CN109486651A - Separator and its application, detection system, electrochemical detection system and cell sorting system - Google Patents
Separator and its application, detection system, electrochemical detection system and cell sorting system Download PDFInfo
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- CN109486651A CN109486651A CN201811169486.6A CN201811169486A CN109486651A CN 109486651 A CN109486651 A CN 109486651A CN 201811169486 A CN201811169486 A CN 201811169486A CN 109486651 A CN109486651 A CN 109486651A
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- 238000001514 detection method Methods 0.000 title claims abstract description 65
- 238000000835 electrochemical detection Methods 0.000 title claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 52
- 239000012528 membrane Substances 0.000 claims abstract description 45
- 238000001179 sorption measurement Methods 0.000 claims description 92
- 230000005291 magnetic effect Effects 0.000 claims description 63
- 239000007788 liquid Substances 0.000 claims description 55
- 239000002699 waste material Substances 0.000 claims description 47
- 238000004140 cleaning Methods 0.000 claims description 26
- 239000011324 bead Substances 0.000 claims description 21
- 238000002038 chemiluminescence detection Methods 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 102000039446 nucleic acids Human genes 0.000 claims description 8
- 108020004707 nucleic acids Proteins 0.000 claims description 8
- 150000007523 nucleic acids Chemical class 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 4
- 230000005389 magnetism Effects 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 3
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 2
- 238000011109 contamination Methods 0.000 abstract description 7
- 239000013076 target substance Substances 0.000 description 60
- 210000004027 cell Anatomy 0.000 description 32
- 239000003153 chemical reaction reagent Substances 0.000 description 18
- 239000006166 lysate Substances 0.000 description 11
- 239000007795 chemical reaction product Substances 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 230000005611 electricity Effects 0.000 description 3
- 239000003480 eluent Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- 150000001336 alkenes Chemical class 0.000 description 2
- 239000000696 magnetic material Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 229920000098 polyolefin Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000010808 liquid waste Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000002826 magnetic-activated cell sorting Methods 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 239000011325 microbead Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/06—Magnetic means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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Abstract
The present invention relates to a kind of separator and its application, detection system, electrochemical detection system and cell sorting systems.A kind of separator includes cylinder, diaphragm assembly and sliding part, and cylinder has opposite first end and second end；Diaphragm assembly includes multiple separation membranes and multiple plugging films, multiple separation membranes are contained in cylinder and are tightly connected with cylinder, multiple separation membranes are alternatively arranged from second end to first end and separate the cylinder into end chambers and N number of function chamber, end chambers are close to second end, drainage hole is offered on the side wall of N number of function chamber, each drainage hole, which blocks, plugging film；Sliding part is arranged close to first end and can slide to the direction close to second end.Above-mentioned separator is of reduced contamination to sample to be tested.
Description
Technical field
The present invention relates to separator technical fields, more particularly to a kind of separator and its application, detection system, electricity
Chemical detection system and cell sorting system.
Background technique
Paramagnetic particle method isolation technics is mainly to pass through the specific adsorption of magnetic bead and material to be separated to isolate material to be separated,
It is widely used in nucleic acid extraction and chemiluminescence detection because its specificity is stronger, safe and non-toxic.Currently, most of magnetic
In the use process of pearl separator, it is required to sample to be tested being fitted into centrifuge tube to be placed in magnetic bead separating device again and separates
Operation.Such mode need to uncap repeatedly liquid feeding, lid lid mixes and abandons the process of liquid with uncapping, and uncapping repeatedly easily causes to test sample
Product are contaminated.
Summary of the invention
Based on this, it is necessary to provide a kind of pair of sample to be tested separator of reduced contamination.
Further it is provided that a kind of application of separator, detection system, electrochemical detection system and cell sorting system.
A kind of separator, comprising:
Cylinder has opposite first end and second end；
Diaphragm assembly, including multiple separation membranes and multiple plugging films, multiple separation membranes be contained in the cylinder and
Be tightly connected with the cylinder, multiple separation membranes from the second end to the first end be alternatively arranged and by the cylinder
Body is separated into end chambers and N number of function chamber, and the end chambers are close to the second end, the side of N number of function chamber
Drainage hole is offered on wall, each drainage hole, which blocks, the plugging film；And
Sliding part is arranged close to the first end, and can slide to the direction close to the second end.
In above-mentioned separator, end chambers and N number of function chamber are separated the cylinder by multiple separation membranes, and multiple
Separation membrane is tightly connected with cylinder, offers drainage hole on the side wall of N number of function chamber, each drainage hole, which blocks, envelope
Stifled film, can place the adsorption piece for adsorbed target substance in the function chamber near first end, can be in other function
Placing response liquid or cleaning solution etc. in energy chamber；Since sliding part is arranged close to first end, and can be to the side close to second end
To sliding, when needing to separate target substance, sliding part can be contained in the function chamber of first end, Ke Yi
Adsorption piece is arranged far from the side of first end in sliding part, and sample to be separated is added and is adsorbed in target substance on adsorption piece,
It slides sliding part to the direction close to second end, so that plugging film and separation membrane rupture, and carries and be adsorbed with target substance
Adsorption piece enters other function chamber and is reacted or cleaned, and eventually enters into end chambers and obtains pure target substance；When need
When detecting target substance, pure target substance can be detected from flowing into detection device in end chambers, it can also be with
Directly detected in end chambers, can also in end chambers placing response reagent or detection reagent so that object
Matter is reacted with reaction reagent or detection reagent, to detect target substance.Above-mentioned separator is simple, without uncapping repeatedly
Realize the separation and detection of target substance, it is easy to operate, it is of reduced contamination to sample to be tested.
The separator further includes impeller in one of the embodiments, and the impeller and the sliding part can
Releasably connect.
It in one of the embodiments, further include adsorption piece, the adsorption piece is contained in the institute near the first end
It states in function chamber, the impeller at least partly has magnetism, impeller promotion when connecting with the sliding part
Part can adsorb the adsorption piece and the adsorption piece is enable to be adsorbed on the sliding part.
The separator further includes for providing the magnetic field hair in magnetic field to the adsorption piece in one of the embodiments,
Life structure, the adsorption piece can be movable under the action of the magnetic field.
The adsorption piece is magnetic bead in one of the embodiments,.
The magnetic bead is superparamagnetism silica nanometer magnetic bead or carboxylated magnetic bead in one of the embodiments,.
In one of the embodiments, further include adsorption piece, the adsorption piece be chip, the adsorption piece be contained near
In the function chamber of the nearly first end, and it is fixed on the sliding part.
The tensile strength of the separation membrane is greater than the tensile strength of the plugging film, and institute in one of the embodiments,
State the indoor pressure of the functional chamber that can make where the sliding part when sliding part is slided to the direction close to the second end
It is strong to increase, and the plugging film of the function chamber where the sliding part and the separation membrane is enable successively to rupture.
It in one of the embodiments, further include waste liquid cabin, the waste liquid cabin is connected to the drainage hole.
The waste liquid cabin is annular hollow structure in one of the embodiments, and the cylinder is at least partially housed in institute
It states in waste liquid cabin, and is tightly connected with the waste liquid cabin；
Or, the waste liquid cabin is tubular, the outer wall of the outer wall and the cylinder in the waste liquid cabin is affixed.
The side wall of the adsorbent chamber is also provided with the air pressure balance communicated with the waste liquid cabin in one of the embodiments,
Hole, the air equalizer opening are arranged close to the first end.
The cylinder offers adding mouth in one of the embodiments, and the adding mouth is located most closely to described first
In the cavity wall of the function chamber at end, the separator further includes the sealing element for sealing the adding mouth.
The application of separator described above in nucleic acid extraction, chemiluminescence detection or cell sorting.
A kind of detection system, including separator described above.
The detection system is PCR detection system in one of the embodiments, and the end chambers are test chamber, most
The function chamber close to the first end is sample cavity, and at least one of remaining described function chamber is cleaning chamber；
Or, the detection system is chemiluminescence detection system, the end chambers are test chamber, near described first
The function chamber at end is sample cavity, and at least one of remaining described function chamber is reaction chamber.
A kind of electrochemical detection system, which is characterized in that including separator described above.
The end chambers are test chamber in one of the embodiments, near the functional chamber of the first end
Room is sample cavity, and at least one of remaining described function chamber is cleaning chamber.
A kind of cell sorting system, which is characterized in that including separator described above.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the separator of an embodiment；
Fig. 2 is that separator shown in FIG. 1 omits the structural schematic diagram behind waste liquid cabin；
Fig. 3 is the structural schematic diagram of sliding part and impeller in separator shown in FIG. 1；
Fig. 4 is that the separator in chemiluminescence detection system omits the structural schematic diagram behind waste liquid cabin.
Specific embodiment
To facilitate the understanding of the present invention, a more comprehensive description of the invention is given in the following sections with reference to the relevant attached drawings.In attached drawing
Give preferred embodiment of the invention.But the invention can be realized in many different forms, however it is not limited to herein
Described embodiment.On the contrary, purpose of providing these embodiments is keeps the understanding to the disclosure more saturating
It is thorough comprehensive.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.
As shown in Figure 1, the detection system of an embodiment includes separator 10 and detection device (not shown).Separation dress
10 are set for isolating the target substance in sample to be tested.Detection device is connected to separator 10, in sample to be tested
Target substance qualitatively or quantitatively detected.
Sample to be tested is blood or saliva in one of the embodiments,.Target substance is nucleic acid and target cell.It needs
Illustrate, sample to be tested be not limited to it is above-mentioned point out sample, target substance be also not necessarily limited to it is above-mentioned point out substance, can be according to reality
Situation is configured.
Separator 10 includes cylinder 100, diaphragm assembly 200, sliding part 300, impeller 400 and waste liquid cabin 600.Scheming
Show in embodiment, target substance is nucleic acid.Separator 10 is used for nucleic acid extraction.
Referring to Figure 2 together, cylinder 100 is the shell of separator 10.Cylinder 100 includes cylinder ontology 110, the first side
Plate 120 and the second side plate 130.Cylinder ontology 110 has opposite first end 111 and second end 113.First end 111 and second
End 113 opens up the first opening (not shown) and the second opening (not shown).First opening is oppositely arranged with the second opening.First side
Plate 120 and the second side plate 130 cover the first opening and the second opening respectively, and are tightly connected with cylinder ontology 110.
It should be noted that cylinder ontology 110 can be an integral molding structure with the second side plate 130, the first side plate 120,
It may be other fixed connection modes, such as weld.
In the illustrated embodiment, cylinder ontology 110 is cylindrical shape.First side plate 120 and the second side plate 130 are disk
Shape.First side plate 120 and the second side plate 130 are opposite and parallel.The internal diameter substantially phase of the outer diameter of first side plate 120 and the first opening
When so that the first side plate 120 just covers the first opening；And second side plate 130 outer diameter and second opening internal diameter substantially phase
When so that the second side plate 130 just covers the second opening.
It should be noted that cylinder ontology 110 is not limited to above-mentioned point out shape, or other shapes, such as square tube
Shape.Correspondingly, the first side plate 120 and the second side plate 130 are also not necessarily limited to above-mentioned point out shape, or other shapes, such as
It is rectangular.It can be configured according to the actual situation, as long as guaranteeing that the first side plate 120 covers first with the second side plate 130 respectively and opens
Mouth and the second opening, and affixed with cylinder ontology 110 and sealed connection.
Diaphragm assembly 200 includes multiple separation membranes 210 and multiple plugging films 220.Multiple separation membranes 210 are contained in cylinder
100, and be tightly connected with cylinder 100.Separation membrane 210 is alternatively arranged from second end 113 to the direction of first end 111 and by cylinder
Body 100 is separated into end chambers 140 and N number of function chamber 150.End chambers 140 are arranged close to second end 113.N number of functional chamber
Drainage hole 160 is offered on the side wall of room 150, each drainage hole 160, which blocks, plugging film 220.
Detection system is PCR detection system in one of the embodiments,.End chambers 140 are test chamber, near the
The function chamber 150 of one end is sample cavity, and at least one of remaining function chamber 150 is cleaning chamber.
In the illustrated embodiment, separation membrane 210 is three.Each separation membrane 210 is disc, and with the first side plate
120 are arranged in parallel.Three separation membranes 210 are alternatively arranged from second end 113 to the direction of first end 111 and separate cylinder 100
At end chambers 140 and three function chambers 150.End chambers 140 are for accommodating eluent.Near the function of first end 111
Energy chamber 150 can accommodate sample to be tested.Other two function chamber 150 is for accommodating cleaning solution to clean target substance.Three
Drainage hole 160 is offered on the side wall of a function chamber 150.Plugging film 220 is three.Three plugging films 220 block respectively
On the drainage hole 160 of three function chambers 150.
It should be noted that it can be identical clear for being used to accommodate the cleaning solution in two function chambers 150 of cleaning solution
Washing lotion, or different cleaning solutions can be configured according to the actual situation.
Separation membrane 210 is acrylic film, nylon membrane, teflon membrane, polyethylene film, poly- third in one of the embodiments,
Alkene film or polyolefin film.It should be noted that separation membrane 210 is not limited to the above-mentioned film pointed out, film commonly used in the art can be with
As separation membrane 210.
Plugging film 220 is acrylic film, nylon membrane, teflon membrane, polyethylene film, poly- third in one of the embodiments,
Alkene film or polyolefin film.It should be noted that plugging film 220 is not limited to the above-mentioned film pointed out, film commonly used in the art can be with
As plugging film 220.
Further, cylinder 100 offers adding mouth 170.Adding mouth 170 is located most closely to the functional chamber of first end 111
In the cavity wall of room 150.Sample to be tested can be added in cylinder 100 from adding mouth 170.Further, separator 10 also wraps
Include the sealing element 500 for sealing adding mouth 170.In the shown embodiment, adding mouth 170 and the interval of drainage hole 160 and phase
To setting.Sealing element 500 is plug, seals adding mouth 170 can fill in adding mouth 170.It should be noted that sealer
500 are not limited to plug, or lid seals adding mouth 170 to be covered on adding mouth 170.
Cylinder 100 offers outlet 190.Outlet 190 is communicated with end chambers 140.Further, separator 10
It further include the disabler (not shown) for sealing outlet 190.In the shown embodiment, outlet 190 is opened in second
Side plate 130, and run through the second side plate 130.Disabler can be plug, can fill in outlet 190 with sealing outlet
190.It should be noted that disabler may be lid, can be covered on outlet 190 with sealing outlet 190.
Sliding part 300 is arranged close to first end 111, and can slide to the direction close to second end 113.Further,
The tensile strength of separation membrane 210 is greater than the tensile strength of plugging film 220.Sliding part 300 is slided to the direction close to second end 113
When the pressure in function chamber 150 where sliding part 300 can be made to increase, and make the function chamber 150 where sliding part 300
Plugging film 220 and separation membrane 210 can successively rupture.Further, sliding part 300 is at least partially housed near
In the function chamber 150 of one end 111 and sealing near first end 111 function chamber 150.
Also referring to Fig. 3, sliding part 300 includes sliding part 310 and the mounting portion 320 affixed with sliding part 310.
Sliding part 310 is arranged close to first end 111, and can slide to the direction close to second end 113.Further,
Sliding part 310 is contained in the function chamber 150 near first end 111 and is tightly connected with the inner wall of cylinder ontology 110.Scheming
Show in embodiment, sliding part 310 is discoid.Sliding part 310 is arranged in parallel with separation membrane 210.
Mounting portion 320 is arranged close to first end 111, and can slide to the direction close to second end 113.Further,
Mounting portion 320 is partially housed in the function chamber 150 of first end 111 and sealed cylinder 100.In illustrated embodiment
In, mounting portion 320 is generally cylindrical.Mounting portion 320 is located at sliding part 310 close to the side of the first side plate 120.Mounting portion
320 one end and sliding part 310 are affixed.
Further, cylinder 100 offers the mounting hole communicated with the function chamber 150 near first end 111 and (schemes not
Show).Sliding part 300 can wear mounting hole and be at least partially housed in the function chamber 150 of first end 111.?
In illustrated embodiment, mounting hole is opened on the first side plate 120.Mounting portion 320 wear mounting hole and be partially housed near
In the function chamber 150 of first end 111.
Impeller 400 and sliding part 300 are detachably connected.Further, impeller 400 can push sliding part 300
Sliding.In the illustrated embodiment, impeller 400 is rod-shaped.Further, mounting portion 320 offers container 322.Container
322 are opened in the one end of mounting portion 320 far from sliding part 310.Impeller 400 can be at least partially housed in container 322,
To push the sliding of sliding part 300.In the illustrated embodiment, the one end of container 322 from mounting portion 320 far from sliding part 310 to
Sunken inside is formed.
Further, separator 10 further includes adsorption piece (not shown).Adsorption piece is contained near first end 111
In function chamber 150.Impeller 400 at least partly has magnetism.400 energy of impeller when impeller 400 is connect with sliding part 300
It is enough to provide magnetic field to adsorption piece, and it is fixed on adsorption piece on sliding part 300.
In the shown embodiment, adsorption piece is contained in the function chamber 150 of first end 111, and is located at and is slided
On dynamic side of the portion 310 far from mounting portion 320.Impeller 400 has magnetism close to the side of sliding part 300, to give adsorption piece
Magnetic field is provided and is capable of fixing adsorption piece on side of the sliding part 310 far from mounting portion 320.It should be noted that pushing
Part 400 can also directly be made of magnetic material.
Adsorption piece is magnetic bead in one of the embodiments,.Further, magnetic bead is superparamagnetism silica nanometer magnetic bead
Or carboxylated magnetic bead.It should be noted that adsorption piece is not limited to the above-mentioned magnetic bead pointed out, or other magnetic beads, Ke Yigen
It is configured according to actual conditions, as long as can be in conjunction with the target substance in sample to be tested.
Separator 10 further includes lysate in one of the embodiments, discharges object to crack sample to be tested
Matter.Lysate is contained in the function chamber 150 of first end 111.Further, it when target substance is nucleic acid, splits
Solving liquid is cell pyrolysis liquid or protein lysate.It, can also be with it should be noted that lysate is not limited to the above-mentioned lysate pointed out
It for other lysates, can be configured according to the actual situation, as long as the target substance in sample to be tested can be made to discharge.
Separator 10 further includes for providing the magnetic field generating means (not shown) in magnetic field to adsorption piece.Adsorption piece can
It is movable under the influence of a magnetic field.By the way that external magnetic field is added, adsorption piece can be made to move to achieve the effect that mixing.Wherein, magnetic
Field generating mechanism can be the magnetic field generating means with electromagnetic coil, or the magnetic field generating means with permanent magnet.
Waste liquid cabin 600 is connected to drainage hole 160.Further, waste liquid cabin 600 is cylindrical shape.The outer wall in waste liquid cabin 600 with
The outer wall of cylinder 100 is affixed.In the shown embodiment, the extending direction of the extending direction Yu cylinder 100 in waste liquid cabin 600 is substantially
In parallel.The outer peripheral surface in waste liquid cabin 600 offers installing port (not shown).The outer wall in waste liquid cabin 600 and the outer wall of cylinder 100 are affixed
And masking installing port, so that waste liquid cabin 600 and cylinder 100 cooperatively form accommodating chamber (not shown).
Further, the side wall of cylinder 100 is also provided with the air equalizer opening 144 communicated with waste liquid cabin 600.Air pressure balance
Hole 144 is arranged close to first end 111.Air equalizer opening 144 is used to balance the air pressure in cylinder 100.When needing near the
When sample to be tested being added in the function chamber 150 of one end 111, sliding part 310 can be made to be located at adding mouth 170 and air equalizer opening
Between 144.
Detection device is connected to separator 10, qualitatively or quantitatively to be detected to target substance in sample to be tested.?
In illustrated embodiment, detection device is PCR detection device.Optionally, detection device is micro-fluidic PCR detection device.Detection dress
Setting can be tightly connected with outlet 190, so that the target substance in end chambers 140 is able to enter in detection device and is examined
It surveys.
The operating process of above-mentioned separator 10 is as follows:
(1) during preparative separation device 10, sliding part 310 is contained in the functional chamber near first end 111
In room 150, and make sliding part 310 between adding mouth 170 and air equalizer opening 144, in the function near first end 111
Adsorption piece is added in chamber 150, and adsorption piece is made to be located at side of the sliding part 310 far from mounting portion 320, in other function chamber
Cleaning solution is added in 150, eluent is added in end chambers 140.It should be noted that if need by sample to be tested crack and
When making target substance release that can just be adsorbed in adsorption piece, lysate is added in the function chamber 150 near first end 111.
(2) it is added in the function chamber 150 of first end 111 from adding mouth 170 by sample to be tested, opens magnetic field hair
Life structure is to provide magnetic field to cylinder 100, so that sample to be tested and adsorption piece mix.If addition has lysate, sample to be tested
It is decomposed under the action of lysate and discharges target substance, target substance and the adsorption piece absorption of release obtain carrying target
The adsorption piece of substance.
(3) after mixing, magnetic field generating means are closed.It is contained in impeller 400 in container 322, to push cunning
Moving part 300 and fixed adsorption piece.Sliding part 300 moves under the drive of impeller 400 to the direction close to second end 113, sliding
The plugging film 220 and separation membrane 210 of function chamber 150 where dynamic portion 310 successively rupture under the influence of air pressure, and waste liquid is from row
Fluid apertures 160 flows into waste liquid cabin 600, and sliding part 300 continues to move, and the adsorption piece for carrying target substance is made to enter next function
In chamber 150, stop motion moves impeller 400 to the direction close to first end 111 and adsorption piece cannot be made to fix.It opens
Magnetic field generating means are opened to provide magnetic field to cylinder 100, so that adsorption piece and cleaning solution mix and clean and carry target substance
Adsorption piece.
(4) after cleaning, magnetic field generating means are closed.It is contained in impeller 400 in container 322, to push cunning
Moving part 300 and fixed adsorption piece.Sliding part 300 moves under the drive of impeller 400 to the direction close to second end 113, sliding
The plugging film 220 and separation membrane 210 of function chamber 150 where dynamic portion 310 successively rupture under the influence of air pressure, and waste liquid is from row
Fluid apertures 160 flows into waste liquid cabin 600, and sliding part 300 continues to move, and the adsorption piece for carrying target substance is made to enter next function
In chamber 150, stop motion moves impeller 400 without being capable of fixing adsorption piece to the direction close to first end 111.It opens
Magnetic field generating means are to provide magnetic field to cylinder 100, so that adsorption piece and cleaning solution mix and clean the suction for carrying target substance
Attachment.
(5) after cleaning, magnetic field generating means are closed.Make impeller 400 to close to second end 113 direction move and
It is partially housed in container 322, to push sliding part 300 and fixed adsorption piece.Drive of the sliding part 300 in impeller 400
The lower direction to close to second end 113 moves, the plugging film 220 and separation membrane 210 of the function chamber 150 where sliding part 310
It successively ruptures under the influence of air pressure, waste liquid flows into waste liquid cabin 600 from drainage hole 160, and sliding part 300 continues to move, and makes to take
Adsorption piece with target substance enters in end chambers 140, stop motion, makes impeller 400 to the direction close to first end 111
Movement is without being capable of fixing adsorption piece.Magnetic field generating means are opened to provide magnetic field to cylinder 100, so that carrying the suction of target substance
Attachment and eluent mix, so that target substance is isolated to pure target substance from adsorption piece.
(6) after eluting, magnetic field generating means are closed.Outlet 190 is connected to detection device.Make impeller 400
It moves and is partially housed in container 322 to the direction close to second end 113, to push sliding part 300 and fixed adsorption piece.
Sliding part 300 moves under the drive of impeller 400 to the direction close to second end 113.Work of the target substance in sliding part 300
It is qualitatively and quantitatively detected in lower inflow detection device.
Said detecting system at least has the advantages that
In the separator 10 of said detecting system, cylinder 100 is separated into end chambers by multiple separation membranes 210
140 and N number of function chamber 150, and multiple separation membranes 210 are tightly connected with cylinder 100, on the side wall of N number of function chamber 150
Drainage hole 160 is offered, each drainage hole 160, which blocks, plugging film 220, can be in the function near first end 111
The adsorption piece for being used for adsorbed target substance is placed in chamber 150, it being capable of placing response liquid or cleaning in other function chamber 150
Liquid etc.；It since sliding part 300 is arranged close to first end 111, and can be slided to the direction close to second end 113, when needs point
When from target substance, sliding part 300 can be contained in the function chamber 150 of first end, it can be in sliding part 300
Adsorption piece is arranged in side far from first end 111, and sample to be separated is added and is adsorbed in target substance on adsorption piece, makes to slide
Moving part 300 is slided to the direction close to second end 113, so that plugging film 220 and separation membrane 210 rupture, and is carried and is adsorbed with mesh
The adsorption piece of mark substance enters other function chamber 150 and is reacted or cleaned, and eventually enters into end chambers 140 and obtains pure
Target substance；When needing to detect target substance, pure target substance can be flowed into detection device from end chambers 140
Detected, can also directly be detected in end chambers 140, can also in end chambers 140 placing response reagent
Or detection reagent, so that target substance is reacted with reaction reagent or detection reagent, to detect target substance.Above-mentioned separator
10 is simple, and the separation and detection of target substance can be realized without uncapping repeatedly, easy to operate, of reduced contamination to sample to be tested.
Above-mentioned separator 10 can be applied in nucleic acid extraction, chemiluminescence detection or cell sorting.
It is appreciated that separation membrane 210 is not limited to three, or more, such as four or five etc..
It is appreciated that waste liquid cabin 600 is not limited to above-mentioned point out shape, or annular hollow structure.When waste liquid cabin 600
When for annular hollow structure, cylinder 100 is at least partially housed in waste liquid cabin 600, and is tightly connected with waste liquid cabin 600.It can be with
Understand, waste liquid cabin 600 also can be omitted.When waste liquid cabin 600 is omitted, the waste liquid that drainage hole 160 flows out can flow directly into outer
In the liquid waste collector in portion.
It is appreciated that detection system is not limited to PCR detection system, it can also be other detection systems, such as: please join together
Fig. 4 is read, in other embodiments, detection system is chemiluminescence detection system, including separator 10 ' and chemiluminescence are examined
Survey device (not shown).
Separator 10 ' is roughly the same with the structure of separator 10, the difference is that: end chambers 140 ' are detection
Chamber.Function chamber 150 ' near first end 111 ' is sample cavity.At least one of remaining function chamber 150 ' is anti-
Answer chamber.
In the illustrated embodiment, separation membrane 210 ' is four.Four separation membranes 210 ' are from second end 113 ' to first end
111 ' are alternatively arranged so that cylinder 100 ' is divided into end chambers 140 ' and four function chambers 150 '.End chambers 140 ' are for holding
Set detection reagent.Function chamber 150 ' near first end 111 ' is sample cavity.From first end 111 ' to close to second end
113 ' direction, excess-three function chamber 150 ' are orderly used to accommodating cleaning solution, reaction reagent and cleaning solution.Reaction reagent is used
In the reaction product reacted with the target substance in sample to be tested, reaction product can be specifically bound with detection reagent and
Issue signal.Wherein, signal is luminous signal.
Chemiluminescence detecting is connect with separator 10 ', to detect to the target substance in sample to be tested.?
In illustrated embodiment, chemiluminescence detecting is light absorption and analytical equipment.Chemiluminescence detecting and end chambers
140 ' connections, and can receive and analyze the luminous signal in end chambers 140 ', with to the target substance in sample to be tested into
Row qualitatively and quantitatively detects.It should be noted that luminous signal can be fluorescence signal, or other signals.
The operation of the chemiluminescence detection system of above embodiment is as follows:
(1) during preparative separation device 10 ', sliding part 310 ' is contained in the function near first end 111 '
In chamber 150 ', and make sliding part 310 ' between adding mouth 170 ' and air equalizer opening 144 ', near first end
Adsorption piece is added in 111 ' function chamber 150 ', and adsorption piece is made to be located at the side of the separate mounting portion 320 ' of sliding part 310 ',
From first end 111 ' to the direction close to second end 113 ', excess-three function chamber 150 ' sequentially adds cleaning solution, is incubated for examination
Agent and cleaning solution.To addition detection reagent in end chambers 140 '.It should be noted that if needing that sample to be tested is cracked and made
When target substance release can just be adsorbed in adsorption piece, lysate is added in the function chamber 150 ' near first end 111 '.
(2) it is added in the function chamber 150 ' of first end 111 ' from adding mouth 170 ' by sample to be tested, opens magnetic
Field generating mechanism is to provide magnetic field to cylinder 100 ', so that sample to be tested and adsorption piece mix.It is to be measured if addition has lysate
Sample is decomposed under the action of lysate and discharges target substance, and the target substance of release is adsorbed with adsorption piece and carried
The adsorption piece of target substance.
(3) after mixing, magnetic field generating means are closed.It is contained in impeller 400 ' in container 322 ', to push
Sliding part 300 ' and fixed adsorption piece.Sliding part 300 ' is transported under the drive of impeller 400 ' to the direction close to second end 113 '
Dynamic, the plugging film 220 ' and separation membrane 210 ' of the function chamber 150 ' where sliding part 310 ' are successively broken under the influence of air pressure
It splits, waste liquid flows into waste liquid cabin 600 ' from drainage hole 170 ', and sliding part 300 ' continues to move, and makes the adsorption piece for carrying target substance
Into in next function chamber 150 ', stop motion, make impeller 400 ' to close to first end 111 ' direction move without
Fixed adsorption piece.Magnetic field generating means are opened to provide magnetic field to cylinder 100 ', so that adsorption piece and cleaning solution are mixed and cleaned
Carry the adsorption piece of target substance.
(4) after cleaning, magnetic field generating means are closed.It is contained in impeller 400 ' in container 322, to push cunning
Moving part 300 ' and fixed adsorption piece.Sliding part 300 ' is transported under the drive of impeller 400 ' to the direction close to second end 113 '
Dynamic, the plugging film 220 ' and separation membrane 210 ' of the function chamber 150 ' where sliding part 310 ' are successively broken under the influence of air pressure
It splits, waste liquid flows into waste liquid cabin 600 ' from drainage hole 170 ', and sliding part 300 ' continues to move, and makes the adsorption piece for carrying target substance
Into in next function chamber 150 ', stop motion, make impeller 400 ' to close to first end 111 ' direction move without
Fixed adsorption piece.Magnetic field generating means are opened to provide magnetic field to cylinder 100 ', so that adsorption piece and reaction reagent mix, are obtained
Carry the adsorption piece of reaction product.
(5) after reaction, magnetic field generating means are closed.It is contained in impeller 400 ' in container 322, to push cunning
Moving part 300 ' and fixed adsorption piece.Sliding part 300 ' is transported under the drive of impeller 400 ' to the direction close to second end 113 '
Dynamic, the plugging film 220 ' and separation membrane 210 ' of the function chamber 150 ' where sliding part 310 ' are successively broken under the influence of air pressure
It splits, waste liquid flows into waste liquid cabin 600 ' from drainage hole 170 ', and sliding part 300 ' continues to move, and makes the adsorption piece for carrying target substance
Into in next function chamber 150 ', stop motion, make impeller 400 ' to close to first end 111 ' direction move without
Fixed adsorption piece.Magnetic field generating means are opened to provide magnetic field to cylinder 100 ', so that adsorption piece and cleaning solution are mixed and cleaned
Carry the adsorption piece of reaction product.
(6) after cleaning, magnetic field generating means are closed.Move impeller 400 ' to the direction close to second end 113 '
And be partially housed in container 322 ', to push sliding part 300 ' and fixed adsorption piece.Sliding part 300 ' is in impeller 400 '
Drive under move to close to the direction of second end 113 ', the plugging film 220 ' of the function chamber 150 ' where sliding part 310 ' with
Separation membrane 210 ' successively ruptures under the influence of air pressure, and waste liquid flows into waste liquid cabin 600 ' from drainage hole 170 ', sliding part 300 '
Continue to move, enter the adsorption piece for carrying reaction product in end chambers 140 ', stop motion makes impeller 400 ' to close
The direction of first end 111 ' moves and is not fixed adsorption piece.Open chemiluminescence detecting.Magnetic field generating means are opened to give
Cylinder 100 ' provides magnetic field, so that the adsorption piece for carrying reaction product is mixed and reacted with detection reagent.Meanwhile chemiluminescence is examined
It surveys device to collect and analyze the adsorption piece for carrying reaction product and the luminous signal in detection reagent reaction process, to treat test sample
Target substance in product is qualitatively and quantitatively analyzed.
The chemiluminescence detection system of above embodiment at least has the advantages that
Structure by the separator 10 ' in above-mentioned chemiluminescence detection system is simple, can be real without uncapping repeatedly
The separation of existing target substance, it is easy to operate, it is of reduced contamination to sample to be tested, meanwhile, it is examined using above-mentioned chemiluminescence detection system
It can directly be detected during surveying target substance, without taking out target substance from separator 10 ', further be subtracted
Few pollution to target substance, guarantees the accuracy of detection.
If the target substance being appreciated that in sample to be tested can directly be specifically bound with detection reagent, reaction
Reagent can be omitted.It can also be more it is appreciated that separation membrane 210 ' is not limited to four, such as five or six etc.,
It can be configured according to the actual situation.
It is appreciated that outlet 190 ' can be omitted.
It is appreciated that detection system is not limited to PCR detection system and chemiluminescence detection system, it can also be other detections
System, such as: in other embodiments, detection system is electrochemical detection system (not shown), including separator and electricity
Chemical detection devices.Separator is roughly the same with the structure of separator 10, the difference is that:
Adsorption piece is chip.Adsorption piece is contained in the function chamber of first end, and is fixed on sliding part.It pushes away
Moving part can be made of non-magnetic material.Magnetic field generating means are omitted.Electrochemical detection device can be to the electricity of end chambers
Signal is collected and analyzes, qualitatively and quantitatively to be analyzed the target substance in sample to be tested.
Structure by the separator in above-mentioned electrochemical detection system is simple, and target can be realized without uncapping repeatedly
The separation and detection of substance, it is easy to operate, it is of reduced contamination to sample to be tested, to guarantee the purity of target substance.
It is appreciated that above-mentioned separator 10 is not limited to use in detection system or electrochemical detection system, can also use
In other separation systems, such as: the cell sorting system (not shown) of an embodiment, including separator and cell detection
Device.The separator of cell sorting system is substantially the same with separator 10, the difference is that:
Cleaning solution in function chamber is that can clean target cell and guarantee the active cleaning solution of target cell or divide
Chaotropic.End chambers are used to that buffer to be housed, with the target cell that suspends.
Wherein, adsorption piece is biomagnetic beads.Magnetic bead can be specifically bound with the recognition site of cell surface to be separated.Tool
Body, magnetic bead is MACS MicroBeads magnetic bead or Dynabeads magnetic bead.Target cell in cell to be separated is hybridoma
Cell or CAR-T cell.It should be noted that adsorption piece may be the chip that can be specifically bound with target cell.When
When adsorption piece is chip, adsorption piece is contained in the function chamber of first end, and is fixed on sliding part.
Cell detection device is connect with separator, to carry out quantitative or qualitative point to the target cell in cell to be separated
From.Further, cell detection device is connect with end chambers, qualitatively or quantitatively to be examined to target cell in end chambers
It surveys.Further, cell detection device can concentration, purity and type etc. to target cell be detected and analyzed.
Wherein, cell detection device is spectrophotometer, Raman spectrometer or chemiluminescence detector.It needs to illustrate
Be, cell detection device be not limited to it is above-mentioned point out device, as long as can to target cell carry out quantitative and qualitative detection device it is equal
It can be used as cell detection device, can be configured according to the actual situation.
Structure by the separator in above-mentioned cell sorting system is simple, and without uncapping repeatedly, that target can be realized is thin
The separation and detection of born of the same parents, it is easy to operate, it is of reduced contamination to sample to be tested, to guarantee the purity of target cell.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (18)
1. a kind of separator characterized by comprising
Cylinder has opposite first end and second end；
Diaphragm assembly, including multiple separation membranes and multiple plugging films, multiple separation membranes be contained in the cylinder and with
The cylinder is tightly connected, and multiple separation membranes are alternatively arranged from the second end to the first end and divide the cylinder
End chambers and N number of function chamber are divided into, the end chambers are in the second end, the side wall of N number of function chamber
Drainage hole is offered, each drainage hole, which blocks, the plugging film；And
Sliding part is arranged close to the first end, and can slide to the direction close to the second end.
2. separator according to claim 1, which is characterized in that the separator further includes impeller, described to push away
Moving part and the sliding part are detachably connected.
3. separator according to claim 2, which is characterized in that further include adsorption piece, the adsorption piece is contained in most
In the function chamber of the first end, the impeller at least partly has magnetism, the impeller and the cunning
The impeller can adsorb the adsorption piece when moving part connection, and the adsorption piece is enable to be adsorbed on the sliding part.
4. separator according to claim 3, which is characterized in that the separator further includes for the absorption
Part provides the magnetic field generating means in magnetic field, and the adsorption piece can be movable under the action of the magnetic field.
5. separator according to claim 3, which is characterized in that the adsorption piece is magnetic bead.
6. separator according to claim 5, which is characterized in that the magnetic bead is superparamagnetism silica nanometer magnetic bead
Or carboxylated magnetic bead.
7. separator according to claim 1, which is characterized in that further include adsorption piece, the adsorption piece is chip, institute
It states adsorption piece to be contained in the function chamber of the first end, and is fixed on the sliding part.
8. separator according to claim 1, which is characterized in that the tensile strength of the separation membrane is greater than the closure
The tensile strength of film, and when sliding part is slided to the direction close to the second end, can make the institute where the sliding part
The indoor pressure of functional chamber is stated to increase, and make the function chamber where the sliding part the plugging film and the separation
Film can successively rupture.
9. separator according to claim 1, which is characterized in that it further include waste liquid cabin, the waste liquid cabin and the row
Fluid apertures connection.
10. separator according to claim 9, which is characterized in that the waste liquid cabin is annular hollow structure, the cylinder
Body is at least partially housed in the waste liquid cabin, and is tightly connected with the waste liquid cabin；
Or, the waste liquid cabin is tubular, the outer wall of the outer wall and the cylinder in the waste liquid cabin is affixed.
11. separator according to claim 9, which is characterized in that the side wall of the cylinder is also provided with to give up with described
The air equalizer opening that liquid tank communicates, the air equalizer opening are arranged close to the first end.
12. separator according to claim 1, which is characterized in that the cylinder offers adding mouth, the adding mouth
It is located most closely in the cavity wall of the function chamber of the first end, the separator further includes for sealing the sample-adding
The sealing element of mouth.
13. the described in any item separators of claim 1~12 are in nucleic acid extraction, chemiluminescence detection or cell sorting
Using.
14. a kind of detection system, which is characterized in that including claim 1~6 and 8~12 described in any item separators.
15. detection system according to claim 14, which is characterized in that the detection system is PCR detection system, described
End chambers are test chamber, and the function chamber near the first end is sample cavity, in remaining described function chamber
At least one for cleaning chamber；
Or, the detection system is chemiluminescence detection system, the end chambers are test chamber, near the first end
The function chamber is sample cavity, and at least one of remaining described function chamber is reaction chamber.
16. a kind of electrochemical detection system, which is characterized in that including claim 1~2 and 7~12 described in any item separation
Device.
17. electrochemical detection system according to claim 16, which is characterized in that the end chambers are test chamber, most
The function chamber close to the first end is sample cavity, and at least one of remaining described function chamber is cleaning chamber.
18. a kind of cell sorting system, which is characterized in that including the described in any item separators of claim 1~12.
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| CN201811169486.6A CN109486651B (en) | 2018-10-08 | 2018-10-08 | Separation device and application thereof, detection system, electrochemical detection system and cell sorting system |
| PCT/CN2019/108677 WO2020073829A1 (en) | 2018-10-08 | 2019-09-27 | Separation device and use thereof, detection system, electrochemical detection system and cell sorting system |
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| CN201811169486.6A CN109486651B (en) | 2018-10-08 | 2018-10-08 | Separation device and application thereof, detection system, electrochemical detection system and cell sorting system |
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| WO2020073829A1 (en) * | 2018-10-08 | 2020-04-16 | 广东菲鹏生物有限公司 | Separation device and use thereof, detection system, electrochemical detection system and cell sorting system |
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