CN109507248A - With the method for glucose content in Instrument for Measuring Blood Sugar measurement fructus lycii - Google Patents
With the method for glucose content in Instrument for Measuring Blood Sugar measurement fructus lycii Download PDFInfo
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- CN109507248A CN109507248A CN201811520815.7A CN201811520815A CN109507248A CN 109507248 A CN109507248 A CN 109507248A CN 201811520815 A CN201811520815 A CN 201811520815A CN 109507248 A CN109507248 A CN 109507248A
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Abstract
The invention discloses a kind of method with glucose content in Instrument for Measuring Blood Sugar measurement fructus lycii, this method measures extracting solution pol value using different sources fructus lycii medicinal material as raw material, with blood glucose meter.When concentration of glucose is within the scope of 0.401 ~ 2.003mg/ml, pol value and its concentration have good linear relationship: Y=6.5654X-0.2116, r=0.9967.Mean sample recovery rate is 91.1%, RSD 4.5%(n=6).The present invention is practical with the method that Instrument for Measuring Blood Sugar measures glucose content in fructus lycii, and sensitive, quick, easy, precision, repeatability, stability result are reliable, easy to operate, without using reagent, and simple economy can be further applicable in practice.
Description
Technical field
It a kind of is measured the present invention relates to a kind of method of glucose content in measurement fructus lycii more particularly to Instrument for Measuring Blood Sugar
The method of glucose content in fructus lycii.
Background technique
Fructus lycii is Lycium barbarum L. of solanaceaeLyciumbarbarumL. dry mature fruit has nourishing liver and kidney, benefit
The effect of shrewd head, adjust immune, anti-aging, it is antitumor, reduce blood lipid and in terms of show wide application before
Scape, main product are the important characteristics in western China in arid and semi-arid lands such as Ningxia, Qinghai, Gansu, Inner Mongol, Xinjiang
One of resources of medicinal plant.In recent years, with to fructus lycii chemical component and bioactivity recognize and research go deep into, Yi Jiren
Health care consciousness reinforcement, the development and utilization of fructus lycii are no longer confined to its medical value, and it is extensive to be more conceived to fructus lycii
Bioactivity.In the research for having focused largely on its physiological activity and polysaccharide composition to the research of fructus lycii both at home and abroad.Polysaccharides
(Lyciumbarbarum polysaccharides, LBPs) is one of most important active constituent of fructus lycii, but polysaccharide biology is living
Property is closely related with many factors such as its physicochemical properties, such as dissolubility, molecular weight, space structure.Due to primary structure
It is the basis of space structure, glucose is the main component for forming polysaccharide primary structure.Glucose is as the main water-soluble of fructus lycii
Property ingredient, it is necessary to find a kind of method quick, easy to operate and determine its glucose content, thus as the quick area of marker
Divide the fructus lycii sample of different sources, different cultivars.
Summary of the invention
The object of the present invention is to provide a kind of methods with glucose content in Instrument for Measuring Blood Sugar measurement fructus lycii.
One, in fructus lycii glucose content measurement
The method that the present invention measures glucose content in fructus lycii with Instrument for Measuring Blood Sugar, comprising the following steps:
The preparation of sample solution: fructus lycii is crushed, and crosses 30 meshes；It is added to the water by 0.01 ~ 0.05g/mL, in 50 ~ 90 DEG C
Stirred in water bath extract 0.5 ~ 2 hour, be cooled to room temperature, be centrifuged to obtain extracting solution；Extracting solution is diluted with water again；Amount of water is
8 ~ 12 times of extracting liquid volume；
Measuring method: using the pol value of blood glucose meter measurement sample solution, and according to the pol value of sample solution and its glucose
Glucose content is calculated in the equation of linear regression of concentration.
Two, the equation of linear regression of the pol value of solution and its concentration of glucose
1 materials and methods
1.1 materials and reagent
Fructus lycii raw material picks up from the ground such as Ningxia, Qinghai, Gansu, Inner Mongol, Xinjiang, by Lanzhou Inst. of Chemical Physics, Chinese Academy of Sciences's residence of a high official
Mostly grand research institute is accredited asLycium barbarum L.。
Test chemical plant, lot number: 20160228 in D- DEXTROSE ANHYDROUS (analysis is pure) Laiyang；Experimental water is ultrapure water, by
Water purification machine is made.
1.2 instrument and equipment
The Shanghai BS 224S type a ten thousandth electronic balance Sai Duolisi trade Co., Ltd；The stable Instrument for Measuring Blood Sugar Changsha of three promises
Three promise biosensor technique Co., Ltds；CLK05A type centrifuge Hunan Kai Da scientific instrument Co., Ltd；101-1A type electric heating
The Yongxing Ke Wei, air dry oven Beijing Instrument Ltd.；The examination of the type electronic thermostatic stainless steel water bath Shanghai HHS-2S east star building materials
Test equipment Co., Ltd；Believe strong board multifunctional high speed pulverizer Shanghai Guang Sha Trade Co., Ltd.；JJ-1 type precision reinforcement is electronic
Blender Changzhou Guohua Electric Appliance Co., Ltd.；The excellent general UPS series pure water system Chengdu Ultra Pure Science & Technology Co., Ltd of II type of UPS-.
2 methods and result
The preparation of 2.1 test solutions
It after fructus lycii crushed 30 meshes, mixes well, takes about 2.0 g of this product, it is accurately weighed, in 250 mL round-bottomed flasks
In, add 100 mL of water, extracts 2 hours (stirring rate 120rpm) in 60 DEG C of stirring in water bath, let cool to room temperature, be centrifuged to obtain extracting solution
(4000 rpm of centrifugation rate), precision measure extracting solution 5mL be diluted with water to scale in 50 mL measuring bottles, shake up to get.
2.2 pol values determination methods
Pol value measuring principle is that glucose is anti-with potassium ferricyanide generation redox under the catalysis of glucose oxidase (GOD)
It answers, generates gluconic acid and potassium ferrocyanide, electrochemical reaction occurs for potassium ferrocyanide, generates redox current.Oxidation is also
The size of primary current and the concentration of glucose are directly proportional.Blood glucose meter show that glucose is dense by measuring the size of redox current
The numerical value of degree.
In measurement, the correcting code for needing first to adjust together is consistent with the correcting code in test strips outer packing, surveys at room temperature
It is fixed.
The drafting of 2.3 standard curves
Precision weighs dry D- DEXTROSE ANHYDROUS (analysis is pure) 1.0017g to constant weight and is dissolved in water simultaneously in 100mL measuring bottle
It is diluted to scale, is shaken up, the stock solution (every 10.017 mg of 1mL DEXTROSE ANHYDROUS containing D-) as contrast solution.Precision measures
Stock solution 2.0 mL, 4.0 mL, 6.0 mL of above-mentioned contrast solution, 8.0 mL, 10.0 mL are respectively placed in 50 ml measuring bottles,
It is diluted with water to scale, is shaken up, the glucose standard curve work of 0.401,0.801,1.202,1.603,2.003 mg/mL is made
Make liquid.The pol value measured using blood glucose meter as ordinate (Y, unit: mmol/L), concentration of glucose be abscissa (X, unit:
Mg/mL standard curve) is drawn, glucose regression equation: Y=6.5654X-0.2116, r=0.9967 are obtained.Show that glucose is dense
For degree within the scope of 0.401 ~ 2.003mg/mL, pol value and its concentration have good linear relationship.
2.4 precision test
The glucose standard curve working solution of the 1.202mg/mL under " 2.3 " item is taken, 5 pol values of replication measure sugar
Angle value is respectively as follows: 7.2,7.3,7.3,7.2,7.4, RSD=1.1%(n=5), precision is good.
2.5 repetitive test
5 parts of sample, every part of about 10g of same batch (ZNZT1) is taken, by legal system available test sample solution below " 2.1 " item, replication
5 pol values measure pol value and are respectively as follows: 5.6,5.6,5.8,5.8,5.7, RSD=1.8%(n=5), repeatability is good.
2.6 stability experiment
It takes sample (ZNZT1) under " 2.5 " item a, is placing 0,2,4,6,8h, measuring pol value respectively, be as a result respectively as follows:
5.6,5.8,5.5,5.7,5.7, RSD=2.1%, show to handle sample with this method, pol value is stablized in 8 h.
2.7 sample-adding recovery tests
Taking known sugars angle value is 6 parts of sample of 5.7 sample (ZNZT1), and respectively about 5g, accurately weighed, and glucose pair is added in precision
According to the stock solution 50mL of solution, sample solution then is prepared by the preparation method of test solution under " 2.1 " item, is measured respectively
Its pol value.Calculate glucose average recovery rate be 91.1%, RSD 4.5%(n=6).It the results are shown in Table 1:
。
This experiment establishes the side with glucose content in blood glucose meter measurement fructus lycii using different sources fructus lycii medicinal material as raw material
Method, and methodological study has been carried out, the results show that precision, repeatability, stability result are reliable, concentration of glucose is 0.401
Within the scope of ~ 2.003mg/ml, pol value and its concentration have good linear relationship.
This experiment is practical with the method that blood glucose meter measures glucose content in fructus lycii, and method is sensitive, quick, operation is simple
Just, without using reagent, simple economy, to establish analysis method of the carbohydrate content glucose as fructus lycii carbohydrate marker, into
One step promotes and applies in practice provides reference.
Pol value measurement result shows that the Lycium berry and grape sugared content difference of different regions is larger, and glucose is medlar biological
The Primary product of metabolism, the factors such as content and the height above sea level of growing environment, soil nutrient, moisture, weather are related.There is sample segment
There are certain deviations, but in view of medicinal material itself belongs to natural products, there are many non-determined influence factors, just often occur so belonging to
As.This experimental studies results can provide scientific basis for the selection of scientific evaluation fructus lycii medicinal material inherent quality, high-quality medicinal material.
Detailed description of the invention
Fig. 1 is the standard curve of D- DEXTROSE ANHYDROUS.
Specific embodiment
It is described further below by method of the specific embodiment to glucose content in present invention measurement fructus lycii.
Sample solution is prepared: taking the sample of fructus lycii 23 batches of peaceful Qi 1.After fructus lycii crushed 30 meshes, mix well,
About 2.0 g of this product is taken, it is accurately weighed, in 250 mL round-bottomed flasks, add 100 mL of water, is extracted 2 hours in 60 DEG C of stirring in water bath
(stirring rate 120rpm) is let cool to room temperature, is centrifuged to obtain extracting solution (4000 rpm of centrifugation rate), and precision measures extracting solution 5mL,
In 50 mL measuring bottles, be diluted with water to scale, shake up to get.
Measuring method: the pol value of sample solution is measured by blood glucose meter, and pol value brings calibration curve equation calculating into
Glucose content.No. 1 23 batches of sample measurement results of peaceful Qi are as shown in table 2:
Claims (3)
1. with the method for glucose content in Instrument for Measuring Blood Sugar measurement fructus lycii, comprising the following steps:
The preparation of sample solution: fructus lycii is crushed, and crosses 30 meshes；It is added to the water by 0.01 ~ 0.05g/mL, in 50 ~ 90 DEG C
Stirred in water bath extract 0.5 ~ 2 hour, be cooled to room temperature, be centrifuged to obtain extracting solution；Extracting solution is diluted with water again；Amount of water is
8 ~ 12 times of extracting liquid volume；
Measuring method: using the pol value of blood glucose meter measurement sample solution, and according to the pol value of sample solution and its glucose
Glucose content is calculated in the equation of linear regression of concentration；
Within the scope of 0.401 ~ 2.003mg/mL, pol value and the linear of concentration of glucose return the concentration of glucose of sample solution
Return equation as follows:
Y=6.5654X-0.2116, r=0.9967
Y --- pol value；Unit: mmol/L
X --- concentration of glucose, unit: mg/mL.
2. as described in claim 1 with the method for glucose content in Instrument for Measuring Blood Sugar measurement fructus lycii, it is characterised in that: described to stir
Mixing rate is 80 ~ 120rpm.
3. as described in claim 1 with Instrument for Measuring Blood Sugar measurement fructus lycii in glucose content method, it is characterised in that: it is described from
Heart rate is 3000 ~ 5000 rpm.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349327A (en) * | 2015-12-14 | 2016-02-24 | 江南大学 | Real-time control method of sugar content and alcohol content in wine mash during yellow rice wine primary fermentation process |
| CN105628861A (en) * | 2016-02-03 | 2016-06-01 | 齐齐哈尔大学 | Method for quickly measuring content of vitamin C in Fructus lycii |
| CN106770991A (en) * | 2017-03-28 | 2017-05-31 | 农业部环境保护科研监测所 | A kind of method for determining zymotic fluid sugar content and its application |
| JP2017129423A (en) * | 2016-01-19 | 2017-07-27 | テルモ株式会社 | Component measurement device |
| CN108717086A (en) * | 2018-05-03 | 2018-10-30 | 山西大学 | A kind of method of gluconic acid content in measurement food |
-
2018
- 2018-12-12 CN CN201811520815.7A patent/CN109507248A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349327A (en) * | 2015-12-14 | 2016-02-24 | 江南大学 | Real-time control method of sugar content and alcohol content in wine mash during yellow rice wine primary fermentation process |
| JP2017129423A (en) * | 2016-01-19 | 2017-07-27 | テルモ株式会社 | Component measurement device |
| CN105628861A (en) * | 2016-02-03 | 2016-06-01 | 齐齐哈尔大学 | Method for quickly measuring content of vitamin C in Fructus lycii |
| CN106770991A (en) * | 2017-03-28 | 2017-05-31 | 农业部环境保护科研监测所 | A kind of method for determining zymotic fluid sugar content and its application |
| CN108717086A (en) * | 2018-05-03 | 2018-10-30 | 山西大学 | A kind of method of gluconic acid content in measurement food |
Non-Patent Citations (4)
| Title |
|---|
| 朱冠琳等: "血糖仪快速测定豆类中葡萄糖含量方法", 《安徽农学通报》 * |
| 胡嘉鹏: "使用血糖仪测定酱油中的葡萄糖 ", 《中国酿造》 * |
| 谭荧飞等: "利用便携式血糖仪测定酱油中的葡萄糖 ", 《食品科技》 * |
| 陈佛兰等: "血糖仪法快速测定禽蛋中的葡萄糖 ", 《科技风》 * |
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Application publication date: 20190322 |