CN109468362A - A kind of accurate identification method of ratoon sugarcane hoja blanca resistance - Google Patents
A kind of accurate identification method of ratoon sugarcane hoja blanca resistance Download PDFInfo
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- CN109468362A CN109468362A CN201910042437.4A CN201910042437A CN109468362A CN 109468362 A CN109468362 A CN 109468362A CN 201910042437 A CN201910042437 A CN 201910042437A CN 109468362 A CN109468362 A CN 109468362A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- G—PHYSICS
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- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
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Abstract
The present invention discloses a kind of accurate identification method of ratoon sugarcane hoja blanca resistance, comprising: prepares for the processing of examination Sugarcane smut with plantation, inoculation liquid, cuts the inoculation of stem inocalation method, Disease investigation, disease resistance evaluation.The method of the present invention creates the accurate identification method of the ratoon sugarcane hoja blanca resistance of a set of simple and effective for the first time, provides technical support for anti-hoja blanca breeding；Its inoculation method uses and cuts stem inocalation method, similar to natural propagation, has morbidity habitat conditions, morbidity uniformly, as a result objective, true, reliable；Inoculation method is fast and convenient, and strong operability, inoculation work efficiency are high.Sugarcane white leaf is fallen ill after inoculation, and significant, high sensitivity, repeatability are good.
Description
Technical field
The invention belongs to crop resistance identification technology fields, and in particular to a kind of ratoon sugarcane hoja blanca resistance it is accurate
Identification method.
Background technique
Sugarcane white leaf (Sugarcane white leaf, SCWL) is first by phytoplasma on China sugarcane
The important new expression of quarantine risk caused by (Sugarcane white leaf phytoplasma), diffusion sprawling are very fast
Speed seriously threatens China's sugarcane production and development.Sugarcane white leaf was found in Northern Thailand Nan Bangfu for the first time in 1954, existing
Betide the countries in Southeast Asia such as India, Pakistan, Sri Lanka, Japan, Laos, Burma, Vietnam, Philippine extensively.
SCWL is a kind of extremely hazardous phytoplasma disease, can cause crushing disaster to sugarcane.5%- is reached in Thailand's SCWL disease incidence
35%, it brings lost more than 20,000,000 dollars every year.In New Guinea, which causes cultivar glug to receive loss to be up to
100%, very big economic loss is caused to locality.2012 Nian Liwen phoenix etc. detect discovery SCWL phytoplasma in Baoshan, Yunnan for the first time,
Occurring area has expanded to 20,000 mu or more at present；2013-2017, successively the county Lincang Geng Ma, Zhenkang County, the county Shuan Jiang,
The sugarcane districts such as the county Cang Yuan, Linxiang District and Pu'er county Xi Meng detect SCWL phytoplasma, and generation area area is more than 300,000 mu at present, and
In extension sprawling increasingly and aggravate situation.After sugarcane infects SCWL phytoplasma, plant height, stem diameter are obviously dropped at stem rate and single stem weight
Low or reduction, causes the significantly underproduction to subtract sugar.Ratoon sugarcane accounts for 2/3rds or more in China's sugarcane district cultivated area, according to investigations,
Cane yield 6-7 tons per acre of severely afflicated area new plant cane, 1 year stubble cane effectively causes destruction without receipts because of SCWL harm.SCWL is in Yunnan
Sugarcane district extension sprawling is very rapid, brings severe disaster threat to Yunnan sucrose industry development, such as cannot get effective prevention and control, can
Also potential threat can be brought to sugarcane districts such as Guangxi, Guangdong.
Breeding and the anti-hoja blanca sugar cane breed of popularization are the most economical effective methods of prevention and control Sugarcane white leaf evil, and most
The green ecological Control Technology of potentiality.Sugarcane white leaf is the new expression that Quick Extended gets up in recent years, not yet establishes perennial root so far
The anti-hoja blanca identification method of sugarcane.How the hoja blanca disease resistance of accurate efficient evaluations sugar cane breed is current cane breeding man
The technical bottleneck that the anti-hoja blanca kind of breeding faces.It extends sprawling increasingly in view of China's Sugarcane white leaf and aggravates situation,
Therefore it is badly in need of the accurate identification method that a set of easy, efficient ratoon sugarcane hoja blanca resistance is established in exploitation, pushes anti-hoja blanca
The anti-hoja blanca breeding research blank of domestic ratoon sugarcane is filled up in the development of breeding work.
Summary of the invention
Sugarcane production is seriously threatened for quarantine new expression Sugarcane white leaf and lacks the production of method of resistance identification again
Industry technical problem, the present invention provide a kind of accurate identification method of easy, efficient ratoon sugarcane hoja blanca resistance.
A kind of accurate identification method of ratoon sugarcane hoja blanca resistance provided by the invention, comprising the following steps:
(1) for the processing of examination Sugarcane smut and plantation: to be susceptible check variety, with anti-to the susceptible sugar cane breed of hoja blanca
The sugar cane breed of hoja blanca is disease-resistant check variety；By susceptible check variety, disease-resistant check variety and sugarcane material to be identified
Material is cut into the sugarcane stem section with 1-2 bud respectively, after impregnating 48h in flowing running water respectively, respectively with 50 DEG C ± 0.5 DEG C
Hot water treatment 2h, then pesticide-germicide mixed liquid dipping 10min is used respectively, it then will treated susceptible check variety cane stalk
Section, disease-resistant check variety sugarcane stem section and Sugarcane smut sugarcane stem section to be identified are planted in the plastic barrel for filling matrix respectively
It is interior, it is placed in 20 DEG C~30 DEG C insect prevention greenhouses and cultivates, every part of check variety and 3~4 repetitions of Sugarcane smut to be identified；
(2) inoculation liquid is prepared: being cut the sugarcane sugarcane stem for having infected Sugarcane white leaf phytoplasma, has been infected sugarcane by squeezing
The sugarcane sugarcane stem of hoja blanca phytoplasma obtains carrying Sugarcane white leaf phytoplasma sugarcane juice, is incorporated as carrying Sugarcane white leaf phytoplasma
The 10 times of amount sterile water dilutions of sugarcane juice volume mix, and are filtered with double gauze, and filtrate is the inoculation of Sugarcane white leaf phytoplasma cause of disease
Liquid, it is ready-to-use；
(3) inoculation of stem inocalation method is cut: when Sugarcane smut to be identified 6 months or more strain ages when be planted in step (1),
The cloudy day dusk with sterilize sharp cutter or the secateurs that sterilizes by the Sugarcane smut to be identified being planted in plastic barrel, susceptible right
It cuts, is used when cutting the sugarcane plant in every barrel after sterilizing not according to kind, the plant of disease-resistant check variety on the substrate surface
Then the sharp cutter or secateurs used connects susceptible check variety, disease-resistant check variety and Sugarcane smut to be identified
Kind, vaccination ways are that Sugarcane white leaf phytoplasma cause of disease inoculation liquid described in 100 μ L steps (2) is instilled one plant of sugarcane with liquid-transfering gun
On the notch of strain root, every part of susceptible check variety, disease-resistant check variety and Sugarcane smut to be identified are inoculated with 30 plants, are inoculated with subsequent
Continuous be placed in 20 DEG C~30 DEG C insect prevention greenhouses cultivates observation, and first with black plastic film by the susceptible control after inoculation after being inoculated with
Kind, disease-resistant check variety and Sugarcane smut shading to be identified for 24 hours, then open black plastic film；
(4) Disease investigation: after inoculation 20d, investigation records is respectively for trying Sugarcane smut diseased plant percentage, until susceptible reference substance
Until the diseased plant percentage of kind is stablized；
(5) it disease resistance evaluation: is commented according to Sugarcane smut diseased plant rate to be identified by 1-5 grades of Sugarcane white leaf assessment resistances
Price card standard carries out Sugarcane white leaf disease resistance evaluation, and the 1-5 grades of Sugarcane white leaf assessment resistance evaluation criterion successively indicates pair
Sugarcane white leaf phytoplasma highly resistance, disease-resistant, middle resistant, susceptible and high sense, diseased plant rate range mutually should be 0%-3.0%,
3.1%-10.0%, 10.1%-20.0%, 20.1%-40.0% and 40.1%-100%.
It with pesticide-germicide mixed liquid dipping 10min is with 70% Diacloden water dispersant and 50% described in step (1)
800 times of liquid of carbendazol wettable powder impregnate 10min.
Matrix described in step (1) is mixed by disinfection soil and disinfection well-rotted farmyard manure by 3:1 volume ratio.
Susceptible check variety described in step (1) is Guangdong sugar 86-368, and disease-resistant check variety is Guangdong sugar 83-88.
The advantages of the present invention:
1, the accurate identification method for creating a set of easy, efficient ratoon sugarcane hoja blanca resistance for the first time, has filled up state
The interior anti-hoja blanca breeding research blank of ratoon sugarcane provides key technology support for the anti-hoja blanca breeding of China's ratoon sugarcane.
It can be realized using the method for the present invention to the fast of sugarcane quality germplasm, common hybrid parent and new varieties (being) anti-hoja blanca
Speed, precisely evaluation, will improve conscientiously the accuracy and Breeding Efficiency of anti-hoja blanca breeding, for anti-hoja blanca Screening of Germplasm
It selects to be of great significance and realistic function with kind with production.
2, using the inoculation of stem inocalation method is cut, the wound of manufacture is uniform, and inoculation cause of disease amount is consistent, has and meet the white leaf of sugarcane
Disease infects the habitat conditions of morbidity, falls ill uniformly after inoculation, can intuitively show sugar cane breed to the resistance water of Sugarcane white leaf
Flat, qualification result is objective, true, reliable.
It 3, is the main path that Sugarcane white leaf is propagated due to cutting tool cross-infection with poison receipts, and growth period cuts stem inoculation
Method and such circulation way are much like, therefore qualification result is closer to the naturally susceptible result in field.
4, inoculum of the invention does not have to be separately cultured, and directly acquires and prepares from field.Inoculation method is fast and convenient, can grasp
The property made is strong, inoculation work efficiency is high.Sugarcane white leaf is fallen ill after inoculation, and significant, high sensitivity, repeatability are good, therefore can be to high-volume
Material is identified and is screened, and provides more widely disease-resistant variety and anti-source materials for breeding and production departments.
Specific embodiment
Following embodiment is not limited to protection scope of the present invention for the invention will be further described.Each reality
Applying in example without specified otherwise is conventional method.
(1) for examination sugar cane breed material processing and plantation
Choose Guangdong sugar No. 60, ROC25, be full of educate 91-59, Guangdong sugar 93-159, ROC22, cloud sugarcane 86-161, cloud sugarcane 03-194,
Totally 10 different sugar cane breeds make sugar cane breed material to be identified by ROC10, Liucheng 05-136, cloud sugarcane 05-51, with Guangdong sugar 86-
368 make susceptible check variety, Guangdong sugar 83-88 makees disease-resistant check variety.By susceptible check variety, disease-resistant check variety and wait reflect
Fixed sugar cane breed material is cut into double leaf stem sections of 2 buds of band respectively, after impregnating 48h in room temperature flowing running water respectively,
Respectively with 50 DEG C of ± 0.5 DEG C of hot water treatment 2h, then pesticide-germicide mixed liquid dipping 10min is used respectively, the pesticide-germicide mixing
Liquid is the i.e. available 70% Diacloden water dispersant of 800 times of liquid of 70% Diacloden water dispersant and 50% carbendazol wettable powder
It is made into after 18.75g and 50% carbendazol wettable powder 18.75g mixing with 15kg sterile water.
Double bud stems of treated sugar cane breed material to be identified, susceptible check variety and disease-resistant check variety
Section, is planted in respectivelyIn different plastic barrels, the base of the built-in promising plastic barrel volume 2/3 of plastic barrel
Matter, the matrix is by disinfection soil and disinfection well-rotted farmyard manure by disinfection soil: it is mixed for 3:1 to sterilize the volume ratio of well-rotted farmyard manure
It closes.The disinfection soil and disinfection well-rotted farmyard manure are to be disappeared respectively with boiling a heatable brick bed to soil and well-rotted farmyard manure boiling 1h
Poison.
Every part of sugar cane breed material to be identified, susceptible check variety and disease-resistant check variety material plant 4 barrels i.e. 4 weights
Multiple, every barrel of 8 buds, every part of material totally 32 bud is placed in 20 DEG C of Sugarcane Inst., Yunnan Prov. Agriculture Academy's (Yunnan Province's Kaiyuan City)
Observation is cultivated in~30 DEG C of Resistant identification insect prevention greenhouses.
(2) inoculation liquid is prepared:
There is the high sense kind of typical hoja blanca symptom from the selection of Yunnan Province Lincang Geng Maxian Sugarcane white leaf diseased region
The sugarcane stem that Guangdong sugar 86-368 sugarcane strain detects screening phytoplasma containing Sugarcane white leaf through nest-type PRC makees inoculation cause of disease.Pass through before inoculation
The sugarcane stem that inoculation cause of disease is made in squeezing, which obtains, carries Sugarcane white leaf phytoplasma sugarcane juice, is incorporated as carrying Sugarcane white leaf phytoplasma sugarcane
The sterile water of 10 times of juice volume amounts, which dilutes, to be mixed, and is filtered with double gauze, and filtrate is the inoculation of Sugarcane white leaf phytoplasma cause of disease
Liquid, it is ready-to-use.
Typical case's hoja blanca symptom is that diseased plant blade shows as food value of leaf softness, albefaction, and tiller increased significantly.The whole leaf having
Albefaction, some are in striated albefaction, and some is in mottling albefaction.
The nest-type PRC is detected as conventional method, and referring to Li Wenfeng, WANG XIAOYAN, Huang answers the important disease molecule of the sugarcane such as elder brother
Detection technique plant protection, 2016,42 (5): 125-130 is specifically included that
First time PCR amplification:
Using the universal primer of amplification phytoplasma 16S rDNA to P1/P7, in the nucleotide sequence of primer P1 such as sequence table
Shown in SEQ ID NO:1, the nucleotide sequence of primer P7 is as shown in SEQ ID NO:2 in sequence table.
0.2g Sugarcane Leaves are taken, liquid nitrogen grinding powdering is added, leaf is extracted using DNA Kit plant DNA extraction kit
Piece total DNA, specific steps are operated according to kit specification, albumen/nucleic acids instrument identification of dna quality are used after extraction, with P1/
P7 is that primer carries out the 1st PCR amplification, and PCR reaction system is 25 μ L: 1 μ L, 10 × PCR buffer2.5 μ of total genomic dna
L, P1 (20 μm of ol/L) 1 μ L, P7 (20 μm of ol/L) 1 μ L, MgCl2(25mmol/L) 2.0 μ L, dNTPs (10mmol/L) 2.0 μ L,
Taq enzyme (5U/ μ L) 0.2 μ L, ddH2O 15.3μL.1st PCR amplification program are as follows: 94 DEG C of initial denaturation 3min；94 DEG C of denaturation 30s,
55 DEG C of annealing 30s, 72 DEG C of extension 1min, 35 recycle；Last 72 DEG C of extensions 10min.
Second of PCR amplification:
Using universal primer to R16F2n/R16R2, SEQ ID NO in the nucleotide sequence of primer R16F2n such as sequence table:
Shown in 3, the nucleotide sequence of primer R16R2 is as shown in SEQ ID NO:4 in sequence table.
2nd 25 μ L of PCR reaction system: taking the 1st pcr amplification product of 1 μ L (30 times of dilution) to be used as template DNA,
R16F2n (20 μm of ol/L) 1 μ L, R16R2 (20 μm of ol/L) 1 μ L, other reagents and its dosage are the same as the 1st PCR amplification.2nd time
PCR amplification program are as follows: 94 DEG C of initial denaturation 3min；94 DEG C of denaturation 30s, 57 DEG C of annealing 30s, 72 DEG C of extension 1min, 35 recycle；Most
72 DEG C of extension 10min afterwards.
It takes the 2nd pcr amplification product of 10 μ L in electrophoresis on 1.0% Ago-Gel, is seen with BIO-RAD gel imaging system
It examines, differentiate as a result, amplifying 1240bp band is that the SCWL positive contains Sugarcane white leaf phytoplasma, do not amplify 1240bp item
Band is SCWL feminine gender without Sugarcane white leaf phytoplasma.
(3) inoculation of stem inocalation method is cut: when 6 months strain ages of sugar cane breed material to be identified when be planted in step (1),
The cloudy day dusk with sterilize sharp cutter or sterilizing secateurs by the sugar cane breed material to be identified being planted in plastic barrel, feel
Sick check variety, the plant of disease-resistant check variety on the substrate surface cut, with sterilizing when cutting the sugarcane plant in every barrel
Afterwards not used sharp cutter or sterilizing after not used secateurs, the sharp cutter or secateurs with 75% alcohol sterilizing, so
Susceptible check variety, disease-resistant check variety and sugar cane breed material to be identified are inoculated with afterwards, vaccination ways are to use liquid relief
Rifle instills Sugarcane white leaf phytoplasma cause of disease inoculation liquid described in 100 μ L on one plant of sugarcane strain root notch, every part of susceptible reference substance
Kind, disease-resistant check variety and sugar cane breed material to be identified are inoculated with 30 plants, continue after inoculation disease-resistant at above-mentioned 20 DEG C~30 DEG C
Worm, which is identified, cultivates observation in insect prevention greenhouse, and first with black plastic film by the susceptible check variety, disease-resistant after inoculation after being inoculated with
Check variety and sugar cane breed material shading to be identified for 24 hours, then open black plastic film；
(4) Disease investigation: after inoculation 20d, investigation records is respectively for trying sugar cane breed material diseased plant percentage, until susceptible right
According to kind diseased plant percentage stablize until；Record project includes: the inoculation date, plant number, the Disease symptoms beginning is current, adds up hair
Diseased plant number.
(5) disease resistance evaluation: according to sugar cane breed material diseased plant rate to be identified by 1-5 grades of Sugarcane white leaf resistances point
Grade evaluation criterion carries out Sugarcane white leaf disease resistance evaluation, and see Table 1 for details for 1-5 grades of Sugarcane white leaf assessment resistance evaluation criterions.
1 1-5 grades of Sugarcane white leaf assessment resistance evaluation criterion of table
As shown in table 2, it is evaluated for examination sugar cane breed material diseased plant rate by 1-5 grades of Sugarcane white leaf assessment resistances according to each
Standard carries out Sugarcane white leaf disease resistance evaluation, in which: Guangdong sugar 60, ROC25, is full of and educates 91-59 and show as 5 grades high sense, Guangdong sugar
93-159, ROC22 show as 4 grades it is susceptible, cloud sugarcane 86-161 shows as resisting in 3 grades, cloud sugarcane 03-194, ROC10 show as 2 grades it is anti-
Disease, Liucheng 05-136, cloud sugarcane 05-51 show as 1 grade of highly resistance.It is respectively natural for examination sugar cane breed material Resistance Identification result and field
Incidence matches.
Resistance Identification result of the different sugar cane breeds of table 2 to Sugarcane white leaf
Sequence table
<110>Sugarcane Inst., Yunnan Prov. Agriculture Academy
<120>a kind of accurate identification method of ratoon sugarcane hoja blanca resistance
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 25
<212> DNA
<213>artificial sequence (Artificial Sequence)
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aagagtttga tcctggctca ggatt 25
<210> 2
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<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
cgtccttcat cggctctt 18
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<212> DNA
<213>artificial sequence (Artificial Sequence)
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gaaacgactg ctaagactgg 20
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<211> 25
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 4
tgacgggcgg tgtgtacaaa ccccg 25
Claims (4)
1. a kind of accurate identification method of ratoon sugarcane hoja blanca resistance, which comprises the following steps:
(1) for the processing of examination Sugarcane smut and plantation: to be susceptible check variety, with anti-Bai Ye to the susceptible sugar cane breed of hoja blanca
The sugar cane breed of disease is disease-resistant check variety；By susceptible check variety, disease-resistant check variety and Sugarcane smut to be identified point
It is not cut into the sugarcane stem section with 1-2 bud, after impregnating 48h in flowing running water respectively, respectively with 50 DEG C of ± 0.5 DEG C of hot water
2h is handled, then uses pesticide-germicide mixed liquid dipping 10min respectively, it then will treated susceptible check variety sugarcane stem section, anti-
Sick check variety sugarcane stem section and Sugarcane smut sugarcane stem section to be identified are planted in respectively in the plastic barrel for filling matrix, are set
It is cultivated in 20 DEG C~30 DEG C insect prevention greenhouses, every part of check variety and 3~4 repetitions of Sugarcane smut to be identified；
(2) inoculation liquid is prepared: cutting the sugarcane sugarcane stem for having infected Sugarcane white leaf phytoplasma, it is white to obtain carrying sugarcane by squeezing
Leaf disease phytoplasma sugarcane juice is incorporated as carrying the 10 times of amount sterile water dilution mixings of Sugarcane white leaf phytoplasma sugarcane juice volume, with bilayer
Filtered through gauze, filtrate is Sugarcane white leaf phytoplasma cause of disease inoculation liquid, ready-to-use；
(3) inoculation of stem inocalation method is cut: when Sugarcane smut to be identified 6 months or more strain ages when be planted in step (1), in yin
Sugarcane smut to be identified, the susceptible reference substance that it dusk will be planted in plastic barrel with sterilize sharp cutter or sterilizing secateurs
Kind, the plant of disease-resistant check variety on the substrate surface cut, with unused after sterilizing when cutting the sugarcane plant in every barrel
Sharp cutter or secateurs, then susceptible check variety, disease-resistant check variety and Sugarcane smut to be identified are inoculated with, connect
Kind mode is that Sugarcane white leaf phytoplasma cause of disease inoculation liquid described in 100 μ L steps (2) is instilled one plant of sugarcane strain root with liquid-transfering gun
On portion's notch, every part of susceptible check variety, disease-resistant check variety and Sugarcane smut to be identified are inoculated with 30 plants, continue to set after inoculation
Observation is cultivated in 20 DEG C~30 DEG C insect prevention greenhouses, and first with black plastic film by the susceptible reference substance after inoculation after being inoculated with
Kind, disease-resistant check variety and Sugarcane smut shading to be identified for 24 hours, then open black plastic film；
(4) Disease investigation: after inoculation 20d, investigation records is respectively for trying Sugarcane smut diseased plant percentage, up to susceptible check variety
Until diseased plant percentage is stablized；
(5) disease resistance evaluation: according to Sugarcane smut diseased plant rate to be identified by 1-5 grades of Sugarcane white leaf assessment resistance evaluation marks
Standard carries out Sugarcane white leaf disease resistance evaluation, and the 1-5 grades of Sugarcane white leaf assessment resistance evaluation criterion is successively indicated to sugarcane
Hoja blanca phytoplasma highly resistance, disease-resistant, middle resistant, susceptible and high sense, diseased plant rate range mutually should be 0%-3.0%, 3.1%-
10.0%, 10.1%-20.0%, 20.1%-40.0% and 40.1%-100%.
2. the accurate identification method of ratoon sugarcane hoja blanca resistance according to claim 1, it is characterised in that: step (1)
Described in be with 70% Diacloden water dispersant and 50% carbendazol wettable powder with pesticide-germicide mixed liquid dipping 10min
800 times of liquid impregnate 10min.
3. the accurate identification method of ratoon sugarcane hoja blanca resistance according to claim 1, it is characterised in that: step (1)
Described in matrix mixed by disinfection soil and disinfection well-rotted farmyard manure by 3:1 volume ratio.
4. the accurate identification method of ratoon sugarcane hoja blanca resistance according to claim 1, it is characterised in that: step (1)
Described in susceptible check variety be Guangdong sugar 86-368, disease-resistant check variety be Guangdong sugar 83-88.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114793812A (en) * | 2022-04-13 | 2022-07-29 | 华南农业大学 | Method for identifying sugarcane white streak disease resistance |
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| CN108486216A (en) * | 2018-03-08 | 2018-09-04 | 福建农林大学 | Resistance Identification method in sugar cane breed informal voucher ward |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108486216A (en) * | 2018-03-08 | 2018-09-04 | 福建农林大学 | Resistance Identification method in sugar cane breed informal voucher ward |
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| KAEWMANEE C，HANBOONSONG Y.: "Evaluation of the efficiency of various treatments used for sugarcane white", 《BULLETIN OF INSECTOLOGY》 * |
| 魏春燕等: "甘蔗白叶病研究概况", 《南方农业学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114793812A (en) * | 2022-04-13 | 2022-07-29 | 华南农业大学 | Method for identifying sugarcane white streak disease resistance |
| CN114793812B (en) * | 2022-04-13 | 2023-06-06 | 华南农业大学 | Method for identifying sugarcane white streak resistance |
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