CN1095222A - The preparation method of zymyhydrolyzed protein food - Google Patents
The preparation method of zymyhydrolyzed protein food Download PDFInfo
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- CN1095222A CN1095222A CN 93105979 CN93105979A CN1095222A CN 1095222 A CN1095222 A CN 1095222A CN 93105979 CN93105979 CN 93105979 CN 93105979 A CN93105979 A CN 93105979A CN 1095222 A CN1095222 A CN 1095222A
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Abstract
The invention provides the production technology of zymyhydrolyzed protein food, mainly is to be raw material with the soybean protein isolate, the pure protein nutraceutical that adopts the protease hydrolytic preparation easily directly to be absorbed by human body.This production process comprise material liquid preparation, the preparation of protease nutrient solution, enzyme divide separate, Separation of Solid and Liquid, sterilization and can.Technology is simple, is suitable for suitability for industrialized production.Product Normal juice is direct-edible, also can be used as raw materials for food industry, to improve protein content, also can prepare the high-protein nutrient beverage, for people's enriched food is opened up approach.
Description
The present invention relates to a kind of production technology of zymyhydrolyzed protein food, mainly is to be raw material with the vegetable protein, and enzymatic hydrolysis prepares the pure protein nutraceutical, is commonly called as delicious wine.
Protein is one of human essential nutriment, mainly contain animal protein and vegetable protein two big classes, but these protein all can not directly be absorbed by human body, must be reassembled into each proteinoid of needed by human body in vivo through digesting, resolving into each seed amino acid.And vegetable protein to obtain than animal protein more economical and cycle short, existing Chinese patent 1057165A has introduced the technological principle of Production by Enzymes, directly produces high protein rice flour from rice.So far, Shang Weijian is raw material with the soybean protein isolate, and enzyme process is produced the reported in literature that directly eats the pure protein food that absorbs for human body.
The purpose of this invention is to provide a kind of preparation method of zymyhydrolyzed protein food, technology is simple, is suitable for suitability for industrialized production; Product Normal juice can directly eat for human body and absorb, can be used as raw materials for food industry, to improve protein content and can prepare the high-protein nutrient beverage.
The object of the present invention is achieved like this: a kind of preparation method of zymyhydrolyzed protein food, this method comprises the following steps:
1. material liquid preparation
Select that to contain the soybean protein isolate (ISP) of protein more than 90% be raw material for use, under 55～65 ℃, use the sterilized water obtain solution, raw material is 1: 10 with the ratio of sterilized water;
2. protease (endopeptidase and peptide ending enzyme) nutrient solution preparation:
Getting bacterial classification AS33483 aspergillus oryzae, IFFI10081 hay bacillus and AS3374 aspergillus terricola places in the seeding tank, use the sterilized water Mixed culture, this mixed bacteria liquid is sent in the culture tank, add nitrogenous source and carbon source simultaneously, add the protease nutrient solution that sterilized water is further cultivated endopeptidase and peptide ending enzyme again from material-compound tank;
3. enzyme digestion reaction:
Described protein isolate liquid and protease nutrient solution are placed retort, 55～65 ℃, stir under, carry out enzyme digestion reaction, adding is from dipotassium hydrogen phosphate buffer control PH6.5～7.2 of material-compound tank, do not make it produce precipitation, reaction time is 24～72 hours, all decomposes with protein isolate to be as the criterion;
4. Separation of Solid and Liquid:
Adopt centrifugation to make enzymolysis mixed liquor Separation of Solid and Liquid, the solid portion of removing can be used as feed, and gained liquid partly send next process;
5. sterilization
Adopt instantaneous UHTS, 135 ℃ of temperature, pressure 3～5kg/cm
2, 2～3 seconds time;
Described raw material components contains protein 90%(butt by weight percentage) above, nitrogen soluble index 80% or more, moisture content less than 6%, fatty less than 0.5% and ash less than 4.5%.
The cultivation capacity of described seeding tank, culture tank and retort enlarges and increases progressively 10 times.
The capacity of decomposition of described protease: seeding tank 10000～15000FOlin unit, culture tank 1000～5000FOlin unit, retort 500～1000Folin unit.
The invention has the advantages that and utilize biotechnology, enzymolysis protein to make the high-protein nutritious food that is easily directly absorbed by human body, technology is simple, is suitable for suitability for industrialized production.The former meter of product contains more than the protein 18 %, and is direct-edible or as the raw material of food industry, to improve protein content, also can prepare the high-protein nutrient beverage, for people's enriched food is made contributions.
Fig. 1 is a process chart of the present invention.
Now details are as follows to the present invention with most preferred embodiment in conjunction with the accompanying drawings, but not limited thereto.
Embodiment
A kind of preparation method of enzymolysis protein food comprises the steps:
1. material liquid preparation
Get 2000g protein isolate (ISP), its component is expressed as with percentage by weight: protein (butt) 91.3%, moisture 5.2%, fat 0.42%, ash content 3.7% and crude fibre 0.5%, place material-compound tank 9, add 3～5 times of sterilized waters from aseptic water pot 5 again, continuous stirring and dissolving, add the 10000ml sterilized water then, under 60 ℃ of temperature, protein isolate is fully dissolved.
2. protease (endopeptidase and peptide ending enzyme) nutrient solution preparation:
Get 5gAS33483 aspergillus oryzae, 2gIFFI.10081 hay bacillus and 3gAS3374 aspergillus terricola, place seeding tank 1～3 to mix, add the 100ml sterilized water from aseptic water pot 5, under 55 ℃ of temperature, enzyme activity is a 13000FOlin unit, after 2 hours, mixed bacteria liquid is sent into culture tank 6～8, add the 1000ml sterilized water, cultivated 2 hours down for 55 ℃ in temperature, enzyme activity is a 3000FOlin unit, gets the protease nutrient solution.According to the different requirements of bacterial classification, can add nitrogenous source and carbon source by material-compound tank 4, for example present embodiment adds 100g sucrose, 10g sodium nitrate, 3g potassium hydrogen phosphate, 2g contain crystallization water magnesium sulfate.
3. enzyme digestion reaction:
Stir down, the protein liquid raw material of material-compound tank 9 and the nutrient solution of culture tank 6～8 are sent into retort 10～12, add 12g buffer dipotassium hydrogen phosphate from material-compound tank 9, control PH6.6, destroy the isoelectric point of protein, make it not produce precipitation, enzyme activity is 600 FOlin units, under 58 ℃ of temperature, carry out enzyme digestion reaction.Stop after 24 hours stirring, temperature is that 65 ℃, PH are 6.0, in jar supernatant is arranged, and there is 1/5 sediment at a jar end; 32 hours, temperature was 62 ℃, and PH is 6.4, and supernatant reduces, the liquid muddiness, and sediment increases; 44 hours, temperature was 63 ℃, and supernatant disappears, and sediment reduces; 56 hours, temperature was 62 ℃, and liquid phase is even, and small amount of precipitate is arranged; 68 hours, temperature was 62 ℃, and PH is 6.5, and liquid phase is even, and small amount of precipitate is arranged, obtain complete hydrolysis peptone,
Mixing material with single amino acid and thalline insoluble matter.
4. Separation of Solid and Liquid:
The mixed liquor of complete hydrolysis in the retort 10～12 is sent into centrifuge 13, carry out Separation of Solid and Liquid, remove mycelium, insoluble solids (can be used as byproduct after the sterilization and be used for feed), represent with 14 among the figure.Liquid partly be peptone,
With amino acid mixing liquid.
5. sterilization
Mixed liquor is sent into ultra high temperature short time sterilization device 15, is 5kg/cm at pressure
2, temperature is under 135 ℃, 2～3 seconds of sterilization.
Product is measured N * 6.25 yield 97.8% with Kjeldahl;
Product population 9640ml, yield 96.4%;
Impurity (mycelium sediment) 60g, 360ml;
The protein content of product is 18.26%.
Described bacterial classification is available from Wuxi enzyme preparation factory, Shanghai industrial fermentation research institute and Tianjin industrial microorganism research institute.
Claims (6)
1, a kind of preparation method of zymyhydrolyzed protein food is characterized in that described method comprises the steps:
(1). the material liquid preparation
Select that to contain the soybean protein isolate (ISP) of protein more than 90% be raw material for use, under 55～65 ℃, use the sterilized water obtain solution, raw material is 1: 10 with the ratio of sterilized water;
(2). the preparation of protease (endopeptidase and peptide ending enzyme) nutrient solution:
Getting bacterial classification AS33483 aspergillus oryzae, IFFI10081 hay bacillus and AS3374 aspergillus terricola places in the seeding tank, use the sterilized water Mixed culture, this mixed bacteria liquid is sent in the culture tank, add nitrogenous source and carbon source simultaneously, add the protease nutrient solution that sterilized water is further cultivated endopeptidase and peptide ending enzyme again from material-compound tank;
(3). enzyme digestion reaction:
Described protein isolate liquid and protease nutrient solution are placed retort, 55～65 ℃, stir under, carry out enzyme digestion reaction, adding is from dipotassium hydrogen phosphate buffer control PH6.5～7.2 of material-compound tank, do not make it produce precipitation, reaction time is 24～72 hours, all decomposes with protein isolate to be as the criterion;
(4). Separation of Solid and Liquid:
Adopt centrifugation to make enzymolysis mixed liquor Separation of Solid and Liquid, the solid portion of removing can be used as feed, and gained liquid partly send next process;
(5). sterilization
Adopt instantaneous UHTS, 135 ℃ of temperature, pressure 3～5kg/cm
2, 2～3 seconds time;
2, method according to claim 1, it is characterized in that described raw material by weight percentage, comprise: soybean protein isolate wherein contains protein 90%(butt) above, nitrogen soluble index 80% or more, moisture content less than 6%, fatty less than 0.5% and ash content less than 4.5%.
Protein 91.3%(butt), moisture 5.2%, fat 0.42%, ash content 3.7% and crude fibre 0.5% 3, according to the described method of claim 2, it is characterized in that described raw material by weight percentage, comprising:.
4, method according to claim 1 is characterized in that the capacity of decomposition of described protease in each jar is: seeding tank 10000～15000FOlin unit, culture tank 1000～5000Folin unit, retort 500～1000FOlin unit.
5, method according to claim 1 is characterized in that the cultivation capacity expansion of described seeding tank, culture tank and retort increases progressively 10 times.
6, method according to claim 1 is characterized in that described nitrogenous source and carbon source can be selected from sucrose, sodium nitrate, potassium hydrogen phosphate and contain crystallization water magnesium sulfate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93105979 CN1095222A (en) | 1993-05-19 | 1993-05-19 | The preparation method of zymyhydrolyzed protein food |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93105979 CN1095222A (en) | 1993-05-19 | 1993-05-19 | The preparation method of zymyhydrolyzed protein food |
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| CN1095222A true CN1095222A (en) | 1994-11-23 |
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| CN 93105979 Pending CN1095222A (en) | 1993-05-19 | 1993-05-19 | The preparation method of zymyhydrolyzed protein food |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1101471C (en) * | 2000-05-26 | 2003-02-12 | 黑龙江省轻工科学研究院 | Process for preparing active peptide of soybean protein |
| CN1108109C (en) * | 1999-08-03 | 2003-05-14 | 姜浩奎 | Process for extracting soybean protein |
| CN100385010C (en) * | 2003-02-25 | 2008-04-30 | 中国人民解放军军需大学 | Soybean oligopeptide production process |
| CN101658235B (en) * | 2009-08-24 | 2012-04-18 | 山东禹王实业有限公司 | Method for producing enzymolysis dairy-type isolated soy protein and product thereof |
-
1993
- 1993-05-19 CN CN 93105979 patent/CN1095222A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1108109C (en) * | 1999-08-03 | 2003-05-14 | 姜浩奎 | Process for extracting soybean protein |
| CN1101471C (en) * | 2000-05-26 | 2003-02-12 | 黑龙江省轻工科学研究院 | Process for preparing active peptide of soybean protein |
| CN100385010C (en) * | 2003-02-25 | 2008-04-30 | 中国人民解放军军需大学 | Soybean oligopeptide production process |
| CN101658235B (en) * | 2009-08-24 | 2012-04-18 | 山东禹王实业有限公司 | Method for producing enzymolysis dairy-type isolated soy protein and product thereof |
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