CN109455745A - A kind of method and product and application preparing modified Nano MgO using rosemary flower extracting solution - Google Patents

A kind of method and product and application preparing modified Nano MgO using rosemary flower extracting solution Download PDF

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CN109455745A
CN109455745A CN201811339719.2A CN201811339719A CN109455745A CN 109455745 A CN109455745 A CN 109455745A CN 201811339719 A CN201811339719 A CN 201811339719A CN 109455745 A CN109455745 A CN 109455745A
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mgo
rosemary
modified nano
extracting solution
solution
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洪纤纤
李斌
吴秀芹
邱文
张阳
亚斯明·阿卜杜拉
索拉.奥莱坦
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Zhejiang University ZJU
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    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01F—COMPOUNDS OF THE METALS BERYLLIUM, MAGNESIUM, ALUMINIUM, CALCIUM, STRONTIUM, BARIUM, RADIUM, THORIUM, OR OF THE RARE-EARTH METALS
    • C01F5/00—Compounds of magnesium
    • C01F5/02—Magnesia
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2002/00—Crystal-structural characteristics
    • C01P2002/70—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data
    • C01P2002/72—Crystal-structural characteristics defined by measured X-ray, neutron or electron diffraction data by d-values or two theta-values, e.g. as X-ray diagram
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2002/00—Crystal-structural characteristics
    • C01P2002/80—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70
    • C01P2002/82—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70 by IR- or Raman-data
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2002/00—Crystal-structural characteristics
    • C01P2002/80—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70
    • C01P2002/84—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70 by UV- or VIS- data
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2002/00—Crystal-structural characteristics
    • C01P2002/80—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70
    • C01P2002/85—Crystal-structural characteristics defined by measured data other than those specified in group C01P2002/70 by XPS, EDX or EDAX data
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/01—Particle morphology depicted by an image
    • C01P2004/03—Particle morphology depicted by an image obtained by SEM
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/01—Particle morphology depicted by an image
    • C01P2004/04—Particle morphology depicted by an image obtained by TEM, STEM, STM or AFM
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/20—Particle morphology extending in two dimensions, e.g. plate-like
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/60—Particles characterised by their size
    • C01P2004/64—Nanometer sized, i.e. from 1-100 nanometer

Abstract

The invention discloses a kind of methods for preparing modified Nano MgO using rosemary flower extracting solution, include the following steps: to pulverize after rosemary 1) is spent clean, drying, obtained rosemary pollen end is dissolved in deionized water again, it dissolves by heating, is cooling, after filtering, obtaining rosemary flower extracting solution；2) it is heated after under agitation, rosemary flower extracting solution is mixed with MgO solution, is centrifuged, washs, obtaining modified Nano MgO after vacuum freeze drying.The simple and quick green of the method for the present invention synthetic method, material are easy to obtain, and obtained modified Nano MgO product has the effect of significantly inhibiting rice leaf spot bacteria, can be as the bacteriostatic agent of the important plant diseases such as prevention and treatment rice bacterial leaf spot.

Description

It is a kind of using rosemary flower extracting solution prepare modified Nano MgO method and product and Using
Technical field
The present invention relates to the preparation fields of nano material, and in particular to a kind of to prepare modified receive using rosemary flower extracting solution The method and product of rice MgO and application.
Background technique
Nano-MgO has the characteristics that, large specific surface area nontoxic to environment, magnetic strong, absorption and scatters ultraviolet ability are strong, Compared with common MgO, chemical industry, electronics, biology, in terms of fully demonstrate its huge application value.
For nano-MgO as a kind of anti-biotic material, the active oxygen (ROS) generated may result in the release of oxidative stress, And then lead to the destruction of cell membrane.Nano-MgO is small compared to common MgO size, large specific surface area, and the release of surface ion can It is come into full contact with bacterial cell membrane, leads to the permeabilization of film, be destroyed its structure with function, ultimately cause cell content logistics It loses, cell death.Therefore nano-MgO has broad spectrum activity, can kill most of virus, bacterium and work well, and has relatively wide Wealthy application potential.
And traditional nano-MgO mainly passes through vapor phase method, liquid phase method, solid phase method preparation, and there is high pollution, highly energy-consuming etc. to lack Point first prepares organic emulsion, then make if Patent Application Publication CN105271319A discloses a kind of method for preparing nano-MgO Standby compound, finally roasts compound；Patent Application Publication CN104445296A discloses a kind of spherical shape MgO nanometers The synthetic method of grain, using magnesium salts, acetamide as raw material, polyethylene glycol is soft template, and chela occurs during temperature constant magnetic stirring Synthesizing chemical-reacting.
But with the development of science and technology the production of the following nano materials technology must will towards low cost, low consumption, Low pollution development, has the research using plant extraction liquid synthesis nano metal material in the prior art, and cardinal principle is to plant Substance such as alkaloid, phenolic compound, terpenoid and coenzyme in object material can be used as reducing agent and stabilizer preparation is received Rice corpuscles.
Summary of the invention
It is an object of that present invention to provide a kind of method for preparing modified Nano MgO using rosemary flower extracting solution, the systems Standby simple process, it is environmentally protective, can be mass-produced, obtained modified Nano MgO has good fungistatic effect, can be used as water Rice antibacterial agent uses.
The purpose of the present invention is achieved through the following technical solutions:
A method of modified Nano MgO being prepared using rosemary flower extracting solution, is included the following steps:
(1) it pulverizes after rosemary being spent clean, drying, then obtained rosemary pollen end is dissolved in deionized water, It dissolves by heating, is cooling, after filtering, obtaining rosemary flower extracting solution；
(2) under agitation, it is heated after the rosemary flower extracting solution that step (1) obtains being mixed with MgO solution, Modified Nano MgO is obtained after centrifugation, washing, vacuum freeze drying.
The present invention chooses rosemary and spends rosemary flower extracting solution obtained to Escherichia coli, staphylococcus aureus, withered grass Bacillus, saccharomycete, fungi have good inhibitory activity, have extensive, good anti-microbial effect, are modified with it MgO solution, a kind of available modified Nano MgO material with extremely strong fungistatic effect and stable structure.
In step (1), the temperature of the heating is 50~90 DEG C, and heating time is 3~7h, the temperature of heating is too low, when Between too short to will lead to synthetic effect bad；Heating temperature is excessively high, overlong time will cause the unnecessary wasting of resources.
In step (1), the volume ratio of the powder from rosemary and deionized water is 1:50~200, rosemary flower dosage mistake Height will lead to the unnecessary waste of vegetable material, and too low be equivalent to of rosemary flower dosage reduces rosemary extract concentration, meeting Cause the nano antibacterial agent finally synthesized ineffective.
In step (2), the concentration of the MgO solution is 0.1~10mM, and concentration is too low to be not enough to synthesize nano-MgO, concentration It is excessively high, it not only influences the stability of product structure and also will cause the waste of MgO.
In step (2), the volume ratio of the rosemary extracting solution and MgO solution is 2~4:5, rosemary extracting solution dosage Too low to will lead to that effective antipathogenic composition therein is few, the nano material fungistatic effect of synthesis reduces.
In step (2), the rate of the stirring is 400~800rpm/min, too low meeting of stirring rate so that extracting solution and The contact of MgO solution is insufficient, reaction is not enough, and stirring rate is excessively high, may destroy product structure and cause unnecessary money Source waste.
In step (2), the centrifugal rotational speed is 3000~8000rpm/min, and centrifugation time is 10~20min, centrifugation speed Rate is too low, the time is too short, will lead to supernatant and not enough clarifies, and the nanometer powder finally obtained is impure.
The invention also discloses the modified Nano MgO according to made from the above method, the average grain diameter of the modified Nano MgO For 7~20nm.
The invention also discloses application of the above-mentioned modified Nano MgO in prevention and treatment bacterial blight of rice, first by modified Nano MgO is dissolved in water and modified Nano MgO solution is made, then is uniformly sprayed on modified Nano MgO is molten on rice seedlings.
The present invention has studied modified Nano MgO to the antibacterial effect for the rice leaf spot bacteria for currently causing China seriously to occur Fruit, it has significant inhibitory effect to for examination pathogen for creative having found, obtained modified Nano MgO solution is uniformly sprayed It applies on the rice seedlings that may break out bacterial leaf spot disease, effectively bacterial blight of rice evil can be prevented and treated, this is in agricultural There are wide application and promotion prospect in production field.
Compared with the prior art, the present invention has the following beneficial effects:
(1) preparation process of the invention is simple, environmentally protective and cheaply easily acquisition, the energy disappear without catalyst, raw material It consumes relatively fewer, is very suitable for being mass produced；
(2) average grain diameter for the modified Nano MgO that the present invention obtains is small, and fungistatic effect is significant, can kill absolutely mostly The number bacterial leaf-blight original, being applied in prevention and treatment bacterial blight of rice has good control efficiency, is very suitable for leading in agricultural Domain is promoted, and is had broad prospects.
Detailed description of the invention
Fig. 1 is the flow chart that MgONFs is prepared in embodiment 1；
Fig. 2 is UV, visible light (UV-Vis) abosrption spectrogram of MgONFs obtained in embodiment 1；
Fig. 3 is the XRD diagram of MgONFs obtained in embodiment 1；
Fig. 4 schemes for the FTIR of MgONFs obtained in embodiment 1；
Fig. 5 is the TEM microphoto (Fig. 5 A) of MgONFs obtained in embodiment 1；SEM micrograph (Fig. 5 B, C)；EDS Energy spectrum diagram (Fig. 5 D)；
Fig. 6 is that concentration is respectively that MgONFs (figure is made in 4 μ g/ml, 8 μ g/ml, the MgO (Fig. 6 A) of 16 μ g/ml and embodiment 1 6B) to the inhibiting effect comparison diagram of rice leaf spot bacteria；
Fig. 7 is the MgONFs of various concentration made from embodiment 1 to the growth (Fig. 7 A) of rice leaf spot bacteria, biomembrane Form the influence diagram of (Fig. 7 B) and motility (Fig. 7 C)；
Fig. 8 is the TEM photo (Fig. 8 A) of normal water rice bacterial leaf spot pathogenic bacteria and the concentration made from embodiment 1 is 4 μ g/ml MgONFs handle the TEM photo (Fig. 8 B, C, D) of lower bacterium.
Specific embodiment
Combined with specific embodiments below, the present invention is furture elucidated.
Embodiment 1
(1) choose fresh rosemary flower, by cleaning, grind into powder after drying, can vacuum be stored in -10 DEG C of refrigerators In；Obtained rosemary pollen end and deionized water are dissolved in 1:100 ratio again and mixed, 4h, room are heated at high temperature at 70 DEG C After temperature is cooling twice with filter paper filtering, filtrate is finally collected, filemot rosemary extracting solution is obtained；
(2) by volume it is that 1:1 is mixed with the MgO solution of 1mM by rosemary extracting solution, is 600rpm/ in rate Under the conditions of the magnetic stirring apparatus of min, it is heated to 70 DEG C and continuously stirs 4h；It is centrifuged 15min, centrifugal rotational speed 5000rpm/min again, Precipitating is taken, after being washed with deionized twice, powdered granule, as modified Nano MgO are prepared into using vacuum freeze-drying method (MgONFs)。
Fig. 1 is the flow chart that MgONFs is prepared in the present invention, is first respectively configured to MgO powder and rosemary pollen end Solution, the two carry out heating stirring in certain proportion, pass through freeze-drying skill after filemot MgONFs extracting solution to be formed Art is prepared into nanometer powder.
Using ultraviolet-visible absorption spectroscopy (UV-VIS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), The physicochemical property and structure feature of transmission electron microscope (TEM), scanning electron microscope (SEM) evaluation MgONFs are specific to tie Fruit is as follows:
(1) UV-VIS spectrometer is judged in solution mixture by absorption value with the presence or absence of nano particle and its structure table Sign.
UV, visible light (UV-Vis) abosrption spectrogram for the MgONFs that Fig. 2 is, as shown in Figure 2, in 200-800nm model There are two absorption peaks in enclosing, and wherein maximum absorption band is at 250nm.
(2) X-ray diffraction (XRD) is met and get along well by X-ray and crystal phase occurs diffraction phenomena as basic principle, becomes research The effective ways of crystal and certain non-crystalline material microstructures, can perform well in the research of crystal structure, reflect crystal Purity and size, the higher and narrow diffraction maximum of peak value show the formation of the small size nanometer magnesium crystal of high-crystallinity.
The XRD diagram for the MgONFs that Fig. 3 is, from the figure 3, it may be seen that at 38.048 °, 41.095 °, 62.342 °, 78.628 ° Diffraction maximum respectively corresponds (100) (101) (102) and (202) type nanometer Mg crystal face and diffraction maximum is sharp, passes through Debye- The average grain diameter that MgONFs is calculated in Scherrer formula is 8.8nm.
(3) infrared absorption of Fourier transform infrared spectroscopy (FTIR) reflection intramolecular difference chemical group, can be used for reflecting Determine nanometer Mg biomolecule that may be present, using Fourier transform infrared spectroscopy (FTIR) measurement MgONFs molecule deformation and Stretching vibration frequency.
The FTIR for the MgONFs that Fig. 4 is schemes, as shown in Figure 4,3405cm-1Left and right is the stretching vibration band of water, 1647cm-1Left and right is the deformation vibration band of water, 430cm-1Place is Mg-O key chattering absorption band.
(4) Asia less than 0.2 μm that can not be seen clearly under an optical microscope can be seen using transmission electron microscope (TEM) Microstructure or ultra microstructure, the TEM microphoto for the MgONFs that Fig. 5 A is, as seen from the figure, obtained MgONFs shape Looks keep good；
Using the various physics of scanning electron microscope (SEM) available sample itself, the information of chemical property, Such as pattern, composition, crystal structure can be used for observing shape, the size of the nanometer magnesium granules synthesized in the present invention, Fig. 5 B, C For MgONFs SEM micrograph obtained, by Fig. 5 B it is found that MgONFs due to specific surface area with higher and surface can and Easily reunite, by Fig. 5 C it is found that MgONFs is flaky；
EDS power spectrum image can obtain the essential informations such as the form, size, material composition of nano material, and Fig. 5 D is obtained The EDS energy spectrum diagram of MgONFs only has the presence of Mg and O element, shows the presence of nano-MgO as seen from the figure in sample.
The MgONFs of different quality is dissolved in deionized water, and to be configured to 4 μ g/ml, 8 μ g/ml, the MgONFs of 16 μ g/ml molten Liquid, then by the growth of measurement rice leaf spot bacteria, biofilm formation, motility and observation normally and under nanometer Mg processing Cell membrane TEM microphoto, detects the bacteriostatic activity of MgONFs, and testing result is as follows:
(1) by carrying out MgO and MgONFs respectively to the antagonistic experiment of rice leaf spot bacteria on NA plate, use is antibacterial Circle removes the anti-microbial property of evaluation material.
Fig. 6 is that concentration is respectively 4 μ g/ml, 8 μ g/ml, the MgO (Fig. 6 A) of 16 μ g/ml and MgONFs (Fig. 6 B) white to rice The bacteriostasis comparison diagram of leaf spoting bacteria；It will be appreciated from fig. 6 that inhibition zone of the MgO under 3 various concentrations is all only 0.7cm, and it is dense Degree is that the inhibition zone of the MgONFs solution of 4 μ g/ml, 8 μ g/ml, 16 μ g/ml respectively reaches 3.2cm, 4.4cm and 5.1cm, is seen below Table 1, therefore MgONFs has very strong bacteriostatic activity, it is higher that fungistatic effect is apparently higher than common MgO and MgONFs concentration, suppression Bacterium activity is better.
(2) absorbance under 600nm is measured by ultraviolet specrophotometer, Fig. 7 A is the MgONFs of various concentration to rice The influence diagram of leaf spot bacteria growth can illustrate that MgONFs has very strong bacteriostatic activity, and 16 μ g/ml fungistatic effects are best.
(3) by measurement bacterial biof iotalm formed with travelling ability can indirect assessment MgONFs fungistatic effect, such as Fig. 7 B It is shown, biofilm development is measured by crystal violet staining assay, is compared to normal biofilm, adds 4,8,16 μ of concentration The MgONFs of g/ml, bacterial biof iotalm Forming ability reduce 77.7%, 81.5% and 86.7% respectively, see the table below 1.
The travelling vigor of bacterium is measured on 0.3%NA plate, Fig. 7 C is the MgONFs of various concentration to bacterial blight of rice The motility influence diagram of bacterium, as shown in Figure 7, when MgONFs concentration is 4,8,16 μ g/ml, inhibiting rate is respectively 44.8%, 52.9% and 58.8%, it see the table below 1.
(4) it in order to which intuitive judgment MgONFs is to the extent of the destruction of bacterial biof iotalm, is seen using transmission electron microscope (TEM) The cell wall structure (Fig. 8 A) and 4g/ml MgONFs antibacterial material for examining normal rice leaf spot bacteria handle the cell of lower bacterium Wall (Fig. 8 B, C, D), discovery cell wall damage, cell obviously have content outflow.
Table 1
Embodiment 2
(1) choose fresh rosemary flower, by cleaning, grind into powder after drying, can vacuum be stored in -10 DEG C of refrigerators In；Obtained rosemary pollen end and deionized water are dissolved in 1:200 ratio again and mixed, 3h, room are heated at high temperature at 50 DEG C After temperature is cooling twice with filter paper filtering, filtrate is finally collected, filemot rosemary extracting solution is obtained；
(2) by volume it is that 2:5 is mixed with the MgO solution of 0.1mM by rosemary extracting solution, is in rate Under the conditions of the magnetic stirring apparatus of 400rpm/min, it is heated to 50 DEG C and continuously stirs 4h；It is centrifuged 15min again, centrifugal rotational speed is 3000rpm/min takes precipitating, after being washed with deionized twice, is prepared into powdered granule using vacuum freeze-drying method, as Modified Nano MgO (MgONFs).
Obtained MgONFs is dissolved in deionized water and is configured to 16 μ g/ml solution, measures its life to bacterial blight of rice bacterium Long inhibiting rate is 80.5%, and biofilm formation inhibiting rate is 76.8%, and motility inhibiting rate is 49.3%.
Embodiment 3
(1) choose fresh rosemary flower, by cleaning, grind into powder after drying, can vacuum be stored in -10 DEG C of refrigerators In；Obtained rosemary pollen end and deionized water are dissolved in 1:50 ratio again and mixed, 7h, room temperature are heated at high temperature at 90 DEG C After cooling twice with filter paper filtering, filtrate is finally collected, filemot rosemary extracting solution is obtained；
(2) by volume it is that 4:5 is mixed with the MgO solution of 10mM by rosemary extracting solution, is 800rpm/ in rate Under the conditions of the magnetic stirring apparatus of min, it is heated to 90 DEG C and continuously stirs 4h；It is centrifuged 15min, centrifugal rotational speed 5000rpm/min again, Precipitating is taken, after being washed with deionized twice, powdered granule, as modified Nano MgO are prepared into using vacuum freeze-drying method (MgONFs)。
Obtained MgONFs is dissolved in deionized water and is configured to 16 μ g/ml solution, measures its life to bacterial blight of rice bacterium Long inhibiting rate is 86.5%, and biomembrane inhibiting rate is 80.8%, and motility inhibiting rate is 52.1%.
Application examples
In the greenhouse or field can weigh the MgONFs powder 0.2g that embodiment 1 obtains and be dissolved in 50L water and be configured to 4 μ g/ Ml MgONFs solution is uniformly sprayed anti-there may be on the rice seedlings of bacterial leaf spot disease, can effectively carry out disease to it It controls.

Claims (9)

1. a kind of method for preparing modified Nano MgO using rosemary flower extracting solution, includes the following steps:
(1) it pulverizes after rosemary being spent clean, drying, then obtained rosemary pollen end is dissolved in deionized water, heat After dissolution, cooling, filtering, rosemary flower extracting solution is obtained；
(2) under agitation, it is heated after the rosemary flower extracting solution that step (1) obtains being mixed with MgO solution, from Modified Nano MgO is obtained after the heart, washing, vacuum freeze drying.
2. the temperature of the heating is 50~90 DEG C the method according to claim 1, wherein in step (1), Heating time is 3~7h.
3. the method according to claim 1, wherein in step (1), the rosemary pollen end and deionized water Volume ratio be 1:50~200.
4. the method according to claim 1, wherein the rosemary flower extracting solution and MgO are molten in step (2) 2~4:5 of volume ratio of liquid, the concentration of the MgO solution are 0.1~10mM.
5. the temperature of the heating is 50~90 DEG C the method according to claim 1, wherein in step (2), Heating time is 3~7h.
6. the method according to claim 1, wherein in step (2), the rate of the stirring is 400~ 800rpm/min。
7. the method according to claim 1, wherein in step (2), the centrifugal rotational speed is 3000~ 8000rpm/min, centrifugation time are 10~20min.
8. modified Nano MgO made from a kind of method of any one according to claim 1~7, which is characterized in that the modified Nano The average grain diameter of MgO is 7~20nm.
9. a kind of application of modified Nano MgO according to claim 8 in prevention and treatment bacterial blight of rice, feature exist In specific application method are as follows: the modified Nano MgO is dissolved in water, modified Nano MgO solution is made, then by modified Nano MgO solution is uniformly sprayed on rice seedlings.
CN201811339719.2A 2018-11-12 2018-11-12 A kind of method and product and application preparing modified Nano MgO using rosemary flower extracting solution Pending CN109455745A (en)

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Citations (2)

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Publication number Priority date Publication date Assignee Title
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CN105849203A (en) * 2013-12-19 2016-08-10 3M创新有限公司 Nanoparticle powder composition and method of making the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101563294A (en) * 2006-10-11 2009-10-21 巴斯夫欧洲公司 Method for the production of surface-modified, nanoparticulate metal oxides, metal hydroxides and/or metal oxyhydroxides
CN105849203A (en) * 2013-12-19 2016-08-10 3M创新有限公司 Nanoparticle powder composition and method of making the same

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Title
VARSHA SRIVASTAVA ET AL.: "Green synthesis of magnesium oxide nanoflower and its application for the removal of divalent metallic species from synthetic waste water", 《CERAMICS INTERNATIONAL》 *
广西农业科学院 等: "《土农药——防治水稻病虫害》", 31 May 1976, 广西人民出版社 *
李成祥: "多种植物提取物的抑菌活性研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *
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