CN109580849B - Method for measuring index components in traditional Chinese medicine oral liquid - Google Patents
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Abstract
The invention discloses a method for measuring index components in traditional Chinese medicine oral liquid, which comprises the following steps: the sample solution is led into the adjustable splitter after being separated by the liquid chromatography, and the effluent liquid is divided into two paths: one path is connected with an ion mobility spectrometer, electrospray ionization is carried out under the action of spray voltage, and formed ions enter a migration tube of the ion mobility spectrometer for separation and finally reach a Faraday cup detector for detection to obtain a corresponding test map; and the other path of effluent enters a triple quadrupole mass spectrometer, and a signal of the object to be detected is collected in a multi-reaction monitoring mode, so that the detection result of the ion mobility spectrometer is further confirmed. The method for determining the index components in the traditional Chinese medicine oral liquid adopts a liquid chromatography-electrospray ionization-ion mobility spectrometry combined technology, and provides a scientific and effective technical means for quality control and active component detection of traditional Chinese medicine preparations.
Description
Technical Field
The invention relates to a detection method of chemical substances, in particular to a method for measuring 7 index components in a traditional Chinese medicine oral liquid by adopting a liquid chromatography-electrospray ionization-ion mobility spectrometry combined method.
Background
The Chinese medicine is an important component of the traditional medicine in China and an important tool for preventing and treating diseases. According to incomplete statistics, more than 130 countries and regions in the world sell traditional Chinese medicines currently, and nearly one fourth of the world population uses traditional Chinese medicines. The traditional Chinese medicine preparation is prepared into a certain dosage form for clinical direct use according to a prescription by taking the traditional Chinese medicine theory as guidance so as to achieve the aim of exerting the curative effect of the medicine to the maximum extent. The active ingredients of the traditional Chinese medicine are the key points for ensuring the clinical curative effect, and the quality control is particularly important. The establishment of the analysis and detection method of the index components of the Chinese medicinal preparation can provide guarantee for the quality control of the Chinese medicinal preparation and is also the key of modernization and internationalization of the Chinese medicinal preparation.
Ion mobility spectrometry is an analysis technology for separating and characterizing chemical substances by using the difference of migration rates of gas-phase ions formed by ionization under atmospheric pressure in an electric field, and is widely applied to the related fields of drug detection, public safety, food safety, environmental monitoring and the like. The basic principle of ion mobility spectrometry is that a sample to be detected is ionized in an ionization reaction region, generated ions enter a drift region through an ion gate which is periodically opened under the drive of electric field force, and collide with reverse neutral drift gas molecules continuously, so that the ions with different mobility rates are separated and sequentially reach a detection electrode. In ion mobility spectrometry, gas phase ions are first generated before separation and detection of product ions are performed. Existing ionization methods of ion mobility spectrometry include radioactive ionization, corona discharge ionization, photoionization, flame ionization, electrospray ionization, and the like. The electrospray ionization source is adopted, so that the ion mobility spectrometry can directly analyze the liquid sample. Electrospray ionization has become an important method for the determination of biomolecules and the analysis of environmental samples using ion mobility spectrometry.
Although ion mobility spectrometry has the advantages of simplicity, portability, low cost, rapidness, sensitivity and the like, the separation capability is limited, and under the condition that a sample matrix is relatively complex, the problems that the requirement of simultaneous separation of multiple components cannot be met, ionization competition inhibition is generated among different substances in the ionization process and the like may exist.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for measuring index components in traditional Chinese medicine oral liquid, which is simple and convenient to operate.
The ion mobility spectrometry and the liquid chromatogram are combined through an electrospray interface, the ion mobility spectrometry is used as a detector of the liquid chromatogram, and an ion mobility spectrogram obtained by measuring the effluent of a chromatographic separation column is measured, so that two-dimensional separation of the chromatogram is realized based on the difference of hydrophobicity of the chromatogram before ionization and the difference of ion mobility after ionization, richer chemical information is provided for accurate identification of complex compounds, and the analysis value of the ion mobility spectrometry is enhanced. Meanwhile, ion mobility spectrometry is a high vacuum condition required for analyzing gaseous ions under normal pressure rather than mass spectrometry, and the convenience of the combination of liquid chromatography and ion mobility spectrometry lies in the simplicity of an interface device between the two.
A method for measuring index components in traditional Chinese medicine oral liquid comprises the following steps:
the sample solution is led into the adjustable splitter after being separated by the liquid chromatography, and the effluent liquid is divided into two paths: one path is connected with an ion mobility spectrometer, electrospray ionization is carried out under the action of spray voltage, and formed ions enter a migration tube of the ion mobility spectrometer, are separated and finally reach a Faraday cup detector to be detected to obtain a corresponding test pattern; and the other path of effluent enters a triple quadrupole mass spectrometer, and a signal of the object to be detected is collected in a multi-reaction monitoring mode, so that the detection result of the ion mobility spectrometer is further confirmed.
The method for determining the index components in the traditional Chinese medicine oral liquid adopts a liquid chromatography-electrospray-ion mobility spectrometry combined experimental device, wherein the device comprises an ion mobility spectrometer, an adjustable splitter, an ultra-high performance liquid chromatograph and a triple quadrupole mass spectrometer, wherein the liquid inlet end of the adjustable splitter is connected with the liquid outlet of the ultra-high performance liquid chromatograph, and the liquid outlet end of the adjustable splitter is respectively connected with the liquid inlet ends of the ion mobility spectrometer and the triple quadrupole mass spectrometer.
The invention relates to a method for measuring index components in traditional Chinese medicine oral liquid, wherein the index components comprise 7 of danshensu, chlorogenic acid, glycyrrhizic acid, rutin, baicalin, gastrodin and puerarin.
The method for determining the index components in the traditional Chinese medicine oral liquid comprises the steps that the flow dividing ratio of the adjustable flow divider is set to be 50:1, the low-flow-rate liquid outlet end of the adjustable flow divider is connected with the liquid inlet end of the ion mobility spectrometer, and the high-flow-rate liquid outlet end of the adjustable flow divider is connected with the liquid inlet end of the triple quadrupole mass spectrometer.
The method for measuring the index components in the traditional Chinese medicine oral liquid is characterized in that the sample injection flow rate of the ion mobility spectrometer is about 1 mu L/min, and the accurate value is 0.98 mu L/min.
The invention relates to a method for measuring index components in traditional Chinese medicine oral liquid, wherein the liquid chromatographic separation conditions are as follows:
a chromatographic column: ACQUITY UPLC BEH C1850mm × 1mm,1.7 μm; flow rate: 50 mu L/min; the mobile phase A is 0.5 percent formic acid water solution, and the mobile phase B is acetonitrile; gradient elution procedure: 0 → 3min, 5% B → 10% B; 3 → 10min, 10% B → 80% B; 10 → 12min, 80% B; 12 → 12.1min, 80% B → 5% B; 12.1 → 15min, 5% B; column temperature: 25 ℃; temperature of the sample chamber: 20 ℃; sample introduction amount: 5 mu L of the solution;
the invention relates to a method for determining index components in traditional Chinese medicine oral liquid, wherein the analysis conditions of an ion mobility spectrometer are as follows:
electrospray voltage: 2400V; ionization mode: a negative ion mode; migration tube voltage: 8000V; migration tube temperature: 190 ℃; gas preheating temperature: 190 ℃; wide mobility spectrum: 26 ms; Bradbury-Nielsen ion gate pulse width: 110 mu s; Bradbury-Nielsen ion gate voltage: 37V; drifting airflow rate: 1.4L/min; pumping speed of an exhaust pump: 1.0L/min.
The invention relates to a method for measuring index components in traditional Chinese medicine oral liquid, wherein the mass spectrometry analysis conditions are as follows:
an electrospray ion source negative ion mode; capillary voltage: 2.8 kV; radio frequency lens voltage: 0.3 kV; ion source temperature: 150 ℃; desolventizing gas temperature: 500 ℃; desolventizing agent gas flow: 800L/h; taper hole gas flow: 50L/h; photomultiplier voltage: 650V; collision gas: argon gas; collision air pressure: 0.32 Pa.
The invention relates to a method for determining index components in traditional Chinese medicine oral liquid, wherein mass spectrometry parameters of 7 objects to be detected are as follows:
TABLE 17 Mass Spectrometry parameters of the analytes
Ions with higher response.
The method for measuring the index components in the traditional Chinese medicine oral liquid is different from the prior art in that: the method for determining the index components in the traditional Chinese medicine oral liquid adopts a liquid chromatography-electrospray ionization-ion mobility spectrometry combined technology, establishes an analysis method for 7 representative index components such as tanshinol, glycyrrhizic acid, gastrodin, chlorogenic acid, puerarin, baicalin, rutin and the like in the traditional Chinese medicine oral liquid, optimizes analysis parameters such as liquid chromatography, spray voltage, a migration tube, gas preheating temperature, drift gas flow rate and the like, establishes a liquid chromatography-tandem mass spectrometry confirmation method for the index components, and provides a scientific and effective technical means for quality control and active component detection of the traditional Chinese medicine preparation. The detection limit of the index components of the 7 traditional Chinese medicines is 2-10 mug/mL, and the quantification limit is 5-25 mug/mL.
The method for determining the index components in the Chinese medicinal oral liquid is further described with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of a liquid chromatography-electrospray-ion mobility spectrometry combined experimental apparatus and a flow chart in the present invention;
FIG. 2 is an ion mobility spectrum of 7 analytes in the present invention;
FIG. 3 is a selective ion chromatogram for monitoring 7 analytes in the present invention;
fig. 4 shows the signal responses of 7 analytes (n is 3) under different spray voltages in the present invention;
fig. 5 shows the signal responses (n is 3) of 7 types of analytes under different temperatures of the transfer tube and the gas preheating;
fig. 6 shows the signal responses of 7 analytes (n is 3) at different drift gas flow rates in the present invention;
FIG. 7 is a two-dimensional separation analysis of 7 analytes in the present invention.
In fig. 2, 3 and 7, the corresponding relationship between numbers and substances is:
confirming the peak of spectrum 1. danshensu; 2. glycyrrhizic acid; 3. gastrodine; 4. chlorogenic acid 5. puerarin; 6. baicalin; 7. rutin is used.
English Chinese reference table appearing in attached figure
Detailed Description
1. Experimental part
1.1 instruments and reagents
GA2100 portable ion mobility spectrometer (excellis corporation, usa): is provided with an electrospray ion source, an ion grid gate controller, an air filtering device (containing calcium sulfate and a molecular sieve), a high-resolution ion migration analyzer, a Faraday cup detector and a Vision instrument control and data processing system, wherein the instrument is respectively corrected by tryptophan and citric acid in a positive ion mode and a negative ion mode before use; QuickSplit 600-PO10-04 Adjustable shunt (Analytical Scientific Instruments, USA); ACQUITY ultra high performance liquid chromatograph, Xevo TQ-MS triple quadrupole mass spectrometer, MassLynx data processing system (Waters Corp., USA); model Milli-Q Integral 5 ultra pure water device (Merck Millipore, USA). Salvianic acid A (CAS 22681-72-7, purity 98%) and chlorogenic acid (CAS 327-97-9, purity 98%) were obtained from Beijing Zhongke quality inspection Biotechnology, Inc.; glycyrrhizic acid (CAS 1405-86-3, purity 98%), rutin (CAS 153-18-4, purity 98%), baicalin (CAS 21967-41-9, purity 98%) and gastrodin (CAS 62499-27-8, purity 98%) were purchased from Bailingwei science and technology Co., Ltd; puerarin (CAS 3681-99-0, purity 98%) was purchased from China food and drug testing research institute. Preparing 1g/L standard stock solution from 7 standard substances by using methanol, and diluting the stock solution into mixed standard working solution by using methanol according to the requirement when in use; methanol (chromatographically pure) was purchased from Fisher corporation, usa; tryptophan and citric acid were purchased from Sigma-Aldrich, USA, and were used to prepare 10mg/L of calibration solution in methanol for instrument calibration.
1.2 Experimental methods
The schematic diagram of the liquid chromatography-electrospray-ion mobility spectrometry combined experimental device built by a laboratory is shown in figure 1. After the sample solution is separated by liquid chromatography, an adjustable splitter (the split ratio is set to be 50:1) is introduced, and effluent liquid is divided into two paths: one path is connected with an ion mobility spectrometer (about 1 muL/min, the accurate numerical value is 0.98 muL/min), electrospray ionization is carried out under the action of spray voltage, and formed ions enter a migration tube of the ion mobility spectrometer for separation and finally reach a Faraday cup detector for detection to obtain a corresponding test pattern; and the other path of effluent enters a triple quadrupole mass spectrometer, and signals of 7 objects to be detected are collected in a multi-reaction monitoring mode, so that the ion mobility spectrometry detection result can be further confirmed.
1.3 conditions of liquid chromatography separation
A chromatographic column: ACQUITY UPLC BEH C18(50 mm. times.1 mm,1.7 μm); flow rate: 50 mu L/min; the mobile phase A is 0.5% formic acid water solution, and the mobile phase B is acetonitrile. Gradient elution procedure: 0 → 3min, 5% B → 10% B; 3 → 10min, 10% B → 80% B; 10 → 12min, 80% B; 12 → 12.1min, 80% B → 5% B; 12.1 → 15min, 5% B; column temperature: 25 ℃; temperature of the sample chamber: 20 ℃; sample introduction amount: 5 μ L.
1.4 ion mobility spectrometry conditions
Electrospray voltage: 2400V; ionization mode: a negative ion mode; migration tube voltage: 8000V; migration tube temperature: 190 ℃; gas preheating temperature: 190 ℃; wide mobility spectrum: 26 ms; Bradbury-Nielsen ion gate pulse width: 110 mu s; Bradbury-Nielsen ion gate voltage: 37V; drifting airflow rate: 1.4L/min; pumping speed of an exhaust pump: 1.0L/min. The molecular formulae, relative molecular masses, and migration times of the 7 analytes are shown in Table 2, and the ion mobility spectrum is shown in FIG. 2.
TABLE 27 molecular formulas, relative molecular masses, ionization patterns, and migration times of the analytes
1.5 conditions for Mass Spectrometry
An electrospray ion source negative ion mode; capillary voltage: 2.8 kV; radio frequency lens voltage: 0.3 kV; ion source temperature: 150 ℃; desolventizing gas temperature: 500 ℃; desolventizing agent gas flow: 800L/h; taper hole gas flow: 50L/h; photomultiplier voltage: 650V; collision gas: argon gas; collision air pressure: 0.32 Pa. The mass spectrometry parameters of the 7 analytes are shown in Table 1, and the multiple reaction monitoring chromatogram is shown in FIG. 3.
2 results and analysis
2.1 optimization of the conditions for liquid chromatography separation
When the ion mobility spectrometry is used with the liquid chromatography, an electrospray ionization source is the most common interface mode, and generates charged liquid drops and ions of an object to be detected through a high-voltage electric field, and then the charged liquid drops and the ions enter the ion mobility spectrometry for detection. In the experiment, an electrospray interface equipped for the ion mobility spectrometry is not provided with a built-in auxiliary gas and a heating module, so that the tolerable flow rate range is low. The research selects the micro-diameter column ACQUITY UPLC BEH C by comprehensively considering the factors of applicable flow rate, separation efficiency, chromatographic peak shape, compatibility with an electrospray interface and the like18(50 mm. times.1 mm,1.7 μm). By comparison, acetonitrile was used as the mobile phaseThe organic solvent can obtain better chromatographic performance and signal response. Since the 7 analytes are all acidic compounds, a certain proportion of volatile acid needs to be added to the mobile phase water phase to obtain the desired chromatographic peak shape and retention behavior.
2.2 optimization of ion mobility Spectroscopy spray Voltage
The spray voltage is an important parameter for determining the signal response intensity and ionization effect of the target component. After the sample is separated by liquid chromatography, the post-column effluent is shunted by a shunt, and ions are generated to enter an ion mobility spectrum under the action of spray voltage to obtain a response signal. In the experiment, response values of 7 traditional Chinese medicine index components are investigated under the condition that the spraying voltage is 1600-2600V, the optimal spraying voltages of the 7 traditional Chinese medicine index components are slightly different, the optimal spraying voltage values and the ionization effects of the 7 compounds to be detected are comprehensively considered, and the spraying voltage of the method is determined to be 2400V. The results of the experiment are shown in FIG. 4.
2.3 optimization of ion mobility spectrometry mobility tubes and gas preheat temperatures
The research investigates the influence of the ion mobility spectrometry migration tube and the gas preheating temperature on the signal intensity of the target compound. In order to avoid the problems of poor reproducibility of ion migration time, unstable response value and the like caused by heat exchange or other factors, experiments set the two parameters to be the same value when the two parameters are optimized. When the temperature is too low, water molecules in the environment can interfere with ion signals; excessive temperatures can cause system instability, resulting in ion loss. The influence of different temperatures (160, 170, 180, 190, 200 ℃) on the response values of the index components of the 7 traditional Chinese medicines is respectively examined, and the result is shown in figure 5. Comprehensively considering the signal intensity of each target compound ion, the stability of signal drift time and the peak shape performance, and selecting 190 ℃ as the preheating temperature of the migration tube and the gas.
2.4 optimization of ion mobility Spectroscopy drift gas flow Rate
The species and flow rate of the drift gas have some effect on ion resolution and response strength. Air is selected as drift gas by virtue of the advantages of strong stability, low cost and the like. The research investigates the influence of different drift air flow rates (1.2-2.2L/min) on the separation effect and the response intensity of 7 compounds, and the experimental result is shown in figure 6. When the drift gas flow rate is too low, the 7 compounds have poor resolution; when the flow rate is too high, the intensity of the response signal is reduced, which may be caused by dilution of the target concentration, cancellation of the effect of the electric field force, and difficulty in reaching the detector by the ions. The optimum flow rate of the drift gas was investigated to be 1.4L/min.
2.5 liquid chromatography-electrospray-ion mobility spectrometry analysis
The liquid chromatogram and the ion mobility spectrometry are respectively based on different properties of the object to be measured and are separated according to different mechanisms, and the two-dimensional separation systems formed by the separation systems can complement each other in separation capacity. On the basis of respectively optimizing conditions of a liquid chromatogram and an ion mobility spectrometry, the two-dimensional separation analysis method of 7 index components such as tanshinol, glycyrrhizic acid, gastrodin, chlorogenic acid, puerarin, baicalin, rutin and the like is developed by combining the liquid chromatogram with the ion mobility spectrometry, and an obtained two-dimensional contour map is shown in figure 7. The detection limit, quantitative limit, linear range, linear equation and related coefficient of the index components of the 7 traditional Chinese medicines are shown in table 3.
Since the working flow rates of conventional liquid chromatography columns are typically on the order of mL/min, the flow rate range that electrospray ion mobility spectrometry can tolerate is low, typically on the order of μ L/min, and there is no match between the two. Too high a sample introduction flow rate can make desolvation of electrospray difficult, thereby increasing noise of ion mobility spectrometry and reducing response signals of ions. The invention adopts a micro-diameter chromatographic column with the proper flow rate of about 50 mu L/min and the inner diameter of 1mm, and obtains the low flow rate through post-column shunting so as to adapt to the compatible requirement of electrospray ion mobility spectrometry. However, the conventional liquid chromatograph has a relatively large external column volume, so that the separation dimension of the liquid chromatograph has a certain peak shape expansion. In subsequent experimental studies, a nanoliter/nanoliter liquid chromatograph is used for chromatographic separation to achieve better separation effect.
TABLE 37 Linear equation, Linear Range, correlation coefficient, detection limit and quantitation limit for the test substances
Y is peak area; x is mass concentration, mu g/mL.
2.6 confirmation of liquid chromatography-triple quadrupole mass spectrometry
The research also develops a liquid chromatography-triple quadrupole mass spectrometry confirmation method for 7 index components such as tanshinol, glycyrrhizic acid, gastrodin, chlorogenic acid, puerarin, baicalin, rutin and the like. For each target compound, one precursor ion and two corresponding product ions are respectively selected as a monitoring ion pair, and the mass spectrometry parameters of 7 components to be detected are shown in table 2. If the relative abundance of the ions of the target compound in the sample is consistent with that of the standard solution with the equivalent concentration, and the deviation does not exceed the corresponding allowable deviation in table 2, the sample is judged to contain the corresponding target compound.
2.7 sample determination
The method is used for detecting and analyzing 5 actual samples of the traditional Chinese medicine oral liquid such as the children's fever abatement oral liquid, the qingkailing oral liquid, the children's lung heat clearing and phlegm reducing oral liquid, the children's seven-star tea oral liquid, the Shuanghuanglian oral liquid and the like. Through determination, the content of index components glycyrrhizic acid in the traditional Chinese medicine oral liquid sample is 730.69 and 841.82 mug/mL, and the content of baicalin is 4.22, 6.51 and 13.08mg/mL respectively, which all meet the content requirement in the pharmacopoeia of the people's republic of China 2015 edition.
3 conclusion
The research adopts a liquid chromatography-electrospray ionization-ion mobility spectrometry combined technology, establishes a separation and analysis method for 7 index components such as danshensu, glycyrrhizic acid, gastrodin, chlorogenic acid, puerarin, baicalin, rutin and the like in the traditional Chinese medicine oral liquid, combines the characteristics of high resolution, quick separation and orthogonality with liquid chromatography separation of the ion mobility spectrometry, can realize two-dimensional separation of a target compound based on the difference of hydrophobicity and ion mobility, and provides more abundant comprehensive information for comprehensive analysis of a complex sample system.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (5)
1. A method for measuring index components in traditional Chinese medicine oral liquid is characterized in that: the method comprises the following steps:
the sample solution is led into the adjustable splitter after being separated by the liquid chromatography, and the effluent liquid is divided into two paths: one path is connected with an ion mobility spectrometer, electrospray ionization is carried out under the action of spray voltage, and formed ions enter a migration tube of the ion mobility spectrometer, are separated and finally reach a Faraday cup detector to be detected to obtain a corresponding test pattern; the other path of effluent enters a triple quadrupole mass spectrometer, and a signal of an object to be detected is collected in a multi-reaction monitoring mode, so that the detection result of the ion mobility spectrometer is further confirmed;
the index components are 7 of danshensu, chlorogenic acid, glycyrrhizic acid, rutin, baicalin, gastrodin and puerarin;
the liquid chromatographic separation conditions were:
a chromatographic column: ACQUITY UPLC BEH C1850mm × 1mm,1.7 μm; flow rate: 50 mu L/min; the mobile phase A is 0.5 percent formic acid water solution, and the mobile phase B is acetonitrile; gradient elution procedure: 0 → 3min, 5% B → 10% B; 3 → 10min, 10% B → 80% B; 10 → 12min, 80% B; 12 → 12.1min, 80% B → 5% B; 12.1 → 15min, 5% B; column temperature: 25 ℃; temperature of the sample chamber: 20 ℃; sample introduction amount: 5 mu L of the solution;
the analysis conditions of the ion mobility spectrometer are as follows:
electrospray voltage: 2400V; ionization mode: a negative ion mode; migration tube voltage: 8000V; migration tube temperature: 190 ℃; gas preheating temperature: 190 ℃; wide mobility spectrum: 26 ms; Bradbury-Nielsen ion gate pulse width: 110 mu s; Bradbury-Nielsen ion gate voltage: 37V; drifting airflow rate: 1.4L/min; pumping speed of an exhaust pump: 1.0L/min;
the mass spectrometry conditions were as follows:
an electrospray ion source negative ion mode; capillary voltage: 2.8 kV; radio frequency lens voltage: 0.3 kV; ion source temperature: 150 ℃; desolventizing gas temperature: 500 ℃; desolventizing agent gas flow: 800L/h; taper hole gas flow: 50L/h; photomultiplier voltage: 650V; collision gas: argon gas; collision air pressure: 0.32 Pa.
2. The method for determining the index components in the Chinese medicinal oral liquid according to claim 1, wherein the method comprises the following steps: the experimental device comprises an ion mobility spectrometer, an adjustable splitter, an ultra-high performance liquid chromatograph and a triple quadrupole mass spectrometer, wherein a liquid inlet end of the adjustable splitter is connected with a liquid outlet of the ultra-high performance liquid chromatograph, and a liquid outlet end of the adjustable splitter is respectively connected with liquid inlet ends of the ion mobility spectrometer and the triple quadrupole mass spectrometer.
3. The method for determining the index components in the Chinese medicinal oral liquid according to claim 2, wherein the method comprises the following steps: the flow dividing ratio of the adjustable flow divider is set to be 50:1, the low-flow-rate liquid outlet end of the adjustable flow divider is connected with the liquid inlet end of the ion mobility spectrometer, and the high-flow-rate liquid outlet end of the adjustable flow divider is connected with the liquid inlet end of the triple quadrupole rod mass spectrometer.
4. The method for determining the index components in the Chinese medicinal oral liquid according to claim 3, wherein the method comprises the following steps: the sample injection flow rate of the ion mobility spectrometer is 0.98 mu L/min.
5. The method for determining the index components in the Chinese medicinal oral liquid according to claim 4, wherein the method comprises the following steps:
the mass spectrometry parameters of the 7 analytes are as follows:
ions with higher response.
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