CN109504525A - It is a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction - Google Patents

It is a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction Download PDF

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Publication number
CN109504525A
CN109504525A CN201811449992.0A CN201811449992A CN109504525A CN 109504525 A CN109504525 A CN 109504525A CN 201811449992 A CN201811449992 A CN 201811449992A CN 109504525 A CN109504525 A CN 109504525A
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dna
aflatoxin
grease
oil
minutes
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李军生
阎柳娟
黄国霞
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Guangxi University of Science and Technology
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Guangxi University of Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/16—Refining fats or fatty oils by mechanical means

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Microbiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction, i.e. using DNA, covalently aflatoxin-DNA addition product is stablized in chimeric formation with aflatoxin, then removes wherein DNA by separation and reach the method for being simple and efficient and removing edible oil and fat aflatoxin.This method is under the premise for guaranteeing DNA maintenance duplex structure, washing process is refined in conjunction with conventional grease, directly DNA solution is added among the grease by aflatoxin contamination according to a certain percentage, it is sufficiently mixed, and by improving temperature, adjustment ionic strength, adjustment pH value etc., promote aflatoxin and DNA to be combined into stable aflatoxin-DNA addition product, then wherein DNA is removed by water-oil separating technique and removes wherein remaining aflatoxin.This method and traditional edible oil and fat aflatoxin removing method are entirely different, are particularly suitable for the oil and fat refining detoxification of residual traces aflatoxin.

Description

It is a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction
Technical field
The present invention relates to a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction.
Background technique
Aflatoxin, mainly by aspergillus flavus (Aspergillus flavus) or aspergillus parasiticus (Aspergillus Arasiticus) secretion generates, and is the strongest natural materials of carcinogenicity generally acknowledged at present, is ground by World Health Organization's international cancer Study carefully general administration (IARC) to incorporate into as I class carcinogen, there is very high hepatotoxicity wind agitation, tumorigenicity, teratogenesis and mutagenicity.The mankind Nearly all agricultural product, including cereal, peanut, corn and soybean for depending on for existence etc., links before, during and after harvesting, Even it is included in production, processing, is likely to because breeding aspergillus flavus (Aspergillus flavus) or parasitic in storage process Aspergillus (Aspergillus arasiticus) and it is aflatoxin-contaminated, wherein again with peanut and corn be easiest to breed phase The mould answered, peanut oil and corn oil are most susceptible to aflatoxin contamination.There is an urgent need to eliminate aspergillus flavus poison as much as possible Adverse effect of the element to world economy and human health.
And tradition eliminates aflatoxin method, such as: extraction uses ethyl alcohol, acetone, isopropanol, cyclohexyl first The organic solvents such as alcohol extract oil plant seed or raw material, can remove aflatoxin, but the high cost and toxicity extraction of this kind of method Solvent waste disposal issues are taken to limit the popularization and application of the technology；Absorption method passes through activated carbon, activated silica diatomaceous earth, boiling The adsorbents Adsorption aflatoxin such as stone, but abatement Effect value must improve；Chemical removal method, i.e., by alkali, such as: hydroxide Sodium, ammonium hydroxide, calcium hydroxide etc. open aflatoxin lactonic ring, form cumarin sodium salt or ammonium salt and promote aspergillus flavus malicious Element disappears, or by oxidant, such as: sodium hypochlorite, hydrogen peroxide, chlorine, ozone make aflatoxin lose toxicity, still These methods are possible to generate new noxious material, it could even be possible to change grease acid value or peroxide value and influence grease production Product quality；Biological removal method, is still in the experimental exploring stage, and many technical methods still cannot be directly used to edible plant at present The refining detoxification of object grease.The equal Shortcomings of these conventional methods or defect.
After existing the experimental results show that aflatoxin enters body, in the catalytic action of cytochrome P 450 enzymes Under be converted to after the epoxides of activation and the intercalation of DNA and aflatoxin-DNA addition product could be combined into DNA.Just because of Aflatoxin is by aflatoxin B1Outer formula -8,9- epoxides intercalation of DNA simultaneously forms aflatoxin-DNA with DNA Addition product causes DNA to be crosslinked, and base-pair replacement or frameshift mutation to influence the normal replication and transcription of DNA, or even cause Base transition or loss, eventually lead to gene mutation, lead to tumor suppressor gene inactivation and/or protooncogene activation, eventually lead to cancer The generation of disease is (referring to document Wang M L, Yu Y Y, Liang C, Lu A P, Zhang G. Recent advances in developing small molecules targeting nucleic acid. Int. J. Mol. Sci., 2016,17:779-802).
Aflatoxin B1 addition product structure:
Summary of the invention
The technical problem to be solved by the present invention is the present invention provides a kind of grease aflatoxin based on DNA packing interaction Removing method is captured, i.e., directly adds corresponding DNA solution into edible oil and fat to be processed, passes through aflatoxin and DNA After packing interaction occurs, then DNA is removed by separation and captures the method for eliminating grease aflatoxin.This method and tradition are eaten It is entirely different with grease aflatoxin removing method, it is particularly suitable for the oil and fat refining detoxification of residual traces aflatoxin.
The technical solution for solving above-mentioned technical problem is: it is a kind of based on the grease aflatoxin of DNA packing interaction prisoner Removing method is obtained, this method is to make full use of hydrophilic large biological molecule DNA, and DNA is enable to be total to the aflatoxin in grease Valence is chimeric to form stable aflatoxin-DNA addition product, then removes wherein DNA by water-oil separating technique and remove in grease Remaining aflatoxin.
Further, the washing process refined by traditional edible oil and fat, in traditional washing process link or tradition washing After process procedure, directly DNA solution is added among the grease by aflatoxin contamination according to a certain percentage, it is sufficiently mixed It closes, and by improving temperature, adjustment ionic strength and adjustment pH value, promotes the aflatoxin intercalation of DNA, promote aflatoxin It is combined into stable aflatoxin-DNA addition product with DNA, then wherein DNA is removed by water-oil separating technique and is removed wherein Remaining aflatoxin.
Further, comprising the following steps:
(1) DNA is dissolved in the buffer being made of phosphate, glycine, sodium hydroxide, sodium carbonate or sodium bicarbonate, is obtained To DNA buffer solution, in 7-10 range, ionic strength is controlled in 50mM-200mM range for the pH value control of DNA buffer solution, DNA concentration is controlled in 0.5-5% range；
(2) aflatoxin content detection is carried out in advance to aflatoxin contamination oil sample；
(3) ratio of -800 microgram aflatoxin of 100 microgram is combined to calculate according to 1 gram of DNA insertion, in traditional washing process ring After section or traditional washing process link, DNA buffer solution prepared by step (1) is added among grease, is added into grease Add with step (1) identical buffer as water lotion, makes the 5-10% of buffer total amount grease total weight in grease；
(4) it is sufficiently mixed, incorporation time maintains -60 minutes 30 minutes, and temperature maintains 50 DEG C -70 DEG C；
(5) it after handling, is allowed to stand at room temperature for 30 minutes -120 minutes, then through oil water separator or the oil that is centrifuged at a high speed Water obtains grease；
(6) grease obtained again through pure water washing 1-2 times, washing water additive amount accounts for the 5%-10% of oil quality, is sufficiently mixed, and mixes Time maintenance -60 minutes 30 minutes is closed, temperature maintains 50 DEG C -70 DEG C, is allowed to stand at room temperature for 30 minutes -120 minutes, then passes through Oil water separator or the grease that is centrifuged at a high speed, obtain purification grease.
The DNA is calf thymus DNA, herring sperm dna, salmon sperm dna, cerevisiae dna or cauliflower DNA.
In fact, there are also documents to show that is, pure in vitro test, aflatoxin also can be embedding even if under the conditions of non-enzymatic Enter DNA, and forms stable aflatoxin-DNA addition product with DNA covalent bond.Therefore, we completely can be by yellow bent The mould toxin intercalation of DNA, and stable aflatoxin-DNA addition product is formed with DNA covalent bond, it is then clear by separation again Achieve the purpose that be simple and efficient except wherein DNA and removes aflatoxin.Since DNA is hydrophilic large biological molecule, it borrows Help the washing process of traditional edible oil and fat refining, and by directly adding among appropriate DNA to corresponding washing lotion, promote DNA and The remaining abundant covalent bond of aflatoxin in grease, then remove water solubility DNA by water-oil separating technique and eliminate edible Aflatoxin in grease.Aflatoxin of the present invention eliminates principle and technical method and traditional edible oil and fat aspergillus flavus Toxin removing method is entirely different.
Since aflatoxin and is formed with duplex structure DNA covalent bond stable by being embedded in duplex structure DNA Engomphosis relation is not present with unwound DNA or single stranded DNA in aflatoxin-DNA addition product, and at least engomphosis relation is not close, because This, where the key problem in technology for maintaining DNA double chain stable structure to be effectively chimeric capture aflatoxin of the invention, therefore, at this In invention implementation process, on the one hand, aflatoxin can be promoted to be embedded in duplex structure DNA, and and double-strand by improving temperature Structural DNA covalent bond forms stable aflatoxin-DNA addition product, but on the other hand, double-strand can also be made by improving temperature Structural DNA unwinding becomes single stranded DNA and loses the function of being effectively fitted into aflatoxin, and usual DNA melting temperature is generally 70 Between DEG C -85 DEG C.Therefore, improving temperature can promote aflatoxin to be embedded in duplex structure DNA, and total with duplex structure DNA Valence, which combines, forms stable aflatoxin-DNA addition product, but in order to avoid duplex structure DNA unwinding is sent out as single stranded DNA Raw, reaction temperature controls between 50 DEG C -70 DEG C.Technical principle of the invention hair related to existing aflatoxin aptamer Bright technical principle is entirely different, and aptamer involved in aflatoxin aptamer related invention refers to a series of single-stranded Nucleic acid molecules are combined with the identical specific target molecules of length, and aflatoxin is specifically suitable with nucleic acid as synantibody Body combines.The technical principle for eliminating aflatoxin with traditional absorption method is also entirely different.
Other than temperature influences, pH value also has a major impact the stable structure of DNA.On the one hand, ring of the DNA in meta-alkalescence It is relatively stable in border, in acid condition, DNA be easy it is hydrolyzed, on the other hand, when the pH value of solution is within the scope of 5-10, DNA Melting temperature variation is unobvious, and when pH>11 or pH<4, the variation of DNA melting temperature is obvious.Therefore, the present invention emphasizes that pH value is answered Control is in 7-10 range.In addition, ionic strength increases, increases the polarity of DNA surrounding medium, increase the hydrophobic effect of base-pair Power, therefore will increase the stability of DNA.In addition, ionic strength increases, aflatoxin and the mode of action of DNA can also be made Embeddeding action mode is become from electrostatic interaction.Therefore, suitably enhance ionic strength, be conducive to DNA structure and stablize, may additionally facilitate Huang Aspertoxin is embedded in duplex structure DNA, and forms stable aflatoxin-DNA addition with duplex structure DNA covalent bond Object, for this purpose, ionic strength control is in 50mM-200mM range.By preparing the buffer solution of different pH value, adjustable environment Ionic strength.Since phosphate, glycine, sodium hydroxide, sodium carbonate, sodium bicarbonate etc. can be used for food production processing Compound, therefore, buffer solution of the present invention and ionic strength adjust should by phosphate, glycine, sodium hydroxide, Sodium carbonate, sodium bicarbonate etc. are constituted or are realized.
Existing test result shows that aflatoxin is about micro- with 0.0001-0.0008 micromole aflatoxin/1 The ratio of mol nucleotide is embedded in duplex structure DNA, and forms stable aflatoxin-with duplex structure DNA covalent bond DNA addition product, since the molecular weight of aflatoxin and nucleotide is very close, the present invention is in advance to yellow bent grease Sample carries out on the basis of aflatoxin content detection, and according to every -800 microgram aflatoxin/1 gram DNA of 100 microgram Ratio add DNA.
Specific embodiment
In the following embodiments of the present invention, DNA content measurement is detected according to PicoGreen fluorescent dye determination.
Embodiment 1: the removal of peanut oil aflatoxin.
(1) choosing commercially available calf thymus DNA is the present embodiment reagent, and with glycine-hydroxide of 50mM pH value 9.00 Sodium buffer sufficiently dissolves calf thymus DNA, and the calf thymus DNA buffer solution that preparation concentration is 1% is spare.
(2) 50 milliliters of peanut oil are weighed, detect wherein aflatoxin B before handling1Content is 35 micro- gs/kg.
(3) by peanut oil to be processed addition can be with flask of the regulating and controlling temperature with magnetic agitation among, it is yellow bent according to 300 micrograms Mould toxin B1The DNA buffer solution of ratio addition step (1) preparation of/1 gram of DNA, using glycine-sodium hydrate buffer solution as Water lotion is added in peanut oil, makes the 5-10% of buffer total amount peanut oil total weight in peanut oil.
(4) after mixing well, start heating device and magnetic stirring apparatus, be sufficiently stirred, temperature maintains 65 DEG C, persistently stirs It mixes 60 minutes.
(5) 60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains grease.
(6) above-mentioned grease uses pure water washing again, and water additive amount accounts for the 10% of oil mass, is sufficiently mixed, and incorporation time maintains 30 Minute, temperature maintains 50 DEG C.60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains purification grease.This mistake Journey is repeated 1 times.
(7) detection purification grease aflatoxin content situation of change, the results are shown in Table 1.
Embodiment 2: the removal of corn oil aflatoxin.
(1) choosing commercially available calf thymus DNA is the present embodiment reagent, and with glycine-hydroxide of 50mM pH value 9.00 Sodium buffer sufficiently dissolves calf thymus DNA, and the calf thymus DNA buffer solution that preparation concentration is 1% is spare.
(2) 50 milliliters of corn oils are weighed, detect wherein aflatoxin B before handling1Content is 26 micro- gs/kg.
(3) by corn oil to be processed addition can be with flask of the regulating and controlling temperature with magnetic agitation among, it is yellow bent according to 300 micrograms Mould toxin B1The DNA buffer solution of ratio addition step (1) preparation of/1 gram of DNA, using glycine-sodium hydrate buffer solution as Water lotion is added in corn oil, makes the 5-10% of buffer total amount corn oil total weight in corn oil.
(4) after mixing well, start heating device and magnetic stirring apparatus, be sufficiently stirred, temperature maintains 65 DEG C, persistently stirs It mixes 60 minutes.
(5) 60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains grease.
(6) above-mentioned grease uses pure water washing again, and water additive amount accounts for the 10% of oil mass, is sufficiently mixed, and incorporation time maintains 30 Minute, temperature maintains 50 DEG C.60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains purification grease.This mistake Journey is repeated 1 times.
(7) detection purification grease aflatoxin content situation of change, the results are shown in Table 1.
Embodiment 3: the removal of peanut oil aflatoxin.
(1) choosing commercially available salmon sperm dna is the present embodiment reagent, and with sodium carbonate-bicarbonate of 100mM pH value 9.50 Sodium buffer sufficiently dissolves salmon sperm dna, and the salmon sperm dna buffer solution that preparation concentration is 1% is spare.
(2) 100 milliliters of peanut oil are weighed, detect wherein aflatoxin B before handling1Content is 45 micro- gs/kg.
(3) by peanut oil to be processed addition can be with flask of the regulating and controlling temperature with magnetic agitation among, it is yellow bent according to 300 micrograms Mould toxin B1The DNA buffer solution of ratio addition step (1) preparation of/1 gram of DNA, using sodium carbonate-bicarbonate buffer as Water lotion is added in peanut oil, makes the 5-10% of buffer total amount peanut oil total weight in peanut oil.
(4) after mixing well, start heating device and magnetic stirring apparatus, be sufficiently stirred, temperature maintains 65 DEG C, persistently stirs It mixes 60 minutes.
(5) 60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains grease.
(6) above-mentioned grease uses pure water washing again, and water additive amount accounts for the 10% of oil mass, is sufficiently mixed, and incorporation time maintains 30 Minute, temperature maintains 50 DEG C.60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains purification grease.This mistake Journey is repeated 1 times.
(7) detection purification grease aflatoxin content situation of change, the results are shown in Table 1.
Embodiment 4: the removal of corn oil aflatoxin.
(1) choosing commercially available salmon sperm dna is the present embodiment reagent, and with sodium carbonate-bicarbonate of 100mM pH value 9.50 Sodium buffer sufficiently dissolves salmon sperm dna, and the salmon sperm dna buffer solution that preparation concentration is 1% is spare.
(2) 100 milliliters of corn oils are weighed, detect wherein aflatoxin B before handling1Content is 28 micro- gs/kg.
(3) by corn oil to be processed addition can be with flask of the regulating and controlling temperature with magnetic agitation among, it is yellow bent according to 300 micrograms Mould toxin B1The DNA buffer solution of ratio addition step (1) preparation of/1 gram of DNA, using sodium carbonate-bicarbonate buffer as Water lotion is added in corn oil, makes the 5-10% of buffer total amount corn oil total weight in corn oil.
(4) after mixing well, start heating device and magnetic stirring apparatus, be sufficiently stirred, temperature maintains 65 DEG C, persistently stirs It mixes 60 minutes.
(5) 60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains grease.
(6) above-mentioned grease uses pure water washing again, and water additive amount accounts for the 10% of oil mass, is sufficiently mixed, and incorporation time maintains 30 Minute, temperature maintains 50 DEG C.60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains purification grease.This mistake Journey is repeated 1 times.
(7) detection purification grease aflatoxin content situation of change, the results are shown in Table 1.
Embodiment 5: the removal of peanut oil aflatoxin.
(1) choosing cerevisiae dna is the present embodiment reagent, and with the sodium carbonate-bicarbonate of 100mM pH value 9.50 buffering Liquid sufficiently dissolves cerevisiae dna, and the cerevisiae dna buffer solution that preparation concentration is 1% is spare.
(2) 100 milliliters of peanut oil are weighed, detect wherein aflatoxin B before handling1Content is 45 micro- gs/kg.
(3) by peanut oil to be processed addition can be with flask of the regulating and controlling temperature with magnetic agitation among, it is yellow bent according to 300 micrograms Mould toxin B1The DNA buffer solution of ratio addition step (1) preparation of/1 gram of DNA, using sodium carbonate-bicarbonate buffer as Water lotion is added in peanut oil, makes the 5-10% of buffer total amount peanut oil total weight in peanut oil.
(4) after mixing well, start heating device and magnetic stirring apparatus, be sufficiently stirred, temperature maintains 65 DEG C, persistently stirs It mixes 60 minutes.
(5) 60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains grease.
(6) above-mentioned grease uses pure water washing again, and water additive amount accounts for the 10% of oil mass, is sufficiently mixed, and incorporation time maintains 30 Minute, temperature maintains 50 DEG C.60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains purification grease.This mistake Journey is repeated 1 times.
(7) detection purification grease aflatoxin content situation of change, the results are shown in Table 1.
Embodiment 6: the removal of corn oil aflatoxin.
(1) choosing cerevisiae dna is the present embodiment reagent, and sufficiently dissolves ferment with the phosphate buffer of 100mM pH value 8.0 Female DNA, the cerevisiae dna buffer solution that preparation concentration is 1% are spare.
(2) 100 milliliters of corn oils are weighed, detect wherein aflatoxin B before handling1Content is 28 micro- gs/kg.
(3) by corn oil to be processed addition can be with flask of the regulating and controlling temperature with magnetic agitation among, it is yellow bent according to 300 micrograms Mould toxin B1The DNA buffer solution of ratio addition step (1) preparation of/1 gram of DNA, is added phosphate buffer as water lotion Into corn oil, make the 5-10% of buffer total amount corn oil total weight in corn oil.
(4) after mixing well, start heating device and magnetic stirring apparatus, be sufficiently stirred, temperature maintains 65 DEG C, persistently stirs It mixes 60 minutes.
(5) 60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains grease.
(6) above-mentioned grease uses pure water washing again, and water additive amount accounts for the 10% of oil mass, is sufficiently mixed, and incorporation time maintains 30 Minute, temperature maintains 50 DEG C.60 minutes are stood at room temperature, then through the grease that is centrifuged at a high speed, obtains purification grease.This mistake Journey is repeated 1 times.
(7) detection purification grease aflatoxin content situation of change, the results are shown in Table 1.
Aflatoxin content changes list to the different greases of table 1 before and after the processing

Claims (4)

1. a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction, it is characterised in that: this method is to fill Divide and utilize hydrophilic large biological molecule DNA, enabling DNA with the aflatoxin in grease, covalently aspergillus flavus poison is stablized in chimeric formation Element-DNA addition product, then wherein DNA is removed by water-oil separating technique and removes remaining aflatoxin in grease.
2. it is according to claim 1 a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction, It is characterized in that: the washing process refined by traditional edible oil and fat, in traditional washing process link or traditional washing process link Later, directly DNA solution is added among the grease by aflatoxin contamination according to a certain percentage, is sufficiently mixed, and led to Raising temperature, adjustment ionic strength and adjustment pH value are crossed, the aflatoxin intercalation of DNA is promoted, aflatoxin and DNA is promoted to tie Synthesize stable aflatoxin-DNA addition product, then by water-oil separating technique remove wherein DNA remove it is wherein remaining Aflatoxin.
3. it is according to claim 2 a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction, It is characterized in that: the following steps are included:
(1) DNA is dissolved in the buffer being made of phosphate, glycine, sodium hydroxide, sodium carbonate or sodium bicarbonate, is obtained To DNA buffer solution, in 7-10 range, ionic strength is controlled in 50mM-200mM range for the pH value control of DNA buffer solution, DNA concentration is controlled in 0.5-5% range；
(2) aflatoxin content detection is carried out in advance to aflatoxin contamination oil sample；
(3) ratio of -800 microgram aflatoxin of 100 microgram is combined to calculate according to 1 gram of DNA insertion, in traditional washing process ring After section or traditional washing process link, DNA buffer solution prepared by step (1) is added among grease, is added into grease Add with step (1) identical buffer as water lotion, makes the 5-10% of buffer total amount grease total weight in grease；
(4) it is sufficiently mixed, incorporation time maintains -60 minutes 30 minutes, and temperature maintains 50 DEG C -70 DEG C；
(5) it after handling, is allowed to stand at room temperature for 30 minutes -120 minutes, then through oil water separator or the oil that is centrifuged at a high speed Water obtains grease；
(6) grease obtained again through pure water washing 1-2 times, washing water additive amount accounts for the 5%-10% of oil quality, is sufficiently mixed, and mixes Time maintenance -60 minutes 30 minutes is closed, temperature maintains 50 DEG C -70 DEG C, is allowed to stand at room temperature for 30 minutes -120 minutes, then passes through Oil water separator or the grease that is centrifuged at a high speed, obtain purification grease.
4. according to claim 1-3 a kind of based on disappearing with the capture of the grease aflatoxin of DNA packing interaction Except method, it is characterised in that: the DNA is calf thymus DNA, herring sperm dna, salmon sperm dna, cerevisiae dna or cauliflower DNA。
CN201811449992.0A 2018-11-30 2018-11-30 It is a kind of based on capturing removing method with the grease aflatoxin of DNA packing interaction Pending CN109504525A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120225494A1 (en) * 2009-08-21 2012-09-06 Linda Chryseis Le Dna ligands for aflatoxin and zearalenone

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120225494A1 (en) * 2009-08-21 2012-09-06 Linda Chryseis Le Dna ligands for aflatoxin and zearalenone

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Application publication date: 20190322