CN109609574A - A kind of production technology of high concentration liquefying starch - Google Patents
A kind of production technology of high concentration liquefying starch Download PDFInfo
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- CN109609574A CN109609574A CN201811549902.5A CN201811549902A CN109609574A CN 109609574 A CN109609574 A CN 109609574A CN 201811549902 A CN201811549902 A CN 201811549902A CN 109609574 A CN109609574 A CN 109609574A
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- starch
- high concentration
- liquefying
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- amylase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
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Abstract
The invention discloses a kind of production technologies of high concentration liquefying starch, belong to starch processing technique field.The invention is made of following raw material and component: by weight, the ratio of water and starch is 1:1.2-1.6；By the investment meter of starch, wherein high temperature resistant а-amylase additive amount is 12-16U/ml(g), the additive amount of carbohydrase is 100-300 U/ml(g), and starch is made by high concentration liquefying starch by secondary liquefaction, primary saccharification, the high concentration liquefying starch is the mixture of glucose and oligosaccharide, is carbon source feed supplement raw material in a kind of good fermentation industry.Total fermented product yield not only can be improved using high concentration liquefying starch as feed supplement but also the concentration of fermented product in unit fermentation liquid can be improved, to be conducive to the fermentation process of fermented product upstream and the extraction process in downstream.Meanwhile compared with other carbon sources such as maltose, the high concentration liquefying starch advantage simple, low in cost with manufacture technology.
Description
Technical field
The present invention relates to a kind of starch deep process technology, a kind of specifically production technology of high concentration liquefying starch.
Background technique
In fermentation industry, in order to improve the yield of biomass and fermented product, it is often necessary to be filled into the fermentation middle and later periods
Nutrient, predominantly carbon source etc. in the raw material filled into, the general method using stream plus glucose.The problem is that needing to add
Entering certain moisture content liquid is made and sterilizes, after this usually appears in these raw materials of addition, total fermented product yield increases,
But the concentration for reducing fermented product in fermentation liquid, the extraction process for continuing product after being cause to bear.
Based on the above reasons, the liquefying starch for needing a kind of high concentration, the starch after liquefaction are glucose and oligosaccharide
Mixture, be a kind of good carbon source feed supplement raw material, total fermented product not only can be improved in this high concentration liquefying starch
Yield, and the concentration of fermented product can be improved.
The present invention is by using starch, water as raw material, using high temperature resistant а-amylase and carbohydrase as biological enzyme agent, into
Row secondary batching, secondary temperature elevation liquefaction, cooling, saccharification and etc., high concentration liquefying starch is made in starch, utilizes high concentration liquid
Changing starch not only can be improved total fermented product yield as benefit amount but also fermented product in unit fermentation liquid can be improved
Concentration, to be conducive to the fermentation process of fermented product upstream and the extraction process in downstream.Meanwhile with other maltoses, dextrin etc.
Carbon source is compared, the advantage that high concentration liquefying starch has manufacture technology simple, low in cost.
Summary of the invention
The main purpose of the present invention is to provide a kind of production technologies of high concentration liquefying starch, it is characterised in that this is highly concentrated
Liquefying starch is spent using starch, water as raw material, using high temperature resistant а-amylase and carbohydrase as biological enzyme agent；Proportion is with weight
Meter, the ratio of water and starch are 1:1.2-1.6, the additive amount of high temperature resistant а-amylase and carbohydrase with the investment meter of starch,
Wherein high temperature resistant а-amylase additive amount is 12-16U/g, and the additive amount of carbohydrase is 100--300 U/g.
A kind of production technology of high concentration liquefying starch, it is characterised in that (1) feed intake liquefaction for the first time, by water and starch
Ratio is 1:1-1.2, and whole ingredient waters is first added, and opens liquefied pot stirring, opens liquefied pot steam heating valve, by 40-
In 50% starch step by step input liquefied pot, when temperature is raised to 60-70 DEG C, meter is thrown by total, the high temperature resistant а-of 6-8 U/g is added
Amylase, when temperature rises 90-95 DEG C, the process of feeding intake terminates for the first time, steam off valve, at 90-95 DEG C of maintenance 10-20 points
Clock, first time liquefaction process terminate.(2) cooling water valve is opened, the temperature of liquefied starch is controlled at 55-60 DEG C, is closed
The carbohydrase of 100--300U/g is added based on the total amount of feeding of starch in cooling water valve, maintains 45-60 minutes.
(3) feed intake liquefaction for the second time, opens steam valve, in remaining starch investment liquefied pot, stirs when feeding intake
Side heating, when temperature rises to 90-95 DEG C, and the resistance to of 6-8 U/g is added based on the total amount of feeding of starch in steam off valve
High temperature а-amylase, liquefy 10-20 clock, closes agitating device.
A kind of production technology of high concentration liquefying starch, it is characterised in that by the high concentration when high concentration liquefying starch uses
Liquefying starch is pumped into after feed supplement tank high-temperature sterilization as fermentor feed supplement.
Benefit of the invention:
1, total fermented product yield not only can be improved in high concentration liquefying starch, but also the concentration of fermented product can be improved;
2, compared with making feed supplement with maltose, manufacturing process is simple, low in cost.
Specific embodiment
Embodiment one
The ratio of a kind of production technology of high concentration liquefying starch, raw material preparation, water and starch is 1:1.2, i.e. 6 tons of starch, is matched
5 tons of water of material, liquid high temperature resistant а-amylase 360 ml, 100000U/m 6000 ml of liquid saccharifying enzyme of 20000U/ml is pressed
Following steps operation:
(1) feed intake liquefaction for the first time, and 5 tons of ingredient waters are first added, and opens liquefied pot stirring, opens liquefied pot steam heating valve,
By in 3 tons of starch step by step input liquefied pot, when temperature is raised to 60-70 DEG C, high temperature resistant а-amylase of 180ml is added, when
Temperature rises 90-95 DEG C, and the process of feeding intake terminates for the first time, steam off valve, maintains 10 minutes at 90-95 DEG C, liquefies for the first time
Process terminates;
(2) cooling water valve is opened, the temperature of liquefied starch is controlled at 55-60 DEG C, cooling water valve is closed, by always feeding intake
The carbohydrase of 6000ml is added in meter, maintains 45-60 minutes;
(3) feed intake liquefaction for the second time, opens steam valve, remaining 3 tons of starch is put into liquefied pot, is stirred when feeding intake
Side heating, when temperature rises to 90-95 DEG C, and steam off valve, high temperature resistant а-amylase of 180ml is added, liquefy 10 clocks
, agitating device is closed, the high concentration liquefying starch is pumped into after feed supplement tank high-temperature sterilization as fermentor feed supplement when using.
Embodiment two
The ratio of a kind of production technology of high concentration liquefying starch, raw material preparation, water and starch is 1:1.5, i.e. 7.5 tons of starch,
5 tons of ingredient water, liquid high temperature resistant а-amylase 500ml, 100000U/m 15000 ml of liquid saccharifying enzyme of 20000U/ml,
It operates according to the following steps:
(1) feed intake liquefaction for the first time, and 5 tons of ingredient waters are first added, and opens liquefied pot stirring, opens liquefied pot steam heating valve,
By in 3 tons of starch step by step input liquefied pot, when temperature is raised to 60-70 DEG C, high temperature resistant а-amylase of 250ml is added, when
Temperature rises 90-95 DEG C, and the process of feeding intake terminates for the first time, steam off valve, maintains 15 minutes at 90-95 DEG C, liquefies for the first time
Process terminates;
(2) cooling water valve is opened, the temperature of liquefied starch is controlled at 55-60 DEG C, cooling water valve is closed, is added
The carbohydrase of 15000ml maintains 50 minutes;
(3) feed intake liquefaction for the second time, opens steam valve, in remaining 3.5 tons of starch investment liquefied pot, stirs when feeding intake
Side heating, when temperature rises to 90-95 DEG C, and high temperature resistant а-amylase of 250ml is added in steam off valve, and 15 clocks of liquefaction are
Can, agitating device is closed, the high concentration liquefying starch is pumped into after feed supplement tank high-temperature sterilization as fermentor feed supplement when using.
Embodiment three
The ratio of a kind of production technology of high concentration liquefying starch, raw material preparation, water and starch is 1:1.6, i.e. 8 tons of starch, is matched
5 tons of water of material, liquid high temperature resistant а-amylase 640ml, 100000U/m 24000 ml of liquid saccharifying enzyme of 20000U/ml is pressed
Following steps operation:
(1) feed intake liquefaction for the first time, and 5 tons of ingredient waters are first added, and opens liquefied pot stirring, opens liquefied pot steam heating valve,
By in 4 tons of starch step by step input liquefied pot, when temperature is raised to 60-70 DEG C, high temperature resistant а-amylase of 320ml is added, when
Temperature rises 90-95 DEG C, and the process of feeding intake terminates for the first time, steam off valve, maintains 20 minutes at 90-95 DEG C, liquefies for the first time
Process terminates;
(2) cooling water valve is opened, the temperature of liquefied starch is controlled at 55-60 DEG C, cooling water valve is closed, is added
The carbohydrase of 24000ml maintains 60 minutes;
(3) feed intake liquefaction for the second time, opens steam valve, remaining 4 tons of starch is put into liquefied pot, while feeding intake while stirring
Heating, when temperature rises to 90-95 DEG C, and high temperature resistant а-amylase of 320ml is added in steam off valve, and 20 clocks of liquefaction are
Can, agitating device is closed, the concentration liquefying starch is pumped into after feed supplement tank high-temperature sterilization as fermentor feed supplement when using.
Above are only presently preferred embodiments of the present invention, it is not intended to restrict the invention, it is all in spirit of the invention
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (3)
1. a kind of production technology of high concentration liquefying starch, it is characterised in that the high concentration liquefying starch is main with starch, water
Raw material, using high temperature resistant а-amylase and carbohydrase as biological enzyme agent；By weight, the ratio of water and starch is 1 to proportion:
The additive amount of 1.2-1.6, high temperature resistant а-amylase and carbohydrase is with the investment meter of starch, and wherein high temperature resistant а-amylase adds
Dosage is 12-16U/ g, and the additive amount of carbohydrase is 100--300 U/ g.
2. a kind of production technology of high concentration liquefying starch, it is characterised in that (1) feed intake liquefaction for the first time, by the ratio of water and starch
Example is 1:1-1.2, whole ingredient waters is first added, the stirring of unlatching liquefied pot, opening liquefied pot steam heat valve, by 40-50%
In starch step by step input liquefied pot, when temperature is raised to 60-70 DEG C, by the meter that feeds intake of total starch, the resistance to of 6-8 U/ g is added
High temperature а-amylase, when temperature rises 90-95 DEG C, the process of feeding intake terminates, steam off valve, at 90-95 DEG C of maintenance 10-20 points
Clock, first time liquefaction process terminate;
(2) cooling water valve is opened, the temperature of liquefied starch is controlled at 55-60 DEG C, cooling water valve is closed, by total shallow lake
Powder feeds intake meter, and the carbohydrase of 100--300 U/g is added, and maintains 45-60 minutes;
(3) feed intake liquefaction for the second time, opens steam valve, in remaining starch investment liquefied pot, rises while stirring while feeding intake
Temperature, when temperature rises to 90-95 DEG C, and the high temperature resistant of 6-8 U/g is added based on the total amount of feeding of starch in steam off valve
а-amylase, liquefy 10-20 clock.
3. a kind of production technology of high concentration liquefying starch as claimed in claim 1 or 2, it is characterised in that high concentration liquefaction is formed sediment
The high concentration liquefying starch is pumped into after feed supplement tank high-temperature sterilization as fermentor feed supplement when powder uses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201811549902.5A CN109609574A (en) | 2018-12-18 | 2018-12-18 | A kind of production technology of high concentration liquefying starch |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811549902.5A CN109609574A (en) | 2018-12-18 | 2018-12-18 | A kind of production technology of high concentration liquefying starch |
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| CN109609574A true CN109609574A (en) | 2019-04-12 |
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| CN201811549902.5A Pending CN109609574A (en) | 2018-12-18 | 2018-12-18 | A kind of production technology of high concentration liquefying starch |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111252897A (en) * | 2020-02-15 | 2020-06-09 | 北京环球中科水务科技有限公司 | Carbonyl composite carbon source biological carbon supplement agent and preparation method thereof |
-
2018
- 2018-12-18 CN CN201811549902.5A patent/CN109609574A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111252897A (en) * | 2020-02-15 | 2020-06-09 | 北京环球中科水务科技有限公司 | Carbonyl composite carbon source biological carbon supplement agent and preparation method thereof |
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Application publication date: 20190412 |