CN109529117A - A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket - Google Patents
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket Download PDFInfo
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- CN109529117A CN109529117A CN201811654044.0A CN201811654044A CN109529117A CN 109529117 A CN109529117 A CN 109529117A CN 201811654044 A CN201811654044 A CN 201811654044A CN 109529117 A CN109529117 A CN 109529117A
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- fibroin albumen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/04—Dry spinning methods
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
- D01F4/02—Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
- A61L2300/254—Enzymes, proenzymes
Abstract
The technical program discloses a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket, comprising the following steps: (1) dissolves boiled silk and obtain silk fibroin water solution；(2) concentration silk fibroin water solution obtains the fibroin albumen spinning solution that concentration is 8~45%；(3) activated protein solution is added, activity fibroin albumen spinning solution is made；(4) it is pulling motion in the pressure-air injection stream that temperature is 0~37 DEG C, carries out molten spray spinning, and receive spinning with fiber receiver；And ethyl alcohol spraying device is equipped on the fiber receiver, the ethyl alcohol spraying device post-processes spinning, and fibrous framework is made；(5) living solution containing seed cell or Porcine HGF is printed in step (4) and is made in fibrous framework, it is final that active fibroin albumen bioengineered tissue bracket is made.Active fibroin albumen bioengineered tissue bracket using preparation method preparation has high porosity and good hole link, can provide good microenvironment for the growth of cell.
Description
Technical field
The technical program belongs to biomedical material technology, in particular to a kind of active fibroin albumen biological tissue work
The molten spray Preparation Method of engineering support.
Background technique
Fibroin albumen is made of 18 kinds of amino acid such as Gly, Ala, Ser, is had both sexes charge, is a kind of natural structure egg
It is white.It is obtained by silk through sericin removal, is biocompatibility in nature, the good natural polymer of biodegradable.
Structure and function of the fibroin albumen bioengineered tissue bracket because that can imitate extracellular matrix to a certain extent be
Cell provides the microenvironment of ideal growth, proliferation and differentiation, in recent years, be widely used in include cartilage, bone,
The every field of the Tissue Engineering Studies such as blood vessel, skin.Electrostatic spinning technique is the unique of current active fibroin fiber film
Mechanics when technology of preparing, the mechanical performance of fibroin albumen bioengineered tissue bracket can satisfy different tissues engineer application is strong
Degree demand.However, the mechanical property for just spinning obtained fibroin albumen bioengineered tissue bracket is poor, it is necessary to after certain
After treatment process processing, tissue engineering bracket can be only achieved using required mechanical strength requirement.The presence of aftertreatment technology is just
Increase the production cost of fibroin albumen bioengineered tissue bracket.
Electrostatic spinning technique, which prepares fibroin albumen bioengineered tissue bracket, also has disadvantage: (1) high voltage is needed,
It is defective in terms of operational safety, it is not easy to large-scale application in the industrial production, and high voltage can reduce fibroin albumen
The activity of middle active constituent；(2) during the electrostatic spinning that speed is exceedingly fast, single electrostatic stretch power effect can not promote silk
Fibroin molecule is changed into beta sheet structure by random coil/α-helixstructure under aqueous solution state, so that fibroin albumen is difficult
Crystalline rearrangement occurs, cause the fibre crystallinity being collected into low, poor mechanical property.
Summary of the invention
The technical program provides a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket, the preparation
The animal spinning process of method energy natural imitation circle, and lead to the active fibroin albumen biological tissue work of the preparation method preparation
The mechanical property of engineering support is good.
To achieve the above object, following technological means that the technical program uses.
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket, comprising the following steps:
(1) it using boiled silk as raw material, under 40 DEG C of water baths, is dissolved boiled silk 2~12 hours, is obtained using solvent
To silk fibroin water solution；
(2) silk fibroin water solution made from step (1) be centrifuged, filtered, being dialysed and concentration after, obtain dense
The fibroin albumen spinning solution that degree is 12~45%；
(3) activated protein solution is added in silk fibroin water solution obtained in step (2), activity fibroin albumen is made
Spinning solution, the activated protein concentration in added activated protein solution are 5~15%；
(4) by activity fibroin albumen spinning solution made from step (3), the pressure-air injection stream for being 0~37 DEG C in temperature
For pulling motion, molten spray spinning is carried out, and receives spinning with fiber receiver；And ethyl alcohol is equipped on the fiber receiver
Spraying device, the ethyl alcohol spraying device post-process spinning, realize fibroin albumen conformation in spinning fibre forming process
Transformation, be made fibrous framework；
(5) cell printing device is equipped with after fiber receiver, by the work containing seed cell or Porcine HGF
Property solution print to and be made in step (4) in tunica fibrosa, it is final that active fibroin albumen bioengineered tissue bracket is made.
Further, the activated protein in the activated protein solution is immunoglobulin, lactoferrin, metallothionein
Any one of white, superoxide dismutase, soybean protein, lysozyme.
Further, the solvent of the dissolution boiled silk is LiBr aqueous solution or ethyl alcohol/CaCl aqueous solution.
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket is further comprising the steps of: to step
(1) silk fibroin protein solution made from is added pH adjusting agent and carries out pH value adjusting, and the pH adjusting agent of addition is metal ion
Or inorganic nanometer powder.
Further, the metal ion is one of calcium ion, silver ion.
Further, the inorganic nanometer powder is one of hydroxyapatite, tricalcium phosphate.
Further, the ethyl alcohol spray velocity in the ethyl alcohol spraying device is 100~1000mL/min, and ethyl alcohol used
Concentration is 50~100%.
Further, the fiber receiver is that fiber receives plate or fiber receives roller；When the fiber receiver
When receiving plate using fiber, active fibroin fiber film is obtained；When the fiber receiver connects using fiber
When receiving roller, the active fibroin albumen continuous fiber film of simple tension and one direction ordered arrangement is obtained.
Further, pressure-air injection stream described in step (2) is by dry compressed air, relative humidity
It is 10%~50%.
Further, the injection pressure in pressure-air injection stream used is 10MPa~30MPa.
Having the beneficial effect that in the technical program for the technical program dissolves boiled silk using under 40 DEG C of water baths
2~12 hours, dissolving with this condition to boiled silk can be while guaranteeing that boiled silk can sufficiently dissolve, also
It can guarantee that fibroin albumen therein is not decomposed, guarantee the integrality of fibroin albumen structure；And add in silk fibroin protein solution
Add activated protein solution, the addition of reactive protein, which can enhance, improves the active fibroin albumen bioengineered tissue branch
The cell compatibility of frame, and be conducive to the adherency of seed cell, proliferation, and can the effectively special gene table of active cell
It reaches, maintains the phenotypic expression of cell；The active fibroin albumen biology group prepared using preparation method described in the technical program
The diameter of fibroin fiber in weaver's engineering support is micron and sub-micron rank, it might even be possible to reach Nano grade, it can be with
Farthest imitate the structure of human body cell epimatrix；Voltage is not needed in preparation method described in the technical program, and
And the preparation method does not use organic solvent, and it is operational and highly-safe and environmentally friendly, it can be in the industrial production
Large-scale application；In the technical program above spinning receiver be provided with alcohol vapour spraying device to fibroin fiber into
Row ethyl alcohol spraying treatment, can promote the further transformation of SF structure in fibroin fiber in this way, to improve its mechanical property
Energy；Preparation method described in the technical program can be by adjusting machined parameters, so that the active fibroin albumen biology of preparation
Porosity in tissue engineering bracket meets the needs of different cells growth；The active fibroin egg prepared using the preparation method
White bioengineered tissue bracket has high porosity and good hole link, and also has big specific surface area, energy
Good microenvironment is provided for the growth of cell, is conducive to the adherency, differentiation and proliferation of cell.
Specific embodiment
Technical solution of the present invention is further illustrated combined with specific embodiments below.It should be understood that these embodiments are only used for
Illustrate the present invention rather than limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, this
Field technical staff can make various changes or modifications the present invention, and such equivalent forms equally fall within right appended by the application and want
Seek book limited range.
Embodiment 1
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket, comprising the following steps: (1) by silk cocoon
With aqueous sodium carbonate degumming, boiled silk is obtained, boiled silk is dissolved by solvent of the lithium bromide water solution of 9mol/L, 40
It is dissolved boiled silk 2 hours under DEG C water bath, obtains silk fibroin water solution；(2) after centrifugation, filtering, dialysis and concentration,
Obtain the fibroin albumen spinning solution that concentration is 12wt%；(3) concentration is added into fibroin albumen spinning solution made from step (2) is
Activity fibroin albumen spinning solution is made in 5% immunoglobulin solution；(4) with 10 DEG C of temperature, the high pressure that relative humidity is 10%
Air jet stream is pulling motion, and the injection pressure of pressure-air injection stream used is 30MPa, to active fibroin albumen spinning solution
It is stretched, ethyl alcohol spray velocity is 100mL/min above fiber receiver, and concentration of alcohol is 80%, and fiber used receives
Device is that fiber receives plate, and fibroin fiber film is made；(5) cell is carried out to fibroin fiber film made from step (4)
Printing, wherein cell printing liquid be containing concentration be 2% fibroblast growth factor and concentration be 30% skin at fibre
The bovine serum albumin aqueous solution for tieing up cell finally obtains active fibroin fiber film, and average fibre diameter is 1 μm.
Embodiment 2
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket, comprising the following steps: (1) by silk cocoon
With aqueous sodium carbonate degumming, boiled silk is obtained, boiled silk is dissolved by solvent of the lithium bromide water solution of 9mol/L, 40
It is dissolved boiled silk 2 hours under DEG C water bath, obtains silk fibroin water solution；(2) after centrifugation, filtering, dialysis and concentration,
Obtain the fibroin albumen spinning solution that concentration is 12wt%；(3) concentration is added into fibroin albumen spinning solution made from step (2) is
Activity fibroin albumen spinning solution is made in 15% immunoglobulin solution；(4) with 10 DEG C of temperature, the height that relative humidity is 10%
Pressure air jet stream is pulling motion, and the injection pressure of pressure-air injection stream used is 20MPa, to active fibroin albumen spinning
Liquid is stretched, and ethyl alcohol spray velocity is 100mL/min above fiber receiver, and concentration of alcohol is 50%, and the fiber connects
Receiving device is that fiber receives plate, and fibroin fiber film is made；(5) fibroin fiber film made from step (4) is carried out thin
Born of the same parents printing, wherein cell printing liquid be containing concentration be 10% vascular endothelial growth factor and concentration be 30% umbilical vein in
The bovine serum albumin aqueous solution of chrotoplast；Active fibroin fiber film is finally obtained, and average fibre diameter is 2 μm.
Embodiment 3
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket, comprising the following steps: (1) by silk cocoon
With aqueous sodium carbonate degumming, boiled silk is obtained, boiled silk is dissolved by solvent of the lithium bromide water solution of 9mol/L, 40
It is dissolved boiled silk 12 hours under DEG C water bath, obtains silk fibroin water solution；(2) through centrifugation, filtering, dialyse and be concentrated into
After 20wt%, adding CaCl calcium ion concentration into silk fibroin water solution is 0.3mol/L, continues to be concentrated into fibroin albumen dense
Degree is 45wt%；(3) immunoglobulin solution that concentration is 10% is added into fibroin albumen spinning solution made from step (2),
Activity fibroin albumen spinning solution is made；(4) with 37 DEG C of temperature, the pressure-air injection stream that relative humidity is 50% is to stretch to move
The injection pressure of power, pressure-air injection stream used is 25MPa, is stretched to active fibroin albumen spinning solution, and fiber receives
Ethyl alcohol spray velocity is 500mL/min above device, and concentration of alcohol is 100%, and fiber receiver used is that fiber receives plate,
Fibroin fiber film is made；(5) cell printing is carried out to fibroin fiber film made from step (4), wherein cell printing
Liquid be containing concentration be 4% vascular endothelial growth factor and concentration be 30% huve cell bovine serum albumin water
Solution；Active fibroin fiber film is finally obtained, and average fibre diameter is 10 μm.
Embodiment 4
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket, comprising the following steps: (1) by silk cocoon
With aqueous sodium carbonate degumming, boiled silk is obtained, dissolves degumming silkworm by solvent of calcium chloride/ethanol water of 7mol/L
Silk, dissolves boiled silk 6 hours under 40 DEG C of water baths, obtains silk fibroin water solution；(2) through centrifugation, filtering, dialysis and
Concentration obtains the spinning solution that fibroin albumen concentration is 12wt%；(3) modified nanometer hydroxyapatite is added to fibroin egg
In white water solution, after mixing evenly, it is concentrated into 30wt%；(4) concentration is added into fibroin albumen spinning solution made from step (2)
For 10% immunoglobulin solution, activity fibroin albumen spinning solution is made；(5) with 37 DEG C of temperature, relative humidity is 50%
Pressure-air injection stream is pulling motion, and the injection pressure of pressure-air injection stream used is 15MPa, is spun to active fibroin albumen
Silk liquid is stretched, and ethyl alcohol spray velocity is 500mL/min above fiber receiver, and concentration of alcohol is 80%, fiber used
Receiver is that fiber receives plate, and fibroin fiber film is made；(6) fibroin fiber film made from step (5) is carried out
Cell printing, wherein cell printing liquid is the marrow for being 20% containing transforming grouth factor beta 2 and concentration for being 4% containing concentration
The stock morphogenetic protein white water solution of mescenchymal stem cell finally obtains active fibroin fiber film, and average fibre diameter
It is 5 μm.
Embodiment 5
A kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket, comprising the following steps: (1) by silk cocoon
With aqueous sodium carbonate degumming, boiled silk is obtained, dissolves degumming silkworm by solvent of calcium chloride/ethanol water of 7mol/L
Silk, dissolves boiled silk 12 hours under 40 DEG C of water baths, obtains silk fibroin water solution；(2) through centrifugation, filtering, dialysis
And concentration, obtain the spinning solution that fibroin albumen concentration is 20wt%；(3) add into fibroin albumen spinning solution made from step (2)
Enter the immunoglobulin solution that concentration is 15%, activity fibroin albumen spinning solution is made；(4) with 10 DEG C of temperature, relative humidity is
10% pressure-air injection stream is pulling motion, and the injection pressure of pressure-air injection stream used is 30MPa, to active fibroin
Albumen spinning solution is stretched, and ethyl alcohol spray velocity is 500mL/min above fiber receiver, and concentration of alcohol is 80%, system
Obtain fibroin fiber film；(5) cell printing is carried out to fibroin fiber film made from step (4), wherein cell printing liquid
For the transforming growth factor β 3 and concentration that are 5% containing concentration be 25% articular chondrocytes bone morphogenetic protein it is water-soluble
Liquid；Active fibroin fiber film is finally obtained, and average fibre diameter is 3 μm.
The better embodiment of the technical program is illustrated above, but the technical program be not limited to it is described
Embodiment, those skilled in the art can also make various equivalent changes under the premise of without prejudice to the technical program spirit
Type or replacement, these equivalent variation or replacement are all included in the scope defined by the claims of the present application.
Claims (10)
1. a kind of molten spray Preparation Method of activity fibroin albumen bioengineered tissue bracket, which comprises the following steps:
(1) it using boiled silk as raw material, under 40 DEG C of water baths, is dissolved boiled silk 2~12 hours using solvent, obtains silk
Fibroin aqueous solution；
(2) silk fibroin water solution made from step (1) be centrifuged, filtered, being dialysed and concentration after, obtaining concentration is
8~45% fibroin albumen spinning solution；
(3) activated protein solution is added in silk fibroin water solution obtained in step (2), activity fibroin albumen spinning is made
Liquid, the activated protein concentration in added activated protein solution are 5~15%；
It (4) is drawing in the pressure-air injection stream that temperature is 0~37 DEG C by activity fibroin albumen spinning solution made from step (3)
Power is stretched, carries out molten spray spinning, and receive spinning with fiber receiver；And it is spraying equipped with ethyl alcohol on the fiber receiver
Device, the ethyl alcohol spraying device post-process spinning, realize turn of fibroin albumen conformation in spinning fibre forming process
Become, fibrous framework is made；
(5) cell printing device is equipped with after fiber receiver, the activity containing seed cell or Porcine HGF is molten
Liquid is printed in step (4) and is made in fibrous framework, final that active fibroin albumen bioengineered tissue bracket is made.
2. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 1, special
Sign is that the activated protein in the activated protein solution is immunoglobulin, lactoferrin, metallothionein, superoxides
Any one of mutase, soybean protein, lysozyme.
3. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 1, special
Sign is that the solvent of the dissolution boiled silk is LiBr aqueous solution or ethyl alcohol/CaCl aqueous solution.
4. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 1, special
Sign is, further comprising the steps of: pH adjusting agent is added to silk fibroin protein solution made from step (1) and carries out pH value adjusting,
Its pH adjusting agent added is metal ion or inorganic nanometer powder.
5. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 4, special
Sign is that the metal ion is one of calcium ion, silver ion.
6. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 4, special
Sign is that the inorganic nanometer powder is one of hydroxyapatite, tricalcium phosphate.
7. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 1, special
Sign is, the ethyl alcohol spray velocity in the ethyl alcohol spraying device is 100~1000mL/min, and concentration of alcohol used be 50~
100%.
8. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 1, special
Sign is that the fiber receiver is that fiber receives plate or fiber receives roller；When the fiber receiver is using fibre
When dimension receives plate, active fibroin fiber film is obtained；When the fiber receiver receives roller using fiber, obtain
Obtain the active fibroin albumen continuous fiber film of simple tension and one direction ordered arrangement.
9. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 1, special
Sign is, pressure-air injection stream described in step (2) is by dry compressed air, relative humidity is 10%~
50%.
10. a kind of molten spray Preparation Method of active fibroin albumen bioengineered tissue bracket according to claim 1, special
Sign is that the injection pressure in pressure-air injection stream used is 10MPa~30MPa.
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| WO2020134444A1 (en) * | 2018-12-28 | 2020-07-02 | 佛山科学技术学院 | Preparation method for biological tissue engineering stent by solution spraying |
| CN112746085A (en) * | 2020-11-16 | 2021-05-04 | 西南大学 | Method for improving mechanical property of silk fiber and product thereof |
| CN115679466A (en) * | 2022-05-20 | 2023-02-03 | 复向丝泰医疗科技(苏州)有限公司 | Silk protein pulling line and preparation method thereof |
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| ADRIÁN MAGAZ等: "Porous, Aligned, and Biomimetic Fibers of Regenerated Silk Fibroin Produced by Solution Blow Spinning", 《BIOMACROMOLECULES》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020134443A1 (en) * | 2018-12-28 | 2020-07-02 | 佛山科学技术学院 | Solution-spraying preparation method for silk fibroin biological tissue engineering stent with optimized mechanical properties |
| WO2020134444A1 (en) * | 2018-12-28 | 2020-07-02 | 佛山科学技术学院 | Preparation method for biological tissue engineering stent by solution spraying |
| CN112746085A (en) * | 2020-11-16 | 2021-05-04 | 西南大学 | Method for improving mechanical property of silk fiber and product thereof |
| CN112746085B (en) * | 2020-11-16 | 2022-06-03 | 西南大学 | Method for improving mechanical property of silk fiber and product thereof |
| CN115679466A (en) * | 2022-05-20 | 2023-02-03 | 复向丝泰医疗科技(苏州)有限公司 | Silk protein pulling line and preparation method thereof |
| CN115679466B (en) * | 2022-05-20 | 2024-01-30 | 复向丝泰医疗科技(苏州)有限公司 | Silk protein pulling wire and preparation method thereof |
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