CN109602905A - Amphipathic molecule and Ag2Application of the micella that S quantum dot is formed as photosensitizer - Google Patents
Amphipathic molecule and Ag2Application of the micella that S quantum dot is formed as photosensitizer Download PDFInfo
- Publication number
- CN109602905A CN109602905A CN201811510208.2A CN201811510208A CN109602905A CN 109602905 A CN109602905 A CN 109602905A CN 201811510208 A CN201811510208 A CN 201811510208A CN 109602905 A CN109602905 A CN 109602905A
- Authority
- CN
- China
- Prior art keywords
- micella
- amphipathic
- quantum dot
- application
- molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
- A61K41/0057—Photodynamic therapy with a photosensitizer, i.e. agent able to produce reactive oxygen species upon exposure to light or radiation, e.g. UV or visible light; photocleavage of nucleic acids with an agent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
- A61K9/1075—Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Abstract
The invention discloses amphipathic molecules and Ag2Application of the micella that S quantum dot is formed as photosensitizer, belongs to technical field of nano material.Amphipathic molecule and Ag2For the micella that S quantum dot is formed as the application for preparing antitumor photosensitizer, the amphipathic molecule is amphiphilic polymer or amphipathic protein, including amphipathic phospholipid molecule.For the micella under near infrared light excitation, state is transformed into excitation state by ground state, reacts with oxygen molecule around, and oxygen molecule is made to generate toxicity photochemical product.The power density of the near infrared light is 1w/cm2‑2.0w/cm2.Amphipathic molecule and Ag in the present invention2The micella that S quantum dot is formed has the ability of the light activated Photodynamic therapy tumour cell of near-infrared, has good blood compatibility, solves the technical issues of phototoxicity, treatment depth as shallow of existing photosensitizer.
Description
Technical field
The invention belongs to technical field of nano material, and in particular to arrive amphipathic molecule and Ag2The glue that S quantum dot is formed
The application of Shu Zuowei photosensitizer.
Background technique
One of the main reason for cancer is threat human life and health, therefore the Clinics of cancer have great society
It can demand.Photodynamic therapy is one developed rapidly in recent years in therapeutic field of tumor as a kind of Minimal invasive procedures
Kind new technology, it has also become one of most active research field in treatment and prevention of tumour science.The basic principle is that utilizing sensitiser absorption
External source is visible or near infrared light energy, itself is changed into after excitation state to react with surrounding molecular oxygen by ground state and generates high activity excitation
State toxicity photochemical product such as active oxygen radical etc., then direct killing tumor cell induction Apoptosis or necrosis, or destroy
Blood vessel in mesenchyma stroma of tumors leads to tumor ischemia anoxic etc., while can also start the antitumor effect of specific immune mechanisms enhancing
Fruit.
Currently, with the fast development of photodynamic therapy, in the light source and photosensitizer wherein to play a decisive role also not
It is disconnected to update.For light source, the appearance for minimizing semiconductor laser overcomes early stage ruby laser volume Pang
Greatly, the poor disadvantage of stability, light source are substantially achieved solution to the limitation of photodynamic therapy.On the whole, the longer light pair of wavelength
The penetration capacity of tissue is stronger, the light near 600,700 and 800nm of wavelength, 0.5 respectively may be about to the penetration depth of tissue,
0.8 and 1cm, thus with the increase of wavelength in human body optical window, photodynamic therapeutic domain is also bigger.For photosensitive
For agent, the shortcomings that overcoming first generation photosensitizer to the photosensitizing moiety of second generation porphyrin, the photosensitive phase is shorter, absorbs wave
Length is longer, and skin phototoxicity is lower, therefore has obtained more application.However, causing to control since its absorbing wavelength still falls short of
It is still not deep enough to treat depth, and the phototoxicity still having, it is unfavorable that these all produce its further application clinically
It influences.Therefore finding, there is the photosensitizer of longer absorbing wavelength to keep penetration depth more preferable, for expanding the application of photodynamic therapy
It is of great significance.
The development of nanotechnology make nano material it is increased appear in field of biomedicine, and play increasingly
Important role, with the fast development of photodynamic therapy, the application in the field is also more noticeable.Quantum dot with
It has been obtained in field of biomedicine compared with ten-strike by means of its excellent optical characteristics.It, can be real such as newest less toxic silver sulfide quantum dot
Existing wide wavelength range absorbs, and fluorescent emission can reach 2nd area of near-infrared (1000~1300nm), therefore can be in small animal living body
It plays a significant role in fluorescence imaging.Research also shows that silver sulfide quantum dot also has photo-thermal effect at present, thus be it is a kind of not
It can the multi-functional nanometer materials that obtained more.And currently, being had not been reported to the research of the light power behavior of silver sulfide quantum dot.
Summary of the invention
The present invention solves photosensitizing agents depth as shallow in the prior art, and there are phototoxic technical problems, originally
The good amphipathic molecule of biocompatibility and Ag in invention2The micella that S quantum dot is formed, certain density this micella exist
Under near infrared light excitation induction, this micella is changed into excitation state by ground state, then anti-with the oxygen molecule in ambient enviroment
Answer, generate virose singlet oxygen, this singlet oxygen can directly inducing apoptosis of tumour cell or necrosis, or destroy
Blood vessel in mesenchyma stroma of tumors leads to tumor ischemia anoxic etc., while can also start specific immune mechanisms enhancing antitumous effect.
Purpose according to the invention, provides amphipathic molecule and Ag2S quantum dot formed micella prepare it is antitumor
Application in photosensitizer.
Preferably, the amphipathic molecule is amphiphilic polymer or amphipathic protein.
Preferably, the amphiphilic polymer is amphipathic phosphatide.
Preferably, the amphipathic phosphatide is the copolymer of Distearoyl Phosphatidylethanolamine and polyethylene glycol, described poly-
Ethylene glycol is end modified amino.
Preferably, the micella is under near infrared light excitation, and state is transformed into excitation state by ground state, with oxygen molecule around
It reacts, oxygen molecule is made to generate toxicity photochemical product.
Preferably, the toxicity photochemical product is singlet oxygen.
Preferably, the wavelength of the near infrared light is 700nm-900nm.
Preferably, the power density of the near infrared light is 1w/cm2-2.0w/cm2。
Preferably, the diameter of the micella is 10nm-20nm.
Preferably, Ag in the micella2The concentration of S quantum dot is 20 μ g/mL-60 μ g/mL.
In general, through the invention it is contemplated above technical scheme is compared with the prior art, mainly have below
Technological merit:
(1) method that clinically treatment of tumour mostly uses chemotherapy radiotherapy one kind, this causes great negative shadow to patient
It rings, while radical curing of disease, on patient body, is psychologically also subject to very big wound.And in recent years, minimally-invasive treatment development is fast
Speed, it is smaller to patient's negative effect while curing disease, just compensate for this deficiency.Optical dynamic therapy is as a kind of
Minimally-invasive treatment mode possesses broad application prospect, it itself is changed by ground state sharp using sensitiser absorption external source luminous energy
It is reacted after hair state with surrounding molecular oxygen and generates high activity excitation state toxicity photochemical product such as active oxygen radical etc., then directly
Killing tumor cell induces cell apoptosis or necrosis, or the blood vessel destroyed in mesenchyma stroma of tumors leads to tumor ischemia anoxic etc., simultaneously
Specific immune mechanisms enhancing antitumous effect can also be started.Two generation photosensitizers having been commercialized at present are mainly derivative with porphyrin
Based on object, but the maximum defect of porphyrin analog derivative is that required light source is visible light, and it is deep unavoidably to will cause treatment
Shallow, phototoxicity problems are spent, therapeutic effect is not thorough and damages normal skin and tissue.And use porphyrin patient necessary
It is chronically under the conditions of being protected from light, for long-term treatment, dark can equally be impacted the psychology of patient for this.The present invention
The amphipathic molecule and Ag2The micella that S quantum dot is formed is as novel photosensitive agent probe, and size is uniform, and stability is good,
The high probe that can be used for optical dynamic therapy of biocompatibility.
(2) amphipathic molecule and Ag in the present invention2The micella that S quantum dot is formed is induced under near infrared light excitation and is generated
Singlet oxygen, compared with the prior art present in excited by visible light photosensitizer light permeability shallowly so as to cause treat it is not thorough
Bottom problem, near-infrared excitation can be solved effectively, and excitation wavelength longer (700nm-900nm) is, it can be achieved that deeper control
It treats.Profound treatment is this means that in the living body, after photosensitizer reaches certain space depth by ringing, exciting light
Source still is able to excitation photosensitizer to generate photosensitizing efficiency, and to the light of different wavelength, living body penetration depth is
Have differences, basic reason is, the substances such as intravital hemoglobin to the light absorption in visible wavelength range compared with
It is more, it will cause the decaying of excessive light energy in this way, fine penetration depth is not achieved in light, and near infrared light can make up this
Problem.
Detailed description of the invention
Fig. 1 is the signal for turning water after cure silver and detecting on electron spin resonance spectrometer of embodiment 1.
Fig. 2 be embodiment 2 turn water after cure silver and after DPBF is incubated for altogether, after different laser irradiation times, DPBF
Abosrption spectrogram.
Fig. 3 is that the water after cure silver probe that turns of embodiment 3 is incubated for altogether with HeLa cell, and passes through 808nm wavelength laser
After processing, measured DCF fluorescence emission spectrogram of compound.
Fig. 4 is that the water after cure silver probe that turns of embodiment 3 is incubated for altogether with HeLa cell, at 808nm wavelength laser
After reason, measured DCF fluorescent microscopic imaging figure: wherein Fig. 4 (a) is fluorescence grayscale image under the conditions of PBS, and Fig. 4 (b) is PBS item
White light figure under part, Fig. 4 (c) are to turn fluorescence grayscale image under the conditions of water after cure silver quantum dot, and Fig. 4 (d) is to turn water after cure silver content
White light figure under the conditions of son point.
Fig. 5 is that the water after cure silver that turns of embodiment 4 is incubated for altogether with three kinds of tumour cells, and passes through 808nm wavelength laser
After processing, measured cell survival rate.
Fig. 6 be embodiment 5 turn water after cure silver intratumor injection to Mice Body it is interior after, the tumour body after laser treatment
Product is with different time change curve.
Fig. 7 is that embodiment 5 turns water after cure silver intratumor injection to after in Mice Body, and by laser treatment, treatment is observed
After the final white light figure of tumour.After wherein Fig. 7 (a) is the processing of intratumor injection PBS condition lower observation 20 days, by three
Tumor locus white light figure after mouse is put to death, Fig. 7 (b) is that PBS adds under lasing condition processing after observation 20 days, small by three
Tumor locus white light figure after mouse is put to death, Fig. 7 (c) are to turn water after cure silver quantum dot lower observation 20 days by lasing condition processing
After, tumor locus white light figure after three mouse are put to death.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments,
The present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain this hair
It is bright, it is not intended to limit the present invention.In addition, technology involved in the various embodiments of the present invention described below is special
Sign can be combined with each other as long as they do not conflict with each other.
Amphipathic molecule and Ag2The micella that S quantum dot is formed is as antitumor photosensitizer, Ag2S quantum dot is oil-soluble
, the hydrophobic end of amphipathic molecule envelopes oil-soluble Ag towards inside2S quantum dot, the water-wet side court of amphipathic molecule
Outside, the micella obtained is hydrophilic.Amphipathic molecule is not limited to distearoylphosphatidylethanolamine-polyethylene glycol-ammonia
Base, other such as amphipathic protein, amphipathy macromolecule are all within this range.The particle size of oil solubility nanometer silver sulfide
For 2-5nm；The near infrared region launch wavelength of oil solubility nanometer silver sulfide quantum dot is 1000nm-1300nm.
Silver sulfide nanometer particles quantum dot is obtained according to following preparation method:
(1) by silver diethyl dithio carbamate, octadecylene, lauryl mercaptan according to molar ratio 3:1:0.3 and indifferent gas
It is uniformly mixed within body protection lower stirring 30 minutes.
(2) mixed system in step 1 100 DEG C are warming up to be kept for 10 minutes.
(3) mixed system in step 2 165 DEG C are warming up to be kept for 10 minutes.
(4) by mixed system in step 3 with n-hexane: acetone volume ratio 1:3 centrifugal purification.
(5) disperse precipitating chloroform obtained in step 4 to obtain silver sulfide quantum dot.
Phosphatide and oil-soluble Ag2The micella that S quantum dot is formed is obtained according to following preparation method: by 6mg DSPE-
mPEG2000-NH2(distearoylphosphatidylethanolamine-polyethylene glycol-amino) is added to 10 mL and contains 15mg oiliness Ag2S amount
In son point, 8-15min is stirred at room temperature in ultrasonic 10min, and solvent is slowly drained with vacuum pump, is obtained solid film and is invested tube wall,
Ultrapure water dissolves to obtain aqueous phospholipid and oil-soluble Ag2The micella that S quantum dot is formed, the partial size of micella are 10-20nm.Glue
Beam is using oil-soluble silver sulfide quantum dot as core, and using hydrophobic forces, amphipathic molecule is coated on quantum dot surface, shape
At flexible vesica.The partial size of the oil-soluble silver sulfide quantum dot is in 2-5nm, and launch wavelength is in 1000-1300nm, tool
There is good stability.Probe hydration partial size after turning water is in 10-20nm, and distribution is uniform, and stability is good.Therefore, a this step
The nano-probe of method synthesis, it is easy to operate, it is easy large scale preparation.And this micella is induced under near infrared light excitation and is produced
Raw singlet oxygen.
Amphipathic molecule and Ag provided by the invention2The micella that S quantum dot is formed is after near infrared light excites, state
Excitation state is transformed by ground state, is reacted with oxygen molecule around, high activity excitation state toxicity photochemical product is generated, it is such as single
Line state oxygen induces cell apoptosis and downright bad.
Embodiment 1: test generates singlet oxygen after turning the excitation of water after cure silver light in probe level
Association reaction can occur with agent for capturing TEMP using singlet oxygen, generate TEMPO, and TEMPO possesses unique three
Weight resonance signal peak.Bruker electron spin resonance spectrometer is as detection system, MDL-III-808-2.5W laser conduct
Excitation light source, spectroscopic data are obtained by control programmed acquisition.
Near-infrared silver sulfide quantum dot after turning water is as singlet oxygen test method produced by novel photosensitive agent probe:
It will turn water after cure silver-colored (15 μ g/mL) and singlet oxygen agent for capturing TEMP (2,2,6,6- tetramethyl piperidine) mixes,
In MDL-III-808-2.5W laser with 1.0w/cm2Lower photoinduction excites 10min, then in Bruker electron spin resonance
Signal is detected on spectrometer.
As a result as shown in Figure 1, it is known that singlet oxygen and agent for capturing are in magnetic field strength 3480-3540G model from Fig. 1
In enclosing, there are typical TEMPO triplet, illustrate that singlet oxygen generates.
Embodiment 2: singlet oxygen production is detected in probe level and changes over time situation
DPBF is one of highest singlet oxygen capturing agent of known activity, and in singlet oxygen meeting attack DPBF structure
Furan nucleus is allowed to open, and absorption of the DPBF at 410nm is caused to change.Therefore, by by DPBF and turning water after cure silver
It is incubated for altogether, respectively after the laser irradiation of different time, acquires absorption spectrum in UV-2550 ultraviolet-uisible spectrophotometer
Figure.
Near-infrared silver sulfide quantum dot after turning water generates at any time as singlet oxygen caused by novel photosensitive agent probe
Between change the case where test method it is as follows:
The water after cure silver-colored (15 μ g/mL) that turns of 2.5mL is taken in quartz colorimetric utensil, the DPBF (1,3- of 100 μ L is added
Diphenyl isobenzofuran), by MDL-III-808-2.5W laser 1.0w/cm2Induced with laser different time (0,2,12,
20,30,42 and 56min) after, UV-2550 ultraviolet-uisible spectrophotometer spectra re-recorded.
As a result as shown in Fig. 2, from Fig. 2 it is known that with the induced with laser time extension, the furan nucleus of DPBF is not
It is disconnected to be destroyed, cause its absorption at 410nm constantly to decline, further proves the generation of singlet oxygen.
Embodiment 3: singlet oxygen production is detected in cell level
Known DCFH-DA is as reactive oxygen fluorescence detection kit.After DCFH-DA is diffused into the cell, it can slough
Acetyl group becomes the DCFH without fluorescence, and DCFH can be promptly oxidized to by active oxygen with high-intensitive fluorescence
DCF, and fluorescence intensity is directly proportional to ROS content in cell liquid.Therefore, by HeLa cell and after turning water, probe is total
After incubation, and after MDL-III-808-2.5W laser excites photoinduction, recorded by QE6500 Fluorescence Spectrometer different
Fluorescence spectra emission situation, also, the cell fluorescence imaging contexts handled by fluorescence microscope different modes, to examine
It surveys evoked by probe singlet oxygen and generates ability.
Near-infrared silver sulfide quantum dot after turning water is as singlet oxygen caused by novel photosensitive agent probe cell level
Test method is as follows:
By the HeLa cell of logarithmic growth phase, be separately added into 500 μ L turns water after cure silver (Ag2S concentration is 40 μ g/
ML) serum free medium is absorbed culture medium PBS after 6h and is cleaned 3 times, DCFH-DA (2', the 7'- dichloro that 500 μ L contain 10 μM is added
Fluorescein(e) diacetate) serum free medium, with 808nm laser 1w/cm210min is induced, PBS after incubation 0.5h is continued
It cleans 3 times and removes free DCFH-DA, the fixed 30min rear portion of 2.5% glutaraldehyde is in fluorescence microscopy microscopic observation, separately
A part collects cell with cell scraper, is then in excitation wavelength by cell collected by fluorescent spectrophotometer assay
The emission spectrum of 488nm.
Fig. 3 is that the water after cure silver probe that turns of embodiment 3 is incubated for altogether with HeLa cell, and passes through 808nm wavelength laser
After processing, measured DCF fluorescence emission spectrogram of compound.By turning water after cure silver compared with the control group it can be found that after turning water
The fluorescence signal intensity that silver sulfide generates is much larger than PBS control group, this explanation turns water after cure silver and generates with induced activity oxygen
Ability, and there is also fluorescence intensities for PBS group, this is because inherently there is living radical into the cell.After turning water
Fig. 4 is that the water after cure silver probe that turns of embodiment 3 is incubated for altogether with HeLa cell, at 808nm wavelength laser
After reason, measured DCF fluorescent microscopic imaging figure: wherein Fig. 4 (a) is fluorescence grayscale image under the conditions of PBS, and Fig. 4 (b) is PBS item
White light figure under part, Fig. 4 (c) are to turn fluorescence grayscale image under the conditions of water after cure silver quantum dot, and Fig. 4 (d) is to turn water after cure silver content
White light figure under the conditions of son point.It is produced by turning water after cure silver compared with the control group it can be found that turning water after cure silver group
More green fluorescences (the brighter place of gray scale), illustrate the generation for turning water after cure silver group singlet oxygen.
Embodiment 4: in cell level, detection turns the light power Cell killing efficacy of water after cure silver
It standardizes MTT and detects cell survival rate.By three kinds of tumour cells (HeLa, 4T1, MCF-7) and turn water after cure
It is silver-colored to be incubated for altogether, after MDL-III-808-2.5W laser excites photoinduction, exist in Elx-808 microplate reader measurement cell
It is absorbed at 490nm, to calculate evoked by probe Apoptosis result.
Near-infrared silver sulfide quantum dot after turning water is as singlet oxygen cell killing caused by novel photosensitive agent probe
The test method of effect is as follows:
By HeLa, MCF-7 and 4T1 cell of logarithmic growth phase, it is separately added into and turns water after cure silver (Ag containing 200 μ L2S
Culture medium PBS is removed for the serum free medium of 40 μ g/mL), after 4h to clean 3 times, fresh culture is added, with 808nm laser
(1w/cm2) radiation-induced 10min, continue to be incubated for thiazolyl blue (5mg/mL) the incubation 4h that 20 μ L are added afterwards for 24 hours, in microplate reader
It measures and is absorbed at 490nm, calculate cell survival rate.
Fig. 5 is that the water after cure silver that turns of embodiment 4 is incubated for altogether with three kinds of tumour cells, and passes through 808nm wavelength laser
After processing, measured cell survival rate.From three kinds of cell survival rates, blank group after induced with laser, cell
Survival rate is almost unchanged, and turns to produce apparent Apoptosis, lethality after water after cure galactic longitude crosses induced with laser
11.78%, 14.4%, 14.8% is respectively reached, it is poor that there are conspicuousnesses compared with PBS group and PBS add laser group survival rate difference
Different, this explanation, under the conditions of the concentration and probe concentration used in us and laser power density, the silver sulfide after turning water has good
Killing tumor cell effect, three kinds of cell lethalities are not much different, this illustrates the Cell killing efficacy for turning water after cure silver
With universality.After turning water.
Embodiment 5: in animal level, probe is observed on 4T1 tumor model and inhibits tumour growth situation
It is inoculated with 4T1 cell by BALB/c mouse, grows to 40-100mm to tumour3, after turning water by intratumor injection respectively
Probe (other auxiliary materials can also be added) and PBS solution excite photoinduction 10 minutes by MDL-III-808-2.5W laser
Afterwards, start record observation mouse tumor and weight size variation at regular intervals.
Fig. 6 be embodiment 5 turn water after cure silver intratumor injection to Mice Body it is interior after, the tumour body after laser treatment
Product is with different time change curve.From the figure, it can be seen that turn water after cure silver group after induced with laser, tumour
Growth is obviously suppressed, and final tumor average volume is close to 400mm3, and PBS processing group tumor average volume is
1465mm3, PBS add laser treatment group tumor average volume be 1668mm3, turn water after cure silver and laser group tumor suppression ratio added to reach
72.9%-76.2% is arrived, this, which is further demonstrated, turns water after cure silver in the therapeutic effect of living body.
Fig. 7 is that embodiment 5 turns water after cure silver intratumor injection to after in Mice Body, and by laser treatment, treatment is observed
After the final white light figure of tumour.After wherein Fig. 7 (a) is the processing of intratumor injection PBS condition lower observation 20 days, by three
Tumor locus white light figure after mouse is put to death, Fig. 7 (b) is that PBS adds under lasing condition processing after observation 20 days, small by three
Tumor locus white light figure after mouse is put to death, Fig. 7 (c) are to turn water after cure silver quantum dot lower observation 20 days by lasing condition processing
After, tumor locus white light figure after three mouse are put to death.It can find that two groups are finally swollen by Fig. 7 (a) and Fig. 7 (b)
Knurl product is not much different, and illustrates that the intervention of pure laser does not have substantive damage to mouse tumor.Pass through Fig. 7 (b) and Fig. 7 (c)
It can be seen that turning water after cure silver is injected into mouse tumor and after laser treatment, final only two mouse tumors are multiple
Hair, and gross tumor volume is significantly smaller than b, and other one does not recur, this explanation, the silver sulfide after turning water passes through laser treatment
Afterwards, inhibition and therapeutic effect are produced to tumour.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all wrap
Containing within protection scope of the present invention.
Claims (10)
1. amphipathic molecule and Ag2The micella that S quantum dot is formed is preparing the application in anti-tumor photosensitizer.
2. application as described in claim 1, which is characterized in that the amphipathic molecule is amphiphilic polymer or amphipathic egg
White matter.
3. application as claimed in claim 2, which is characterized in that the amphiphilic polymer is amphipathic phosphatide.
4. application as claimed in claim 3, which is characterized in that the amphipathic phosphatide be Distearoyl Phosphatidylethanolamine with
The copolymer of polyethylene glycol, the polyethylene glycol is end modified amino.
5. application as described in claim 1, which is characterized in that under near infrared light excitation, state is turned the micella by ground state
Become excitation state, react with oxygen molecule around, oxygen molecule is made to generate toxicity photochemical product.
6. application as claimed in claim 5, which is characterized in that the toxicity photochemical product is singlet oxygen.
7. application as claimed in claim 5, which is characterized in that the wavelength of the near infrared light is 700nm-900nm.
8. application as claimed in claim 5, which is characterized in that the power density of the near infrared light is 1w/cm2-2.0w/
cm2。
9. application as described in claim 1, which is characterized in that the diameter of the micella is 10nm-20nm.
10. application as described in claim 1, which is characterized in that Ag in the micella2The concentration of S quantum dot is 20 μ g/mL-60
μg/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811510208.2A CN109602905B (en) | 2018-12-11 | 2018-12-11 | Amphiphilic molecules with Ag2Application of micelle formed by S quantum dots as photosensitizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811510208.2A CN109602905B (en) | 2018-12-11 | 2018-12-11 | Amphiphilic molecules with Ag2Application of micelle formed by S quantum dots as photosensitizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109602905A true CN109602905A (en) | 2019-04-12 |
| CN109602905B CN109602905B (en) | 2022-03-29 |
Family
ID=66008765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811510208.2A Active CN109602905B (en) | 2018-12-11 | 2018-12-11 | Amphiphilic molecules with Ag2Application of micelle formed by S quantum dots as photosensitizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109602905B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105012970A (en) * | 2015-07-21 | 2015-11-04 | 福州市传染病医院 | Multifunctional nanomicelle for early diagnosis and phototherapy of tumors and application of multifunctional nanomicelle |
-
2018
- 2018-12-11 CN CN201811510208.2A patent/CN109602905B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105012970A (en) * | 2015-07-21 | 2015-11-04 | 福州市传染病医院 | Multifunctional nanomicelle for early diagnosis and phototherapy of tumors and application of multifunctional nanomicelle |
Non-Patent Citations (1)
| Title |
|---|
| 刘静: ""纳米金属硫(氧)化物的可控合成及其细胞生物学效应"", 《河南师范大学硕士学位论文》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109602905B (en) | 2022-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mohammad-Hadi et al. | Photodynamic therapy in 3D cancer models and the utilisation of nanodelivery systems | |
| Chan et al. | Cell uptake, distribution and response to aluminium chloro sulphonated phthalocyanine, a potential anti-tumour photosensitizer | |
| US9211283B2 (en) | Nanoparticle carrier systems based on human serum albumin for photodynamic therapy | |
| JP5566945B2 (en) | Water-soluble anionic bacteriochlorophyll derivatives and their use | |
| JP2004505040A (en) | Composition | |
| JP2001501970A (en) | Photochemotherapy composition | |
| Chou et al. | Femto-second laser beam with a low power density achieved a two-photon photodynamic cancer therapy with quantum dots | |
| KR102363327B1 (en) | Composition for photodynamic therapy containing 5-aminolevulinic acid hydrochloride | |
| Garapati et al. | Photodynamic therapy: A special emphasis on nanocarrier-mediated delivery of photosensitizers in antimicrobial therapy | |
| Chen et al. | NIR photosensitizer for two-photon fluorescent imaging and photodynamic therapy of tumor | |
| CN113559064A (en) | Novel self-oxygen-supply liposome nanoparticle and preparation method and application thereof | |
| Adnane et al. | The combinational application of photodynamic therapy and nanotechnology in skin cancer treatment: A review | |
| CN106310262A (en) | Gold nano-meso-porous silicon nanorod compound for phototherapy and imaging and preparation method thereof | |
| Brandis et al. | Bacteriochlorophyll sensitizers in photodynamic therapy | |
| Atif et al. | Role of sensitivity of zinc oxide nanorods (ZnO-NRs) based photosensitizers in hepatocellular site of biological tissue | |
| CN109602905A (en) | Amphipathic molecule and Ag2Application of the micella that S quantum dot is formed as photosensitizer | |
| CN108774249B (en) | Oxazine compound and application thereof | |
| CN114620756B (en) | Preparation method, product and application of gold-cerium oxide nano material | |
| CN114601925A (en) | Photosensitive nano material jointly modified by hyaluronic acid and RSL3, preparation method and application thereof | |
| CN114292639A (en) | Multifunctional nano material based on aggregation-induced emission and MXenes, preparation method and application | |
| CN113230419A (en) | Novel targeted nano-particles based on phycocyanobilin and preparation method thereof | |
| He et al. | Poly (N-isopropylacrylamide)-coated thermo-responsive nanoparticles for controlled delivery of sulfonated Zn-phthalocyanine in Chinese hamster ovary cells in vitro and zebra fish in vivo | |
| CN115465853B (en) | Orange light carbon dot based on citric acid and chiral 2-amino-1, 2-diphenyl ethanol and preparation method and application thereof | |
| CN115490672B (en) | Photosensitizer with photothermal and photodynamic effects as well as preparation method and application thereof | |
| CN113181357B (en) | Organic nano tumor photothermal agent and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |