CN109465861A - A kind of gel rubber knife adjusting glue thickness - Google Patents
A kind of gel rubber knife adjusting glue thickness Download PDFInfo
- Publication number
- CN109465861A CN109465861A CN201811558622.0A CN201811558622A CN109465861A CN 109465861 A CN109465861 A CN 109465861A CN 201811558622 A CN201811558622 A CN 201811558622A CN 109465861 A CN109465861 A CN 109465861A
- Authority
- CN
- China
- Prior art keywords
- handle
- rubber knife
- blade
- glue thickness
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003292 glue Substances 0.000 title claims abstract description 26
- 239000000463 material Substances 0.000 claims abstract description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 2
- 229920000297 Rayon Polymers 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000004064 recycling Methods 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 238000001742 protein purification Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26B—HAND-HELD CUTTING TOOLS NOT OTHERWISE PROVIDED FOR
- B26B27/00—Hand cutting tools not provided for in the preceding groups, e.g. finger rings for cutting string, devices for cutting by means of wires
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention patent discloses a kind of adjustable gel rubber knife for cutting glue thickness, is made of the screw of cutter head, adjusting thickness that material is 316 stainless steels and knife handle.The rubber knife total length is 14 centimetres, and handle is 2 centimetres wide, V-type bifurcation structure, cutter head is made of the U-shaped blade for being fixed on handle one side with the I shape blade for being fixed on handle another side, U-shaped blade two sides blade lengths are 1.5 centimetres, and middle single-slice length is 0.72 centimetre, 1.2 centimetres high；I type blade lengths are 0.7 centimetre, 1.2 centimetres high., can be at 0.05 centimetre by the screw knob in rotating handle --- the rubber knife is adjusted in 1 cm range cuts glue thickness.Cutting gel purpose band can be facilitated using gel rubber knife described in the invention, be convenient for subsequent colloidal sol and DNA recovery experiment.
Description
Technical field
The present invention relates to experimental instrument fields, and in particular to gel recycles tool in molecular biology experiment, especially solidifying
Glue rubber knife tool.
Background technique
Gel electrophoresis technology is the important technical for carrying out DNA and protein purification, and it is raw to be widely used in each molecule
Object experiment.Under normal conditions, DNA to be purified or protein molecule be after Ago-Gel or acrylamide gel electrophoresis,
After electrophoretic band identical with target DNA fragments or protein molecule size carries out gel extraction, through either physically or chemically right
Colloidal sol recycles after DNA or protein purification after being dissolved again, obtain purifying DNA or the protein of free from admixture for use in
Subsequent experimental analysis.
Although having the gel collection apparatus of automation at present, since it is expensive, and the target gel block recycled is big
Small to be difficult to adjust, domestic and international most of laboratories are usually still using craft gel extraction DNA or the side of protein target segment
Method recycles the target stripe on gel.It cuts glue tool and usually selects scalpel or plastic knife.But when cutting glue using these cutters,
On the one hand cumbersome, and the blob of viscose thickness cut is different, is not easy to the blob of viscose colloidal sol and DNA purification process of subsequent recovery, especially
It is to normally result in corresponding two centrifuge tubes because blob of viscose is not of uniform size when needing to carry out centrifugally operated and be difficult to balance.Therefore,
It is badly in need of a kind of easy to operate and can satisfy on the one hand to be set according to width of the purpose band on gel and cut glue thickness,
On the other hand it cuts under glue depth information having determined, it is ensured that same batch of sample can access cutting for the recycling blob of viscose of same size
Glue tool, so as to subsequent colloidal sol and DNA or protein purification experimental implementation.
Summary of the invention
In order to facilitate gel recycling, recycling blob of viscose of the same size is obtained for subsequent experimental, and the invention discloses one kind
The gel rubber knife of glue thickness can be adjusted.The gel rubber knife material be 316 stainless steels, by handle, adjust thickness screw and
Cutter head is constituted, and 14 centimetres of total length (Fig. 1).
Handle is 2 centimetres wide, is V-type bifurcation structure, and shape is similar with surgical forceps, and the installation of handle side end is U-shaped
Blade；I type blade (Fig. 2) is installed other side end.The handle side of the fixed U-shaped blade in centre contains the hand for wrapping up another side handle
Lever (Fig. 3).
Cutter head is made of U-shaped blade and I type blade, and U-shaped blade two sides blade lengths are 1.5 centimetres, middle single-slice length
It is 0.72 centimetre, it is 1.2 centimetres high；I type blade lengths are 0.7 centimetre, high 1.2 centimetres (Fig. 4).
Glue thickness adjustment knob is cut by the screw that is fixed on handle and the knob structure for being adjusted by rotation handle bifurcated size
At, instruction is wherein indicated on screw and cuts the number of glue thickness, when knob rotates to specific number, cut the thickness of glue with centimetre
It is corresponding with the number for linear module., can be at 0.05 centimetre by the knob in rotating handle --- it is adjusted in 1 cm range
The rubber knife cuts glue thickness.
When cutting glue, gel to be cut is laid flat (using the DNA running gel of fluorescent dyeing should be placed on ultraviolet gel at
As in system), the gel rubber knife handle is upwards, cutter head lets droop vertical cut glue.
Detailed description of the invention
Fig. 1 is gel rubber knife front elevation disclosed in the invention patent, shows knife handle, cutter head and cuts glue thickness adjustment knob
Position and actual size.
Fig. 2 is the gel rubber knife front elevation of the invention patent description.
Fig. 3 is the gel rubber knife side view of the invention patent description.
Fig. 4 is the holding handle cross-sectional view for another side handle of package that the handle side of fixed U-shaped blade contains.
Fig. 5 be the invention patent description gel rubber knife from cutter head to figure from knife handle direction, with show U-shaped blade and
The relative position of I type blade.
Specific embodiment
Implement to be the limitation to further explanation of the invention, rather than to the technology of the present invention parameter below.
Embodiment 1
Horizontal strip electrophoresis, 120 V pressure stabilizing electricity are carried out using the gel of 1.5% concentration to the pcr amplification product that length is 420 bp or so
Swimming observed expanding effect after 30 minutes in gel imaging system, and the gel rubber knife then described using the present invention is to purpose item
Band carry out gel extraction, cut glue thickness and be adjusted to 0.2 centimetre, can finally obtain complete length be 0.7 centimetre, with a thickness of
0.2 centimetre of recycling blob of viscose, the blob of viscose of the size, which is conveniently put into 1.5 mL PE centrifuge tubes, carries out subsequent colloidal sol and DNA purifying
Processing.
Claims (4)
1. one kind can adjust the gel rubber knife of glue thickness, it is characterized in that: the rubber knife material is 316 stainless steels, by handle, knife
Head is formed with glue thickness adjustment knob is cut.
2. the gel rubber knife of glue thickness can be adjusted described in claims 1, it is characterised in that: handle is V-type bifurcated knot
Structure, shape is similar with surgical forceps, and handle side end installs U-shaped blade；Other side end is installed by I type blade；It is intermediate
The handle side of fixed U-shaped blade contains the holding handle for wrapping up another side handle.
3. the gel rubber knife of glue thickness can be adjusted described in claims 1, it is characterised in that: cutter head is by U-shaped blade and I
Type blade is constituted, and U-shaped blade two sides blade lengths are 1.5 centimetres, and middle single-slice length is 0.72 centimetre；I type blade lengths are
0.7 centimetre.
4. the gel rubber knife of glue thickness can be adjusted described in claims 1, it is characterised in that: cut glue thickness adjustment knob
It is made of the screw being fixed on handle and the knob for being adjusted by rotation handle bifurcated size, wherein indicates instruction on screw and cut glue
The number of thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811558622.0A CN109465861A (en) | 2018-12-19 | 2018-12-19 | A kind of gel rubber knife adjusting glue thickness |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811558622.0A CN109465861A (en) | 2018-12-19 | 2018-12-19 | A kind of gel rubber knife adjusting glue thickness |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109465861A true CN109465861A (en) | 2019-03-15 |
Family
ID=65675163
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811558622.0A Pending CN109465861A (en) | 2018-12-19 | 2018-12-19 | A kind of gel rubber knife adjusting glue thickness |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109465861A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6216348B1 (en) * | 1998-10-14 | 2001-04-17 | Vagan Martirossian | Tool handle |
| DE20209726U1 (en) * | 2002-06-22 | 2002-10-10 | Mühlebach, Moritz, Dietlikon | Device for edging material webs |
| CN1880031A (en) * | 2005-06-16 | 2006-12-20 | 王冠红 | Tool for machining strip-like food |
| DE202008001048U1 (en) * | 2008-01-24 | 2008-04-03 | Uyar, Ufuk | Knife for kebab |
| CN202224175U (en) * | 2011-10-17 | 2012-05-23 | 蔺涛 | Quick gel cutter |
| US20130333534A1 (en) * | 2012-06-19 | 2013-12-19 | Earl Votolato | Safety Knife Handle With Wire Blade |
| CN203524435U (en) * | 2013-11-20 | 2014-04-09 | 浙江商业职业技术学院 | Double-edged knife for removing mango stone |
| CN205614718U (en) * | 2016-05-09 | 2016-10-05 | 河南科技大学 | Negative -pressure adsorption type gel exciser ware |
| CN206048286U (en) * | 2016-09-19 | 2017-03-29 | 中国科学院天津工业生物技术研究所 | A kind of new type gel rubber knife |
| CN206154360U (en) * | 2016-07-05 | 2017-05-10 | 广西师范大学 | Ware is surely glued to running gel of adjustable blade interval |
| CN207104978U (en) * | 2017-04-19 | 2018-03-16 | 新疆维吾尔自治区疾病预防控制中心 | A kind of Double-cutter head pathologic sampling device |
| CN209224122U (en) * | 2018-12-19 | 2019-08-09 | 广东美立康生物科技有限公司 | A kind of gel rubber knife adjusting glue thickness |
-
2018
- 2018-12-19 CN CN201811558622.0A patent/CN109465861A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6216348B1 (en) * | 1998-10-14 | 2001-04-17 | Vagan Martirossian | Tool handle |
| DE20209726U1 (en) * | 2002-06-22 | 2002-10-10 | Mühlebach, Moritz, Dietlikon | Device for edging material webs |
| CN1880031A (en) * | 2005-06-16 | 2006-12-20 | 王冠红 | Tool for machining strip-like food |
| DE202008001048U1 (en) * | 2008-01-24 | 2008-04-03 | Uyar, Ufuk | Knife for kebab |
| CN202224175U (en) * | 2011-10-17 | 2012-05-23 | 蔺涛 | Quick gel cutter |
| US20130333534A1 (en) * | 2012-06-19 | 2013-12-19 | Earl Votolato | Safety Knife Handle With Wire Blade |
| CN203524435U (en) * | 2013-11-20 | 2014-04-09 | 浙江商业职业技术学院 | Double-edged knife for removing mango stone |
| CN205614718U (en) * | 2016-05-09 | 2016-10-05 | 河南科技大学 | Negative -pressure adsorption type gel exciser ware |
| CN206154360U (en) * | 2016-07-05 | 2017-05-10 | 广西师范大学 | Ware is surely glued to running gel of adjustable blade interval |
| CN206048286U (en) * | 2016-09-19 | 2017-03-29 | 中国科学院天津工业生物技术研究所 | A kind of new type gel rubber knife |
| CN207104978U (en) * | 2017-04-19 | 2018-03-16 | 新疆维吾尔自治区疾病预防控制中心 | A kind of Double-cutter head pathologic sampling device |
| CN209224122U (en) * | 2018-12-19 | 2019-08-09 | 广东美立康生物科技有限公司 | A kind of gel rubber knife adjusting glue thickness |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |