CN109609572B - Preparation method of mannan with specific molecular weight - Google Patents
Preparation method of mannan with specific molecular weight Download PDFInfo
- Publication number
- CN109609572B CN109609572B CN201910009954.1A CN201910009954A CN109609572B CN 109609572 B CN109609572 B CN 109609572B CN 201910009954 A CN201910009954 A CN 201910009954A CN 109609572 B CN109609572 B CN 109609572B
- Authority
- CN
- China
- Prior art keywords
- enzymolysis
- molecular weight
- mannan
- digital display
- container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/16—Screw conveyor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing mannan with specific molecular weight by enzymolysis of hemicellulose, which mainly adopts a special enzymolysis system for controllable enzymolysis, wherein the enzymolysis system comprises an enzymolysis container, a double-screw propulsion device, a digital display temperature control system, a molecular weight online detection digital display device and a gear pump. The enzymolysis system can be used for accurately controlling the enzymolysis degree to obtain mannan with required molecular weight or molecular weight in different sections; the enzymolysis system solves the problems of high viscosity and easy caking during feeding in the enzymolysis process of hemicellulose, improves the enzymolysis rate, and shortens the enzymolysis process by one third to one half compared with an unmodified enzymolysis device under the same condition; meanwhile, enzymolysis and enzyme deactivation can be carried out in the same container, external enzyme deactivation equipment is not needed, the production flow is simplified, and efficiency and energy are improved.
Description
Technical Field
The invention belongs to the field of biochemical engineering, and particularly relates to a device and a method for preparing mannan with a specific molecular weight.
Background
Mannans are linear polysaccharides linked by β -1, 4-D-mannopyranoside linkages, and if some of the residues in the backbone are replaced by glucose or galactose is linked to mannose residues via 1,6- α -glycosidic linkages to form branches, they are called isomannins, mainly galactomannans, glucomannans and galactoglucomannans. Because the types, the polymerization degrees, the sugar bond connections, the arrangement modes and the like of monosaccharides forming the polysaccharides are different, the solubility, the viscosity and other properties of different polysaccharides are obviously different, and the hydrolysis of the beta-mannase is greatly influenced. Thus limiting the use of mannan to some extent.
In the research on catalytic degradation of mannan enzymes, most of researches focus on degrading mannan into oligosaccharide with very low molecular weight, wherein the low-molecular-weight mannose is a kind of sugar with low polymerization degree, the polymerization degree of sugar molecules is 2-10, and the low-molecular-weight mannose is widely applied to the fields of food, chemical industry, medicine and the like. The studies on other molecular weight blocks of mannan are few, and mainly relate to the preparation patents of mannose (patent 201711340163.4), mannan (201711340162. X) and mannan (201711341567.5), the molecular weight blocks of mannan have different functional characteristics and are respectively applied to different fields, but the molecular weight range of mannan (400Da-120 ten thousand Da) is wide, and many other molecular weight blocks of mannan wait for preparation and excavation of functional characteristics.
At present, researchers prepare mannans with different molecular weights, generally control the molecular weight of products through enzymolysis time and enzyme addition amount, and have the disadvantages of high randomness, low reproducibility and incomplete molecular weight of prepared products. Some methods control enzymolysis by controlling molecular weight, for example, gel permeation chromatography-laser light scattering combined technology, nuclear magnetic resonance spectroscopy, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and other technologies can be adopted to determine the molecular weight of the enzymolysis solution, and the accuracy of the measurement result is good, but real-time online detection cannot be realized due to complex detection procedures and long time consumption. Although the viscosity method is an indirect detection method for molecular weight, the method has the advantages of simple instrument and equipment, convenience in operation, wide application range of molecular weight and similar detection accuracy, can detect the viscosity on line in real time, and obtains the molecular weight through a relational expression of the viscosity and the molecular weight.
The invention aims to solve the problem that a molecular weight online detection device is arranged on an enzymolysis container, and mannan with any required molecular weight or molecular weight range is obtained by detecting the molecular weight on line and accurately controlling the enzymolysis degree.
When mannan of different molecular weight district section is prepared in the enzymolysis, because raw materials granule is tiny, caking very easily when throwing the material, especially throw the material and carry out half the time, the enzymolysis liquid is very sticky, when continuing to spill the material, some tiny material granules can be wrapped up inside, and can not carry out the enzymolysis fully. In the prior art, a method for solving the problem of avoiding caking during feeding of konjak, guar gum, locust bean gum and the like mainly relates to the steps of reducing the concentration of a substrate, stirring, adding ions or pretreating by using certain reagents. Caking can be avoided by reducing the substrate concentration, but is inefficient for large-scale production; the stirring is applied to the large-scale production, the efficiency is low, and the caking phenomenon still occurs because of the uneven stirring; ions are added or certain reagents are used for treatment, so that the ions are easy to remain in degradation products, the purification difficulty is increased, and the application of the ions in the fields of food and medicine is limited.
The invention adopts a special enzymolysis system for controllable enzymolysis, the enzymolysis system comprises an enzymolysis container, a double-screw propulsion device, a digital display temperature control system, a molecular weight online detection digital display device and a gear pump, the problems of high viscosity and feeding caking can be solved, and the enzymolysis speed is improved; meanwhile, enzymolysis and enzyme deactivation can be carried out in the same enzymolysis container, external enzyme deactivation equipment is not needed, the production flow is simplified, and efficiency and energy are improved.
Disclosure of Invention
The invention discloses a production method of mannan with specific molecular weight, which mainly adopts a special enzymolysis system to carry out controllable enzymolysis, wherein the enzymolysis system comprises an enzymolysis container, a double-screw propulsion device, a digital display temperature control system, a molecular weight online detection digital display device and a gear pump. The enzymolysis system can be used for accurately controlling the enzymolysis degree to obtain the mannan with the required molecular weight or molecular weight in different sections.
The feed liquid is pushed by the double-screw pushing device and the gear pump, the enzymolysis is circularly carried out, the problems of high viscosity and easy caking during feeding in the hemicellulose enzymolysis process can be solved, the enzymolysis rate is improved, and the enzymolysis process is shortened by one third to one half compared with the original enzymolysis process under the same condition; the lower outlet position of the enzymolysis container is provided with a molecular weight online detection digital display device, and when the target molecular weight is detected to be small, the digital display temperature control system can automatically start the heating function of the double-screw propulsion device to heat and inactivate enzyme according to a set program; the temperature range of the digital display temperature control system is 0-150 ℃. When the substrate material liquid is subjected to enzymolysis, the temperature is automatically regulated to 30-35 ℃, when the enzyme is deactivated, the temperature is automatically regulated to 100-105 ℃, the enzymolysis and the enzyme deactivation can be carried out in the same container, external enzyme deactivation equipment is not needed, the production flow is simplified, and the effects and the energy are increased.
In order to obtain the technical effects, the technical scheme adopted by the invention is as follows:
mannan with mannan-containing hemicellulose such as konjac gum, guar gum, locust bean gum, sesbania gum and the like is used as a raw material, a special enzymolysis system is adopted for controllable enzymolysis, and mannan with required molecular weight or molecular weight intervals is finally obtained through a series of procedures such as centrifugation, membrane separation, ion exchange and the like.
The preparation process of mannan with specific molecular weight is as follows:
hemicellulose → controllable enzymolysis → centrifugal separation → membrane separation → ion exchange → spray drying → obtain mannan with specific molecular weight
In the process of preparing mannan with specific molecular weight, the controllable enzymolysis process is the most critical, the invention carries out creative integrated design on the existing enzymolysis tank and enzyme deactivation device, and establishes a special enzymolysis system which comprises an enzymolysis container 1, a double-screw propulsion device 2, a digital display temperature control system 3, a molecular weight online detection digital display device 4 and a gear pump 5;
the temperature range of a digital temperature control system in the enzymolysis system is 0-150 ℃, the temperature is automatically regulated to 30-35 ℃ during substrate material liquid enzymolysis, and the temperature is automatically regulated to 100-105 ℃ during enzyme deactivation;
the molecular weight online detection digital display device 4 in the enzymolysis system consists of a sensor probe and a transmitter, wherein the frequency of the sensor vibration probe is 50-80Hz, and the amplitude is 1-2 mm; the constant amplitude is stabilized by current according to the design of torque oscillation principle. When the probe sensor is in work, after the probe sensor is completely immersed in the enzymatic hydrolysate, the enzymatic hydrolysates with different enzymolysis degrees have different viscosities, so that phase changes with different amplitudes are generated between the enzymatic hydrolysate and the surface of the sensor probe, the sensor probe is a micro-amplitude resonance shear wave which is always kept the same, the change of the fluid viscosity is reflected by a current signal which keeps the sensor probe under a fixed amplitude, the current signal which is changed is converted into the viscosity value of the enzymatic hydrolysate by the transmitter, and then the viscosity value is converted into the molecular value according to a relational expression of the viscosity value and the molecular weight in a program.
Specifically, in the process of enzymolysis of mannan, the molecular weight is detected on line, the viscosity of enzymolysis liquid is reduced along with the enzymolysis of mannan, the current for maintaining the sensor probe under a fixed amplitude is reduced, and the molecular weight is converted into the molecular weight of mannan through a program and is displayed on a digital display device.
A feeding port 6 for adding raw materials and enzymes and a discharge port 7 for discharging enzymolysis liquid are arranged on the enzymolysis container 1;
in a further technical scheme, the obtained mannan with the specific molecular weight can be a single molecular weight component or a certain molecular weight interval component.
Compared with the prior art, the integrated design of the enzymolysis and enzyme deactivation process has the following advantages:
(1) the required enzymolysis node is judged through the molecular weight online detection digital display device, when the target molecular weight is detected, the digital display temperature control system can automatically start the heating function of the double-screw propulsion device to heat rapidly according to a set program, enzyme is deactivated in time, and the enzymolysis product in the required molecular weight range can be accurately and conveniently obtained in industrial production.
(2) The enzymolysis system is equipped with double screw advancing device and gear pump, and the feed liquid is under their common promotion, and the enzymolysis circulation goes on, can solve the hemicellulose enzymolysis in-process viscosity height to and easily the caking problem when throwing the material, improve enzymolysis rate, the enzymolysis process shortens one third to half the time.
(3) Enzymolysis and enzyme deactivation are carried out in the same container device, external enzyme deactivation equipment is not needed, the production flow is simplified, and efficiency and energy are improved.
Drawings
FIG. 1 shows a special enzymolysis system device of the present invention.
Detailed Description
Example 1
Injecting 450kg of production water into an enzymolysis container, adjusting the temperature in the enzymolysis container to be 30 ℃, adjusting the pH value to be 6.0-7.0, adding neutral beta-mannase (the adding amount of the enzyme is calculated according to the mass ratio of enzyme to material being 1: 15) into the enzymolysis container, then weighing 50kg of konjac powder, adding into the enzymolysis container, carrying out enzymolysis circulation under the push of a double-screw propulsion device and a gear pump, adjusting the frequency of a vibration probe of a molecular weight online detection digital display device to be 80Hz and the amplitude to be 1mm, gradually reducing the viscosity of an enzymolysis liquid along with the enzymolysis, when the molecular weight of the enzymolysis liquid detected by the molecular weight online detection digital display device is 100000-, cooling; then a series of procedures such as centrifugation, membrane separation, ion exchange and the like are carried out, and finally the mannan with the molecular weight of 100000-200000Da is obtained.
Example 2
Injecting 450kg of production water into an enzymolysis container, adjusting the temperature in the enzymolysis container to be 30 ℃ and the pH value to be 6.0-7.0, adding neutral beta-mannase (the adding amount of the enzyme is calculated according to the mass ratio of enzyme to material being 1: 15) into the enzymolysis container, then weighing 50kg of guar gum, adding into the enzymolysis container, performing circular enzymolysis under the push of a double-screw propulsion device and a gear pump, adjusting the frequency of a vibration probe of a molecular weight online detection digital display device to be 80Hz and the amplitude to be 1mm, gradually reducing the viscosity of an enzymolysis liquid along with the enzymolysis, when the molecular weight online detection digital display device detects that the molecular weight of the enzymolysis liquid is 10000-, cooling; then a series of procedures such as centrifugation, membrane separation, ion exchange and the like are carried out to finally obtain the mannan with the molecular weight of 10000-12000 Da.
Example 3
Injecting 450kg of production water into an enzymolysis container, adjusting the temperature in the enzymolysis container to be 30 ℃, adjusting the pH value to be 6.0-7.0, adding neutral beta-mannase (the adding amount of the enzyme is calculated according to the mass ratio of enzyme to material being 1: 15) into the enzymolysis container, then weighing 50kg of locust bean gum, adding into the enzymolysis container, performing circular enzymolysis under the push of a double-screw propulsion device and a gear pump, adjusting the frequency of a vibration probe of a molecular weight online detection digital display device to be 50Hz and the amplitude to be 2mm, gradually reducing the viscosity of an enzymolysis liquid along with the enzymolysis, when the molecular weight online detection digital display device detects that the molecular weight of the enzymolysis liquid is 3000-, cooling; then a series of procedures such as centrifugation, membrane separation, ion exchange and the like are carried out, and finally the mannan with the molecular weight of 3000-.
The above description is not intended to limit the present invention, and the present invention is not limited to the above examples. Those skilled in the art should also realize that changes, modifications, additions and substitutions can be made without departing from the true spirit and scope of the invention.
Claims (1)
1. A preparation method of mannan with specific molecular weight is characterized in that mannan-containing hemicellulose of konjac gum, guar gum, locust bean gum and sesbania gum is used as raw materials, and a special enzymolysis system is adopted for controllable enzymolysis;
the special enzymolysis system is characterized in that an enzymolysis tank and an enzyme deactivation device are integrated and designed;
the special enzymolysis system comprises an enzymolysis container 1, a double-screw propulsion device 2, a digital display temperature control system 3, a molecular weight online detection digital display device 4 and a gear pump 5;
the double-screw propulsion device 2 in the enzymolysis system contains a rapid heating function, and the heating function is controlled by a program of the digital display temperature control system 3 to accurately inactivate enzyme;
the temperature range of a digital temperature control system 3 in the enzymolysis system is 0-150 ℃, the temperature is automatically regulated to 30-35 ℃ when the substrate material liquid is subjected to enzymolysis, and the temperature is automatically regulated to 100-105 ℃ when the enzyme is inactivated;
the frequency of a sensor vibration probe of the molecular weight online detection digital display device 4 in the enzymolysis system is 50-80Hz, and the amplitude is 1-2 mm;
the mannans with specific molecular weights obtained by the enzymolysis system are respectively the mannans with molecular weights of 100000-200000Da, 10000-12000Da and 3000-5000 Da.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910009954.1A CN109609572B (en) | 2019-01-06 | 2019-01-06 | Preparation method of mannan with specific molecular weight |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910009954.1A CN109609572B (en) | 2019-01-06 | 2019-01-06 | Preparation method of mannan with specific molecular weight |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109609572A CN109609572A (en) | 2019-04-12 |
| CN109609572B true CN109609572B (en) | 2022-06-21 |
Family
ID=66016787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910009954.1A Active CN109609572B (en) | 2019-01-06 | 2019-01-06 | Preparation method of mannan with specific molecular weight |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109609572B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203613198U (en) * | 2013-11-18 | 2014-05-28 | 中国农业科学院农产品加工研究所 | Reaction system for preparing high molecular weight vegetable protein peptide |
| CN105567761A (en) * | 2014-10-16 | 2016-05-11 | 中粮营养健康研究院有限公司 | Enzymolysis method for cellulose-contained material |
| CN106755201A (en) * | 2017-03-23 | 2017-05-31 | 中国海洋大学 | A kind of preparation method of galactomannan oligosaccharide |
| CN107760740A (en) * | 2017-12-14 | 2018-03-06 | 宁波拜尔玛生物科技有限公司 | A kind of big sugar of high-purity kanjak mannan and preparation method thereof, application |
| CN207091430U (en) * | 2017-08-18 | 2018-03-13 | 成都长力元生物科技有限公司 | A kind of equipment of efficiently enzymolysis glucomannan |
| CN107982275A (en) * | 2017-12-14 | 2018-05-04 | 宁波拜尔玛生物科技有限公司 | Application of the big sugar of sweet dew in gynaecologic antibiotic product |
-
2019
- 2019-01-06 CN CN201910009954.1A patent/CN109609572B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203613198U (en) * | 2013-11-18 | 2014-05-28 | 中国农业科学院农产品加工研究所 | Reaction system for preparing high molecular weight vegetable protein peptide |
| CN105567761A (en) * | 2014-10-16 | 2016-05-11 | 中粮营养健康研究院有限公司 | Enzymolysis method for cellulose-contained material |
| CN106755201A (en) * | 2017-03-23 | 2017-05-31 | 中国海洋大学 | A kind of preparation method of galactomannan oligosaccharide |
| CN207091430U (en) * | 2017-08-18 | 2018-03-13 | 成都长力元生物科技有限公司 | A kind of equipment of efficiently enzymolysis glucomannan |
| CN107760740A (en) * | 2017-12-14 | 2018-03-06 | 宁波拜尔玛生物科技有限公司 | A kind of big sugar of high-purity kanjak mannan and preparation method thereof, application |
| CN107982275A (en) * | 2017-12-14 | 2018-05-04 | 宁波拜尔玛生物科技有限公司 | Application of the big sugar of sweet dew in gynaecologic antibiotic product |
Non-Patent Citations (3)
| Title |
|---|
| Enzymatic Degradation of Guar and Substituted Guar Galactomannans;Yu Cheng等;《Biomacromolecules》;20001103;第1卷(第4期);第782–788页 * |
| 纤维素酶制备魔芋葡甘低聚糖;张迎庆等;《吉首大学学报(自然科学版)》;20030925;第24卷(第03期);第42-44页 * |
| 酶催化魔芋葡甘聚糖的可控降解;祁黎等;《高分子学报》;20030920(第5期);摘要、实验方法 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109609572A (en) | 2019-04-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xie et al. | Effects of high hydrostatic pressure and high pressure homogenization processing on characteristics of potato peel waste pectin | |
| Freitas et al. | Advances in bacterial exopolysaccharides: from production to biotechnological applications | |
| Charles | Technical aspects of the rheological properties of microbial cultures | |
| Onumpai et al. | Microbial utilization and selectivity of pectin fractions with various structures | |
| Hwang et al. | Controlling molecular weight and degree of deacetylation of chitosan by response surface methodology | |
| Pu et al. | Molecular weight kinetics and chain scission models for dextran polymers during ultrasonic degradation | |
| KR101417698B1 (en) | Method and apparatus for mixing a lignocellulosic material with enzymes | |
| CN105504095B (en) | A kind of method for preparing chitosan oligomer using ultrasonic waterpower collaboration cavitation | |
| JP2013539658A (en) | High concentration solid enzyme reactor or mixer and method | |
| CN102617751A (en) | Method for preparing pectin oligosaccharide (POS) through dynamic high-pressure microfluidization (DHPM) combined acid process | |
| CN109609572B (en) | Preparation method of mannan with specific molecular weight | |
| JP2005110675A (en) | Method for producing low-molecular polysaccharide and/or oligosaccharide, and functional low-molecular polysaccharide and/or oligosaccharide | |
| CN101475969B (en) | Method for degrading chitosan by using papain | |
| US20220112314A1 (en) | Ultrasound and pressure assisted method for extracting pectin rich in rg-i | |
| CN103436572A (en) | Preparation method of tubular starch derivative | |
| Hu et al. | Effect of solution pulsed plasma process on the degradation and physicochemical properties of pectin | |
| CN105154496B (en) | A method of specified molecular weight water soluble chitosan is prepared using enzyme process | |
| Wheatley et al. | Degradation of polysaccharides by endo‐and exoenzymes: Dextran–dextranase model systems | |
| US3706598A (en) | Solid,glass-like starch hydrolysates having high densities | |
| CN103833868A (en) | Rapid preparation method for megalosaccharide | |
| Lee et al. | Lipase-catalyzed regioselective synthesis of dextrin esters | |
| CN205473511U (en) | Supersound - water conservancy of preparation chitosan oligosaccharides is in coordination with cavitation device | |
| Ming et al. | Production of chitosan oligosaccharides at high concentration by immobilized chitosanase | |
| CN103030712A (en) | Method for preparing konjac oligo-glucomannan | |
| Tavernier et al. | β-(1, 4)-polyglucuronic acids–An overview |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220512 Address after: 443000 No. 6-112, Longzhong back road, Wujiagang District, Yichang City, Hubei Province Applicant after: Zhao Qinnong Address before: 313000 room 601-608, building 008, No. 11, 12 and 15, Lane 299, Guanghua Road, Ningbo hi tech Zone, Ningbo, Zhejiang Province Applicant before: NINGBO BIOMART BIOTECHNOLOGY CO.,LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |