CN109601378B - Low-temperature preservation method and cultivation method for seed source of sugarcane tissue culture seedling - Google Patents
Low-temperature preservation method and cultivation method for seed source of sugarcane tissue culture seedling Download PDFInfo
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- CN109601378B CN109601378B CN201811453475.0A CN201811453475A CN109601378B CN 109601378 B CN109601378 B CN 109601378B CN 201811453475 A CN201811453475 A CN 201811453475A CN 109601378 B CN109601378 B CN 109601378B
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- temperature
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- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 64
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 56
- 238000004321 preservation Methods 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000012364 cultivation method Methods 0.000 title claims abstract description 7
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000000661 sodium alginate Substances 0.000 claims abstract description 24
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 24
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 24
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 21
- 238000005520 cutting process Methods 0.000 claims abstract description 19
- 239000000126 substance Substances 0.000 claims abstract description 10
- 238000002791 soaking Methods 0.000 claims abstract description 8
- 239000001110 calcium chloride Substances 0.000 claims abstract description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims description 45
- 238000005286 illumination Methods 0.000 claims description 33
- 238000011084 recovery Methods 0.000 claims description 27
- 229930006000 Sucrose Natural products 0.000 claims description 19
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 19
- 229960004793 sucrose Drugs 0.000 claims description 19
- 229920001817 Agar Polymers 0.000 claims description 17
- 239000008272 agar Substances 0.000 claims description 17
- 239000002904 solvent Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- CGIDKJRJBMFXKV-UHFFFAOYSA-N 6-n'-benzylpurine-6,6-diamine Chemical compound N1=CN=C2N=CN=C2C1(N)NCC1=CC=CC=C1 CGIDKJRJBMFXKV-UHFFFAOYSA-N 0.000 claims description 16
- 230000035755 proliferation Effects 0.000 claims description 15
- 201000010099 disease Diseases 0.000 claims description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 10
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 10
- 230000000977 initiatory effect Effects 0.000 claims description 9
- 239000000499 gel Substances 0.000 claims description 8
- 239000012883 rooting culture medium Substances 0.000 claims description 8
- 230000000249 desinfective effect Effects 0.000 claims description 6
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 5
- 239000005985 Paclobutrazol Substances 0.000 claims description 5
- 241000700605 Viruses Species 0.000 claims description 5
- 244000052616 bacterial pathogen Species 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 230000001900 immune effect Effects 0.000 claims description 5
- 239000008223 sterile water Substances 0.000 claims description 5
- 239000002352 surface water Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 230000000644 propagated effect Effects 0.000 claims description 2
- 238000005138 cryopreservation Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 1
- 238000003860 storage Methods 0.000 abstract description 10
- 230000002035 prolonged effect Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000012879 subculture medium Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004790 biotic stress Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000010485 coping Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000006870 ms-medium Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000002633 protecting effect Effects 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/55—Sugar cane
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811453475.0A CN109601378B (en) | 2018-11-30 | 2018-11-30 | Low-temperature preservation method and cultivation method for seed source of sugarcane tissue culture seedling |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201811453475.0A CN109601378B (en) | 2018-11-30 | 2018-11-30 | Low-temperature preservation method and cultivation method for seed source of sugarcane tissue culture seedling |
Publications (2)
Publication Number | Publication Date |
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CN109601378A CN109601378A (en) | 2019-04-12 |
CN109601378B true CN109601378B (en) | 2021-08-24 |
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CN201811453475.0A Expired - Fee Related CN109601378B (en) | 2018-11-30 | 2018-11-30 | Low-temperature preservation method and cultivation method for seed source of sugarcane tissue culture seedling |
Country Status (1)
Country | Link |
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CN (1) | CN109601378B (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1252685A (en) * | 1997-02-21 | 2000-05-10 | 莱拉·扎卡里亚·阿布德拉曼 | Sugar cane production |
CN102217537A (en) * | 2011-04-20 | 2011-10-19 | 中国科学院华南植物园 | Low-temperature conservation method of banana tissue culture plantlet provenances |
CN105165606A (en) * | 2015-08-04 | 2015-12-23 | 江门市新会区林业科学研究所 | Preparation method of A.melanoxylon artificial seeds |
CN105746351A (en) * | 2016-03-09 | 2016-07-13 | 上海市农业生物基因中心 | Method for ultralow-temperature storage and renewal cultivation of wild rice stem tips |
-
2018
- 2018-11-30 CN CN201811453475.0A patent/CN109601378B/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1252685A (en) * | 1997-02-21 | 2000-05-10 | 莱拉·扎卡里亚·阿布德拉曼 | Sugar cane production |
EP1007629A2 (en) * | 1997-02-21 | 2000-06-14 | Layla Zakaria Abdelrahman | Sugar cane production |
CN102217537A (en) * | 2011-04-20 | 2011-10-19 | 中国科学院华南植物园 | Low-temperature conservation method of banana tissue culture plantlet provenances |
CN105165606A (en) * | 2015-08-04 | 2015-12-23 | 江门市新会区林业科学研究所 | Preparation method of A.melanoxylon artificial seeds |
CN105746351A (en) * | 2016-03-09 | 2016-07-13 | 上海市农业生物基因中心 | Method for ultralow-temperature storage and renewal cultivation of wild rice stem tips |
Non-Patent Citations (2)
Title |
---|
植物种质资源的保存——离体保存;张巧仙、范冬梅;《太原师范学院学报(自然科学版)》;20050331;第4卷(第1期);82-85 * |
甘蔗人工种子的研究;文颖等;《甘蔗糖业》;19951231(第1期);8-13 * |
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Publication number | Publication date |
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CN109601378A (en) | 2019-04-12 |
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GR01 | Patent grant | ||
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CP01 | Change in the name or title of a patent holder |
Address after: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee after: Institute of bioengineering, Guangdong Academy of Sciences Address before: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee before: GUANGDONG PROVINCIAL BIOENGINEERING INSTITUTE (GUANGZHOU SUGARCANE INDUSTRY RESEARCH INSTITUTE) Address after: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee after: Institute of biological and medical engineering, Guangdong Academy of Sciences Address before: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee before: Institute of bioengineering, Guangdong Academy of Sciences |
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CP01 | Change in the name or title of a patent holder | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230320 Address after: No. 10, shiliugang Road, Guangzhou, Guangdong 510000 Patentee after: Nanfan Seed Industry Research Institute Guangdong Academy of Sciences Address before: No.10, shiliugang Road, Haizhu District, Guangzhou City, Guangdong Province 510000 Patentee before: Institute of biological and medical engineering, Guangdong Academy of Sciences |
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TR01 | Transfer of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210824 |
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CF01 | Termination of patent right due to non-payment of annual fee |