CN109613246A - A kind of chip and preparation method thereof detecting four kinds of Thyroid tumor markers - Google Patents
A kind of chip and preparation method thereof detecting four kinds of Thyroid tumor markers Download PDFInfo
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- CN109613246A CN109613246A CN201910022561.4A CN201910022561A CN109613246A CN 109613246 A CN109613246 A CN 109613246A CN 201910022561 A CN201910022561 A CN 201910022561A CN 109613246 A CN109613246 A CN 109613246A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
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Abstract
The invention discloses the protein chip that one kind can detect four kinds of Common Thyroid tumor markers concentration simultaneously, the chip is made as follows: (1) black slide pretreatment；(2) point sample captures antibody component solution；(3) chip is closed, the chip is made.Chip of the present invention is used to detect the concentration of Thyroid tumor markers in the blood of patient, thus judges the recurrence of thyroid tumors after whether patient obtained thyroid tumors or judgement operation.The kit that this method is formed is easy to use, and recall rate is high, but also the full-automatic operation of Thyroid tumor markers detection may be implemented.
Description
Technical field
The present invention relates to field of biotechnology, more particularly, to one kind for detecting four kinds of Thyroid tumor markers
The chip and preparation method thereof of (Ctn, Tg, TSH and CEA) concentration.
Background technique
Thyroid gland is located at below neck thyroid cartilage, tracheae both sides, and shape is like butterfly, like shield first, so claiming
Be thyroid gland.Thyroid gland is divided into two leaves of left and right and isthmus.Two leaves of left and right are located at the two sides of hypolarynx and organ top.Upper end
From thyroid midpoint, lower end to the 6th tracheal rings, sometimes up to after suprasternal fossa or breastbone.
Thyroid gland has synthesis, storage and the function of secreting thyroxine, and structural units is folliculus.Thyroxine is a kind of
Combination iodine containing iodotyrosine has two kinds of four iodotyrosines (T4) and Triiodotyrosine (T3), after synthesis just with first
Shape gland globulin combines, and is stored in thyroid follicle.The thyroxine of blood is released into conjunction with haemocyanin, wherein 90% is
T4,10% is T3.The main function of thyroxine is: accelerating the efficiency that systemic cell utilizes oxygen, accelerates protein, carbon aquation
The metabolism of human body is increased in the decomposition for closing object and fat comprehensively, is increased heat and is generated；, promote the growth and development of human body, mainly going out
Brain and long bone are influenced after life.
Treatment of Benign Tumor of Thyroid Gland is very common, and in neck region lump, thyroid adenoma accounts for about 50%.General non-evident sympton, when
When knurl is larger, can because tracheas suppressed, oesophagus, nerve and lead to the symptoms such as expiratory dyspnea, dysphagia, hoarseness, when
Tumour can generate local distending pain when merging bleeding and increasing rapidly.Because Treatment of Benign Tumor of Thyroid Gland has, canceration is possible, although a part
It is benign, but is in " hot nodule " (i.e. high functionality), so needs active treatment.
In thyroid malignancy most commonly thyroid cancer (Thyroid carcinoma), only a few can have pernicious
Lymthoma and metastatic tumor, thyroid cancer account for the 1% of whole body malignant tumour.In addition to cephaloma, most thyroid cancers originate from
Follicular epithelial cells.The disease incidence of thyroid cancer and area, race, sex have certain relationship.The thyroid cancer incidence in the U.S.
It is higher, according to statistics, between 1973-2002, the Annual occurence rate of U.S.'s thyroid cancer increases to 100,000 by 3.6/100000ths/
8.7, it has increased about 2.4 times (P < 0.001), and this trend is still increasing year by year.Domestic thyroid cancer incidence
It is lower, according to statistics, wherein male about 0.8-0.9/10 ten thousand, women about 2.0-2.2/10 ten thousand.
Clinical labororatory's detection of thyroid cancer blood serum designated object mostly uses immunological detection method, including thyroid gland at present
Globulin (thyroglobulin, Tg) and calcitonin (calcitonin, Ctn).Ctn is medullary carcinoma of thyroid gland serum detection mark
Will object, Tg can become differentiated thyroid carcinoma serum and detect marker.Patients with thyroid neoplasm such as serum thyroid stimulating hormone
(thyroid stimulating hormone, TSH) is horizontal lower than normally, and tubercle is that pernicious ratio is being lower than TSH level just
Often or increase.Therefore TSH level has reference significance for the assessment of malignant tumour to a certain extent.Carcinomebryonic antigen
There are related for (carcinoembryonicantigen, CEA) and the diagnosis of part medullary carcinoma of thyroid gland patient and clinical progress
Property, the serum measurement of medullary carcinoma of thyroid gland can be applied to together with Ctn1。
Common detection method is generally Elisa or chemoluminescence method and is surveyed one by one to these indexs on the market at present
It is fixed.Elisa haves the shortcomings that the low range of linearity of sensitivity is narrow, and chemiluminescence hair has that at high cost, flux is small.
Summary of the invention
In view of the above-mentioned problems existing in the prior art, the applicant provides a kind of disposably four kinds of common first of detection
The chip and preparation method thereof of shape adenoncus tumor markers.For chip of the present invention for detecting Thyroid tumor markers, recall rate is high,
No pain, no risk is at low cost, easy to operate, only a small amount of blood is needed just to can detect many a projects, and also achieve first shape
The full-automatic operation of adenoncus tumor markers detection.
Technical scheme is as follows:
Chip of the present invention is made as follows:
(1) black slide pretreatment；
(2) point sample captures antibody component solution；
(3) chip is closed, the chip is made.
The black pretreated method of slide described in step (1) are as follows:
1. by black slide be placed in the slide pretreatment fluid containing NaOH impregnate 16~for 24 hours, later using purified water cleaning 2
~8 times；
2. black slide is placed in the solution of silane that mass concentration is 0.05~1wt% and impregnates 20~60min；
3. 0.2 is toasted under the conditions of 100~180 DEG C by being put into baking oven after soaked black slide nitrogen purging~
0.6h。
Capture antibody component solution described in step (2) includes the capture antibody component of calcitonin, Tg, TSH, CEA
Solution.
The method of point sample described in step (2) is Machine automated point sample, on black slide o'clock at 4 × 7 matrix；Each column
The point formed for same capture antibody component solution.
Closed process described in step (3) are as follows: the good black slide of point sample is submerged into 1~4h in confining liquid, is taken out later black
Slide, and it is centrifuged the remaining confining liquid of removal, the chip is made.
The confining liquid is the buffer solution containing closed protein；The closed protein is bovine serum albumin(BSA) or egg white egg
It is white；The buffer is one of PBS buffer solution, Tris buffer, HEPS buffer, MOPS buffer or a variety of.
A kind of application of the chip, is made kit for the chip.
The kit further includes the secondary antibody solution for being marked with HRP enzyme or alkali phosphorus enzyme, detection liquid A and detection liquid B.Institute
It states detection liquid A and contains 1% luminol and 2%Tris, detection liquid B contains 1% hydrogen peroxide.
Detection basic principle of the invention are as follows:
The immunological method that product of the present invention detects Thyroid tumor markers is double-antibody method, and capture therein is anti-
It is fixed on carrier matrix according to specific lattice array with micro-liquid distributer, these antibody can capture special in tested sample
Anisotropic antigen, captured antigen can mutually be tied again with second antibody for being marked with horseradish peroxidase or alkali phosphorus enzyme
It closes, horseradish peroxidase or alkali phosphorus enzymatic chemiluminescent substrate, optical signal are acquired by CCD camera, and carry out intelligence
It can analysis.According to signal concentration quantitative curve, pass through the strong and weak concentration for calculating microorganism in tested sample of optical signal.
The present invention is beneficial to be had the technical effect that
This method is intensive style for the first time, the method for detecting multiple Thyroid tumor markers simultaneously with high throughput.This skill
Art obtains the sensitivity of height using chemiluminescence.This method is available with full-automation there are one great advantages
Chip reading instrument, realize high speed, easy detection needs a large amount of manually operated methods to be more suitable hospital a large amount of than other
Carry out.
For the present invention compared with external import reagent (Roche Diagnistics), the recall rate height of Thyroid tumor markers is consistent,
It is functional.
Detailed description of the invention
Fig. 1 is that antibody component point sample schematic diagram is captured in preparation method of the present invention.
In figure, column 1: positive quality control column；Column 2 and column 6: blank；It arranges 3:calcitonin and captures antibody；It is anti-to arrange 4:Tg capture
Body column；Arrange 5:TSH capture antibody column；Arrange 6:CEA capture antibody column；Column 7: negative Quality Control column.
Specific embodiment
With reference to the accompanying drawings and examples, the present invention is specifically described.
Embodiment 1
A kind of chip detecting Common Thyroid tumor markers, the chip are made as follows:
(1) black slide pretreatment；
1. black slide is placed in the slide pretreatment fluid containing NaOH and impregnates 16h, later using purified water cleaning 2~8
It is secondary；
2. black slide is placed in the solution of silane (medium is 25% ethyl alcohol) that mass concentration is 1wt% and impregnates 20min；
3. toasting 0.2h under the conditions of 180 DEG C for being put into baking oven after soaked black slide nitrogen purging.
(2) point sample captures antibody component solution；
Referring to Fig.1, using Machine automated point sample, on black slide o'clock at 4 × 7 matrix；It is anti-to be often classified as same capture
The point that body component solution is formed；The capture antibody component solution includes the capture antibody group of calcitonin, Tg, TSH, CEA
Divide solution.
(3) closing capture antibody component solution；
The good black slide of point sample is submerged into 3h, Zhi Houqu in confining liquid (the Tris buffer containing 3% bovine serum albumin(BSA))
Black slide out, and it is centrifuged the remaining confining liquid of removal, the chip is made.
(4) kit
By chip and the secondary antibody solution (calcitonin, Tg, TSH and CEA antibody), the detection liquid A that are marked with HRP enzyme
(containing 1% luminol and 2%Tris), detection liquid B (1% hydrogen peroxide) are packaged into kit jointly.
Embodiment 2
A kind of chip detecting Common Thyroid tumor markers, the chip are made as follows:
(1) black slide pretreatment；
It is impregnated for 24 hours 1. being placed in black slide in the slide pretreatment fluid containing NaOH, later using purified water cleaning 2~8
It is secondary；
It is impregnated 2. black slide is placed in the solution of silane (medium is 25% ethyl alcohol) that mass concentration is 0.05wt%
60min；
3. toasting 0.4h under the conditions of 140 DEG C for being put into baking oven after soaked black slide nitrogen purging.
(2) point sample captures antibody component solution；
Referring to Fig.1, using Machine automated point sample, on black slide o'clock at 4 × 7 matrix；It is anti-to be often classified as same capture
The point that body component solution is formed；The capture antibody component solution includes the capture antibody group of calcitonin, Tg, TSH, CEA
Divide solution.
(3) closing capture antibody component solution；
The good black slide of point sample is submerged into 4h in confining liquid (PBS buffer solution containing 6% ovalbumin), takes out black glass later
Piece, and it is centrifuged the remaining confining liquid of removal, the chip is made.
(4) kit
By chip and the secondary antibody solution (calcitonin, Tg, TSH and CEA antibody), the detection liquid A that are marked with HRP enzyme
(containing 1% luminol and 2%Tris), detection liquid B (containing 1% hydrogen peroxide) are packaged into kit jointly.
Embodiment 3
A kind of chip detecting Common Thyroid tumor markers, the chip are made as follows:
(1) black slide pretreatment；
1. black slide is placed in the slide pretreatment fluid containing NaOH and impregnates 20h, later using purified water cleaning 2~8
It is secondary；
It is impregnated 2. black slide is placed in the solution of silane (medium is 25% ethyl alcohol) that mass concentration is 0.5wt%
30min；
3. toasting 0.6h under the conditions of 100 DEG C for being put into baking oven after soaked black slide nitrogen purging.
(2) point sample captures antibody component solution；
Referring to Fig.1, using Machine automated point sample, on black slide o'clock at 4 × 7 matrix；It is anti-to be often classified as same capture
The point that body component solution is formed；The capture antibody component solution includes the capture antibody group of calcitonin, Tg, TSH, CEA
Divide solution.
(3) closing capture antibody component solution；
The good black slide of point sample is submerged into 1h, Zhi Houqu in confining liquid (the MOPS buffer containing 4% bovine serum albumin(BSA))
Black slide out, and it is centrifuged the remaining confining liquid of removal, the chip is made.
(4) kit
By chip and the secondary antibody solution (calcitonin, Tg, TSH and CEA antibody), the detection liquid A that are marked with HRP enzyme
(containing 1% luminol and 2%Tris), detection liquid B (containing 1% hydrogen peroxide) are packaged into kit jointly.
Test case:
Clinical serum is detected using the SLXP-001 type biological chip reading apparatus that our company produces, SLXP-001
The course of work of type biological chip reading apparatus is as follows:
The test serum sample of instrument automatic sucking 200ul is into reaction cup, and instrument is by egg made from the embodiment of the present invention
White chip is automatically put into test serum, and 37 DEG C are incubated for 40 minutes, and subsequent instrument clamping jaw takes out chip, rushes automatically through instrument
The secondary antibody solution (200ul, instrument are inhaled in advance automatically) for being marked with HRP enzyme is put into after washing, and is incubated for again after forty minutes,
Instrument clamping jaw takes out chip again, is put into luminous substrate solution after instrument auto-flushing (by the detection liquid A of 100ul
Mixed with the detection liquid B of 100ul, by instrument automatic sucking and mixing), imaging of taking pictures finally is carried out to protein chip, it is soft
Part automatically analyzes picture, provides analysis result.Using the testing result of external import reagent (Roche Diagnistics) as reference value.Detection knot
Fruit is as shown in table 1.
Table 1
As seen from the above table, the chip of detection Thyroid tumor markers provided by the present invention and internationally famous manufacturer's Roche
It is similar (relative error majority is within 10%) to diagnose comparison result, in sensitivity, the range of linearity etc. is also without obvious poor
It is different.High efficiency, letter operation may be implemented using the chip of detection Thyroid tumor markers provided by the invention, low cost is used
When multiple advantages such as short, with good application prospect.
Bibliography
" 1. thyroid cancer blood serum designated object clinical application Consensus of experts (2017 editions) ".
Claims (9)
1. a kind of rapid, the diagnosing chip of complete detection thyroid tumors can disposably detect four kinds of Common Thyroid tumours
Marker, it is characterised in that the chip is made as follows:
(1) black slide pretreatment；
(2) point sample captures antibody component solution；
(3) chip is closed, the chip is made.
2. chip according to claim 1, it is characterised in that the black pretreated method of slide described in step (1) are as follows:
1. by black slide be placed in the slide pretreatment fluid containing NaOH impregnate 16 ~ for 24 hours, later using purified water clean 2 ~ 8 times；
2. black slide is placed in the solution of silane that mass concentration is 0.05 ~ 1wt% and impregnates 20 ~ 60min；
3. toasting 0.2 ~ 0.6h under the conditions of 100 ~ 180 DEG C for being put into baking oven after soaked black slide nitrogen purging.
3. chip according to claim 1, it is characterised in that capturing antibody component solution described in step (2) includes
Calcitonin, Tg, the capture antibody component solution of TSH, CEA.
4. chip according to claim 1, it is characterised in that the method for point sample described in step (2) is Machine automated point
Sample, on black slide o'clock at 4 × 7 matrix；Often it is classified as the point that same capture antibody component solution is formed.
5. chip according to claim 1, it is characterised in that closed process described in step (3) are as follows: by good black of point sample
Slide submerges 1 ~ 4h in confining liquid, takes out black slide later, and is centrifuged the remaining confining liquid of removal, and the chip is made.
6. chip according to claim 5, it is characterised in that the confining liquid is the buffer solution containing closed protein；Institute
Stating closed protein is bovine serum albumin(BSA) or ovalbumin；The buffer is PBS buffer solution, Tris buffer, HEPS buffering
One of liquid, MOPS buffer are a variety of.
7. a kind of application of any one of claim 1 ~ 6 chip, it is characterised in that kit is made in the chip.
8. application according to claim 7, it is characterised in that the kit further includes being marked with HRP enzyme or alkali phosphorus enzyme
Secondary antibody solution, detection liquid A and detection liquid B.
9. application according to claim 8, it is characterised in that the detection liquid A contains 1% luminol and 2% Tris, detection
Liquid B contains 1% hydrogen peroxide.
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| CN201910022561.4A CN109613246A (en) | 2019-01-10 | 2019-01-10 | A kind of chip and preparation method thereof detecting four kinds of Thyroid tumor markers |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110596398A (en) * | 2019-08-14 | 2019-12-20 | 南京浦光生物科技有限公司 | Protein chip for detecting blood coagulation marker and preparation method and application thereof |
| CN112162050A (en) * | 2019-10-25 | 2021-01-01 | 北京大学 | Application of MIT and/or DIT as thyroid cancer marker and kit |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101377512A (en) * | 2008-04-16 | 2009-03-04 | 北京科美东雅生物技术有限公司 | Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin |
| CN101821628A (en) * | 2007-08-30 | 2010-09-01 | Ait奥地利技术研究所有限公司 | Set of tumour-markers |
| CN103869068A (en) * | 2012-12-18 | 2014-06-18 | 广州瑞博奥生物科技有限公司 | Antibody chip kit for diagnosis of various tumors |
| CN108387750A (en) * | 2018-02-08 | 2018-08-10 | 江苏三联生物工程有限公司 | A kind of chip and preparation method thereof of joint-detection F-T3, F-T4 and TSH |
| CN108753569A (en) * | 2018-05-25 | 2018-11-06 | 苏州博福生物医药科技有限公司 | A kind of enrichment isolation mechanism for capturing cell or biomolecule |
-
2019
- 2019-01-10 CN CN201910022561.4A patent/CN109613246A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101821628A (en) * | 2007-08-30 | 2010-09-01 | Ait奥地利技术研究所有限公司 | Set of tumour-markers |
| CN101377512A (en) * | 2008-04-16 | 2009-03-04 | 北京科美东雅生物技术有限公司 | Chemiluminescence immune analysis determination reagent kit for detecting thyroglobulin |
| CN103869068A (en) * | 2012-12-18 | 2014-06-18 | 广州瑞博奥生物科技有限公司 | Antibody chip kit for diagnosis of various tumors |
| CN108387750A (en) * | 2018-02-08 | 2018-08-10 | 江苏三联生物工程有限公司 | A kind of chip and preparation method thereof of joint-detection F-T3, F-T4 and TSH |
| CN108753569A (en) * | 2018-05-25 | 2018-11-06 | 苏州博福生物医药科技有限公司 | A kind of enrichment isolation mechanism for capturing cell or biomolecule |
Non-Patent Citations (1)
| Title |
|---|
| 编辑: "甲状腺球蛋白与降钙素检测改善甲状腺癌患者诊疗", 《国际检验医学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110596398A (en) * | 2019-08-14 | 2019-12-20 | 南京浦光生物科技有限公司 | Protein chip for detecting blood coagulation marker and preparation method and application thereof |
| CN112162050A (en) * | 2019-10-25 | 2021-01-01 | 北京大学 | Application of MIT and/or DIT as thyroid cancer marker and kit |
| CN112162050B (en) * | 2019-10-25 | 2022-01-11 | 北京大学 | Application of MIT and/or DIT as thyroid cancer marker and kit |
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Application publication date: 20190412 |