CN109554436A - A method of utilizing microorganism monitoring water pollution degree - Google Patents
A method of utilizing microorganism monitoring water pollution degree Download PDFInfo
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- CN109554436A CN109554436A CN201811415096.2A CN201811415096A CN109554436A CN 109554436 A CN109554436 A CN 109554436A CN 201811415096 A CN201811415096 A CN 201811415096A CN 109554436 A CN109554436 A CN 109554436A
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- minimal medium
- microorganism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of methods using microorganism monitoring water pollution degree, are related to water quality inspection technique field, comprising the following steps: S1, microorganism is linked into full nutrient medium and cultivates；S2, the full nutrient medium for being connected to microorganism is extracted, minimal medium is added in taking precipitate, and extraction takes precipitating that minimal medium is added, obtains mixed liquor；S3, given the test agent is diluted to as preparation liquid by various concentration using minimal medium；S4, mixed liquor is added separately in preparation liquid and blank minimal medium, observation counts；S5, the result judgement provisional value according to S4, the preparation liquid that 1%~100% preliminary concentration is prepared using minimal medium dilution given the test agent, mixed liquor is added separately in preparation liquid and blank minimal medium, observation counts, significant difference comparison is carried out, judges given the test agent bio-toxicity.The method of the present invention is easy, low in cost, is capable of the bio-toxicity of effective evaluation given the test agent, can be used for the Toxicity Monitoring of water quality or chemical substance.
Description
Technical field
The present invention relates to water quality monitoring technical field more particularly to a kind of sides using microorganism monitoring water pollution degree
Method.
Background technique
Social water pollution problems is increasingly severe now, has influenced human lives and the ecological balance, drinking water safety
Also become one of the problem of people increasingly pay close attention to, therefore, many scholars are dedicated to the research of water quality monitoring method.Existing side
Method includes physico-chemical analysis method, and this method speed is slow, and operation is comparatively laborious, and accuracy is not also high.
Summary of the invention
Technical problems based on background technology utilize microorganism monitoring water pollution degree the invention proposes a kind of
Method.
A kind of method using microorganism monitoring water pollution degree proposed by the present invention, comprising the following steps:
S1, microorganism is linked into full nutrient medium and is cultivated to stationary phase；
S2, the full nutrient medium for being connected to microorganism is extracted, discards supernatant liquid, inorganic salts culture is added in taking precipitate
Base cleans, and extraction takes precipitating that minimal medium is added, obtains mixed liquor；
S3, it given the test agent is diluted to as preparation liquid by various concentration using minimal medium acts on microorganism；
S4, mixed liquor is added separately in the preparation liquid and blank minimal medium of various concentration, preparation liquid conduct
Test group, blank minimal medium as a control group, count microorganism using micro- sem observation；
S5, the result judgement 72h half lethal concentration provisional value according to S4 dilute given the test agent using minimal medium
Mixed liquor is added separately to the preparation liquid of the various concentration of this step preparation by the preparation liquid for preparing 1%~100% preliminary concentration
With carry out exposure test in blank minimal medium, preparation liquid as test group, blank minimal medium as a control group,
Microorganism is counted using micro- sem observation, significant difference comparison is carried out, judges given the test agent bio-toxicity.
The beneficial effect generated by adopting the above technical scheme is: the present invention select microorganism carry out acute toxicity test and
Chronic toxicity test carrys out fast slowdown monitoring water quality, and method is easy, low in cost, is capable of the bio-toxicity of effective evaluation given the test agent,
It can be used for the Toxicity Monitoring of water quality or chemical substance.
Specific embodiment
A method of utilizing microorganism monitoring water pollution degree, comprising the following steps:
S1, microorganism is linked into full nutrient medium and is cultivated to stationary phase；
S2, the full nutrient medium for being connected to microorganism is extracted, discards supernatant liquid, inorganic salts culture is added in taking precipitate
Base cleans, and extraction takes precipitating that minimal medium is added, obtains mixed liquor；
S3, it given the test agent is diluted to as preparation liquid by various concentration using minimal medium acts on microorganism；
S4, mixed liquor is added separately in the preparation liquid and blank minimal medium of various concentration, preparation liquid conduct
Test group, blank minimal medium as a control group, count microorganism using micro- sem observation；
S5, the result judgement 72h half lethal concentration provisional value according to S4 dilute given the test agent using minimal medium
Mixed liquor is added separately to the preparation liquid of the various concentration of this step preparation by the preparation liquid for preparing 1%~100% preliminary concentration
With carry out exposure test in blank minimal medium, preparation liquid as test group, blank minimal medium as a control group,
Microorganism is counted using micro- sem observation, significant difference comparison is carried out, judges given the test agent bio-toxicity.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (1)
1. a kind of method using microorganism monitoring water pollution degree, which comprises the following steps:
S1, microorganism is linked into full nutrient medium and is cultivated to stationary phase；
S2, the full nutrient medium for being connected to microorganism is extracted, discards supernatant liquid, minimal medium is added in taking precipitate, clearly
It washes, extracts, take precipitating that minimal medium is added, obtain mixed liquor；
S3, it given the test agent is diluted to as preparation liquid by various concentration using minimal medium acts on microorganism；
S4, mixed liquor is added separately in the preparation liquid and blank minimal medium of various concentration, preparation liquid is as test
Group, blank minimal medium as a control group, count microorganism using micro- sem observation；
S5, the result judgement 72h half lethal concentration provisional value according to S4 are prepared using minimal medium dilution given the test agent
Mixed liquor is added separately to the preparation liquid and sky of the various concentration of this step preparation by the preparation liquid of 1%~100% preliminary concentration
Exposure test is carried out in white minimal medium, preparation liquid as a control group, is used as test group, blank minimal medium
Micro- sem observation counts microorganism, carries out significant difference comparison, judges given the test agent bio-toxicity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811415096.2A CN109554436A (en) | 2018-11-26 | 2018-11-26 | A method of utilizing microorganism monitoring water pollution degree |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811415096.2A CN109554436A (en) | 2018-11-26 | 2018-11-26 | A method of utilizing microorganism monitoring water pollution degree |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109554436A true CN109554436A (en) | 2019-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811415096.2A Withdrawn CN109554436A (en) | 2018-11-26 | 2018-11-26 | A method of utilizing microorganism monitoring water pollution degree |
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| CN (1) | CN109554436A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308255A (en) * | 2019-05-08 | 2019-10-08 | 宁波大学 | One kind is based on Pollution indicating bacteria group to coastal waters degree of water pollution quantitative forecasting technique |
-
2018
- 2018-11-26 CN CN201811415096.2A patent/CN109554436A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110308255A (en) * | 2019-05-08 | 2019-10-08 | 宁波大学 | One kind is based on Pollution indicating bacteria group to coastal waters degree of water pollution quantitative forecasting technique |
| CN110308255B (en) * | 2019-05-08 | 2021-11-02 | 宁波大学 | Pollution indication flora based method for quantitatively predicting pollution degree of offshore water body |
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| WW01 | Invention patent application withdrawn after publication | ||
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Application publication date: 20190402 |