CN109520997A - It is a kind of based on long-range optical activity Raman detection method - Google Patents
It is a kind of based on long-range optical activity Raman detection method Download PDFInfo
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- CN109520997A CN109520997A CN201811644262.6A CN201811644262A CN109520997A CN 109520997 A CN109520997 A CN 109520997A CN 201811644262 A CN201811644262 A CN 201811644262A CN 109520997 A CN109520997 A CN 109520997A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention belongs to Systems for optical inspection fields, it discloses a kind of based on long-range optical activity Raman detection method, it is characterized in that, include the following steps: 1) to be first turned on laser (10) preheating, the laser (10) emits laser from bypass, adjusts the polarizer (7), after the polarizer (7) and reflecting mirror (5) refraction, by two to exposing to sample (1) after spectroscope (4), focusing lens (3), condenser (2), adjust ICCD spectrometer (12)；2) Raman diffused light of the sample 1 is after colour conversion/daylight filters (6), analyzer (8), coupled lens group (9), ICCD spectrometer (12) are entered by transmission fiber (11) acquisition, the spectrometer (12) is connected with host computer (13), carries out optically-active Raman detection.It can effectively improve long-range Raman detection precision, improve the resolving ability to substance.
Description
Technical field
The invention belongs to Systems for optical inspection fields, and in particular to a kind of based on long-range optical activity Raman detection method.
Background technique
Raman technology has advantage outstanding in medical domain because Raman spectrum can be used for a variety of sample forms, as solid,
Suspension, sediment etc..Increasing effect has also been played in food industry detection, it has been reported that Raman can detecte out carbon
The difference of isomer in hydrate, there has also been very big improvement for the differentiation furthermore having to present edible oil and gutter oil.It draws
Graceful technology is in terms of being also widely applied to material structure.Raman scattering spectrum is different from infrared spectroscopy, in 400cm-1 or less
Spectrogram information than infrared easy collection.So Raman technology is low with its maintenance cost, using simple, contactless to sample,
It is not damaged, the advantages that Imaging fast, simplicity, high resolution, it is widely used in physical chemistry, material, food safety, safety inspection
The fields such as survey, medicine, Gemstone Identification, forestry.
Long-range Raman spectroscopy is other than being applied to the detection of deep space substance, hazardous material detection, state outside safe distance
Anti- safety check, food monitoring and unmanned machine operation etc. have potential extensive use.
For in long-range Raman detection organic matter, it is former all to contain asymmetric carbon for almost all of compound in Living Organism
Son.Moreover, always only utilizing one of two kinds of enantiomorphisms of compound.In addition, similar compound typically belong to it is same
Kind series.For example, all monosaccharide found in living tissue actually belong to D series, and all amino acid (constitutive proteins
The basic unit of matter, except glycine) belong to L series.By carrying there is the long-range Raman system of optically-active detection system to detect
Method can effectively improve long-range Raman detection precision, improve the resolving ability to substance.
Summary of the invention
The purpose of the present invention is to solve this problem, provide it is a kind of based on long-range optical activity Raman detection method,
By the chirality of the optical activity analyzing organic substance of polarised light, organic matter is preferably distinguished, has expanded the substance detection of long-range Raman
Range and discrimination precision.
For achieving the above object, the technical scheme is that
It is a kind of based on long-range optical activity Raman detection method, which comprises the steps of: 1) be first turned on laser
10 preheatings, the laser 10 emit laser from bypass, after the polarizer 7 and reflecting mirror 5 reflect, by two to spectroscope 4,
Sample 1 is exposed to after focusing lens 3, condenser 2, adjusts ICCD spectrometer；
2) Raman diffused light of the sample 1 is after colour conversion/daylight filters 6, analyzer 8, coupled lens group 9, by transmission fiber 11
Acquisition enters ICCD spectrometer 12, and spectrometer 12 is connected with host computer 13, carries out optically-active Raman detection.
Preferably, step 1 adjusts the polarizer 7, so that laser emitting becomes linearly polarized light, linearly polarized light is through anti-
After penetrating the reflection of mirror 5, into coaxial light path system；
Preferably, wherein condenser 2, focusing lens 3, two to spectroscope 4, colour conversion/daylight filters 6, analyzer 8, coupled lens group 9
Encapsulation constitutes coaxial light path system into closed structure part.
Preferably, step 1 stops laser entering coaxial system by the optical gate that time resolution adjusts ICCD spectrometer 12
In system, when beginning reflected laser.
Preferably, step 2, viewing adjust analyzer 8 and make reflection laser peak by reflected laser at sample 1
Value reaches maximum, and at this moment 8 detection direction of analyzer and the polarizer 7 are in parallel position.
Preferably, in test sample, 8 different angle of analyzer is rotated, makes to obtain Raman spectrum peak intensity and reaches maximum value,
At this moment the rotation angle [alpha] on analyzer has all been obtained.
Compared with prior art, the beneficial effects of the present invention are:
The present invention can distinguish organic matter chiral molecules structure using remote organic analyte detection, improve detection accuracy rate, measurement side
Method measurement is reliable and stable.Have the advantages that detection range is remote, and optical detection will not generate electrostatic spark etc., can be used for personnel not
The high danger zone domain easily passed in and out.The collection optical system of use has the advantages that small in size.
Detailed description of the invention
The drawings herein are incorporated into the specification and forms part of this specification, and shows and meets implementation of the invention
Example, and be used to explain the principle of the present invention together with specification.
Fig. 1 is that the present invention is based on the schematic diagrames of long-range optical activity Raman detection method.
Specific embodiment
Below in conjunction with attached drawing, technical scheme in the embodiment of the invention is clearly and completely described.
As shown in Figure 1, it is a kind of based on long-range optical activity Raman detection method, include the following steps: 1) to be first turned on sharp
Light device 10 preheats, and the laser 10 emits laser from bypass, after the polarizer 7 and reflecting mirror 5 reflect, by two to light splitting
Sample 1 is exposed to after mirror 4, focusing lens 3, condenser 2, adjusts ICCD spectrometer；Laser preheating time is 30 minutes.
The polarizer 7 is adjusted, so that laser emitting becomes linearly polarized light, linearly polarized light is reflected through reflecting mirror 5
Afterwards, into coaxial light path system；Preferably, wherein condenser 2, focusing lens 3, two to spectroscope 4, colour conversion/daylight filters 6, inspection
Inclined device 8, the encapsulation of coupled lens group 9 constitute coaxial light path system into closed structure part.
Stop laser in entering coaxial system by the optical gate that time resolution adjusts ICCD spectrometer 12, when beginning reflects
Laser back.
2) Raman diffused light of the sample 1 is after colour conversion/daylight filters 6, analyzer 8, coupled lens group 9, by transmission light
11 acquisition of fibre enters ICCD spectrometer 12, and spectrometer 12 is connected with host computer 13, carries out optically-active Raman detection.
Viewing adjusts analyzer 8 and reflection laser peak value is made to reach maximum by reflected laser at sample 1, this
When 8 detection direction of analyzer and the polarizer 7 be in parallel position.
In test sample, 8 different angle of analyzer is rotated, makes to obtain Raman spectrum peak intensity and reaches maximum value, at this moment all
The rotation angle [alpha] on analyzer is obtained；If causing optical rotation phenomenon, keep the plane of polarised light leftward or rightward in the direction of the clock
Rotation.Rotate in a clockwise direction referred to as dextrorotation, is indicated with "+"；It rotates referred to as left-handed, is indicated with "-" counterclockwise.
Working principle are as follows: laser 10 emits laser from bypass, after the polarizer 7 and reflecting mirror 5 reflect, by two to
Sample 1 is exposed to after spectroscope 4, focusing lens 3, condenser 2；The Raman diffused light of sample 1 passes through colour conversion/daylight filters 6, analyzing
After device 8, collection system of looking in the distance, ICCD spectrometer 12 is entered by transmission fiber 11.
The estimated test target distance of this method is 50 meters.Light path system is collected using coaxial light path system, is excited
The pulse laser of gloss 532nm acquires spectrum using ICCD.Laser facula is about 1-2mm after focusing, to measurement sample size
There is no specific requirement.
Embodiments described above is only a part of the embodiment of the present invention, instead of all the embodiments.Based on this
Embodiment in invention, every other reality obtained by those of ordinary skill in the art without making creative efforts
Example is applied, shall fall within the protection scope of the present invention.
Claims (6)
1. a kind of based on long-range optical activity Raman detection method, which comprises the steps of: 1) be first turned on laser
Device (10) preheating, the laser (10) emit laser from bypass, adjust the polarizer (7), by the polarizer (7) and reflecting mirror
(5) after reflecting, by two to exposing to sample (1) after spectroscope (4), focusing lens (3), condenser (2), ICCD spectrum is adjusted
Instrument (12)；
2) Raman diffused light of the sample 1 is after colour conversion/daylight filters (6), analyzer (8), coupled lens group (9), by transmitting
Optical fiber (11) acquisition enters ICCD spectrometer (12), and the spectrometer (12) is connected with host computer (13), carries out the inspection of optically-active Raman
It surveys.
2. a kind of based on long-range optical activity Raman detection method according to claim 1, which is characterized in that wherein described poly-
Light microscopic (2), focusing lens (3), two encapsulate extremely to spectroscope (4), colour conversion/daylight filters (6), analyzer (8), coupled lens group (9)
Coaxial light path system is constituted in closed structure part.
3. a kind of based on long-range optical activity Raman detection method according to claim 1, which is characterized in that step 1 is adjusted
The whole polarizer (7), so that laser emitting becomes linearly polarized light, for linearly polarized light after reflecting mirror (5) are reflected, entrance is coaxial
In light path system.
4. a kind of based on long-range optical activity Raman detection method according to claim 1, which is characterized in that step 1 is adjusted
It saves ICCD spectrometer (12), stops laser in entering coaxial system by the optical gate that time resolution adjusts ICCD spectrometer, open
Reflected laser when the beginning.
5. a kind of based on long-range optical activity Raman detection method according to claim 1, which is characterized in that step 2 is seen
It sees by reflected laser at sample (1), adjusts analyzer (8) and reflection laser peak value is made to reach maximum, at this moment analyzing
Device (8) detection direction and the polarizer (7) are in parallel position.
6. a kind of based on long-range optical activity Raman detection method according to claim 1, which is characterized in that in test sample
When, rotation analyzer (8) different angle, which makes to obtain Raman spectrum peak intensity, reaches maximum value, has at this moment all obtained the rotation on analyzer
Gyration α.
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| CN201811644262.6A CN109520997A (en) | 2018-12-29 | 2018-12-29 | It is a kind of based on long-range optical activity Raman detection method |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6608677B1 (en) * | 1998-11-09 | 2003-08-19 | Brookhaven Science Associates Llc | Mini-lidar sensor for the remote stand-off sensing of chemical/biological substances and method for sensing same |
| US20060256334A1 (en) * | 2003-02-27 | 2006-11-16 | Werner Hug | Polarization state conversion in optically active spectroscopy |
| US20070002319A1 (en) * | 2005-04-29 | 2007-01-04 | Knopp Kevin J | Method and apparatus for conducting Raman spectroscopy |
| EP1811287A1 (en) * | 2006-01-20 | 2007-07-25 | Ecole Polytechnique | Polarimetric raman system and method for analysing a sample |
| US7336351B1 (en) * | 2006-02-07 | 2008-02-26 | Sandia Corporation | Laser remote sensing of backscattered light from a target sample |
| CN101166957A (en) * | 2004-04-30 | 2008-04-23 | 阿胡拉公司 | Method and apparatus for conducting Raman spectroscopy |
-
2018
- 2018-12-29 CN CN201811644262.6A patent/CN109520997A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6608677B1 (en) * | 1998-11-09 | 2003-08-19 | Brookhaven Science Associates Llc | Mini-lidar sensor for the remote stand-off sensing of chemical/biological substances and method for sensing same |
| US20060256334A1 (en) * | 2003-02-27 | 2006-11-16 | Werner Hug | Polarization state conversion in optically active spectroscopy |
| CN101166957A (en) * | 2004-04-30 | 2008-04-23 | 阿胡拉公司 | Method and apparatus for conducting Raman spectroscopy |
| US20070002319A1 (en) * | 2005-04-29 | 2007-01-04 | Knopp Kevin J | Method and apparatus for conducting Raman spectroscopy |
| EP1811287A1 (en) * | 2006-01-20 | 2007-07-25 | Ecole Polytechnique | Polarimetric raman system and method for analysing a sample |
| US7336351B1 (en) * | 2006-02-07 | 2008-02-26 | Sandia Corporation | Laser remote sensing of backscattered light from a target sample |
Non-Patent Citations (1)
| Title |
|---|
| 姚齐峰 等: ""基于远程拉曼光谱的物质检测研究"", 《工具技术》 * |
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