CN109472748A - The brightness of image bearing calibration extracted based on nano particle SEM image brightness - Google Patents

The brightness of image bearing calibration extracted based on nano particle SEM image brightness Download PDF

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CN109472748A
CN109472748A CN201811257701.8A CN201811257701A CN109472748A CN 109472748 A CN109472748 A CN 109472748A CN 201811257701 A CN201811257701 A CN 201811257701A CN 109472748 A CN109472748 A CN 109472748A
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brightness
nano particle
sem image
matrix
image
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CN109472748B (en
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赵红东
李宇海
孙梅
H.S.艾哈迈德
王储
赵泽通
张洁
闫苗
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Hebei University of Technology
CETC 53 Research Institute
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Hebei University of Technology
CETC 53 Research Institute
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Abstract

The present invention is based on the brightness of image bearing calibrations that nano particle SEM image brightness is extracted, it is related to general image real time transfer, this method is to carry out whole brightness based on nano particle SEM image to extract and then be corrected the uneven brightness of the image whole region, there is high brightness in some boundary part region especially in nano particle SEM image, and in the case where there is darker brightness in its another boundary part region, show the average brightness of each pixel of nano particle SEM image, and realize the whole region brightness uniformity of nano particle SEM image, it overcomes since high brightness and darker brightness often respectively appear in image difference peripheral boundary in nano particle SEM image, the prior art then cannot achieve nano particle SEM image peripheral limit part and carry out interpolation, cause gained The defect of the brightness irregularities of the nano particle SEM image arrived.

Description

The brightness of image bearing calibration extracted based on nano particle SEM image brightness
Technical field
Technical solution of the present invention is related to general image real time transfer, is specifically based on nano particle SEM image The brightness of image bearing calibration that brightness is extracted.
Background technique
In scanning electron microscope (English is Scanning Electron Microscope, below abbreviation SEM) Electron beam accelerates through overvoltage, is acted on by magnetic lenses convergence and scanning coil, observes sample using secondary electron signal imaging The configuration of surface of product has reached nanoscale ultrahigh resolution, is widely used in medicine, biology and material subjects Microcosmic research field.
Cause filament to deviate and swing since Electronic Speculum works long hours, caused by the non-uniform electric current of reflection, and loading The insecure generation low dip of platform and cause sample reception emission current inconsistent, will appear brightness in the SEM image obtained at present Unevenly, some region high brightness in nano particle SEM image, some region low-light levels affect nano particle SEM image Normal display.To solve the above-mentioned problems, those skilled in the art have carried out a series of researchs.CN100411445C discloses school The image processing method and device of positive image Luminance Distribution, which describe extracted from the image data of image low-frequency brightness at Point, using the product and operation with multiple Gaussian functions, luminance components distribution is found out, detects the characteristic quantity school of brightness of image ingredient The actual luminance components of positive image, however, finding out brightness under multiple degree since Gaussian function has certain distribution The region of component distributing is less than original image.CN101655976B is disclosed based on the modified multi-view image brightness in control point Bearing calibration, this method count brightness histogram using two width multi-view images of input, and still, SEM equipment is only to provide one A directional image.CN104778664A discloses a kind of method of brightness of image correction, and this method is by image dividing regions to be corrected As current region to be corrected behind domain, the correction system of each pixel in current region to be corrected is determined using bilinear interpolation algorithm Number, original luminance value and the correction coefficient by each pixel in the current region to be corrected, determines each pixel school Brightness value after just, and the region to be corrected is shunk to the optical centre of image, the region after contraction is divided work again To be current to be corrected, until reaching default number of corrections, this method is there are more number of corrections, and bilinear interpolation Algorithm can only realize its internal interpolation, and correcting area is less than the defect of original image.CN107590787A discloses a kind of scanning The image distortion correction method of electron microscope is the image distortion correction method for scanning electron microscope, and utilizes Shooting standard target obtains sparse image pixel location information and corresponding Distortion Vector sample set, exists and needs multiple image Defect.
In short, the defect of the existing technology of above-mentioned brightness of image correction is: due to being highlighted in nano particle SEM image Degree often respectively appears in the different peripheral boundaries of image with darker brightness, and the prior art then cannot achieve image peripheral boundary part Interpolation is carried out, the brightness irregularities of obtained nano particle SEM image are caused.
Summary of the invention
The technical problems to be solved by the present invention are: providing the brightness of image extracted based on nano particle SEM image brightness Bearing calibration, this method are to carry out whole brightness based on nano particle SEM image to extract and then to the image whole region Uneven brightness is corrected, high brightness occurs in some boundary part region especially in nano particle SEM image, and In the case that darker brightness occurs in its another boundary part region, each pixel of nano particle SEM image is shown Average brightness, and realize the whole region brightness uniformity of nano particle SEM image, it overcomes due to nano particle SEM image Middle high brightness and darker brightness often respectively appear in image difference peripheral boundary, and the prior art then cannot achieve nano particle SEM image peripheral limit part carries out interpolation, causes the defect of the brightness irregularities of obtained nano particle SEM image.
The present invention solves technical solution used by the technical problem: being extracted based on nano particle SEM image brightness Brightness of image bearing calibration is a kind of to carry out whole brightness based on nano particle SEM image and extract and then to the complete of the image The corrected method of portion region uneven brightness, the specific steps are as follows:
The nano particle SEM image of brightness irregularities is divided into M × N number of piecemeal by the first step:
Nano particle SEM image is indicated with P, and the nano particle SEM image of wherein brightness irregularities is divided into longitudinal M etc. Part and transverse direction N equal portions, total M × N number of piecemeal do not overlap each other between piecemeal and piecemeal other than adjacent block boundary, each In piecemeal, longitudinally there is m pixel, laterally there is n-pixel, m and n are even number, longitudinal M of corresponding entire nano particle SEM image P × M pixel and lateral N × n-pixel, by the corresponding actual range of pixel, to calculate nano particle SEM image P and each point The actual size of block, the quantity that pixel is covered in single segmented areas is >=10 nano particles, and brightness irregularities are received The vertical and horizontal of rice grain SEM image include at least 3 segmented areas;
Second step calculates the average brightness and whole image average brightness of each segmented areas image:
To the block image that the nano particle SEM image of wherein brightness irregularities is divided by the above-mentioned first step, calculate every The average brightness of a segmented areas image is the brightness of piecemeal central point, and assigns matrix expression all piecemeal average brightness, Concrete operations are as follows:
The average brightness a of (I, J) a piecemeal of longitudinal label I and lateral label JI,JIt is calculated with formula (1),
In formula (1), Pu,vIndicate longitudinal u-th of pixel and lateral v in the nano particle SEM image of brightness irregularities The brightness of one point of a pixel, longitudinal label I=1,2 ..., M;Lateral label J=1,2 ... .., N;
With average brightness aI,JThe average brightness of all piecemeals is constituted into nano particle SEM image piecemeal according to the position of block Average brightness matrixAs shown in formula (2),
In formula (2), M is longitudinal equal portions of the nano particle SEM image piecemeal of brightness irregularities, and N is brightness irregularities Nano particle SEM image piecemeal lateral equal portions, the nano particle SEM image piecemeal average brightness matrix of brightness irregularities Matrix element aI,JThe central point of each piecemeal of the non-uniform nano particle SEM image of corresponding brightness is AI,J, wherein Zong Xiangbiao Number I=1,2 ..., M;Lateral label J=1,2 ..., N;Thus the calculating of the average brightness of each segmented areas image is completed;
The whole image average brightness of the nano particle SEM image of brightness irregularities is calculated using formula (3)
In formula (3), M is longitudinal equal portions of the nano particle SEM image piecemeal of brightness irregularities, and N is brightness irregularities Nano particle SEM image piecemeal lateral equal portions, aI,JFor average brightness, longitudinal label I=1,2 ..., M;Lateral label J= 1,2 ... .., N;
Thus the calculating of the average brightness and whole image average brightness of each segmented areas image is completed;
Third step obtains linear extrapolation by piecemeal average brightness matrix and expands luminance matrix:
To the nano particle SEM image piecemeal average brightness matrix for the brightness irregularities that above-mentioned second step obtainsCarry out line Property extrapolation expand, obtain linear extrapolation expand luminance matrixAs expand the matrix of M+2 row N+2 columnConcrete operations are as follows:
Firstly, order matrixThe first row, last line, first row and last column matrix element be 0, intermediate matrix Member by with matrixComposition, matrixWith matrixBetween meet relationship shown in following formula (4),
For this purpose, expanding matrixFor shown in following formula (5),
Expand matrixFor shown in following formula (6),
Secondly, expanding method according to linear extrapolation, above-mentioned expansion matrix is modifiedThe first row and last line matrix element Following formula (7) and formula (8),
b1,J=2 × b2,J-b3,J(7),
bM+2,J=2 × bM+1,J-bM,J(8),
In formula (7) and formula (8), lateral label J=2,3 ..., N+1;
Equally, above-mentioned expansion matrix is modifiedFirst row and last column following formula of matrix element (9) and formula (10),
bI,1=2 × bI,2-bI,3(9),
bI,N+2=2 × bI,N+1-bI,N(10),
In formula (9) and formula (10), longitudinal label I=2,3 ..., M+1;
For above-mentioned expansion matrixMatrix element b on four angles1,1、b1,N+2、bM+2,1And bM+2,N+2Respectively following formula (11), formula (12), formula (13) and formula (14),
Linear extrapolation is obtained as a result, expands luminance matrixShown in following formula (15),
In formula (15), brightness b1,JCorresponding spatial point BI,JBrightness, spatial point BI,JCoordinate be laterally (J-1/2) × n + 1 pixel and longitudinal (I-1/2) × m+1 pixel, here, lateral label J=1,2 ..., N+2;Longitudinal label I=1,2 ..., M+ 2;
Thus it completes to obtain linear extrapolation expansion luminance matrix by piecemeal average brightness matrix;
4th step extracts the brightness of each point in the nano particle SEM image of brightness irregularities:
(4.1) step determines each brightness in four surrounded regions of point:
Matrix is expanded by linear extrapolation obtained in above-mentioned third stepIn neighbouring four matrix element bI,J、bI,J+1、 bI+1,J+1And bI+1,J, corresponding rectangular BI,JBI,J+1BI+1,J+1BI+1,JFour point BI,J、BI,J+1、BI+1,J+1And BI+1,JIt is right Brightness is answered, the linear extrapolation is calculated with formula (16) and expands matrixIn neighbouring corresponding four points of four matrix elements BI,JBI,J+1BI+1,J+1BI+1,JSurround each point C in rectangular rangeI,J,i,jBrightness cI,J,i,j,
In formula (16), rectangular BI,JBI,J+1BI+1,J+1BI+1,JInterior longitudinal direction ith pixel, i=1,2 ..., m;Rectangular BI, JBI,J+1BI+1,J+1BI+1,JJ-th of pixel of interior transverse direction, j=1,2 ..., n;Longitudinal label I=1,2 ..., M+1;Lateral label J= 1,2 ..., N+1;X is pixel Ci,jApart from side BI,JBI,J+1Vertical range, y be pixel Ci,jApart from side BI,JBI+1,JHang down Straight distance, unit are pixel, and 0≤x≤m, 0≤y≤n;
(4.2) step extracts the brightness of each point in the nano particle SEM image of brightness irregularities:
By matrix element gu,vConstitute the luminance matrix of the nano particle SEM image of brightness irregularities In matrix element gu,vWith pixel Ci,jBrightness ci,jRelationship such as formula (17) shown in,
g(I-1)×m+i-m/2,(J-1)×n+j-n/2=cI,J,i,j(17),
In formula (17), g(I-1)×m+i-m/2,(J-1)×n+j-n/2That is matrix element gu,v, i=1,2 ..., m;J=1,2 ..., n;I =1,2 ..., M+1;J=1,2 ..., N+1;It is constrained to 1≤[(I-1) × m+i-m/2]≤M × m and 1≤[(J-1) × n+j-n/ 2]≤N × n, u=(I-1) × m+i-m/2, v=(J-1) × n+j-n/2;
By matrix element guvThe luminance matrix of the nano particle SEM image of the brightness irregularities of compositionFollowing formula (18) institute Show,
In formula (18), longitudinal label u=1,2 ..., M × m, lateral label v=1,2 ..., N × n;
So far it completes to extract the brightness that each is put in the nano particle SEM image of brightness irregularities;
5th step calculates every bit brightness correction coefficients, realizes the correction of nano particle SEM image uneven brightness:
The whole image average brightness of the nano particle SEM image of brightness irregularities is calculated with formula (3)On the basis of, meter Calculate the correction coefficient of each pointAnd each of the nano particle SEM image of brightness irregularities is realized with formula (19) The gamma correction q of pointu,v,
In formula (19), Pu,vIndicate longitudinal u-th of pixel and lateral v in the nano particle SEM image of brightness irregularities The brightness of one point of a pixel, u=1,2 ..., M × m;V=1,2 ..., N × n;For non-uniform nano particle SEM figure The whole image average brightness of picture,
As shown in formula (20), the gamma correction g of each point of the nano particle SEM image of brightness irregularitiesu,vIt constitutes The matrix of the nano particle SEM gamma correction image of brightness irregularities
The matrix of the nano particle SEM gamma correction image of brightness irregularitiesShow that nano particle SEM image is uneven The effect of the correction of brightness;
So far it completes to calculate every bit brightness correction coefficients, realizes the correction of nano particle SEM image uneven brightness.
The above-mentioned brightness of image bearing calibration extracted based on nano particle SEM image brightness, it is described will wherein brightness disproportionation Even nano particle SEM image is divided into longitudinal M equal portions and transverse direction N equal portions, wherein M=4, N=6.
The above-mentioned brightness of image bearing calibration extracted based on nano particle SEM image brightness in each described piecemeal, is indulged To there is m pixel, laterally there is n-pixel, wherein n=100 pixel, m=100 pixel.
The above-mentioned brightness of image bearing calibration extracted based on nano particle SEM image brightness, the linear interpolation method are this The known method of technical field, related operation and calculating are that those skilled in the art will appreciate that.
The beneficial effects of the present invention are: compared with prior art, substantive distinguishing features outstanding of the invention and marked improvement It is as follows:
(1) prior art will find out average brightness by square, then use linear interpolation method, correct brightness of image, only There is the region unit for selecting a certain size, just can reflect its true brightness, direct piecemeal expands luminance matrix without extrapolating, only The brightness of image in the point range of block center can be obtained, and the present invention uses and obtains linear extrapolation expansion by piecemeal average brightness matrix Luminance matrix contains nano particle SEM image to expand the region that linear extrapolation exhibition luminance matrix corresponding blocks central point surrounds Each point.
(2) brightness is averaging to arbitrary image piecemeal in the prior art, does not point out the requirement of piecemeal, and the present invention is true It surely will be according to average brightness not be influenced substantially by individual particle in segmented areas, it is indicated that the constraint condition of piecemeal size.
(3) prior art can not achieve the brightness of image of whole region in the application of facial image piecemeal correcting luminance Correction, and the present invention applies the brightness of image correction in nano particle SEM image all areas.
(4) prior art is not described number of blocks in application block correction brightness of image, and divides in the present invention Transverse direction and longitudinal direction respectively at least divides 3 piecemeals, obtains linear extrapolation and expands luminance matrix, is extracted receiving for brightness irregularities The brightness of each point in rice grain SEM image.
(5) the multiple stepping bearing calibration of small step-length is used in the prior art, and the present invention is expanded using piecemeal luminance matrix The method that brightness once corrects nano particle SEM image improves correction efficiency;
(6) in a kind of method of brightness of image of prior art CN104778664A correction by the region to be corrected to image Optical centre shrink, the correction coefficient of each pixel in current region to be corrected is determined using linear interpolation algorithm, passes through institute The original luminance value of each pixel and the correction coefficient in current region to be corrected are stated, the brightness after determining each pixel correction Value, and the region to be corrected is shunk to the optical centre of image, the region after contraction is divided again as currently to school Just, until reaching default number of corrections, correction is there are more number of corrections in the prior art, and bilinear interpolation Algorithm can only realize its internal interpolation, and correcting area is less than original image, and without being related to extrapolating, therefore cannot school Positive image edge portions.And the present invention only need once to divide, spread to interpolation outside just and realize it is bright based on nano particle SEM image The brightness of image correction that degree extracts.
(7) prior art CN100411445C corrects the image processing method of image brightness distribution and device is from image Low-frequency brightness ingredient is extracted in image data, detects the characteristic quantity of brightness of image ingredient, with low-frequency brightness ingredient and parameter The actual luminance components of above-mentioned image are corrected, wherein luminance components distribution is found out using product and operation with multiple Gaussian functions, Since Gaussian function has certain size, finds out the region that luminance components are distributed under multiple degree and be less than original image. And then the present invention is obtained every in nano particle SEM image using linear extrapolation around Block Brightness matrix using linear interpolation method One brightness is realized and is corrected in entire nano material SEM image whole region.
(8) CN101655976B is more using two width of input based on the modified multi-view image brightness bearing calibration in control point Multi-view image counts brightness histogram, and the directional image that SEM equipment often only provides, the present invention then need to only use nanometer A sub-picture of material SEM.
(9) a kind of image distortion correction method of scanning electron microscope of CN107590787A is for scanning electron microscopy The image distortion correction method of mirror, and sparse image pixel location information and corresponding distortion are obtained using shooting standard target Vector sample set needs multiple image, and the present invention corrects and only needs a sub-picture in the brightness of nano material SEM image, is not required to It shoots standard target and obtains sparse image pixel positions.
(10) it extrapolates present invention uses piecemeal brightness linear and expands matrix, it is each then to extract nano particle SEM image The brightness of point, the Integral correction brightness of nano particle SEM image avoid the mutation after correction between image block, overcome It is eliminated using gradient method and is mutated bring arbitrariness between block.
(11) present invention using piecemeal luminance matrix in being expanded, and only piecemeal luminance matrix is increased, and exploded view As the central point of block, and the storage of nano particle SEM image does not increase, and piecemeal luminance matrix is far smaller than nano particle SEM image homography, has saved memory space.
(12) present invention carries out homogenization brightness using nano particle SEM image, and same method can be generalized to medicine SEM image and optical microscope image have versatility.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the operating process schematic block diagram of the method for the present invention.
Fig. 2 is nano particle SEM image and divided block area schematic in the method for the present invention.
Fig. 3 is that the nano particle SEM image in the method for the present invention divides M × N number of piecemeal and the central point of each piecemeal shows It is intended to.
Fig. 4 is that the nano particle SEM image in the method for the present invention divides the corresponding luminance matrix expression shape of M × N number of piecemeal Formula schematic diagram.
Fig. 5 is the center of the nano particle SEM image brightness linear extrapolation expansion matrix in the method for the present invention and corresponding blocks Point schematic diagram.
Fig. 6 illustrates to supplement the expansion of 0 matrix element around the nano particle SEM image luminance matrix in the method for the present invention Figure.
Fig. 7 is that matrix schematic diagram is expanded in the nano particle SEM image brightness linear extrapolation in the method for the present invention.
Fig. 8 is that the expansion point of upper left three in matrix corresponding blocks is expanded in the nano particle SEM image brightness in the method for the present invention The area schematic surrounded with nano particle SEM image upper left piecemeal average brightness point.
Fig. 9 is corresponding for any neighbour's matrix element in the nano particle SEM image brightness expansion matrix in the method for the present invention The square region schematic diagram that block central point surrounds.
Figure 10 is the nano particle SEM image schematic diagram used in the embodiment of the present invention 1.
Figure 11 is that the image of each piecemeal average brightness of nano particle SEM image in the embodiment of the present invention 1 shows signal Figure.
Figure 12 is to carry out linear difference to viewing area brightness using 4 neighbour's differential techniques in the embodiment of the present invention 1, is obtained Obtain the image display schematic diagram of whole image brightness.
Figure 13 is the image schematic diagram in 1 nano particle SEM image whole region of the embodiment of the present invention after gamma correction.
In figure, 201. nano particle SEM image peripheral boundaries, 501. expand image block, and 502. nano particle SEM images are opened up The peripheral boundary of block is opened up, the corresponding expansion block of the matrix element of upper left three in matrix-block is expanded in 801. nano particle SEM image brightness Square is expanded in the region that central point and nano particle SEM image upper left piecemeal central point surround, 901. nano particle SEM image brightness Neighbouring four matrix element b in battle arrayI,J、bI,J+1、bI+1,J+1And bI+1,JCorresponding blocks central point BI,J、BI,J+1、BI+1,J+1And BI+1,JIt surrounds Square region,
In figure, the pixel that image as unit uses, drawing image size is directly proportional to pixel quantity.
Specific embodiment
Embodiment illustrated in fig. 1 shows that the operating process of the method for the present invention is: obtaining nano particle SEM image;Not by brightness Uniform nano particle SEM image is divided into M × N number of piecemeal;Calculate the average brightness of each segmented areas image;It is put down by piecemeal Equal luminance matrix obtains linear extrapolation and expands luminance matrix;Extract each point in the nano particle SEM image of brightness irregularities Brightness;Every bit brightness correction coefficients are calculated, realize the correction of nano particle SEM image uneven brightness.
Fig. 2 shows the nano particle SEM image and divided block region to be processed in the method for the present invention, wherein solid line table Show nano particle SEM image peripheral boundary, dotted line is nano particle SEM image divided block region, nano particle SEM image point M × N number of piecemeal, other than adjacent block boundary, phase between block and block have been divided at lateral N equal portions and longitudinal direction M equal portions, whole image It does not overlap.Each piecemeal transverse width is n-pixel and longitudinal direction is highly m pixel, and n is even number, and m is even number.
In Fig. 2, the 1 of abscissa, 2 ... .., N are the number in the lateral divided block region of nano particle SEM image;It is vertical to sit Target 1,2 ... .., M are the number in longitudinally divided piece of region of nano particle SEM image.
Divided by the piecemeal that Fig. 2 illustrates present invention nano particle SEM general image to be processed, with normal image piecemeal and The difference of local block includes at least ten nano particles in piecemeal of the present invention, and the average brightness of multiple particles is not substantially in block It is influenced by single nano particle intrinsic brightness, in normal image and is not had the feature;On the other hand, nano particle SEM schemes Transverse direction and longitudinal direction at least divides three equal portions as in, to guarantee to have enough initial points, realizes that brightness mean matrix is linearly outer Expansion matrix is pushed away, in 100,000 pixel nano particle SEM images, can satisfy the two requirements completely.Fig. 2 shows nano particle The peripheral boundary 201 of SEM image by it is longitudinally divided be 5 equal portions, be laterally divided into 4 equal portions, whole image has divided 5 × 4 points Block.
Fig. 3 is that the nano particle SEM image in the method for the present invention divides M × N number of piecemeal and the central point of each piecemeal shows It is intended to, it is shown that nano particle SEM image divides the central point of M × N number of piecemeal and each piecemeal, and stain indicates nano particle SEM image divided block central point, in the case where block size is appropriate, the average brightness of calculation block, equal to the brightness at block center.Fig. 3 is shown The peripheral boundary 201 of nano particle SEM image by it is longitudinally divided be 5 equal portions, be laterally divided into 4 equal portions, whole image has divided 5 × 4 piecemeals.
In Fig. 3, A1,1、A1,2、....、A1,NIt is respectively the center of the N number of piecemeal of the first row in nano particle SEM image piecemeal Point, A2,1、A2,2、....、A2,NIt is respectively the central point of the N number of piecemeal of the second row in nano particle SEM image piecemeal, AM,1、 AM,2、....、AM,NIt is respectively the central point of the N number of piecemeal of M row in nano particle SEM image piecemeal.AI,JAnd BI,JIn lower footnote First letter I indicates that longitudinal label, second letter J indicate lateral label.
The central point that nano particle SEM image of the present invention divides M × N number of piecemeal and each piecemeal, nanometer are illustrated by Fig. 3 Uneven brightness gradually changes in particle SEM image, and nano particle is far smaller than the size of divided block, and it is appropriate to divide size in block Under, the average brightness of block is equal to the brightness of the block central point, and each piecemeal center point coordinate and its brightness are to realize brightness linear The necessary condition of matrix and linear interpolation is expanded in extrapolation.
Fig. 4 is that the nano particle SEM image in the method for the present invention divides the corresponding luminance matrix expression shape of M × N number of piecemeal Formula is divided into M × N number of piecemeal in nano particle SEM image, calculates the corresponding average brightness of each piecemeal, constitutes M row N column Average brightness matrix.
Nano particle SEM image in the present invention is illustrated by Fig. 4 and divides each piecemeal average brightness of M × N number of piecemeal.Figure In 4, aI,J、bI,JFirst letter I indicates that longitudinal label, second letter J indicate lateral label in lower footnote.aI,JFor transverse direction The average brightness of serial number J and longitudinal serial number I piecemeal, I=1,2 ..., M;J=1,2 ..., N.a1,1It is average in corresponding (1,1) block Brightness is equal to the central point A of the block1,1Brightness, i.e. point A1,1Brightness be a1,1, point A1,2Brightness be a1,2..., point AM,NBrightness be aM,N, the luminance matrix of M × N number of piecemeal average brightness homography M row N column
The average brightness a of calculation blockI,JFor,
In formula (1), Pu,vFor the brightness of nano particle SEM image (u, v) point, by aI,JConstitute nano particle SEM figure As piecemeal average brightness matrixFor,
Fig. 5 shows that the extrapolation of the nano particle SEM image brightness linear in the method for the present invention is expanded in matrix and corresponding blocks Heart point, nano particle SEM image piecemeal obtain the average brightness matrix of M row N column, carry out linear extrapolation to average brightness matrix and open up Matrix is opened up, determines that the brightness linear of M+2 row N+2 column is extrapolated and expands matrix, in the brightness of matrix element corresponding blocks central point, include The corresponding center for expanding block of matrix element of matrix is expanded in the central point of divided block and extrapolation in nano particle SEM real image Point.In Fig. 5, BI,JMatrix element b is expanded for material SEM image brightnessI,JThe central point of corresponding blocks, I=1,2 ..., M+2;J=1, 2 ..., N+2.
The positional relationship that matrix corresponding blocks are expanded in material SEM image brightness linear extrapolation in the present invention is illustrated by Fig. 5, it is bright It spends matrix linear extrapolation to expand during matrix, matrixUp and down respectively increase a line and left and right respectively increase by one column, by M × N matrixIt is expanded into the matrix of (M+2) × (N+2)It is equal to the block center according to the average brightness of luminance matrix member corresponding blocks The brightness of point, therefore luminance matrix expands out two column of two rows up and down and left and right, the nano particle corresponding to nano particle SEM image About 201 SEM image peripheral boundary respectively expands out a line image and left and right respectively expands out a column and expands image block 501, Nano particle SEM image expands the peripheral boundary 502 of block, and in practical nano particle SEM image, these are expanded block and do not deposit Image concrete form is expanded out outward, the present invention is also practical, has only been expanded average brightness matrix element and has been expanded block Central point.
Fig. 6 is the expansion that 0 matrix element is supplemented around the nano particle SEM image luminance matrix in the method for the present invention, is given Out by nano particle SEM image average brightness matrix and the form for forming extrapolation and expanding matrix of surrounding 0 matrix element of supplement.
Material SEM image luminance matrix in the present invention is illustrated by Fig. 6 and expands initial procedure, defines (M+2) × (N+2) MatrixInitial representation, matrixOutermost two rows up and down and two column matrix members of left and right are 0, intermediate M × N number of matrix element with MatrixIt is identical, matrix calculating matrix are expanded according to linear extrapolationOutermost two column matrix of two rows up and down and left and right Member.
Fig. 7 is that matrix is expanded in the SEM image brightness linear extrapolation in the method for the present invention, shows nano particle SEM image Piecemeal average brightness expands the title of each matrix element of matrix.In Fig. 7, bI,JIt is the brightness expansion square of nano particle SEM image Array element, the central point of corresponding blocks are BI,J, I=1,2 ..., M+2;J=1,2 ..., N+2.
SEM image brightness linear extrapolation in the present invention is illustrated by Fig. 7 and expands each position in matrix, in the present invention root Method is expanded according to linear extrapolation, outermost two rows up and down and left and right two column matrix member for expanding matrix B are calculated by formula (7)~(14),
b1,J=2 × b2,J-b3,J(7),
bM+2,J=2 × bM+1,J-bM,J(8),
In formula (7) and formula (8), lateral label J=2,3 ..., N+1;
bI,1=2 × bI,2-bI,3(9),
bI,N+2=2 × bI,N+1-bI,N(10),
In formula (9) and formula (10), longitudinal label I=2,3 ..., M+1;
For above-mentioned expansion matrixMatrix element b on four angles1,1、b1,N+2、bM+2,1And bM+2,N+2Respectively following formula (11), formula (12), formula (13) and formula (14),
It obtains expanding matrix
Fig. 8 shows that the expansion of upper left three in matrix corresponding blocks is expanded in the nano particle SEM image brightness in the method for the present invention The region that point and nano particle SEM image upper left piecemeal average brightness point surround.In Fig. 8, BI,J、BI,J+1、BI+1,J+1And BI+1,J. Neighbouring four matrix element b in matrix are expanded with Block Brightness linear extrapolationI,J、bI,J+1、bI+1,J+1And bI+1,JRespectively correspond rectangular four A point.
It is illustrated by Fig. 8 and expands cashier corresponding to the nano particle SEM image peripheral boundary 201 of nano particle SEM image The corresponding central point for expanding block of the matrix element of upper left three and nano particle SEM figure in matrix-block are expanded in rice grain SEM image brightness Picture.The prior art can not cover whole using only the corresponding piecemeal central point of nano particle SEM image piecemeal average brightness matrix A nano particle peripheral boundary near zone, and the present invention contains brightness linear extrapolation and expands in the corresponding expansion block of matrix Heart point may include each of entire nano particle SEM image pixel.
It is corresponding that Fig. 9 shows that any neighbour's matrix element in matrix is expanded in the nano particle SEM image brightness in the method for the present invention The square region that surrounds of block central point, expand the corresponding squared region arbitrarily surrounded of matrix from nano particle SEM image brightness Domain is able to observe that the positional relationship for needing to calculate luminance point and four Neighbor Points.In Fig. 9, Ci,jFor by BI,J、BI,J+1、 BI+1,J+1And BI+1,JSpatial point in the square region surrounded.BI,J、BI,J+1、BI+1,J+1And BI+1,JTo be opened up with Block Brightness linear extrapolation Open up neighbouring four matrix element b in matrixI,J、bI,J+1、bI+1,J+1And bI+1,JRespectively correspond four rectangular points.
Brightness of image is illustrated by Fig. 9 and expands the corresponding four points B of four points of neighbour in matrixI,J、BI,J+1、BI+1,J+1With BI+1,JPoint C to be corrected in the square region surroundedi,jPositional relationship, point Ci,jIn square region BI,J BI,J+1BI+1,J+1BI+1,J On the inside or side surrounded, four point B of neighbourI,J、BI,J+1、BI+1,J+1And BI+1,JSquare region transverse width m pixel is surrounded, Longitudinal height n-pixel, pixel C to be askedi,jApart from side BI,JBI,J+1Vertical range x pixel, apart from side BI,JBI+1,JIt is vertical Distance y pixel, and 0≤x≤m, 0≤y≤n.In Fig. 9, x is the spatial point C of brightness to be askedi,jApart from side BI,JBI,J+1It is vertical Distance, 0≤x≤m, unit are pixel;Y is the spatial point C of brightness to be askedi,jApart from side BI,JBI+1,JVertical range, 0≤y≤ N, unit are pixel.
Nano particle CI,J,i,jThe brightness c of pointI,J,i,jPass through four point B of neighbourI,J、BI,J+1、BI+1,J+1And BI+1,JPair Answering brightness is bI,J、bI+1,J、bI,J+1And bI+1,J+1It determines,
In formula (16), rectangular BI,JBI,J+1BI+1,J+1BI+1,JInterior longitudinal direction ith pixel i=1,2 ..., m;Rectangular BI, JBI,J+1BI+1,J+1BI+1,JInterior transverse direction j-th pixel j=1,2 ..., n;Longitudinal label I=1,2 ..., M+1;Lateral label J=1, 2 ..., N+1;X indicates pixel Ci,jApart from side BI,JBI,J+1Vertical range, y indicate pixel Ci,jApart from side BI,JBI+1,J's Vertical range, unit are pixel, and 0≤x≤m, 0≤y≤n;
By matrix element guvConstitute the luminance matrix of the nano particle SEM image of brightness irregularities In matrix element gu,v With pixel Ci,jBrightness ci,jRelationship such as formula (17) shown in,
g(I-1)×m+i-m/2,(J-1)×n+j-n/2=cI,J,i,j(17),
In formula (17), g(I-1)×m+i-m/2,(J-1)×n+j-n/2That is matrix element gu,v, i=1,2 ..., m;J=1,2 ..., n;I =1,2 ..., M+1;J=1,2 ..., N+1;It is constrained to 1≤[(I-1) × m+i-m/2]≤M × m and 1≤[(J-1) × n+j-n/ 2]≤N × n, u=(I-1) × m+i-m/2, v=(J-1) × n+j-n/2;
By matrix element gu,vThe luminance matrix of the nano particle SEM image of the brightness irregularities of compositionFollowing formula (18) It is shown,
So far it completes to extract the brightness that each is put in the nano particle SEM image of brightness irregularities;
Figure 10 shows the nano particle SEM image used in the embodiment of the present invention 1, and size is lateral 600 pixels, longitudinal 400 pixels, each pixel is 1 nanometer corresponding, the brightness disproportionation that nano particle SEM image used by showing in figure occurs Even, the obvious brightness in the upper left corner is high, and the lower right corner is darker.Numerous nano particles are contained in nano particle SEM image, since image is bright Degree is uneven, shows that nano particle quantity is more in bright areas, and shows that nano particle is less in brightness darker area, this is to receive Result caused by rice grain SEM image is shown.However, actually nano particle SEM image has recorded nanometer of different sizes Grain, nano particle distribution density is not much different in whole image, therefore the luminance difference that nano particle SEM image is shown influences The correct display of nano particle.
Figure 11 shows the image of each piecemeal average brightness of nano particle SEM image in the embodiment of the present invention 1.This implementation Nano particle SEM image in example is by being laterally divided into 6 equal portions and being divided longitudinally into 4 equal portions, calculation shows that different piecemeals are average bright Degree has differences, and illustrates the average brightness of nano particle SEM image in the present embodiment being divided into 24 piecemeals, shows difference Difference existing for piecemeal, upper left corner piecemeal is brighter, and the lower right corner is darker.
The average brightness a of (I, J) a piecemealI,JIt indicates,
Nano particle SEM image piecemeal average brightness matrix in this corresponding exampleFor shown in formula (2-1),
Matrix element aI,JEqual to nano particle SEM image piecemeal central point AI,JBrightness, wherein the I=1 of the present embodiment, 2,3,4;J=1,2,3,4,5,6.
The whole image average brightness of the nano particle SEM image of brightness irregularities is calculated using formula (3-1)
The whole image average brightness of the nano particle SEM image of brightness irregularities in this corresponding exampleIt is 94.6.
Figure 12 shows linear to viewing area brightness progress poor using 4 neighbour's differential techniques in the embodiment of the present invention 1 Value obtains the image of whole image brightness.
1 method can extract each brightness, concrete operations in nano particle SEM image through the embodiment of the present invention It is as follows:
C in nano particle SEM imageI,J,i,jThe brightness of point is cI,J,i,jPass through four point B of neighbourI,J、BI,J+1、BI+1,J+1 And BI+1,JCorresponding brightness be bI,J、bI,J+1、bI+1,J+1And bI+1,JIt finds out,
In formula (15-1), in the present embodiment, i=1,2 ..., 100;J=1,2 ..., 100;X and y respectively indicate pixel Point Ci,jApart from side BI,JBI,J+1Vertical range and apart from side BI,JBI+1,JVertical range, unit is pixel, and
0≤x≤100,0≤y≤100;
By matrix element gu,vConstitute the luminance matrix of the nano particle SEM image of brightness irregularities In matrix element gu,v With pixel Ci,jBrightness ci,jRelationship such as formula (17) shown in,
g(I-1)×m+i-m/2,(J-1)×n+j-n/2=cI,J,i,j(17),
In formula (17), in the present embodiment, i=1,2 ..., 100;J=1,2 ..., 100;I=1,2 ..., 5;J=1, 2 ..., 7;And meet 1≤[(I-1) × 100+i-50]≤400 and 1≤[(J-1) × 100+j-50]≤600 restrictive conditions, u =(I-1) × m+i-m/2, v=(J-1) × n+j-n/2;
With formula (18) display matrix member gi,jThe luminance matrix of brightness composition nano particle SEM image
So far it completes to extract the brightness that each is put in the nano particle SEM image of brightness irregularities;
Figure 13 shows the image in 1 nano particle SEM image whole region of the embodiment of the present invention after gamma correction, it will be seen that The effect of the 1 method correction nano particle SEM image provided through the embodiment of the present invention.
With the average brightness of the nano particle SEM image of brightness irregularitiesOn the basis of, calculate the correction system of each point NumberAnd the gamma correction q of each point of the nano particle SEM image of brightness irregularities is realized with formula (19)u,v,
In formula (18), Pu,vIndicate longitudinal u-th of pixel and lateral v in the nano particle SEM image of brightness irregularities The brightness of one point of a pixel, in this implementation, u=1,2 ..., 400;V=1,2 ..., 600;By formula (3) be calculated for The average brightness of the nano particle SEM image of brightness irregularities
As shown in formula (20), the gamma correction q of each point of the nano particle SEM image of brightness irregularitiesu,vIt constitutes The matrix of the nano particle SEM gamma correction image of brightness irregularities
The matrix of the nano particle SEM gamma correction image of brightness irregularitiesShow that nano particle SEM image is uneven The effect of the correction of brightness.
Figure 13 shows the effect that nano particle SEM image luminance uniformity correction is realized in embodiment of the present invention method 1, Show practicability of the invention.
Embodiment 1
The nano particle SEM image of brightness irregularities is divided into M × N number of piecemeal by the first step:
Nano particle SEM image is indicated with P, and the nano particle SEM image of wherein brightness irregularities is divided into longitudinal M= 4 equal portions and transverse direction N=6 equal portions, total 4 × 6 piecemeals do not overlap each other between piecemeal and piecemeal other than adjacent block boundary, In each piecemeal, longitudinally there is m=100 pixel, laterally there is n=100 pixel, the longitudinal direction of corresponding entire nano particle SEM image P 4 × 100 pixels and laterally 6 × 100 pixels, by the corresponding actual range of pixel, come calculate nano particle SEM image P and The actual size of each piecemeal, the quantity that pixel is covered in single segmented areas is >=10 nano particles, and brightness is not The vertical and horizontal of uniform nano particle SEM image include at least 3 segmented areas, and the laterally wide of each piecemeal is 100 Pixel, longitudinal a height of 100 pixel are met with guaranteeing to divide the stability of average brightness in nano particle SEM image segmented areas Average brightness is not influenced substantially by single nanoparticle in block region, ensure that the average brightness that segmented areas calculates is linearly outer The needs pushed away;
Second step calculates the average brightness and whole image average brightness of each segmented areas image:
To the block image that the nano particle SEM image of wherein brightness irregularities is divided by the above-mentioned first step, calculate every The average brightness of a segmented areas image is the brightness of piecemeal central point, and assigns matrix expression all piecemeal average brightness, Concrete operations are as follows:
The average brightness a of (I, J) a piecemeal of longitudinal label I and lateral label JI,JIt is calculated with formula (1),
In formula (1), Pu,vIndicate longitudinal u-th of pixel and lateral v in the nano particle SEM image of brightness irregularities The brightness of one point of a pixel, longitudinal label I=1,2 ..., M;Lateral label J=1,2 ... .., N;
In the present embodiment, m=100, n=100, M=4, N=6, shown in being specifically expressed as follows in formula (1),
Longitudinal label I=1,2,3,4;Lateral label J=1,2 ... .., 6;
With average brightness aI,JThe average brightness of all piecemeals is constituted into nano particle SEM image piecemeal according to the position of block Average brightness matrixAs shown in formula (2),
In formula (2), M is longitudinal equal portions of the nano particle SEM image piecemeal of brightness irregularities, and N is brightness irregularities Nano particle SEM image piecemeal lateral equal portions, the nano particle SEM image piecemeal average brightness matrix of brightness irregularities Matrix element aI,JThe central point of each piecemeal of the non-uniform nano particle SEM image of corresponding brightness is AI,J, wherein Zong Xiangbiao Number I=1,2 ..., M;Lateral label J=1,2 ..., N;Thus the calculating of the average brightness of each segmented areas image is completed;
In the present embodiment, the specific data of formula (2) are as follows,
The whole image average brightness of the nano particle SEM image of brightness irregularities is calculated using formula (3)This implementation In example, the specific data of formula (3) are as follows,
In formula (3), M=4 is longitudinal equal portions of the nano particle SEM image piecemeal of brightness irregularities in the present embodiment, N =6 be in the present embodiment be brightness irregularities nano particle SEM image piecemeal lateral equal portions, aI,JIt is longitudinal for average brightness Label I=1,2 ..., M;Lateral label J=1,2 ... .., N;
Thus the calculating of the average brightness and whole image average brightness of each segmented areas image is completed;
Third step obtains linear extrapolation by piecemeal average brightness matrix and expands luminance matrix:
To the nano particle SEM image piecemeal average brightness matrix for the brightness irregularities that above-mentioned second step obtainsCarry out line Property extrapolation expand, obtain linear extrapolation expand luminance matrixAs expand the matrix of M+2 row N+2 columnConcrete operations are such as Under:
Firstly, order matrixThe first row, last line, first row and last column matrix element be 0, intermediate matrix Member by with matrixComposition, matrixWith matrixBetween meet relationship shown in following formula (4),
In the present embodiment, the specific data of formula (4) are as follows,
For this purpose, expanding matrixFor shown in following formula (5),
Expand matrixFor shown in following formula (6),
In the present embodiment, byMatrix supplement 0 obtains matrixIt is as follows,
Secondly, expanding method according to linear extrapolation, above-mentioned expansion matrix is modifiedThe first row and last line matrix element Following formula (7) and formula (8),
b1,J=2 × b2,J-b3,J(7),
bM+2,J=2 × bM+1,J-bM,J(8),
In the present embodiment, M=4 in formula (8) has
b6,J=2 × b5,J-b4,J(8-1),
In formula (7), formula (8) and formula (8-1), lateral label J=2,3 ..., 7;
Equally, above-mentioned expansion matrix is modifiedFirst row and last column following formula of matrix element (9) and formula (10),
bI,1=2 × bI,2-bI,3(9),
bI,N+2=2 × bI,N+1-bI,N(10),
In the present embodiment, N=6 in formula (10) has
bI,8=2 × bI,7-bI,6(10-1),
In formula (9), formula (10) and formula (10-1), longitudinal label I=2,3,4,5;
For above-mentioned expansion matrixMatrix element b on four angles1,1、b1,N+2、bM+2,1And bM+2,N+2Respectively following formula (11), formula (12), formula (13) and formula (14),
In the present embodiment, formula (11)-(14) are respectively following data,
Linear extrapolation is obtained as a result, expands luminance matrixShown in following formula (15),
In formula (15), brightness b1,JCorresponding spatial point BI,JBrightness, spatial point BI,JCoordinate be laterally (J-1/2) × n + 1 pixel and longitudinal (I-1/2) × m+1 pixel, here, lateral label J=1,2 ..., N+2;Longitudinal label I=1,2 ..., M+ 2;
In the present embodiment, formula (15) is following data,
In the present embodiment, brightness bI,JCorresponding points BI,JBrightness, BI,JCoordinate is lateral (J-1/2) × 100+1 pixel, is indulged To (I-1/2) × 100+1 pixel, I=1 here, 2 ..., 6;J=1,2 ..., 8;
Figure 11 shows the image of each piecemeal average brightness of nano particle SEM image in embodiment 1.In the present embodiment Nano particle SEM image is by being laterally divided into 6 equal portions and being divided longitudinally into 4 equal portions, calculation shows that different piecemeal average brightness exist Difference illustrates the average brightness of nano particle SEM image in the present embodiment being divided into 24 piecemeals, shows that different piecemeals are deposited Difference, upper left corner piecemeal is brighter, and the lower right corner is darker;
Thus it completes to obtain linear extrapolation expansion luminance matrix by piecemeal average brightness matrix;
4th step extracts the brightness of each point in the nano particle SEM image of brightness irregularities:
(4.1) step determines each brightness in four surrounded regions of point:
Matrix is expanded by linear extrapolation obtained in above-mentioned third stepIn neighbouring four matrix element bI,J、bI,J+1、 bI+1,J+1And bI+1,J, corresponding rectangular BI,JBI,J+1BI+1,J+1BI+1,JFour point BI,J、BI,J+1、BI+1,J+1And BI+1,JIt is right Brightness is answered, the linear extrapolation is calculated with formula (16) and expands matrixIn neighbouring corresponding four points of four matrix elements BI,JBI,J+1BI+1,J+1BI+1,JSurround each point C in rectangular rangeI,J,i,jBrightness cI,J,i,j,
In formula (16), rectangular BI,JBI,J+1BI+1,J+1BI+1,JInterior longitudinal direction ith pixel, i=1,2 ..., m;Rectangular BI, JBI,J+1BI+1,J+1BI+1,JJ-th of pixel of interior transverse direction, j=1,2 ..., n;Longitudinal label I=1,2 ..., M+1;Lateral label J= 1,2 ..., N+1;X is pixel Ci,jApart from side BI,JBI,J+1Vertical range, y be pixel Ci,jApart from side BI,JBI+1,JHang down Straight distance, unit are pixel, and 0≤x≤m, 0≤y≤n;
In the present embodiment, formula (16) is following data,
Here i=1,2 ..., 100;J=1,2 ..., 100;I=1,2 ..., 5;J=1,2 ..., 7;0≤x≤100,0≤ y≤100;
(4.2) step extracts the brightness of each point in the nano particle SEM image of brightness irregularities:
By matrix element guvConstitute the luminance matrix of the nano particle SEM image of brightness irregularities In matrix element gu,v With pixel Ci,jBrightness ci,jRelationship such as formula (17) shown in,
g(I-1)×m+i-m/2,(J-1)×n+j-n/2=cI,J,i,j(17),
In formula (17), g(I-1)×m+i-m/2,(J-1)×n+j-n/2That is matrix element gu,v, i=1,2 ..., m;J=1,2 ..., n;I =1,2 ..., M+1;J=1,2 ..., N+1;It is constrained to 1≤[(I-1) × m+i-m/2]≤M × m and 1≤[(J-1) × n+j-n/ 2]≤N × n, u=(I-1) × m+i-m/2, v=(J-1) × n+j-n/2;
In the present embodiment, i=1,2 ..., 100;J=1,2 ..., 100;I=1,2 ..., 5;J=1,2 ..., 7;It is constrained to 1≤[(I-1) × 100+i-50]≤400 and 1≤[(J-1) × 100+j-50]≤600, u=(I-1) × 100+i-50, v= (J-1)×100+j-50;
By matrix element gu,vThe luminance matrix of the nano particle SEM image of the brightness irregularities of compositionFollowing formula (18) It is shown,
So far it completes to extract the brightness that each is put in the nano particle SEM image of brightness irregularities;
5th step calculates every bit brightness correction coefficients, realizes the correction of nano particle SEM image uneven brightness:
The whole image average brightness of the nano particle SEM image of brightness irregularities is calculated with formula (3)On the basis of, meter Calculate the correction coefficient of each pointAnd each of the nano particle SEM image of brightness irregularities is realized with formula (19) The gamma correction q of pointu,v,
In formula (19), Pu,vIndicate longitudinal ith pixel and lateral jth in the nano particle SEM image of brightness irregularities The brightness of one point of a pixel, u=1,2 ..., M × m;V=1,2 ..., N × n;For non-uniform nano particle SEM figure The whole image average brightness of picture, in the present embodiment, u=1,2 ..., 400;V=1,2 ..., 600;
As shown in formula (20), the gamma correction g of each point of the nano particle SEM image of brightness irregularitiesu,vIt constitutes The matrix of the nano particle SEM gamma correction image of brightness irregularities
The matrix of the nano particle SEM gamma correction image of brightness irregularitiesShow that nano particle SEM image is uneven The effect of the correction of brightness;
So far it completes to calculate every bit brightness correction coefficients, realizes the correction of nano particle SEM image uneven brightness.
Figure 13 shows the effect that nano particle SEM image luminance uniformity correction is realized in embodiment of the present invention method 1, Compared to the nano particle SEM image used in the embodiment of the present invention 1 shown by Figure 10, Figure 13 by the method for the present invention correction is aobvious The nano particle SEM image brightness uniformity shown is obviously improved, and shows practicability of the invention.
In above-described embodiment, the linear interpolation method is the known method of the art, related operation and calculating It is that those skilled in the art will appreciate that.

Claims (3)

1. the brightness of image bearing calibration extracted based on nano particle SEM image brightness, it is characterised in that: be a kind of based on nanometer Particle SEM image carries out whole brightness and extracts the corrected method of whole region uneven brightness to the image in turn, Specific step is as follows:
The nano particle SEM image of brightness irregularities is divided into M × N number of piecemeal by the first step:
Nano particle SEM image indicates with P, by the nano particle SEM image of wherein brightness irregularities be divided into longitudinal M equal portions and Lateral N equal portions, total M × N number of piecemeal do not overlap each other between piecemeal and piecemeal other than adjacent block boundary, each piecemeal In, longitudinally there is m pixel, laterally there is n-pixel, m and n are even number, longitudinal M × m picture of corresponding entire nano particle SEM image P Element and lateral N × n-pixel, by the corresponding actual range of pixel, to calculate nano particle SEM image P and each piecemeal Actual size, the quantity that pixel is covered in single segmented areas is >=10 nano particles, the nanometer of brightness irregularities The vertical and horizontal of particle SEM image include at least 3 segmented areas;
Second step calculates the average brightness and whole image average brightness of each segmented areas image:
To the block image that the nano particle SEM image of wherein brightness irregularities is divided by the above-mentioned first step, each point is calculated The average brightness of block area image is the brightness of piecemeal central point, and assigns matrix expression all piecemeal average brightness, specifically It operates as follows:
The average brightness a of (I, J) a piecemeal of longitudinal label I and lateral label JI,JIt is calculated with formula (1),
In formula (1), Pu,vIndicate longitudinal u-th of pixel and lateral v-th of picture in the nano particle SEM image of brightness irregularities The brightness of one point of element, longitudinal label I=1,2 ..., M;Lateral label J=1,2 ... .., N;
With average brightness aI,JThe average brightness of all piecemeals nano particle SEM image piecemeal is constituted according to the position of block to be averaged Luminance matrixAs shown in formula (2),
In formula (2), M is longitudinal equal portions of the nano particle SEM image piecemeal of brightness irregularities, and N is receiving for brightness irregularities The lateral equal portions of rice grain SEM image piecemeal, the nano particle SEM image piecemeal average brightness matrix of brightness irregularitiesSquare Array element aI,JThe central point of each piecemeal of the non-uniform nano particle SEM image of corresponding brightness is AI,J, wherein longitudinal label I =1,2 ..., M;Lateral label J=1,2 ..., N;Thus the calculating of the average brightness of each segmented areas image is completed;
The whole image average brightness of the nano particle SEM image of brightness irregularities is calculated using formula (3)
In formula (3), M is longitudinal equal portions of the nano particle SEM image piecemeal of brightness irregularities, and N is receiving for brightness irregularities The lateral equal portions of rice grain SEM image piecemeal, aI,JFor average brightness, longitudinal label I=1,2 ..., M;Lateral label J=1, 2 ... .., N;
Thus the calculating of the average brightness and whole image average brightness of each segmented areas image is completed;
Third step obtains linear extrapolation by piecemeal average brightness matrix and expands luminance matrix:
To the nano particle SEM image piecemeal average brightness matrix for the brightness irregularities that above-mentioned second step obtainsIt carries out linear outer Expansion is pushed away, linear extrapolation is obtained and expands luminance matrixAs expand the matrix of M+2 row N+2 columnConcrete operations are as follows:
Firstly, order matrixThe first row, last line, first row and last column matrix element be 0, intermediate matrix element by With matrixComposition, matrixWith matrixBetween meet relationship shown in following formula (4),
For this purpose, expanding matrixFor shown in following formula (5),
Expand matrixFor shown in following formula (6),
Secondly, expanding method according to linear extrapolation, above-mentioned expansion matrix is modifiedThe first row and last line matrix element it is public as follows Formula (7) and formula (8),
b1,J=2 × b2,J-b3,J(7),
bM+2,J=2 × bM+1,J-bM,J(8),
In formula (7) and formula (8), lateral label J=2,3 ..., N+1;
Equally, above-mentioned expansion matrix is modifiedFirst row and last column following formula of matrix element (9) and formula (10), bI,1 =2 × bI,2-bI,3(9),
bI,N+2=2 × bI,N+1-bI,N(10),
In formula (9) and formula (10), longitudinal label I=2,3 ..., M+1;
For above-mentioned expansion matrixMatrix element b on four angles1,1、b1,N+2、bM+2,1And bM+2,N+2Respectively following formula (11), Formula (12), formula (13) and formula (14),
Linear extrapolation is obtained as a result, expands luminance matrixShown in following formula (15),
In formula (15), brightness b1,JCorresponding spatial point BI,JBrightness, spatial point BI,JCoordinate be lateral (J-1/2) × n+1 picture Element and longitudinal (I-1/2) × m+1 pixel, here, lateral label J=1,2 ..., N+2;Longitudinal label I=1,2 ..., M+2;
Thus it completes to obtain linear extrapolation expansion luminance matrix by piecemeal average brightness matrix;
4th step extracts the brightness of each point in the nano particle SEM image of brightness irregularities:
(4.1) step determines each brightness in four surrounded regions of point:
Matrix is expanded by linear extrapolation obtained in above-mentioned third stepIn neighbouring four matrix element bI,J、bI,J+1、bI+1,J+1 And bI+1,J, corresponding rectangular BI,JBI,J+1BI+1,J+1BI+1,JFour point BI,J、BI,J+1、BI+1,J+1And BI+1,JCorresponding brightness, The linear extrapolation, which is calculated, with formula (16) expands matrixIn the neighbouring corresponding four points B of four matrix elementsI,JBI,J+ 1BI+1,J+1BI+1,JSurround each point C in rectangular rangeI,J,i,jBrightness cI,J,i,j,
In formula (16), rectangular BI,JBI,J+1BI+1,J+1BI+1,JInterior longitudinal direction ith pixel, i=1,2 ..., m;Rectangular BI,JBI,J+ 1BI+1,J+1BI+1,JJ-th of pixel of interior transverse direction, j=1,2 ..., n;Longitudinal label I=1,2 ..., M+1;Lateral label J=1, 2 ..., N+1;X is pixel Ci,jApart from side BI,JBI,J+1Vertical range, y be pixel Ci,jApart from side BI,JBI+1,JIt is vertical Distance, unit are pixel, and 0≤x≤m, 0≤y≤n;
(4.2) step extracts the brightness of each point in the nano particle SEM image of brightness irregularities:
By matrix element gu,vConstitute the luminance matrix of the nano particle SEM image of brightness irregularitiesIn matrix element gu,vWith Pixel Ci,jBrightness ci,jRelationship such as formula (17) shown in,
g(I-1)×m+i-m/2,(J-1)×n+j-n/2=cI,J,i,j(17),
In formula (17), g(I-1)×m+i-m/2,(J-1)×n+j-n/2That is matrix element gu,v, i=1,2 ..., m;J=1,2 ..., n;I=1, 2 ..., M+1;J=1,2 ..., N+1;Be constrained to 1≤[(I-1) × m+i-m/2]≤M × m and 1≤[(J-1) × n+j-n/2]≤ N × n, u=(I-1) × m+i-m/2, v=(J-1) × n+j-n/2;
By matrix element gu,vThe luminance matrix of the nano particle SEM image of the brightness irregularities of compositionShown in following formula (18),
In formula (18), longitudinal label u=1,2 ..., M × m, lateral label v=1,2 ..., N × n;
So far it completes to extract the brightness that each is put in the nano particle SEM image of brightness irregularities;
5th step calculates every bit brightness correction coefficients, realizes the correction of nano particle SEM image uneven brightness:
The whole image average brightness of the nano particle SEM image of brightness irregularities is calculated with formula (3)On the basis of, it calculates every The correction coefficient of one pointAnd each point of the nano particle SEM image of brightness irregularities is realized with formula (19) Gamma correction qu,v,
In formula (19), Pu,vIndicate longitudinal u-th of pixel and lateral v-th of picture in the nano particle SEM image of brightness irregularities The brightness of one point of element, u=1,2 ..., M × m;V=1,2 ..., N × n;For non-uniform nano particle SEM image Whole image average brightness,
As shown in formula (20), the gamma correction g of each point of the nano particle SEM image of brightness irregularitiesu,vConstitute brightness The matrix of non-uniform nano particle SEM gamma correction image
The matrix of the nano particle SEM gamma correction image of brightness irregularitiesShow nano particle SEM image uneven brightness Correction effect;
So far it completes to calculate every bit brightness correction coefficients, realizes the correction of nano particle SEM image uneven brightness.
2. the brightness of image bearing calibration extracted according to claim 1 based on nano particle SEM image brightness, feature are existed In: it is described that the nano particle SEM image of wherein brightness irregularities is divided into longitudinal M equal portions and transverse direction N equal portions, wherein M=4, N=6.
3. the brightness of image bearing calibration extracted according to claim 1 based on nano particle SEM image brightness, feature are existed In: in each described piecemeal, longitudinally there is m pixel, laterally there is n-pixel, wherein n=100 pixel, m=100 pixel.
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