CN109517670A - A kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof - Google Patents
A kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof Download PDFInfo
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- CN109517670A CN109517670A CN201710877909.9A CN201710877909A CN109517670A CN 109517670 A CN109517670 A CN 109517670A CN 201710877909 A CN201710877909 A CN 201710877909A CN 109517670 A CN109517670 A CN 109517670A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/72—Ethers of polyoxyalkylene glycols
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/008—Polymeric surface-active agents
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/042—Acids
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/044—Hydroxides or bases
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/06—Phosphates, including polyphosphates
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2041—Dihydric alcohols
- C11D3/2044—Dihydric alcohols linear
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/34—Organic compounds containing sulfur
- C11D3/349—Organic compounds containing sulfur additionally containing nitrogen atoms, e.g. nitro, nitroso, amino, imino, nitrilo, nitrile groups containing compounds or their derivatives or thio urea
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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Abstract
The present invention relates to a kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solutions and preparation method thereof, belong to chemiluminescence immunoassay technology field.The prescription and preparation method thereof of three kinds of cleaning solutions, i.e. Abbott Laboratories' concentrated cleaning solutions, Abbott Laboratories' preexciting liquid and Abbott Laboratories' exciting liquid are contained altogether.By carrying out stringent comparison with machine effect on import sample, making cleaning solution sample by oneself has and the almost consistent effect of import commercially available product, and it is widely used in the chemical illumination immunity analysis instrument of import, it can be safely and effectively instead of sell extensively in the market and expensive import Abbott Laboratories cleaning solution.Cleaning solution configuration of the present invention is easy, low in cost, has biggish Social benefit and economic benefit.
Description
Technical field
The invention belongs to chemiluminescence immunoassay technology fields, and in particular to a kind of Abbott Laboratories' chemical illumination immunity analysis instrument
With cleaning solution and preparation method thereof.
Background technique
In the 1970s, European and American developed countries' medical field occurs with chemiluminescence immune analysis method analysis detection
Human body diseases, chemiluminescence immune analysis method combine chemiluminescence reaction and immune response, that is, have the spy of immune response
The opposite sex, and have the hypersensitivity of luminescence-producing reaction concurrently；And chemiluminescence immune analysis method is non-radioactive marker determination technology, is better than
Radioimmunoassay determination method；Chemiluminescence immune analysis method is compared with EIA enzyme immunoassay measuring method without dulling luminosity ratio
Color must comply with the limitation of Beer law；Chemiluminescence immune analysis method is compared with fluorescence immunoassay less by naturally glimmering
The interference of light；It is come out one after another based on various chemical illumination immunity analysis instrument devices after above superiority the 1990s.
Chemical illumination immunity analysis instrument can detect a variety of projects: such as cardiac marker；Tumor markers；Thyroid function；
Metabolite；Sex hormone；Transplant medicine；Curative drug；Hepatitis；Reversibility virus；Congenital disorders etc..
Chemiluminescence immune assay be the antigen directly marked with chemiluminescent agent (it is cruel such as to sting smack one's lips) or antibody with to mark
The antigen or antibody response of corresponding antibodies or antigen, magnetic graininess in this, by magnetic field the change of bonding state and free state
It learns luminous agent marker to separate, luminous promoting agent is then added in bonding state part and carries out luminescence-producing reaction, by right
The detection of bonding state luminous intensity carries out quantitative or qualitative detection.
When antigen and antibody combine in vitro, it may appear that four seed types such as agglutination, precipitating, complement combination, neutralization reaction
Seroreaction, can be widely used in research body humoral immune response, the auxiliary of the characteristic and disease of antigen and antibody
Diagnosis.But there are many factor for influencing antigen-antibody reaction, other than the property, activity and concentration by antigen-antibody itself are influenced,
Also influenced by electrolyte, pH value, the temperature etc. in chemical illumination immunity analysis instrument instrument environments.Therefore chemiluminescence immunoassay
Analysis instrument all matches cleaning solution, carries out the reaction of antigen-antibody in the liquid environment provided by cleaning solution.
The operating procedure of chemical illumination immunity analysis instrument: sample-adding incubates, washing, colorimetric.Wherein washing is that chemiluminescence is exempted from
A committed step of test success or failure is determined in epidemic disease analyzer operating process.Purpose is the unbonded immunoreactant of removal, eventually
Only antigen-antibody continuing with removing the sample ingredient unrelated with reaction and the conjugate to dissociate and adsorbed in reaction process
In the nonspecific interference substance on solid phase carrier.Otherwise blank value will be made higher.Especially nonspecific interference substance, can shadow
Ring the result of detection project.
Cleaning solution is not only that immune response provides suitable acid or alkali environment and electrolyte, it is often more important that removes reaction process
In be not associated with immunoreactant, terminate antigen-antibody continuing with, pass through the nonionic surfactant in buffer, specificity
It is chemically reacted with unbonded immunoreactant and the nonspecific interference substance being adsorbed on solid phase carrier, to remove this
The immune impurity of a little interference guarantees that chemiluminescence immunoassay inspection can be normally carried out.
The chemical illumination immunity analysis instrument that domestic hospitals use at present is usually using the mating cleaning liquid series of import, such as beauty
The original-pack cleaning solution used of Abbott Laboratories, state mono- 2000 chemical illumination immunity analysis instruments of i, but the cleaning solution of the imported with original packaging is expensive,
The burden for increasing user is unfavorable for promoting the use of.
Summary of the invention
For the disadvantage for overcoming the original-pack cleaning solution of Abbott Laboratories' chemical illumination immunity analysis instrument expensive, the present invention provides one kind
Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof, this method is low in cost, and market sale price is lower, and
By computer experiment, can reach with the consistent effect of the original-pack reagent of import, the needs of medical field can be met, have wide city
Field prospect and potential value.
A kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof, it is characterised in that be concentrated including Abbott Laboratories
The prescription of three kinds of cleaning solutions and preparation method thereof including cleaning solution, Abbott Laboratories' preexciting liquid and Abbott Laboratories' exciting liquid.
Described Abbott Laboratories' concentrated cleaning solutions and preparation method thereof, it is characterised in that Abbott Laboratories' concentrated cleaning solutions are a kind of aqueous solutions
Composition includes following components:
The component of Abbott Laboratories' concentrated cleaning solutions, it is characterised in that potassium dihydrogen phosphate and sodium hydroxide are mainly used for making molten
Liquid keeps certain ionic strength, makes it have certain conductivity and suitable pH value；Propylene glycol is cosolvent, can make the water
Solution keeps higher osmotic pressure；Triton x-100 is surfactant, and the aqueous solution can be made to have cleaning action；Preservative
CY-1 can extend its storage time, and its price is also far below preservative and iterates sodium, further reduced chemiluminescence of the present invention
The preparation cost of immunity analysis instrument concentrated cleaning solutions.
Described Abbott Laboratories' preexciting liquid and preparation method thereof, it is characterised in that Abbott Laboratories' preexciting liquid is a kind of aqueous solution combination
Object includes following components:
H2O2 44ml/L
HNO3 0.85g/L
Triton x-100 0.275ml/L
The component of Abbott Laboratories' preexciting liquid, it is characterised in that nitric acid is mainly used for adjusting the pH of the system, makes its tool
There is certain acidity；H2O2With strong oxidizing property, used as oxidant；Triton x-100 is surfactant, can make the water
Solution has cleaning action.The main function of the reagent is to be cleaved marker from reaction compound, and provide one
Acid environment prevents reaction from occurring to early.
Described Abbott Laboratories' exciting liquid and preparation method thereof, it is characterised in that Abbott Laboratories' exciting liquid is a kind of aqueous solution composition,
Include following components:
NaOH 0.35mol/L
Triton x-100 43ml/L
The component of Abbott Laboratories' exciting liquid, it is characterised in that NaOH is mainly used for that solution is made to have certain ion strong
Degree makes it have constant conductivity and solution is made to have stronger alkalinity.The product is for providing alkaline environment, excitation chemistry
Luminescence-producing reaction.
The beneficial effects of the present invention are:
1. cleaning solution of the invention, configuration is easy, low in cost, the chemiluminescence immunoassay suitable for import brands such as Abbott Laboratories
Analyzer.
2. self-control cleaning solution sample has and import commercially available product by carrying out stringent comparison with machine effect on import sample
Intimate consistent effect, and it is widely used in the chemical illumination immunity analysis instrument of import, it can safely and effectively replace in the market
Import sell extensively and expensive Abbott Laboratories cleaning solution is used to effectively solve prior art chemical illumination immunity analysis instrument
Buffer relies on the status of import for a long time.
Specific embodiment
For a better understanding of the invention, it further illustrates combined with specific embodiments below.
Embodiment 1
The preparation method of concentrated cleaning solutions of the invention includes the following steps: to launch potassium dihydrogen phosphate 1.02mol/L
Propylene glycol (15ml/ is added in (138.8118g) and sodium hydroxide 0.48mol/L (19.2g) stirring and dissolving into 1L deionized water
L) stirring and dissolving, add preservative CY-1 (2ml/L) stirring and dissolving, be eventually adding triton x-100 (5ml/L) and stir,
PH=6.48 makes pH=6.8 with certain density NaOH solution tune pH.
The concentrated cleaning solutions of the present invention of table 1 are compared with the physical and chemical index of original-pack reagent and domestic reagent
Embodiment 2
The preparation method of preexciting liquid of the invention includes the following steps: accurately to weigh H2O2(44ml)、HNO3(0.85g)、
Triton x-100 (0.275ml/L) is added in one liter of deionized water, stirs evenly.
The preexciting liquid of the present invention of table 2 is compared with the physical and chemical index of original-pack reagent
Embodiment 3
The preparation method of exciting liquid of the invention includes the following steps: that NaOH 0.35mol/L is added in 1L deionized water and stirs
Dissolution is mixed, triton x-100 43ml/L stirring is added.
The exciting liquid of the present invention of table 3 is compared with the physical and chemical index of original-pack reagent
Table 4 makes cleaning solution by oneself compared with imported with original packaging Abbott Laboratories cleaning liquid series carry out upper machine
Above-mentioned items are the experimental results showed that cleaning solution of the present invention and import reagent testing result can be substituted without significant difference
Original-pack reagent.
Embodiments of the present invention are only expressed above, and the description thereof is more specific and detailed, but can not therefore understand
For the limitation to patent of invention range.It should be pointed out that for those of ordinary skill in the art, not departing from this hair
Under the premise of bright design, various modifications and improvements can be made, and these are all within the scope of protection of the present invention.Therefore, this hair
The scope of protection shall be subject to the appended claims for bright patent.
Claims (7)
1. a kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof, it is characterised in that clear including Abbott Laboratories' concentration
The prescription of three kinds of cleaning solutions and preparation method thereof including washing lotion, Abbott Laboratories' preexciting liquid and Abbott Laboratories' exciting liquid.
2. Abbott Laboratories' concentrated cleaning solutions according to claim 1 and preparation method thereof, it is characterised in that Abbott Laboratories' concentrated cleaning solutions
It is a kind of aqueous solution composition, includes following components:
3. the component of Abbott Laboratories' concentrated cleaning solutions according to claim 2, it is characterised in that potassium dihydrogen phosphate and sodium hydroxide
It is mainly used for the ionic strength for making solution keep certain, makes it have certain conductivity and suitable pH value；Propylene glycol is to help
Solvent can make the aqueous solution keep higher osmotic pressure；Triton x-100 is surfactant, the aqueous solution can be made to have clear
The effect of washing；Preservative CY-1 can extend its storage time, and its price is also far below preservative and iterates sodium, further reduced this
The preparation cost of invention chemical illumination immunity analysis instrument concentrated cleaning solutions.
4. Abbott Laboratories' preexciting liquid according to claim 1 and preparation method thereof, it is characterised in that Abbott Laboratories' preexciting liquid is one
Kind aqueous solution composition, includes following components:
H2O2 44ml/L
HNO3 0.85g/L
Triton x-100 0.275ml/L
5. the component of Abbott Laboratories' preexciting liquid according to claim 4, it is characterised in that nitric acid is mainly used for adjusting the system
PH, make it have certain acidity；H2O2With strong oxidizing property, used as oxidant；Triton x-100 is surface-active
Agent can make the aqueous solution have cleaning action.The main function of the reagent is to be cleaved marker from reaction compound,
And providing an acid environment prevents reaction from occurring to early.
6. Abbott Laboratories' exciting liquid according to claim 1 and preparation method thereof, it is characterised in that Abbott Laboratories' exciting liquid is a kind of water
Liquid composite includes following components:
NaOH 0.35mol/L
Triton x-100 43ml/L
7. the component of Abbott Laboratories' exciting liquid according to claim 6, it is characterised in that NaOH is mainly used for that solution is made to have one
Fixed ionic strength makes it have constant conductivity and solution is made to have stronger alkalinity.The product is for providing alkaline ring
Border excites chemiluminescence reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710877909.9A CN109517670A (en) | 2017-09-20 | 2017-09-20 | A kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710877909.9A CN109517670A (en) | 2017-09-20 | 2017-09-20 | A kind of Abbott Laboratories' chemical illumination immunity analysis instrument cleaning solution and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110568183A (en) * | 2019-09-18 | 2019-12-13 | 潍坊市康华生物技术有限公司 | substrate solution for chemiluminescence immunoassay analyzer and preparation method thereof |
| CN111607465A (en) * | 2020-06-08 | 2020-09-01 | 珠海丽珠试剂股份有限公司 | Chemiluminescent cleaning solution and preparation method and application thereof |
| CN114410390A (en) * | 2022-01-25 | 2022-04-29 | 武汉艾迪康医学检验所有限公司 | Cleaning solution for chemiluminescence reaction system and preparation method thereof |
| CN115404131A (en) * | 2021-05-28 | 2022-11-29 | 深圳市帝迈生物技术有限公司 | Cleaning liquid |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110568183A (en) * | 2019-09-18 | 2019-12-13 | 潍坊市康华生物技术有限公司 | substrate solution for chemiluminescence immunoassay analyzer and preparation method thereof |
| CN111607465A (en) * | 2020-06-08 | 2020-09-01 | 珠海丽珠试剂股份有限公司 | Chemiluminescent cleaning solution and preparation method and application thereof |
| CN115404131A (en) * | 2021-05-28 | 2022-11-29 | 深圳市帝迈生物技术有限公司 | Cleaning liquid |
| CN114410390A (en) * | 2022-01-25 | 2022-04-29 | 武汉艾迪康医学检验所有限公司 | Cleaning solution for chemiluminescence reaction system and preparation method thereof |
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Application publication date: 20190326 |