CN109490305A - A kind of cell smear detection method - Google Patents
A kind of cell smear detection method Download PDFInfo
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- CN109490305A CN109490305A CN201811559881.5A CN201811559881A CN109490305A CN 109490305 A CN109490305 A CN 109490305A CN 201811559881 A CN201811559881 A CN 201811559881A CN 109490305 A CN109490305 A CN 109490305A
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- urine
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- cell
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- 238000001514 detection method Methods 0.000 title claims abstract description 22
- 239000011521 glass Substances 0.000 claims abstract description 17
- 239000013049 sediment Substances 0.000 claims abstract description 16
- 210000002700 urine Anatomy 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003146 anticoagulant agent Substances 0.000 claims abstract description 7
- 229940127219 anticoagulant drug Drugs 0.000 claims abstract description 7
- 238000005119 centrifugation Methods 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 4
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 239000006228 supernatant Substances 0.000 claims abstract description 4
- 238000004043 dyeing Methods 0.000 claims description 12
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 3
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 3
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 3
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 3
- 244000248349 Citrus limon Species 0.000 claims 2
- 235000005979 Citrus limon Nutrition 0.000 claims 2
- 239000000243 solution Substances 0.000 claims 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 230000001476 alcoholic effect Effects 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 230000035484 reaction time Effects 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 235000015424 sodium Nutrition 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 15
- 238000010186 staining Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 235000011083 sodium citrates Nutrition 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011206 morphological examination Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000009603 uroscopy Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of cell smear detection methods, comprising the following steps: S1: material prepares: extracting suitable early morning mud-stream urine, urine is injected in container, suitable anti-coagulants is then injected in a reservoir, obtains mixed liquor；S2: centrifugal treating: mixed liquor is added in centrifuge, carries out centrifugally operated, after the completion of centrifugation, is removed supernatant, is obtained sediment；S3: preparation of specimen: sediment is drawn by suction pipe, then the sediment that suction pipe is drawn is injected on glass slide, then is covered on glass slide with coverslip, pulls coverslip in the opposite direction, sample is made；S4: sample is fixed: suitable ethanol solution is instilled on sample obtained.The present invention can effectively maintain the form of cell in urine, and cell distribution in urine is made more evenly, to be conducive to the classification of cell in urine, greatly improve the accuracy that cytology detects in urine, smear is uniform, bright-coloured after cell color, well-balanced, clear in structure.
Description
Technical field
The present invention relates to cell smear technical field more particularly to a kind of cell smear detection methods.
Background technique
With the continuous development of science, interdisciplinary to interpenetrate, hematology field constantly expands, and detection means is more and more
It is abundant.The inspections such as cellular biochemistry, immune, heredity, cyto-dynamics and Electronic Speculum make morphological examination reach molecular level,
And in clinical everyday work, the cytomorphology inspection under light microscopic is preferred and essential means.Uroscopy can
For certain infectious diseases, current smear cells are unevenly distributed, accumulate excessively, and mucus, blood or inflammatory cell cover different
Normal cell, the normal Multi-layers distributing of cell, abnormal cell often have overlapping phenomenon, influence to observe, therefore we have proposed a kind of paintings of cell
Chip detection method is for solving the above problems.
Summary of the invention
Technical problems based on background technology, the invention proposes a kind of cell smear detection methods.
A kind of cell smear detection method proposed by the present invention, comprising the following steps:
S1: material prepares: extracting suitable early morning mud-stream urine, urine is injected in container, is then injected in a reservoir
Suitable anti-coagulants, obtains mixed liquor；
S2: centrifugal treating: mixed liquor is added in centrifuge, carries out centrifugally operated, after the completion of centrifugation, removes supernatant
Liquid obtains sediment；
S3: preparation of specimen: sediment is drawn by suction pipe, the sediment that suction pipe is drawn then is injected into glass slide
On, then covered on glass slide with coverslip, coverslip is pulled in the opposite direction, and sample is made；
S4: sample is fixed: instilling suitable ethanol solution on sample obtained, processing is fixed to sample；
S5: micro- sem observation: the sample after fixation being put under microscope and carries out observation processing, checks that sample obtained is
No qualification can carry out next operation after observation is qualified；
S6: dyeing processing: sample obtained is put into dyeing liquor and carries out dyeing processing, is compared by coloration result
Detection.
Preferably, in the S1, anti-coagulants is one of sodium citrate, sodium dihydrogen phosphate and disodium ethylene diamine tetraacetate
Or a variety of mixture.
Preferably, in the S1, the time of hybrid reaction is set as 35-55s.
Preferably, in the S2, the revolving speed of centrifuge is set as 200-2800r/min, and centrifugation time is set as 5-8min.
Preferably, in the S3, the angle of the glass slide and coverslip is 30 °, and the sediment in suction pipe is instilled one
It drips on glass slide.
Preferably, in the S4,2-3 drop ethanol solution is instilled, until regular time is set as 35- after instillation
45min。
Preferably, it in the S5, whether to be uniformly distributed on glass slide by microscope main detection sediment, distribution is not
It is uniform then unqualified.
Preferably, in the S6, dyeing uses pap staining.
Beneficial effects of the present invention:
The form that cell in urine can effectively be maintained makes cell distribution in urine more evenly, be conducive to cell in urine
Classification greatly improves the accuracy that cytology in urine detects, and smear is uniform, bright-coloured after cell color, well-balanced, structure is clear
It is clear, using pap staining, have many advantages, such as that nucleus and cytoplasm comparison are clear, transparency is good.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment
A kind of cell smear detection method is proposed in the present embodiment, comprising the following steps: S1: material prepares: extracting suitable
Urine is injected in container, then injects suitable anti-coagulants in a reservoir, obtain mixed liquor by the early morning mud-stream urine of amount；S2:
Centrifugal treating: mixed liquor is added in centrifuge, carries out centrifugally operated, after the completion of centrifugation, is removed supernatant, is precipitated
Object；S3: preparation of specimen: sediment is drawn by suction pipe, then the sediment that suction pipe is drawn is injected on glass slide, then uses
Coverslip covers on glass slide, pulls coverslip in the opposite direction, and sample is made；S4: sample is fixed: on sample obtained
Suitable ethanol solution is instilled, processing is fixed to sample；S5: the sample after fixation micro- sem observation: is put into microscope
Under carry out observation processing, check whether sample obtained qualified, next operation can be carried out after observation is qualified；S6: dyeing processing:
Sample obtained is put into dyeing liquor and carries out dyeing processing, detection is compared by coloration result.
In the present embodiment, in S1, anti-coagulants is one of sodium citrate, sodium dihydrogen phosphate and disodium ethylene diamine tetraacetate
Or a variety of mixtures, in S1, the time of hybrid reaction is set as 35-55s, and in S2, the revolving speed of centrifuge is set as 200-
2800r/min, centrifugation time are set as 5-8min, and in S3, the angle of glass slide and coverslip is 30 °, by the precipitating in suction pipe
Object instills one and drips on glass slide, in S4, instills 2-3 drop ethanol solution, until regular time is arranged after instillation
For 35-45min, in S5, whether to be uniformly distributed, be unevenly distributed then not on glass slide by microscope main detection sediment
Qualification, in S6, dyeing uses pap staining.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of cell smear detection method, which comprises the following steps:
S1: material prepares: extracting suitable early morning mud-stream urine, urine is injected in container, then injection is appropriate in a reservoir
Anti-coagulants, obtain mixed liquor；
S2: centrifugal treating: mixed liquor is added in centrifuge, carries out centrifugally operated, after the completion of centrifugation, is removed supernatant, is obtained
To sediment；
S3: preparation of specimen: sediment being drawn by suction pipe, then the sediment that suction pipe is drawn is injected on glass slide, then
It is covered on glass slide with coverslip, pulls coverslip in the opposite direction, sample is made；
S4: sample is fixed: instilling suitable ethanol solution on sample obtained, processing is fixed to sample；
S5: micro- sem observation: the sample after fixation being put under microscope and carries out observation processing, checks whether sample obtained closes
Lattice can carry out next operation after observation is qualified；
S6: dyeing processing: sample obtained is put into dyeing liquor and carries out dyeing processing, inspection is compared by coloration result
It surveys.
2. a kind of cell smear detection method according to claim 1, which is characterized in that in the S1, anti-coagulants is lemon
One of lemon acid sodium, sodium dihydrogen phosphate and disodium ethylene diamine tetraacetate or a variety of mixtures.
3. a kind of cell smear detection method according to claim 1, which is characterized in that in the S1, hybrid reaction
Time is set as 35-55s.
4. a kind of cell smear detection method according to claim 1, which is characterized in that in the S2, centrifuge turns
Speed is set as 200-2800r/min, and centrifugation time is set as 5-8min.
5. a kind of cell smear detection method according to claim 1, which is characterized in that in the S3, the glass slide
Angle with coverslip is 30 °, and the sediment in suction pipe is instilled one and is dripped on glass slide.
6. a kind of cell smear detection method according to claim 1, which is characterized in that in the S4, instill 2-3 drop second
Alcoholic solution, until regular time is set as 35-45min after instillation.
7. a kind of cell smear detection method according to claim 1, which is characterized in that in the S5, pass through microscope
Uniformly whether main detection sediment be distributed on glass slide, is unevenly distributed then unqualified.
8. a kind of cell smear detection method according to claim 1, which is characterized in that in the S6, dyeing use bar
Albert'stain Albert.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811559881.5A CN109490305A (en) | 2018-12-12 | 2018-12-12 | A kind of cell smear detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811559881.5A CN109490305A (en) | 2018-12-12 | 2018-12-12 | A kind of cell smear detection method |
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| Publication Number | Publication Date |
|---|---|
| CN109490305A true CN109490305A (en) | 2019-03-19 |
Family
ID=65710866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811559881.5A Pending CN109490305A (en) | 2018-12-12 | 2018-12-12 | A kind of cell smear detection method |
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| Country | Link |
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| CN (1) | CN109490305A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1967196A (en) * | 2006-09-14 | 2007-05-23 | 绵竹市人民医院 | Simplified thin-layer liquid-based cytology detection method |
| CN103471892A (en) * | 2013-09-22 | 2013-12-25 | 厦门大学附属第一医院 | Method for preparing pulled type cervical smear |
| CN106053167A (en) * | 2016-05-19 | 2016-10-26 | 四川金域医学检验中心有限公司 | Preparation method of marrow fluid smear |
-
2018
- 2018-12-12 CN CN201811559881.5A patent/CN109490305A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1967196A (en) * | 2006-09-14 | 2007-05-23 | 绵竹市人民医院 | Simplified thin-layer liquid-based cytology detection method |
| CN103471892A (en) * | 2013-09-22 | 2013-12-25 | 厦门大学附属第一医院 | Method for preparing pulled type cervical smear |
| CN106053167A (en) * | 2016-05-19 | 2016-10-26 | 四川金域医学检验中心有限公司 | Preparation method of marrow fluid smear |
Non-Patent Citations (1)
| Title |
|---|
| 张文丽 等: "《医学病理技术与诊断基础（上）》", 30 June 2017, 吉林科学技术出版社 * |
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Application publication date: 20190319 |
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