CN109619597A - Mature vinegar nanoparticle-chelates of zinc preparation method - Google Patents
Mature vinegar nanoparticle-chelates of zinc preparation method Download PDFInfo
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- CN109619597A CN109619597A CN201910021557.6A CN201910021557A CN109619597A CN 109619597 A CN109619597 A CN 109619597A CN 201910021557 A CN201910021557 A CN 201910021557A CN 109619597 A CN109619597 A CN 109619597A
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- 229910052725 zinc Inorganic materials 0.000 title claims abstract description 103
- 239000011701 zinc Substances 0.000 title claims abstract description 103
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 title claims abstract description 97
- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 235000021419 vinegar Nutrition 0.000 title claims abstract description 29
- 239000000052 vinegar Substances 0.000 title claims abstract description 29
- 239000002105 nanoparticle Substances 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000006243 chemical reaction Methods 0.000 claims abstract description 16
- 239000000243 solution Substances 0.000 claims abstract description 15
- 239000007864 aqueous solution Substances 0.000 claims abstract description 13
- 239000008367 deionised water Substances 0.000 claims abstract description 7
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 7
- 239000007787 solid Substances 0.000 claims abstract description 7
- 238000004090 dissolution Methods 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 13
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 6
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 6
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims description 3
- 239000013522 chelant Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 239000004246 zinc acetate Substances 0.000 claims description 3
- 239000011592 zinc chloride Substances 0.000 claims description 3
- 235000005074 zinc chloride Nutrition 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 229960001763 zinc sulfate Drugs 0.000 claims description 3
- 235000013305 food Nutrition 0.000 abstract description 7
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 230000009967 tasteless effect Effects 0.000 abstract description 2
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 abstract 1
- 230000008569 process Effects 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 238000001255 X-ray photoelectron diffraction Methods 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- 238000004833 X-ray photoelectron spectroscopy Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000012512 characterization method Methods 0.000 description 4
- 239000003446 ligand Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000002189 fluorescence spectrum Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 238000002211 ultraviolet spectrum Methods 0.000 description 3
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 206010048259 Zinc deficiency Diseases 0.000 description 2
- 230000003542 behavioural effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
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- 229910052751 metal Inorganic materials 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- VCUVETGKTILCLC-UHFFFAOYSA-N 5,5-dimethyl-1-pyrroline N-oxide Chemical compound CC1(C)CCC=[N+]1[O-] VCUVETGKTILCLC-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 208000027219 Deficiency disease Diseases 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028813 Nausea Diseases 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 241000218636 Thuja Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000004630 atomic force microscopy Methods 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003710 cerebral cortex Anatomy 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000001320 hippocampus Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000000622 irritating effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- -1 zinc free radical Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
- A23L33/165—Complexes or chelates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of preparation methods of mature vinegar nanoparticle-chelates of zinc, nanoparticle-chelates of zinc is solid micro-yellow powder, it is tasteless, it is soluble easily in water, specific preparation process is as follows: A, taking 100-125 mg nanoparticle, it is dissolved in 10-25 mL deionized water, is configured to 5-10 mg mL‑1Nanoparticle aqueous solution；B, 50-62.5 mg inorganic zinc is taken to be added to 10-25mL, 5-10 mg mL‑1Nanoparticle aqueous solution in；C, inorganic zinc after completely dissolution, the chelatropic reaction 90-120 min under 60-90 DEG C of temperature of water bath condition；D, reaction solution is moved into 500 Da bag filters and is dialysed, solution is dry in bag taking, obtains nanoparticle-chelates of zinc solid powder.The present invention establishes the technology of nanoparticle and Zinc Ions Chelated preparation for the first time, obtains a kind of novel good nourishment intensifying agent with zinc of biocompatibility, and nanoparticle-chelates of zinc of acquisition can be used as the novel zinc-supplementing of one kind and apply in food.
Description
Technical field
The present invention relates to a kind of preparation methods of mature vinegar nanoparticle-chelates of zinc.
Background technique
In process, organic molecule and oligomer in raw material are heated to occur series reaction to food, this with hydro-thermal/
The process of solvent structure nanoparticle is similar, and mature vinegar is mainly gelatinized by raw materials such as sorghum, yeast, wheat bran, Gu Kang, is sent out
The techniques such as ferment, smoked unstrained spirits are brewed, and there is the process inevitably heated to raw material in process of production, in this process
In, protein and polysaccharide in cereal materials are degraded to short peptide chain, amino acid and oligosaccharides, mutually occur between these substances anti-
It answers, forms the nanoparticle in mature vinegar, this food-borne nanoparticle biological safety height and good biocompatibility.
Zn-ef ficiency is required microelement in animal body, and the enzyme containing metallic zinc is widely distributed in human body, is had
Studies have shown that zinc is the important component of 40 various metals enzymes in human body, and related with the activity of more than 30 kinds of enzymes, Zn-ef ficiency
Body eubolism and growth and development are also maintained, zinc mainly participates in the conjunction of nucleic acid and protein in human body by a variety of enzymes
At metabolic processes such as, energetic supersession, redox, and then influences the metabolism of body and growth and development, zinc-deficiency and can reduce core
The activity of thuja acid synzyme, thymidylic acid enzyme, archaeal dna polymerase and RNA polymerase sends out the synthesis of nucleic acid and protein
Raw obstacle is interfered so as to cause body cell division and growth, Zn-ef ficiency content highest in human brain, especially with hippocampus
With cerebral cortex layer content highest, and the two and behavioral function are closely related, thus the distribution of zinc in brain can react its
The important function in behavioral function is adjusted, when zinc-deficiency, the stability of cell membrane changes in brain, and brain cell is by serious
Damage, brain development is obstructed, and memory level also will receive influence, although zinc source very abundant in nature, zinc is in people
Intracorporal Absorption And Metabolism is limited by many factors；In order to prevent and treat and alleviate the generation of Zn-ef ficiency deficiency disease, every country
Area all strengthens Zn-ef ficiency in food according to the actual situation, currently, Zn-ef ficiency supplement mainly passes through two kinds of approach: first is that using rich
The food of Zn-ef ficiency is supplemented；Second is that being supplemented by taking supplementing preparation, supplemented using the food of zinc-rich element,
Due to the finiteness of its constituent content, body can not be fully met to the needs of metallic element；Therefore, people mainly utilize benefit
It fills agent to be supplemented, Zn-ef ficiency supplementing preparation comes into being in this context, is developed so far from first generation Zn-ef ficiency replenishers,
Through changing after four times, first generation Zn-ef ficiency replenishers are inorganic salt types, and manufacture craft is simple, and production cost is low, however
Biological absorption is lower, and to the irritating effect of gastrointestinal tract；Second generation Zn-ef ficiency replenishers are to do ligand by organic acid to synthesize
Preparation, good absorbing, but preparation process are complicated, have the undesirable clinical response such as nauseous constipation, are also unsuitable for the spies such as diabetes patient
Different group；Third generation Zn-ef ficiency replenishers are the preparations that ligand synthesis is done with amino acid, and it is steady not to be only soluble in water also chemical property
Fixed, absorption rate in vivo is also higher, but there is also certain defects in terms of chelation mechanism and operating；Forth generation zinc member
Plain replenishers are the preparations that ligand synthesis is done with peptide, are also equipped with one except the advantages of making the preparation of ligand synthesis with amino acid
Fixed antioxidant activity, but the hydrolysis of control peptide and the production technology of purity are still not perfect.
The Zn-ef ficiency nutritional supplement final product quality of prior art production is unstable, some product tastes are bad, absorptivity
It is lower and expensive, these problems all urgent need to resolve, nanoparticle surface functional group rich in, and have good
Water solubility, using food-borne nanoparticle as carrier carry out Zn-ef ficiency nutrient delivery, have good biocompatibility and
Higher biological safety.
Summary of the invention
The object of the present invention is to provide it is a kind of for the first time using in mature vinegar naturally occurring nanoparticle as delivery vector, nanometer
Particle-chelates of zinc can be added in food, realize nanoparticle-chelates of zinc preparation side of the nutritional supplementation of Zn-ef ficiency
Method.
Present invention technical solution used for the above purpose is: a kind of 1. mature vinegar nanoparticles-chelates of zinc
Preparation method, which comprises the following steps:
A, the nanoparticle for taking certain mass, is dissolved in deionized water, the nanoparticle aqueous solution being configured to；
B, in the nanoparticle aqueous solution for taking the inorganic zinc of certain mass to be added to；
C, after completely dissolution, chelatropic reaction is for a period of time under the water bath condition of certain temperature for inorganic zinc；
D, reaction solution is moved into bag filter and is dialysed, solution is dry in bag taking, obtains nanoparticle-zinc
Chelate solid powder.
The quality of nanoparticle is 100-125 mg in the step A, is dissolved in 10-25 mL deionized water, is prepared
At 5-10 mg mL-1Nanoparticle aqueous solution.
The quality of inorganic zinc is 50-62.5 mg in the step B, is added to 10-25mL, 5-10 mg mL-1Nanoparticle
In sub- aqueous solution.
Inorganic zinc is one or more of zinc sulfate, zinc chloride, zinc nitrate and zinc acetate in the step B.
The mass ratio of nanoparticle and inorganic zinc is 2:1 in the step B.
In the step C inorganic zinc after completely dissolution, the chelatropic reaction 90-120 under 60-90 DEG C of temperature of water bath condition
min。
Reaction solution is moved into 500Da bag filter in the step D and is dialysed.
Drying means is the knot of one of vacuum freeze drying and vacuum dehydrating at lower temperature or two kinds of methods in the step D
It closes.
A kind of preparation method of mature vinegar nanoparticle-chelates of zinc of the present invention, required raw material are inexpensively easily purchased；Used receives
Rice corpuscles has been formed in mature vinegar process, without artificially being synthesized；Mature vinegar nano-particles size is small, has good
Water-soluble and biocompatibility；Nanoparticle-chelates of zinc preparation method simple process and low cost；Nanoparticle-zinc chelating
Object good mouthfeel is easy to absorb；Nanoparticle-chelates of zinc has high safety.
Detailed description of the invention
Fig. 1 is a kind of preparation method nanoparticle-chelates of zinc atom of mature vinegar nanoparticle-chelates of zinc of the present invention
The picture of force microscope.
Fig. 2 is that a kind of preparation method nanoparticle-chelates of zinc X- of mature vinegar nanoparticle-chelates of zinc of the present invention is penetrated
The map of Photoelectron diffraction (XRD).
Fig. 3 is a kind of preparation method nanoparticle-chelates of zinc fluorescence of mature vinegar nanoparticle-chelates of zinc of the present invention
Spectrum.
Fig. 4 is that a kind of preparation method nanoparticle-chelates of zinc of mature vinegar nanoparticle-chelates of zinc of the present invention is ultraviolet
Spectrum.
Fig. 5 is in a kind of preparation method nanoparticle-chelates of zinc Fu of mature vinegar nanoparticle-chelates of zinc of the present invention
Leaf transformation infrared spectroscopy (FT-IR) map.
Fig. 6 is that a kind of preparation method nanoparticle-chelates of zinc X- of mature vinegar nanoparticle-chelates of zinc of the present invention is penetrated
The map of photoelectron spectra (XPS).
Fig. 7 is a kind of preparation method nanoparticle-chelates of zinc hydroxyl of mature vinegar nanoparticle-chelates of zinc of the present invention
Free radical scavenging ability map.
Fig. 8 is a kind of preparation method nanoparticle-chelates of zinc hydroxyl free of mature vinegar nanoparticle-chelates of zinc of the present invention
Base Scavenging activity map.
Specific embodiment
Mature vinegar nanoparticle-chelates of zinc preparation method, nanoparticle-chelates of zinc are solid micro-yellow powder,
Tasteless, soluble easily in water, specific preparation process is as follows: A, taking 100-125 mg nanoparticle, is dissolved in 10-25 mL deionization
In water, it is configured to 5-10 mg mL-1Nanoparticle aqueous solution；B, 50-62.5 mg inorganic zinc is taken to be added to 10-25mL, 5-
10 mg mL-1Nanoparticle aqueous solution in；C, inorganic zinc after completely dissolution, chelates under 60-90 DEG C of temperature of water bath condition
React 90-120 min；D, reaction solution is moved into 500 Da bag filters and is dialysed, solution is dry in bag taking, obtains nanoparticle-
Chelates of zinc solid powder, inorganic zinc are one or more of zinc sulfate, zinc chloride, zinc nitrate and zinc acetate, nanoparticle
Mass ratio with inorganic zinc is 2:1, and drying means is one of vacuum freeze drying and vacuum dehydrating at lower temperature or two kinds of methods
Combination, nanoparticle-chelates of zinc for being formed can be used as nutrition fortifier in food after nanoparticle and inorganic zinc chelating
Using.
Embodiment 1, the extraction and the preparation of nanoparticle-chelates of zinc of nanoparticle
It takes dry nanoparticle 100-125 mg that 10-25 mL deionized water is added sufficiently to dissolve, is formulated as 5-10 mg mL-1
Solution, add the inorganic zinc of 50-62.5 mg, the volume ratio both in system is 2:1, and 90 DEG C of perseverances are kept after being completely dissolved
Temperature is sufficiently stirred under acting on reacts 120 min, reaction solution 500 Da bag filters, 4 h of dialysis, and solution freeze-drying in bag taking is received
Collect solid nanoparticles-chelates of zinc.
Embodiment 2, nanoparticle-chelates of zinc property representation
S1, nanoparticle-chelates of zinc morphological feature, Figure 1A are nanoparticle-chelates of zinc atomic force microscopy mirror planes
Figure, Figure 1B is nanoparticle-chelates of zinc three-dimensional imaging figure, and Fig. 1 C is nanoparticle-chelates of zinc grain size distribution, knot
Fruit shows that for nanoparticle-chelates of zinc form rule of preparation at nearly ball-type, statistics obtains nanoparticle-chelates of zinc partial size
Size concentrates on 10 nm or so；S2, nanoparticle-chelates of zinc x ray photoelectron diffraction (XRD) experiment, Fig. 2 is to receive
Rice corpuscles-chelates of zinc XRD spectrum, it is seen that 2 theta=22.88oPlace only has a steamed bun peak, according to XRD spectrum, explanation
Nanoparticle-the chelates of zinc has unbodied carbon structure；S3, nanoparticle-chelates of zinc fluorescence spectrum characterization, Fig. 3
It is nanoparticle-chelates of zinc fluorescence spectrum, it is visible with excitation wavelength by nanoparticle-chelates of zinc fluorescence spectrum
Increase launch wavelength and apparent Red Shift Phenomena occur, nanoparticle-chelates of zinc maximum excitation wavelength appears in 340 nm
Place, corresponding launch wavelength are 426 nm；S4, nanoparticle-chelates of zinc ultraviolet spectra characterization, Fig. 4 is nanoparticle-zinc
The ultraviolet spectra of chelate occurs the characteristic absorption peak of n → π * transition in 278 nm by ultraviolet spectrogram is visible；S5, it receives
Rice corpuscles-chelates of zinc fluorescence lifetime, Fig. 5 are nanoparticle-chelates of zinc fluorescence lifetime maps.Configure 2mg mL-1's
Nanoparticle-chelates of zinc aqueous solution, excites under the exciting light of 340 nm, and maximum emission peak is 426 nm, measures the fluorescence longevity
Life, through experiments, it was found that nanoparticle-chelates of zinc is the quenching of two fingers number, through the Fitting Calculation nanoparticle-chelates of zinc fluorescence
Service life is 6.29 ns；S6, nanoparticle-chelates of zinc Fourier transform infrared spectroscopy (FT-IR) characterization, Fig. 6 is nanoparticle
Son-chelates of zinc infared spectrum, the result shows that nanoparticle-chelates of zinc of preparation is in 3428 cm in figure-1Contain O-H's
Stretching vibration peak, 2937 cm-1There are the stretching vibration peak of C-H, 1722 cm in place-1There are the stretching vibration peak of C=O, 1640 cm-1There is C
The stretching vibration peak of=C, 1438 cm-1There are the deformation vibration peak of C-N, 1050 cm-1There are the stretching vibration peak of C-O-C, S7, nanometer
Particle-chelates of zinc x-ray photoelectron spectroscopy (XPS) characterization, Fig. 7 is the map of mature vinegar nanoparticle XPS, result table in figure
Mainly contain two kinds of elements of C and O and a small amount of N element in bright nanoparticle-chelates of zinc, and there are a certain amount of Zn element,
Each element content in 1 nanoparticle of table-chelates of zinc xps energy spectrum
。
Embodiment 3, inoxidizability experiment
By sample preparation at solution, it is diluted to certain concentration gradient, reaction system is configured: being separately added into 38 μ L thereto
PBS (pH=7.4) buffer solution of 0.15 mol L-1,39 μ L sample solution, 56 mmol L-1 of μ L DMPO, 10 μ L
EDTA-2Na-FeSO4, be eventually adding 8 μ L H2O2 (6%) starting reaction.Mixed liquor is uniformly mixed and is placed on 40 DEG C of water-baths
Sample solution is replaced with deionized water by 30 min of middle reaction, control group, measures hydroxyl using electronic paramagnetic resonance spectrometer (ESR)
Base free radical production, Fig. 8 are that nanoparticle-chelates of zinc free radical generates and removes feelings under various concentration gradient
Condition, nanoparticle-chelates of zinc does not lead to the generation of free radical according to the experimental results.
Claims (8)
1. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc, which comprises the following steps:
The nanoparticle for taking certain mass, is dissolved in deionized water, the nanoparticle aqueous solution being configured to；
In the nanoparticle aqueous solution for taking the inorganic zinc of certain mass to be added to；
After completely dissolution, chelatropic reaction is for a period of time under the water bath condition of certain temperature for inorganic zinc；
Reaction solution is moved into bag filter and is dialysed, solution is dry in bag taking, obtains nanoparticle-zinc
Chelate solid powder.
2. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc according to claim 1, it is characterised in that: described
The quality of nanoparticle is 100-125 mg in step A, is dissolved in 10-25 mL deionized water, is configured to 5-10 mg mL-1
Nanoparticle aqueous solution.
3. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc according to claim 1, it is characterised in that: described
The quality of inorganic zinc is 50-62.5 mg in step B, is added to 10-25mL, 5-10 mg mL-1Nanoparticle aqueous solution in.
4. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc according to claim 1, it is characterised in that: described
Inorganic zinc is one or more of zinc sulfate, zinc chloride, zinc nitrate and zinc acetate in step B.
5. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc according to claim 1, it is characterised in that: described
The mass ratio of nanoparticle and inorganic zinc is 2:1 in step B.
6. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc according to claim 1, it is characterised in that: described
In step C inorganic zinc after completely dissolution, the chelatropic reaction 90-120 min under 60-90 DEG C of temperature of water bath condition.
7. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc according to claim 1, it is characterised in that: described
Reaction solution is moved into 500Da bag filter in step D and is dialysed.
8. a kind of preparation method of mature vinegar nanoparticle-chelates of zinc according to claim 1, it is characterised in that: described
Drying means is the combination of one of vacuum freeze drying and vacuum dehydrating at lower temperature or two kinds of methods in step D.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498863A (en) * | 2021-07-02 | 2021-10-15 | 大连工业大学 | Preparation method and application of nano-carrier with free radical scavenging capability |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494214A (en) * | 2013-09-29 | 2014-01-08 | 安徽农业大学 | Casein phosphopeptide and zinc chelate compound |
| CN105012940A (en) * | 2015-07-13 | 2015-11-04 | 国家海洋局第三海洋研究所 | Preparation method of nanometer collagen peptide zinc chelate |
| CN106699319A (en) * | 2015-07-29 | 2017-05-24 | 宋佰会 | Nanocarbon plant nutrient solution and preparation method thereof |
| CN107603610A (en) * | 2017-09-14 | 2018-01-19 | 大连工业大学 | Preparation method from mature vinegar carbon nano-particles |
| CN108059148A (en) * | 2017-12-28 | 2018-05-22 | 大连工业大学 | The preparation method of nano-particle with Scavenging ability |
| CN108273071A (en) * | 2018-01-26 | 2018-07-13 | 大连工业大学 | A kind of food-borne fluorescence nano grain and its preparation method and application |
| CN108669312A (en) * | 2018-05-21 | 2018-10-19 | 河南广安生物科技股份有限公司 | Small-peptide chelated zinc of a kind of nanometer for feed addictive and preparation method thereof |
-
2019
- 2019-01-10 CN CN201910021557.6A patent/CN109619597A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103494214A (en) * | 2013-09-29 | 2014-01-08 | 安徽农业大学 | Casein phosphopeptide and zinc chelate compound |
| CN105012940A (en) * | 2015-07-13 | 2015-11-04 | 国家海洋局第三海洋研究所 | Preparation method of nanometer collagen peptide zinc chelate |
| CN106699319A (en) * | 2015-07-29 | 2017-05-24 | 宋佰会 | Nanocarbon plant nutrient solution and preparation method thereof |
| CN107603610A (en) * | 2017-09-14 | 2018-01-19 | 大连工业大学 | Preparation method from mature vinegar carbon nano-particles |
| CN108059148A (en) * | 2017-12-28 | 2018-05-22 | 大连工业大学 | The preparation method of nano-particle with Scavenging ability |
| CN108273071A (en) * | 2018-01-26 | 2018-07-13 | 大连工业大学 | A kind of food-borne fluorescence nano grain and its preparation method and application |
| CN108669312A (en) * | 2018-05-21 | 2018-10-19 | 河南广安生物科技股份有限公司 | Small-peptide chelated zinc of a kind of nanometer for feed addictive and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113498863A (en) * | 2021-07-02 | 2021-10-15 | 大连工业大学 | Preparation method and application of nano-carrier with free radical scavenging capability |
| CN113498863B (en) * | 2021-07-02 | 2024-01-26 | 大连工业大学 | Preparation method and application of nano-carrier with free radical removal capability |
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Application publication date: 20190416 |