CN109498837B - Preparation method of sustained-release fiber membrane for releasing growth factors in synergistic gradient by combining coaxial electrostatic spinning and layer-by-layer self-assembly - Google Patents
Preparation method of sustained-release fiber membrane for releasing growth factors in synergistic gradient by combining coaxial electrostatic spinning and layer-by-layer self-assembly Download PDFInfo
- Publication number
- CN109498837B CN109498837B CN201811290535.1A CN201811290535A CN109498837B CN 109498837 B CN109498837 B CN 109498837B CN 201811290535 A CN201811290535 A CN 201811290535A CN 109498837 B CN109498837 B CN 109498837B
- Authority
- CN
- China
- Prior art keywords
- layer
- electrostatic spinning
- preparation
- growth factors
- assembly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/28—Formation of filaments, threads, or the like while mixing different spinning solutions or melts during the spinning operation; Spinnerette packs therefor
- D01D5/30—Conjugate filaments; Spinnerette packs therefor
- D01D5/34—Core-skin structure; Spinnerette packs therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/45—Mixtures of two or more drugs, e.g. synergistic mixtures
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/606—Coatings
- A61L2300/608—Coatings having two or more layers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Epidemiology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- General Health & Medical Sciences (AREA)
- Transplantation (AREA)
- Animal Behavior & Ethology (AREA)
- Mechanical Engineering (AREA)
- Textile Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention provides a method for releasing growth factors by combining coaxial electrostatic spinning and layer-by-layer self-assembly in a synergistic gradient manner, which comprises the following steps: step 1, wrapping one or more growth factors in a core layer solution by using a coaxial electrostatic spinning method; step 2, loading another growth factor or growth factors to the surface of the coaxial nanofiber by using a layer-by-layer self-assembly method; and 3, freeze drying to obtain the coaxial electrostatic spinning/layer-by-layer self-assembled slow-release fiber membrane. The method integrates one growth factor or drug with a core layer solution by utilizing a coaxial electrostatic spinning technology, and then deposits the other growth factor or drug on the surface of the nanofiber membrane by utilizing a layer-by-layer self-assembly technology. The two methods can realize the tight combination of the core layer factor and the loose combination of the surface layer factor, and finally achieve the combined slow release effect of the long-acting release of the core layer and the instant release of the shell layer factor, thereby having very wide application prospect in the fields of wound healing and tissue engineering.
Description
Technical Field
The invention belongs to the technical field of materials, and particularly relates to a preparation method of a sustained-release fiber membrane for releasing growth factors in a synergistic gradient manner by combining coaxial electrostatic spinning and layer-by-layer self-assembly.
Background
The three elements of the tissue engineering are as follows: scaffold material, seed cells and growth factors. The growth factors with different physiological functions can promote the proliferation and differentiation of seed cells and improve the integration efficiency of the scaffold material and autologous tissues. In addition, the various growth factors of the body do not play their own functions singly, but act synergistically to promote tissue repair.
However, previous studies have simply incorporated growth factors into scaffolds and do not take into account the need for synergistic release of growth factors. Thus, the synergistic release of different growth factors while simultaneously applying them is an indispensable step in the successful repair of tissue defects.
The electrostatic spinning technology is an emerging method for preparing nano-to submicron-scale fiber scaffolds. Conventional electrospinning apparatus include spraying, receiving and high voltage power supply means. Under the action of a high-voltage electric field, spinning solution is sprayed out of a spinneret orifice and finally a fiber film is formed. The principle of coaxial electrospinning is the same as that of the traditional electrospinning technology, but some improvements are made on the spinning equipment: and respectively injecting the core layer solution and the shell layer solution into two different tubular injectors according to specific requirements, and applying electric fields with different voltages to enable the core layer solution and the shell layer solution to form coaxial stratified flow. Under the action of a high-voltage electric field, the shell-core double-layer solution is subjected to high-frequency stretching, deformation and curing to form the coaxial nanofiber. The layer-by-layer self-assembly technology is a technology which utilizes chemical bonds, coordination bonds, hydrogen bonds and electrostatic attraction among substances to drive a target compound to spontaneously and alternately deposit layer by layer on a substrate to form one or more layers of functional thin films.
The controlled release of the drug and the bioactive substance can be realized by utilizing the coaxial electrostatic spinning and the layer-by-layer self-assembly technology. Growth factors or drugs can be integrated with the core layer solution using coaxial electrospinning techniques. After the nano-fiber is formed, the slow-release substance is fixed in the fiber, and the shell layer can play the roles of protecting the core layer solution and delaying the release time. The growth factors can be deposited on the surface of the substrate by using the layer-by-layer self-assembly technology.
Chinese patent "a VEGF-loaded slow-release system using mesenchymal stem cells as carriers and through layer-by-layer self-assembly and a preparation method thereof" (publication No. CN107693778A) discloses a method for slowly releasing VEGF through a layer-by-layer self-assembly technology. The patent loads Vascular Endothelial Growth Factor (VEGF) on a gelatin and alginate drug-loaded sustained-release system by a layer-by-layer self-assembly technology. The slow release system prepared by the patent can enable the MSCs encapsulated with VEGF to migrate to the myocardial infarction part and slowly release VEGF, thereby achieving the effects of promoting the regeneration of blood vessels and perfusing the infarcted area. The preparation process of the sustained-release system in the patent does not relate to a coaxial electrostatic spinning technology, only loads a growth factor VEGF singly, and does not relate to the synergistic release of the growth factor.
Chinese patent 'preparation and application of nerve growth factor slow release nano-carrier' (publication No. CN104027793A) discloses a preparation method of a nerve growth factor slow release nano-carrier. The method comprises the following specific steps: (1) preparation of external, internal and oil phases: the external water phase is 0.7% polyvinyl alcohol solution; the internal water phase is 5% polyethylene glycol; the oil phase is 3: 1 of dichloromethane and acetone; (2) preparing a polylactic acid-glycolic acid microsphere slow-release system loaded with nerve growth factors. Although the patent is also a prepared core-shell structure slow-release system, microspheres are used as carriers instead of electrospinning nano fibers. In addition, the microsphere sustained-release system prepared by the patent only loads one growth factor, and cannot meet the requirement of the growth factor for the synergistic release.
Disclosure of Invention
Aiming at the problems in the prior art, the technical scheme adopted by the invention for solving the problems in the prior art is as follows:
a preparation method of a sustained-release fiber membrane for releasing growth factors by combining coaxial electrostatic spinning and layer-by-layer self-assembly in a synergistic gradient manner is characterized by comprising the following steps:
step one, wrapping one or more growth factors in a core layer solution by using a coaxial electrostatic spinning method;
loading another growth factor or growth factors to the surface of the coaxial nanofiber by using a layer-by-layer self-assembly method;
and step three, obtaining the coaxial electrostatic spinning/layer-by-layer self-assembly slow-release fiber membrane after freeze drying.
In the first step, the core layer solution comprises the following components: one or more natural or synthetic polymer materials.
In the first step, the growth factors of the core layer are as follows: one or more growth factors or biologically active substances. For example, bioactive substances such as bone morphogenetic protein 2, bone morphogenetic protein 4, platelet-rich plasma, etc. can be selected for the sustained release system to have osteogenic ability; if the sustained release system is to be made angiopoietic, vascular endothelial growth factor, fibroblast growth factor, etc. may be selected.
In the first step, the core layer material is chitosan, collagen, polyethylene glycol, fibroin, silk fibroin, polylactic acid, polyvinyl alcohol and the like, and the available solvent comprises one or more of water-based solvents such as water, ethanol and the like, and core layer material solutions with different concentrations (5% -20%) are prepared.
In the first step, a shell layer wrapping the core layer by using a coaxial electrostatic spinning method is made of a high polymer material, specifically polycaprolactone, silk fibroin, polylactic acid, chitosan, polyacrylonitrile, polyaniline, polystyrene, polyvinylpyrrolidone, polyethylene oxide and the like. The selected solvent comprises one or more of hexafluoroisopropanol, methanol, amyl alcohol, trichloromethane, dichloromethane, tetrahydrofuran, N dimethylformamide, N-dimethylacetamide, toluene, chlorobenzene, phosphoric acid, formic acid and trifluoroacetic acid, and spinning solutions with different concentrations (8% -20%) are prepared.
In the first step, if a shell layer of the core layer is wrapped by using a coaxial electrostatic spinning method, water-soluble high polymer materials are selected, and specifically polyvinyl alcohol, silk fibroin, polyethylene oxide and the like are adopted.
In the second step, the number of self-assembly times of the layers is 1 or more.
In the second step, one or more growth factors or bioactive substances loaded on the coaxial nanofiber membrane by a layer-by-layer self-assembly method are adopted according to specific needs. For example, bioactive substances such as bone morphogenetic protein 2, bone morphogenetic protein 4, platelet-rich plasma, etc. can be selected for the sustained release system to have osteogenic ability; if the sustained release system is to be made angiopoietic, vascular endothelial growth factor, fibroblast growth factor, etc. may be selected.
In the first step, when a coaxial electrostatic spinning method is used, the set voltage is 16-20 kV, the core layer solution is propelled at the speed of 0.5-0.9 mL/h, the shell layer solution is propelled at the speed of 1.6-2.4 mL/h into the spinning solution, the distance between the spinning solution and a receiver is set to be 12-15 cm, the spinning temperature is 25 ℃, and the relative humidity is 40% -50%.
And in the third step, the coaxial electrostatic spinning/layer-by-layer self-assembly slow-release fiber membrane is freeze-dried and stored at a low temperature for later use.
The invention has the following advantages:
the invention combines the coaxial electrostatic spinning and the layer-by-layer self-assembly technology to prepare a slow-release system which can synergistically release different bioactive substances, can load different bioactive substances into the core layer and the surface of the shell layer of the coaxial spinning nanofiber, and can quickly and slowly release the bioactive substances according to specific needs. Thus, the effect of coordinated slow release of various bioactive substances can be achieved. The operation method is simple and convenient, and the effect is obvious, so the synergistic release slow-release system developed by the invention has wide application prospect in the fields of tissue engineering and biomedicine.
Drawings
FIG. 1 is a scanning electron micrograph of a polycaprolactone/polyvinyl alcohol coaxial electrospun membrane prepared in a first example of the invention;
FIG. 2 is a scanning electron microscope image of a polycaprolactone/polyvinyl alcohol coaxial electrostatic spinning film after layer-by-layer self-assembly in accordance with one embodiment of the present invention;
FIG. 3 is a confocal microscope of polycaprolactone/polyethylene oxide prepared in the second embodiment of the present invention, showing the double-layer structure of the fiber;
FIG. 4 is a Masson staining chart of a mouse skull defect specimen covered by a polycaprolactone/polyvinyl alcohol coaxial electrospun nanofiber membrane in accordance with one embodiment of the present invention;
FIG. 5 is a Masson staining of a mouse skull defect specimen covered by an autologous skull.
Detailed Description
The technical scheme of the invention is further described in detail by the following embodiments and the accompanying drawings:
example one
Step 1, preparing polycaprolactone/polyvinyl alcohol coaxial electrostatic spinning nanofiber membrane loaded with BMP 2:
dissolving 1g of polycaprolactone in 9g of hexafluoroisopropanol solvent to obtain a shell layer composite solution with the mass fraction of 10%; 0.8g of polyvinyl alcohol was dissolved in 9.2g of deionized water to prepare a core layer composite solution with a mass fraction of 8% and BMP2 growth factor was added. Then, preparing a polycaprolactone/polyvinyl alcohol coaxial nanofiber membrane by adopting a coaxial electrostatic spinning technology, and then, carrying out vacuum drying on the obtained polycaprolactone/polyvinyl alcohol composite fiber membrane in a vacuum drying oven for 24 hours to fully volatilize the solvent; the electrostatic spinning voltage is 18kV, the shell layer solution advances the spinning solution at the speed of 1.8mL/h, the core layer solution advances at the speed of 0.8mL/h, the distance between the core layer solution and the receiver is set to be 15cm, the spinning temperature is 25 ℃, and the relative humidity is 40%;
vacuum drying the fiber membrane, and sterilizing for 24h by ultraviolet irradiation.
Step 2, loading CTGF to the surface of the nanofiber membrane by using a layer-by-layer self-assembly method:
and (3) soaking the polycaprolactone/polyvinyl alcohol coaxial spinning nanofiber membrane obtained in the step into a 1% chitosan solution for 3 minutes, taking out the polycaprolactone/polyvinyl alcohol coaxial spinning nanofiber membrane, and placing the polycaprolactone/polyvinyl alcohol coaxial spinning nanofiber membrane into a CTGF growth factor solution for 3 minutes. Repeating the process for 20 times to obtain the coaxial nano spinning film with the CTGF growth factors loaded on the surface.
Example two
Step 1, preparing a polycaprolactone/polyethylene oxide coaxial electrostatic spinning nanofiber membrane loaded with BMP4 inside:
dissolving 1g of polycaprolactone in 9g of hexafluoroisopropanol solvent to obtain a shell layer composite solution with the mass fraction of 10%; 1g of polyethylene oxide was dissolved in 9g of deionized water to prepare a core layer composite solution with a mass fraction of 10% and BMP4 growth factor was added. Then preparing a polycaprolactone/polyethylene oxide coaxial nanofiber membrane by adopting a coaxial electrostatic spinning technology, and then carrying out vacuum drying on the obtained polycaprolactone/polyethylene oxide composite fiber membrane in a vacuum drying oven for 24 hours to fully volatilize the solvent; the electrostatic spinning voltage is 18kV, the shell layer solution advances the spinning solution at the speed of 1.8mL/h, the core layer solution advances at the speed of 0.8mL/h, the distance between the core layer solution and the receiver is set to be 15cm, the spinning temperature is 25 ℃, and the relative humidity is 40%;
vacuum drying the fiber membrane, and sterilizing for 24h by ultraviolet irradiation.
Step 2, loading VEGF to the surface of the nanofiber membrane by using a layer-by-layer self-assembly method:
and (3) soaking the polycaprolactone/polyvinyl alcohol coaxial spinning nanofiber membrane obtained in the step into a 1% chitosan solution for 3 minutes, taking out the polycaprolactone/polyvinyl alcohol coaxial spinning nanofiber membrane, and placing the polycaprolactone/polyvinyl alcohol coaxial spinning nanofiber membrane into a VEGF growth factor solution for 3 minutes. Repeating the process for 20 times to obtain the coaxial nano spinning membrane loaded with the VEGF growth factor on the surface.
As shown in fig. 4 and 5, after covering the skull defect with the polycaprolactone/polyvinyl alcohol coaxial electrospun nanofiber membrane loaded with BMP2 and CTGF for 8 weeks, it was seen that the bone defect covered with the polycaprolactone/polyvinyl alcohol coaxial electrospun nanofiber membrane healed well, and the bony healing was substantially completed. Bone defects without a fibrous membrane covering are not completely healed, and more fibrous tissues are formed at the bone defects. Polycaprolactone/polyvinyl alcohol loaded with BMP2 and CTGF was shown to promote bone healing and new bone formation.
The protective scope of the present invention is not limited to the above-described embodiments, and it is apparent that various modifications and variations can be made to the present invention by those skilled in the art without departing from the scope and spirit of the present invention. It is intended that the present invention cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents.
Claims (9)
1. A preparation method of a sustained-release fiber membrane for releasing growth factors by combining coaxial electrostatic spinning and layer-by-layer self-assembly in a synergistic gradient manner is characterized by comprising the following steps:
step one, wrapping one or more growth factors in a core layer solution by using a coaxial electrostatic spinning method;
loading another growth factor or growth factors to the surface of the coaxial nanofiber by using a layer-by-layer self-assembly method;
step three, obtaining a coaxial electrostatic spinning/layer-by-layer self-assembly slow-release fiber membrane after freeze drying;
in the first step, the core layer material is chitosan, collagen, polyethylene glycol, fibroin, silk fibroin, polylactic acid or polyvinyl alcohol, and the solvent of the core layer material solution comprises one or more of water and ethanol so as to prepare core layer material solutions with different concentrations;
in the first step, a shell layer for wrapping the core layer by using a coaxial electrostatic spinning method is made of a high polymer material, specifically polycaprolactone, silk fibroin, polylactic acid, chitosan, polyvinylpyrrolidone or polyethylene oxide, and a solvent selected from the shell layer material comprises one or more of hexafluoroisopropanol, methanol, amyl alcohol, trichloromethane, dichloromethane, tetrahydrofuran, N-dimethylformamide, N-dimethylacetamide, toluene, chlorobenzene, phosphoric acid, formic acid and trifluoroacetic acid to prepare spinning solutions with different concentrations.
2. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: in the first step, the growth factors of the core layer are as follows: one or more growth factors or biologically active substances.
3. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: the concentration of the core layer material solution with different concentrations is 5% -20%.
4. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: the concentration of the spinning solution with different concentrations is 8-20%.
5. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: in the first step, a shell layer wrapping the core layer by using a coaxial electrostatic spinning method is made of a water-soluble high polymer material, specifically silk fibroin or polyethylene oxide.
6. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: in the second step, the number of self-assembly times of the layers is 1 or more.
7. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: in the second step, one or more growth factors loaded on the coaxial nanofiber membrane by a layer-by-layer self-assembly method are adopted according to specific needs.
8. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: in the first step, when a coaxial electrostatic spinning method is used, the set voltage is 16-20 kV, the core layer solution is propelled at the speed of 0.5-0.9 mL/h, the shell layer solution is propelled at the speed of 1.6-2.4 mL/h, the distance between the receiver and the spinning solution is set to be 12-15 cm, the spinning temperature is 25 ℃, and the relative humidity is 40% -50%.
9. The preparation method of the sustained-release fiber membrane for releasing the growth factors by the combined coaxial electrostatic spinning and layer-by-layer self-assembly cooperative gradient as claimed in claim 1, wherein the preparation method comprises the following steps: and in the third step, the coaxial electrostatic spinning/layer-by-layer self-assembly slow-release fiber membrane is freeze-dried and stored at a low temperature for later use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811290535.1A CN109498837B (en) | 2018-10-31 | 2018-10-31 | Preparation method of sustained-release fiber membrane for releasing growth factors in synergistic gradient by combining coaxial electrostatic spinning and layer-by-layer self-assembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811290535.1A CN109498837B (en) | 2018-10-31 | 2018-10-31 | Preparation method of sustained-release fiber membrane for releasing growth factors in synergistic gradient by combining coaxial electrostatic spinning and layer-by-layer self-assembly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109498837A CN109498837A (en) | 2019-03-22 |
| CN109498837B true CN109498837B (en) | 2021-08-03 |
Family
ID=65747311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811290535.1A Active CN109498837B (en) | 2018-10-31 | 2018-10-31 | Preparation method of sustained-release fiber membrane for releasing growth factors in synergistic gradient by combining coaxial electrostatic spinning and layer-by-layer self-assembly |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109498837B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112210889A (en) * | 2020-09-17 | 2021-01-12 | 浙江理工大学 | Preparation method of ordered shell-core type high-conductivity nano material |
| CN112301542A (en) * | 2020-10-20 | 2021-02-02 | 福建农林大学 | Core-shell type composite nanofiber membrane and preparation method and application thereof |
| CN112779644B (en) * | 2020-12-24 | 2022-06-07 | 陕西元丰纺织技术研究有限公司 | Temperature-adjusting antibacterial mosquito-proof yarn and fabric and preparation method thereof |
| CN115025295B (en) * | 2022-05-27 | 2023-02-28 | 上海大学 | Intravascular stent tectorial membrane with coagulation promoting and long-term antibacterial effects and preparation method thereof |
| CN115737889A (en) * | 2022-11-08 | 2023-03-07 | 湖北大学 | Electrostatic spinning nanofiber dressing for skin wound repair and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101509153A (en) * | 2009-03-23 | 2009-08-19 | 东华大学 | Method for producing shell-core structure medicament nano-fibre with coaxial electrostatic spinning technology |
| CN102000360A (en) * | 2010-10-26 | 2011-04-06 | 华南理工大学 | Metal implant with bioactive surface modification and preparation method thereof |
| CN103893819A (en) * | 2014-03-20 | 2014-07-02 | 北京大学第三医院 | Coaxial electrostatic spinning fibrous scaffold and preparation method thereof |
| WO2015054677A1 (en) * | 2013-10-12 | 2015-04-16 | Innovative Surface Technologies, Inc. | Tissue scaffolds for electrically excitable cells |
| CN107096066A (en) * | 2017-04-24 | 2017-08-29 | 东华大学 | A kind of application of the double drug-loading fibre supports of the coaxial PLA caprolactone PLCL/ gelatin of pH sensitiveness |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008157372A2 (en) * | 2007-06-14 | 2008-12-24 | Massachusetts Institute Of Technology | Self assembled films for protein and drug delivery applications |
-
2018
- 2018-10-31 CN CN201811290535.1A patent/CN109498837B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101509153A (en) * | 2009-03-23 | 2009-08-19 | 东华大学 | Method for producing shell-core structure medicament nano-fibre with coaxial electrostatic spinning technology |
| CN102000360A (en) * | 2010-10-26 | 2011-04-06 | 华南理工大学 | Metal implant with bioactive surface modification and preparation method thereof |
| WO2015054677A1 (en) * | 2013-10-12 | 2015-04-16 | Innovative Surface Technologies, Inc. | Tissue scaffolds for electrically excitable cells |
| CN103893819A (en) * | 2014-03-20 | 2014-07-02 | 北京大学第三医院 | Coaxial electrostatic spinning fibrous scaffold and preparation method thereof |
| CN107096066A (en) * | 2017-04-24 | 2017-08-29 | 东华大学 | A kind of application of the double drug-loading fibre supports of the coaxial PLA caprolactone PLCL/ gelatin of pH sensitiveness |
Non-Patent Citations (4)
| Title |
|---|
| 《Coaxial electrospinning multicomponent functional controlled-release vascular graft: Optimization of graft properties》;Anlin Yin等;《Colloids and Surfaces B: Biointerfaces》;20170127;第152卷;432-439 * |
| 《Electrospun polymeric nanofibres as wound dressings: A review》;Sónia P. Miguel等;《Colloids and Surfaces B: Biointerfaces》;20180505;第169卷;60-71 * |
| 《Incorporating platelet-rich plasma into coaxial electrospun nanofibers for bone tissue engineering》;Gu Cheng等;《International Journal of Pharmaceutics》;20180607;第547卷(第1-2期);656-666 * |
| 《Regulating the gaps between folds on the surface of silk fibroin membranes via LBL deposition for improving their biomedical properties》;Guomin Wu等;《Colloids and Surfaces B: Biointerfaces》;20170301;第154卷;228-238 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109498837A (en) | 2019-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109498837B (en) | Preparation method of sustained-release fiber membrane for releasing growth factors in synergistic gradient by combining coaxial electrostatic spinning and layer-by-layer self-assembly | |
| Liu et al. | Biomimetic organic-inorganic hybrid hydrogel electrospinning periosteum for accelerating bone regeneration | |
| US11946164B2 (en) | Nanofiber structures and methods of use thereof | |
| Wu et al. | Effect of scaffold morphology and cell co-culture on tenogenic differentiation of HADMSC on centrifugal melt electrospun poly (L‑lactic acid) fibrous meshes | |
| Li et al. | Functionalized silk fibroin dressing with topical bioactive insulin release for accelerated chronic wound healing | |
| Shin et al. | Contractile cardiac grafts using a novel nanofibrous mesh | |
| Norouzi et al. | PLGA/gelatin hybrid nanofibrous scaffolds encapsulating EGF for skin regeneration | |
| EP3351376B1 (en) | Silk biomaterials and methods of use thereof | |
| WO2008069760A1 (en) | Three-dimensional porous hybrid scaffold and manufacture thereof | |
| CN114108177B (en) | Artificial skin material capable of triggering growth factor stage release by photo-thermal, preparation method and application thereof | |
| CN114099759B (en) | Fiber wound repair bracket loaded with phase change material particles and preparation method and application thereof | |
| CN1733311A (en) | The preparation method of the nanofiber of a kind of packaging medicine or somatomedin | |
| US20210008253A1 (en) | Elongate scaffold comprising inner and outer portion | |
| CN105525385B (en) | A kind of multi-layer core-shell nano fiber scaffold and its method with melanocyte building tissue engineering material | |
| Yao et al. | Dual‐Drug‐Loaded Silk Fibroin/PLGA Scaffolds for Potential Bone Regeneration Applications | |
| Hussain et al. | Biomedical applications of nanofiber scaffolds in tissue engineering | |
| Liu et al. | Progress in electrospun fibers for manipulating cell behaviors | |
| Shehata et al. | Spider silk fibers: synthesis, characterization, and related biomedical applications | |
| Wang et al. | Bladder muscle regeneration enhanced by sustainable delivery of heparin from bilayer scaffolds carrying stem cells in a rat bladder partial cystectomy model | |
| CN109602953A (en) | A kind of New-type long-acting sustained release VEGF and bFGF degradable biological nanometer diaphragm and preparation method thereof | |
| Saeed et al. | Surface morphology and biochemical characteristics of electrospun cellulose nanofibril reinforced PLA/PBS hollow scaffold for tissue engineering | |
| Li et al. | Optimization of the electrospinning process for core–shell fiber preparation | |
| Zhou et al. | Manipulating electrostatic field to control the distribution of bioactive proteins or polymeric microparticles on planar surfaces for guiding cell migration | |
| Li et al. | Fabrication and characterization of three-dimensional poly (ε-caprolactone) bilayer scaffolds for skin regeneration | |
| CN114808276B (en) | Preparation method of nerve conduit with three-layer structure and nerve conduit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |