CN109576245A - Barley diet barley enzyme preparation and its zymotechnique - Google Patents

Barley diet barley enzyme preparation and its zymotechnique Download PDF

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Publication number
CN109576245A
CN109576245A CN201811608744.6A CN201811608744A CN109576245A CN 109576245 A CN109576245 A CN 109576245A CN 201811608744 A CN201811608744 A CN 201811608744A CN 109576245 A CN109576245 A CN 109576245A
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barley
enzyme preparation
fermentation
diet
zymotechnique
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范志恒
王海彦
李玉欣
周坤
刘茹
王春艳
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BEIJING HUAMEIYUAN BIOLOGICAL TECHNOLOGY CO LTD
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BEIJING HUAMEIYUAN BIOLOGICAL TECHNOLOGY CO LTD
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Abstract

The invention discloses a kind of barley diet zymotechniques of barley enzyme preparation, the zymotechnique selects strain aspergillus niger GDMCC 3.452, expand culture through PDA slant activation, wheat bran and obtains spore as fermentation seed, to add the barley skin of rice husk as fermentation substrate, it is carried out solid fermentation 6~7 days at 28~30 DEG C of temperature, obtains barley enzyme preparation.The zymotechnique of the barley diet of acquisition barley enzyme preparation, the fermentation carried out under the induction of specifically fermentation substrate by specified strain, there is specificity, the multienzyme system barley enzyme preparation of high efficiency to barley degradation to be formed, conventional barley enzyme is changed using the production form of single enzyme compounding, and significantly improves barley enzyme to the degradation of barley diet.Barley diet provided by the invention barley enzyme preparation, can not only give full play to the degradation of apoenzyme and its association enzyme, moreover it is possible to utilize the synergistic effect of apoenzyme and association enzyme, degradation barley anti-nutritional factors, nutrient digestibility is improved, enteron aisle burden is reduced, improves gastrointestinal health.

Description

Barley diet barley enzyme preparation and its zymotechnique
Technical field
The present invention relates to fodder enzyme preparation field more particularly to a kind of zymotechnique of barley diet barley enzyme preparation, And the barley enzyme preparation obtained by the zymotechnique.
Background technique
Barley is a kind of important grain trough raw material, cheap, but due to containing glucan, xylan in barley Etc. anti-nutritional factors, cause animal metabolism ability poor, the low problem of feeding value, to limit barley answering in daily ration With.Usually improve the quality of barley feed in a manner of adding barley enzyme preparation at present, in view of the particularity of barley nutritional ingredient, Conventional barley enzyme is to obtain single enzyme compounding such as zytase, dextranase, but this complex enzyme formulation imitates the degradation of barley Fruit is unsatisfactory, tends not to meet livestock rearing demand.
Summary of the invention
To solve the shortcomings of the prior art, the present invention provides a kind of fermentation works of barley diet barley enzyme preparation Skill has specificity, high efficiency to barley degradation to be formed by carrying out induction fermentation using specified strain and specifically fermentation substrate Multienzyme system barley enzyme preparation.
To achieve the above object, the zymotechnique of barley diet provided by the invention barley enzyme preparation, the fermentation work Skill selects strain aspergillus niger GDMCC 3.452, expands culture through PDA slant activation, wheat bran and obtains spore as fermentation seed, with The barley skin of rice husk is added as fermentation substrate, is carried out solid fermentation 6~7 days at 28~30 DEG C of temperature, obtains barley enzyme system Agent；
The additive amount of rice husk is 11~13% in the fermentation substrate.
The zymotechnique of barley diet of the invention barley enzyme preparation, using the specified strain of screening as zymophyte, with Barley skin is that fermentation substrate carries out induction fermentation, and is especially added with a small amount of rice husk in the solid fermentation process of barley skin, with enhancing The gas permeability of fermentation substrate obtains the barley enzyme preparation of multienzyme system to guarantee going on smoothly for specific substrates induction fermentation.This The zymotechnique of invention changes conventional barley enzyme in a manner of single enzyme compounding, and the induction by specified strain in specific substrates is sent out Ferment form obtains having specificity, the multienzyme system barley enzyme preparation of high efficiency degradation to barley diet, and more traditional compounding is big Wheat enzyme, the more conducively raising and nutrient absorption of livestock.
As the restriction to above-mentioned technical proposal, the zymotechnique the following steps are included:
A, the aspergillus niger GDMCC 3.452 of freezing: being inoculated into the inclined-plane PDA to have sterilized by actication of culture, temperature 29~ 31 DEG C, under the conditions of humidity 80~90%, cultivate 5~6 days, obtain spore；
B, expand culture: by the spore inoculating on inclined-plane to bran mass, in 29~31 DEG C of temperature, humidity 80~90% Under the conditions of, it cultivates 5~7 days, a large amount of black spores is generated, as fermentation seed；
C, solid fermentation: after taking 37~39 parts of barley skins, 11~13 parts of rice husks to be mixed, 50 parts of water is added to stir evenly Afterwards, it is configured to fermentation substrate, solid medium of the thickness up to 4.5~5.5cm, inoculation fermentation kind are obtained after fermentation substrate is sterilized Son, under the conditions of guaranteeing oxygen supply abundance, 28~30 DEG C of temperature, fermentation obtains barley enzyme preparation in 6~7 days.
The operating procedure for further providing for barley fermentation using enzyme technique enables the zymotechnique to implement perfectly, from And obtain the barley enzyme preparation of more excellent performance.
As the restriction to above-mentioned technical proposal, bran mass described in step b includes dry wheat bran 44~50%, moisture 50 ~56%.
As the restriction to above-mentioned technical proposal, the inoculum concentration of the fermentation seed is the solid culture of 30~50kg of dry weight Base is inoculated with 40~50g fermentation seed.
Further limit the factors such as bran mass, fermentation seed inoculum concentration, optimization for fermentation technology and barley enzyme preparation Energy.
Meanwhile the present invention also provides a kind of barley diet barley enzyme preparations, the barley enzyme preparation is by the barley The zymotechnique of daily ration barley enzyme preparation is made.
As the restriction to above-mentioned technical proposal, the barley enzyme preparation includes that 6000~6800U/g of dextranase, wood are poly- 10000~11000U/g of carbohydrase.
As the restriction to above-mentioned technical proposal, the barley enzyme preparation further includes pectase, cellulase, mannosan Enzyme, protease.
As the restriction to above-mentioned technical proposal, the barley enzyme preparation be added to barley diet usage amount be 500~ 1000g/t。
The other compositions of barley enzyme preparation and the usage amount of barley enzyme preparation are further limited, the barley enzyme obtained is made Preparation most effectively improves feeding value, improves the digestibility of nutriment.
Barley diet provided by the invention barley enzyme preparation is the complex enzyme formulation of multienzyme, with dextranase, xylan Enzyme is apoenzyme, and supplemented by the association enzyme for generation of fermenting, glucan and xylan in efficient degradation barley, association enzyme cooperate with apoenzyme Anti-nutritional factors in degradation barley improves gastrointestinal peristalsis to reduce the viscosity in enteron aisle, accelerates gastrointestinal emptying, and promote Contact of the nutriment with endogenous enzymes, and then improve the digestion and absorption of nutriment.
In conclusion using technical solution of the present invention, the zymotechnique of the barley diet barley enzyme preparation of acquisition leads to It crosses and carries out induction fermentation using specified strain and specifically fermentation substrate, and added during fermentation substrate carries out solid fermentation A small amount of rice husk, to solve the problems, such as that fermentation substrate is generated in sterilization process, to guarantee going on smoothly for induction fermentation；Change simultaneously Become conventional barley enzyme in a manner of single enzyme compounding, obtains the multienzyme system barley that there is specificity, high efficiency to barley degradation Enzyme preparation.Barley diet provided by the invention barley enzyme preparation, can give full play to the degradation of apoenzyme and its association enzyme, The synergistic effect that apoenzyme and association enzyme can also be utilized, barley anti-nutritional factors of degrading, improves nutrient digestibility, and it is negative to reduce enteron aisle Load improves gastrointestinal health, improves livestock rearing effect conscientiously.
Specific embodiment
Below in conjunction with embodiment, technical solution of the present invention is clearly and completely described, it is clear that described Embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field Those of ordinary skill's every other embodiment obtained without making creative work, belongs to protection of the present invention Range.
Embodiment one
The present embodiment is related to a kind of barley diet barley enzyme preparation and its zymotechnique.
Embodiment 1.1
A kind of zymotechnique of barley diet barley enzyme preparation, the zymotechnique sequentially include the following steps:
A, the aspergillus niger GDMCC 3.452 of freezing: being inoculated into the inclined-plane PDA to have sterilized by actication of culture, 30 DEG C of temperature, Under the conditions of humidity 85%, cultivates 5 days, obtain spore；
B, expand culture: by the spore inoculating on inclined-plane to the wheat bran culture containing 52% moisture and 48% dry wheat bran Base is cultivated 5 days under the conditions of 30 DEG C of temperature, humidity 85%, a large amount of black spores is generated, as fermentation seed；
C, it solid fermentation: takes 39 parts of barley skins, 11 parts of rice husks to be added in blender, and stir while adding, adds 50 parts of water stir 5min, are configured to 100 parts of quality of fermentation substrate, and the solid that thickness reaches 5cm is obtained after fermentation substrate is sterilized Culture medium, the inoculation fermentation seed on solid medium, fermentation seed inoculum concentration are calculated with the dry weight of solid medium, are done The weight for referring to barley and rice husk in solid medium again, is inoculated with 40g fermentation seed on the solid medium of dry weight 30kg, Guarantee oxygen supply it is sufficient, under the conditions of 28 DEG C of temperature, fermentation obtains barley enzyme preparation in 7 days；
It is detected and contains dextranase 6054U/g, zytase 10625U/g, pectase, fiber in the barley enzyme preparation Plain enzyme, mannonase protease；It is different according to the content of barley that the barley enzyme preparation is added to the usage amount in barley diet Depending on, barley enzyme preparation additive amount is 500g/t in full barley diet.
Embodiment 1.2
A kind of zymotechnique of barley diet barley enzyme preparation, the zymotechnique sequentially include the following steps:
A, the aspergillus niger GDMCC 3.452 of freezing: being inoculated into the inclined-plane PDA to have sterilized by actication of culture, 31 DEG C of temperature, Under the conditions of humidity 85%, cultivates 6 days, obtain spore；
B, expand culture: by the spore inoculating on inclined-plane to the bran mass containing 56% moisture and 44% dry wheat bran, Under the conditions of 31 DEG C of temperature, humidity 85%, cultivates 7 days, a large amount of black spores are generated, as fermentation seed；
C, it solid fermentation: takes 38 parts of barley skins, 12 parts of rice husks to be added in blender, and stir while adding, adds 50 parts of water stir 5min, are configured to 100 parts of quality of fermentation substrate, and the solid that thickness reaches 5cm is obtained after fermentation substrate is sterilized Culture medium, the inoculation fermentation seed on solid medium, fermentation seed inoculum concentration are calculated with the dry weight of solid medium, are done The weight for referring to barley and rice husk in solid medium again, is inoculated with 45g fermentation seed on the solid medium of dry weight 32kg, Guarantee oxygen supply it is sufficient, under the conditions of 30 DEG C of temperature, fermentation obtains barley enzyme preparation in 6 days；
Through containing dextranase 6723U/g, zytase 10813U/g, pectase, cellulose in detection barley enzyme preparation Enzyme, mannonase protease；The barley enzyme preparation be added to the usage amount in barley diet it is different according to the content of barley and Fixed, barley enzyme preparation additive amount is 500g/t in the daily ration containing barley 20%.
Embodiment 1.3
A kind of zymotechnique of barley diet barley enzyme preparation, the zymotechnique sequentially include the following steps:
A, the aspergillus niger GDMCC 3.452 of freezing: being inoculated into the inclined-plane PDA to have sterilized by actication of culture, 30 DEG C of temperature, Under the conditions of humidity 90%, cultivates 6 days, obtain spore；
B, expand culture: by the spore inoculating on inclined-plane to the bran mass containing 50% moisture and 50% dry wheat bran, Under the conditions of 30 DEG C of temperature, humidity 90%, cultivates 6 days, a large amount of black spores are generated, as fermentation seed；
C, it solid fermentation: takes 37 parts of barley skins, 13 parts of rice husks to be added in blender, and stir while adding, adds 50 parts of water stir 5min, are configured to 100 parts of quality of fermentation substrate, and the solid that thickness reaches 5cm is obtained after fermentation substrate is sterilized Culture medium, the inoculation fermentation seed on solid medium, fermentation seed inoculum concentration are calculated with the dry weight of solid medium, are done The weight for referring to barley and rice husk in solid medium again, is inoculated with 50g fermentation seed on the solid medium of dry weight 45kg, Guarantee oxygen supply it is sufficient, under the conditions of 30 DEG C of temperature, fermentation obtains barley enzyme preparation in 7 days；
Through containing dextranase 6351U/g, zytase 10906U/g, pectase, cellulose in detection barley enzyme preparation Enzyme, mannonase protease；The barley enzyme preparation be added to the usage amount in barley diet it is different according to the content of barley and Fixed, barley enzyme preparation additive amount is 800g/t in the daily ration containing barley 40%.
Embodiment two
The present embodiment is related to the service performance verifying of barley enzyme preparation that zymotechnique of the present invention obtains in barley diet.
Using the zymotechnique being the same as example 1, the barley enzyme system that mean parameter in embodiment one is fermented Agent carries out evaluating in vitro, using function and effect of the evaluating in vitro method detection barley enzyme in barley diet, as a result such as following table institute Show:
As seen from the above table, in barley: corn: dregs of beans: rapeseed meal is to add 500g/t barley enzyme in 2:5:1.5:1.5 daily ration Afterwards, dry matter disappearance rate improves 1.23%, and protease hydrolysate, which can be worth, improves 50cal/g；In barley: corn: dregs of beans: rapeseed meal 4:3: In 1.5:1.5 daily ration, after adding 800g/t barley enzyme, dry matter disappearance rate improves 1.44%, and protease hydrolysate can be worth raising 73cal/g；In full barley diet, after adding 1000g/t barley enzyme, dry matter disappearance rate improves 2.04%, and protease hydrolysate can be worth Improve 81cal/g；So as to obtain the barley enzyme preparation obtained using zymotechnique of the present invention in the day of different barley ratios The digestion of nutriment can be improved in grain, and barley content is higher in daily ration, improvement is more obvious.
Evaluating in vitro is carried out by feed substrate of full barley, it is found that barley enzyme preparation produced by the present invention has barley viscosity It significantly improves, the result measured is as shown in the table:
As seen from the above table: in full barley add barley enzyme preparation after, be significantly improved to barley viscosity, make viscosity from 22.03 drop to 6.20, reduce 71.86%, illustrate anti-nutritional factors significant effect in barley enzyme degradation barley.
Embodiment three
The present embodiment be related to strain in zymotechnique of the present invention, fermentation substrate to barley enzyme preparation the performance in barley diet Influence.
Embodiment 3.1
It is obtained using zymotechnique identical with embodiment 1.1, detection by different strain or different fermentations fermenting substrate Function and effect of the barley enzyme preparation in full barley diet, additive amount of the barley enzyme preparation in full barley diet is 1000g/ T, measurement result are as shown in the table below:
As seen from the above table: the barley enzyme preparation of strain or fermentation substrate fermentation outside using the scope of the invention, though it is degradable Barley anti-nutrition component, but effect is poorer than barley enzyme preparation of the invention；It can be seen that the fermentation work of barley enzyme preparation of the present invention In skill, the barley enzyme preparation of generation of fermenting under conditions of fermentation substrate in specified strain and range to the degradation anti-nutrition of barley at Divide significant effect.
Embodiment 3.2
Using zymotechnique identical with embodiment 1.1, the fermentation substrate without rice husk is taken to ferment to obtain barley enzyme preparation, It detects and contains dextranase 5489U/g, zytase 6325U/g in thus obtained barley enzyme preparation.
Due to using fermentation substrate not containing rice husk, fermentation seed leads to fermentation not for hypoxgia during the fermentation Completely, glucolase and xylanase activity are all relatively low in the barley enzyme preparation obtained, it can be seen that, in fermentation substrate The content of rice husk is most important in zymotechnique of the barley diet of the present invention with barley enzyme preparation.
Comparative example
Certain company's barley complex enzyme, by single enzyme such as zytase, dextranase, pectase, cellulase, mannase It is compounded with protease etc., and each single enzyme is liquid fermentation, compounded formula is as shown in the table:
Serial number Material name Indicate enzyme activity U/g Match %
1 Zytase 1 200,000 3.5
2 Zytase 2 200,000 6.0
3 Acid mannase 50,000 3.0
4 1,4 beta-glucanase 50,000 10.0
5 Cellulase 10,000 8.0
6 Amylase 5,000 10.0
7 Protease 50,000 22.5
8 Pectase 30,000 3.6
9 Powdered rice hulls 33.4
It is total 100
The said firm's barley complex enzyme is added in full barley diet and carries out evaluating in vitro, and barley complex enzyme is in Quan great Mai Additive amount in daily ration is 1000g/t, uses function and effect of the evaluating in vitro method detection barley enzyme in barley diet, detection Obtain: dry matter disappearance rate is 81.85%, and protease hydrolysate can value 3165cal/g, viscosity 9.65cP.
Thus obtain: barley enzyme preparation made from zymotechnique of the present invention is significantly mentioned than conventional barley enzyme preparation using effect Height, and have a clear superiority.
In conclusion zymotechnique of the barley diet of the present invention with barley enzyme preparation, mainly by specified strain with big Fermentation substrate supplemented by wheat Pi Weizhu, rice husk carries out induction fermentation, and this zymotechnique not only changes the life of conventional barley enzyme Production mode is no longer compounded with single enzyme, and obtains efficient multienzyme complex enzyme formulation of degrading to barley diet, while the hair The complex enzyme formulation that ferment technique obtains has specificity, high efficiency based on apoenzyme, supplemented by association enzyme, to barley diet degradation.

Claims (8)

1. a kind of barley diet zymotechnique of barley enzyme preparation, it is characterised in that: the zymotechnique selects the black song of strain Mould GDMCC 3.452 expands culture through PDA slant activation, wheat bran and obtains spore as fermentation seed, to add the barley of rice husk Skin carries out solid fermentation 6~7 days at 28~30 DEG C of temperature as fermentation substrate, obtains barley enzyme preparation；
The additive amount of rice husk is 11~13% in the fermentation substrate.
2. the barley diet according to claim 1 zymotechnique of barley enzyme preparation, which is characterized in that the fermentation work Skill the following steps are included:
A, the aspergillus niger GDMCC 3.452 of freezing: being inoculated into the inclined-plane PDA to have sterilized by actication of culture, 29~31 DEG C of temperature, Under the conditions of humidity 80~90%, cultivates 5~6 days, obtain spore；
B, expand culture: by the spore inoculating on inclined-plane to bran mass, in 29~31 DEG C of temperature, 80~90% condition of humidity Under, it cultivates 5~7 days, a large amount of black spores is generated, as fermentation seed；
C, solid fermentation: after taking 37~39 parts of barley skins, 11~13 parts of rice husks to be mixed, add 50 parts of water after mixing evenly, match Fermentation substrate is made, obtains solid medium of the thickness up to 4.5~5.5cm after fermentation substrate is sterilized, inoculation fermentation seed, Guarantee oxygen supply it is sufficient, under the conditions of 28~30 DEG C of temperature, fermentation obtains barley enzyme preparation in 6~7 days.
3. the barley diet according to claim 2 zymotechnique of barley enzyme preparation, which is characterized in that described in step b Bran mass includes dry wheat bran 44~50%, moisture 50~56%.
4. the barley diet according to claim 2 zymotechnique of barley enzyme preparation, it is characterised in that: the fermentation kind The solid medium that the inoculum concentration of son is 30~50kg of dry weight is inoculated with 40~50g fermentation seed.
5. a kind of barley diet barley enzyme preparation, it is characterised in that: the barley enzyme preparation is by any in Claims 1 to 4 The zymotechnique of the item barley diet barley enzyme preparation is made.
6. barley diet according to claim 5 barley enzyme preparation, it is characterised in that: the barley enzyme preparation includes Portugal 6000~6800U/g of dextranase, 10000~11000U/g of zytase.
7. barley diet according to claim 6 barley enzyme preparation, it is characterised in that: the barley enzyme preparation further includes Pectase, cellulase, mannonase protease.
8. barley diet according to claim 5 barley enzyme preparation, it is characterised in that: the barley enzyme preparation is added to The usage amount of barley diet is 500~1000g/t.
CN201811608744.6A 2018-12-27 2018-12-27 Barley diet barley enzyme preparation and its zymotechnique Pending CN109576245A (en)

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