CN109612986A - Test paper, manufacturing method and detection method - Google Patents
Test paper, manufacturing method and detection method Download PDFInfo
- Publication number
- CN109612986A CN109612986A CN201811473785.9A CN201811473785A CN109612986A CN 109612986 A CN109612986 A CN 109612986A CN 201811473785 A CN201811473785 A CN 201811473785A CN 109612986 A CN109612986 A CN 109612986A
- Authority
- CN
- China
- Prior art keywords
- test paper
- enzyme
- manufacturing
- methylene blue
- fibrous membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of Test paper, manufacturing method and using the detection method of the test paper, the Test paper includes Hydrophilic Nanofibrous film；Enzyme, the enzyme are fixed on the Hydrophilic Nanofibrous film；The enzyme is suitable for that specific reaction occurs with test object；Methylene blue, the methylene blue adsorption number is in the hole of the Hydrophilic Nanofibrous film.The present invention has many advantages, such as that preparation is simple, detection sensitivity is high.
Description
Technical field
The present invention relates to the detections of phenolic compound, in particular to phenolic compound in Test paper, manufacturing method and water
Detection method.
Background technique
China's phenolic compounds pollution is extremely serious, promptly and accurately in determination of the environment the concentration of phenolic compound be prevention,
Control and basis and the premise for administering phenolic compounds pollution.
There is the detection method of phenols in national standard method:
1. spectrophotometry, complex compound formed in spectrophotometry is very unstable in aqueous solution, while examination used
Agent is organic solvent, there are problems that secondary recovery；
2. bromination volumetric method, sensitivity is low, is suitable only for the measurement of high concentration phenols wastewater；
3. chromatography；It is at high cost although high sensitivity, and need to pre-process the phenolic compound in water sample,
Process is cumbersome time-consuming.
In addition, environmental wastewater sample is complicated, when being detected using the above several method, there are the interference of coexisted organic compounds to ask
Topic.
Summary of the invention
In order to solve the deficiency in above-mentioned prior art, high, detection that the present invention provides a kind of detection sensitivities is fastly
Macroscopic Test paper that is fast, reusable, realizing phenolic compound detection.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of Test paper, the Test paper include Hydrophilic Nanofibrous film；The Test paper further comprises:
Enzyme, the enzyme are fixed on the Hydrophilic Nanofibrous film；The enzyme is suitable for that specificity occurs with test object
Reaction；
Methylene blue, the methylene blue adsorption number is in the hole of the Hydrophilic Nanofibrous film.
The object of the invention is also to provide the manufacturing method of above-mentioned Test paper, which is by following skill
Art scheme is achieved:
The manufacturing method of above-mentioned Test paper, the manufacturing method of the Test paper the following steps are included:
(A1) enzyme is fixed on Hydrophilic Nanofibrous film, the enzyme is suitable for that specific reaction occurs with test object；
(A2) by the Hydrophilic Nanofibrous film intrusion methylene blue solution for solidifying enzyme, methylene blue adsorption number is received described
In the hole of rice tunica fibrosa；
(A3) the free methylene blue on nano fibrous membrane is removed.
The object of the invention is also to provide the detection methods of phenolic compound in water, realize quick, accurate, naked eyes
Distinguishable detection, the goal of the invention have the technical scheme that
The detection method of phenolic compound in water, the detection method of phenolic compound in the water are as follows:
Above-mentioned detection is immersed in the water with test paper, it is anti-that catalysis oxidation occurs for the enzyme on the phenolic compound and test paper in water
It answers, generates benzoquinones；
Chromogenic reaction, the aobvious blue of methylene blue occur for the methylene blue on benzoquinones and test paper.
Compared with prior art, the device have the advantages that are as follows:
1. electrostatic spinning process is easy to operate, laccase and nano fibrous membrane raw material sources are extensive, low in cost, compensate for biography
Detection method of uniting disadvantage at high cost；
2. laccase overcomes in complex wastewater sample the catalytic oxidation of phenolic compound selectivity with higher
The interference that pollutant coexists eliminates the pretreatment process of sewage sample extracting and purifying；
3. the nanoscale of nano fibrous membrane makes it have, large specific surface area, porosity be high, the characteristic more than active site, can
With efficiently, fixing laccase in large quantities, hence it is evident that improve its detection sensitivity；
4. laccase is fixed on nano fibrous membrane by the process being chemically bonded, there is certain mechanical strength, be not easy to take off
It falls, makes it have reusing, the defect for overcoming the recycling of resolvase difficulty, being inactivated vulnerable to environmental influence；
5. the surface-crosslinked laccase of nano fibrous membrane makes nano fibrous membrane have high specific to targeted contaminants, overcomes
The low selectivity of conventional physical absorption；
6. exclusive color developing agent is added by the nano fibrous membrane in immobilization laccase, phenol generalization in naked eyes detection sewage is realized
Close object content.
Detailed description of the invention
Referring to attached drawing, the disclosure of the present invention will become more readily understood.Skilled addressee readily understands that: this
It is only used to illustrate the technical scheme of the present invention for a little attached drawings, and is not intended to limit the scope of protection of the present invention.
In figure:
Fig. 1 is the flow chart of the manufacturing method of according to embodiments of the present invention 1 Test paper.
Specific embodiment
Fig. 1 and following description describe optional embodiment of the invention with instruct those skilled in the art how to implement and
Reproduce the present invention.In order to teach the technical scheme of the invention, it has simplified or omitted some conventional aspects.Those skilled in the art answer
The variation or replacement that the understanding is originated from these embodiments will within the scope of the invention.Under those skilled in the art should understand that
Stating feature can combine in various ways to form multiple modifications of the invention.The invention is not limited to following optional as a result,
Embodiment, and be only limited by the claims and their equivalents.
Embodiment 1:
A kind of Test paper of the embodiment of the present invention, the Test paper include:
Hydrophilic Nanofibrous film；
Enzyme, the enzyme are fixed on the Hydrophilic Nanofibrous film；The enzyme is suitable for that specificity occurs with test object
Reaction；
Methylene blue, the methylene blue adsorption number is in the hole of the Hydrophilic Nanofibrous film.
In order to which enzyme to be fixed on the Hydrophilic Nanofibrous film, further, the enzyme is fixed on by cross-linking method
On the Hydrophilic Nanofibrous film.
In order to which the Test paper to be applied to the detection in phenolic compound, further, the enzyme is laccase, the inspection
Surveying object is phenolic compound.
Fig. 1 schematically illustrates the flow chart of the manufacturing method of the Test paper of the embodiment of the present invention, as shown in Figure 1,
The manufacturing method of above-mentioned Test paper the following steps are included:
(A1) enzyme is fixed on Hydrophilic Nanofibrous film, the enzyme is suitable for that specific reaction occurs with test object；
(A2) by the Hydrophilic Nanofibrous film intrusion methylene blue solution for solidifying enzyme, methylene blue adsorption number is received described
In the hole of rice tunica fibrosa；
(A3) the free methylene blue on nano fibrous membrane is removed.
For the ease of saving above-mentioned Test paper, further, the manufacturing method of the Test paper further comprise with
Lower step:
(A4) be freeze-dried nano fibrous membrane, 12~36h of drying time, temperature be -30 DEG C~-40 DEG C, pressure be 5~
15Pa。
In order to which enzyme is fixed on Hydrophilic Nanofibrous film, further, in step (A1), the curing mode of the enzyme
Are as follows:
The mass ratio of the glutaraldehyde of Hydrophilic Nanofibrous film and mass concentration 2%~10% is 3:1~10:1,20 DEG C~
Concussion reaction 10 at 30 DEG C~for 24 hours, nano fibrous membrane is activated；
It is later 3.00~5.00 with pH, the laccase solution that concentration is 0.2~1.5mg/mL mixes, and shakes at 20 DEG C~30 DEG C
Reaction 10~for 24 hours is swung, buffer solution rinses 3~5 times, freeze-drying.
In order to make methylene blue adsorption number in the hole of nano fibrous membrane, further, in step (A2), nano fibrous membrane
Mass ratio with leucomethylene blue is 3:1~10:1, and soaking time is 3~5min.
In order to obtain the Hydrophilic Nanofibrous film, further, the acquisition modes of Hydrophilic Nanofibrous film are as follows:
The quality proportioning of nano fibrous membrane and modifying agent is 1:0.5~1:3.5, and the modifying agent is sodium hydroxide and ethyl alcohol
Combination solution, quality proportioning are 1:20~1:50, and modified condition is 50 DEG C~90 DEG C of bath temperature, and the reaction time is 0.5~2h.
In order to obtain nano fibrous membrane at low cost, further, the acquisition modes of nano fibrous membrane are as follows:
Organosoluble polymer or molten polymer are made by electrostatic spinning technique under the electric field；
Polymer be polyvinyl alcohol, polyethylene glycol oxide, polyacrylic acid, polyacrylamide, polyacrylonitrile, in Kynoar
At least one, polymer solution concentration be 0.5~15wt%；
The requirement of the electrostatic spinning are as follows: voltage of electric field is 12~22kV, and syringe needle internal diameter is 0.40~2.00mm,
Boosting speed is 0.5~2.5mL/h, and spray distance is 5~15cm.
The detection method of phenolic compound in the water of the embodiment of the present invention, the detection method of phenolic compound in the water
Are as follows:
Above-mentioned detection is immersed in the water with test paper, it is anti-that catalysis oxidation occurs for the enzyme on the phenolic compound and test paper in water
It answers, generates benzoquinones；
Chromogenic reaction, the aobvious blue of methylene blue occur for the methylene blue on benzoquinones and test paper.
Embodiment 2:
Application examples of according to embodiments of the present invention 1 Test paper in sewage in phenolic compound detection.
In the application examples, the manufacturing method of Test paper the following steps are included:
(A0) nano fibrous membrane, acquisition modes are obtained are as follows:
Organosoluble polymer or molten polymer are made by electrostatic spinning technique under the electric field；
Polymer be polyvinyl alcohol, polyethylene glycol oxide, polyacrylic acid, polyacrylamide, polyacrylonitrile, in Kynoar
At least one, polymer solution concentration be 0.5~15wt%；
The requirement of the electrostatic spinning are as follows: voltage of electric field is 12~22kV, such as 12,18,22kV；Syringe needle internal diameter is
0.40~2.00mm, such as 0.40,1.50,2.00mm；Boosting speed is 0.5~2.5mL/h, such as 0.5,1.9,2.5mL/h；Injection
Distance is 5~15cm, such as 5,10,15cm；
The quality proportioning of nano fibrous membrane and modifying agent obtained above is 1:0.5~1:3.5, and the modifying agent is hydrogen-oxygen
Change sodium and ethyl alcohol combination solution, quality proportioning is 1:20~1:50, and modified condition is 50 DEG C~90 DEG C of bath temperature, the reaction time
For 0.5~2h, to obtain Hydrophilic Nanofibrous film；
(A1) laccase is fixed on Hydrophilic Nanofibrous film by cross-linking method, the enzyme is suitable for and phenol generalization in sewage
It closes object and catalytic oxidation occurs；The curing mode of the laccase are as follows:
The mass ratio of the glutaraldehyde of Hydrophilic Nanofibrous film and mass concentration 2%~10% is 3:1~10:1,20 DEG C~
Concussion reaction 10 at 30 DEG C~for 24 hours, nano fibrous membrane is activated；
It is later 3.00~5.00 with pH, the laccase solution that concentration is 0.2~1.5mg/mL mixes, and shakes at 20 DEG C~30 DEG C
Reaction 10~for 24 hours is swung, buffer solution rinses 3~5 times, freeze-drying；
(A2) the Hydrophilic Nanofibrous film for solidifying enzyme is invaded in methylene blue solution, nano fibrous membrane and leuco methylene
The mass ratio of base indigo plant is 3:1~10:1, and soaking time is 3~5min, hole of the methylene blue adsorption number in the nano fibrous membrane
In；
(A3) the free methylene blue on nano fibrous membrane is removed；
(A4) be freeze-dried nano fibrous membrane, 12~36h of drying time, temperature be -30 DEG C~-40 DEG C, pressure be 5~
15Pa。
The detection method of phenolic compound in the water of the embodiment of the present invention, the detection method of phenolic compound in the water
Are as follows:
The mathematical model for establishing leukomethylene colour developing degree and phenolic compound standard specimen concentration, obtains standard color comparison card；
Above-mentioned detection test paper is immersed in sewage, catalysis oxidation occurs for the enzyme on the phenolic compound and test paper in water
Reaction (laccase has efficient and single-minded catalysed oxidn to more than 40 kinds of phenolic compounds), generates quantitative and stable benzene
Quinone；
Exclusive chromogenic reaction, the aobvious blue of methylene blue occur for the leucomethylene blue on benzoquinones and test paper；
Test paper and standard color comparison card are compared, the concentration of phenolic compound in sewage sample can quickly be calculated.
Claims (10)
1. a kind of Test paper, the Test paper includes Hydrophilic Nanofibrous film；It is characterized by: the Test paper into
One step includes:
Enzyme, the enzyme are fixed on the Hydrophilic Nanofibrous film；The enzyme is suitable for that specific reaction occurs with test object；
Methylene blue, the methylene blue adsorption number is in the hole of the Hydrophilic Nanofibrous film.
2. Test paper according to claim 1, it is characterised in that: the enzyme is fixed on the hydrophily by cross-linking method
On nano fibrous membrane.
3. Test paper according to claim 2, it is characterised in that: the enzyme is laccase, and the test object is phenols
Compound.
4. the manufacturing method of -3 any Test papers according to claim 1, the manufacturing method of the Test paper include with
Lower step:
(A1) enzyme is fixed on Hydrophilic Nanofibrous film, the enzyme is suitable for that specific reaction occurs with test object；
(A2) by the Hydrophilic Nanofibrous film intrusion methylene blue solution for solidifying enzyme, methylene blue adsorption number is in the Nanowire
In the hole for tieing up film；
(A3) the free methylene blue on nano fibrous membrane is removed.
5. the manufacturing method of Test paper according to claim 4, it is characterised in that: the manufacturing method of the Test paper
It further includes steps of
(A4) it is freeze-dried nano fibrous membrane, 12~36h of drying time, temperature is -30 DEG C~-40 DEG C, and pressure is 5~15Pa.
6. the manufacturing method of Test paper according to claim 4, it is characterised in that: in step (A1), the enzyme is consolidated
Change mode are as follows:
The mass ratio of the glutaraldehyde of Hydrophilic Nanofibrous film and mass concentration 2%~10% is 3:1~10:1,20 DEG C~30 DEG C
Lower concussion reaction 10~for 24 hours, nano fibrous membrane is activated；
It is later 3.00~5.00 with pH, the laccase solution that concentration is 0.2~1.5mg/mL mixes, and shakes at 20 DEG C~30 DEG C anti-
Answer 10~for 24 hours, buffer solution rinses 3~5 times, freeze-drying.
7. the manufacturing method of Test paper according to claim 4, it is characterised in that: in step (A2), nano fibrous membrane
Mass ratio with leucomethylene blue is 3:1~10:1, and soaking time is 3~5min.
8. the manufacturing method of Test paper according to claim 4, it is characterised in that: the acquisition of Hydrophilic Nanofibrous film
Mode are as follows:
The quality proportioning of nano fibrous membrane and modifying agent is 1:0.5~1:3.5, and the modifying agent is that sodium hydroxide and ethyl alcohol compound
Solution, quality proportioning are 1:20~1:50, and modified condition is 50 DEG C~90 DEG C of bath temperature, and the reaction time is 0.5~2h.
9. the manufacturing method of Test paper according to claim 8, it is characterised in that: the acquisition modes of nano fibrous membrane
Are as follows:
Organosoluble polymer or molten polymer are made by electrostatic spinning technique under the electric field；
Polymer be polyvinyl alcohol, polyethylene glycol oxide, polyacrylic acid, polyacrylamide, polyacrylonitrile, in Kynoar extremely
Few one kind, polymer solution concentration are 0.5~15wt%；
The requirement of the electrostatic spinning are as follows: voltage of electric field is 12~22kV, and syringe needle internal diameter is 0.40~2.00mm, boosting
Speed is 0.5~2.5mL/h, and spray distance is 5~15cm.
10. the detection method of phenolic compound in water, the detection method of phenolic compound in the water are as follows:
Detection as claimed in any one of claims 1-3 is immersed in the water with test paper, the enzyme on phenolic compound and test paper in water
Catalytic oxidation occurs, generates benzoquinones；
Chromogenic reaction, the aobvious blue of methylene blue occur for the methylene blue on benzoquinones and test paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811473785.9A CN109612986A (en) | 2018-12-04 | 2018-12-04 | Test paper, manufacturing method and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811473785.9A CN109612986A (en) | 2018-12-04 | 2018-12-04 | Test paper, manufacturing method and detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109612986A true CN109612986A (en) | 2019-04-12 |
Family
ID=66006374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811473785.9A Pending CN109612986A (en) | 2018-12-04 | 2018-12-04 | Test paper, manufacturing method and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109612986A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112048828A (en) * | 2020-07-22 | 2020-12-08 | 西安交通大学 | Oil oxidation indicating membrane prepared by electrostatic spinning method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407803A (en) * | 2008-07-01 | 2009-04-15 | 北京师范大学 | Polylactic acid electrospinning fibre immobilized laccase and preparation thereof |
| CN101671665A (en) * | 2009-10-27 | 2010-03-17 | 北京师范大学 | Method for preparing immobilized laccase in electrospun fiber membrane |
| CN101915797A (en) * | 2010-07-27 | 2010-12-15 | 北京师范大学 | Method for preparing electrostatic spinning immobilized laccase electrode |
| CN103115919A (en) * | 2012-12-27 | 2013-05-22 | 中国科学院过程工程研究所 | Glucose detection test paper |
| CN108018281A (en) * | 2017-12-21 | 2018-05-11 | 华北电力大学 | A kind of application of immobilization laccase in methylene blue decoloration |
-
2018
- 2018-12-04 CN CN201811473785.9A patent/CN109612986A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407803A (en) * | 2008-07-01 | 2009-04-15 | 北京师范大学 | Polylactic acid electrospinning fibre immobilized laccase and preparation thereof |
| CN101671665A (en) * | 2009-10-27 | 2010-03-17 | 北京师范大学 | Method for preparing immobilized laccase in electrospun fiber membrane |
| CN101915797A (en) * | 2010-07-27 | 2010-12-15 | 北京师范大学 | Method for preparing electrostatic spinning immobilized laccase electrode |
| CN103115919A (en) * | 2012-12-27 | 2013-05-22 | 中国科学院过程工程研究所 | Glucose detection test paper |
| CN108018281A (en) * | 2017-12-21 | 2018-05-11 | 华北电力大学 | A kind of application of immobilization laccase in methylene blue decoloration |
Non-Patent Citations (1)
| Title |
|---|
| 袁海舰: "核桃楸树皮中活性物质的提取与分析", 《万方》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112048828A (en) * | 2020-07-22 | 2020-12-08 | 西安交通大学 | Oil oxidation indicating membrane prepared by electrostatic spinning method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| El-Moghazy et al. | Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk | |
| Wu et al. | Electrospun blend nanofiber membrane consisting of polyurethane, amidoxime polyarcylonitrile, and β-cyclodextrin as high-performance carrier/support for efficient and reusable immobilization of laccase | |
| Xu et al. | Enzymatic removal of paracetamol from aqueous phase: horseradish peroxidase immobilized on nanofibrous membranes | |
| CN101915797B (en) | Method for preparing electrostatic spinning immobilized laccase electrode | |
| Shimomura et al. | Amperometric detection of phenolic compounds with enzyme immobilized in mesoporous silica prepared by electrophoretic deposition | |
| Chao et al. | Immobilization of laccase onto porous polyvinyl alcohol/halloysite hybrid beads for dye removal | |
| Zheng et al. | Biosensor based on polyaniline-polyacrylonitrile-graphene hybrid assemblies for the determination of phenolic compounds in water samples | |
| CN107727717B (en) | Preparation method and application of polychlorinated biphenyl photoelectrochemical aptamer sensor | |
| Ren et al. | Recent environmental applications of and development prospects for immobilized laccase: a review | |
| CN106492879B (en) | Modified double scale polyacrylonitrile nanofiber iron complex catalyst of a kind of amidoxim and preparation method thereof | |
| Moradzadegan et al. | Immobilization of acetylcholinesterase in nanofibrous PVA/BSA membranes by electrospinning | |
| Zhao et al. | Multimeric immobilization of alcohol oxidase on electrospun fibers for valid tests of alcoholic saliva | |
| Li et al. | Electrospun nanofibers for enzyme immobilization | |
| Liu et al. | “Turn-on” fluorometric probe for hydroquinone and catechol based on an in situ reaction between protamine sulfate and dihydroxybenzene isomers and the formation of fluorescent polymer nanoparticles | |
| CN109612986A (en) | Test paper, manufacturing method and detection method | |
| CN108732218A (en) | A kind of electrochemical sensor measuring 2,4,6- trichlorophenol, 2,4,6,-Ts and its preparation and application | |
| Chen et al. | Kill two birds with one stone: Selective and fast removal and sensitive determination of oxytetracycline using surface molecularly imprinted polymer based on ionic liquid and ATRP polymerization | |
| Suresh et al. | Enzyme Immobilized nanomaterials: An electrochemical bio-sensing and biocatalytic degradation properties toward organic pollutants | |
| Yan et al. | Enzyme-containing hydrogel micropatterns serving a dual purpose of cell sequestration and metabolite detection | |
| Leca-Bouvier et al. | Polyluminol/hydrogel composites as new electrochemiluminescent-active sensing layers | |
| Lei et al. | A novel electrochemiluminescence glucose biosensor based on alcohol-free mesoporous molecular sieve silica modified electrode | |
| Suye et al. | Immobilization of glucose oxidase on poly‐(L‐lysine)‐modified polycarbonate membrane | |
| CN103913495B (en) | A kind of preparation method detecting the electrode of dopamine | |
| CN100339703C (en) | Biosensor electrode used for detecting aflatoxin and variegated aspergillin and its preparation method | |
| Oriero et al. | A potential enzyme-encapsulating, ultrafine fiber for phenol detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190412 |