CN109573322A - A kind of gelatin/chamomile essential oil nanofiber biological dressing and edible packaging and preparation method thereof - Google Patents
A kind of gelatin/chamomile essential oil nanofiber biological dressing and edible packaging and preparation method thereof Download PDFInfo
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- CN109573322A CN109573322A CN201910022822.2A CN201910022822A CN109573322A CN 109573322 A CN109573322 A CN 109573322A CN 201910022822 A CN201910022822 A CN 201910022822A CN 109573322 A CN109573322 A CN 109573322A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/463—Edible packaging materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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Abstract
The present invention provides a kind of preparation methods of nanofiber Wound dressing, comprising: A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, obtain spinning solution；B) by spinning solution electrostatic spinning, nanofiber is obtained；C nanofiber is crosslinked in cross-linking reagent), is dried to obtain nanofiber wound dressing.It is provided by the invention then that obtained nanofiber Wound dressing good biocompatibility is crosslinked by gelatin and chamomile essential oil blend spinning, cytotoxic evil is acted on.The present invention also provides a kind of preparation methods of edible package, comprising: A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, obtain spinning solution；B) by spinning solution electrostatic spinning, edible package is obtained.Gelatin and chamomile essential oil blend spinning are obtained edible package by the present invention, and raw material is natural, good biocompatibility, are acted on cytotoxic evil.
Description
Technical field
The present invention relates to medical material technology and food packaging applications, more particularly, to a kind of nanofiber Wound dressing and
Edible package and preparation method.
Background technique
How tissue prevents bacterium infection in therapeutic process and promotes by wound or the postoperative wound left
Wound healing is the clinical problem faced.Therefore, selecting suitable dressing is the pass of correct processing wound and accelerating wound healing
Key.At present over the course for the treatment of using more for materials such as petrolatum gauze, Iodophor gauze and non-woven fabrics.But in these dressing
In antibacterial, reduction wound infection, promoting wound healing aspect, the effect is unsatisfactory.Biological dressing is the wound in propositions such as Winter
Wound repairs the novel wound reparation and protection materials to grow up in " wet healing " theoretical basis.It is raw compared with traditional dressing
Object dressing has many advantages, such as to reduce infection, improves wound healing quality, mitigates the pain of patient and medical staff is facilitated to operate,
Therefore the favor of people has been obtained.
Common biological dressing is prepared by materials such as collagen, protein, chitosan, hyaluronic acids, they have hemostasis
Blood coagulation enhancing effect can promote various kinds of cell Proliferation, Differentiation.Gelatin is the product of collagen partial hydrolysis, the glue of amino acid composition
Former similar, good biocompatibility, and there is strong hydrophily, good film-forming property, biodegradable, special in typical both sexes dielectric
Good physics and chemical property, the lot of documents such as property have studied gelatin in dressing, pharmaceutical carrier, biological support, packaging material
Deng application.For biological dressing, because gelatin does not have bioactivity, the simple dressing prepared using gelatin is not
Have the bioactivity such as antibacterial, anti-oxidant.The prior art commonly supported nano-gold category ion, such as loading nano silvery
Grain, but the toxicity of nano metal ion pair human body cell still has dispute, and the concern with people to natural extract
With the research of natural products pharmacological and beneficial functions, also day is cumulative for application of the volatile oil in terms of Wound dressing and food packaging
It is long.
Food packaging is to protect food from external environmental, extends food and saves quality and time.But business is a large amount of
The polymer plastic packaging film of synthesis is used, although the barrier property of this material is good, plastics are mainly using petroleum as base
Plinth is made, and not only increases the consumption of petroleum, and since it is difficult to degrade, cause serious environmental pollution extensively.With
The development of modern food industry, food packaging constantly update, one kind can be improved contradictory between packaging material and environmental protection
Novel foodstuff packing technique material -- edible packaging is shown one's talent.Edible packing material refers to when the function of packaging is realized
Afterwards, which can be changed into a kind of animal or the edible raw material of people, be a kind of the special of achievable packaging material Function transition
Packaging material.Edible packing material is a kind of packaging of no waste, is a kind of packaging material of resource-type environment-friendly type
With edible packaging, generally by starch, protein, polysaccharide, fat, complex class material composition, it is widely used in such as preservative film, packet
Fill film, food packaging, cake packaging, seasoning packaging etc..
And edible package disclosed in the prior art is mostly usually required by chitosan and fibroin albumen etc. and is synthesized high score
Sub- material (PVA, PLA etc.) mixing is used as spinning solution, and excellent bioactivity, which is unable to get, to be fully played.And due to
The presence of high molecular material, toxicity, biocompatibility and biodegradability are all less desirable.
Summary of the invention
In view of this, the technical problem to be solved in the present invention is that providing a kind of nanofiber wound dressing and one kind can
Edible packaging, nanofiber wound dressing biocompatibility provided by the invention, stability, release performance are good.The present invention provides
Edible package good biocompatibility, toxicity is low, and antioxidant effect is good.
The present invention provides a kind of preparation methods of nanofiber wound dressing, comprising:
A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, spinning solution is obtained；
B) by spinning solution electrostatic spinning, nanofiber is obtained；
C nanofiber is crosslinked in cross-linking reagent), is dried to obtain nanofiber wound dressing.
Preferably, the cross-linking reagent includes 1,2- dichloroethanes and N- hydroxysuccinimide.
Preferably, the molar concentration of 1, the 2- dichloroethanes is 0.002-0.2M/mL, the N- hydroxysuccinimide
Molar concentration be 0.002-0.2M/mL；The crosslinking time is 2~8h.
Preferably, step A) mass concentration of the gelatin in spinning solution is 0.1~2g/mL；The chamomile essence
The additional amount of oil is 20~100 μ l/mL；The mass ratio of acetic acid and water is (20~25): (1~5) in the acetic acid solution.
Preferably, the electrospinning parameters specifically: spinneret voltage is 8~21kV；Pump discharge is 0.2~2ml/h, spray
Silk the distance between crown end and coverslip are 5~15cm.
The present invention provides a kind of nanometer of wound dressing, the preparation of the preparation method as described in above-mentioned technical proposal any one
It obtains.
The present invention provides a kind of preparation methods of edible package, comprising:
A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, spinning solution is obtained；
B) by spinning solution electrostatic spinning, edible package is obtained.
Preferably, step A) mass concentration of the gelatin in spinning solution is 0.1~2g/mL；The chamomile essence
The additional amount of oil is 20~100 μ l/mL；The mass ratio of acetic acid and water is (20~25): (1~5) in the acetic acid solution.
Preferably, the electrospinning parameters specifically: spinneret voltage is 8~21kV；Pump discharge is 0.2~2ml/h, spray
Silk the distance between crown end and coverslip are 5~15cm.
The present invention provides a kind of edible package, the preparation method as described in above-mentioned technical proposal any one is prepared into
It arrives.
Compared with prior art, the present invention provides a kind of preparation methods of nanofiber wound dressing, comprising: A) it will be bright
Glue, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, obtain spinning solution；B) by spinning solution electrostatic spinning, nanometer is obtained
Fiber；C nanofiber is crosslinked in cross-linking reagent), is dried to obtain nanofiber wound dressing.It is provided by the invention by gelatin
It is then crosslinked obtained nanofiber wound dressing good biocompatibility with chamomile essential oil blend spinning, cytotoxic evil is made
With nanoscale web has high porosity and big unit bodies specific surface area, and similar to extra-cellular matrix structure, has
Conducive to cell adherence and proliferation, impaired skin can be substituted and play temporary barrier action, have good breathable moisture permeability and
Stability provides suitable environment for tissue self-healing reparation and wound healing, and the addition of oil of chamomile improves the anti-of the dressing
Oxidation effectiveness, the peat-reek of oil of chamomile also can relieving mood, meet and enjoyed on patient mental.
The present invention also provides a kind of preparation methods of edible package, comprising: A) gelatin, acetic acid aqueous solution and ocean is sweet
Chrysanthemum essential oil stirring and dissolving, obtains spinning solution；B) by spinning solution electrostatic spinning, edible package is obtained.The present invention is by gelatin
With chamomile essential oil blend spinning, edible package is obtained, without the addition of high molecular material, good biocompatibility, to cell
Nonhazardous effect, release is good, while cytotoxicity is low, and the addition of oil of chamomile improves the anti-oxidant and antibacterial effect of the dressing
Fruit also improves the hydrophobicity of gelatine nano fiber, it is possible to reduce because of oxidative damage, bacteria breed etc. causes food to rot, can
Extend the time of food storage.
Detailed description of the invention
Fig. 1 is that the product that the embodiment of the present invention 6 obtains and single glutin nano fabric film that spins carry out SEM measurement result figure；
Fig. 2 is the product that the embodiment of the present invention 6 obtains and single spinning glutin nano fabric film diameter distribution results figure；
Fig. 3 is the nuclear-magnetism result for the chamomile essential oil that the embodiment of the present invention 3 obtains；
Fig. 4 is single nuclear-magnetism result for spinning gelatine nano fiber that the embodiment of the present invention 3 obtains；
Fig. 5 is the nuclear-magnetism result of 3 essential oil content 9% of the embodiment of the present invention；
Fig. 6 is that the product of the embodiment of the present invention 3 carries out essential oil sustained release figure；
Fig. 7 is single water contact angle for spinning glutin nano fabric film；
Fig. 8 is the water contact angle of the glutin nano fabric film containing 9% chamomile essential oil；
Fig. 9 is that the product of the embodiment of the present invention 4~6 being prepared carries out DPPH removing experimental result；
Figure 10 is plate plate colonies number of the product being prepared to Escherichia coli of the embodiment of the present invention 4~6；
Figure 11 is plate plate colonies of the product being prepared to staphylococcus aureus of the embodiment of the present invention 4~6
Number；
Figure 12 is that the product of the embodiment of the present invention 1~3 being prepared carries out MTT test result figure.
Specific embodiment
The present invention provides a kind of nanofiber wound dressing and a kind of edible package, those skilled in the art can be with
Present disclosure is used for reference, component, realization of process parameters are suitably modified.In particular, it should be pointed out that all similar substitutions and modifications
Apparent to those skilled in the art, they shall fall within the protection scope of the present invention.It method of the invention and answers
With being described by preferred embodiment, related personnel can obviously not depart from the content of present invention, in spirit and scope
To methods herein and application is modified or appropriate changes and combinations, carrys out implementation and application the technology of the present invention.
The present invention provides a kind of preparation methods of nanofiber wound dressing, comprising:
A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, spinning solution is obtained；
B) by spinning solution electrostatic spinning, nanofiber is obtained；
C nanofiber is crosslinked in cross-linking reagent), is dried to obtain nanofiber wound dressing.
The present invention by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, obtains spinning solution first.
The present invention for above-mentioned gelatin, acetic acid aqueous solution and chamomile essential oil addition sequence without limit, can mix
Conjunction.The present invention for the stirring and dissolving concrete mode without limit, it is well known to those skilled in the art；It is excellent
Choosing can be to be dissolved using magnetic stirrer.The time of the stirring is preferably 1~3h.It is molten to which spinning be prepared
Liquid.
By spinning solution electrostatic spinning, nanofiber is obtained.
The present invention for the electrostatic spinning specific steps without limit, it is well known to those skilled in the art.
It is preferred that specifically: the syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply, setting
The bias voltage of 8~21kV, cathode are connected on the roller that diameter is 8~15cm.Then clean coverslip is fixed on rolling
For collecting nanofiber on cylinder.
Wherein, mass concentration of the gelatin in spinning solution is preferably 0.1~2g/mL；More preferably 0.1~0.5g/mL；
The additional amount of the chamomile essential oil is preferably 20~100 μ l/mL；More preferably 30~90 μ l/mL；It is specifically as follows 30 μ l/
ML, 60 μ l/mL and 90 μ l/mL.
The mass ratio of acetic acid and water is preferably (20~25): (1~5) in the acetic acid solution.
The electrospinning parameters are preferred specifically: spinneret voltage is 8~21kV；More preferably 10~18kV；Pump discharge
For 0.2~2ml/h, more preferably 0.2~1.5ml/h；Most preferably 0.2~1.0ml/h；
The distance between spinning head top and coverslip are 5~15cm；More preferably 7~14cm；Most preferably 8~
13cm；It is the most preferably 10~12cm.
After electrostatic spinning, the coverslip for having collected nanofiber is placed in vacuum desiccator dry 20~for 24 hours to remove
Remaining solvent.
The invention avoids existing conventional casting film-forming, is obtained by the way of gelatin and chamomile essential oil spinning
To nanofiber, essential oil quickly volatilizees caused by avoiding because of hot conditions.
The invention selects chamomile essential oil and gelatin as spinning solution, using electrostatic spinning technique preparation one
Kind nanofiber wound dressing.
Wherein, gelatin is become white by the collagenous portion degradation in the connective tissues such as animal skin, bone, sarolemma, flesh evil spirit
Color or faint yellow, translucent, micro-strip gloss thin slice or powder have good biocompatibility and biodegradability.
Chamomile essential oil is that the highly concentrated liquid extracting is extracted from anthemidis flos, with special fragrance, is had
It can promote cytothesis；Bactericidal antiphlogistic；It pacifies calm；The functions such as strengthen immunity.The milkiness of chamomile essential oil and gelatin preparation
For liquid as spinning solution, spun nanofiber membrane structure is good, relatively it is single spin gelatin fiber film have preferably promote cytothesis and
Antioxidation.An especially prominent advantage of chamomile essential oil is the antiallergic problem of releiving to skin, is " most gentle by honor
Skin makeup strength ", even the tender skin of baby can use, therefore chamomile essential oil has very greatly in the research of wound dressing
Development potentiality.
The spinning solution is capable of forming one layer of good compact nanometer tunica fibrosa under electrostatic spinning technique.The tunica fibrosa is straight
Diameter is between 100-800nm, and hole is through having certain permeability and hydrophobicity generally between 5-50 μm.The present invention is for institute
The source of chamomile essential oil is stated without lower restriction, it is well known to those skilled in the art.
Nanofiber is crosslinked in cross-linking reagent, is dried to obtain nanofiber wound dressing.
Cross-linking reagent sufficiently dissolves in ethanol solution is made crosslinker solution.
Cross-linking reagent of the present invention preferably includes 1,2- dichloroethanes and N- hydroxysuccinimide.
Wherein, the molar concentration of 1, the 2- dichloroethanes is preferably 0.002-0.2M/mL, more preferably 0.01-
0.18M/mL；Most preferably 0.05-0.15M/mL；
The molar concentration of the N- hydroxysuccinimide is preferably 0.002-0.2M/mL；More preferably 0.01-0.18M/
mL；Most preferably 0.05-0.15M/mL.
The coverslip for having nanofiber will be collected and be placed in 2~8h of crosslinking in cross-linking reagent.After the completion of crosslinking, sample with ethanol
Rinsing three times and is dried in a vacuum 20~for 24 hours to remove remaining chemicals.The crosslinking time is preferably 3~7h；More
Preferably 4~6h.
Gelatin is crosslinked by the invention, improves its permeability, the performances such as stability, and effect is good.
The present invention provides a kind of preparation methods of nanofiber wound dressing, comprising: A) by gelatin, acetic acid aqueous solution and
Chamomile essential oil stirring and dissolving, obtains spinning solution；B) by spinning solution electrostatic spinning, nanofiber is obtained；C) by Nanowire
Dimension is crosslinked in cross-linking reagent, is dried to obtain nanofiber wound dressing.It is provided by the invention to be mixed by gelatin and chamomile essential oil
It closes spinning and is then crosslinked obtained nanofiber wound dressing good biocompatibility, cytotoxic evil is acted on, nanoscale is fine
Tieing up net has high porosity and big unit bodies specific surface area, and similar to extra-cellular matrix structure, is conducive to cell adherence
With proliferation, impaired skin can be substituted and play temporary barrier action, there is good breathable moisture permeability and stability, for tissue
Self-healing reparation and wound healing provide suitable environment, the peat-reek of oil of chamomile also can relieving mood, meet patient mental
Upper enjoyment.The gelatin of preparation/chamomile essential oil nanofiber wound dressing has certain plant fragrance taste, can be to a certain degree
The peculiar smell that wound suppuration generates is covered, also there is certain antioxidant effect, moreover it is possible to accelerate to eliminate the nervous of patient, alleviate
Pressure, pleasure mood meet the spiritual enjoyment of people.
The present invention provides a kind of nanometer of wound dressing, the preparation of the preparation method as described in above-mentioned technical proposal any one
It obtains.
The above-mentioned preparation method of the present invention has been clearly described, and details are not described herein.
The present invention provides a kind of preparation methods of edible package, comprising:
A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, spinning solution is obtained；
B) by spinning solution electrostatic spinning, edible package is obtained.
The present invention by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, obtains spinning solution first.
The present invention for above-mentioned gelatin, acetic acid aqueous solution and chamomile essential oil addition sequence without limit, can mix
Conjunction.The present invention for the stirring and dissolving concrete mode without limit, it is well known to those skilled in the art；It is excellent
Choosing can be to be dissolved using magnetic stirrer.The time of the stirring is preferably 1~3h.It is molten to which spinning be prepared
Liquid.
By spinning solution electrostatic spinning, edible package is obtained.
The present invention for the electrostatic spinning specific steps without limit, it is well known to those skilled in the art.
It is preferred that specifically: the syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply, setting
The bias voltage of 8~21kV, cathode are connected on the roller that diameter is 8~15cm.Then clean coverslip is fixed on rolling
For collecting nanofiber, as edible package on cylinder.
Wherein, mass concentration of the gelatin in spinning solution is preferably 0.1~2g/mL；More preferably 0.1~0.5g/mL；
The additional amount of the chamomile essential oil is preferably 20~100 μ l/mL；More preferably 30~90 μ l/mL；It is specifically as follows 30 μ l/
ML, 60 μ l/mL and 90 μ l/mL.
The mass ratio of acetic acid and water is preferably (20~25): (1~5) in the acetic acid solution.
The electrospinning parameters are preferred specifically: spinneret voltage is 8~21kV；More preferably 10~18kV；Pump discharge
For 0.2~2ml/h, more preferably 0.2~1.5ml/h；Most preferably 0.2~1.0ml/h；
The distance between spinning head top and coverslip are 5~15cm；More preferably 7~14cm；Most preferably 8~
13cm；It is the most preferably 10~12cm.
After electrostatic spinning, the coverslip for having collected nanofiber is placed in vacuum desiccator dry 20~for 24 hours to remove
Remaining solvent.
The invention avoids existing conventional casting film-forming, is obtained by the way of gelatin and chamomile essential oil spinning
To edible package, the quick volatilization of essential oil caused by avoiding because of hot conditions.
The invention selects chamomile essential oil and gelatin as spinning solution, using electrostatic spinning technique preparation one
Kind edible package.
Wherein, gelatin is become white by the collagenous portion degradation in the connective tissues such as animal skin, bone, sarolemma, flesh evil spirit
Color or faint yellow, translucent, micro-strip gloss thin slice or powder have good biocompatibility and biodegradability.
Chamomile essential oil is that the highly concentrated liquid extracting is extracted from anthemidis flos, with special fragrance, is had
It can promote cytothesis；Bactericidal antiphlogistic；It pacifies calm；The functions such as strengthen immunity.There is a large amount of document report chamomile essential oil
With excellent antioxidant effect.
Emulsion prepared by chamomile essential oil and gelatin is as spinning solution, and the spinning solution is under electrostatic spinning technique
It is capable of forming one layer of good compact nanometer tunica fibrosa.The tunica fibrosa diameter is between 100-800nm, and hole is through generally at 5-50 μm
Between, there is certain permeability and hydrophobicity.The present invention for the chamomile essential oil source without lower restriction, ability
Known to field technique personnel.
This patent selects gelatin, and chamomile essential oil prepares Wound dressing and edible package.It is different from traditional thin film-forming method,
This patent prepares gelatin/chamomile essential oil nano fibrous membrane using electrostatic spinning, and hot conditions is not only avoided to volatilize essential oil
It influences, moreover, nanoscale fiber porosity is high, good permeability is conducive to cell adherence and proliferation；With good barrier
Can, for example, optical barrier, liquid barrier etc..
The present invention also provides a kind of preparation methods of edible package, comprising: A) gelatin, acetic acid aqueous solution and ocean is sweet
Chrysanthemum essential oil stirring and dissolving, obtains spinning solution；B) by spinning solution electrostatic spinning, edible package is obtained.The present invention is by gelatin
With chamomile essential oil blend spinning, edible package is obtained, without the addition of high molecular material, good biocompatibility, to cell
Nonhazardous effect, and there is good ultravioletlight screening to act on and remove the ability of free radical, it can contract to a certain extent
The time of short food oxidation.Gelatin/chamomile essential oil nanofiber wound dressing of invention preparation has certain plant virtue
Fragrance can cover the peculiar smell of wound suppuration generation to a certain degree, also have certain antioxidant effect, moreover it is possible to accelerate to eliminate and suffer from
Person's is nervous, relieves stress, pleasure mood, meets the spiritual enjoyment of people.
The present invention provides a kind of edible package, the preparation method as described in above-mentioned technical proposal any one is prepared into
It arrives.
The above-mentioned preparation method of the present invention has been clearly described, and details are not described herein.
In order to further illustrate the present invention, with reference to embodiments to a kind of nanofiber wound dressing provided by the invention
And a kind of edible package is described in detail.
Embodiment 1
A kind of preparation method of nanofiber wound dressing, includes the following steps:
(1) preparation of spinning solution
It weighs 0.12g gelatin to be dissolved in the solvent of 880 μ l acetic acid and 120 μ l distilled water, 3% chamomile essence is then added
Oil, spinning solution is made to being completely dissolved in stirring 4h under magnetic stirring apparatus.
(2) electrostatic spinning of spinning solution
Syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply to obtain
The bias voltage of 15kV, cathode are connected on the roller that diameter is 10cm.Then clean coverslip is fixed on roller and is used
In collection nanofiber.The flow of pump is 10cm on 0.2ml/h, the distance between spinning head top and coverslip.Electrostatic spinning
Afterwards, the coverslip for having collected nanofiber is placed in vacuum desiccator dried overnight to remove remaining solvent.
(3) crosslinking of nanofiber
It takes 0.002302g EDC and 0.003834g NHS sufficiently to dissolve in 1ml ethanol solution and crosslinker solution is made.It will
Collecting, which has the coverslip of nanofiber to be placed in crosslinker solution, is crosslinked 4h.After the completion of crosslinking, sample with ethanol rinsing three times and
Dry a whole night is in vacuum to remove remaining chemicals.
Embodiment 2
A kind of preparation method of nanofiber wound dressing, includes the following steps:
(1) preparation of spinning solution
It weighs 0.12g gelatin to be dissolved in the solvent of 880 μ l acetic acid and 120 μ l distilled water, 6% chamomile essence is then added
Oil, spinning solution is made to being completely dissolved in stirring 4h under magnetic stirring apparatus.
(2) electrostatic spinning of spinning solution
Syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply to obtain
The bias voltage of 15kV, cathode are connected on the roller that diameter is 10cm.Then clean coverslip is fixed on roller and is used
In collection nanofiber.The flow of pump is 10cm on 0.2ml/h, the distance between spinning head top and coverslip.Electrostatic spinning
Afterwards, the coverslip for having collected nanofiber is placed in vacuum desiccator dried overnight to remove remaining solvent.
(3) crosslinking of nanofiber
It takes 0.002302g EDC and 0.003834g NHS sufficiently to dissolve in 1ml ethanol solution and crosslinker solution is made.It will
Collecting, which has the coverslip of nanofiber to be placed in crosslinker solution, is crosslinked 4h.After the completion of crosslinking, sample with ethanol rinsing three times and
Dry a whole night is in vacuum to remove remaining chemicals.
Embodiment 3
A kind of preparation method of nanofiber wound dressing, includes the following steps:
(1) preparation of spinning solution
It weighs 0.12g gelatin to be dissolved in the solvent of 880 μ l acetic acid and 120 μ l distilled water, 9% chamomile essence is then added
Oil, spinning solution is made to being completely dissolved in stirring 4h under magnetic stirring apparatus.
(2) electrostatic spinning of spinning solution
Syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply to obtain
The bias voltage of 15kV, cathode are connected on the roller that diameter is 10cm.Then clean coverslip is fixed on roller and is used
In collection nanofiber.The flow of pump is 10cm on 0.2ml/h, the distance between spinning head top and coverslip.Electrostatic spinning
Afterwards, the coverslip for having collected nanofiber is placed in vacuum desiccator dried overnight to remove remaining solvent.
(3) crosslinking of nanofiber
It takes 0.002302g EDC and 0.003834g NHS sufficiently to dissolve in 1ml ethanol solution and crosslinker solution is made.It will
Collecting, which has the coverslip of nanofiber to be placed in crosslinker solution, is crosslinked 4h.After the completion of crosslinking, sample with ethanol rinsing three times and
Dry a whole night is in vacuum to remove remaining chemicals.
Embodiment 4
A kind of preparation method of edible package, includes the following steps:
(1) preparation of spinning solution
It weighs 0.12g gelatin to be dissolved in the solvent of 880 μ l acetic acid and 120 μ l distilled water, 3% chamomile essence is then added
Oil, spinning solution is made to being completely dissolved in stirring 4h under magnetic stirring apparatus.
(2) electrostatic spinning of spinning solution
Syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply to obtain
The bias voltage of 15kV, cathode are connected on the roller that diameter is 10cm.Then clean coverslip is fixed on roller and is used
In collection nanofiber.The flow of pump is 10cm on 0.2ml/h, the distance between spinning head top and coverslip.Electrostatic spinning
Afterwards, the coverslip for having collected nanofiber is placed in vacuum desiccator dried overnight to remove remaining solvent, obtained edible
With packing.
Embodiment 5
A kind of preparation method of edible package, includes the following steps:
(1) preparation of spinning solution
It weighs 0.12g gelatin to be dissolved in the solvent of 880 μ l acetic acid and 120 μ l distilled water, 6% chamomile essence is then added
Oil, spinning solution is made to being completely dissolved in stirring 4h under magnetic stirring apparatus.
(2) electrostatic spinning of spinning solution
Syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply to obtain
The bias voltage of 15kV, cathode are connected on the roller that diameter is 10cm.Then clean coverslip is fixed on roller and is used
In collection nanofiber.The flow of pump is 10cm on 0.2ml/h, the distance between spinning head top and coverslip.Electrostatic spinning
Afterwards, the coverslip for having collected nanofiber is placed in vacuum desiccator dried overnight to remove remaining solvent, obtained edible
With packing.
Embodiment 6
A kind of preparation method of edible package, includes the following steps:
(1) preparation of spinning solution
It weighs 0.12g gelatin to be dissolved in the solvent of 880 μ l acetic acid and 120 μ l distilled water, 9% chamomile essence is then added
Oil, spinning solution is made to being completely dissolved in stirring 4h under magnetic stirring apparatus.
(2) electrostatic spinning of spinning solution
Syringe with spinning solution is mounted on syringe pump.Spinneret is connected on the anode of power supply to obtain
The bias voltage of 15kV, cathode are connected on the roller that diameter is 10cm.Then clean coverslip is fixed on roller and is used
In collection nanofiber.The flow of pump is 10cm on 0.2ml/h, the distance between spinning head top and coverslip.Electrostatic spinning
Afterwards, the coverslip for having collected nanofiber is placed in vacuum desiccator dried overnight to remove remaining solvent, obtained edible
With packing.
Embodiment 7
The product and single glutin nano fabric film that spins obtain to the embodiment of the present invention 6 carries out SEM and diameter measure of spread, knot
Fruit shows to mix the fiber morphology of nanofiber and single nanofiber pattern class for spinning gelatin after chamomile essential oil as shown in Figure 1:
Seemingly, it can be formed smoothly, the uniform and nano fibrous membrane that is evenly distributed.Distribution of fiber diameters is analyzed with Image J software, as a result
As shown in Fig. 2, wherein single aperture for spinning gelatin is in 300-400nm, and the fiber aperture of 9% Peppermint essential oil is in 400-500nm, this
It is because the addition of chamomile essential oil increases the viscosity of spinning solution, so that the diameter of nanofiber be made to increase, this also illustrates
Chamomile essential oil mixes successfully.
Embodiment 8
Implement the product that 3 obtain to the present invention to carry out with single glutin nano fabric film that spins1H-NMR measurement.Fig. 3 is chamomile
The nuclear-magnetism of essential oil as a result, Fig. 4 be single nuclear-magnetism for spinning gelatine nano fiber as a result, Fig. 5 is the nuclear-magnetism of essential oil content 9% as a result, its
The characteristic peak positions of middle chamomile essential oil are in 1.1ppm and 1.6ppm, and the characteristic peak of gelatin is in 1.2ppm and 1.9ppm, Cong Tuzhong
As can be seen that gelatin and the characteristic peak of chamomile essential oil occur in gelatin/chamomile essential oil nanofiber.
Embodiment 9
The product of the embodiment of the present invention 3 being prepared and single gelatin that spins are subjected to essential oil sustained release experiment, as a result such as figure five
It is shown, wherein Fig. 6 is that the product of the embodiment of the present invention 3 carries out essential oil sustained release figure, and as seen from Figure 6, the present invention passes through electrostatic
Slowly, effect keeps lasting to the release of spinning essential oil.
Embodiment 10
The product of the embodiment of the present invention 4~6 being prepared and single gelatine nano fiber that spins are subjected to water contact angle test,
Specially the nanofiber of collection on the cover slip is placed in vacuum desiccator overnight, nanometer is surveyed using water contact angle measuring instrument
The contact angle of fiber.As a result as shown in Figure 7 and Figure 8, Fig. 7 is single water contact angle for spinning glutin nano fabric film, and Fig. 8 is chamomile
Essential oil/glutin nano fabric film water contact angle.It can be seen from the figure that the product hydrophobic effect of the embodiment of the present invention 4~6
Good, for Wound dressing, hydrophobic effect is good easily peelable, reduces the feeling of pain of patient.It is hydrophobic for edible package
Effect can extend the food holding time well to avoid the exchange of moisture between atmosphere and food.
Embodiment 11
The product of the embodiment of the present invention 4~6 being prepared is subjected to DPPH and removes experiment, specially preparation 0.1mmol/
L DPPH solution, takes the nanofiber of about 60mg to be placed in six orifice plates, adds the configured DPPH solution of 3ml, is protected from light and is incubated for 30min
Afterwards, it takes out solution and surveys its absorbance value at 517nm with ultraviolet specrophotometer.As a result as shown in figure 9, can be seen by Fig. 9
Out, the antioxidant effect of the product of the embodiment of the present invention 4~6 enhances as the additional amount of chamomile essential oil increases.Wherein, thin
Clearance rate of the lotus content at 9% reaches 45% or so.
Embodiment 12
The product of the embodiment of the present invention 4~6 being prepared is subjected to bacteriostatic experiment, as a result as shown in Figure 10 and Figure 11,
Figure 10 is plate plate colonies number of the product being prepared to Escherichia coli of the embodiment of the present invention 4~6；Figure 11 is the present invention
Plate plate colonies number of the product of embodiment 4~6 being prepared to staphylococcus aureus.As can be seen that package ocean is sweet
The nano fibrous membrane of chrysanthemum essential oil has good fungistatic effect.
Embodiment 13
The product of the embodiment of the present invention 1~3 being prepared is subjected to MTT test, specially takes the Nanowire of about 60mg
It is placed in six orifice plates after dimension photograph is ultraviolet, the complete medium of 10% serum of 3ml is added, be added to culture afterwards every other day to logarithmic phase
3T3 cell 96 orifice plates in, pass through MTT experiment detect nanofiber cytotoxicity.It as a result as shown in figure 12, can by Figure 12
To find out, the Nanowire of the low as can be seen from the figure various concentration Peppermint essential oil of the product cytotoxicity of the embodiment of the present invention 1~3
Under dimension, the survival rate of cell is 85% or more, it can be deduced that, the nanofiber good biocompatibility, no cytotoxicity.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of nanofiber wound dressing, comprising:
A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, spinning solution is obtained；
B) by spinning solution electrostatic spinning, nanofiber is obtained；
C nanofiber is crosslinked in cross-linking reagent), is dried to obtain nanofiber wound dressing.
2. preparation method according to claim 1, which is characterized in that the cross-linking reagent includes 1,2- dichloroethanes and N-
Hydroxysuccinimide.
3. preparation method according to claim 2, which is characterized in that the molar concentration of 1, the 2- dichloroethanes is
0.002-0.2M/mL, the molar concentration of the N- hydroxysuccinimide are 0.002-0.2M/mL；The crosslinking time be 2~
8h。
4. preparation method according to claim 1, which is characterized in that step A) quality of the gelatin in spinning solution
Concentration is 0.1~2g/mL；The additional amount of the chamomile essential oil is 20~100 μ l/mL；Acetic acid and water in the acetic acid solution
Mass ratio be (20~25): (1~5).
5. preparation method according to claim 1, which is characterized in that the electrospinning parameters specifically: spinneret voltage
For 8~21kV；Pump discharge is 0.2~2ml/h, and the distance between spinning head top and coverslip are 5~15cm.
6. a kind of nanometer of wound dressing, which is characterized in that the preparation method as described in Claims 1 to 5 any one is prepared into
It arrives.
7. a kind of preparation method of edible package, comprising:
A) by gelatin, acetic acid aqueous solution and chamomile essential oil stirring and dissolving, spinning solution is obtained；
B) by spinning solution electrostatic spinning, edible package is obtained.
8. preparation method according to claim 7, which is characterized in that step A) quality of the gelatin in spinning solution
Concentration is 0.1~2g/mL；The additional amount of the chamomile essential oil is 20~100 μ l/mL；Acetic acid and water in the acetic acid solution
Mass ratio be (20~25): (1~5).
9. preparation method according to claim 7, which is characterized in that the electrospinning parameters specifically: spinneret voltage
For 8~21kV；Pump discharge is 0.2~2ml/h, and the distance between spinning head top and coverslip are 5~15cm.
10. a kind of edible package, which is characterized in that the preparation method as described in claim 7~9 any one is prepared into
It arrives.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009179921A (en) * | 2008-02-01 | 2009-08-13 | Taiyo Kagaku Co Ltd | Polymerization type catechin nanofiber |
| CN104562438A (en) * | 2013-10-17 | 2015-04-29 | 中国科学院理化技术研究所 | Gelatin-based micro-nanofiber membrane material, preparation method and utilization thereof |
| CN105734830A (en) * | 2016-04-05 | 2016-07-06 | 东华大学 | Method for preparing composite nanofiber felt of houttuynia cordata essential oil and chitosan |
| CN106581779A (en) * | 2016-11-16 | 2017-04-26 | 华南理工大学 | Skin burn repair material and preparing method thereof |
| CN106676670A (en) * | 2016-12-09 | 2017-05-17 | 天长市天龙泵阀成套设备厂 | Hyaluronic acid/silk fibroin nano-fibre and preparation method thereof |
| CN106835504A (en) * | 2017-02-16 | 2017-06-13 | 江苏大学 | A kind of nanofiber antibacterial film and its production and use |
| CN106966047A (en) * | 2017-03-23 | 2017-07-21 | 浙江工业大学 | A kind of preparation method of edible nanofiber preservative film |
| CN107523940A (en) * | 2017-08-14 | 2017-12-29 | 江苏大学 | A kind of Preparation method and use of chu chrysanthemum essential oil/fibroin albumen antibacterial film |
| CN108866820A (en) * | 2017-05-12 | 2018-11-23 | 深圳瑞祥居科技发展有限公司 | A kind of preparation method and application of Electrospun nano-fibers |
-
2019
- 2019-01-10 CN CN201910022822.2A patent/CN109573322A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009179921A (en) * | 2008-02-01 | 2009-08-13 | Taiyo Kagaku Co Ltd | Polymerization type catechin nanofiber |
| CN104562438A (en) * | 2013-10-17 | 2015-04-29 | 中国科学院理化技术研究所 | Gelatin-based micro-nanofiber membrane material, preparation method and utilization thereof |
| CN105734830A (en) * | 2016-04-05 | 2016-07-06 | 东华大学 | Method for preparing composite nanofiber felt of houttuynia cordata essential oil and chitosan |
| CN106581779A (en) * | 2016-11-16 | 2017-04-26 | 华南理工大学 | Skin burn repair material and preparing method thereof |
| CN106676670A (en) * | 2016-12-09 | 2017-05-17 | 天长市天龙泵阀成套设备厂 | Hyaluronic acid/silk fibroin nano-fibre and preparation method thereof |
| CN106835504A (en) * | 2017-02-16 | 2017-06-13 | 江苏大学 | A kind of nanofiber antibacterial film and its production and use |
| CN106966047A (en) * | 2017-03-23 | 2017-07-21 | 浙江工业大学 | A kind of preparation method of edible nanofiber preservative film |
| CN108866820A (en) * | 2017-05-12 | 2018-11-23 | 深圳瑞祥居科技发展有限公司 | A kind of preparation method and application of Electrospun nano-fibers |
| CN107523940A (en) * | 2017-08-14 | 2017-12-29 | 江苏大学 | A kind of Preparation method and use of chu chrysanthemum essential oil/fibroin albumen antibacterial film |
Non-Patent Citations (2)
| Title |
|---|
| IOANNIS LIAKOS. ET AL.: "All-natural composite wound dressing films of essential oils encapsulated in sodium alginate with antimicrobial properties", 《INTERNATIONAL JOURNAL OF PHARMACEUTICS》 * |
| 朱庆棠等: "《周围神经缺损修复材料的生物制造与临床评估》", 31 August 2018, 中山大学出版社 * |
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