CN109557067A - One seed nucleus-satellite structure gold nano grain and preparation method thereof - Google Patents
One seed nucleus-satellite structure gold nano grain and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to nanomaterial sciences and analytical chemistry field, and in particular to one seed nucleus-satellite structure gold nano grain and preparation method thereof.The particle is made of three-decker, and for internal layer using single gold nanosphere as core, middle layer is to wrap up the phospholipid bilayer of gold nanosphere, and outermost layer is to be connected on phospholipid bilayer, around multiple " satellite " gold nanoparticles of gold nanosphere core.The preparation method is as follows: first preparing gold nanosphere core, liposome gold nanosphere is then used, finally restores trivalent complex ions of gold or monovalence complex ions of gold, growth in situ " satellite " gold nanoparticle on phospholipid bilayer with ascorbic acid.Preparation method is simple for satellite structure gold nano grain for the core-, the pattern and size tunable of product, stability and good dispersion.Due to the presence of hot spot between golden core-" satellite " particle, which there is good Surface enhanced Raman scattering (SERS) to enhance ability, have potential using value in analysis measurement field.
Description
Technical field
The present invention relates to nanomaterial sciences and analytical chemistry field, and in particular to one seed nucleus-satellite structure gold nano grain
And preparation method thereof.
Background technique
Surface Enhanced Raman Scattering Spectrum (SERS) refers to when Molecular Adsorption or is in close proximity to certain nanostructure
Metal surface when, the raman spectral signal of molecule is been significantly enhanced.The phenomenon is from 1974 by human hairs such as Fleischmann
After now, it is analyzed chemist's extensive concern always.SERS technology has its highly selective, highly sensitive and Fast nondestructive evaluation etc.
Advantage is dividing the fields such as biochemical analysis, biomedical engineering, environmental monitoring to illustrate important application potential.
Noble metal nano particles, which enhance substrate, structure and property as Raman signal, has decision to SERS signal intensity
Property influence.The study found that adjacent gold or Nano silver grain gap can significantly increase the analyte signal being located therein, it is this to receive
Rice gap is known as SERS " hot spot ".Noble metal nano particles with core-satellite (core-satellite) structure are a kind of heavy
The SERS underlying structure wanted passes through multiple nanometers outside link object connection by internal single nanogold or ping-pong ball (core)
Gold or silver particles (satellite) are combined.Between the core of the complex-satellite particle, between satellite-satellite particle all
It can be formed SERS " hot spot ", therefore there is good SERS to enhance performance.
It is the key that form the nanostructure that object is linked between core-satellite particle.Common link object has small molecule chemical combination
Object (alkanethiol, p-aminophenyl thiophenol, Streptavidin etc.) and macromolecular compound (mercapto-polyglycol, DNA, polyhistidyl
Deng).Inevitably cause some satellite particles that cannot link with core particle using small molecule link object, so as to cause satellite
The waste of particle；Often there is the problems such as composite particles low yield, architectural difference is big, and kernel-using macromolecular link object
It is spaced larger between satellite particle, is unfavorable for forming hot spot.Therefore, exploitation is novel, has the active core of high SERS-satellite knot
Structure noble metal nano particles are necessary.
Summary of the invention
It is an object of that present invention to provide one kind to have the active core of high SERS-satellite structure gold nano grain and its preparation
Method.
To achieve the above object, the invention adopts a technical scheme as:
One seed nucleus-satellite structure gold nano grain, the gold nano grain are made of three-decker, and diameter is 30-
260nm, internal layer are gold nanosphere core, and middle layer is to wrap up the phospholipid bilayer of gold nanosphere, and outermost layer is to be connected to phosphatide pair
Multiple " satellite " gold nanoparticles on layer.
The core gold nanosphere is that diameter is synthesized using seed mediated growth method in the gold nanosphere of 20-200nm；
Middle layer is with yolk phospholipid, soybean lecithin, dipalmitoylphosphatidylcholine (DPPC), two myristoyl lecithins
Rouge (DMPC), the phosphatide that the one or more of dipalmitoylphosphatidylethanolamine (DPPE) mix are raw material, are dispersed using film
Method obtains the liposome of 2-10mg/ml；
Outer layer is that the solution of gold ion is synthesized gold nanoparticle (diameter is 5-30nm) life in situ by reducing agent
Regular way is directly adsorbed and grafts on interlayer surfaces.
The core-satellite structure gold nano grain is to be with phosphatide by the gold nanosphere for using seed mediated growth method to synthesize
The liposome that raw material is obtained through film dispersion method is sufficiently mixed, and the phospholipid bilayer of liposome is made to be adsorbed on core gold nanosphere
Surface then adds the solution containing gold ion and is directly grafted on by reducing agent synthesis gold nanoparticle in situ synthesis
It states on the phospholipid bilayer on package gold nanosphere surface to get core-satellite structure gold nano grain.
The preparation method of one seed nucleus-satellite structure gold nano grain:
1) use seed mediated growth method synthesis diameter for 20-200nm gold nano cores；
2) liposome of 2-10mg/ml is obtained through film dispersion method using phosphatide as raw material, liposomal diameter is 50-
500nm；
3) after being sufficiently mixed the excessive liposome of step 2) and the gold nano core of step 1), keep the phosphatide of liposome double
Molecular layer adsorption is on core gold nanosphere surface；
4) it takes step 3) to obtain the gold nano core of phospholipid bilayer package, it is added under gentle agitation 1/50 to isometric anti-
Bad hematic acid makes the final concentration of 0.1M -1M of ascorbic acid in system, adds and contains gold ion with ascorbic acid same volume
Solution, make the final concentration of 0.5mM -10mM of the solution containing gold ion in system, and then the multiple gold nanoparticles synthesized
(diameter be 5-30nm) make its situ-formed graft on the phospholipid bilayer on above-mentioned package gold nanosphere surface to get core-
Satellite structure gold nano grain.
The solution containing gold ion is to include trivalent complex ions of gold (AuCl4 -Or AuBr4 -) gold chloride, chlorine gold
Sour sodium, potassium chloroaurate, auribromohydric acid, sodium bromaurate, potassium auric bromide include monovalence complex ions of gold (AuCl2 -Or AuBr2 -) dichloro
Change gold chloride, dichloride sodium chloraurate, dichloride potassium chloroaurate, dibrominated auribromohydric acid, dibrominated sodium bromaurate, dibrominated bromine gold
Sour potassium.
The application of one seed nucleus-satellite structure gold nano grain, the core-satellite structure gold nano grain are received as SERS
Application of the rice probe in biomarker.
Beneficial effects of the present invention:
1. the present invention is using phospholipid bilayer as link object, " satellite " gold particle of growth in situ all be can connect
On phospholipid bilayer, the partial size and pattern of synthetic product are uniform, good dispersion.
2. the thickness of phospholipid bilayer of the present invention is about 5nm, ensure that between inner core and outside " satellite " gold particle
The presence of hot spot, and the density of external " satellite " gold particle is high, and gap can also form hot spot, to make the SERS of the structure
Activity is significantly improved.
3. the present invention selects phosphatide as the link object between forming core-satellite nanoparticle, make product that there is good life
Object compatibility has biological medicine application potential.
4. particle synthesis process of the present invention is easy, no coupling product, it is convenient for large-scale production.
Detailed description of the invention
Fig. 1 is acquisition core-satellite structure gold nano grain schematic diagram that the embodiment of the present invention mentions.
Fig. 2 is the golden core of core-satellite structure internal layer 80nm that the embodiment of the present invention mentions, and is prepared with chlorauric acid solution
Obtained core-satellite structure gold nano grain scanning electron microscope (SEM) photograph.
The 80nm gold goal for the respectively phospholipid bilayer package that Fig. 3 mentions for the embodiment of the present invention is prepared using chlorauric acid solution
Obtained core-satellite structure gold nano grain schemes the SERS of malachite green isothiocyanate.
The 80nm gold goal for the respectively phospholipid bilayer package that Fig. 4 mentions for the embodiment of the present invention is prepared using chlorauric acid solution
Obtained core-satellite structure gold nano grain schemes the SERS of crystal violet.
Fig. 5 is the core being prepared with dibrominated sodium bromaurate solution-satellite structure gold nano that the embodiment of the present invention mentions
The scanning electron microscope (SEM) photograph of particle.
Fig. 6 is the 80nm gold goal for the respectively phospholipid bilayer package that the embodiment of the present invention mentions, using dibrominated sodium bromaurate
The core that solution is prepared-satellite structure gold nano grain schemes the SERS of malachite green isothiocyanate.
Fig. 7 is the 80nm gold goal for the respectively phospholipid bilayer package that the embodiment of the present invention mentions, using dibrominated sodium bromaurate
The core that solution is prepared-satellite structure gold nano grain schemes the SERS of crystal violet.
Fig. 8 uses the gold goal of 23nm as internal layer gold core for what the embodiment of the present invention mentioned, the core being prepared-satellite knot
The scanning electron microscope (SEM) photograph of structure gold nano grain.
Fig. 9 uses dipalmitoylphosphatidylcholine (DPPC) as the phosphatide material of middle layer for what the embodiment of the present invention mentioned
Material, the core being prepared-satellite structure gold nano grain scanning electron microscope (SEM) photograph.
Specific embodiment:
Implementation will the present invention will be further described in conjunction with attached drawing below.
Embodiment 1
It is link object with egg yolk lecithin using 80nm gold goal as core, generates " satellite " gold using gold chloride in-situ reducing
Nanoparticle prepares core-satellite structure gold nano grain (referring to Fig. 1):
(1) preparation of gold nanosphere core:
The preparation of 80nm gold goal core: taking the gold chloride (50mM) of 50 μ l to be dissolved in 4.95ml pure water first, and 5ml ten is added
Six alkyl trimethyl ammonium bromides (0.2M) at 27 DEG C, are added iced (the 0 DEG C or less) 0.01M's of 600 μ l after mixing evenly
Sodium borohydride reacts 15min, and after standing 3h, gold seeds are made；Then, by 2ml hexadecyltrimethylammonium chloride (0.2M),
1.5ml ascorbic acid (0.1M), the above-mentioned gold seeds solution of 50 μ l are thoroughly mixed, in 27 DEG C of addition 2ml gold chlorides (0.5mM)
React 15min, by above-mentioned product 12000rpm eccentric cleaning it is secondary after, be concentrated in 1ml hexadecyltrimethylammonium chloride
In (0.02M), 10nm gold core solution is obtained.By 40ml hexadecyltrimethylammonium chloride (0.1M), 2.6ml ascorbic acid
(0.01M), the above-mentioned 10nm gold core solution of 100 μ l is thoroughly mixed, and at 27 DEG C, is added with syringe pump with the rate of 40ml/h
The gold chloride (0.5mM) of 40ml, the reaction was continued 15min after being added dropwise, obtains 80nm gold goal core, for use.
(2) preparation of liposome: taking yolk phospholipid in a round bottom flask, and dehydrated alcohol mixing is added, makes egg in mixed liquor
The final concentration of 5mg/ml of yellow phosphorus rouge.After being completely dissolved, 37 DEG C of revolvings remove ethyl alcohol, make to form film, vacuum on round-bottomed flask wall
Dry 2h.Adding pure water hydrated films , Zhen Oscillating identical with dehydrated alcohol amount is completely dissolved the film in bottle wall, obtains muddy
Turbid white solution, then ultrasound is until solution clear, crosses 0.22 μm of filter membrane 3 times for resulting clear solution.
(3) preparation of the gold nanosphere of phospholipid bilayer package: after taking 80nm gold goal core 1ml, 3000rpm to be centrifuged 10min
Supernatant is sucked, bottom precipitation is dispersed with 1ml liposome solutions, and after left at room temperature over night, 3000rpm is centrifuged 10min, is sucked
Clear liquid.Bottom precipitation with 50 μ l pure water disperse after, be added dropwise in 1ml liposome, at 50 DEG C stir 4h after, 3000rpm from
Heart 10min, sucks supernatant, is dispersed with 1ml pure water, realizes that the phospholipid bilayer of liposome is adsorbed on core gold nanosphere table
Face.
(4) core-satellite structure gold particle preparation: (concentration is adjusted to maximum to the gold nanosphere 1ml for taking phospholipid bilayer to wrap up
Absorbance is the gold chloride for 1.0), under gentle agitation sequentially adding the ascorbic acid (0.1M) and 100 μ l of 100 μ l at absorbing wavelength
(2.5mM) makes many gold nanoparticles to be formed (5-30nm of diameter) situ-formed graft in above-mentioned package gold nanosphere table
To get core-satellite structure gold nano grain (referring to fig. 2) on the phospholipid bilayer in face.
From Figure 2 it can be seen that A indicates that internal layer 80nm gold core, B indicate the core-satellite structure gold particle prepared using gold chloride,
It can be clearly seen that the outer surface of internal gold goal core has linked gold nano grain, and core-satellite structure is uniform, disperse
Property is good.
(5) core-satellite structure gold nano grain Raman signal enhancing ability is investigated: 1ml phospholipid bilayer being taken to wrap up
Core-satellite structure gold particle that 80nm gold goal, 1ml use gold chloride to prepare, they are respectively added 10 respectively—6The Raman report of M
Molecule malachite green isothiocyanate and crystal violet are accused, is 4mw in laser intensity, optical maser wavelength measures under conditions of being 633nm
Their Raman signal intensity (referring to Fig. 3 and Fig. 4).
As seen from Figure 3 be malachite green isothiocyanate Raman spectrogram, Fig. 4 be crystal violet Raman spectrogram, two
A and B is respectively indicated using the 80nm gold goal of phospholipid bilayer package and core-satellite structure gold particle as strengthening material in a figure
Material.It can be seen that core-satellite structure gold particle that the present invention synthesizes is bright for the SERS enhancing ability of above two report molecule
It is aobvious to be better than gold goal.
Embodiment 2
Using 80nm gold goal as core, it is link object with egg yolk lecithin, is generated using dibrominated sodium bromaurate in-situ reducing
" satellite " gold nanoparticle prepares core-satellite structure gold nano grain, and difference from Example 1 is:
The preparation of dibrominated sodium bromaurate solution: weighing 0.2g sodium bromide and be dissolved in 3ml pure water and obtain sodium bromide solution,
120 μ l gold chlorides (50mM) are added in 1.98ml pure water, are added with stirring the 300 above-mentioned sodium bromide solutions of μ l, are finally adding dropwise
Enter 0.1M ascorbic acid and become colourless by buff to solution to can be obtained dibrominated sodium bromaurate solution；
The preparation of core-satellite structure gold particle: (concentration is adjusted to absorption maximum to the gold nanosphere 1ml for taking phospholipid bilayer to wrap up
Absorbance is the above-mentioned dibrominated for 1.0), under gentle agitation sequentially adding the ascorbic acid (0.1M) and 100 μ l of 100 μ l at wavelength
Sodium bromaurate solution (2.5mM) makes many gold nanoparticles to be formed (5-30nm of diameter) situ-formed graft in above-mentioned packet
It wraps up on the phospholipid bilayer on gold nanosphere surface to get core-satellite structure gold nano grain (referring to Fig. 5).
By in Fig. 5, it can be seen that the outer surface of internal gold goal has linked gold nano grain, and core-satellite structure is equal
It is even, good dispersion.
Core-satellite structure gold nano grain Raman signal enhancing ability is investigated: the 80nm for taking 1ml phospholipid bilayer to wrap up
Core-satellite structure gold particle that gold goal, 1ml are prepared using dibrominated sodium bromaurate, they are respectively added 10 respectively—6The drawing of M
Graceful report molecule malachite green isothiocyanate and crystal violet are 4mw in laser intensity, under conditions of optical maser wavelength is 633nm
Measure their Raman signal intensity (referring to figure Fig. 6 and 7).
It is respectively the Raman spectrogram of malachite green isothiocyanate and crystal violet, A and B in two figures referring to Fig. 6 and Fig. 7
It respectively indicates using the 80nm gold goal of phospholipid bilayer package and core-satellite structure gold particle as reinforcing material.It can be seen that
The core that the present invention synthesizes-satellite structure gold particle is substantially better than gold goal for the SERS enhancing ability of above two report molecule.
Embodiment 3
It is link object with egg yolk lecithin using 23nm gold goal as core, generates " satellite " gold using gold chloride in-situ reducing
Nanoparticle prepares core-satellite structure gold nano grain:
(1) preparation of 23nm gold goal core: taking the gold chloride (50mM) of 50 μ l to be dissolved in 4.95ml pure water first, is added
At 27 DEG C, 600 μ l iced (0 DEG C or less) is added after mixing evenly in 5ml cetyl trimethylammonium bromide (0.2M)
The sodium borohydride of 0.01M reacts 15min, and after standing 3h, gold seeds solution is made；Then, by 2ml cetyl trimethyl chlorination
Ammonium (0.2M), 1.5ml ascorbic acid (0.1M), the above-mentioned gold seeds solution of 50 μ l are thoroughly mixed, in 27 DEG C of addition 2ml chlorine gold
Sour (0.5mM) reacts 15min, by above-mentioned product 12000rpm eccentric cleaning it is secondary after, be concentrated in 1ml cetyl trimethyl chlorine
Change in ammonium (0.02M), obtains 10nm gold core solution.By 40ml hexadecyltrimethylammonium chloride (0.1M), 2.6ml ascorbic acid
(0.01M), the above-mentioned 10nm gold core of 2ml is thoroughly mixed, and at 27 DEG C, is added 40ml's with syringe pump with the rate of 40ml/h
Gold chloride (0.5mM), the reaction was continued 15min after being added dropwise, obtains 23nm gold goal core, for use.
(2) preparation of liposome: preparation method is the same as (2) in embodiment 1.
(3) preparation of the gold nanosphere of phospholipid bilayer package: after taking 23nm gold goal core 1ml, 10000rpm to be centrifuged 10min
Supernatant is sucked, bottom precipitation is dispersed with 1ml liposome solutions, and after left at room temperature over night, 10000rpm is centrifuged 10min, is sucked
Supernatant.After bottom precipitation is dispersed with 50 μ l pure water, it is added dropwise in 1ml liposome, after stirring 4h at 50 DEG C, 10000rpm
It is centrifuged 10min, sucks supernatant, is dispersed with 1ml pure water, realizes that the phospholipid bilayer of liposome is adsorbed on core gold nanosphere
Surface.
(4) core-satellite structure gold particle preparation: (concentration is adjusted to maximum to the gold nanosphere 1ml for taking phospholipid bilayer to wrap up
Absorbance is the gold chloride for 1.0), under gentle agitation sequentially adding the ascorbic acid (0.1M) and 100 μ l of 100 μ l at absorbing wavelength
(2.5mM) makes many gold nanoparticles to be formed (5-30nm of diameter) situ-formed graft in above-mentioned package gold nanosphere table
To get core-satellite structure gold nano grain on the phospholipid bilayer in face (referring to Fig. 8).
Gold nano grain, and the partial size and pattern of synthetic product have also been linked outside the golden core of internal layer as seen from Figure 8
More uniform, preferably, i.e. the size of change internal layer core can also synthesize the core-satellite structure gold particle to dispersibility.
Embodiment 4
It is link object with dipalmitoylphosphatidylcholine (DPPC) using 80nm gold goal as core, is gone back in situ using gold chloride
Primary to prepare core-satellite structure gold nano grain at " satellite " gold nanoparticle, difference from Example 1 is:
The preparation of liposome: taking DPPC in a round bottom flask, and dehydrated alcohol mixing is added, makes DPPC final concentration in mixed liquor
For 5mg/ml.After being completely dissolved, 37 DEG C of revolvings remove ethyl alcohol, make to form film on round-bottomed flask wall, are dried in vacuo 2h.It adds
Pure water hydrated films , Zhen Oscillating identical with dehydrated alcohol amount is completely dissolved the film in bottle wall, obtains muddy white solution,
Then ultrasound is until solution clear.Resulting clear solution is crossed into 0.22 μm of filter membrane 3 times.
The preparation of the gold nanosphere of phospholipid bilayer package: 80nm gold goal core 1ml is taken, is sucked after 3000rpm centrifugation 10min
Supernatant, bottom precipitation are dispersed with 1ml liposome solutions, and after standing overnight at 50 DEG C, 3000rpm is centrifuged 10min, suck supernatant
Liquid.After bottom precipitation is dispersed with 50 μ l pure water, it is added dropwise in 1ml liposome, after stirring 4h at 50 DEG C, 3000rpm centrifugation
10min sucks supernatant, is dispersed with 1ml pure water, realizes that the phospholipid bilayer of liposome is adsorbed on core gold nanosphere table
Face.
The preparation of core-satellite structure gold particle: (concentration is adjusted to absorption maximum to the gold nanosphere 1ml for taking phospholipid bilayer to wrap up
Absorbance is the gold chloride for 1.0), under gentle agitation sequentially adding the ascorbic acid (0.1M) and 100 μ l of 100 μ l at wavelength
(2.5mM) makes many gold nanoparticles to be formed (5-30nm of diameter) situ-formed graft in above-mentioned package gold nanosphere table
To get core-satellite structure gold nano grain on the phospholipid bilayer in face (referring to Fig. 9).
It is the visible core prepared by phospholipid material of DPPC-satellite structure gold particle by Fig. 9, golden core external linkage
Gold nano grain, the partial size and pattern of product are uniform, and dispersibility is preferably.The type for changing intermediate phospholipid bilayer is demonstrated, it can also
Successfully to synthesize the core-satellite structure gold particle.
Embodiment 5
Using 150nm gold goal as core, it is link object with distearyl acyl group lecithin (DSPC), utilizes Au3+In-situ reducing is raw
At " satellite " gold nanoparticle, core-satellite structure gold nano grain is prepared:
(1) preparation of 150nm gold goal: taking the gold chloride (50mM) of 50 μ l to be dissolved in 4.95ml pure water first, and 5ml ten is added
Six alkyl trimethyl ammonium bromides (0.2M) at 27 DEG C, are added iced (the 0 DEG C or less) 0.01M's of 600 μ l after mixing evenly
Sodium borohydride reacts 15min, and after standing 3h, gold seeds are made；Then, by 2ml hexadecyltrimethylammonium chloride (0.2M),
1.5ml ascorbic acid (0.1M), the above-mentioned gold seeds solution of 50 μ l are thoroughly mixed, in 27 DEG C of addition 2ml gold chlorides (0.5mM)
React 15min, by above-mentioned product 12000rpm eccentric cleaning it is secondary after, be concentrated in 1ml hexadecyltrimethylammonium chloride
In (0.02M), 10nm gold core solution is obtained；By 2ml hexadecyltrimethylammonium chloride (0.1M), 130ul ascorbic acid
(0.01M), the above-mentioned 10nm gold core of 10 μ l is thoroughly mixed, and at 27 DEG C, is added 2ml's with syringe pump with the rate of 2ml/h
Gold chloride (0.5mM), after being added dropwise after the reaction was continued 15min, the pure water after 6000rpm eccentric cleaning is secondary, with 0.86ml
Mixing, obtains the gold nanosphere of 46nm；By 2ml hexadecyltrimethylammonium chloride (0.1M), 130ul ascorbic acid (0.01M),
The above-mentioned 46nm gold core of 50 μ l is thoroughly mixed, and at 27 DEG C, the gold chloride of 2ml is added with the rate of 2ml/h with syringe pump
Rear the reaction was continued 15min is added dropwise to get the gold goal of 150nm in (0.5mM).
(2) preparation of liposome: weighing a certain amount of DSPC in a round bottom flask, and dehydrated alcohol, which is added, makes its concentration
5mg/ml.After being completely dissolved, 37 DEG C of revolvings remove ethyl alcohol, make to form film on round-bottomed flask wall, are dried in vacuo 2h.Add with
The identical pure water hydrated films , Zhen Oscillating of amount of alcohol is completely dissolved the film in bottle wall, obtains muddy white solution, then surpasses
Sound is until solution clear.Resulting clear solution is crossed into 0.22 μm of filter membrane 3 times.
(3) preparation of the gold nanosphere of phospholipid bilayer package: 80nm gold goal 1ml is taken, is sucked after 3000rpm centrifugation 10min
Supernatant, bottom precipitation are dispersed with 1ml liposome solutions, and after standing overnight at 60 DEG C, 3000rpm is centrifuged 10min, suck supernatant
Liquid.After bottom precipitation is dispersed with 50 μ l pure water, it is added dropwise in 1ml liposome, after stirring 4h at 60 DEG C, 3000rpm centrifugation
10min sucks supernatant, is dispersed with 1ml pure water.
(4) core-satellite structure gold particle preparation: (concentration is adjusted to maximum to the gold nanosphere 1ml for taking phospholipid bilayer to wrap up
Absorbance is the gold chloride for 1.0), under gentle agitation sequentially adding the ascorbic acid (0.5M) and 20 μ l of 20 μ l at absorbing wavelength
(12.5mM)。
Above-described embodiment only technical concept and feature to illustrate the invention, its object is to allow person skilled in the art
Scholar cans understand the content of the present invention and implement it accordingly, and can not limit the scope of the invention according to this.It is all according to the present invention
Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.
Claims (6)
1. one seed nucleus-satellite structure gold nano grain, it is characterised in that: the gold nano grain is made of three-decker, diameter
For 30-260nm, internal layer is gold nanosphere core, and middle layer is to wrap up the phospholipid bilayer of gold nanosphere, and outermost layer is to be connected to
Multiple " satellite " gold nanoparticles on phospholipid bilayer.
2. core according to claim 1-satellite structure gold nano grain, it is characterised in that: the core gold nanosphere is
Diameter is synthesized in the gold nanosphere of 20-200nm using seed mediated growth method；
Middle layer is with yolk phospholipid, soybean lecithin, dipalmitoylphosphatidylcholine (DPPC), two myristoyl lecithin
(DMPC), the phosphatide of one or more of mixing of dipalmitoylphosphatidylethanolamine (DPPE) is raw material, using film dispersion method
Obtain the liposome of 2-10mg/ml；
Outer layer is directly to adsorb the solution of gold ion by reducing agent synthesis gold nanoparticle in situ synthesis to graft on centre
Layer surface.
3. core according to claim 1 or 2-satellite structure gold nano grain, it is characterised in that: the core-satellite knot
Structure gold nano grain is to obtain by the gold nanosphere for using seed mediated growth method to synthesize and by raw material of phosphatide through film dispersion method
Liposome is sufficiently mixed, and the phospholipid bilayer of liposome is made to be adsorbed on core gold nanosphere surface, is then added containing gold
The solution of ion synthesizes gold nanoparticle in situ synthesis by reducing agent and directly grafts on above-mentioned package gold nanosphere surface
To get core-satellite structure gold nano grain on phospholipid bilayer.
4. a kind of preparation method of core described in claim 1-satellite structure gold nano grain, it is characterised in that:
1) use seed mediated growth method synthesis diameter for 20-100nm gold nano cores；
2) liposome of 2-10mg/ml is obtained through film dispersion method using phosphatide as raw material, liposomal diameter is 50-500nm；
3) after being sufficiently mixed the excessive liposome of step 2) and the gold nano core of step 1), make the phospholipid bilayer of liposome
Layer is adsorbed on core gold nanosphere surface；
4) it takes step 3) to obtain the gold nano core of phospholipid bilayer package, it is added under gentle agitation 1/50 to isometric Vitamin C
Acid, and the solution containing gold ion with ascorbic acid same volume make the gold nanoparticle situ-formed graft of synthesis in above-mentioned packet
It wraps up on the phospholipid bilayer on gold nanosphere surface to get core-satellite structure gold nano grain.
5. core according to claim 4-satellite structure gold nano grain, it is characterised in that: described to contain the molten of gold ion
Liquid is to include trivalent complex ions of gold (AuCl4 -Or AuBr4 -) gold chloride, sodium chloraurate, potassium chloroaurate, auribromohydric acid, auribromohydric acid
Sodium, potassium auric bromide include monovalence complex ions of gold (AuCl2 -Or AuBr2 -) dichloride gold chloride, dichloride sodium chloraurate, dichloro
Change potassium chloroaurate, dibrominated auribromohydric acid, dibrominated sodium bromaurate, dibrominated potassium auric bromide.
6. a kind of application of core described in claim 1-satellite structure gold nano grain, it is characterised in that: the core-satellite
Application of the structure gold nano grain as SERS nano-probe in biomarker.
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