CN109497541A - A method of ferritin-polyphenol embedded object is prepared using low urea - Google Patents
A method of ferritin-polyphenol embedded object is prepared using low urea Download PDFInfo
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- CN109497541A CN109497541A CN201710844770.8A CN201710844770A CN109497541A CN 109497541 A CN109497541 A CN 109497541A CN 201710844770 A CN201710844770 A CN 201710844770A CN 109497541 A CN109497541 A CN 109497541A
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- polyphenol
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- urea
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- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 239000004202 carbamide Substances 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 25
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 33
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 32
- 238000000502 dialysis Methods 0.000 claims abstract description 9
- 230000006698 induction Effects 0.000 claims abstract description 8
- 239000012153 distilled water Substances 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims abstract description 5
- 102000008857 Ferritin Human genes 0.000 claims description 20
- 108050000784 Ferritin Proteins 0.000 claims description 20
- 238000008416 Ferritin Methods 0.000 claims description 20
- 239000000243 solution Substances 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 6
- 239000007993 MOPS buffer Substances 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 239000012149 elution buffer Substances 0.000 claims description 3
- 238000005374 membrane filtration Methods 0.000 claims description 3
- 238000005352 clarification Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 14
- 235000013305 food Nutrition 0.000 abstract description 10
- 239000011149 active material Substances 0.000 abstract description 3
- 239000002537 cosmetic Substances 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000000116 mitigating effect Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- WMBWREPUVVBILR-WIYYLYMNSA-N (-)-Epigallocatechin-3-o-gallate Chemical compound O([C@@H]1CC2=C(O)C=C(C=C2O[C@@H]1C=1C=C(O)C(O)=C(O)C=1)O)C(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-WIYYLYMNSA-N 0.000 description 7
- WMBWREPUVVBILR-UHFFFAOYSA-N GCG Natural products C=1C(O)=C(O)C(O)=CC=1C1OC2=CC(O)=CC(O)=C2CC1OC(=O)C1=CC(O)=C(O)C(O)=C1 WMBWREPUVVBILR-UHFFFAOYSA-N 0.000 description 7
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000000975 bioactive effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 238000001338 self-assembly Methods 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000004153 renaturation Methods 0.000 description 2
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910001139 Telluric iron Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003471 anti-radiation Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229910001447 ferric ion Inorganic materials 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005428 food component Substances 0.000 description 1
- 239000005452 food preservative Substances 0.000 description 1
- 235000019249 food preservative Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002091 nanocage Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000000974 natural food coloring agent Substances 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 1
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 1
- 235000005493 rutin Nutrition 0.000 description 1
- 229960004555 rutoside Drugs 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/045—Organic compounds containing nitrogen as heteroatom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
Abstract
Ferritin-polyphenol embedded object method is prepared using low urea the invention discloses a kind of, comprising the following steps: liquor ferri albuminati is placed in centrifuge tube, adds urea liquid, room temperature is protected from light induction and is incubated for；Natural polyphenol substance is taken, distilled water is dissolved in；Liquor ferri albuminati after polyphenol solution and urea induction is mixed, 4 DEG C are protected from light stirring, and above-mentioned solution is protected from light to dialysis in 4 DEG C of chromatography cabinets and crosses film up to uniform ferritin-polyphenol embedded object solution.Final products are a kind of novel ferritin-natural polyphenol substance embedded object preparation methods, the method reference of one kind more mitigation, low cost, environmental protection is provided for the other food source active materials of embedding, in food, medicine and cosmetic field with very high practical application and promotional value.
Description
Technical field
A method of ferritin-polyphenol embedded object being prepared using low urea, belongs to food technology field.
Background technique
Plant polyphenol is widely present in plant, and application field is extensive, and extraction process is mature, and bioactive functions are many
The multiple functions activity such as more including anti-oxidant, antitumor, anti-aging, anti-radiation and prevention cardiovascular and cerebrovascular disease.Currently, more
Phenol has been applied to industrial many fields, such as: natural food colorant, food preservative, cosmetics etc..However,
Since polyphenolic substance has active phenolic hydroxyl structure, under the conditions ofs being not suitable for pH, high-moisture activity, illumination, high temperature etc. easily
The reactions such as oxidation, polymerization, condensation occur, so as to cause the reduction of stability and the decline of bioactivity.On the other hand, human body is taken the photograph
The a variety of food components entered will also tend to the absorption on polyphenol and generate apparent influence such as protein, carbohydrate, lipid.
Therefore, the stability for how keeping polyphenols and the method for improving its bioavilability are probed into, is current food nutrition
The hot spot and problem of area research, always by the concern of domestic and foreign scholars.
Ferritin is a kind of native iron storage protein being widely present in animal, plant and microorganism.Due to its uniqueness
Spherical hollow shell structure and reversible self assembly characteristic, can be used for the supported of food source bioactive substance molecule.Ferritin is by 24
A subunit composition, every two subunit is antiparallel to form one group, constitutes an approximate regular octahedron, at 4-3-2 solid axle it is symmetrical in
Empty globular molecule, each ferritin molecules form 12 double axis channels, 8 triple axis channels and 6 quadruple axis channels,
Channel size is easy by external environment, such as the influence of urea, problem factor, so as to cause the increase or diminution of size.Outside
Diameter is 12nm, internal diameter 8nm, and with a thickness of 2nm, the hole of 8nm can accommodate 4500 ferric ions.Utilize the denaturation of ferritin
The reversible self-assembly property of renaturation, adds certain function ingredients in albuminous degeneration system, then these components may quilt when renaturation
It is embedded in ferritin internal cavities, so that the ferritin for realizing function ingredients loads.In food and medicine field, iron is utilized
The reversible self assembly characteristic of albumen successfully embeds the natural polyphenols substances such as procyanidine, rutin.It is noted however, that
It is that may will affect organoleptic attribute, the storage, biology work of active material to the pH of middle sexual stage variation from Extreme acid/alkalinity
Property and physicochemical property, or even cause sample pollute and environmental pollution.Therefore, using a kind of mild technology, iron egg is not being destroyed
Small molecule is embedded in the case where white nanocages, facilitates the solution of these problems.It is low dense that we pass through addition during the experiment
The method inducing ferritin channel of degree urea becomes larger, and embeds polyphenol molecule into ferritin lar nanometric cavities by widened channel
It is interior, to prepare the nanoparticles embedded object that size is uniform, stable.
Using ferritin, polyphenols as raw material, by way of adding low urea inducing ferritin channel and becoming larger
Polyphenol substance is embedded, evenly dispersed ferritin-polyphenol embedded object is prepared, the application of this method is not disclosed at home and abroad
Report, not disclosed use.
Summary of the invention
An object of the present invention, which is to provide, a kind of prepares ferritin-polyphenol embedded object method using low urea.
Using ferritin and polyphenol substance as raw material, become larger to embed polyphenol into receiving using low urea inducing ferritin channel
In rice cavity, ferritin-polyphenol embedded object that size is uniform, stable is prepared, to improve the stability of polyphenol substance, finally
Product is a kind of novel ferritin-natural polyphenol substance embedded object preparation method, to promote application of the polyphenol in food
And a kind of inexpensive, more environmentally friendly method reference is provided for the other food source active materials of embedding, stable state.
The present invention provides a kind of novel ferritin-natural polyphenol substance embedded object preparation methods, including following step
It is rapid: liquor ferri albuminati being placed in centrifuge tube, urea liquid is added, room temperature is protected from light induction and is incubated for；Natural polyphenol substance is taken, it is molten
In distilled water；Liquor ferri albuminati after polyphenol solution and urea induction is mixed, 4 DEG C are protected from light stirring, by above-mentioned solution in 4 DEG C of layers
It is protected from light dialysis in analysis cabinet and crosses film up to uniform ferritin-polyphenol embedded object solution.
Further, the concentration of the liquor ferri albuminati is 1.0 μM, and pH value is 6.8 or so.
Further, it is that 20mM urea liquid room temperature is protected from light induction incubation that concentration is added in above-mentioned liquor ferri albuminati.
Further, it weighs polyphenols and is dissolved in the mother liquor for being prepared into that concentration is 1mM in distilled water.
Further, the molar concentration rate when ferritin and Polyphenols solution for adding urea mix is 1: 150.
Further, ferritin and Polyphenols solution mix 4 DEG C and are protected from light stirring 30min.
Further, the bag filter aperture 10kDa used when dialysis.
Further, dialyzate when dialysis: MOPS buffer (pH 6.8,20mM).
Further, it dialyses three times in 4 DEG C of chromatography cabinets, changes an elution buffer every 8h.
Further, it is clarified with 0.45 μm of hydrophilic cellulose membrane filtration by ferritin-polyphenol embedded object solution.
The beneficial effect of this product is: preparing ferritin-polyphenol embedded object by adding urea induction, method is more slow
With environmental protection, and this embedded object is alternatively arranged as functional food additives and nutritional supplement and uses, and further improves natural more
The bioavailability of phenol substance and ferritin, in addition, the preparation method of this low cost has very high practical application and popularization
Value provides a good method reference for the embedding of other bioactive molecules.
Detailed description of the invention
Fig. 1 is ferritin-natural polyphenol substance embedded object Technology Roadmap.
Fig. 2 is ferritin-natural polyphenol substance embedded object (by taking tea polyphenols EGCG as an example) Technology Roadmap.
Fig. 3 is the electron microscope of ferritin-EGCG embedded object.
Specific embodiment
It will be embodied herein in conjunction with specific attached drawing detailed description of the present invention.It should be noted that in following embodiments
The combination of the technical characteristic or technical characteristic of description should not be considered as isolated, they can be combined with each other to reaching
To superior technique effect.In the drawings of the following embodiments, the same symbol that each attached drawing occurs represent identical feature or
Person's component can be applied in different embodiments.
Examples of implementation are by taking tea polyphenols EGCG as an example
The tea polyphenols EGCG standard items for accurately weighing 20mg, which are dissolved in 43.6ml distilled water (pH 6.8), is prepared into concentration
For the mother liquor of 1mM, 4 DEG C are kept in dark place for use.Simultaneously weigh 1.96g urea be dissolved in 8ml distilled water be prepared into concentration be 4M
Mother liquor.Liquor ferri albuminati (1.0 μM, pH 6.8,5.0ml) is placed in the centrifuge tube of 10mL, it is molten that 25 μ L 4M urea are added
Liquid makes its final concentration of 20mM, is incubated at room temperature 2h.Then (4 DEG C are protected from light guarantor to the EGCG mother liquor of addition 1.5mL in the above solution
Deposit) make ferritin: 1: 150,4 DEG C of molar concentration rate of EGCG are protected from light stirring 30min, mix them thoroughly.Later by solution
It is placed in the bag filter that aperture is 10kDa, is protected from light dialysis three in 4 DEG C of chromatography cabinets using MOPS buffer (pH 6.8,20mM)
It is secondary, an elution buffer is changed every 8h.Urea and unbonded EGCG molecule are then removed in dialysis procedure.Finally
By ferritin-polyphenol embedded object solution by 0.45 μm of hydrophilic cellulose membrane filtration clarification, and kept away under the conditions of being placed on 4 DEG C
Light saves.
Ferritin-polyphenol embedded object method is prepared using low urea the present invention provides a kind of, it is low by adding
The mode inducing ferritin channel of concentration urea becomes larger, and so that EGCG molecule is passed through the channel to become larger and enters ferritin lar nanometric cavities
Interior, thus, it is possible to prepare the uniform embedded object solution of system, for the embeddings of other bioactive molecules, to provide a kind of mode slow
It is referred to economic and environment-friendly thinking, has effectively widened the application range of polyphenols and ferritin in the food industry,
Vast market prospect is all had in field in medicine, foods and cosmetics.
Although having been presented for some embodiments of the present invention herein, it will be appreciated by those of skill in the art that
Without departing from the spirit of the invention, the embodiment of the present invention can be changed.Above-described embodiment is exemplary
, it should not be using the embodiments herein as the restriction of interest field of the present invention.
Claims (8)
1. a kind of prepare ferritin-polyphenol embedded object method using low urea, which comprises the following steps:
Liquor ferri albuminati is placed in centrifuge tube, urea liquid is added, room temperature is protected from light induction and is incubated for certain time；Take natural polyphenol class object
Matter is dissolved in distilled water；Liquor ferri albuminati after polyphenol solution and urea induction is mixed, room temperature is protected from light stirring, by above-mentioned solution
It is protected from light dialysis in 4 DEG C of chromatography cabinets and crosses film up to uniform ferritin-polyphenol embedded object solution.
2. a kind of as claimed in claim 2 prepare ferritin-polyphenol embedded object method using low urea, feature exists
In the concentration of the liquor ferri albuminati is 1.0 μM, and pH value is 6.8 or so.
3. a kind of as claimed in claim 3 prepare ferritin-polyphenol embedded object method using low urea, feature exists
In the molar concentration that low urea solution is added is 20mM, and room temperature, which is protected from light, is incubated for 2h.
4. a kind of as claimed in claim 4 prepare ferritin-polyphenol embedded object method using low urea, feature exists
In molar concentration rate when liquor ferri albuminati and polyphenol solution mix is 1: 150.
5. a kind of as claimed in claim 5 prepare ferritin-polyphenol embedded object method using low urea, feature exists
In mixing time is 30min when liquor ferri albuminati and polyphenol solution mix.
6. a kind of as claimed in claim 6 prepare ferritin-polyphenol embedded object method using low urea, feature exists
In being added after the stirring of polyphenol solution the bag filter aperture 10kDa that uses when chromatographing and being protected from light dialysis in cabinets for 4 DEG C, when dialysis dialyses
Liquid: MOPS buffer (pH6.8,20mM) dialyses three times in 4 DEG C of chromatography cabinets, changes an elution buffer every 8h.
7. a kind of as claimed in claim 7 prepare ferritin-polyphenol embedded object method using low urea, feature exists
In with 0.45 μm of hydrophilic cellulose membrane filtration clarification ferritin-polyphenol embedded object solution.
8. a kind of as claimed in claim 8 prepare ferritin-polyphenol embedded object method using low urea, feature exists
In the method preparation that the ferritin that the size is uniform, stable-polyphenol embedding objects system passes through any one of claim 1-7
It obtains.
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| CN201710844770.8A CN109497541A (en) | 2017-09-15 | 2017-09-15 | A method of ferritin-polyphenol embedded object is prepared using low urea |
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| CN201710844770.8A CN109497541A (en) | 2017-09-15 | 2017-09-15 | A method of ferritin-polyphenol embedded object is prepared using low urea |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111150063A (en) * | 2020-01-07 | 2020-05-15 | 天津科技大学 | Method for simultaneously improving hydrophobicity and stability of water-soluble active ingredients by using cage-shaped plant ferritin and application |
| CN112094861A (en) * | 2020-10-26 | 2020-12-18 | 南京林业大学 | Expression and purification method of green alga plant ferritin and application thereof |
-
2017
- 2017-09-15 CN CN201710844770.8A patent/CN109497541A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111150063A (en) * | 2020-01-07 | 2020-05-15 | 天津科技大学 | Method for simultaneously improving hydrophobicity and stability of water-soluble active ingredients by using cage-shaped plant ferritin and application |
| CN112094861A (en) * | 2020-10-26 | 2020-12-18 | 南京林业大学 | Expression and purification method of green alga plant ferritin and application thereof |
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