CN109553695A - Utilize the method for the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction - Google Patents
Utilize the method for the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction Download PDFInfo
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- CN109553695A CN109553695A CN201811468340.1A CN201811468340A CN109553695A CN 109553695 A CN109553695 A CN 109553695A CN 201811468340 A CN201811468340 A CN 201811468340A CN 109553695 A CN109553695 A CN 109553695A
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 37
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 37
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000000605 extraction Methods 0.000 title claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000000843 powder Substances 0.000 claims abstract description 21
- 239000008367 deionised water Substances 0.000 claims abstract description 15
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 15
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000001035 drying Methods 0.000 claims abstract description 12
- 239000012141 concentrate Substances 0.000 claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 239000007787 solid Substances 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 230000001376 precipitating effect Effects 0.000 claims abstract description 7
- 229960000935 dehydrated alcohol Drugs 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 5
- 238000000527 sonication Methods 0.000 claims description 2
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 3
- 238000000926 separation method Methods 0.000 abstract 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 7
- 239000000284 extract Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 1
- 241000222485 Agaricales Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 240000000588 Hericium erinaceus Species 0.000 description 1
- 235000007328 Hericium erinaceus Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000722337 Pholiota Species 0.000 description 1
- 244000252132 Pleurotus eryngii Species 0.000 description 1
- 235000001681 Pleurotus eryngii Nutrition 0.000 description 1
- 240000006079 Schisandra chinensis Species 0.000 description 1
- 235000008422 Schisandra chinensis Nutrition 0.000 description 1
- 241000123672 Strophariaceae Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000004146 energy storage Methods 0.000 description 1
- 238000011436 enzymatic extraction method Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Utilize the method for the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction, comprising the following steps: take autochthonal squama adiposa mycelium, 12h is dried under the conditions of 65 DEG C to constant weight；Then it crushes and sieves with 100 mesh sieve to obtain bacterium powder；Deionized water is added in autochthonal squama adiposa mycelium powder using ultrasonic wave auxiliary law to be extracted, dosage is that 20-40ml deionized water is added in every gram of solid powder, and water temperature is maintained at 60-80 DEG C, ultrasonic power 600-800w, ultrasonic time is 10-30min, is repeated 3 times；Extracting solution centrifuge separation is taken into supernatant, is then concentrated under reduced pressure, then dehydrated alcohol is added in concentrate, is then stood at 4 DEG C for 24 hours, centrifuging and taking precipitating；Gained precipitating successively washed once with acetone, ether, then drying to constant weight at 60 DEG C to get autochthonal squama adiposa mycelium polysaccharide.This method polysaccharide yield is high, and step is simple, and preparation cost is low.
Description
Technical field
The method that the present invention relates to the use of the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction.
Background technique
Autochthonal squama umbrella is under the jurisdiction of Basidiomycota, agaric guiding principle, Agaricales, Strophariaceae, Pholiota.It is distributed Jilin, Tibet, blueness
Sea, Sichuan, Yunnan, Japan and North America are also distributed.Polysaccharide is the most abundant a kind of biopolymer of content in nature,
Have the function of that energy storage, structural support, defence and antigen are decisive etc..To the extracting mode of Mushroom polysaccharide, the most commonly used is hot water
Extraction, Enzymatic Extraction method, microwave loss mechanisms and ultrasonic wave assisted extraction method etc..It is a large amount of research shows that: mushroom class nutrition is rich
Richness containing polysaccharide, amino acid, crude fat, and contains multi mineral prime element.Mushroom Mushroom polysaccharide has anti-oxidant, antiviral, suppression
The effects of bacterium reduces cholesterol, inhibits the growth of tumour, improves body's immunity and improves eyesight, improve endurance.
Polysaccharide comes from the natural macromolecular substance of high animal and plant cells film, microorganism wall, is all life
The important composition ingredient of body and the necessary structural material that sustains life.Polysaccharide can improve the edible quality of food, processing spy
Property and appearance characteristics, can be used for inhibiting lipid oxidation, the effect of stable acidic beverage also can be used as emulsifier etc..In food
Purposes it is very extensive.It is its widely applied basis to which the extraction of polysaccharide becomes particularly critical.Rare mushroom class is to grind now
The hot spot studied carefully, the such as autochthonal squama umbrella of various rare mushroom classes, sliding mushroom, Pleurotus eryngii, Hericium erinaceus etc. are containing having high nutrition for human body
The protein of various high-contents of value, polysaccharide, vitamin and mineral and low content fat.
The prior art to the extraction of autochthonal squama adiposa mycelium polysaccharide the problem is that, the yield of polysaccharide is lower, preparation step
It is rapid complicated, higher cost.
Summary of the invention
It is high that technical problem to be solved by the invention is to provide a kind of polysaccharide yields, and step is simple, and utilization at low cost is super
The method of the autochthonal squama adiposa mycelium polysaccharide of sound wave assisted extraction.
The present invention provides utilize the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction to solve above-mentioned technical problem
Method, comprising the following steps:
(1) autochthonal squama adiposa mycelium is taken, 12h is dried under the conditions of 65 DEG C to constant weight；
(2) the autochthonal squama adiposa mycelium after step (1) drying is crushed at 12000rpm with high speed Universal pulverizer
Then 1min sieves with 100 mesh sieve to obtain bacterium powder；
(3) deionized water is added in autochthonal squama adiposa mycelium powder using ultrasonic wave auxiliary law to be extracted, dosage is
20-40ml deionized water is added in every gram of solid powder, and water temperature is maintained at 60-80 DEG C, ultrasonic power 600-800w, ultrasonic time
For 10-30min, repeats extraction 3 times and obtain extracting solution；
(4) extracting solution is centrifugated, centrifugal rotational speed 5000rpm, centrifugation time 15min takes supernatant, then will
Supernatant liquor is concentrated under reduced pressure into 2/3rds formation concentrates of original volume at 50 DEG C, then anhydrous second is added in concentrate
Alcohol, the volume of dehydrated alcohol are three times of the volume of the concentrated liquid, are then stood at 4 DEG C for 24 hours, and then centrifuging and taking precipitates again, centrifugation
Revolving speed is 5000rmp, centrifugation time 15min；
(5) gained precipitating successively washed once with acetone, ether, then drying to constant weight at 60 DEG C to get autochthonal
Squama adiposa mycelium polysaccharide.
For the sake of simple declaration problem, the autochthonal squama adiposa mycelium of ultrasonic wave assisted extraction is utilized to of the present invention below
The method of polysaccharide is referred to as this method.
The advantages of this method: it is high as raw material extraction polysaccharide yield using autochthonal squama agaric by this method, and without using
Expensive instrument or other special additives, operating procedure is simple, and preparation cost is low, is suitable for that large-scale promotion uses.
In order to reach the better extraction effect of this method, preferred embodiment is as follows:
Preferably, it is that 30ml is added in every gram of solid powder that deionized water dosage, which is dosage, in the step (3)
Ionized water, water temperature are 75 DEG C, sonication times 20min, ultrasonic power 750w.
Specific embodiment
Embodiment one:
Utilize the method for the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction, comprising the following steps:
(1) autochthonal squama adiposa mycelium is taken, 12h is dried under the conditions of 65 DEG C to constant weight；
(2) the autochthonal squama adiposa mycelium after step (1) drying is crushed at 12000rpm with high speed Universal pulverizer
Then 1min sieves with 100 mesh sieve to obtain bacterium powder；
(3) deionized water is added in autochthonal squama adiposa mycelium powder using ultrasonic wave auxiliary law to be extracted, dosage is
20ml deionized water is added in every gram of solid powder, and water temperature is maintained at 60 DEG C, ultrasonic power 600w, ultrasonic time 10min, weight
It extracts 3 times again and obtains extracting solution；
(4) extracting solution is centrifugated, centrifugal rotational speed 5000rpm, centrifugation time 15min takes supernatant, then will
Supernatant liquor is concentrated under reduced pressure into 2/3rds formation concentrates of original volume at 50 DEG C, then anhydrous second is added in concentrate
Alcohol, the volume of dehydrated alcohol are three times of the volume of the concentrated liquid, are then stood at 4 DEG C for 24 hours, and then centrifuging and taking precipitates again, centrifugation
Revolving speed is 5000rmp, centrifugation time 15min；
(5) gained precipitating successively washed once with acetone, ether, then drying to constant weight at 60 DEG C to get autochthonal
Squama adiposa mycelium polysaccharide.
Embodiment two:
Utilize the method for the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction, comprising the following steps:
(1) autochthonal squama adiposa mycelium is taken, 12h is dried under the conditions of 65 DEG C to constant weight；
(2) the autochthonal squama adiposa mycelium after step (1) drying is crushed at 12000rpm with high speed Universal pulverizer
Then 1min sieves with 100 mesh sieve to obtain bacterium powder；
(3) deionized water is added in autochthonal squama adiposa mycelium powder using ultrasonic wave auxiliary law to be extracted, dosage is
30ml deionized water is added in every gram of solid powder, and water temperature is maintained at 75 DEG C, ultrasonic power 750w, ultrasonic time 20min, weight
It extracts 3 times again and obtains extracting solution；
(4) extracting solution is centrifugated, centrifugal rotational speed 5000rpm, centrifugation time 15min takes supernatant, then will
Supernatant liquor is concentrated under reduced pressure into 2/3rds formation concentrates of original volume at 50 DEG C, then anhydrous second is added in concentrate
Alcohol, the volume of dehydrated alcohol are three times of the volume of the concentrated liquid, are then stood at 4 DEG C for 24 hours, and then centrifuging and taking precipitates again, centrifugation
Revolving speed is 5000rmp, centrifugation time 15min；
(5) gained precipitating successively washed once with acetone, ether, then drying to constant weight at 60 DEG C to get autochthonal
Squama adiposa mycelium polysaccharide.
Embodiment three:
Utilize the method for the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction, comprising the following steps:
(1) autochthonal squama adiposa mycelium is taken, 12h is dried under the conditions of 65 DEG C to constant weight；
(2) the autochthonal squama adiposa mycelium after step (1) drying is crushed at 12000rpm with high speed Universal pulverizer
Then 1min sieves with 100 mesh sieve to obtain bacterium powder；
(3) deionized water is added in autochthonal squama adiposa mycelium powder using ultrasonic wave auxiliary law to be extracted, dosage is
40ml deionized water is added in every gram of solid powder, and water temperature is maintained at 80 DEG C, ultrasonic power 800w, ultrasonic time 30min, weight
It extracts 3 times again and obtains extracting solution；
(4) extracting solution is centrifugated, centrifugal rotational speed 5000rpm, centrifugation time 15min takes supernatant, then will
Supernatant liquor is concentrated under reduced pressure into 2/3rds formation concentrates of original volume at 50 DEG C, then anhydrous second is added in concentrate
Alcohol, the volume of dehydrated alcohol are three times of the volume of the concentrated liquid, are then stood at 4 DEG C for 24 hours, and then centrifuging and taking precipitates again, centrifugation
Revolving speed is 5000rmp, centrifugation time 15min；
(5) gained precipitating successively washed once with acetone, ether, then drying to constant weight at 60 DEG C to get autochthonal
Squama adiposa mycelium polysaccharide.
In above-described embodiment, the instrument of use is as shown in the table:
| Instrument | Model | Company |
| Electronic balance | YP-B1003 | The upper positive Medical Instruments Co., Ltd of sea light |
| High speed Universal pulverizer | LD-T300A | Shanghai Ding Shuai Electrical Appliances Co., Ltd |
| Supersonic wave cleaning machine | SB-3200DTD | NingBo XinZhi Biology Science Co., Ltd |
| Digital display thermostat water bath | HH-4 | Jie Ruier Electrical Appliances Co., Ltd, Jintan City |
| Refrigerator | BCD-221ZE3CK | Hefei Meiling Co. Ltd. |
| Electric drying oven with forced convection | DHG-9240 | Shanghai Yiheng Scientific Instruments Co., Ltd |
| Supercentrifuge | DT5-4D | Shanghai Lu Xiangyi centrifuge Instrument Ltd. |
| Spectrophotometer | 722 | Analyse domain experimental instruments and equipment limited in Shanghai |
The advantages of to further illustrate this method, carries out polysaccharide to this method embodiment two with phend-sulphuric acid now and mentions
Rate is taken to measure:
A, referring to bibliography: Zhang Ying, Wang Hongyuan, Zhou Yuhang wait ultrasonic wave assisted extraction and hot water extraction Schisandra chinensis more
Comparative studies [J] the specialty research of sugar, method described in 2015,3:52-57. is using concentration of glucose as abscissa, absorbance
For ordinate, standard curve is drawn, regression equation is found out.
B, referring to bibliography: the Study on extraction and health-care effect of Han Tianlong, Li Zhiping food fungi fruiting body polysaccharide are visited
Study carefully [J] Jilin Engineering Normal College journal, 2005,21 (03): method described in 14-16. measures product absorbance, and
Go out polysaccharide mass concentration C/ (mg/mL) in product according to regression equation calculation；
C, the autochthonal squama adiosapose polysaccharide mass concentration C obtained by step b, and by product configuration solution volume V/mL and
Autochthonal squama umbrella powder original quality m/mg brings following formula into and calculates:
Polysaccharide extract rate (%)=(CV/m) × 100
It is 9.80% by calculating polysaccharide extract rate.
Claims (2)
1. utilizing the method for the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction, comprising the following steps:
(1) autochthonal squama adiposa mycelium is taken, 12h is dried under the conditions of 65 DEG C to constant weight；
(2) it is right that the autochthonal squama adiposa mycelium high speed Universal pulverizer after drying step (1) crushes 1min at 12000rpm
After sieve with 100 mesh sieve to obtain bacterium powder；
(3) deionized water is added in autochthonal squama adiposa mycelium powder using ultrasonic wave auxiliary law to be extracted, dosage is every gram
20-40ml deionized water is added in solid powder, and water temperature is maintained at 60-80 DEG C, ultrasonic power 600-800w, and ultrasonic time is
10-30min repeats extraction 3 times and obtains extracting solution；
(4) extracting solution is centrifugated, centrifugal rotational speed 5000rpm, centrifugation time 15min takes supernatant, then by upper layer
Clear liquid is concentrated under reduced pressure into 2/3rds formation concentrates of original volume at 50 DEG C, then dehydrated alcohol is added in concentrate, nothing
The volume of water-ethanol is three times of the volume of the concentrated liquid, is then stood at 4 DEG C for 24 hours, and then centrifuging and taking precipitates again, and centrifugal rotational speed is
5000rmp, centrifugation time 15min；
(5) gained precipitating successively washed once with acetone, ether, then drying to constant weight at 60 DEG C to get autochthonal squama umbrella
Mycelium polysaccharides.
2. the method according to claim 1 using the autochthonal squama adiposa mycelium polysaccharide of ultrasonic wave assisted extraction, feature exist
In: it is that 30ml deionized water, water temperature 75 is added in every gram of solid powder that deionized water dosage, which is dosage, in the step (3)
DEG C, sonication times 20min, ultrasonic power 750w.
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| CN115536761A (en) * | 2022-10-26 | 2022-12-30 | 山西农业大学 | Preparation method of edible fungus degradation polysaccharide with anti-inflammatory and antioxidant activities |
| CN116874624A (en) * | 2023-07-03 | 2023-10-13 | 湖南农业大学长沙现代食品创新研究院 | Preparation method and application of gelatinous lepidocroca polysaccharide |
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| CN102505029A (en) * | 2011-11-25 | 2012-06-20 | 南京农业大学 | Method for preparing Dinghu pholiota polysaccharide with anti-tumor activity |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115536761A (en) * | 2022-10-26 | 2022-12-30 | 山西农业大学 | Preparation method of edible fungus degradation polysaccharide with anti-inflammatory and antioxidant activities |
| CN115536761B (en) * | 2022-10-26 | 2023-11-28 | 山西农业大学 | Preparation method of edible fungus degradation polysaccharide with anti-inflammatory and antioxidant activities |
| CN116874624A (en) * | 2023-07-03 | 2023-10-13 | 湖南农业大学长沙现代食品创新研究院 | Preparation method and application of gelatinous lepidocroca polysaccharide |
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