CN109603951B

CN109603951B - Pipe frame

Info

Publication number: CN109603951B
Application number: CN201910031077.8A
Authority: CN
Inventors: 陈继欣
Original assignee: Wise Thinking Beijing Technology Co ltd
Current assignee: Wise Thinking Beijing Technology Co ltd
Priority date: 2017-09-21
Filing date: 2017-09-21
Publication date: 2021-05-18
Anticipated expiration: 2037-09-21
Also published as: CN107744839B; CN109603951A; CN107744839A

Abstract

The application provides a pipe support, including first pipe support unit. The first pipe rack unit includes a plurality of first function holes and a plurality of second function holes. The number of the first functional holes is the same as that of the second functional holes. The first functional holes and the second functional holes are alternately arranged along the first direction, and any two first functional holes or any two second functional holes are not adjacent along the first direction. The first functional holes and the second functional holes are alternately arranged along the second direction, and any two first functional holes or any two second functional holes are not adjacent along the second direction. This application utilizes the cooperation in first function hole and second function hole through setting up a plurality of first function holes and a plurality of second function hole, can make the liquid of placing in the downthehole collector tube of first function can the quick removal of high flux get to the work efficiency who moves the liquid work station has been increased.

Description

Pipe frame

RELATED APPLICATIONS

This application is a divisional application of the chinese patent application entitled "a pipe rack" filed on 2017, 9, 21 and application No. 201710860060.4, which is incorporated herein by reference in its entirety.

Technical Field

The application relates to the technical field of equipment, in particular to a pipe support.

Background

In recent years, with the increasing demand for high throughput pipetting operations, pipetting stations are used in large quantities in the fields of nucleic acid extraction, drug development, and the like. Laboratory sample processing speed and efficiency can be greatly improved through the liquid transfer workstation.

However, one of the bottlenecks is that most of the single liquid collecting pipes are large in size and cannot be placed on a 96-well plate. Therefore, the liquid is moved into the micro-porous plate from a single liquid collecting tube by one or one row of operation, the high-flux quick moving is not realized, and the working efficiency of the liquid moving workstation is greatly clamped.

Therefore, it becomes especially urgent to rapidly transfer liquid samples in different collection tubes into a microplate for high throughput operations.

Disclosure of Invention

Based on this, it is necessary to provide a pipe support to current collecting tube can't realize that high flux moves fast and gets, leads to pipetting workstation's work efficiency greatly reduced's problem.

The technical problem that this application mainly solved is mainly realized by following structure: this application divide into two pipe support units, first pipe support unit and second pipe support unit. Each tube frame unit size conforms to the microplate ANSI/SBS1-2004 standard, can be placed on a pipetting workstation plate position for high throughput pipetting operations, and the two tube frame unit functional well positions are mirror images of each other.

The first pipe frame unit comprises 96 functional holes, 48 large functional holes can be inserted into the liquid collecting pipe, and the other 48 small functional holes and the large functional holes are arranged alternately. Each big functional hole is adjacent to 4 small functional holes in the upper, lower, left and right directions. Every small functional hole is adjacent and is 4 big functional holes about from top to bottom. The 48 small functional wells serve as spaces to accommodate the other 48 blank pipette tips of the 96 channel pipetting station that are not inserted into the 48 manifolds of the 1 st rack unit. The second pipe frame unit is mirror symmetrical with the first pipe frame unit, and the liquid samples 106 in the 96 liquid collecting pipes are just moved into the 96-hole microporous plate.

The working principle of the application is as follows: the 96-well microplate was constructed with 12 rows of wells numbered 1-12 in the transverse direction and 8 rows of wells numbered A-H in the longitudinal direction. The first pipe frame unit and the second pipe frame unit are mirror images of each other, each pipe frame unit comprises 96 functional holes, and according to the mode of 12-8, 12 transverse rows of holes are numbered by 1-12, 8 longitudinal rows of holes are numbered by A-H, and only the large functional holes and the small functional holes are arranged at intervals.

The first pipe frame unit and the second pipe frame unit have the same size, and the positions of the large and small functional holes are in mirror symmetry (for example, the positions of A, C, E, G rows of large functional holes of the first pipe frame unit correspond to 2, 4, 6, 8, 10 and 12 of a 96-hole plate, B, D, F, H rows of large functional holes of 1, 3, 5, 7, 9 and 11 of the second pipe frame unit are in mirror symmetry, A, C, E, G rows of large functional holes correspond to 1, 3, 5, 7, 9 and 11 of the 96-hole plate, and B, D, F, H rows of large functional holes of 2, 4, 6, 8, 10 and 12 of the 96-hole plate). The two scaffolds thus complemented fill the 96-well plate sample sites.

A pipe rack, comprising:

the first pipe frame unit comprises a plurality of first functional holes and a plurality of second functional holes, and the number of the first functional holes is the same as that of the second functional holes;

the first functional holes and the second functional holes are alternately arranged along a first direction, and any two first functional holes or any two second functional holes are not adjacent along the first direction;

the first functional holes and the second functional holes are alternately arranged along a second direction, and any two first functional holes or any two second functional holes are not adjacent along the second direction;

the first functional hole is used for placing a liquid collecting pipe, and the second functional hole is used for inserting a liquid-transfering gun head;

the first direction is perpendicular to the second direction.

Compared with the prior art, above-mentioned pipe support is through setting up a plurality ofly first function hole and a plurality of second function hole utilizes first function hole with the cooperation in second function hole can make place in liquid in the collecting main in the first function hole can the quick removal of high flux get to the work efficiency who moves the liquid work station has been increased.

Drawings

FIG. 1 is a process for rapid sampling by the present application of a pipetting station or gun;

FIG. 2 is a top view of a tube rack provided in accordance with an embodiment of the present application;

fig. 3 is a front view of a tube rack provided in an embodiment of the present application.

10 pipe frame

100 first pipe rack unit

101 first direction

102 second direction

103 liquid collecting pipe

104 pipette tip

105 micro-porous plate

106 liquid sample

111 first function hole

112 second function hole

200 second pipe rack unit

Detailed Description

In order to make the aforementioned objects, features and advantages of the present application more comprehensible, embodiments accompanying the present application are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. This application is capable of embodiments in many different forms than those described herein and those skilled in the art will be able to make similar modifications without departing from the spirit of the application and it is therefore not intended to be limited to the embodiments disclosed below.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1, a 96 channel pipetting station pipette tip is shown in elevation. The pipetting workstation samples from the first pipe support unit 100, and at the moment, pipette tips No. I, No. III, No. V, No. C and No. ⑪ in the pipette tips 104 are inserted into the liquid collecting pipe 103 of the first pipe support unit 100 to take liquid; the pipette tips No. two, four, eight and ⑫ in the pipette tip 104 are inserted into the small functional hole (i.e. the second functional hole 112) in the first pipe rack unit 100, and there is no sampling and no contamination. The pipetting station then moves the tips to the target microplate 105 and pumps the samples into the corresponding wells of the microplate 105.

The pipetting station then moves the pipette tips 104 into the second rack unit 200. At this time, the pipette tips No. two, four, eight, ⑫ in the pipette tip 104 are inserted into the liquid collecting tube 103 in the second tube rack unit 200 to take the liquid. The samples are then driven into corresponding wells of the same target microplate 105. The samples in 96 liquid collecting tubes 103 (test tubes, centrifuge tubes, blood collecting tubes and the like) can be moved into one microplate 105 plate by pipetting twice, so that a large amount of time is saved for subsequent experiments. The sampling plate can be operated by using a liquid-transfering discharging gun under the condition that a 96-channel liquid-transfering work station is not arranged, so that the sample transferring speed is greatly accelerated.

This application divide into two pipe support units, first pipe support unit 100 and second pipe support unit 200. Each tube frame unit size conforms to the microplate ANSI/SBS1-2004 standard, can be placed on a pipetting workstation plate position for high throughput pipetting operations, and the two tube frame unit functional well positions are mirror images of each other.

In one embodiment, the first tube rack unit 100 includes 96 functional holes, 48 large functional holes (i.e., the first functional holes 111) can be inserted into the header pipe 103, and another 48 small functional holes (i.e., the second functional holes 112) are arranged alternately with the large functional holes. Each big functional hole is adjacent to 4 small functional holes in the upper, lower, left and right directions. Every small functional hole is adjacent and is 4 big functional holes about from top to bottom. The 48 small functional wells serve to accommodate the other 48 blank pipette tips 104 of the 96 channel pipetting stations not inserted into the 48 collection tubes 103 of the first rack unit 100. The second tube rack unit 200 is mirror symmetrical to the first tube rack unit 100, and exactly moves the liquid samples 106 in the 96 liquid collecting tubes 103 into the 96-well microporous plate 105.

The working principle of the application is as follows: the 96-well microplate 105 was constructed with 12 rows of wells numbered 1-12 in the transverse direction and 8 rows of wells numbered a-H in the longitudinal direction. A first pipe rack unit 100 and a second pipe rack unit 200 which are mirror images of each other. Each tube frame unit contains 96 functional holes, and according to the mode of 12 × 8, 12 transverse rows of holes are numbered from 1 to 12, 8 longitudinal rows of holes are numbered from A to H, and only the large functional holes and the small functional holes are arranged at intervals.

The first pipe frame unit 100 and the second pipe frame unit 200 have the same size, and the positions of the large and small functional holes are mirror-symmetrical (for example, the positions of A, C, E, G rows of large functional holes are 2, 4, 6, 8, 10, 12, B, D, F, H rows are 1, 3, 5, 7, 9, 11, the positions of A, C, E, G rows of large functional holes are 1, 3, 5, 7, 9, 11, and the positions of B, D, F, H rows are 2, 4, 6, 8, 10, 12, corresponding to 96-well plates). The two thus complementary racks fill the 96-well microplate 105 sample sites.

Referring to fig. 2 and 3, an embodiment of the present application provides a pipe rack 10 including a first pipe rack unit 100 and a second pipe rack unit 200. The first pipe rack unit 100 includes a plurality of first function holes 111 (i.e., large function holes) and a plurality of second function holes 112 (i.e., small function holes), and the number of the first function holes 111 is the same as the number of the second function holes 112. The first functional holes 111 and the second functional holes 112 are alternately arranged along the first direction 101, and any two of the first functional holes 111 or any two of the second functional holes 112 are not adjacent to each other along the first direction 101.

The first functional holes 111 and the second functional holes 112 are alternately arranged along the second direction 102, and any two of the first functional holes 111 or any two of the second functional holes 112 are not adjacent to each other along the second direction 102. The first functional hole 111 is used for placing the liquid collecting pipe 103. The second functional hole 112 is for insertion of a pipette tip 104. The first direction 101 is perpendicular to the second direction 102. The second pipe support unit 200 is mirror-symmetrical to the first pipe support unit 100 along the first direction 101.

In one embodiment, the liquid collection tube 103 may be a test tube or centrifuge tube or a blood collection tube, among others.

In one embodiment, each large functional hole is 4 small functional holes adjacent to each other, i.e. any two of the first functional holes 111 along the first direction 101 or the second direction 102 are not adjacent to each other. In one embodiment, each small functional hole is 4 large functional holes, i.e. any two of the second functional holes 112 along the first direction 101 or the second direction 102 are not adjacent.

In one embodiment, that any two of the first functional holes 111 or any two of the second functional holes 112 are not adjacent along the first direction 101 means that: the functional holes along the first direction 101 are in sequence: the first functional hole 111, the second functional hole 112, and the like.

In one embodiment, that any two of the first functional holes 111 or any two of the second functional holes 112 are not adjacent along the second direction 102 means: the functional holes along the second direction 102 are in turn: the first functional hole 111, the second functional hole 112, and the like.

In one embodiment, a plurality of the second functional holes 112 (i.e., small functional holes) function to: the remaining empty pipette tips 104 that accommodate multiple pipette stations not inserted into the plurality of liquid collection tubes 103 in the first tube rack unit 100. In one embodiment, the second functional hole 112 may be circular in shape.

In one embodiment, the first functional holes 111 and the second functional holes 112 are alternately arranged along the first direction 101, which means that: the first functional holes 111, the second functional holes 112, the first functional holes 111, and the second functional holes 112 are arranged along the first direction 101. In one embodiment, the first functional holes 111 and the second functional holes 112 are alternately arranged along the second direction 102, which means that: the first functional holes 111, the second functional holes 112, the first functional holes 111, and the second functional holes 112 are arranged along the second direction 102.

In one embodiment, the first direction 101 may be a transverse direction or a longitudinal direction, and a specific direction is selected and can be selected according to actual requirements. In one embodiment, the second direction 102 may be a transverse direction or a longitudinal direction. In one embodiment, if the first direction 101 is a transverse direction, the second direction 102 is a longitudinal direction. In one embodiment, if the first direction 101 is a longitudinal direction, the second direction 102 is a transverse direction.

In one embodiment, the first pipe rack unit 100 and the second pipe rack unit 200 are each provided with an identification code having an identification function. The first pipe rack unit 100 and the second pipe rack unit 200 can be quickly distinguished by the identification code. It is to be understood that the type of the identification code is not limited as long as the first pipe rack unit 100 and the second pipe rack unit 200 can be quickly distinguished by the identification code. In one embodiment, the identification code may be a bar code. In one embodiment, the identification code may be a two-dimensional code.

Referring to fig. 2 and 3, another embodiment of the present application provides a pipe rack 10 including a first pipe rack unit 100 and a second pipe rack unit 200. The first pipe rack unit 100 includes a plurality of first function holes 111 (i.e., large function holes) and a plurality of second function holes 112 (i.e., small function holes). The first functional holes 111 and the second functional holes 112 are alternately arranged along the first direction 101, and any two of the first functional holes 111 or any two of the second functional holes 112 are not adjacent to each other along the first direction 101. The first functional holes 111 and the second functional holes 112 are alternately arranged along the second direction 102, and any two of the first functional holes 111 or any two of the second functional holes 112 are not adjacent to each other along the second direction 102. The second pipe support unit 200 is mirror-symmetrical to the first pipe support unit 100 along the first direction 101.

In one embodiment, the first functional hole 111 has a larger aperture than the second functional hole 112

In one embodiment, a plurality of the second functional holes 112 (i.e., small functional holes) function to: the remaining empty pipette tips 104 that accommodate multiple pipette stations not inserted into the plurality of liquid collection tubes 103 in the first tube rack unit 100.

In one embodiment, the multi-well microplate may be configured with 12 rows of wells numbered 1-12 in the transverse direction and 8 rows of wells numbered A-H in the longitudinal direction. The first tube frame unit 100 can be designed with 96 functional holes, and 12 rows of holes are numbered 1-12 in the transverse direction, 8 rows of holes are numbered a-H in the longitudinal direction, and only the functional holes with different sizes are arranged at intervals according to the 12-by-8 mode.

In one embodiment, each of the first pipe rack units 100 is provided with an identification code having an identification function. Each of the first pipe rack units 100 can be quickly identified by the identification code. It is to be understood that the type of the identification code is not limited as long as each of the first pipe rack units 100 can be quickly identified by the identification code. In one embodiment, the identification code may be a bar code. In one embodiment, the identification code may be a two-dimensional code.

To sum up, this application is through setting up a plurality ofly first function hole 111 and a plurality of second function hole 112, utilize first function hole 111 with second function hole 112's cooperation can make place in the liquid in the collector tube 103 in first function hole 111 can the quick removal of high flux to the work efficiency of moving liquid work station has been increased.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims

1. A pipe rack, comprising:

a first pipe rack unit (100) including a plurality of first functional holes (111) and a plurality of second functional holes (112), and the number of the first functional holes (111) is the same as the number of the second functional holes (112);

the first functional holes (111) and the second functional holes (112) are alternately arranged along a first direction (101), and any two first functional holes (111) or any two second functional holes (112) are not adjacent along the first direction (101);

the first functional holes (111) and the second functional holes (112) are alternately arranged along a second direction (102), and any two first functional holes (111) or any two second functional holes (112) are not adjacent along the second direction (102);

the first functional hole (111) is used for placing a liquid collecting pipe (103), and the second functional hole (112) is used for inserting a pipette tip (104);

the first direction (101) is arranged perpendicular to the second direction (102).

2. The pipe rack of claim 1, further comprising:

a second pipe rack unit (200) mirror-symmetrical to the first pipe rack unit (100) along the first direction (101).

3. Pipe support according to claim 2, characterized in that the first functional pores (111) have a larger pore size than the second functional pores (112); the first pipe frame unit (100) and the second pipe frame unit (200) are provided with identification codes with identification functions.

4. The pipe rack of claim 3, wherein the identification code is a bar code or a two-dimensional code.

5. A pipe rack, comprising:

a first pipe rack unit (100), the first pipe rack unit (100) including a plurality of first functional holes (111) and a plurality of second functional holes (112);

the first functional holes (111) and the second functional holes (112) are alternately arranged along a second direction (102), and any two of the first functional holes (111) or any two of the second functional holes (112) are not adjacent to each other along the second direction (102).

6. The pipe rack of claim 5, further comprising:

7. Pipe support according to claim 5, characterized in that the first functional pores (111) have a larger pore size than the second functional pores (112).

8. Pipe support according to claim 7, characterized in that said first functional holes (111) have the same number as said second functional holes (112) and are arranged in 8 rows of said first direction (101) and 12 columns of said second direction (102).

9. Pipe rack according to claim 5, characterized in that the first pipe rack unit (100) is provided with an identification code having an identification function.

10. The pipe rack of claim 9, wherein the identification code is a bar code or a two-dimensional code.