CN109498576A - A kind of preparation method for the composite phospholipid liposome that low-ester pectin is stable - Google Patents
A kind of preparation method for the composite phospholipid liposome that low-ester pectin is stable Download PDFInfo
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Abstract
A kind of preparation method for the composite phospholipid liposome that low-ester pectin is stable, it carries out in accordance with the following steps: (1) weighing soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, vitamin C mixing, it is dissolved in dehydrated alcohol, water bath sonicator obtains mixture up to being completely dissolved；(2) mixture is depressurized to rotary evaporation under 40-50 DEG C of water bath condition and removes ethyl alcohol, until forming one layer of uniform liposome membrane in revolving bottle wall；(3) compound for phosphate buffer and mass ratio being added into revolving bottle as the EGCG of 1:2:1:1, vitamin E, albumin and resveratrol obtains the thick suspension of liposome wherein rotation is hydrated 30-60min under 40-50 DEG C of water bath condition；(4) the low-ester pectin aqueous solution for configuring 0.1-0.6g/100mL, the thick suspension of liposome is added drop-wise in pectin solution, is mixed according to volume 1:1, most obtains the stable composite phospholipid liposome of low-ester pectin through ice-bath ultrasonic afterwards.Preparation method of the present invention can effectively improve the sustained release performance and inoxidizability of liposome.
Description
(1) technical field:
The present invention relates to a kind of preparation methods of composite phospholipid liposome that low-ester pectin is stable.
(2) background technique:
Liposome is the conventional medicine-releasing system of one kind in pharmacy, may be used as the form of administration of insoluble drug, improves
Its bioavilability.But drug is also easy to produce leakage etc. and asks when there are encapsulation rates low, unstable, the long-term storage of common unilamellar liposome
Topic, causes Liposomal formulation research and development of products to be subject to certain restrictions.Composite phospholipid liposome is as a kind of novel lipide, with tradition
Liposome is compared, and has many advantages, such as that drug does not generate leakage, and the intersection row of composite phospholipid when preferable stability, storage for a long time
Team improves the rigidity of liposome, has preferable application prospect.
Traditional liposomal coats resveratrol, is typically present in the bimolecular of phosphatide, resveratrol can be improved
Load capacity.But this is also likely to the stability and sensibility of influence liposome bilayer membrane.In addition to double-layer of lipoid can be destroyed
Outside, due to the strong coherence between hydrophobic drug and double-layer of lipoid, the hydrophobic drug for including in liposome lipid bilayer can
It can not be released effectively.
Pectin is a kind of soluble dietary fiber of non-starch polysaccharide class, has gel characteristic, has been widely used as gel
Agent, thickener and stabilizer etc..Also, for high-ester pectin, low-ester pectin can shape as long as coexisting with metal ion
At gel.This gel can reversibly become liquid after heating or stirring, can restore former solidifying again after stopping stirring or cooling
It is gluey.Low-ester pectin is added as stabilizer and gelling agent, on the one hand can assign product unique physiological function, improves product
Added value；Product viscosity can be improved in another aspect polysaccharide, optimizes products'texture quality.
China Patent No. is that CN201710396375.8 " a kind of EGCG micro pill capsule and preparation method thereof " discloses one kind
The microcapsules production method of EGCG, traditional microcapsules technology will cause the inactivation of active constituent, dropped significantly using spray drying
Its low nutritive value.China Patent No. is " a kind of resveratrol lipidosome gel and its preparation side CN201510072466.7
Method " a kind of preparation method of resveratrol gel is disclosed, gel is formed using triethanolamine, triethanolamine has carcinogenicity.
(3) summary of the invention
It is an object of the invention to a kind of preparation methods of composite phospholipid liposome that low-ester pectin is stable, to improve lipid
The sustained release performance and inoxidizability of body.
The technical solution adopted by the invention is as follows:
A kind of preparation method for the composite phospholipid liposome that low-ester pectin is stable carries out in accordance with the following steps:
(1) soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, vitamin C mixing are weighed, is dissolved in dehydrated alcohol, water
Bath ultrasound obtains mixture up to being completely dissolved；Wherein soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, ascorbic
The mass ratio that feeds intake is 8-10:8-10:4-5:1, and the volumetric usage of dehydrated alcohol is calculated as 0.8- with ascorbic quality dosage
1.2L/g；
(2) mixture that step (1) obtains is depressurized under 40-50 DEG C of water bath condition rotary evaporation and removes ethyl alcohol, until
It rotates and forms one layer of uniform liposome membrane in bottle wall；
(3) phosphate buffer and mass ratio that pH=7.4,0.05mol/L are added into revolving bottle are 1:2:1:1's
EGCG, vitamin E, albumin and resveratrol compound, under 40-50 DEG C of water bath condition rotation hydration 30-60min,
The thick suspension of liposome is obtained, wherein the volumetric usage of phosphate buffer is calculated as 4-5L/g with the quality dosage of resveratrol, white
Veratryl alcohol and ascorbic mass ratio are 0.4-0.6:1；
(4) the low-ester pectin aqueous solution for configuring 0.1-0.6g/100mL, the thick suspension of liposome is added drop-wise in pectin solution,
It is mixed according to volume 1:1, most obtains the stable composite phospholipid liposome of low-ester pectin through ice-bath ultrasonic afterwards.
Further, in step (1), the volumetric usage of dehydrated alcohol is preferably 1.0- with meter with ascorbic quality
1.2L/g。
Further, in step (1), water bath sonicator condition are as follows: the ultrasound 3min under the conditions of 300w.
Further, in step (2), vacuum rotary steam evaporation conditions are as follows: evaporation is rotated under the conditions of vacuum degree 0.08MPa
40min。
Further, in step (2), bath temperature is 45 DEG C.
Further, in step (3), the resveratrol and ascorbic mass ratio are 0.5:1.The phosphate-buffered
The volumetric usage of liquid is calculated as 4L/g with the quality dosage of resveratrol.
Further, in step (3), preferably 45 DEG C of bath temperature, the preferred 45min of hydration time is rotated.
Further, in step (4), the concentration of low-ester pectin aqueous solution is 0.2-0.3g/100mL.
The present invention specifically recommends the preparation method to carry out in accordance with the following steps:
(1) soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, vitamin C mixing are weighed, is dissolved in dehydrated alcohol, water
Bath ultrasound obtains mixture up to being completely dissolved；Wherein soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, ascorbic
The mass ratio that feeds intake is 8-10:8-10:4-5:1, and the volumetric usage of dehydrated alcohol is calculated as 1.0- with ascorbic quality dosage
1.2L/g；
(2) mixture for obtaining step (1) depressurizes rotary evaporation under 45 DEG C of water bath conditions and removes ethyl alcohol, until revolving
One layer of uniform liposome membrane is formed in bottle wall；
(3) phosphate buffer and mass ratio that pH=7.4,0.05mol/L are added into revolving bottle are 1:2:1:1's
EGCG, vitamin E, albumin and resveratrol compound, under 45 DEG C of water bath condition rotation hydration 45min, obtain rouge
The thick suspension of plastid, wherein the volumetric usage of phosphate buffer is calculated as 4L/g with the quality dosage of resveratrol, resveratrol with
Ascorbic mass ratio is 0.5:1；
(4) the low-ester pectin aqueous solution for configuring 0.2-0.3g/100mL, the thick suspension of liposome is added drop-wise in pectin solution,
It is mixed according to volume 1:1, most obtains the stable composite phospholipid liposome of low-ester pectin through ice-bath ultrasonic afterwards.
The beneficial effects are mainly reflected as follows:
1, membrane material of the present invention by using composite phospholipid as liposome improves the stability and membrane material of liposome
Rigidity.
The structure of composite phospholipid liposome is a kind of ball-type pharmaceutical carrier of the phospholipid bilayer of similar biofilm structure,
It is during the preparation process that membrane material is (a kind of high using the phosphatide (hydrogenated soya phosphatide, soybean lecithin) of two kinds of different phase transition temperatures
In room temperature, one kind being lower than room temperature), two kinds of phosphatide are spaced, so that composite phospholipid film has rigid structure, make between phospholipid molecule
Arrangement it is even closer orderly, be formed by structure it is stronger and stablize, improve the rigid structure function of liposome.With biography
System liposome is compared, and composite phospholipid can be by intermolecular interaction, and due to foring difference in composite phospholipid liposome
Alpha region, obtained liposome reduces the leakage of drug in vitro, overcomes that traditional liposomal drugloading rate is relatively low etc. to ask
Topic, so that composite phospholipid liposome has more preferable stability and bioavilability.
2, the liposome to be formed is modified by low-ester pectin has stronger slow-release function than traditional unmodified liposome,
Specifically, sustained release cycle stretch-out 5~10 days.
Low-ester pectin is as a kind of adsorptivity polysaccharide, when it is coated on the superficies of phosphatide, forms interfacial film, secondly
Low-ester pectin has lower esterification degree, and the absolute value of zeta current potential is higher, has bigger electrostatic repulsion between particle.Low ester
The ion and thermal stability of liposome can be improved by steric hindrance effect for the polyelectrolyte on pectin Liposomes Liposomes surface, and
And the liposome can more efficiently prevent from the leakage of core material in pipe intestinal digesting.
3, the carrier with albumin as lyophobic dust resveratrol can reside in the hydrophilic core of liposome,
The sustained release of effective control resveratrol and the stability for improving liposome.
Due to the strong coherence between hydrophobic drug and double-layer of lipoid, the stability of liposome bilayer membrane and quick is influenced
Perception.Other than double-layer of lipoid can be destroyed, the hydrophobic drug for including in liposome lipid bilayer possibly can not be released effectively.
Albumin is the natural carrier of hydrophobic molecule, the feature that there is good, invertibity to combine.Resveratrol passes through with albumin
Hydrophobic effect is linked together, and lyophobic dust can be present in water core by carrier of the albumin as lyophobic dust, non-
The resveratrol particle of the disordered state of crystal is in the state that can be bioavailable, the effective sustained release for controlling resveratrol with
And improve the stability of liposome.
(4) Detailed description of the invention
Fig. 1 is the preparation technology flow chart of traditional liposomal；
Fig. 2 is liposome preparation process flow chart of the invention.
(5) content is embodied
With specific embodiment, technical scheme is described further below, but protection scope of the present invention is unlimited
In this:
Raw material sources: low-ester pectin (permanent sharp biological food science and technology), soybean lecithin (Aladdin), hydrogenated soy phosphatidyl choline
(Nanjing Dou Lai Bioisystech Co., Ltd), albumin (Aladdin), EGCG (upper Hiroad standing grain medical sci-tech Development Co., Ltd),
Injection Vitamin B_6 (Aladdin).
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This:
Embodiment 1:
(1) 80mg soybean lecithin, 80mg hydrogenated soy phosphatidyl choline, 40mg cholesterol, 10mg vitamin C are accurately weighed,
Mixing, is dissolved in the dehydrated alcohol of 12mL, and water bath sonicator 3min obtains mixture up to being completely dissolved under the conditions of 300W.
(2) mixture for obtaining step (1) depressurizes (vacuum degree 0.08MPa) rotary evaporation under 45 DEG C of water bath conditions
40min removes ethyl alcohol, until forming one layer of uniform liposome membrane in revolving bottle wall.
(3) into revolving bottle be added 20mL 0.05mol/L phosphate buffer (pH=7.4) and 5mgEGCG,
The compound of 10mg vitamin E, 5mg albumin and 5mg resveratrol, rotation hydration 45min, obtains under 45 DEG C of water bath condition
To the thick suspension of liposome.
(4) the low-ester pectin aqueous solution for configuring the 0.2g/100mL of 20mL, is added drop-wise to pectin solution for the thick suspension of liposome
In, it is mixed according to volume ratio 1:1, is finally placed in ice-bath ultrasonic 5min in probe type ultrasonication instrument (1s/1s, 500W) and obtains
The stable composite phospholipid liposome of low-ester pectin.
Comparative example 1:
(1) 160mg soybean lecithin, 40mg cholesterol, 5mg resveratrol, 10mg vitamin C are accurately weighed, is mixed, it is molten
In the dehydrated alcohol of 12mL, water bath sonicator 3min obtains mixture up to being completely dissolved under the conditions of 300W.
(2) mixture for obtaining step (1) depressurizes (vacuum degree 0.08MPa) rotary evaporation under 45 DEG C of water bath conditions
40min removes ethyl alcohol, until forming one layer of uniform liposome membrane in revolving bottle wall.
(3) into revolving bottle be added 20mL 0.05mol/L phosphate buffer (pH=7.4) and 5mgEGCG and
10mg vitamin E, rotation hydration 45min, obtains the thick suspension of liposome under 45 DEG C of water bath condition.
(4) the thick suspension of liposome is placed in ice-bath ultrasonic 5min in probe type ultrasonication instrument (1s/1s, 500W) can be obtained rouge
Plastid.
Embodiment 2:
(1) 90mg soybean lecithin, 90mg hydrogenated soy phosphatidyl choline, 45mg cholesterol, 10mg vitamin C are accurately weighed,
Mixing, is dissolved in the dehydrated alcohol of 10mL, and water bath sonicator 3min obtains mixture up to being completely dissolved under the conditions of 300W.
(2) mixture for obtaining step (1) depressurizes (vacuum degree 0.08MPa) rotary evaporation under 45 DEG C of water bath conditions
40min removes ethyl alcohol, until forming one layer of uniform liposome membrane in revolving bottle wall.
(3) into revolving bottle be added 20mL 0.05mol/L phosphate buffer (pH=7.4) and 5mgEGCG,
The compound of 10mg vitamin E, 5mg albumin and 5mg resveratrol, rotation hydration 45min, obtains under 45 DEG C of water bath condition
To the thick suspension of liposome.
(4) the low-ester pectin aqueous solution for configuring the 0.3g/100mL of 20mL, is added drop-wise to pectin solution for the thick suspension of liposome
In, it is mixed according to volume ratio 1:1, is finally placed in ice-bath ultrasonic 5min in probe type ultrasonication instrument (1s/1s, 500W) and obtains
The stable composite phospholipid liposome of low-ester pectin.
Comparative example 2:
(1) 180mg soybean lecithin, 45mg cholesterol, 5mg resveratrol, 10mg vitamin C are accurately weighed, is mixed, it is molten
In the dehydrated alcohol of 10mL, water bath sonicator 3min obtains mixture up to being completely dissolved under the conditions of 300W.
(2) mixture for obtaining step (1) depressurizes (vacuum degree 0.08MPa) rotary evaporation under 45 DEG C of water bath conditions
40min removes ethyl alcohol, until forming one layer of uniform liposome membrane in revolving bottle wall.
(3) into revolving bottle be added 20mL 0.05mol/L phosphate buffer (pH=7.4) and 5mgEGCG and
10mg vitamin E, rotation hydration 45min, obtains the thick suspension of liposome under 45 DEG C of water bath condition.
(4) the thick suspension of liposome is placed in ice-bath ultrasonic 5min in probe type ultrasonication instrument (1s/1s, 500W) can be obtained rouge
Plastid.
Embodiment 3:
(1) 100mg soybean lecithin, 100mg hydrogenated soy phosphatidyl choline, 50mg cholesterol, 10mg vitamin are accurately weighed
C, mixing, is dissolved in the dehydrated alcohol of 10mL, and water bath sonicator 3min obtains mixture up to being completely dissolved under the conditions of 300W.
(2) mixture for obtaining step (1) depressurizes (vacuum degree 0.08MPa) rotary evaporation under 45 DEG C of water bath conditions
40min removes ethyl alcohol, until forming one layer of uniform liposome membrane in revolving bottle wall.
(3) into revolving bottle be added 20mL 0.05mol/L phosphate buffer (pH=7.4) and 5mgEGCG,
The compound of 10mg vitamin E, 5mg albumin and 5mg resveratrol, rotation hydration 45min, obtains under 45 DEG C of water bath condition
To the thick suspension of liposome.
(4) the low-ester pectin aqueous solution for configuring the 0.2g/100mL of 20mL, is added drop-wise to pectin solution for the thick suspension of liposome
In, it is mixed according to volume ratio 1:1, is finally placed in ice-bath ultrasonic 5min in probe type ultrasonication instrument (1s/1s, 500W) and obtains
The stable composite phospholipid liposome of low-ester pectin.
Comparative example 3:
(1) 200mg soybean lecithin, 50mg cholesterol, 5mg resveratrol, 10mg vitamin C are accurately weighed, is mixed, it is molten
In the dehydrated alcohol of 10mL, water bath sonicator 3min obtains mixture up to being completely dissolved under the conditions of 300W.
(2) mixture for obtaining step (1) depressurizes (vacuum degree 0.08MPa) rotary evaporation under 45 DEG C of water bath conditions
40min removes ethyl alcohol, until forming one layer of uniform liposome membrane in revolving bottle wall.
(3) into revolving bottle be added 20mL 0.05mol/L phosphate buffer (pH=7.4) and 5mgEGCG and
10mg vitamin E, rotation hydration 45min, obtains the thick suspension of liposome under 45 DEG C of water bath condition.
(4) the thick suspension of liposome is placed in ice-bath ultrasonic 5min in probe type ultrasonication instrument (1s/1s, 500W) can be obtained rouge
Plastid.
Each performance indicator of liposome prepared in the above embodiments measures as follows:
1, with the sustained release and percolation ratio situation of HPLC measurement liposome.
2, inoxidizability: DPPH clearance rate measurement.
Claims (10)
1. a kind of preparation method for the composite phospholipid liposome that low-ester pectin is stable carries out in accordance with the following steps:
(1) soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, vitamin C mixing are weighed, is dissolved in dehydrated alcohol, water-bath is super
Sound obtains mixture up to being completely dissolved；Wherein soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, ascorbic feed intake
Mass ratio is 8-10:8-10:4-5:1, and the volumetric usage of dehydrated alcohol is calculated as 0.8-1.2L/g with ascorbic quality dosage；
(2) mixture that step (1) obtains is depressurized under 40-50 DEG C of water bath condition rotary evaporation and removes ethyl alcohol, until revolving
One layer of uniform liposome membrane is formed in bottle wall；
(3) phosphate buffer and mass ratio that pH=7.4,0.05mol/L are added into revolving bottle are 1:2:1:1's
EGCG, vitamin E, albumin and resveratrol compound, under 40-50 DEG C of water bath condition rotation hydration 30-60min,
The thick suspension of liposome is obtained, wherein the volumetric usage of phosphate buffer is calculated as 4-5L/g with the quality dosage of resveratrol, white
Veratryl alcohol and ascorbic mass ratio are 0.4-0.6:1；
(4) the low-ester pectin aqueous solution for configuring 0.1-0.6g/100mL, the thick suspension of liposome is added drop-wise in pectin solution, according to
Volume 1:1 is mixed, and most obtains the stable composite phospholipid liposome of low-ester pectin through ice-bath ultrasonic afterwards.
2. preparation method as described in claim 1, it is characterised in that: in step (1), the volumetric usage of dehydrated alcohol is to tie up life
The quality dosage of plain C is calculated as 1.0-1.2L/g.
3. preparation method as described in claim 1, it is characterised in that: in step (1), water bath sonicator condition are as follows: in 300w item
Ultrasound 3min under part.
4. preparation method as described in claim 1, it is characterised in that: in step (2), vacuum rotary steam evaporation conditions are as follows: true
Revolving evaporation 40min under the conditions of reciprocal of duty cycle 0.08MPa.
5. preparation method as claimed in claim 4, it is characterised in that: in step (2), bath temperature is 45 DEG C.
6. preparation method as described in claim 1, it is characterised in that: in step (3), the resveratrol with it is ascorbic
Mass ratio is 0.5:1.
7. preparation method as described in claim 1, it is characterised in that: in step (3), the volume of the phosphate buffer is used
Amount is calculated as 4L/g with the quality dosage of resveratrol.
8. preparation method as described in claim 1, it is characterised in that: in step (3), bath temperature is 45 DEG C, rotation hydration
Time is 45min.
9. preparation method as described in claim 1, it is characterised in that: in step (4), the concentration of low-ester pectin aqueous solution is
0.2-0.3g/100mL。
10. preparation method as described in claim 1, it is characterised in that the preparation method carries out in accordance with the following steps:
(1) soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, vitamin C mixing are weighed, is dissolved in dehydrated alcohol, water-bath is super
Sound obtains mixture up to being completely dissolved；Wherein soybean lecithin, hydrogenated soy phosphatidyl choline, cholesterol, ascorbic feed intake
Mass ratio is 8-10:8-10:4-5:1, and the volumetric usage of dehydrated alcohol is calculated as 1.0-1.2L/g with ascorbic quality dosage；
(2) mixture for obtaining step (1) depressurizes rotary evaporation under 45 DEG C of water bath conditions and removes ethyl alcohol, until revolving bottle wall
One layer of uniform liposome membrane of upper formation；
(3) phosphate buffer and mass ratio that pH=7.4,0.05mol/L are added into revolving bottle are 1:2:1:1's
EGCG, vitamin E, albumin and resveratrol compound, under 45 DEG C of water bath condition rotation hydration 45min, obtain rouge
The thick suspension of plastid, wherein the volumetric usage of phosphate buffer is calculated as 4L/g with the quality dosage of resveratrol, resveratrol with
Ascorbic mass ratio is 0.5:1；
(4) the low-ester pectin aqueous solution for configuring 0.2-0.3g/100mL, the thick suspension of liposome is added drop-wise in pectin solution, according to
Volume 1:1 is mixed, and most obtains the stable composite phospholipid liposome of low-ester pectin through ice-bath ultrasonic afterwards.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110200829A (en) * | 2019-05-17 | 2019-09-06 | 湖南人文科技学院 | A kind of resveratrol nanometer alcohol plastid and the preparation method and application thereof |
| CN110250495A (en) * | 2019-06-12 | 2019-09-20 | 浙江工商大学 | A kind of Vc liposome hydrogel and preparation method thereof |
| CN112956680A (en) * | 2021-02-10 | 2021-06-15 | 青岛农业大学 | Plant oil body-imitated core-shell liposome and preparation method thereof |
| CN115024450A (en) * | 2022-06-23 | 2022-09-09 | 四川天晟制药有限公司 | Preparation method of roxburgh rose juice composite embedded beverage |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658499A (en) * | 2009-09-27 | 2010-03-03 | 姚晶萍 | Preparation method of freeze-dried resveratrol albumin powder |
| CN105125494A (en) * | 2015-09-15 | 2015-12-09 | 常州市鼎日环保科技有限公司 | Preparation method of ficus pumila pectin cladded vitamin C nano lipidosome |
| CN107802614A (en) * | 2017-12-11 | 2018-03-16 | 中国药科大学 | A kind of oral albumin nano granular in intestines and stomach with high stability |
| CN108096225A (en) * | 2018-01-03 | 2018-06-01 | 华南理工大学 | A kind of nano fibrous membrane of the liposome containing calcitonin and its preparation method and application |
-
2018
- 2018-08-30 CN CN201811005216.1A patent/CN109498576A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658499A (en) * | 2009-09-27 | 2010-03-03 | 姚晶萍 | Preparation method of freeze-dried resveratrol albumin powder |
| CN105125494A (en) * | 2015-09-15 | 2015-12-09 | 常州市鼎日环保科技有限公司 | Preparation method of ficus pumila pectin cladded vitamin C nano lipidosome |
| CN107802614A (en) * | 2017-12-11 | 2018-03-16 | 中国药科大学 | A kind of oral albumin nano granular in intestines and stomach with high stability |
| CN108096225A (en) * | 2018-01-03 | 2018-06-01 | 华南理工大学 | A kind of nano fibrous membrane of the liposome containing calcitonin and its preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| 中国茶叶学会: "《茶学 学科发展报告》", 30 April 2010, 中国科学技术出版社 * |
| 傅超美 等: "《药用辅料学》", 31 October 2008, 中国中医药出版社 * |
| 周伟: "果胶包覆维生素C纳米脂质体的制备及其性质研究", 《中国优秀硕士学位论文全文库 工程科技Ⅰ辑》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110200829A (en) * | 2019-05-17 | 2019-09-06 | 湖南人文科技学院 | A kind of resveratrol nanometer alcohol plastid and the preparation method and application thereof |
| CN110200829B (en) * | 2019-05-17 | 2022-04-01 | 湖南人文科技学院 | Resveratrol nano ethosome and preparation method and application thereof |
| CN110250495A (en) * | 2019-06-12 | 2019-09-20 | 浙江工商大学 | A kind of Vc liposome hydrogel and preparation method thereof |
| CN110250495B (en) * | 2019-06-12 | 2023-09-08 | 浙江工商大学 | Vc liposome hydrogel and preparation method thereof |
| CN112956680A (en) * | 2021-02-10 | 2021-06-15 | 青岛农业大学 | Plant oil body-imitated core-shell liposome and preparation method thereof |
| CN115024450A (en) * | 2022-06-23 | 2022-09-09 | 四川天晟制药有限公司 | Preparation method of roxburgh rose juice composite embedded beverage |
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