CN109597266A - The camera installation and method of fluorescent material distribution in a kind of observation object - Google Patents
The camera installation and method of fluorescent material distribution in a kind of observation object Download PDFInfo
- Publication number
- CN109597266A CN109597266A CN201910083251.3A CN201910083251A CN109597266A CN 109597266 A CN109597266 A CN 109597266A CN 201910083251 A CN201910083251 A CN 201910083251A CN 109597266 A CN109597266 A CN 109597266A
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- China
- Prior art keywords
- fluorescent material
- camera
- material distribution
- observation object
- camera installation
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03B—APPARATUS OR ARRANGEMENTS FOR TAKING PHOTOGRAPHS OR FOR PROJECTING OR VIEWING THEM; APPARATUS OR ARRANGEMENTS EMPLOYING ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ACCESSORIES THEREFOR
- G03B17/00—Details of cameras or camera bodies; Accessories therefor
- G03B17/02—Bodies
- G03B17/12—Bodies with means for supporting objectives, supplementary lenses, filters, masks, or turrets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03B—APPARATUS OR ARRANGEMENTS FOR TAKING PHOTOGRAPHS OR FOR PROJECTING OR VIEWING THEM; APPARATUS OR ARRANGEMENTS EMPLOYING ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ACCESSORIES THEREFOR
- G03B15/00—Special procedures for taking photographs; Apparatus therefor
- G03B15/02—Illuminating scene
- G03B15/03—Combinations of cameras with lighting apparatus; Flash units
- G03B15/05—Combinations of cameras with electronic flash apparatus; Electronic flash units
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03B—APPARATUS OR ARRANGEMENTS FOR TAKING PHOTOGRAPHS OR FOR PROJECTING OR VIEWING THEM; APPARATUS OR ARRANGEMENTS EMPLOYING ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ACCESSORIES THEREFOR
- G03B19/00—Cameras
- G03B19/02—Still-picture cameras
- G03B19/12—Reflex cameras with single objective and a movable reflector or a partly-transmitting mirror
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The camera installation and method of fluorescent material distribution in a kind of observation object, suitable for observe the demand for looking into certain depth fluorescent material distribution in object.The invention patent is the technical solution adopted is that realize above-mentioned function by following structures and methods.Narrowband filter is mounted on the camera lens of digital camera, as photographic system.It installs optical filtering paper or colour filter additional in camera external flash lamp group, the intracorporal fluorescent material of object is excited by the illumination for lamp group of glistening, then synchronized and taken pictures with above-mentioned photographic system.The optical filtering paper or optical filter of particular color are installed additional using particular color LED light or on daylight type LED light surface, finding a view, focus and contour of object and surface fluorescence label illuminated in advance for digital camera, it is taken a picture with above-mentioned photographic system, is finally merged the lower image shot of two kinds of illuminations in advance again.The beneficial effect of the invention patent is at low cost, easy to use, for exciting the flash of light lamp group moment brightness of fluorescence big, launching efficiency is high, after the image of two kinds of lighting shootings is merged, it can get that spatial resolution is high, contrast is big, the position clearly high quality fluorescence distribution photo in object.
Description
Technical field
The invention patent relates to the camera installations and method of fluorescent material distribution in a kind of observation object, are by digital phase
Machine, narrowband filter and specific light source equipment combine what the observation fluorescent material to be formed was distributed in body surface certain depth
Photographic equipment and method.
Background technique
Using the spatial and temporal distributions information of certain specific molecular, cell or tissue in fluorescent material marker research organism,
It has a wide range of applications in medicine and biology.Above-mentioned technology and microscopes use, referred to as fluorescence microscope, can be to microcosmic
Biological structure studied, can also by special imaging system, to it is biological it is whole in the distribution of certain molecule grind
Study carefully, referred to as small animal living body imaging system or Molecular imaging techniques.
But the cost of above equipment is more expensive, is used to excite the form of light source of fluorescence single, and launching efficiency is not high,
Imaging system generallys use pseudo-colours computer reconstruction mode, and spatial resolution is not high, and picture quality is not ideal enough, to observed
The size etc. of object there are certain requirements, and practical ranges are by certain limitation.
To reduce equipment cost, facilitates application, improve fluorescence launching efficiency and image resolution ratio, the invention patent is with number
Camera add narrowband filter (being commonly called as optical filtering, optical filter) be image capturing system, using flash of light lamp group (multiple flash lamps) with
The technical solution that LED light adds optical filtering paper or optical filter to carry out fluorescence excitation and illuminate in advance, by realizing above-mentioned function with flowering structure:
Narrowband identical with specific fluorescent substance emission spectrum filter is mounted on camera gun, fluorescence is received
The fluorescence that matter issues；Install filter identical with observed fluorescent material excitation spectrum additional on the lamp cap of camera external flash lamp group
Machine-glazed paper or colour filter, the light source as excitation fluorescent material；Using identical specific with observed fluorescent material excitation spectrum
The LED light of color (wavelength), or install the optical filtering paper or optical filter of above-mentioned particular color additional on daylight type LED light surface, to containing
The profile of fluorescent material object and its fluorescent marker on surface carry out pre- illumination and pre- photograph.
The beneficial effect of the invention patent is that, using digital camera, installation shines with fluorescent material on the camera lens of camera
The identical narrowband filter of spectrum, to guarantee the fluorescence issued after fluorescent material is excited in primary recipient object.In number
The optical filtering paper or colour filter for installing particular color in the external flash lamp group of camera additional, form the exciting light of specific wavelength；It uses
Multiple flash lamps excite the fluorescent material in subject from different location and angle.Cost is relatively low for above-mentioned light source.
The moment brightness of flash lamp is big, and the master control flash lamp in lamp group of glistening is located on camera, is controlled by camera, subordinate flash lamp
Close to body surface or the side of object can be placed in or the back side is irradiated, wireless radio transmission can be passed through between master and slave flash lamp
Flash of light (wireless flash system) is synchronized, from different angles the intracorporal fluorescent material of excimer, is allowed to issue stronger glimmering
Light penetrates body surface, is acquired by camera installation, and the week fluorescent substance of certain depth in object is detected in realization.
Object is illuminated in advance with LED light, to facilitate the activities such as find a view, focus of camera, while can be to the general outline of object
It is taken a picture in advance with with mark point made by fluorescent material identical in object, so as in the formal photograph using Flashlamps exciting
Xiang Shi may be selected weaker light exposure, the imaging of irrelevant contents be reduced, to improve fluorescent material position and non-fluorescence substance position
Between image contrast.The image that can finally take a picture by pre- photograph and formally merges, and obtains existing fluorescent material in object
Interior spatial distribution, and have the full picture of contour of object and correlation margin.
The cost of the above camera installation part depends primarily on the cost of digital camera, usually the fluorescence imaging system than profession
It unites much lower, without carrying out quantitative analysis to fluorescence intensity, it is only necessary in the case where understanding its spatial distribution, can get higher
The fluorescent material spatial distribution image of spatial resolution (pixel depending on camera) and larger contrast.Special color LED light
Cost is slightly higher, and common daylight type LED light adds optical filtering paper or optical filter, and cost is relatively low.
The camera installation and method are at low cost, easy to use, are easy to universal, can utilize general photograph in different occasions
Phase equipment shoots the fluorescent material distributed image in the different distance visual field by zoom lens.The digital camera of current ten million pixel
Very cheap, sensitivity ISO is adjustable, and sensitivity is higher.It is shot in dark field, fluorescent spots and non-fluorescence position
Luminance difference is big, can get the photo more apparent than general living imaging system, contrast is bigger, facilitates look at fluorescent material and exist
The intracorporal fine distribution of object.
The invention patent is further illustrated with reference to the accompanying drawing.
Fig. 1 is the structural schematic diagram of the invention patent
1. object in figure, 2. fluorescent materials, 3. body surface fluorescent markers, 4. digital cameras, 5. narrowband filter, 6. is main
Control flash lamp, 7. subordinate flash lamps, 8. optical filtering paper or colour filter, 9.LED lamp, 10. optical filtering paper or optical filter.
Specific embodiment
In Fig. 1, have in object (1) fluorescent material (2), the mark that the useful identical fluorescent material in the surface of object (1) is done
Remember (3).Narrowband filter (5) are installed additional on the camera lens of digital camera (4), in master control flash lamp (6) and subordinate flash lamp (7)
Install optical filtering paper or colour filter (8) on lamp cap additional, when LED light (9) is special color or LED light (9) is daylight type, on it
Install optical filtering paper or optical filter (10) additional.
Master control flash lamp (6) and subordinate flash lamp (7) are by wireless radio transmission holding synchronization flash, on flash lamp (6) (7)
Optical filtering paper or colour filter (8) by flash lamp issue light color (wavelength) be converted into object (1) be observed fluorescent material
(2) excitation wavelength, excitation fluorescent material (2) issue fluorescence.Digital camera (4) is by narrowband filter (5) to fluorescent material
(2) fluorescence issued is formally taken a picture.
Light identical with observed fluorescent material excitation wavelength can be directly generated using special color LED light (8), or is made
With daylight type LED light (8), it is converted into and fluorescent marker (3) excitation wave appearance on object (1) by optical filtering paper or optical filter (9)
The illumination light of co-wavelength, fluorescent marker (3) to observed object (1) and thereon are illuminated in advance.Using digital camera (4) and
Narrowband filter (5), in the case where flash of light lamp group (6 7) is not glistened, by LED light (8) or LED light (8) plus optical filtering paper or
Under the illumination of optical filter (9), fluorescent marker (3) to object (1) and thereon is taken a picture in advance.It finally will can formally take a picture and in advance
The image of photograph enters fusion.
By apparatus above and method, it can be achieved that being observed using digital camera the distribution of fluorescent material in object.
Claims (10)
1. the camera installation and method of fluorescent material distribution in a kind of observation object, it is characterized in that: narrowband filter is mounted on
On camera gun, optical filtering paper or colour filter are installed additional on the lamp cap of camera external flash lamp group, to the intracorporal fluorescence of object
Substance is excited；Optical filtering paper or optical filtering are installed additional using the LED light with particular color (wavelength) or on daylight type LED light surface
Piece illuminates subject and surface fluorescence label in advance.
2. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that narrowband
The emission spectrum of fluorescent material, the emission spectrum of central wavelength and fluorescent material in the bandwidth covering observing object body of filter
Peak wavelength it is same or similar.
3. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that external
The optical filtering paper or colour filter that lamp group of glistening installs additional, the excitation wave of the wavelength and fluorescent material in observing object body of transmitted light wave
Length is same or similar.
4. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that specific
The LED light of color (wavelength), wavelength are same or similar with the excitation wavelength of fluorescent material in observing object body.
5. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that daylight
The optical filtering paper or optical filter that type LED light surface installs additional, the wave-length coverage of transmitted light wave and fluorescent material in observing object body
Excitation wavelength is same or similar.
6. the camera installation of fluorescent material distribution in observation object according to claim 1, which is characterized in that in flash of light lamp group
Master control flash lamp be located on camera, subordinate flash lamp is placed in close to around object, passes through nothing between master and slave flash lamp
Line electrical transmission control.
7. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that can make
It is individually illuminated with the external master control flash lamp of camera, or adds one or more subordinate flash syncs to join using master control flash lamp
Take a group photo bright.
8. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that photograph
Machine is the digital single-lens reflex camera mm professional camera special for having external flash lamp seat.
9. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that photograph
The built-in flash of machine can issue the light same or similar with fluorescent material excitation wavelength in observing object body.
10. the camera installation and method of fluorescent material distribution in observation object according to claim 1, which is characterized in that will
Photo using taking photos by using flashlights and the photo using the pre- lighting shooting of LED light carry out image co-registration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910083251.3A CN109597266A (en) | 2019-01-29 | 2019-01-29 | The camera installation and method of fluorescent material distribution in a kind of observation object |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910083251.3A CN109597266A (en) | 2019-01-29 | 2019-01-29 | The camera installation and method of fluorescent material distribution in a kind of observation object |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109597266A true CN109597266A (en) | 2019-04-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910083251.3A Pending CN109597266A (en) | 2019-01-29 | 2019-01-29 | The camera installation and method of fluorescent material distribution in a kind of observation object |
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| CN (1) | CN109597266A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755311A (en) * | 2021-11-09 | 2021-12-07 | 北京华牛世纪生物技术研究院 | Gene chip reader |
-
2019
- 2019-01-29 CN CN201910083251.3A patent/CN109597266A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113755311A (en) * | 2021-11-09 | 2021-12-07 | 北京华牛世纪生物技术研究院 | Gene chip reader |
| CN113755311B (en) * | 2021-11-09 | 2022-02-18 | 北京华牛世纪生物技术研究院 | Gene chip reader |
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| PB01 | Publication | ||
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Application publication date: 20190409 |