CN109588407B - Constant temperature cell transfer device without power supply - Google Patents
Constant temperature cell transfer device without power supply Download PDFInfo
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- CN109588407B CN109588407B CN201710918301.6A CN201710918301A CN109588407B CN 109588407 B CN109588407 B CN 109588407B CN 201710918301 A CN201710918301 A CN 201710918301A CN 109588407 B CN109588407 B CN 109588407B
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- transfer device
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- cell transfer
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0273—Transport containers
Abstract
The present invention relates to a power-supply-free constant-temperature cell transfer device which has an effect of maintaining an optimum culture environment necessary for cell proliferation of cells contained in a first container and maintaining the activity and viability of the cells by containing the cells in the first container by accommodating the first container containing viable animal cells and a culture solution in a second container provided with a heat radiating section which allows oxygen in the atmosphere to flow in and releases heat by a redox reaction of a metal so that the optimum culture temperature is continuously supplied to a culture container even when power supply is not available.
Description
Technical Field
The present invention relates to a constant-temperature cell transfer device without a power supply, and more particularly, to a constant-temperature cell transfer device without a power supply, which supplies an optimal culture temperature even when power supply is not available, and transfers cells while maintaining a horizontal state of a container for storing the cells even when external impact is applied.
Background
Generally, cell culture includes: monolayer culture (adherent culture) in which cells are attached to a culture vessel and proliferated, and suspension culture in which cells are proliferated in a suspension state.
The cells cultured by the culture method as described above are sensitive to the culture environment. The culture environment of such cells has a great correlation with cell proliferation and survival. Therefore, in order to safely move cells sensitive to the culture environment, it is necessary to maintain a culture environment in which the cells can proliferate and survive.
In the case of general cell culture, it is necessary to continuously supply carbon dioxide and maintain a constant temperature and humidity. The most common method for transferring cells in the prior art is a method in which a cell container is placed in liquid nitrogen to form a low-temperature state and then moved.
As shown in korean laid-open patent No. 10-2015-0007636, a conventional technology for performing the above-described functions is a cell transfer machine including: the cell transfer machine comprises a conveyor case having a storage space, a cooling unit for constantly maintaining the internal temperature of the conveyor case, a heat radiating unit for radiating heat generated in the cooling process of the cooling unit to the outside of the conveyor case, a conveyor control unit for controlling the operations of the cooling unit and the heat radiating unit, and a charging power supply unit, wherein the cell transfer machine comprises: a transfer temperature controller for setting the temperature in the transfer process, displaying the temperature and recording the temperature history, an alarm device for enabling the transfer person to recognize when abnormal conditions occur in the cell transfer process, a GPS receiver for receiving GPS signals and confirming the position of the transport case, and a wireless network module for connecting the internet and the wireless network.
However, the conventional technical configuration has the following problems.
There is a problem that the activity and viability of cells are reduced when the cells are transferred after being cooled.
In addition, there is a problem that a power supply must be supplied to a device for maintaining the preservation temperature of cells.
Furthermore, there is a problem that a container for storing cells cannot be kept horizontal.
Documents of the prior art
Patent document
Patent document 1: korean laid-open patent No. 10-2015-0007636 (2015, 01, 21).
Disclosure of Invention
Problems to be solved
The present invention has been made to solve the above-described conventional problems, and an object of the present invention is to continuously supply an optimal culture temperature to a culture container without supplying power.
Further, another object of the present invention is to maintain an optimum culture temperature even if power supply is not available.
Further, it is still another object of the present invention to enable the transfer while maintaining the horizontal state of the container for storing the cells even if external impact is applied.
Means for solving the problems
In order to solve the above problems, the present invention is technically characterized by the following basic features: the first container containing the living animal cells and the culture solution is stored in the second container having a heat radiating section for allowing oxygen in the atmosphere to flow therein and releasing heat by oxidation-reduction reaction of metal, whereby the first container can be transferred while maintaining the physiological activity temperature of the cells contained in the first container.
In order to solve the above problems, the present invention is technically characterized by the following basic features: a second container having a heat generating part for allowing oxygen in the atmosphere to flow in and releasing heat by a redox reaction of a metal is provided with a third container which is accommodated in a horizontal state of a first container containing living animal cells and a culture solution, the second container can be transferred while maintaining a physiologically active temperature of the cells contained in the first container, and the third container minimizes the inclination of the first container.
In order to solve the above problems, the present invention is technically characterized by the following basic features: a second container having a heat radiating section for allowing oxygen in the atmosphere to flow in and releasing heat by a metal redox reaction is provided with a third container which is stored in a horizontal state in a first container containing viable animal cells and a culture solution, the second container can be transferred while maintaining the physiological activity temperature of the cells contained in the first container, and the third container is filled with carbon dioxide at a concentration higher than the concentration of carbon dioxide in the atmosphere while minimizing the inclination of the first container.
The present invention is characterized in that the first container is provided with a space in which a sealed state can be selectively determined, and the first container is capable of injecting a culture medium, inoculating cells, separating cells, and harvesting the cells in the space in the sealed state, and capable of culturing the cells in the open state of the space.
The invention is characterized in that the first container comprises: a closed channel for moving liquid, gas and cells from the outside to the inside of the space; a circulation filter capable of circulating a gas required for cell culture in the space; and a scraper provided in the space and configured to move to scrape the cell and detach the cell from the bottom surface of the space.
The invention is characterized in that the concentration of carbon dioxide in the first container is between 1% and 30%.
The invention is characterized in that the concentration of carbon dioxide in the third container is between 1% and 30%.
The present invention is characterized in that the temperature inside the first container is maintained at 22 to 43 ℃ by the heat radiating portion.
The present invention is characterized in that the heat radiating portion radiates heat to 40 to 60 ℃ by an oxidation-reduction reaction of oxygen and metal in the atmosphere flowing into the second container.
The present invention is characterized in that the heat radiating portion is formed by mixing a heat insulating material with a metal powder that radiates heat by reaction with oxygen.
The second container further includes an opening/closing portion for determining inflow of oxygen in the atmosphere.
The present invention is characterized in that the third container includes: an inner container having a spherical shape, having a weight provided at an inner bottom thereof, and having a first container attached to an upper portion of the weight; an outer container having a spherical shape and accommodating the inner container; and a plurality of wheels provided in such a manner as to be rolled and rotated between the inner container and the outer container.
The present invention is characterized by further comprising a fourth container for hermetically housing the first container.
The present invention is characterized in that the heat insulating material is formed by selectively mixing one or more of sawdust, salt, and water.
The invention is characterized in that the opening and closing part comprises an inflow hole which penetrates one side of the second container and enables oxygen in the atmosphere to flow into the second container; a stopper for selectively opening and closing the inflow hole; and an opening/closing member provided to the stopper and configured to cut off an inflow of oxygen when an internal temperature of the second container rises.
The present invention is characterized in that the outer container and the inner container are formed in a spherical shape by assembling a first auxiliary container and a second auxiliary container having a hemispherical shape, and a packing is provided between the first auxiliary container and the second auxiliary container.
The invention is characterized in that the weight is formed by a liquid.
The present invention is characterized in that the weight is formed of a solid having its own weight and a liquid filled in an upper portion of the solid.
The invention is characterized in that the opening and closing component is made of bimetal or temperature sensitive linear material.
The present invention is characterized in that the opening/closing member is a cylinder or a piston.
Effects of the invention
As described above, the power-supply-free constant-temperature cell transfer device according to the embodiment of the present invention has an effect of maintaining the activity and viability of cells by allowing oxygen in the atmosphere to flow into the second container having the heat radiating portion for releasing heat by the redox reaction of metal, storing the first container containing the living animal cells and the culture solution therein, and maintaining the optimal culture environment required for the cell growth of the cells contained in the first container, so as to continuously supply the optimal culture temperature to the culture container even when the power supply is not available.
In addition, the unpowered thermostatic cell transfer device of the present invention further includes an opening/closing part for determining the inflow of oxygen in the atmosphere in the second container so that the optimal culture temperature can be maintained even if power is not supplied, and has an effect of automatically cutting off the inflow of oxygen to lower the temperature if the temperature of the container rises and allowing the inflow of oxygen if the temperature of the container falls to a set temperature, thereby maintaining the optimal temperature for cell culture.
In addition, the power-supply-free constant-temperature cell transfer device according to the present invention has an effect of maintaining a horizontal state of a first container containing viable animal cells and a culture solution even if a bag is shaken or inclined during transfer by providing a third container in a second container so as to transfer the cells while maintaining the horizontal state of the container in which the cells are stored even if external impact is applied, the third container including: the container comprises an inner container provided with a weight, an outer container accommodating the inner container, and a plurality of wheels provided in such a manner as to roll between the inner container and the outer container.
Drawings
FIG. 1 is a sectional view showing a power-supply-free constant-temperature cell transfer device according to the present invention.
Fig. 2 is an exploded perspective view illustrating a third container of the unpowered thermostatic cell transfer device of the present invention.
Fig. 3 is a perspective view showing a first container of the unpowered isothermal cell transfer device of the present invention.
FIG. 4 is a sectional view showing the first container of the unpowered isothermal cell transfer device of the present invention.
FIG. 5 is a sectional view showing a state of use of the unpowered isothermal cell transfer device of the present invention.
Description of the symbols
Description of the symbols in the attached drawings
10: constant temperature cell transfer device without power supply
11: cell 12: culture solution
100: first container 101: space(s)
110: the closed passage 120: circulating filter
121: the pipe 122: valve gate
123: the filter 124 is: clamp with a locking member
130: the scraper 131: blade
132: culture tank 134: metal body
200: the second container 210: heat radiating part
220: opening/closing unit 221: inflow hole
223: stop 225: opening and closing member
300: third container 310: inner container
320: the wheel 330: outer container
340: weights 310a, 330 a: first auxiliary container
310b, 330 b: second auxiliary container 310c, 320 c: liner pad
400: the fourth container 500: bag (bag)
Detailed Description
The present invention will be described in detail below with reference to the attached drawings. It should be noted at the outset that in the drawings, identical constituent elements or components are denoted by the same reference numerals as much as possible. In describing the present invention, detailed descriptions of related well-known functions and configurations are omitted so as not to obscure the gist of the present invention.
Fig. 1 is a sectional view showing a power-free constant-temperature cell transfer device of the present invention, fig. 2 is an exploded perspective view showing a third container of the power-free constant-temperature cell transfer device of the present invention, fig. 3 is a perspective view showing a first container of the power-free constant-temperature cell transfer device of the present invention, fig. 4 is a sectional view showing the first container of the power-free constant-temperature cell transfer device of the present invention, and fig. 5 is a sectional view showing a use state of the power-free constant-temperature cell transfer device of the present invention.
First, as shown in FIG. 1, the power-less constant-temperature cell transfer device 10 according to the present invention is constructed as follows: in the second container 200 having the heat radiating portion 210 for releasing heat by oxidation-reduction reaction of metal by allowing oxygen in the atmosphere to flow in, the first container 100 containing the living animal cells 11 and the culture solution 12 is stored, and the first container 100 can be transferred while maintaining the physiological activity temperature of the cells 11 contained in the first container 100.
In addition, according to another aspect of the present invention, there is provided a power-supply-free constant-temperature-maintained cell transfer device 10, comprising a second container 200 into which oxygen in the atmosphere is introduced and which includes a heat radiating portion 210 for releasing heat by a redox reaction of a metal, wherein a third container 300 is provided, which is accommodated so as to maintain a horizontal state of a first container 100 in which living animal cells 11 and a culture solution 12 are contained, and wherein the second container 200 can transfer the cells 11 contained in the first container 100 while maintaining a physiologically active temperature thereof, and the third container 300 minimizes the inclination of the first container 100.
Further, according to still another aspect of the present invention, there is provided a power-supply-free constant temperature-maintained cell transfer device 10, comprising a second container 200 into which oxygen in the atmosphere flows and which is provided with a heat radiating portion 210 that radiates heat by a redox reaction of a metal, wherein a third container 300 is provided that is stored so as to maintain a horizontal state of the first container 100 in which the living animal cells 11 and the culture solution 12 are contained, wherein the second container 200 can transfer the cells 11 contained in the first container 100 while maintaining a physiologically active temperature thereof, and wherein the third container 300 fills the third container 300 with carbon dioxide having a concentration higher than a concentration of carbon dioxide in the atmosphere while minimizing inclination of the first container 100.
The unpowered isothermal cell transfer device 10 according to the present invention is described in more detail below.
Referring to fig. 1, a non-powered isothermal cell transfer device 10 according to a preferred embodiment of the present invention includes a first container 100 and a second container 200.
At this time, as shown in FIGS. 3 and 4, the first container 100 contains the living animal cells 11 and the culture solution 12.
A space 101 sealed inside is formed in the culture container 100.
The culture container 100 is made of a soft plastic material, and the size of the space 101 can be changed by external pressure or force.
The culture container 100 includes a space in which a sealed state can be selectively determined, and the culture container 100 is provided so that the culture solution can be injected into the space 101, cells can be inoculated, detached, and harvested in the state where the space 101 is sealed, and the cells can be cultured in the state where the space is opened.
In the process of harvesting the cells 11, cells 11 other than a part of the whole cultured cells 11 may be harvested, and the cells 11 remaining in the culture container 100 may be repeatedly cultured.
Furthermore, in the harvesting process of the cells 11, the injection of the culture solution into the culture vessel 100, the inoculation of the cells, the culture, the detachment and the harvesting may be repeatedly performed after all the cultured cells are harvested.
The culture vessel 100 is provided therein with a closed passage 110 and a circulation filter 120, respectively, so as to enable continuous culture of the cells 11 as described above.
First, the closed path 110 is provided in the culture container 100 so as to enable the processes of injecting the culture solution 12 into the space 101, seeding the cells 11 in the culture solution 12, and harvesting the cells 11, and the closed container is kept in a closed state even when the processes are performed as described above.
That is, the closed passage 110 is provided on the side of the culture vessel 100 so that the liquid, gas, and cells 11 can be moved into and out of the space 101 from the outside.
In order to realize the above process, the closed channel 110 is provided on the surface of the culture vessel 100, and the closed channel 110 is formed of a soft block and provided in the space 101.
In this case, a syringe is generally used for injecting the culture solution 12 and the cells 11 through the closed channel 110 and for harvesting the cells 11 to the outside.
In other words, the culture medium 12 filled in the syringe is injected into the space 101 after the needle of the syringe is penetrated so as to penetrate the closed passage 110, or the cells 11 detached after the culture in the culture container 100 are sucked by the syringe negative pressure, whereby the cells can be harvested from the outside.
After the culture solution 12 is injected or the cells 11 are harvested, if the needle is pulled out from the closed channel 110, the closed channel 110 itself is closed by the elasticity of the closed channel 110, and the tightness of the space 101 can be maintained.
The circulation filter 120 is provided to inject gas necessary for culturing the cells 11 into the space 101 of the culture container 100.
That is, the culture container 100 is stored in the culture environment unit 200 to be supplied at an appropriate temperature when the cells 11 are cultured, and is supplied with gas necessary for culturing the cells 11.
At this time, the culture container 100 allows gas to flow into and out of the space 101 through the circulation filter 120.
That is, the gas in the space 101 of the culture container 100 is introduced into and discharged from the space 101 necessary for culturing the cells 11 in the culture environment unit 200 by generating a negative pressure inside the culture environment unit 200 to change the size of the space 101 of the culture container 100 and passing through the circulation filter 120.
Wherein the gas includes any one of carbon dioxide and oxygen.
The circulation filter 120 is specifically configured as follows.
The circulation filter 120 includes: a pipe 121 provided on the side of the culture container 100, a valve 122 provided at the end of the pipe 121, and a filter 123 provided inside the valve 122.
A clip 124 is attached to the duct 121, and the duct 121 can be selectively closed and opened.
The clamp 124 allows gas to enter and exit the space 101 by opening the tube 121 when the culture container 100 is stored in the culture environment unit 200, and when the culture container 100 is to be removed from the culture environment unit 200, the tube 121 is clamped by the clamp 124 to seal the space 101 of the closed container.
Further, since the cells 11 cultured on the bottom surface of the space 101 of the culture container 100 are not easily detached due to the adhesion force, the scraper 130, which is another tool for detaching the cells 11, is provided inside the space 101.
That is, the scraper 130 scrapes the cell 11 while rotating or moving inside the space 101 by a mechanical external force, a magnetic force, a potential energy, or the like, thereby detaching the cell 11 from the bottom surface of the space 101.
At this time, the cell 11 is detached by the magnetic force as follows.
The moving member 140 is brought close to the lower surface of the outside of the culture vessel 100 and is connected to the scraper 130 by a magnetic force, and the scraper 130 is moved by the moving member 140 to move inside the space 101, whereby the scraper 130 scrapes and separates the cells 11 while being rubbed.
That is, the metal body 134 or the magnetic body 141 may be provided in the scraper 130 and the moving member 140, respectively, so as to be integrally interlocked with each other by means of magnetic force.
In the method of detaching cells 11 by using potential energy, culture container 100 is arranged obliquely, and scraper 130 having its own weight scrapes off cells 11 while sliding down from the bottom surface of space 101.
The scraper 130 thus configured has a culture vessel 132 formed in the upper portion thereof to fill the culture medium 12 and the cultured cells 11.
In particular, although the scraper 130 is configured as a rectangular culture container, the shape of the culture container is not limited as long as it has a surface to which cells can adhere, and may be a polygon such as a circle, triangle, or the like.
A plurality of blades 131 are formed on the lower surface of the scraper 130 contacting the bottom surface of the space 101.
Here, a plurality of blades 131 are formed on the lower surface of the scraper 130 contacting the bottom surface of the space 101, and the blades 131 are formed to have an angular shape, so that the cell 11 is detached from the bottom surface by friction with the bottom surface of the space 101.
Alternatively, a plurality of blades 131 are formed on the lower surface of the doctor blade 130 contacting the bottom surface of the space 101, and the blades 131 are formed to be continuously formed at corners so as to contact or not contact the bottom surface of the space 101.
The scraper is made of a material selected from the following materials: polyethylene (PE), polypropylene (PP), Polyamide (PA), Polyacetal (POM), polyvinyl chloride (PVC), Polyester (PET), polymethylpentene (PMP), Ionomer (IO), ethylene vinyl alcohol (EVOH), polyvinyl chloride (PVA), Polystyrene (PS), methacrylic resin (PMMA), Polycarbonate (PC), polyvinyl acetate (PVAc), polyvinyl alcohol (PVA), phenol resin (PF), urea resin (UF), melamine resin (MF), epoxy resin (EP), Polyurethane (PUR), unsaturated polyester resin (UP), and metal.
On the other hand, the first container 100 is housed in the second container 200. The second container 200 includes a heat generating portion 210 for allowing oxygen in the atmosphere to flow therein and for releasing heat by a metal redox reaction. That is, the first container 100 can be transferred while maintaining the physiological activation temperature of the cells 11 contained in the first container 100.
Also, the heat emitting part 210 emits heat to 40 to 60 ℃ by the redox reaction of oxygen and metal in the atmosphere flowing into the inside of the second container 200. In this case, the heat radiating portion 210 is formed by mixing a heat insulating material with metal powder that radiates heat by reaction with oxygen. Here, the heat insulating material is formed by selectively mixing one or more of the group consisting of sawdust, salt, and water.
That is, the temperature inside the first container 100 is maintained at 22 to 43 ℃ by the heat radiating portion 210.
As a result, the unpowered isothermal cell transfer device 10 according to the embodiment of the present invention stores the first container 100 containing the viable animal cells 11 and the culture solution 12 in the second container 200 having the heat radiating portion 210 for releasing heat by oxidation-reduction reaction of metal while allowing oxygen in the atmosphere to flow therein so that the optimal culture temperature is continuously supplied to the culture container even when power supply is not available, thereby maintaining the optimal culture environment required for cell proliferation of the cells 11 contained in the first container 100 and maintaining the activity and viability of the cells.
The second container 200 further includes an opening/closing unit 220 for determining the inflow of oxygen in the atmosphere. Such an opening/closing unit 220 includes: an inflow hole 221 penetrating one side of the second container 200 to allow oxygen in the atmosphere to flow into the second container 200, a stopper 223 to selectively open and close the inflow hole 221, and an opening and closing member 225 provided to the stopper 223 to cut off the inflow of oxygen when the internal temperature of the second container 200 rises.
Further, the opening/closing member 225 is formed of a bimetal or a temperature sensitive linear material. Further, the opening and closing member 225 is characterized by using a cylinder or a piston.
As a result, the unpowered thermostatic cell transfer device 10 of the present invention further includes an open/close unit 220 for determining the inflow of oxygen into the atmosphere in the second container 200 so that the optimal culture temperature can be maintained even if power is not supplied, and automatically cuts off the inflow of oxygen to lower the temperature if the temperature of the container rises, and allows the inflow of oxygen to maintain the optimal temperature for cell culture if the temperature of the container falls to the set temperature.
On the other hand, the second container 200 is provided with a third container 300 which is stored to maintain the horizontal state of the first container 100 containing the living animal cells 11 and the culture solution 12.
As shown in fig. 2, such a third container 300 comprises: an inner container 310 having a spherical shape, having a weight 340 at an inner bottom thereof, and having the first container 100 mounted on an upper portion of the weight 340; an outer container 330 having a spherical shape and accommodating the inner container 310; and a plurality of wheels 320 provided in such a manner as to be rolled between the inner container 310 and the outer container 330.
Further, the outer container 330 and the inner container 310 are formed in a spherical shape by assembling the first auxiliary container 300a and the second auxiliary container 300b having a hemispherical shape, and a gasket 300c is provided between the first auxiliary container 300a and the second auxiliary container 300 b.
Also, the weight 340 is formed of liquid or solid. Specifically, the weight 340 is formed of a solid having its own weight and a liquid filled in an upper portion of the solid.
As a result, as shown in fig. 5, the device 10 for transporting a passive thermostatic cell according to the present invention is configured such that a third container 300 is provided in the second container 200 so as to transport the cell while maintaining the horizontal state of the container for storing the cell even when external impact is applied, the third container 300 including: an inner container 310 provided with a weight 340, an outer container 330 accommodating the inner container 310, and a plurality of wheels 320 rotatably provided between the inner container 310 and the outer container 330 in a rolling manner, thereby maintaining a horizontal state of the first container 100 containing the living animal cells and the culture solution even if the bag is shaken or inclined during the transfer.
On the other hand, the container further includes a fourth container 400 for hermetically housing the first container 100. Such a fourth container 400 is preferably a closed container.
In particular, the concentration of carbon dioxide in the first container 100 and the third container 300 is 1% to 30%.
The terms "about", "substantially" and the like, to the extent used in this specification, are used in the sense of their numerical values or values close to their numerical values when the meaning referred to provides inherent manufacturing and material tolerances, and are intended to prevent an ill-intentioned person from improperly utilizing the disclosure of the exact or absolute numerical values referred to as being useful for understanding.
The present invention described above is not limited to the above-described embodiments and the attached drawings, and it will be apparent to those skilled in the art to which the present invention pertains that various substitutions, modifications, and changes may be made without departing from the scope of the technical idea of the present invention.
Claims (16)
1. A constant temperature cell transfer device without power supply,
a third container which is accommodated in a horizontal state of the first container containing the living animal cells and the culture solution and which is provided in the second container having a heat radiating part for releasing heat by oxidation-reduction reaction of metal by allowing oxygen in the atmosphere to flow in,
the second container can be transferred while maintaining the physiologically active temperature of the cells contained in the first container,
the tilting of the first container is minimized by the third container,
the second container further includes an opening/closing portion for determining inflow of oxygen in the atmosphere,
the opening/closing section includes:
an inflow hole penetrating one side of the second container and allowing oxygen in the atmosphere to flow into the second container;
a stopper for selectively opening and closing the inflow hole; and
an opening and closing member provided to the stopper and cutting off the inflow of oxygen when the internal temperature of the second container rises,
the third container comprises:
an inner container having a spherical shape, having a weight provided at an inner bottom thereof, and having a first container attached to an upper portion of the weight;
an outer container having a spherical shape and accommodating the inner container; and
a plurality of wheels arranged in a rolling rotation manner between the inner container and the outer container.
2. A constant temperature cell transfer device without power supply,
a third container which is accommodated in a horizontal state of the first container containing the living animal cells and the culture solution and which is provided in the second container having a heat radiating part for releasing heat by oxidation-reduction reaction of metal by allowing oxygen in the atmosphere to flow in,
the second container can be transferred while maintaining the physiological active temperature of the cells contained in the first container, the third container can be filled with carbon dioxide at a concentration higher than that in the atmosphere while minimizing the inclination of the first container,
the second container further includes an opening/closing portion for determining inflow of oxygen in the atmosphere,
the opening/closing section includes:
an inflow hole penetrating one side of the second container and allowing oxygen in the atmosphere to flow into the second container;
a stopper that selectively opens and closes the inflow hole; and
an opening and closing member provided to the stopper and cutting off inflow of oxygen when an internal temperature of the second container rises,
the third container comprises:
an inner container having a spherical shape, having a weight provided at an inner bottom thereof, and having a first container attached to an upper portion of the weight;
an outer container having a spherical shape and accommodating the inner container; and
a plurality of wheels arranged in a rolling rotation manner between the inner container and the outer container.
3. The unpowered constant temperature-retentive cell transfer device of any one of claims 1 to 2,
the first container is provided with a space capable of selectively determining a sealed state, and is capable of achieving injection of a culture solution, inoculation of cells, detachment, and harvesting into the space in a state where the space is sealed, and is capable of achieving culture of cells in a state where the space is opened.
4. The unpowered, thermostatically-maintained cell transfer device as claimed in any one of claims 1-2 wherein the first container comprises:
a closed channel for moving liquid, gas and cells from the outside to the inside of the space;
a circulation filter capable of circulating gas required for cell culture in the space; and
a scraper disposed in the space and moving to scrape the cells and detach the cells from the bottom surface of the space.
5. The unpowered constant-temperature-maintaining cell transfer device according to any one of claims 1 to 2,
the concentration of carbon dioxide in the first container is 1% to 30%.
6. The unpowered constant-temperature-maintaining cell transfer device according to any one of claims 1 to 2,
the concentration of carbon dioxide in the third container is 1% to 30%.
7. The unpowered constant-temperature-maintaining cell transfer device according to any one of claims 1 to 2,
the temperature of the inside of the first container is maintained at 22 to 43 ℃ by the heat release part.
8. The unpowered constant-temperature-maintaining cell transfer device according to any one of claims 1 to 2,
the heat releasing portion releases heat to 40 ℃ to 60 ℃ by an oxidation-reduction reaction of oxygen and metal in the atmosphere flowing into the inside of the second container.
9. The unpowered constant-temperature-maintaining cell transfer device according to any one of claims 1 to 2,
the heat-radiating portion is formed by mixing a heat-insulating material with a metal powder that reacts with oxygen to generate heat.
10. The device for transferring a cell to be transferred without a power source and held at a constant temperature according to claim 1 or 2, further comprising a fourth container for hermetically housing the first container.
11. The unpowered constant temperature-maintained cell transfer device according to claim 9,
the heat insulating material is formed by selectively mixing more than one of the group consisting of sawdust, salt and water.
12. The unpowered constant temperature-retentive cell transfer device of claim 1 or 2,
the outer container and the inner container are formed in a spherical shape by assembling a first auxiliary container and a second auxiliary container in a hemispherical shape, and a gasket is provided between the first auxiliary container and the second auxiliary container.
13. The unpowered constant-temperature-maintaining cell transfer device according to claim 1 or 2, wherein the weight is formed of a liquid.
14. The unpowered constant-temperature-maintaining cell transfer device according to claim 1 or 2,
the weight is formed of a solid having its own weight and a liquid filled in an upper portion of the solid.
15. The unpowered constant-temperature-maintaining cell transfer device according to claim 1 or 2,
the opening and closing member is made of bimetal or temperature sensitive linear material.
16. The unpowered constant temperature-retentive cell transfer device of claim 1 or 2,
the opening and closing member is a cylinder or a piston.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710918301.6A CN109588407B (en) | 2017-09-30 | 2017-09-30 | Constant temperature cell transfer device without power supply |
Applications Claiming Priority (1)
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| CN104386357A (en) * | 2014-11-16 | 2015-03-04 | 刘超农 | Buoyancy-based device capable of preventing objects from inversing and shaking and application method |
| CN105658783A (en) * | 2013-10-16 | 2016-06-08 | 麦迪康公司 | Apparatus and method for continuous cell culture |
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| CN105658783A (en) * | 2013-10-16 | 2016-06-08 | 麦迪康公司 | Apparatus and method for continuous cell culture |
| CN104386357A (en) * | 2014-11-16 | 2015-03-04 | 刘超农 | Buoyancy-based device capable of preventing objects from inversing and shaking and application method |
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