences (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The invention discloses a kind of preparation methods of the surface coating of bone grafting material, it is related to biomedical materials field, the following steps are included: gelatin, fibroin albumen, hydroxyapatite are sufficiently mixed by good solvent, crosslinking agent is added, reaction obtains finished product at a certain temperature.The present invention is stronger by the composite coating hydrophily that the mixture progress grafting modification to gelatin and hydroxyapatite is prepared, further improve contact angle and tensile strength, the interface binding power between coating and matrix is increased, it is made to be firmly fixed at material surface.

Description

A kind of preparation method of the surface coating of bone grafting material
Technical field
The present invention relates to biomedical materials field, especially a kind of preparation method of the surface coating of bone grafting material.
Background technique
Natural bone tissue mainly includes with hydroxyapatite inorganic phase as main component and the organic phase based on collagen. And nanometer Hap(hydroxyapatite) closely similar with the inorganic constituents of nature bone, there is excellent bioactivity and osteoconductive, It is thus considered as the ideal material of bone tissue engineer.But its mechanical property is poor when pure inorganic HAp is used as biomaterial, It cannot be used for the stress support at load-bearing bone, while HAp only has osteoconductive, be unfavorable for promoting after it is implanted into bone defect position Into the depth and quantity of new Bone Ingrowth, therefore, simple HAp material is difficult to repair the bone defect of pathological state or large volume, i.e., Osteoinductive is poor.
Gelatin is the collagenous portion hydrolysate and a kind of protein of skin, bone, cartilage and ligament, have with The identical amino acid of collagen shows good biocompatibility and weaker antigenicity, while also having preferable cell viscous Attached effect and osteoinductive, but its absorption is too fast and mechanical performance is poor.The two is carried out compound, that is, synthesizes compound painting Material, can be improved biocompatibility, engineering properties and osteoinductive.Therefore, composite coating is carried out to bone holder material surface Processing, so as to improve the compatibility of bone holder material.It is compound using various composition, construct the bio-medical material with excellent performance Material, the gelatin and hydroxyapatite closely similar with natural bone tissue are introduced into material surface simultaneously, make its have both raw material and The advantage of coating material.
In the prior art, Chinese patent CN 104404480 discloses a kind of Mg alloy surface and prepares hydroxyapatite and bone Collagen meets the method for coating, the preparation including deposition solution, and the concentration of calcium nitrate is 0.02- in deposition solution 0.05mol/L, the concentration of sodium phosphate are 0.002-0.005mol/L, and the concentration of bone collagen is 0.5-2.0g/L, are deposited molten The pH value of liquid is 3.0-4.0, and magnesium alloy is cleaned by ultrasonic 8-10min after grinding process in alcohol and acetone, then will Magnesium alloy, which is placed in deposition liquid, deposits 6-24h, and the deposition initial stage uses power for 100-150W, and frequency is the super of 30 ~ 45KHz 5-15min is swung in acoustic shock.Preparation method of the present invention is simple, the degradation of preparation-obtained hydroxyapatite/bone collagen coating Speed is low, and the biological medical magnesium alloy with the coating has preferable corrosion resistance, and ossein egg is added in deposition solution It is white, the forming core of hydroxyapatite can be accelerated and grown up, the corrosion resistance of material is improved.
The composite coating being prepared is simply mixed in hydroxyapatite and bone collagen in the patent, gelatin is reunited Or conformational isomerism causes coating surface uneven, has apparent phenomenon of phase separation between HAp and gelatin continuous phase, makes coating More easily degrade.
Summary of the invention
Goal of the invention of the invention is: in view of the above problems, providing a kind of surface coating of bone grafting material Preparation method, increase the interface binding power between coating and matrix, it made to be firmly fixed at material surface.
The technical solution adopted by the invention is as follows:
A kind of preparation method of the surface coating of bone grafting material, comprising the following steps: by gelatin, fibroin albumen, hydroxy-apatite Stone is sufficiently mixed by good solvent, and crosslinking agent is added, and reaction obtains finished product at a certain temperature.
Further, it include gelatin 2 ~ 3%, fibroin albumen 1 ~ 1.5%, hydroxyapatite 5 ~ 8% according to mass percent.
Further, the good solvent includes one of tetrahydrofuran, ether, acetaldehyde, formaldehyde, toluene.
Further, crosslinking agent carbodiimides.
Further, the reaction temperature is 45 ~ 70 DEG C.
Further, crosslinking agent chitosan.
Further, the reaction temperature is 60 ~ 70 DEG C.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
1, the present invention carries out the composite coating that grafting modification is prepared by the mixture to gelatin and hydroxyapatite Hydrophily is stronger, further improves contact angle and tensile strength, increases the interface binding power between coating and matrix, makes It is firmly fixed at material surface.
2, preparation method of the present invention is simple, and the coating being prepared can increase the bio-compatible on bone renovating material surface Property, improve hydrophilic effect, is suitble to promote and apply.
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, abstract), unless specifically stated, It is replaced by other equivalent or with similar purpose alternative features.That is, unless specifically stated, each feature is a series of An example in equivalent or similar characteristics.
Embodiment 1
A kind of preparation method of the surface coating of bone grafting material, comprising the following steps: into tetrahydrofuran solution according to quality Gelatin 2%, fibroin albumen 1%, the carbodiimides of hydroxyapatite 5% and 3% is added in percentage, and 45 DEG C of reaction 3h obtain finished product.
Embodiment 2
A kind of preparation method of the surface coating of bone grafting material, comprising the following steps: into toluene solution according to quality percentage Than gelatin 2.5%, fibroin albumen 1.2%, hydroxyapatite 6%, 4% carbodiimides is added, 60 DEG C of reaction 2.5h obtain finished product.
Embodiment 3
A kind of preparation method of the surface coating of bone grafting material, comprising the following steps: into diethyl ether solution according to quality percentage Than gelatin 3%, fibroin albumen 1%, hydroxyapatite 8%, 6% carbodiimides is added, 60 DEG C of reaction 2h obtain finished product.
Embodiment 4
A kind of preparation method of the surface coating of bone grafting material, comprising the following steps: into diethyl ether solution according to quality percentage Than gelatin 2%, fibroin albumen 1.5%, hydroxyapatite 8%, 5% chitosan is added, 60 DEG C of reaction 2.5h obtain finished product.
Embodiment 5
A kind of preparation method of the surface coating of bone grafting material, comprising the following steps: into formalin according to quality percentage Than gelatin 3%, fibroin albumen 1%, the chitosan of hydroxyapatite 7% and 3% is added, 67 DEG C of reaction 1.5h obtain finished product.
Embodiment 6
A kind of preparation method of the surface coating of bone grafting material, comprising the following steps: into acetaldehyde solution according to quality percentage Finished product is obtained than 750 DEG C of gelatin 2.5%, fibroin albumen 1.5%, the chitosan of hydroxyapatite 8% and 3% reaction 1.5h are added.
The invention is not limited to specific embodiments above-mentioned.The present invention, which expands to, any in the present specification to be disclosed New feature or any new combination, and disclose any new method or process the step of or any new combination.

Claims (7)

1. a kind of preparation method of the surface coating of bone grafting material, which comprises the following steps: by gelatin, fibroin Albumen, hydroxyapatite are sufficiently mixed by good solvent, and crosslinking agent is added, and reaction obtains finished product at a certain temperature.
2. the preparation method of the surface coating of bone grafting material according to claim 1, which is characterized in that according to quality hundred Divide than including gelatin 2 ~ 3%, fibroin albumen 1 ~ 1.5%, hydroxyapatite 5 ~ 8%.
3. the preparation method of the surface coating of bone grafting material according to claim 1, which is characterized in that described benign molten Agent includes one of tetrahydrofuran, ether, acetaldehyde, formaldehyde, toluene.
4. the preparation method of the surface coating of bone grafting material according to claim 1, which is characterized in that crosslinking agent carbonization Diimine.
5. the preparation method of the surface coating of bone grafting material according to claim 4, which is characterized in that the reaction temperature Degree is 45 ~ 70 DEG C.
6. the preparation method of the surface coating of bone grafting material according to claim 1, which is characterized in that crosslinking agent shell is poly- Sugar.
7. the preparation method of the surface coating of bone grafting material according to claim 6, which is characterized in that the reaction temperature Degree is 60 ~ 70 DEG C.
CN201811636068.3A 2018-12-29 2018-12-29 A kind of preparation method of the surface coating of bone grafting material Pending CN109621007A (en)

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Application Number Priority Date Filing Date Title
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102397584A (en) * 2011-11-15 2012-04-04 东南大学 Medical magnesium alloy surface degradable composite protective film layer and preparation method thereof
US20140219973A1 (en) * 2011-08-23 2014-08-07 Victoria L. Boyes Composite Hydrogel-Clay Particles
CN106540319A (en) * 2016-12-08 2017-03-29 苏州艾博迈尔新材料有限公司 A kind of medical nano biological coating and its preparation technology
CN106729991A (en) * 2016-11-22 2017-05-31 上海纳米技术及应用国家工程研究中心有限公司 A kind of segmentation coating for body implanting material
CN107761148A (en) * 2016-08-22 2018-03-06 张贞 A kind of method that fibroin albumen hydroxyapatite coating layer is prepared in metal surface

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140219973A1 (en) * 2011-08-23 2014-08-07 Victoria L. Boyes Composite Hydrogel-Clay Particles
CN102397584A (en) * 2011-11-15 2012-04-04 东南大学 Medical magnesium alloy surface degradable composite protective film layer and preparation method thereof
CN107761148A (en) * 2016-08-22 2018-03-06 张贞 A kind of method that fibroin albumen hydroxyapatite coating layer is prepared in metal surface
CN106729991A (en) * 2016-11-22 2017-05-31 上海纳米技术及应用国家工程研究中心有限公司 A kind of segmentation coating for body implanting material
CN106540319A (en) * 2016-12-08 2017-03-29 苏州艾博迈尔新材料有限公司 A kind of medical nano biological coating and its preparation technology

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A.BIGI等: "戊二醛不同程度交联明胶膜力学和热学性能", 《西部皮革》 *
WARD,A.G.等: "《明胶的科学与工艺学》", 31 March 1982, 轻工业出版社 *
关志宇主编: "《药物制剂辅料与包装材料》", 31 January 2017, 北京：中国医药科技出版社 *
徐孝旭著: "《无机纳米粒子 柞蚕丝素复合膜的制备、结构及性能研究》", 31 December 2012, 沈阳：辽宁科学技术出版社 *
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Application publication date: 20190416