CN109535136B - 2- [4- (2-furyl) ] pyrimidylurea compound and preparation method and application thereof - Google Patents
2- [4- (2-furyl) ] pyrimidylurea compound and preparation method and application thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000001257 hydrogen Substances 0.000 claims abstract description 15
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 15
- 239000000575 pesticide Substances 0.000 claims abstract description 10
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical group [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 claims abstract description 8
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 8
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical group [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims abstract description 4
- 125000004429 atom Chemical group 0.000 claims abstract description 4
- 229910052801 chlorine Inorganic materials 0.000 claims abstract description 4
- 125000001309 chloro group Chemical group Cl* 0.000 claims abstract description 4
- 229910052731 fluorine Inorganic materials 0.000 claims abstract description 4
- 125000001153 fluoro group Chemical group F* 0.000 claims abstract description 4
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 claims abstract description 4
- 229910052740 iodine Inorganic materials 0.000 claims abstract description 4
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims abstract description 4
- 150000001875 compounds Chemical class 0.000 claims description 47
- 238000006243 chemical reaction Methods 0.000 claims description 36
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- 239000000243 solution Substances 0.000 claims description 22
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- KNIKDPRISVIPBU-UHFFFAOYSA-N 1,2-dichloro-4-[isocyano-(4-methylphenyl)sulfonylmethyl]benzene Chemical compound C1=CC(C)=CC=C1S(=O)(=O)C([N+]#[C-])C1=CC=C(Cl)C(Cl)=C1 KNIKDPRISVIPBU-UHFFFAOYSA-N 0.000 claims description 7
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- 230000008569 process Effects 0.000 claims description 4
- KWOLFJPFCHCOCG-UHFFFAOYSA-N Acetophenone Natural products CC(=O)C1=CC=CC=C1 KWOLFJPFCHCOCG-UHFFFAOYSA-N 0.000 claims description 3
- 150000008062 acetophenones Chemical class 0.000 claims description 3
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- 241000406668 Loxodonta cyclotis Species 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 10
- 244000000005 bacterial plant pathogen Species 0.000 abstract description 6
- YLQBMQCUIZJEEH-UHFFFAOYSA-N Furan Chemical group C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 abstract description 5
- 230000002401 inhibitory effect Effects 0.000 abstract description 4
- 230000000607 poisoning effect Effects 0.000 abstract description 3
- 150000001408 amides Chemical class 0.000 abstract description 2
- 150000003230 pyrimidines Chemical class 0.000 abstract description 2
- -1 pyrimidyl urea compound Chemical class 0.000 abstract description 2
- 125000002941 2-furyl group Chemical group O1C([*])=C([H])C([H])=C1[H] 0.000 abstract 2
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- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000005507 spraying Methods 0.000 abstract 1
- IAZDPXIOMUYVGZ-WFGJKAKNSA-N Dimethyl sulfoxide Chemical compound [2H]C([2H])([2H])S(=O)C([2H])([2H])[2H] IAZDPXIOMUYVGZ-WFGJKAKNSA-N 0.000 description 20
- 238000001228 spectrum Methods 0.000 description 14
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- 238000001819 mass spectrum Methods 0.000 description 7
- NTPLXRHDUXRPNE-UHFFFAOYSA-N 4-methoxyacetophenone Chemical compound COC1=CC=C(C(C)=O)C=C1 NTPLXRHDUXRPNE-UHFFFAOYSA-N 0.000 description 6
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- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 5
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/04—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
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- Chemical & Material Sciences (AREA)
- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plural Heterocyclic Compounds (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a 2- [4- (2-furyl)]The pyrimidyl urea compound, the preparation method and the application thereof have the structural general formula:
in the general formula R1Is fluorine atom, chlorine atom, bromine atom, iodine atom, methyl group, methoxy group, R2Hydrogen atom, bromine atom, methyl group, hydroxymethyl group. Provided by the present applicationCompounds 2- [4- (2-furyl) are synthesized by introducing furan ring and amide into the molecular structure of pyrimidine]Pyrimidinylurea compounds. The pesticide spraying agent has simple structure, has better poisoning effect on the stored pests, has better inhibitory activity on plant pathogenic bacteria, and lays a good foundation for the creation of new pesticides.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a 2- [4- (2-furyl) ] pyrimidylurea compound and a preparation method and application thereof.
Background
The amide compounds have wide biological activity, including sterilization, antioxidation, plant growth regulation and the like. Therefore, extensive and intensive research on the compounds has been conducted over the past decades, and many novel and highly effective compounds have been discovered, and there have been continuously reported varieties which are novel in structure, unique in mode of action, excellent in performance, highly effective against harmful organisms, safe against non-target organisms, easily degradable in the environment, and safe in the human health and ecological environment as degradation products.
The pyrimidine is a very important heterocyclic compound and is widely applied to the fields of medicines, pesticides, daily chemicals and the like. A large number of researches show that the compounds have good biological activity, such as insecticidal activity, bactericidal activity, acaricidal activity, weeding activity, plant growth regulation activity, antiviral activity, anticancer activity, antioxidant activity, anti-inflammatory activity and the like. Because the pyrimidine compounds have the advantages of high efficiency, low toxicity to human and livestock, unique action mode, difficult generation of drug resistance of pests and the like, the molecular structure design, synthesis and biological activity research are very active fields in the heterocyclic compound research, and the compounds with novel and simple structure and good biological activity are continuously generated.
It is known that furan ring is an electron-rich system, which easily forms intermolecular hydrogen bonds with various biological enzymes, and thus some furan ring-containing compounds, both natural and synthetic, have a broad spectrum of biological activities, such as bacteriostasis, antivirus, antitumor, insecticidal, herbicidal, etc. Meanwhile, the compounds generally have the characteristics of high efficiency, low toxicity, safety to non-target organisms, easiness in degradation in the environment, difficulty in generation of resistance by pests and the like, so that the compounds containing furan rings play more and more important roles in the research and development processes of pesticides.
To date, the use of 2- [4- (2-furyl) ] pyrimidinylurea compounds as agricultural insecticides and fungicides has not been reported.
Disclosure of Invention
The invention aims to provide a novel compound used as an agricultural insecticide and a bactericide and a preparation method thereof.
A2- [4- (2-furyl) ] pyrimidyl urea compound has the following structural general formula:
r in the general formula (I)1Is fluorine atom, chlorine atom, bromine atom, iodine atom, methyl group, methoxy group, R2Hydrogen atom, bromine atom, methyl group, hydroxymethyl group.
A preparation method of 2- [4- (2-furyl) ] pyrimidyl urea compounds comprises the following steps: dissolving substituted acetophenone in anhydrous ethanol, adding 10% NaOH solution, stirring in ice bath, slowly dropping the mixture of furfural and anhydrous ethanol or the mixture of 5-methylfurfural and anhydrous ethanol into the above mixed solution with a constant pressure dropping funnel, reacting at 0-5 deg.C, and checking whether the reaction is completed with thin-layer silica gel plate (TLC). After completion of the reaction, 13X molecular sieves were added to the reaction mixture, and a mixed solution of guanylurea sulfate and absolute ethanol was slowly dropped into the mixture using a constant pressure dropping funnel, followed by reaction at 40 to 50 ℃ and completion of the reaction was checked by TLC. And after the reaction is finished, filtering, adding a large amount of ice water into the filtrate, adjusting the pH value to be neutral by using a 10% hydrochloric acid solution, separating out a precipitate, filtering, washing, and recrystallizing by using absolute ethyl alcohol to obtain the target compound.
Further, according to the method, the substituted acetophenone is one of 4-chloroacetophenone, 4-methoxyacetophenone and 4-methylacetophenone.
Further, in the method described above, the 13X molecular sieve is 40-60 mesh.
An application of 2- [4- (2-furyl) ] pyrimidyl urea compound as agricultural insecticide or bactericide.
Further, as the above-mentioned application, the pesticide can kill one or more of corn elephant, corn bagel and corn beetle.
Further, the bactericide can inhibit one or more of bacterial brown spot of corn, bacterial black spot of rape, citrus canker, potato black shank, onion bulb rot and melon bacterial fruit blotch.
Has the advantages that:
the invention introduces furan ring and amide into the molecular structure of pyrimidine to synthesize some 2- [4- (2-furyl) ] pyrimidyl urea compounds. They have simple structure and excellent activity, and will lay a good foundation for the creation of new pesticides.
The compound of the general formula (I) has simple synthesis process, and adopts a one-pot method, namely, the intermediate is not separated out according to the traditional method and then subjected to the next reaction, but the next reaction is directly performed, so that the operation steps are reduced, the reaction efficiency is improved, and the energy conservation and consumption reduction are facilitated. The compound of the general formula (I) has better control effect on storage pests and plant pathogenic bacteria, and is not reported in the currently known pesticides and bactericides.
Drawings
FIG. 1 is a hydrogen spectrum of the compound prepared in example 1;
FIG. 2 is a carbon spectrum of the compound prepared in example 1;
FIG. 3 is a high resolution mass spectrum of the compound prepared in example 1;
FIG. 4 is a hydrogen spectrum of the compound prepared in example 2;
FIG. 5 is a carbon spectrum of the compound prepared in example 2;
FIG. 6 is a high resolution mass spectrum of the compound prepared in example 2;
FIG. 7 is a hydrogen spectrum of the compound prepared in example 3;
FIG. 8 is a carbon spectrum of the compound prepared in example 3;
FIG. 9 is a high resolution mass spectrum of the compound prepared in example 3;
FIG. 10 is a hydrogen spectrum of the compound prepared in example 4;
FIG. 11 is a carbon spectrum of the compound prepared in example 4;
FIG. 12 is a high resolution mass spectrum of the compound prepared in example 4;
FIG. 13 is a hydrogen spectrum of the compound prepared in example 5;
FIG. 14 is a carbon spectrum of the compound prepared in example 5;
FIG. 15 is a high resolution mass spectrum of the compound prepared in example 5.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention are described below clearly and completely, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a compound with insecticidal and bacteriostatic activity, which has the following structural general formula:
r in the general formula (I)1Is fluorine atom, chlorine atom, bromine atom, iodine atom, methyl group, methoxy group, R2Hydrogen atom, bromine atom, methyl group, hydroxymethyl group.
The main reaction equation in the preparation of the compound of formula (I) is:
in the formula R1、R2The same as above.
The compound of the general formula (I) has simple synthesis process, and adopts a one-pot method, namely, the intermediate is not separated out according to the traditional method and then subjected to the next reaction, but the next reaction is directly performed, so that the operation steps are reduced, the reaction efficiency is improved, and the energy conservation and consumption reduction are facilitated. The compound of the general formula (I) has better control effect on storage pests and plant pathogenic bacteria, and is not reported in the currently known pesticides and bactericides.
Example 1:
compound (I)
Preparation of
0.02mol of 4-methoxyacetophenone was dissolved in 20mL of anhydrous ethanol, and 15mL of a 10% NaOH solution was added thereto. Under the stirring of ice bath, a mixed solution of 0.02mol of furfural and 20mL of absolute ethyl alcohol is slowly dropped into the mixed solution by using a constant pressure dropping funnel, the reaction is carried out at 0-5 ℃, and whether the reaction is finished or not is checked by using a thin-layer silica gel plate (TLC). After completion of the reaction, 2 g of a 13X molecular sieve (40-60 mesh) was added to the reaction mixture, and a mixed solution of 0.02mol of guanylurea sulfate and 20mL of anhydrous ethanol was slowly dropped into the mixture using a constant pressure dropping funnel, reacted at 40-50 ℃ and checked for completion by TLC. After the reaction is finished, filtering, adding a large amount of ice water into the filtrate, adjusting the pH value to be neutral by using a 10% hydrochloric acid solution, separating out a precipitate, filtering, washing, and recrystallizing by using absolute ethyl alcohol to obtain a light yellow powdery solid product, wherein the yield is 81%. Spectral data for the product are as follows:
1H NMR(400MHz,DMSO-d6)δ(ppm):9.56(1H,s),8.71(1H,s),8.19(2H,d,J＝8.8Hz),8.02(1H,d,J＝0.8Hz),7.84(1H,s),7.52(1H,d,J＝3.6Hz),7.16(1H,s),7.12(2H,d,J＝8.8Hz),6.78(1H,dd,J1＝3.6Hz,J2＝3.6Hz),3.86(3H,s)；13C NMR(100MHz,DMSO-d6)δ(ppm):164.82,162.45,158.96,155.81,155.32,151.29,146.73,129.31,128.62,114.79,113.94,113.29,103.39,55.91；HRMS(ESI)m/z:Calcd for C16H14N4O3[M+Na]+:333.0959,Found:333.0939.
the hydrogen spectrogram, carbon spectrogram and high-resolution mass spectrogram of the product are shown in figures 1-3.
Example 2:
compound (I)
Preparation of
0.02mol of 4-chloroacetophenone was dissolved in 20mL of anhydrous ethanol, and 15mL of 10% NaOH solution was added thereto. Under the stirring of ice bath, a mixed solution of 0.02mol of 5-methylfurfural and 20mL of anhydrous ethanol is slowly dropped into the mixed solution by using a constant pressure dropping funnel, the reaction is carried out at 0-5 ℃, and whether the reaction is finished or not is checked by using a thin-layer silica gel plate (TLC). After completion of the reaction, 2 g of a 13X molecular sieve (40-60 mesh) was added to the reaction mixture, and a mixed solution of 0.02mol of guanylurea sulfate and 20mL of anhydrous ethanol was slowly dropped into the mixture using a constant pressure dropping funnel, reacted at 40-50 ℃ and checked for completion by TLC. After the reaction is finished, filtering, adding a large amount of ice water into the filtrate, adjusting the pH value to be neutral by using a 10% hydrochloric acid solution, separating out a precipitate, filtering, washing, and recrystallizing by using absolute ethyl alcohol to obtain a white needle-shaped crystal product, wherein the yield is 86%. Spectral data for the product are as follows:
1H NMR(400MHz,DMSO-d6)δ(ppm):9.57(1H,s),8.72(1H,s),8.20(2H,d,J＝8.8Hz),8.03(1H,s),7.85(1H,s),7.53(1H,s),7.13(2H,d,J＝8.8Hz),6.80(1H,s),2.43(3H,s)；13C NMR(100MHz,DMSO-d6)δ(ppm):164.26,162.04,155.27,150.71,138.22,133.44,132.16,131.36,128.91,127.29,114.08,109.38,100.05,14.09；HRMS(ESI)m/z:Calcd for C16H13N4O2Cl[M+H]+:322.0805,Found:322.0836.
the hydrogen spectrogram, carbon spectrogram and high-resolution mass spectrogram of the product are shown in FIGS. 4-6.
Example 3:
compound (I)
Preparation of
0.02mol of 4-methylacetophenone was dissolved in 20mL of anhydrous ethanol, and 15mL of 10% NaOH solution was added thereto. Under the stirring of ice bath, a mixed solution of 0.02mol of 5-methylfurfural and 20mL of anhydrous ethanol is slowly dropped into the mixed solution by using a constant pressure dropping funnel, the reaction is carried out at 0-5 ℃, and whether the reaction is finished or not is checked by using a thin-layer silica gel plate (TLC). After completion of the reaction, 2 g of a 13X molecular sieve (40-60 mesh) was added to the reaction mixture, and a mixed solution of 0.02mol of guanylurea sulfate and 20mL of anhydrous ethanol was slowly dropped into the mixture using a constant pressure dropping funnel, reacted at 40-50 ℃ and checked for completion by TLC. After the reaction is finished, filtering, adding a large amount of ice water into the filtrate, adjusting the pH value to be neutral by using a 10% hydrochloric acid solution, separating out a precipitate, filtering, washing, and recrystallizing by using absolute ethyl alcohol to obtain a white solid product, wherein the yield is 91%. Spectral data for the product are as follows:
1H NMR(400MHz,DMSO-d6)δ(ppm):9.57(1H,s),8.72(1H,s),8.20(2H,d,J＝8.8Hz),8.04(1H,s),7.85(1H,s),7.53(1H,d,J＝3.6Hz),7.13(2H,d,J＝8.8Hz),6.80(1H,dd,J1＝3.6Hz,J2＝3.6Hz),2.44(3H,s),2.41(3H,s)；13C NMR(100MHz,DMSO-d6)δ(ppm):165.82,163.45,159.96,156.81,156.32,152.29,147.73,130.31,129.62,115.79,114.94,114.29,104.39,21.23,14.01；HRMS(ESI)m/z:Calcd for C17H16N4O2[M+H]+:309.1351,Found:309.1343.
the hydrogen spectrogram, carbon spectrogram and high-resolution mass spectrogram of the product are shown in FIGS. 7-9.
Example 4:
compound (I)
Preparation of
0.02mol of 4-methoxyacetophenone was dissolved in 20mL of anhydrous ethanol, and 15mL of a 10% NaOH solution was added thereto. Under the stirring of ice bath, a mixed solution of 0.02mol of 5-methylfurfural and 20mL of anhydrous ethanol is slowly dropped into the mixed solution by using a constant pressure dropping funnel, the reaction is carried out at 0-5 ℃, and whether the reaction is finished or not is checked by using a thin-layer silica gel plate (TLC). After completion of the reaction, 2 g of a 13X molecular sieve (40-60 mesh) was added to the reaction mixture, and a mixed solution of 0.02mol of guanylurea sulfate and 20mL of anhydrous ethanol was slowly dropped into the mixture using a constant pressure dropping funnel, reacted at 40-50 ℃ and checked for completion by TLC. After the reaction is finished, filtering, adding a large amount of ice water into the filtrate, adjusting the pH value to be neutral by using a 10% hydrochloric acid solution, separating out a precipitate, filtering, washing, and recrystallizing by using absolute ethyl alcohol to obtain a gray solid product, wherein the yield is 83%. Spectral data for the product are as follows:
1H NMR(400MHz,DMSO-d6)δ(ppm):9.56(1H,s),8.71(1H,s),8.19(2H,d,J＝8.8Hz),8.03(1H,s),7.84(1H,s),7.52(1H,d,J＝3.6Hz),7.16(1H,s),7.12(2H,d,J＝8.8Hz),3.87(3H,s),2.40(3H,s)；13C NMR(100MHz,DMSO-d6)δ(ppm):162.76,161.33,155.45,154.90,154.78,153.29,142.83,131.40,128.27,114.37,107.86,106.35,104.95,56.30,14.76；HRMS(ESI)m/z:Calcd for C17H16N4O3[M+Na]+:347.1117,Found:347.1106.
the hydrogen spectrum, carbon spectrum and high resolution mass spectrum of the product are shown in FIGS. 10-12.
Example 5:
compound (I)
Preparation of
0.02mol of 4-chloroacetophenone was dissolved in 20mL of anhydrous ethanol, and 15mL of 10% NaOH solution was added thereto. Under the stirring of ice bath, a mixed solution of 0.02mol of furfural and 20mL of absolute ethyl alcohol is slowly dropped into the mixed solution by using a constant pressure dropping funnel, the reaction is carried out at 0-5 ℃, and whether the reaction is finished or not is checked by using a thin-layer silica gel plate (TLC). After completion of the reaction, 2 g of a 13X molecular sieve (40-60 mesh) was added to the reaction mixture, and a mixed solution of 0.02mol of guanylurea sulfate and 20mL of anhydrous ethanol was slowly dropped into the mixture using a constant pressure dropping funnel, reacted at 40-50 ℃ and checked for completion by TLC. After the reaction is finished, filtering, adding a large amount of ice water into the filtrate, adjusting the pH value to be neutral by using a 10% hydrochloric acid solution, separating out a precipitate, filtering, washing, and recrystallizing by using absolute ethyl alcohol to obtain a light yellow powdery solid product, wherein the yield is 90%. Spectral data for the product are as follows:
1H NMR(400MHz,DMSO-d6)δ(ppm):9.64(1H,s),8.64(1H,s),8.28(2H,dd,J1＝8.4Hz,J2＝8.4Hz),8.03(1H,s),7.90(1H,s),7.55(1H,d,J＝3.2Hz),7.41(2H,t,J＝8.8Hz),7.18(1H,s),6.79(1H,dd,J1＝3.2Hz,J2＝3.2Hz)；13C NMR(100MHz,DMSO-d6)δ(ppm):164.19,159.00,156.13,155.24,151.12,146.92,130.15,130.06,116.50,116.28,114.33,113.35,104.14；HRMS(ESI)m/z:Calcd for C15H11N4O2Cl[M+Na]+:337.0464,Found:337.0458.
the hydrogen spectrum, carbon spectrum and high resolution mass spectrum of the product are shown in FIGS. 13-15.
Example 6: determination of insecticidal Activity of Compounds of the present invention
(1) Test pest
Adult corn weevils, adult corn clothes and adult tribolium castaneum, which are all sensitive strains bred indoors for years.
(2) Measurement method
Adopting a feed mixing method: mixing the compound to be tested and the wheat feed uniformly according to a certain dosage. Weighing 100 g of the drug-mixed feed into 500mL wide-mouth bottles, putting 30 heads of tested pests into each bottle, wrapping the bottle mouth with white cloth, placing the bottles in a pest feeding room with the temperature of 28-30 ℃ and the relative humidity of 70-80% for continuous feeding, and taking the feed without the drug as a blank control. Mortality was recorded after 14 days, each experiment was repeated 3 times, and corrected mortality was calculated using the following formula:
(3) results of the experiment
The insecticidal results of the compounds of the present invention are shown in table 1.
TABLE 1 poisoning Activity of Compounds of the invention against storage pests
a: average of three replicates.
From Table 1 above, it is clear that the compounds of the present invention have a good poisoning activity against these pests.
Example 7: determination of the bacteriostatic Activity of Compounds of the invention
(1) Test for plant pathogenic bacteria
Bacterial brown spot of corn, bacterial black spot of rape, canker of citrus, black shank of potato, bulb rot of onion, and bacterial fruit blotch of melon.
(2) Measurement method
(a) Activation of strains: the bacterial strain to be tested is inoculated on a beef extract peptone solid medium slant and cultured overnight at 37 ℃.
(b) Preparation of bacterial suspension: in a container with 100mInoculating a ring of activated test strain in conical flask of L beef extract peptone liquid culture medium, culturing at 37 deg.C for 18 hr to obtain initial bacterial suspension, and diluting with sterile normal saline to appropriate concentration (10)6～107CFU/mL) of the suspension.
(c) Determination of Minimum Inhibitory Concentration (MIC): dissolving the test compound in dimethyl sulfoxide, diluting with sterile normal saline containing 0.1% Tween-80 by two-fold dilution method to obtain solutions with different concentrations, and mixing. 1mL of the diluted sample solution was added to 19mL of a sterilized medium, and mixed well to prepare a plate. After the culture medium has solidified, the above-mentioned concentration of 10% is added by coating method6And culturing 200 mu L of CFU/mL bacterial suspension at 37 ℃ for 16-18 h, observing the growth condition of bacteria, taking the concentration of completely sterile growth as the MIC value of the test sample solution, and taking the corresponding solution without the test compound as a blank control.
(3) Results of the experiment
The bacteriostatic activity of the compounds of the invention is shown in table 2.
TABLE 2 inhibitory Activity of the Compounds of the present invention against plant pathogenic bacteria
From the above table 2, it can be seen that the compounds of the present invention have a good inhibitory effect on these plant pathogenic bacteria.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (4)
1. A2- [4- (2-furyl) ] pyrimidyl urea compound is characterized by comprising the following structural general formula:
r in the general formula (I)1Is fluorine atom, chlorine atom, bromine atom, iodine atom, methyl group, methoxy group, R2Hydrogen atom, bromine atom, methyl group, hydroxymethyl group.
2. A process for the preparation of 2- [4- (2-furyl) ] pyrimidinylurea compounds according to claim 1, characterized in that: dissolving substituted acetophenone in anhydrous ethanol, adding 10% NaOH solution, slowly dropping a mixed solution of furfural and anhydrous ethanol or a mixed solution of 5-methylfurfural and anhydrous ethanol into the mixed solution by using a constant-pressure dropping funnel under ice-bath stirring, reacting at 0-5 ℃, and checking whether the reaction is finished by using a thin-layer silica gel plate; after the reaction is finished, adding a 13X molecular sieve into the reaction mixture, slowly dropping a mixed solution of guanylurea sulfate and absolute ethyl alcohol into the mixture by using a constant-pressure dropping funnel, reacting at 40-50 ℃, and detecting whether the reaction is finished by TLC; and after the reaction is finished, filtering, adding a large amount of ice water into the filtrate, adjusting the pH value to be neutral by using a 10% hydrochloric acid solution, separating out a precipitate, filtering, washing, and recrystallizing by using absolute ethyl alcohol to obtain the target compound.
3. The method of claim 2, wherein the 13X molecular sieve is 40-60 mesh.
4. The use of 2- [4- (2-furyl) ] pyrimidinylurea compounds according to claim 1 as agricultural pesticides which kill one or more of elephant corn, clothes of corn and corn beetle.
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