CN109596380B - A dissect fixing device that is used for early embryo sample of mouse to draw materials - Google Patents
A dissect fixing device that is used for early embryo sample of mouse to draw materials Download PDFInfo
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- CN109596380B CN109596380B CN201910070730.1A CN201910070730A CN109596380B CN 109596380 B CN109596380 B CN 109596380B CN 201910070730 A CN201910070730 A CN 201910070730A CN 109596380 B CN109596380 B CN 109596380B
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 40
- 239000000463 material Substances 0.000 title claims abstract description 8
- 230000000007 visual effect Effects 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 11
- 238000004804 winding Methods 0.000 claims description 19
- 239000011521 glass Substances 0.000 claims description 9
- 238000012806 monitoring device Methods 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims 1
- 238000005070 sampling Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 238000012545 processing Methods 0.000 abstract description 4
- 239000012188 paraffin wax Substances 0.000 description 6
- 239000011148 porous material Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 238000002224 dissection Methods 0.000 description 4
- 210000004291 uterus Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 210000000918 epididymis Anatomy 0.000 description 2
- 201000010063 epididymitis Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
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- 230000003321 amplification Effects 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000001531 micro-dissection Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Public Health (AREA)
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- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
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- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an anatomical fixing device for obtaining a mouse early embryo specimen, which relates to the technical field of medical experimental instruments and comprises a fixing frame, an ophthalmological forceps, a specimen orifice plate, a surface vessel base, a surface vessel and a visual amplifying device for amplifying and displaying a material obtaining process, wherein a specimen orifice plate cover is arranged on the specimen orifice plate. The invention can realize rapid, controllable and visual processing of early embryo specimens, improve the working efficiency of operators and reduce the loss of operation time; the device reduces the damage rate of the early embryo of the mouse in the process of processing the early embryo specimen, and realizes the saving of the early embryo of the mouse; realize good sample fixed effect.
Description
Technical Field
The invention relates to the technical field of medical experimental instruments, in particular to an anatomical fixing device for obtaining early-stage embryo specimens of a mouse.
Background
Mouse embryos are important tools for scientific research such as embryo development research, congenital malformation research and the like. The early embryo of the mouse is extremely small and difficult to obtain from the uterus, and particularly, the morphological research based on paraffin specimen has great obstacle, but the research of the embryo at the time is a key step for understanding the development of the mouse.
There are two ways to treat the paraffin wax specimen of the early embryo of the mouse before fixing, one way is to dissect the embryo in the uterus completely and then fix it (from handbook of experimental manipulation of embryo of mouse (third edition)), which is easy to cause: the microdissection is long in time, so that protein or RNA is easily degraded, and the immunohistochemistry or in-situ hybridization detection effect is influenced; secondly, the embryo of the early mouse is extremely small, and is easy to lose in the subsequent processing links of gradient alcohol dehydration, xylene transparence, paraffin embedding and the like; and thirdly, the embryonic tissue of the early mouse is very delicate and easy to damage due to the collision of dissecting instruments or test tube bottle walls. Another treatment is to immobilize the embryo directly with the surrounding uterine wall without dissecting the uterus, which easily leads to: firstly, because of the wrapping protection of the uterine wall, fixing liquid is not easy to permeate, and the fixing effect of the embryo specimen is not good; secondly, the embryo is in the rotating process in the early development stage, the embryo head and tail directions cannot be distinguished due to the wrapping and shielding of the uterine wall, the embryo embedding and slicing angles cannot be guaranteed, the requirement can be met only by repeatedly and blindly cutting dozens of specimens, and the great waste of time, energy, animals and the like is caused.
In conclusion, how to effectively change the selection of two difficult ways of the fixation pretreatment of the early embryo paraffin specimen of the mouse, solve the technical problems of the prior art that the early embryo specimen is treated with low efficiency and the specimen fixation effect is not ideal, and the like, is a problem which needs to be solved by the technicians in the field at present.
Disclosure of Invention
In order to solve the technical problem, the invention provides an anatomical fixing device for obtaining a mouse early embryo paraffin specimen.
The utility model provides an anatomy fixing device that is used for early embryo sample of mouse to draw materials, includes the mount, still includes mount, ophthalmology tweezers, sample orifice plate, surface ware base, surface ware, still is provided with the visual amplification device that the process of drawing materials enlargies the demonstration.
Furthermore, the fixed frame comprises a fixed base, an adjusting frame capable of adjusting the height and the horizontal direction, and a cutting knife clamping part is arranged at the end part of the adjusting frame far away from the fixed base;
clamping grooves are respectively formed in the cutting knife clamping part and the adjusting frame, the clamping grooves are connected with the cutting knife operating handle in a clamping mode, and a cutting blade is arranged at one side end of the cutting knife operating handle;
a specimen orifice plate cover is arranged on the specimen orifice plate;
the surface dish is arranged on the surface dish base, and an illuminating lamp for transmitting illumination of the surface dish is arranged in the surface dish base.
Furthermore, the outer surface of the adjusting frame is provided with a first threading hole, a hollow part is arranged in the adjusting frame, an ophthalmic forceps connecting wire is connected to the ophthalmic forceps, one end of the ophthalmic forceps connecting wire is fixedly connected with the adjusting frame in the hollow part, and the other end of the ophthalmic forceps connecting wire penetrates through the first threading hole to be connected with the ophthalmic forceps.
Furthermore, an operating handle connecting wire is connected to the cutting knife operating handle, a distribution groove which is connected with the operating handle connecting wire and can be distributed is formed in the outer surface of the adjusting frame, a second threading hole is formed in the outer surface of the adjusting frame, one end of the operating handle connecting wire is fixedly connected with the adjusting frame in the hollow portion, and the other end of the operating handle connecting wire penetrates through the second threading hole to be connected with the cutting knife operating handle.
Further, the visual amplifying device is arranged on the adjusting frame, and the visual amplifying device
Is a magnifying glass.
Furthermore, the visual amplifying device is connected with a real-time online monitoring device.
Further, the real-time online monitoring device is arranged on the inner surface of the cutting knife clamping part.
Furthermore, a connecting wire winding device for winding the ophthalmic forceps connecting wire and the operating handle connecting wire is arranged on the adjusting frame;
further, the ophthalmic forceps can be free of the ophthalmic forceps connecting line, and when the ophthalmic forceps is free of the ophthalmic forceps connecting line, the ophthalmic forceps are hung on the first threading hole.
Further, the connecting wire winding device is arranged in the hollow part in the adjusting frame.
Further, the sample hole plate is provided with sample holes with different hole diameters.
Advantageous effects
1. The early embryo specimen is quickly, controllably and visually processed, the working efficiency of operators is improved, and the operation time loss is reduced;
2. the device reduces the damage rate of the early embryo of the mouse in the process of processing the early embryo specimen, and realizes the saving of the early embryo of the mouse;
3. realize good sample fixed effect.
Drawings
FIG. 1 is a simplified schematic structural diagram of an anatomical-fixing device for obtaining a specimen of an early mouse embryo according to the present embodiment;
FIG. 2 is a schematic diagram of the real-time online monitoring device in the present embodiment;
fig. 3 is a schematic diagram of the structure of the connecting wire take-up device in this embodiment.
The reference numbers in the drawings are:
1. a fixed base; 2. an adjusting bracket; 3. a second threading hole; 4. a first threading hole;
5. a cutter operating handle; 6. a cloth arranging groove; 7. a visual magnification device; 8. a cutting knife clamping part;
9. a clamping groove; 10. a cutting blade; 11. a watch glass; 12. a watch glass base;
13. ophthalmic forceps; 14. a specimen hole; 15. a specimen well plate; 16. a connecting wire winding wheel;
17. and (7) winding the shaft.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
As shown in fig. 1, an anatomical fixing device for obtaining a sample of an early embryo of a mouse comprises a fixing frame, an ophthalmic forceps 13, a sample pore plate 15, a surface dish base 12, a surface dish 11, and a visual amplifying device 7 for amplifying and displaying a material obtaining process;
the fixed frame comprises a fixed base 1, an adjusting frame 2 with adjustable height and in the horizontal direction, and a cutting knife clamping part 8 is arranged at the end part of the adjusting frame 2 far away from the fixed base 1;
clamping grooves are respectively formed in the cutting knife clamping part 8 and the adjusting frame, the clamping grooves are connected with the cutting knife operating handle 5 in a clamping mode, and a cutting blade 10 is arranged at one side end part of the cutting knife operating handle 5;
a specimen orifice plate cover is arranged on the specimen orifice plate 15;
the watch glass 11 is arranged on the watch glass base 12, and an illuminating lamp for transmitting illumination of the watch glass 11 is arranged in the watch glass base 12.
The outer surface of the adjusting frame 2 is provided with a first threading hole 4, a hollow part is arranged inside the adjusting frame 2, an ophthalmic forceps connecting wire is connected to the ophthalmic forceps 13, one end of the ophthalmic forceps connecting wire is fixedly connected with the adjusting frame 2 in the hollow part, and the other end of the ophthalmic forceps connecting wire penetrates through the first threading hole 4 to be connected with the ophthalmic forceps 13.
The cutting knife operation handle is characterized in that an operation handle connecting wire is connected onto the cutting knife operation handle 5, a distribution groove 6 which can be used for distributing the operation handle connecting wire is formed in the outer surface of the adjusting frame 2, a second threading hole 3 is further formed in the outer surface of the adjusting frame 2, one end of the operation handle connecting wire is fixedly connected with the adjusting frame 2 in the hollow portion, and the other end of the operation handle connecting wire penetrates through the second threading hole 3 to be connected with the cutting knife operation handle 5.
As shown in fig. 1-2, the visual magnifying device 7 is disposed on the adjusting frame 2, and the visual magnifying device 7 is a magnifying glass. The magnifying lens has a magnification of 4 times or 10 times, and the visual magnifying device 7 can also be an optical magnifying lens or an electron microscope. The visual magnification means 7 may make the dissection process clearer.
The visual amplifying device 7 is connected with a real-time online monitoring device, and the real-time online monitoring device can realize that other people remotely participate in the dissection process in real time and give suggestions to the dissection process, can store the influence of the dissection process and is used for daily teaching.
The real-time online monitoring device is arranged on the inner surface of the cutting knife clamping part 8, and the real-time online monitoring device can be a product in the prior art.
As shown in fig. 3, a connecting wire winding device for winding the ophthalmic forceps connecting wire and the operating handle connecting wire is arranged on the adjusting frame 2, the connecting wire winding device comprises a connecting wire winding wheel 16 for applying force in the same direction, a winding shaft 17 is arranged at the axis of the connecting wire winding wheel 16, and the shaft ends of the two sides of the winding shaft 17 are respectively connected with the inner side wall of the adjusting frame 2. Because the ophthalmic forceps connecting wire and the circuit of operation handle connecting wire are more longer, connecting wire coiling mechanism can prevent to dissect each connecting wire of in-process and take place the winding, improves and dissects efficiency.
When the ophthalmological forceps 13 and/or the cutting knife operation handle 5 are in a non-working state, the connecting wire winding wheel 16 applies force in a clockwise direction and is static; when the ophthalmological forceps 13 and/or the cutting knife operation handle 5 are/is in a working state, the connecting wire winding wheel 16 is stretched outwards, and rotates in the anticlockwise direction; when the operation state of the ophthalmological forceps 13 and/or the cutting knife operation handle 5 is just finished, the connecting wire winding wheel 16 rotates clockwise until the ophthalmological forceps connecting wire and the operation handle connecting wire are completely retracted and then are static.
The connecting wire winding device is arranged in the hollow part in the adjusting frame 2.
The ophthalmological tweezers 13 can also be free of the ophthalmological tweezers connecting wire, and when the ophthalmological tweezers 13 is free of the ophthalmological tweezers connecting wire, the ophthalmological tweezers 13 are hung on the first threading hole 4.
The sample hole plate 15 is provided with sample holes 14 having different pore sizes. Wherein, the aperture size of the sample hole 14 of each horizontal row on the sample hole plate 15 is the same, and the aperture size of the sample hole 14 of the adjacent horizontal row is different; the sample holes 14 in each vertical row on the sample well plate 15 have the same pore size, and the sample holes 14 in adjacent vertical rows have different pore sizes.
The main steps of using the materials of the invention are as follows:
separating the uterus, cutting the embryo bags one by one, immersing the embryo bags into the fixing liquid, and downwards placing the mesenteric end of the embryo bags into the specimen holes 14 with proper sizes;
1/3 uterine wall is cut off with cutting blade 10, and the epididymis is peeled off gently with ophthalmic forceps 13 until the epididymis is visible;
the pore plate cover covering the sample pore plate 15 is continuously fixed, dehydrated, transparent and waxed;
and opening the orifice plate cover, taking out the embryo and carrying out paraffin embedding. The embedding angle is adjusted according to the position of the embryo during embedding.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. An anatomical fixing device for obtaining mouse early embryo specimen comprises a fixing frame
Is characterized by also comprising ophthalmological tweezers, a specimen orifice plate, a surface vessel base, a surface vessel and a visual amplifying device for amplifying and displaying the material drawing process, wherein the fixing frame comprises a fixing base, an adjusting frame with adjustable height and horizontal direction, and a cutting knife clamping part is arranged at the end part of the adjusting frame far away from the fixing base, the cutting knife clamping part and the adjusting frame are respectively provided with a clamping groove, the clamping groove is clamped with the cutting knife operating handle, one side end part of the cutting knife operating handle is provided with a cutting blade, the outer surface of the adjusting frame is provided with a first threading hole, a hollow part is arranged inside the adjusting frame, the ophthalmological tweezers are connected with ophthalmological tweezers connecting wires, one end of the ophthalmological tweezers connecting wire is fixedly connected with the adjusting frame in the hollow part, and the other end of the ophthalmological tweezers connecting wire passes through the first threading hole to be connected with the ophthalmological tweezers, the cutting knife is characterized in that an operating handle connecting wire is connected to the cutting knife operating handle, a distributing groove capable of distributing the operating handle connecting wire is formed in the outer surface of the adjusting frame, a second threading hole is formed in the outer surface of the adjusting frame, one end of the operating handle connecting wire is arranged in the hollow portion and fixedly connected with the adjusting frame, and the other end of the operating handle connecting wire penetrates through the second threading hole and is connected with the cutting knife operating handle.
2. The anatomical-fixation device for obtaining a specimen of an early mouse embryo according to claim 1, wherein,
a specimen orifice plate cover is arranged on the specimen orifice plate;
the surface dish is arranged on the surface dish base, and an illuminating lamp for transmitting illumination of the surface dish is arranged in the surface dish base.
3. The anatomical fixing device for obtaining a specimen of an early mouse embryo according to claim 1, wherein the visual magnifying device is disposed on the adjusting bracket, and the visual magnifying device is a magnifying glass.
4. The anatomical fixing device according to claim 3, wherein the visual amplifying device is connected with a real-time on-line monitoring device.
5. An anatomical fixing device as claimed in claim 4, wherein the real-time on-line monitoring device is disposed on the inner surface of the cutting knife holding portion.
6. The anatomical fixing device for obtaining a mouse early embryo specimen according to claim 1, wherein a connecting wire winding device for winding the ophthalmic forceps connecting wire and the operating handle connecting wire respectively is arranged on the adjusting bracket;
the ophthalmological tweezers can be free of the ophthalmological tweezers connecting wire, and when the ophthalmological tweezers are free of the ophthalmological tweezers connecting wire
During threading, the ophthalmological forceps are hung on the first threading hole.
7. The anatomical fixing device for obtaining a specimen of an early mouse embryo according to claim 6, wherein the connecting wire rolling device is disposed in the hollow portion of the adjusting rack.
8. An anatomical fixing device for use in the sampling of mouse early embryo according to claim 1, wherein the sample well plate is provided with sample wells with different sizes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910070730.1A CN109596380B (en) | 2019-01-25 | 2019-01-25 | A dissect fixing device that is used for early embryo sample of mouse to draw materials |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910070730.1A CN109596380B (en) | 2019-01-25 | 2019-01-25 | A dissect fixing device that is used for early embryo sample of mouse to draw materials |
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| CN109596380A CN109596380A (en) | 2019-04-09 |
| CN109596380B true CN109596380B (en) | 2021-08-13 |
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| CN110833466B (en) * | 2019-11-27 | 2021-11-23 | 佛山科学技术学院 | Poultry embryo dissection platform |
| CN114699211B (en) * | 2022-03-21 | 2024-08-20 | 浙江省农业科学院 | Early embryo locator for microinjection of Chinese soft-shelled turtles and embryo locating method |
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| US8980568B2 (en) * | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
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