CN109593690B - A kind of industrial process of beneficial bacteria of intestinal tract preparation - Google Patents
A kind of industrial process of beneficial bacteria of intestinal tract preparation Download PDFInfo
- Publication number
- CN109593690B CN109593690B CN201910114816.XA CN201910114816A CN109593690B CN 109593690 B CN109593690 B CN 109593690B CN 201910114816 A CN201910114816 A CN 201910114816A CN 109593690 B CN109593690 B CN 109593690B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- cultured
- fermented
- industrial process
- intestinal tract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 82
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 230000009286 beneficial effect Effects 0.000 title claims abstract description 34
- 210000001035 gastrointestinal tract Anatomy 0.000 title claims abstract description 32
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 31
- 238000000855 fermentation Methods 0.000 claims abstract description 73
- 230000004151 fermentation Effects 0.000 claims abstract description 73
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 239000003223 protective agent Substances 0.000 claims abstract description 22
- 229920002472 Starch Polymers 0.000 claims abstract description 21
- 239000008107 starch Substances 0.000 claims abstract description 21
- 239000011248 coating agent Substances 0.000 claims abstract description 15
- 238000000576 coating method Methods 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000012153 distilled water Substances 0.000 claims abstract description 12
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 10
- 241000186660 Lactobacillus Species 0.000 claims abstract description 9
- 238000001514 detection method Methods 0.000 claims abstract description 9
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 9
- 241000186016 Bifidobacterium bifidum Species 0.000 claims abstract description 8
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 8
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 8
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 8
- 229940002008 bifidobacterium bifidum Drugs 0.000 claims abstract description 8
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 8
- 238000001035 drying Methods 0.000 claims abstract description 7
- 238000001291 vacuum drying Methods 0.000 claims abstract description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 28
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 21
- 241000319304 [Brevibacterium] flavum Species 0.000 claims description 17
- 239000002609 medium Substances 0.000 claims description 17
- 239000000843 powder Substances 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 15
- 239000008103 glucose Substances 0.000 claims description 14
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 14
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 10
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 10
- 239000011814 protection agent Substances 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 235000012241 calcium silicate Nutrition 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 244000068988 Glycine max Species 0.000 claims description 8
- 235000010469 Glycine max Nutrition 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- 230000002779 inactivation Effects 0.000 claims description 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 5
- 108010019160 Pancreatin Proteins 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 239000005018 casein Substances 0.000 claims description 5
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 5
- 235000021240 caseins Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 239000001963 growth medium Substances 0.000 claims description 5
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 229940055695 pancreatin Drugs 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 5
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 5
- 239000001509 sodium citrate Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 235000015193 tomato juice Nutrition 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- VLSOAXRVHARBEQ-UHFFFAOYSA-N [4-fluoro-2-(hydroxymethyl)phenyl]methanol Chemical compound OCC1=CC=C(F)C=C1CO VLSOAXRVHARBEQ-UHFFFAOYSA-N 0.000 claims description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 2
- 238000005469 granulation Methods 0.000 claims description 2
- 230000003179 granulation Effects 0.000 claims description 2
- 239000011574 phosphorus Substances 0.000 claims description 2
- 229910052698 phosphorus Inorganic materials 0.000 claims description 2
- ZGBSOTLWHZQNLH-UHFFFAOYSA-N [Mg].S(O)(O)(=O)=O Chemical compound [Mg].S(O)(O)(=O)=O ZGBSOTLWHZQNLH-UHFFFAOYSA-N 0.000 claims 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- 235000019698 starch Nutrition 0.000 claims 1
- 239000006041 probiotic Substances 0.000 abstract description 20
- 235000018291 probiotics Nutrition 0.000 abstract description 20
- 238000000034 method Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 4
- 238000009472 formulation Methods 0.000 abstract description 3
- 230000000284 resting effect Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 6
- 230000036541 health Effects 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 239000000654 additive Substances 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N D-Maltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 235000013406 prebiotics Nutrition 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186012 Bifidobacterium breve Species 0.000 description 1
- 241001468229 Bifidobacterium thermophilum Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 1
- 241001561398 Lactobacillus jensenii Species 0.000 description 1
- 241000186605 Lactobacillus paracasei Species 0.000 description 1
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000007661 gastrointestinal function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of industrial processes of beneficial bacteria of intestinal tract preparation, belong to microorganism formulation technical field.The production method is the following steps are included: (1) obtains fermentation culture respectively by Lactobacillus casei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus delbruockii subspecies bulgaricus progress fermented and cultured；(2) streptococcus thermophilus is subjected to fermented and cultured, adds porous-starch into fermentation liquid before fermentation ends, obtains fermentation culture；(3) fermentation liquid of collection step (1) and the resulting each strain of step (2) obtains the wet bacterium mud of activity, adds distilled water to be uniformly mixed with protective agent wet bacterium mud；(4) it is pelletized by granulator, spheronizator, drying machine, seed-coating machine, is round as a ball, low-temperature vacuum drying, coating, obtaining beneficial bacteria of intestinal tract preparation after detection is qualified.It is able to ascend the quantity of viable bacteria in probiotics preparation using method of the invention, extends the resting period of probiotics preparation, reduces production cost.
Description
Technical field
The invention belongs to microorganism formulation technical fields, and in particular to a kind of industrial process of beneficial bacteria of intestinal tract.
Background technique
With the variation of socio-economic development and natural environment, rhythm of life is accelerated, and various pressure are also comed one after another, people
There is the problem of various healths aspects.And probiotics and people carry out the connection of health own profound.It is prebiotic
Bacterium is a kind of active microorganism beneficial to host, is to be colonized in human body intestinal canal, in reproductive system, can generate definite health efficacy
So as to improve the active beneficial microorganism general name of host's microecological balance, performance beneficial effect.Probiotics is roughly divided into three classes,
Including lactobacillus (such as lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, Raman lactobacillus), Bifidobacterium is (such as length
Bifidobacterium, bifidobacterium breve, oval Bifidobacterium, bifidobacterium thermophilum etc.), Gram positive cocci (such as streptococcus fecalis, cream
Coccus, intermediary streptococcus etc.), in addition, there are also the scopes that some yeast can also be included into probiotics.
With going deep into for probiotics and human health relationship research, it has been found that probiotics integrates with following several sides
The effect in face: (1) it improves immunity: generating a large amount of immunoglobulin, activate the immunocyte of body, so that external bacterium is difficult to
It retains in vivo, reaches immunoregulation effect；(2) improve gastrointestinal function: forming protective barrier in gut mucosal surface, resisted
Evil bacterium destroys the invasion of intestinal mucosa, causes the environment for being unfavorable for harmful bacteria growth, inhibits growth, the breeding of harmful bacteria；(3)
Trophism: fermentation generates lactic acid and acetic acid, can improve the utilization rate of calcium, phosphorus, iron, promotes the digestion and suction of related nutritional substance
It receives；(4) lead to intestines profit just: can produce a large amount of acidic materials in the intestine, stimulate intestines peristalsis, play the effect etc. of defaecation.Enteron aisle is micro-
The research and development of ecological agent product can carry out positive promote meaning to the health care belt of the mankind, with important economic value and
Community health's meaning.
Chinese patent announces CN108783462A and discloses a kind of industrial process of beneficial bacteria of intestinal tract preparation, including
(1) Lactobacillus casei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus acidophilus, streptococcus thermophilus, Lactobacillus delbrueckii are protected
Leah subspecies etc. are added to carry out mass propgation respectively with fermentor, (2) obtain wet bacterium mud by centrifugation and sterile water washing, by wet bacterium
Mud is uniformly mixed with special protective preparation by 1:1, and (3) pelletized wet bacterium mud and protectant mixture, round as a ball, cryogenic vacuum
Dry, coating, is made beneficial bacteria of intestinal tract preparation.The invention provide condition of culture under, probiotics can high-density growth breeding,
It collects obtained wet bacterium mud to be not required to be freeze-dried, vacuum and low temperature rapid draing is directly carried out with a kind of special protective preparation and can keep
Thallus large number of viable saves the energy, reduces production cost.But this method needs to add a large amount of protection in use
Agent reduces the relative amount of viable count in probiotics preparation.Chinese patent announcement CN102614225A discloses a kind of compound
Probiotics viable bacteria preparation and its industrialized preparing process, the complex probiotics viable bacteria preparation are double by lactobacillus acidophilus A-10, youth
Discrimination bacillus B-13, auxiliary protection carrier mix, and wherein compound probiotic bacterium powder is 2.0%-30%, and auxiliary protection carrier is
70-98%, although the invention improves the number of viable and survival rate of probiotics to a certain extent, still exist auxiliary
The problem for helping protection carrier dosage big.
So being badly in need of a kind of industrial process for the probiotics that Viable detection is high, protective agent additive amount is few at present.
Summary of the invention
The present invention provides a kind of industrial processes of beneficial bacteria of intestinal tract preparation, comprising the following steps: (1) will do respectively
Lactobacillus paracasei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus delbruockii subspecies bulgaricus are inoculated into different fermented and cultureds
Fermented and cultured is carried out in base, obtains fermentation culture；(2) streptococcus thermophilus is inoculated into fermentation medium and carries out fermentation training
It supports, adds porous-starch before fermentation ends into fermentation liquid, obtain fermentation culture；(3) collection step (1) and step (2) institute
The fermentation liquid of each strain obtained obtains the wet bacterium mud of activity, adds distilled water to be uniformly mixed with protective agent wet bacterium mud；(4) by granulation
Machine, spheronizator, drying machine, seed-coating machine pelletized, be round as a ball, low-temperature vacuum drying, coating, obtains enteron aisle after detection is qualified
Probiotics preparation.It is able to ascend the quantity of viable bacteria in probiotics preparation using method of the invention, extends depositing for probiotics preparation
It puts the time, reduces production cost.
The present invention provides a kind of industrial process of beneficial bacteria of intestinal tract preparation, this method is in lower protective agent additive amount
On the basis of, it can guarantee higher Viable detection, significantly improve the content of viable bacteria in every gram of finished product.
The present invention provides a kind of industrial processes of beneficial bacteria of intestinal tract preparation, the described method comprises the following steps:
(1) respectively by Lactobacillus casei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus delbruockii subspecies bulgaricus
It is inoculated into different fermentation mediums and carries out fermented and cultured, when viable count is up to standard in fermented and cultured, terminates fermented and cultured, obtain
To fermentation culture；
(2) streptococcus thermophilus is inoculated into fermentation medium and carries out fermented and cultured, added before fermentation ends into fermentation liquid
Add porous-starch, obtains fermentation culture；
(3) fermentation liquid of collection step (1) and the resulting each strain of step (2) and mixing, are centrifuged and are lived after washing
Property wet bacterium mud, add distilled water to be uniformly mixed with protective agent wet bacterium mud, obtain bacterium mud protection agent composition, wherein wet bacterium mud: protecting
Protect agent: the mass ratio of distilled water is 1:0.5-0.8:0.4-0.8；
(4) the obtained bacterium mud protection agent composition of step (3) is successively passed through into granulator, spheronizator, drying machine, coating
Machine pelletized, is round as a ball, low-temperature vacuum drying, coating, obtains beneficial bacteria of intestinal tract preparation after detection is qualified.
Fermentative medium formula in the step (1) are as follows: glucose 5.0-15.0g, pancreatin hydrolysis casein 2.0-
15.0g, yeast extract 1.5-5.5g, sodium citrate 1.0-5.0g, dipotassium hydrogen phosphate 0.2-2.5g, magnesium sulfate 0.1-0.75g, L- half
Cystine hydrochloride 0.001-0.1g, Tomato juice 2.0-25g, Tween-20 0.5-2.0mL, Jia Shui are settled to 1000mL, pH
6.0-7.0,105 DEG C sterilize 15 minutes.
The step (1) when in fermentation liquid viable count reach 3-4.5 × 109When cfu/mL, terminate fermented and cultured.
Fermentation medium in the step (2) are as follows: glucose 95-98g/L, ammonium sulfate 7-10g/L, peptone 95-99g/
L, ammonium nitrate 4-8g/L, potassium dihydrogen phosphate 3-4g/L, magnesium sulfate 1-2g/L, pH 6.8-7.1,105 DEG C sterilize 15 minutes.
The step (2) when in fermentation liquid viable count reach 2-3 × 109When cfu/mL, porous-starch is added, continues to ferment
Culture 1-5 hours.
The step (2) adds porous-starch, makes it in initial content 10-20g/L.
Cultivation temperature in step (1) and step (2) is 30 DEG C -45 DEG C.
Protective agent in the step (3) includes each substance of following mass parts: 1-2 parts of glycerol, 2-3 parts of oligomeric different malt
Sugar, 2-4.5 parts of oligofructose, 0.1-2.5 parts of soybean peptides, 2-8 parts of calcium silicates.
In some preferred technical solutions, the brevibacterium flavum in the protective agent also containing the inactivation of 1-2 mass parts is sent out
Zymotic fluid.
The preparation method of the brevibacterium flavum fermentation liquid of the inactivation is that culture medium includes: glucose 20-40g/L, yeast
Powder 2-8g/L, ammonium sulfate 2-8g/L, Dried Corn Steep Liquor Powder 15-25g/L, potassium dihydrogen phosphate 0.5-3g/L, magnesium sulfate 0.8-1.5g/L；
PH is 6.9-7.2；Inoculum concentration is calculated as 0.1g/L with brevibacterium flavum bacterium powder；30 DEG C -45 DEG C of cultures 3-8 hours；High-temperature sterilization.
Compared with prior art, the invention has the benefit that
(1) present invention is reduced by before fermentation ends, adding porous-starch into the fermentation medium of streptococcus thermophilus
The addition of porous-starch in wet bacterium mud, and then increase the quantity of viable bacteria in every gram of product.
(2) fermentation liquid of the invention by adding a certain amount of brevibacterium flavum through inactivating in beneficial bacteria of intestinal tract, is improved
The survival rate of probiotics in final product.
(3) present invention is by adding porous-starch in the streptococcus thermophilus fermentation later period simultaneously and adding yellow in wet bacterium mud
Quarter butt fermented liquid extends the resting period of beneficial bacteria of intestinal tract preparation.
(4) present invention is it has unexpectedly been discovered that it is prebiotic that calcium silicates can increase every gram of enteron aisle on the basis of inventive formulation
Number of viable in bacteria preparation.
Specific embodiment
Unless otherwise defined, all technical and scientific terms used herein have and the technical field of the invention
The normally understood identical meaning of those of ordinary skill.In the examples where no specific technique or condition is specified, according in the art
Conventional technical means carries out.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of embodiment 1
(1) respectively by Lactobacillus casei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus delbruockii subspecies bulgaricus
It is inoculated into different fermentation mediums and carries out fermented and cultured, when viable count reaches 4.5 × 10 in fermented and cultured9When cfu/mL,
Terminate fermented and cultured, obtain fermentation culture, wherein cultivation temperature is 30 DEG C DEG C, and the formula of fermentation medium is equal are as follows: glucose
15.0g, pancreatin hydrolysis casein 2.0g, yeast extract 5.5g, sodium citrate 1.0g, dipotassium hydrogen phosphate 2.5g, magnesium sulfate 0.1g, L-
Cysteine hydrochloride 0.1g, Tomato juice 2.0g, Tween-20 2.0mL, Jia Shui are settled to 6.0,105 DEG C of 1000mL, pH and go out
Bacterium 15 minutes；
(2) streptococcus thermophilus is inoculated into fermentation medium and carries out fermented and cultured, when in fermentation liquid viable count reach 3 ×
109When cfu/mL, porous-starch is added into fermentation liquid, makes initial content 10g/L of the porous-starch in fermented and cultured, after
Continuous fermented and cultured 5 hours, fermentation culture is obtained, cultivation temperature is 30 DEG C, fermentation medium used are as follows: glucose 98g/L, sulphur
Sour ammonium 7g/L, peptone 99g/L, ammonium nitrate 4g/L, potassium dihydrogen phosphate 4g/L, 7.1,105 DEG C of magnesium sulfate 1g/L, pH sterilizings 15
Minute；
(3) fermentation liquid of collection step (1) and the resulting each strain of step (2) and mixing, are centrifuged and are lived after washing
Property wet bacterium mud, add distilled water to be uniformly mixed with protective agent wet bacterium mud, obtain bacterium mud protection agent composition, wherein wet bacterium mud: protecting
Protect agent: the mass ratio of distilled water be 1:0.5:0.8, the protective agent include following mass parts each substance: 1 part of glycerol, 3 parts it is low
Polyisomaltose, 2 parts of oligofructose, 2.5 parts of soybean peptides, 2 parts of calcium silicates；
(4) the obtained bacterium mud protection agent composition of step (3) is successively passed through into granulator, spheronizator, drying machine, coating
Machine pelletized, is round as a ball, low-temperature vacuum drying, coating, obtains beneficial bacteria of intestinal tract preparation after detection is qualified.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of embodiment 2
(1) respectively by Lactobacillus casei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus delbruockii subspecies bulgaricus
It is inoculated into different fermentation mediums and carries out fermented and cultured, when viable count reaches 3 × 10 in fermented and cultured9When cfu/mL, knot
Beam fermented and cultured, obtains fermentation culture, wherein cultivation temperature is 45 DEG C, and the formula of fermentation medium is equal are as follows: glucose
5.0g, pancreatin hydrolysis casein 15.0g, yeast extract 1.5g, sodium citrate 5.0g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.75g,
L-cysteine hydrochloride 0.001g, Tomato juice 25g, Tween-20 0.5mL, Jia Shui are settled to 1000mL, pH 7.0,105
DEG C sterilizing 15 minutes；
(2) streptococcus thermophilus is inoculated into fermentation medium and carries out fermented and cultured, when in fermentation liquid viable count reach 2 ×
109When cfu/mL, porous-starch is added into fermentation liquid, makes initial content 20g/L of the porous-starch in fermented and cultured, after
Continuous fermented and cultured 1 hour, fermentation culture is obtained, cultivation temperature is 45 DEG C, fermentation medium used are as follows: glucose 95g/L, sulphur
Sour ammonium 10g/L, peptone 95g/L, ammonium nitrate 8g/L, potassium dihydrogen phosphate 3g/L, 6.8,105 DEG C of magnesium sulfate 2g/L, pH sterilizings 15
Minute；
(3) fermentation liquid of collection step (1) and the resulting each strain of step (2) and mixing, are centrifuged and are lived after washing
Property wet bacterium mud, add distilled water to be uniformly mixed with protective agent wet bacterium mud, obtain bacterium mud protection agent composition, wherein wet bacterium mud: protecting
Protect agent: the mass ratio of distilled water be 1:0.8:0.4, the protective agent include following mass parts each substance: 2 parts of glycerol, 2 parts it is low
Polyisomaltose, 4.5 parts of oligofructose, 0.1 part of soybean peptide, 8 parts of calcium silicates；
(4) the obtained bacterium mud protection agent composition of step (3) is successively passed through into granulator, spheronizator, drying machine, coating
Machine pelletized, is round as a ball, low-temperature vacuum drying, coating, obtains beneficial bacteria of intestinal tract preparation after detection is qualified.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of embodiment 3
(1) respectively by Lactobacillus casei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus delbruockii subspecies bulgaricus
It is inoculated into different fermentation mediums and carries out fermented and cultured, when viable count reaches 3.5 × 10 in fermented and cultured9When cfu/mL,
Terminate fermented and cultured, obtain fermentation culture, wherein cultivation temperature is 40 DEG C, and the formula of fermentation medium is equal are as follows: glucose
10.0g, pancreatin hydrolysis casein 8.0g, yeast extract 4.2g, sodium citrate 4.0g, dipotassium hydrogen phosphate 2.1g, magnesium sulfate 0.5g, L-
Cysteine hydrochloride 0.006g, Tomato juice 15g, Tween-20 1.0mL, Jia Shui are settled to 6.5,105 DEG C of 1000mL, pH
Sterilizing 15 minutes；
(2) streptococcus thermophilus is inoculated into fermentation medium and carries out fermented and cultured, when viable count reaches 2.3 in fermentation liquid
×109When cfu/mL, porous-starch is added into fermentation liquid, makes initial content 15g/L of the porous-starch in fermented and cultured,
Continue fermented and cultured 3 hours, obtain fermentation culture, cultivation temperature is 40 DEG C, fermentation medium used are as follows: glucose 97g/L,
Ammonium sulfate 8g/L, peptone 97g/L, ammonium nitrate 7g/L, potassium dihydrogen phosphate 3.5g/L, 7.0,105 DEG C of magnesium sulfate 1.2g/L, pH
Sterilizing 15 minutes；
(3) fermentation liquid of collection step (1) and the resulting each strain of step (2) and mixing, are centrifuged and are lived after washing
Property wet bacterium mud, add distilled water to be uniformly mixed with protective agent wet bacterium mud, obtain bacterium mud protection agent composition, wherein wet bacterium mud: protecting
Protect agent: the mass ratio of distilled water is 1:0.6:0.7, and the protective agent includes each substance of following mass parts: 1.5 parts of glycerol, 2.1
Part oligoisomaltose, 3.0 parts of oligofructose, 1.2 parts of soybean peptides, 6 parts of calcium silicates；
(4) the obtained bacterium mud protection agent composition of step (3) is successively passed through into granulator, spheronizator, drying machine, coating
Machine pelletized, is round as a ball, low-temperature vacuum drying, coating, obtains beneficial bacteria of intestinal tract preparation after detection is qualified.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of embodiment 4
Difference with embodiment 1 is only that protective agent used in step (3) includes each substance of following mass parts: 1 part of glycerol,
3 parts of oligoisomaltoses, 2 parts of oligofructose, 2.5 parts of soybean peptides, 2 parts of calcium silicates, 1 portion of brevibacterium flavum fermentation liquid inactivated,
The preparation method of the brevibacterium flavum fermentation liquid of the inactivation is that culture medium includes: glucose 40g/L, yeast powder
2g/L, ammonium sulfate 8g/L, Dried Corn Steep Liquor Powder 15g/L, potassium dihydrogen phosphate 3g/L, magnesium sulfate 0.8g/L, pH 7.2, inoculum concentration with
Brevibacterium flavum bacterium powder is calculated as 0.1g/L, and 30 DEG C are cultivated 8 hours, high-temperature sterilization.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of embodiment 5
Difference with embodiment 2 is only that protective agent used in step (3) includes each substance of following mass parts: 2 parts of glycerol,
2 parts of oligoisomaltoses, 4.5 parts of oligofructose, 0.1 part of soybean peptide, 8 parts of calcium silicates, 2 parts of brevibacterium flavum fermentations inactivated
Liquid,
The preparation method of the brevibacterium flavum fermentation liquid of the inactivation is that culture medium includes: glucose 20g/L, yeast powder
8g/L, ammonium sulfate 2g/L, Dried Corn Steep Liquor Powder 25g/L, potassium dihydrogen phosphate 0.5g/L, magnesium sulfate 1.5g/L, pH 6.9, inoculum concentration
It is calculated as 0.1g/L with brevibacterium flavum bacterium powder, 45 DEG C are cultivated 3 hours, high-temperature sterilization.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of embodiment 6
Difference with embodiment 3 is only that protective agent used in step (3) includes each substance of following mass parts: 1.5 parts sweet
Oil, 2.1 parts of oligoisomaltoses, 3.0 parts of oligofructose, 1.2 parts of soybean peptides, 6 parts of calcium silicates, 1.5 parts of yellow quarter butts inactivated
Fermented liquid；
The preparation method of the brevibacterium flavum fermentation liquid of the inactivation is that culture medium includes: glucose 30g/L, yeast powder
6g/L, ammonium sulfate 4g/L, Dried Corn Steep Liquor Powder 20g/L, potassium dihydrogen phosphate 1.5g/L, magnesium sulfate 1.1g/L；PH is 7.0；Inoculum concentration
0.1g/L is calculated as with brevibacterium flavum bacterium powder；40 DEG C are cultivated 5 hours；High-temperature sterilization.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of comparative example 1
Difference with embodiment 3 is only that the porous-starch added in step (2), which is put into step (3), neutralizes protective agent one
Addition is played, wherein the amount of the porous-starch added and protectant amount are equal with the corresponding additive amount in embodiment 3 respectively.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of comparative example 2
Difference with embodiment 6 is only that the porous-starch added in step (2), which is put into step (3), neutralizes protective agent one
Addition is played, wherein the amount of the porous-starch added and protectant amount are equal with the corresponding additive amount in embodiment 6 respectively.
A kind of industrial process of the beneficial bacteria of intestinal tract preparation of comparative example 3
Chinese patent announces preparation method shown in embodiment 3 in CN108783462 A.
Viable count statistics: beneficial bacteria of intestinal tract preparation 0.1g made from Example 1-6 and comparative example 1-3 is dissolved in appropriate nothing
(dilution 10 in bacterium water7), count plate is carried out after dilution, as a result as shown in table 1 below.
Resting period statistics: by beneficial bacteria of intestinal tract preparation made from embodiment 1-6 and comparative example 1-3, temperature 37 is deposited in
It ± 2 DEG C, in the environment of humidity 75 ± 5%, in 0th month, first month and second month, unites to the viable count in preparation
Meter, and calculates the survival rate of probiotics, and the viable count at viable count/0th the end of month at survival rate=the first or two the end of month ×
100%, it the results are shown in Table 1.
Table 1
| Experiment numbers | Viable count/(cfu/g) | One the end of month survival rate/% | Two the end of month survival rate/% |
| Embodiment 1 | 9×109 | 83 | 75 |
| Embodiment 2 | 8.2×109 | 82 | 73 |
| Embodiment 3 | 9×109 | 84 | 80 |
| Embodiment 4 | 9.5×109 | 87 | 85 |
| Embodiment 5 | 8.7×109 | 88 | 85 |
| Embodiment 6 | 9.6×109 | 91 | 86 |
| Comparative example 1 | 7.9×109 | 84 | 79 |
| Comparative example 2 | 8.0×109 | 85 | 80 |
| Comparative example 3 | 6.3×109 | 79 | 69 |
Experimental data in table 1 is shown, prepares beneficial bacteria of intestinal tract preparation using method of the invention, is able to ascend every gram of production
The quantity of viable bacteria in product, reduces protectant addition, improves the economic benefit of production.By embodiment 1-3 successively with embodiment 4-6
It is compared, it is found that on the basis of inventive method, the brevibacterium flavum fermentation liquid of inactivation, energy are added in protective agent
Enough number of viable further promoted in every gram of beneficial bacteria of intestinal tract preparation, and the addition energy of the brevibacterium flavum fermentation liquid inactivated
Inactivation rate of probiotics during preservation is enough reduced, the holding time is extended.
Claims (7)
1. a kind of industrial process of beneficial bacteria of intestinal tract preparation, which comprises the following steps:
(1) Lactobacillus casei, Lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus delbruockii subspecies bulgaricus are inoculated with respectively
Fermented and cultured is carried out into different fermentation mediums, when viable count is up to standard in fermented and cultured, is terminated fermented and cultured, is sent out
Ferment culture solution；
(2) streptococcus thermophilus is inoculated into fermentation medium and carries out fermented and cultured, added before fermentation ends into fermentation liquid more
Hole starch, obtains fermentation culture；
(3) fermentation liquid of collection step (1) and the resulting each strain of step (2) and mixing are centrifuged and obtain after washing active wet
Wet bacterium mud is added distilled water to be uniformly mixed by bacterium mud with protective agent, obtains bacterium mud protection agent composition, wherein wet bacterium mud: protective agent:
The mass ratio of distilled water is 1:0.5-0.8:0.4-0.8；
(4) by step (3) obtained bacterium mud protection agent composition successively pass through granulator, spheronizator, drying machine, seed-coating machine into
Row granulation, round as a ball, low-temperature vacuum drying, coating obtain beneficial bacteria of intestinal tract preparation after detection is qualified；
Step (2) when in fermentation liquid viable count reach 2-3 × 109When cfu/mL, porous-starch is added, it is small to continue fermented and cultured 1-5
When；
Protective agent in step (3) includes each substance of following mass parts: 1-2 parts of glycerol, 2-3 parts of oligoisomaltoses, 2-4.5
Part oligofructose, 0.1-2.5 parts of soybean peptides, 2-8 parts of calcium silicates, the 1-2 portions of brevibacterium flavum fermentation liquids inactivated.
2. industrial process according to claim 1, which is characterized in that fermentative medium formula in the step (1)
Are as follows: glucose 5.0-15.0g, pancreatin hydrolysis casein 2.0-15.0g, yeast extract 1.5-5.5g, sodium citrate 1.0-5.0g, phosphorus
Sour hydrogen dipotassium 0.2-2.5g, magnesium sulfate 0.1-0.75g, L-cysteine hydrochloride 0.001-0.1g, Tomato juice 2.0-25g,
Tween-20 0.5-2.0mL, Jia Shui are settled to 1000mL, and pH 6.0-7.0,105 DEG C sterilize 15 minutes.
3. industrial process according to claim 1, it is characterised in that: the step (1) is when viable count in fermentation liquid
Reach 3-4.5 × 109When cfu/mL, terminate fermented and cultured.
4. industrial process according to claim 1, which is characterized in that fermentation medium in the step (2) are as follows: Portugal
Grape sugar 95-98g/L, ammonium sulfate 7-10g/L, peptone 95-99g/L, ammonium nitrate 4-8g/L, potassium dihydrogen phosphate 3-4g/L, sulfuric acid
Magnesium 1-2g/L, pH 6.8-7.1,105 DEG C sterilize 15 minutes.
5. industrial process according to claim 1, it is characterised in that: the step (2) adds porous-starch, makes it
It is 10-20g/L in initial content.
6. industrial process according to claim 1, it is characterised in that: the cultivation temperature in step (1) and step (2)
It is 30 DEG C -45 DEG C.
7. industrial process according to claim 1, which is characterized in that the brevibacterium flavum fermentation liquid of the inactivation
Preparation method is that culture medium includes: glucose 20-40g/L, yeast powder 2-8g/L, ammonium sulfate 2-8g/L, Dried Corn Steep Liquor Powder 15-
25g/L, potassium dihydrogen phosphate 0.5-3g/L, magnesium sulfate 0.8-1.5g/L；PH is 6.9-7.2；Inoculum concentration is with brevibacterium flavum bacterium powder
It is calculated as 0.1g/L；30 DEG C -45 DEG C of cultures 3-8 hours；High-temperature sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910114816.XA CN109593690B (en) | 2019-02-14 | 2019-02-14 | A kind of industrial process of beneficial bacteria of intestinal tract preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910114816.XA CN109593690B (en) | 2019-02-14 | 2019-02-14 | A kind of industrial process of beneficial bacteria of intestinal tract preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109593690A CN109593690A (en) | 2019-04-09 |
| CN109593690B true CN109593690B (en) | 2019-11-12 |
Family
ID=65967328
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910114816.XA Active CN109593690B (en) | 2019-02-14 | 2019-02-14 | A kind of industrial process of beneficial bacteria of intestinal tract preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109593690B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111534566B (en) * | 2020-06-17 | 2022-02-15 | 江南大学 | Gradient diluent and application thereof in bifidobacterium counting |
| CN112553116A (en) * | 2020-12-23 | 2021-03-26 | 邢箫 | Probiotic culture medium and probiotic preparation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333840A (en) * | 2013-07-08 | 2013-10-02 | 昆山佰生优生物科技有限公司 | Probiotic ultralow temperature refrigeration technology and applications thereof in probiotic preparation |
| CN108323761A (en) * | 2017-12-27 | 2018-07-27 | 深圳市麦金利实业有限公司 | A kind of composite reactive probiotics preparation and preparation method thereof |
| CN108783462A (en) * | 2018-07-02 | 2018-11-13 | 哈尔滨美华生物技术股份有限公司 | A kind of industrial process of beneficial bacteria of intestinal tract preparation |
| CN108902315A (en) * | 2018-07-12 | 2018-11-30 | 广州华农大食品科技有限公司 | A kind of probiotic mixed fermentation Yoghourt and production method |
-
2019
- 2019-02-14 CN CN201910114816.XA patent/CN109593690B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333840A (en) * | 2013-07-08 | 2013-10-02 | 昆山佰生优生物科技有限公司 | Probiotic ultralow temperature refrigeration technology and applications thereof in probiotic preparation |
| CN108323761A (en) * | 2017-12-27 | 2018-07-27 | 深圳市麦金利实业有限公司 | A kind of composite reactive probiotics preparation and preparation method thereof |
| CN108783462A (en) * | 2018-07-02 | 2018-11-13 | 哈尔滨美华生物技术股份有限公司 | A kind of industrial process of beneficial bacteria of intestinal tract preparation |
| CN108902315A (en) * | 2018-07-12 | 2018-11-30 | 广州华农大食品科技有限公司 | A kind of probiotic mixed fermentation Yoghourt and production method |
Non-Patent Citations (1)
| Title |
|---|
| 《黄色短杆菌ilvBN和ilvC基因定点突变对L-缬氨酸发酵的影响》;宫卫波;《发酵科技通讯》;20171130;第46卷(第4期);228-232,237 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109593690A (en) | 2019-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108783462B (en) | Industrial production method of intestinal probiotic preparation | |
| CN100360658C (en) | Enteral microecological formulation and its preparation process | |
| JP5409623B2 (en) | Zinc-enriched biomass, its preparation method, and probiotic products, cosmetics, dietary products and nutritional supplements containing the same | |
| CN109593690B (en) | A kind of industrial process of beneficial bacteria of intestinal tract preparation | |
| CN111820419A (en) | Composition for targeted regulation and control of enteron-bacterium and short-chain fatty acid producing bacterium | |
| WO2018018783A1 (en) | Method for manufacturing probiotics finishing agent | |
| KR100429494B1 (en) | Manufacturing method of Protein- coated Lactic acid bacteria powder | |
| KR20120112452A (en) | Health-beneficial preparation and production method | |
| CN111671090A (en) | Probiotic composition and preparation thereof | |
| CN105559087A (en) | Probiotic product containing sialic acid and preparation method of probiotic product | |
| CN111345473A (en) | Probiotics composition containing yolk antibody IgY and application preparation | |
| KR101951893B1 (en) | Method for producing high concentration of probiotic active Lactobacillus paracasei SRCM102343 strain derived from traditional fermented food | |
| CN116622555A (en) | Composite probiotics for relieving constipation and application of composite probiotics in efficacy fermented milk product | |
| CN116590357A (en) | Application of lactobacillus reuteri in production of gamma-aminobutyric acid and sleep-aiding products | |
| KR100609779B1 (en) | Lactic acid bacteria degrading alcohol and acetaldehyde | |
| JP3954711B2 (en) | Production method of Vispan bacteria by liquid fermentation | |
| RU2471864C1 (en) | Probiotic preparation for viral and bacterial infections toxisporin, method for preparing it, bacillus licheniformis bacterial strain used as ingredient of probiotic preparation | |
| CN113397170B (en) | Application of marine prebiotics composition for regulating human intestinal flora | |
| CN117384790B (en) | Pediococcus pentosaceus KS5 and application thereof in preparation of sleep-aiding drugs | |
| CN117025488B (en) | Technological method for improving intestinal tract colonization rate of probiotics and probiotics freeze-dried powder | |
| CN117363524B (en) | Lactobacillus gasseri MY4 and application thereof in preparation of sleep-aiding and whitening medicines | |
| CN115537362A (en) | Composite bacterium for regulating human body micro-ecology and preparation method and application thereof | |
| KR100233616B1 (en) | A process for harvesting spores from culture broth of bacillus polyfermenticus | |
| CN117866839A (en) | Enterococcus faecalis XY7 and application thereof in preparation of anti-inflammatory and gout-improving foods and medicines | |
| CN117511767A (en) | Bifidobacterium longum and application thereof in improvement of autism spectrum disorder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Liang Jinzhong Inventor after: Xiao Wenjuan Inventor after: Lin Fengxiang Inventor before: Liang Jinzhong Inventor before: Xiao Wenjuan Inventor before: Lin Fengxiang |
|
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 150010 22km Airport Road, Daoli District, Harbin City, Heilongjiang Province Patentee after: HARBIN MEIHUA BIOTECHNOLOGY Co.,Ltd. Address before: 150016 No. 8-8, Shanghai Street, Daoli District, Harbin City, Heilongjiang Province Patentee before: HARBIN MEIHUA BIOTECHNOLOGY Co.,Ltd. |