CN109486697A - A kind of method of bacillus licheniformis and its preparation and processing waste water - Google Patents
A kind of method of bacillus licheniformis and its preparation and processing waste water Download PDFInfo
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- CN109486697A CN109486697A CN201710843888.9A CN201710843888A CN109486697A CN 109486697 A CN109486697 A CN 109486697A CN 201710843888 A CN201710843888 A CN 201710843888A CN 109486697 A CN109486697 A CN 109486697A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/10—Bacillus licheniformis
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
A kind of method that the present invention discloses bacillus licheniformis and its preparation and processing waste water.The bacillus licheniformis is Bacillus licheniformis PPL-SSC6, is preserved in China typical culture collection center, and deposit number is CCTCC NO:M2017200.Bacillus licheniformis PPL-SSC6 of the present invention has the function of biodetergents, and no pollution to the environment can be used for the processing of landfill leachate and sanitary wastewater.Bacterium solution made of the bacillus licheniformis PPL-SSC6, bacterium powder or particle form preparation are added in landfill leachate, can remove landfill leachate stink interior for 24 hours, so that water is formed layering, become to clarify.Preparation made of the bacillus licheniformis PPL-SSC6 is added in sanitary wastewater, can be by dirty material flocculation sedimentation in sanitary wastewater in 10min-24h, and removes odors.
Description
Technical field
The present invention relates to purifying domestic sewage technical field more particularly to a kind of bacillus licheniformis and its preparation and processing
The method of waste water.
Background technique
The processing of percolated liquid in city refuse landfill is always very intractable in Landfill Site Design, operation and management asks
Topic.Percolate is product of the liquid in landfill yard gravity flowing, is mainly derived from the interior aqueous of precipitation and rubbish itself.Due to liquid
Body may influence the property of percolate, including physical factor, chemical factor and biology in many of flow process factor
Factor etc., so the property of percolate changes in a sizable range.In general, pH value is changed between 4~9
Oxygen demand (COD) is learned in the range of 2000~62000mg/L, biochemical oxygen demand (BOD) (BOD5) is 60~45000mg/L, heavy metal
The concentration of heavy metal is almost the same in concentration and municipal wastewater.Percolated liquid in city refuse landfill is a kind of the highly concentrated of complicated component
Organic wastewater is spent, environment is directly discharged into if untreated, will cause serious environmental pollution.The processing method of landfill leachate
Including physical-chemical process and bioanalysis.Physical-chemical process mainly active charcoal absorption, chemical precipitation, Density Separation, chemical oxidation,
A variety of methods such as electronation, ion exchange, film dialysis, air lift and wet oxidation process, when COD is 2000~4000mg/L,
The COD removal rate of physical chemistry method is up to 50%~87%.It is compared with biological treatment, the shadow that materializing strategy is not changed by water quality and quantity
It rings, effluent quality is more stable, and the rubbish that (0.07~0.20) especially lower to BOD5/COD ratio is difficult to biological treatment seeps
Filtrate has preferable treatment effect.But physical chemistry method processing cost is higher, is unsuitable for the processing of big yield landfill leachate, because
This current landfill leachate mainly uses bioanalysis.
Bioanalysis is divided into the combination of Aerobic biological process, anaerobic bio-treated and the two.Aerobic biological process includes living
Property sludge, aeration oxidation pool, the aerobic stabilization pool, biological rotating disk and trickling filter etc..Anaerobic bio-treated includes upward flow sludge
Bed, anaerobic immobilized bioreactor, mixing reactor and the anaerobic stabilization pool.
In recent years, more achievement is achieved in the research of microbiological treatment sanitary wastewater in the world, the technology investment,
Operation, operational administrative and mud recycling, waste water recycling etc. are superior to traditional administering method, before showing wide application
Scape.
Therefore, the present invention provides the biochemical treatment of a kind of bacillus licheniformis processing landfill leachate and sanitary sewage newly side
Method.Bacillus licheniformis (Bacillus licheniformis PPL-SSC6) is the one kind being separated in landfill leachate
New bacillus licheniformis finds no (Bacillus licheniformis PPL-SSC6) as rubbish in the prior art
The report of percolate and sanitary sewage de-sludging bacterium.
Summary of the invention
In view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of bacillus licheniformis and its preparation with
The method for handling waste water, it is intended to which it is higher to solve existing physical chemistry method processing cost, is unsuitable for big yield landfill leachate treatment
Problem.
Technical scheme is as follows:
A kind of bacillus licheniformis, wherein the bacillus licheniformis is Bacillus licheniformis PPL-
SSC6, is preserved in China typical culture collection center, and deposit number is CCTCC NO:M2017200.
The bacillus licheniformis, wherein the bacillus licheniformis is Gram-positive, the bacillus licheniformis
For rod-shaped bacillus licheniformis.
A kind of preparation, wherein the preparation includes any bacillus licheniformis as above.
The preparation, wherein the form of the preparation is one of bacterium solution, bacterium powder, particle.
A method of waste water is handled using as above any preparation, wherein includes:
Step A, the COD value and pH value for measuring waste water determine the ratio that preparation is added according to COD value；
Step B, preparation is added in waste water according to the ratio determined, and stirred；
Step C, standing, filtering, processing are completed.
The method of the preparation processing waste water, wherein in the step B, example will system for 1: 10-10000 in mass ratio
Agent is added in waste water.
The method of the described preparation processing waste water, wherein in the step B, the preparation is the 100- at 28-37 DEG C
200rpm shakes the bacterium solution of 24-48h under aerobic conditions.
The method of the preparation processing waste water, wherein in the step B, the time of stirring is 5-15min.
The method of the preparation processing waste water, wherein in the step C, the time of standing is 10min-24h.
The method of the preparation processing waste water, wherein in the step A, using COD content in COD instrument measurement waste water.
The utility model has the advantages that bacillus licheniformis (Bacillus licheniformis PPL-SSC6) of the present invention, has life
Object detergent function, no pollution to the environment can be used for the processing of landfill leachate and sanitary wastewater.Be added this kind of bacterium bacterium solution,
Any form preparation such as bacterium powder or particle is interior for 24 hours to remove waste water stink in waste water, so that water is formed layering, becomes to clarify.
This bacterium is the natural bacteria screened in landfill leachate, non-hazardous to environment.Moreover it is possible to reach cycling use of water and live back
The purpose of receipts.
Specific embodiment
A kind of method that the present invention provides bacillus licheniformis and its preparation and processing waste water, for make the purpose of the present invention,
Technical solution and effect are clearer, clear, and the present invention is described in more detail below.It should be appreciated that described herein
Specific embodiment is only used to explain the present invention, is not intended to limit the present invention.
The present invention provides a kind of bacillus licheniformis, wherein the bacillus licheniformis is Bacillus
Licheniformis PPL-SSC6, being preserved in China typical culture collection center, (address is wuchang, wuhan Luo Ka
Mountain), deposit number is CCTCC NO:M2017200, and the deposit date is on April 21st, 2017.Lichens gemma bar of the present invention
Bacterium can be used as de-sludging bacterium processing landfill leachate and sanitary wastewater, and the bacterium of this kind of bacterium is added by 1: 10~10000 mass ratio
Any form preparation such as liquid, bacterium powder or particle can remove waste water stink in waste water in 10min-24h, water is made to form layering,
Become to clarify.This bacterium is the natural bacteria screened in landfill leachate, non-hazardous to environment.It is used moreover it is possible to reach circulation
The purpose of water and life recycling.
Specifically, bacillus licheniformis of the present invention is Gram-positive, and the bacillus licheniformis is rod-shaped lichens
Bacillus.
The present invention also provides a kind of preparations, wherein the preparation includes any bacillus licheniformis as above.Institute
The form for stating preparation can be one of bacterium solution, bacterium powder, particle, and in other words, the bacillus licheniformis can be made into bacterium solution
Form preparation may be made as bacterium powder form preparation, can be with made into particulate form preparation.
The present invention also provides a kind of methods using as above any preparation processing waste water, wherein includes:
Step A, the COD value and pH value for measuring waste water determine the ratio that preparation is added according to COD value.
The step A determines the ratio that preparation is added according to COD value specifically, using COD content in COD instrument measurement waste water
Example, preferably preparation is added in waste water for 1: 10-10000 for example in mass ratio.The pH value of waste water is measured, adjusting pH value of waste water is
Between 2-5, this is because the optimal pH of bacillus licheniformis work is between 2-5.In other words, when pH value of waste water is in 2-5
When except range, pH value of waste water is first adjusted between 2-5, to ensure that bacillus licheniformis works under optimal pH range.
Step B, preparation is added in waste water according to the ratio determined, and stirred.
The step B is specifically, example is 1: 10-10000 the addition of bacterium solution, bacterium powder or particle form preparation is useless in mass ratio
In water, it is then gently mixed 5-15min (such as 10min).Preferably, the preparation be at 28-37 DEG C, 100-200rpm, it is aerobic
Under the conditions of shake the bacterium solution of 24-48h.The bacteria liquid has egg color, sticky feature.
Step C, standing, filtering, processing are completed.
Then the step C is filtered, supernatant water is taken away, bed mud takes filters pressing away specifically, staticly settle.Wherein, it stands
Time be 10min-24h.
Bacillus licheniformis (Bacillus licheniformis PPL-SSC6) of the present invention has biodetergents function
Can, no pollution to the environment can be used for the processing of landfill leachate and sanitary wastewater.Bacillus licheniformis (the Bacillus
Licheniformis PPL-SSC6) made of bacterium solution, bacterium powder or particle form preparation be added in waste water, in 10min-24h
Can be by dirty material flocculation sedimentation in waste water, and remove odors, life harmful substance contents in water are reduced rapidly, country is reached
Discharge standard.The method of the present invention uses biotechnology, has the characteristics that efficient, quick, time and labour saving, is that one kind can be extensive
The novel sanitary wastewater promoted handles new technology.
Below by embodiment, the present invention is described in detail.
Embodiment 1
Percolate of the sewage from Shenzhen's refuse landfill that the present embodiment uses, is detected index content in its water body
Such as the following table 1:
The waste water index of table 1, percolate
It is handled using the method for invention formulation processing waste water, comprising the following steps:
1,0.1L garbage leachate wastewater is taken；
The bacterium solution 30ml for having shaken the bacillus licheniformis (Bacillus licheniformis PPL-SSC6) of 48h is added,
Stir 10min, quiescent setting for 24 hours after, filtering.
It is 4200 that survey filtrate pH, which is 6.5COD, removal rate 90.7%.
2,0.1L sanitary sewage is taken
The bacterium solution for having shaken the bacillus licheniformis (Bacillus licheniformis PPL-SSC6) of 48h is added
0.1ml, stir 10min, quiescent setting for 24 hours after, filtering.
Surveying filtrate pH is 6.5, COD value 12, removal rate 73.3%.
As a result illustrate: it can be seen from the results above that bacillus licheniformis of the present invention (Bacillus licheniformis
PPL-SSC6) have the function of biodetergents, no pollution to the environment can be used for the processing of landfill leachate and sanitary wastewater.Its
90.7% is reached to the removal rate of COD of waste leachate, 73.3% is reached to the removal rate of common life COD of sewage, and have
Remove the effect of water body peculiar smell.
Strain is described in detail:
Bacillus licheniformis PPL-SSC6
Bacillus licheniformis PPL-SSC6 is present in one of soil and plumage microorganism, is a gram sun
Property, mesophilic bacteria.Its optimum growth temp is about 50 DEG C, but it can survive at higher temperatures.Its secretase is most
Thermophilic degree is 37 DEG C.It can be present in rugged environment with spore form.It has the function of biodetergents.
The distinguished sequence of bacillus licheniformis PPL-SSC6 is as follows:
CTTGCATGCCTGCAGGTCGACGATTAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAA
TACATGCAAGTCGAGCGGACCGACGGGAGCTTGCTCCCTTAGGTCAGCGGCGGACGGGTGAGTAACACGTGGGTAAC
CTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGATTGAGCCGCATGGTTCAATTAT
AAAAGGTGGCTTTCAGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAG
GCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGC
AGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGT
AAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACG
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGC
AGGCGGTTTCTTAAGTCTGGTGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGC
AGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACT
CTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGT
AAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGA
GTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAG
CAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCCCCTTCGGGGGCAGAGTG
ACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATC
TTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAA
TCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGAGGCTAA
GCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATC
GCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACAC
CCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGT
AGCCGTAATCTCTAGAGGATCCCGGGTACCGAGCTCGAAT
In conclusion the method for a kind of bacillus licheniformis provided by the invention and its preparation and processing waste water, the present invention
Bacillus licheniformis PPL-SSC6 has the function of biodetergents, and no pollution to the environment, can be used for landfill leachate and life is useless
The processing of water.Landfill leachate is added in bacterium solution made of the bacillus licheniformis PPL-SSC6, bacterium powder or particle form preparation
In, landfill leachate stink can be removed interior for 24 hours, so that water is formed layering, becomes to clarify.The bacillus licheniformis PPL-
Bacterium solution made of SSC6, bacterium powder or particle form preparation are added in sanitary wastewater, can will be in sanitary wastewater in 10min-24h
Dirty material flocculation sedimentation, and remove odors, life harmful substance contents in water are reduced rapidly, national emission standard is reached.This
Inventive method uses biotechnology, has the characteristics that efficient, quick, time and labour saving, is that one kind can be with the novel of large-scale promotion
Sanitary wastewater handles new technology.
It should be understood that the application of the present invention is not limited to the above for those of ordinary skills can
With improvement or transformation based on the above description, all these modifications and variations all should belong to the guarantor of appended claims of the present invention
Protect range.
Claims (10)
1. a kind of bacillus licheniformis, which is characterized in that the bacillus licheniformis is Bacillus licheniformis
PPL-SSC6, is preserved in China typical culture collection center, and deposit number is CCTCC NO:M2017200.
2. bacillus licheniformis according to claim 1, which is characterized in that the bacillus licheniformis is gram sun
Property, the bacillus licheniformis is rod-shaped bacillus licheniformis.
3. a kind of preparation, which is characterized in that the preparation includes any bacillus licheniformis of claim 1-2.
4. preparation according to claim 3, which is characterized in that the form of the preparation is bacterium solution, bacterium powder, one in particle
Kind.
5. a kind of method using the preparation processing waste water as described in claim 3-4 is any characterized by comprising
Step A, the COD value and pH value for measuring waste water determine the ratio that preparation is added according to COD value；
Step B, preparation is added in waste water according to the ratio determined, and stirred；
Step C, standing, filtering, processing are completed.
6. the method for preparation processing waste water according to claim 5, which is characterized in that in the step B, in mass ratio
Preparation is added in waste water for 1: 10-10000 for example.
7. the method for preparation processing waste water according to claim 5, which is characterized in that in the step B, the preparation
For the bacterium solution for shaking 24-48h under 28-37 DEG C, 100-200rpm, aerobic conditions.
8. the method for preparation according to claim 5 processing waste water, which is characterized in that in the step B, stirring when
Between be 5-15min.
9. the method for preparation according to claim 5 processing waste water, which is characterized in that in the step C, standing when
Between be 10min-24h.
10. the method for preparation processing waste water according to claim 5, which is characterized in that in the step A, using COD
Instrument measures COD content in waste water.
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| CN201710843888.9A CN109486697B (en) | 2017-09-13 | 2017-09-13 | Bacillus licheniformis, preparation thereof and method for treating wastewater |
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| CN109486697B CN109486697B (en) | 2021-05-18 |
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Cited By (1)
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| CN114317382A (en) * | 2022-03-08 | 2022-04-12 | 佛山市玉凰生态环境科技有限公司 | Anaerobic strain applied to riverway water body COD degradation and application thereof |
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| CN103937712A (en) * | 2014-04-04 | 2014-07-23 | 华南理工大学 | Bacillus licheniformis and application thereof |
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| CN105441359A (en) * | 2015-12-17 | 2016-03-30 | 华南理工大学 | Strain of bacillus licheniformis and applications thereof |
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| CN114317382A (en) * | 2022-03-08 | 2022-04-12 | 佛山市玉凰生态环境科技有限公司 | Anaerobic strain applied to riverway water body COD degradation and application thereof |
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