CN109464706A - A kind of anti-aging face filler and preparation method thereof based on umbilical cord mesenchymal stem cells - Google Patents
A kind of anti-aging face filler and preparation method thereof based on umbilical cord mesenchymal stem cells Download PDFInfo
- Publication number
- CN109464706A CN109464706A CN201811560147.0A CN201811560147A CN109464706A CN 109464706 A CN109464706 A CN 109464706A CN 201811560147 A CN201811560147 A CN 201811560147A CN 109464706 A CN109464706 A CN 109464706A
- Authority
- CN
- China
- Prior art keywords
- stem cells
- umbilical cord
- mesenchymal stem
- cord mesenchymal
- cellulose
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3616—Blood, e.g. platelet-rich plasma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3834—Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
Abstract
The invention discloses a kind of anti-aging face filler and preparation method thereof based on umbilical cord mesenchymal stem cells, including l-lactic acid, collagen, umbilical cord mesenchymal stem cells, platelet rich plasma and combined gels matrix.The beneficial effects of the present invention are: the content of umbilical cord mesenchymal stem cells is 2 × 106~5 × 106/ml, various kinds of cell growth factor can be secreted, there is promotion cell turnover, promote damaged cell tissue healing, increase cell viability and skin immunity, the effects of beautifying and anti-aging, cellulose ethers in combined gels matrix are methylcellulose, sodium carboxymethylcellulose, ethyl cellulose, hydroxyethyl cellulose, the native celluloses structure such as hydroxypropyl cellulose and hypromellose, it is with excellent biocompatibility, persistence, high moisturizing power, facial filler is set to keep the desired mechanical and rheological equationm of state.
Description
Technical field
The present invention relates to a kind of anti-aging face filler, specially a kind of anti-aging based on umbilical cord mesenchymal stem cells
Facial filler and preparation method thereof, belongs to field of biotechnology.
Background technique
Umbilical cord mesenchymal stem cells, which refer to, is present in one of neonatal umbilical cord tissue versatile stem cell, it can break up
At many kinds of histocytes, there is wide potential applicability in clinical practice, umbilical cord mesenchymal stem cells differentiation potential with higher can
Broken up to multiple directions.It is in organizational projects sides such as bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium and cardiac muscles
Face has wide potential applicability in clinical practice.It has been reported that and isolates mescenchymal stem cell from people's umbilical cord, and cell content, proliferation energy
Power is better than mesenchymal stem cell, and immunogenicity is lower than mesenchymal stem cell, and has materials conveniently, no ethics
The advantages that dispute, therefore increasingly by the concern of research workers.
For existing beauty industry, a variety of wrinkles can be efficiently solved by injecting hyaluronic acid mostly, are reached
The effect for anti-aging of wrinkling, but after injection wave uric acid, sodium hyaluronate can be disappeared by slowly metabolism, and generally speaking its effect can only probably be tieed up
It holds 4-6 months, and everyone is different for the speed of specific Absorption And Metabolism, it is however generally that, it is absorbed after the partially thin injection of constitution
The partially fat people of speed than bulk matter is fast, and additionally, due in skin with age, contained sodium hyaluronate itself is with regard to low, after injection
Relatively young skin is metabolized with regard to very fast, the retention time also can be more shorter, and currently, there has been no utilize umbilical cord mesenchymal stem cells
Skin anti-aging reparation beauty product.
Summary of the invention
The object of the invention is that provide a kind of anti-based on umbilical cord mesenchymal stem cells to solve the above-mentioned problems
Aging face filler and preparation method thereof.
The present invention is through the following technical solutions to achieve the above objectives: a kind of anti-aging based on umbilical cord mesenchymal stem cells
Facial filler and preparation method thereof, including filler include l-lactic acid, collagen, umbilical cord mesenchymal stem cells, richness
Thrombocyte plasma and combined gels matrix；
Wherein, the combined gels matrix includes aqueous solution for injection, cellulose ethers and Sodium Hyaluronate, the cellulose ether
Class accounts for the 10% of the weight total amount of aqueous solution for injection, and the Sodium Hyaluronate accounts for the 3% of the weight total amount of aqueous solution for injection.
Preferably, in order to preferable anti-aging beauty-care effect, the l-lactic acid, collagen and rich platelet
The volume ratio of blood plasma is 1:1:1.
Preferably, in order to which the umbilical cord mesenchyma for isolating high-purity in the short time from complicated cell tissue mixture is dry
Cell, the umbilical cord mesenchymal stem cells are separated using the immunomagnetic beads method based on immuno absorbence principle.
Preferably, it in order to promote damaged cell tissue healing, increase cell viability and skin immunity, is filled between the umbilical cord
The content of matter stem cell is 2 × 106~5 × 106A/ml.
Preferably, in order to make facial filler keep the desired mechanical and rheological equationm of state, in the combined gels matrix
Cellulose ethers be methylcellulose, sodium carboxymethylcellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose and
The native celluloses structure such as hypromellose.
A kind of preparation method of the anti-aging face filler based on umbilical cord mesenchymal stem cells, preparation method include with
Lower step
Step A prepares umbilical cord mesenchymal stem cells, collects bleeding of the umbilicus, after baby due, by having the medical staff of professional qualification
Bleeding of the umbilicus is collected using the sealed blood bag containing anti-coagulants, it is thin to reapply the single core of density-gradient centrifugation method separating umbilical blood
Born of the same parents, and specifically being marked, after magnetic mark is complete, sorting that these cells are placed in strong stabilizing magnetic field by one
Column sub-elects candidate stem cell using immunomagnetic beads method from Streptococcus；
Step B prepares platelet rich plasma, and venous puncture whole blood, centrifugation is centrifuged 10min with 1600r/min for the first time, abandons red
Cellular layer；Second of centrifugation is centrifuged 5min with 5000r/min, inhales after centrifugation and abandons part blood plasma, with remaining 1ml or so blood plasma weight
Outstanding precipitating, obtains platelet rich plasma；
Step C prepares combined gels matrix, it is water-soluble that injection is added in cellulose ethers and Sodium Hyaluronate under slow stirring
In liquid, sufficiently wetting swelling stands 6h, obtains combined gels matrix；
The umbilical cord mesenchymal stem cells of preparation, platelet rich plasma are added to combined gels Medium Culture, and add a left side by step D
Rotation polylactic acid is uniformly mixed with collagen, and product is made.
The beneficial effects of the present invention are: should be based on the anti-aging face filler of umbilical cord mesenchymal stem cells and its preparation side
Rationally, the volume ratio of l-lactic acid, collagen and platelet rich plasma is 1:1:1 for method design, makes made anti-aging
Facial each component content of filler is impartial, and then has preferable anti-aging beauty-care effect, and umbilical cord mesenchymal stem cells use base
It is separated in the immunomagnetic beads method of immuno absorbence principle, does not need to be equipped with large-scale equipment, easy to operate, quick, short time
The interior umbilical cord mesenchymal stem cells that high-purity can be isolated from complicated cell tissue mixture, umbilical cord mesenchymal stem cells
Content be 2 × 106~5 × 106A/ml can secrete various kinds of cell growth factor, have and promote cell turnover, promote damaged cell
Organization healing increases the effects of cell viability and skin immunity, beautifying and anti-aging, the cellulose ethers in combined gels matrix
It is fine for methylcellulose, sodium carboxymethylcellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose and hydroxypropyl
The native celluloses structures such as element are tieed up, with excellent biocompatibility, persistence, high moisturizing power keeps facial filler
The desired mechanical and rheological equationm of state.
Detailed description of the invention
Fig. 1 is present component structural schematic diagram；
Fig. 2 is preparation flow structural schematic diagram of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, a kind of anti-aging face filler based on umbilical cord mesenchymal stem cells, filler includes left-handed poly-
Lactic acid, collagen, umbilical cord mesenchymal stem cells, platelet rich plasma and combined gels matrix；
Wherein, the combined gels matrix includes aqueous solution for injection, cellulose ethers and Sodium Hyaluronate, the cellulose ether
Class accounts for the 10% of the weight total amount of aqueous solution for injection, and the Sodium Hyaluronate accounts for the 3% of the weight total amount of aqueous solution for injection.
The volume ratio of the l-lactic acid, collagen and platelet rich plasma is 1:1:1, is made made anti-ageing
Each component content of old face filler is impartial, and then has preferable anti-aging beauty-care effect, the umbilical cord mesenchymal stem cells
It is separated using the immunomagnetic beads method based on immuno absorbence principle, the equipment for not needing outfit large size, easy to operate, quick,
The umbilical cord mesenchymal stem cells of high-purity can be isolated in short time from complicated cell tissue mixture, between the umbilical cord
The content of mesenchymal stem cells is 2 × 106~5 × 106A/ml can secrete various kinds of cell growth factor, have and promote cell turnover, promote
Into damaged cell tissue healing, increase the effects of cell viability and skin immunity, beautifying and anti-aging, the combined gels matrix
In cellulose ethers be methylcellulose, sodium carboxymethylcellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxy propyl cellulose
The native celluloses structure such as element and hypromellose, with excellent biocompatibility, persistence, high moisturizing power makes
Facial filler keeps the desired mechanical and rheological equationm of state.
A kind of preparation method of the anti-aging face filler based on umbilical cord mesenchymal stem cells, preparation method include with
Lower step
Step A prepares umbilical cord mesenchymal stem cells, collects bleeding of the umbilicus, after baby due, by having the medical staff of professional qualification
Bleeding of the umbilicus is collected using the sealed blood bag containing anti-coagulants, it is thin to reapply the single core of density-gradient centrifugation method separating umbilical blood
Born of the same parents, and specifically being marked, after magnetic mark is complete, sorting that these cells are placed in strong stabilizing magnetic field by one
Column sub-elects candidate stem cell using immunomagnetic beads method from Streptococcus；
Step B prepares platelet rich plasma, and venous puncture whole blood, centrifugation is centrifuged 10min with 1600r/min for the first time, abandons red
Cellular layer；Second of centrifugation is centrifuged 5min with 5000r/min, inhales after centrifugation and abandons part blood plasma, with remaining 1ml or so blood plasma weight
Outstanding precipitating, obtains platelet rich plasma；
Step C prepares combined gels matrix, it is water-soluble that injection is added in cellulose ethers and Sodium Hyaluronate under slow stirring
In liquid, sufficiently wetting swelling stands 6h, obtains combined gels matrix；
The umbilical cord mesenchymal stem cells of preparation, platelet rich plasma are added to combined gels Medium Culture, and add a left side by step D
Rotation polylactic acid is uniformly mixed with collagen, and product is made.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (6)
1. a kind of anti-aging face filler based on umbilical cord mesenchymal stem cells, it is characterised in that: filler includes left-handed poly-
Lactic acid, collagen, umbilical cord mesenchymal stem cells, platelet rich plasma and combined gels matrix；
Wherein, the combined gels matrix includes aqueous solution for injection, cellulose ethers and Sodium Hyaluronate, the cellulose ether
Class accounts for the 10% of the weight total amount of aqueous solution for injection, and the Sodium Hyaluronate accounts for the 3% of the weight total amount of aqueous solution for injection.
2. a kind of anti-aging face filler based on umbilical cord mesenchymal stem cells according to claim 1, feature exist
In: the volume ratio of the l-lactic acid, collagen and platelet rich plasma is 1:1:1.
3. a kind of anti-aging face filler based on umbilical cord mesenchymal stem cells according to claim 1, feature exist
In: the umbilical cord mesenchymal stem cells are separated using the immunomagnetic beads method based on immuno absorbence principle.
4. a kind of anti-aging face filler based on umbilical cord mesenchymal stem cells according to claim 1, feature exist
In: the content of the umbilical cord mesenchymal stem cells is 2 × 106~5 × 106A/ml.
5. a kind of anti-aging face filler based on umbilical cord mesenchymal stem cells according to claim 1, feature exist
In: the cellulose ethers in the combined gels matrix are methylcellulose, sodium carboxymethylcellulose, ethyl cellulose, hydroxyl second
The native celluloses structure such as base cellulose, hydroxypropyl cellulose and hypromellose.
6. a kind of preparation method of the anti-aging face filler based on umbilical cord mesenchymal stem cells, which is characterized in that it is prepared
Method the following steps are included:
Step A prepares umbilical cord mesenchymal stem cells, collects bleeding of the umbilicus, after baby due, by having the medical staff of professional qualification
Bleeding of the umbilicus is collected using the sealed blood bag containing anti-coagulants, it is thin to reapply the single core of density-gradient centrifugation method separating umbilical blood
Born of the same parents, and specifically being marked, after magnetic mark is complete, sorting that these cells are placed in strong stabilizing magnetic field by one
Column sub-elects candidate stem cell using immunomagnetic beads method from Streptococcus；
Step B prepares platelet rich plasma, and venous puncture whole blood, centrifugation is centrifuged 10min with 1600r/min for the first time, abandons red
Cellular layer；Second of centrifugation is centrifuged 5min with 5000r/min, inhales after centrifugation and abandons part blood plasma, with remaining 1ml or so blood plasma weight
Outstanding precipitating, obtains platelet rich plasma；
Step C prepares combined gels matrix, it is water-soluble that injection is added in cellulose ethers and Sodium Hyaluronate under slow stirring
In liquid, sufficiently wetting swelling stands 6h, obtains combined gels matrix；
The umbilical cord mesenchymal stem cells of preparation, platelet rich plasma are added to combined gels Medium Culture, and add a left side by step D
Rotation polylactic acid is uniformly mixed with collagen, and product is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811560147.0A CN109464706A (en) | 2018-12-20 | 2018-12-20 | A kind of anti-aging face filler and preparation method thereof based on umbilical cord mesenchymal stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811560147.0A CN109464706A (en) | 2018-12-20 | 2018-12-20 | A kind of anti-aging face filler and preparation method thereof based on umbilical cord mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109464706A true CN109464706A (en) | 2019-03-15 |
Family
ID=65675189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811560147.0A Withdrawn CN109464706A (en) | 2018-12-20 | 2018-12-20 | A kind of anti-aging face filler and preparation method thereof based on umbilical cord mesenchymal stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109464706A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109966555A (en) * | 2019-05-06 | 2019-07-05 | 常州立新医疗美容诊所有限公司 | A kind of elastic cell's modeling face facial bulking agent compositions gel and preparation method thereof |
| CN112402364A (en) * | 2020-10-23 | 2021-02-26 | 中科细胞科技(广州)有限公司 | Umbilical cord mesenchymal stem cell-platelet-rich plasma-containing composite repair gel for injection |
-
2018
- 2018-12-20 CN CN201811560147.0A patent/CN109464706A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109966555A (en) * | 2019-05-06 | 2019-07-05 | 常州立新医疗美容诊所有限公司 | A kind of elastic cell's modeling face facial bulking agent compositions gel and preparation method thereof |
| CN112402364A (en) * | 2020-10-23 | 2021-02-26 | 中科细胞科技(广州)有限公司 | Umbilical cord mesenchymal stem cell-platelet-rich plasma-containing composite repair gel for injection |
| CN112402364B (en) * | 2020-10-23 | 2023-08-04 | 中科细胞科技(广州)有限公司 | Umbilical cord mesenchymal stem cell-platelet-rich plasma-containing composite repair gel for injection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107854418A (en) | A kind of human umbilical cord mesenchymal stem cells excretion external use creams and preparation method thereof | |
| CN107245472A (en) | A kind of preparation method, the application method of human mesenchymal stem cell excretion body freeze-dried powder | |
| CN102058905B (en) | Composite fat granule and preparation method thereof | |
| CN109464706A (en) | A kind of anti-aging face filler and preparation method thereof based on umbilical cord mesenchymal stem cells | |
| CN105985925B (en) | A kind of global function artificial organs fitting body and its preparation and cultural method | |
| CN106823002A (en) | A kind of anti-aging face filler and preparation method thereof | |
| CN112239746A (en) | Preparation method of exosome extract of human umbilical cord mesenchymal stem cells and preparation method of exosome cream | |
| CN103071191A (en) | Preparation method of autologous platelet-factor-rich plasma (PFRP) preparation | |
| CN105030647B (en) | A kind of preparation for reducing wrinkle and preparation method thereof | |
| KR20160024881A (en) | Lipofilling with ex-vivo expanded adipose tissue-derived stem cells for cosmetic breast filling or for facial filling and/or rejuvenation | |
| Korurer et al. | Production of a composite hyaluronic acid/gelatin blood plasma gel for hydrogel‐based adipose tissue engineering applications | |
| CN107929809A (en) | A kind of Acelluar small intestinal submucosa matrix particles of injectable and preparation method and application | |
| CN105536064B (en) | A kind of compound soft tissue repair hydrogel and its preparation method and application | |
| CN109010921A (en) | A kind of bionic joint cartilage composite hydrogel and preparation method thereof with gradient rigidity | |
| CN107496324A (en) | A kind of anti-aging face filler based on umbilical cord mesenchymal stem cells and preparation method thereof | |
| CN104490727A (en) | Composition of stem cells and hyaluronic acid and application of composition | |
| CN109966555A (en) | A kind of elastic cell's modeling face facial bulking agent compositions gel and preparation method thereof | |
| JP5320501B1 (en) | Carrier for transporting cell sheets for transplantation | |
| US20160206551A1 (en) | Methods for the repair and rejuvenation of tissues using platelet-rich plasma compositions | |
| CN106540334A (en) | A kind of compositionss and its application | |
| CN109504655A (en) | A kind of method of the separation and secondary culture of fat stem cell | |
| CN105483077A (en) | Cell culture method for improving hepatocyte proliferation activity | |
| CN102585276B (en) | Method for preparing porous chitosan scaffold | |
| CN115651277A (en) | Preparation method of carbon quantum dot modified hyaluronic acid and food preparation | |
| CN108715833A (en) | A kind of method for preparing microsphere of load platelet lysates liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190315 |