CN109444438A - A kind of stabilizer and APTT detection reagent for APTT detection reagent - Google Patents
A kind of stabilizer and APTT detection reagent for APTT detection reagent Download PDFInfo
- Publication number
- CN109444438A CN109444438A CN201811249754.5A CN201811249754A CN109444438A CN 109444438 A CN109444438 A CN 109444438A CN 201811249754 A CN201811249754 A CN 201811249754A CN 109444438 A CN109444438 A CN 109444438A
- Authority
- CN
- China
- Prior art keywords
- reagent
- stabilizer
- detection reagent
- aptt
- aptt detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a kind of stabilizer for APTT detection reagent and APTT detection reagents, belong to technical field of biological.Stabilizer of the invention includes the following components'mass percentage: 55.3%~58.9% chitosan oligosaccharide, 17.2%~20.8% Glucosamine, surplus are auxiliary reagent, and dosage of the stabilizer in the detection reagent for including ellagic acid and rabbit cephalin is 5.0%~5.5%.Stabilizer of the invention is green non-poisonous, and production process is safer, good to the stablizing effect of APTT detection reagent.
Description
Technical field
The invention belongs to technical field of biological, it is related to a kind of stabilizer for APTT detection reagent and APTT detection
Reagent.
Background technique
Human body blood coagulation approach is divided into intrinsic coagulation pathway and exogenous cruor pathway, activated partial thromboplastin time
(APTT) be intrinsic coagulation system an important indicator.APTT extend the reason of be common in factor II, V, VIII,
Ⅸ, Ⅺ, Ⅻ attenuating, afibrinogenemia, Fibrinolytic Activity enhancing, anticoagulant substances exist (in such as blood heparin content increase and
Oral anticoagulant).The reason of APTT shortens is common in hypercoagulative state, (such as myocardial infarction, the unstability heart twist thrombotic diseases
Bitterly, cerebrovascular disease, pulmonary infarction, Deep vain thrombosis, pregnancy-hypertension syndrome and nephrotic syndrome).Therefore by pair
The detection of APTT will be seen that endogenous, coagulation factor in extrinsic coagulation system substantially variation in blood, as fruit part is endogenous
Property deficiency of coagulation factors, will lead to APTT extension；It can be also widely applied to that disease is preoperative, treatment effect of hemorrhagic disease is seen
It examines, is the important indicator of monitor heparin therapy for the monitoring of anticoagulation medicine heparin dosage.
APTT reagent is generally made of activator, phosphatide, bivalent metal ion salt, stabilizer, buffer.Common activation
Agent has diatomite (trade name Celite), white bole, silicon dioxide microparticle, ellagic acid (Ellagic acid) etc..Different portions
Point thromboplastin, different activator and different activationary times to various clotting factor deficiencies, to heparin and to lupus anticoagulant
The sensibility of substance differs greatly, as white bole is most sensitive to coagulation factor, ellagic acid is to Lupus anticoagulant sensibility highest.
Phosphatide is the lipid containing phosphoric acid, belongs to complex liped, and as platelet substitutes, phosphatide participates in intrinsic coagulation pathway.However
Phosphatide easily reacts generation precipitating with ellagic acid, and many reagents all have precipitating currently on the market, and phosphatide is rich in unsaturated fat
Acid is easily oxidized to generate a series of lipid peroxides, to affect its physiological activity.Current detection reagent is often used
Sodium azide is as antiseptic and inhibiting bacteria function agent, however Sodium azide has severe toxicity, and indissoluble solution, and setup time is long, when leading to operator president
Between contact and endanger health.
The principle of APTT detection is that APTT detection reagent is added in test plasma, in calcium ion presence, in activation
Property blood coagulation system in source makes fibrinogen be changed into insoluble fibrin, and measurement test plasma solidifies the required time, as
The activated partial thromboplastin time of test plasma.APTT continuous mode is influenced by many factors, and wherein the quality of reagent is
Most critical factor.The problems such as generally existing unstable product quality of current country's APTT detection reagent, reagent are easy precipitating, causes
Testing result is unstable, and the result measured between different Clinical Test Labs is difficult to compare.Therefore current clinical labororatory
The used reagent overwhelming majority is imported product, but imported product is expensive.
The Glucosamine that chitosan oligosaccharide (also known as glucose oligosaccharide amine, oligo-glucosamine etc.), the i.e. degree of polymerization are 2~10
Polymer, molecular weight≤3200Da are degraded by special biological enzyme technology by chitosan and are generated.Amino and hydroxyl in its molecule
The reactions such as base can be aoxidized, be esterified, is etherified, is acylated, is alkylated, carboxylated, graft copolymerization and crosslinking.Chitosan oligosaccharide has excellent
Good physicochemical property: (1) preferable water-soluble, be easily absorbed by the body；(2) safe and non-toxic；(3) anti-oxidant, antitumor；(4)
The anti-mattress of anti-corrosion；(5) blood lipid and blood glucose are adjusted；(6) enhancing is immune；(7) pH value of human body and activation intestinal flora are adjusted.
Glucosamine is the final hydrolysate of chitin, molecular formula C6H13NO5, it is a kind of natural amino list
One of sugar and the most abundant monosaccharide of nature content, molecular structural formula is as follows.
Glucosamine is the important as precursors in protein or lipid glycosylation, with active physicochemical property and compared with
Strong bioactivity, such as inoxidizability, the expression for improving articular cartilage and synovia molecule, strengthen immunity, antitumor, anti-inflammatory suppression
Bacterium etc..
Summary of the invention
It is a kind of for APTT detection reagent the purpose of the present invention is in view of the above-mentioned problems existing in the prior art, proposing
Stabilizer, the stabilizer are good to the stablizing effect of APTT detection reagent, and do not include the big reagent such as Sodium azide of toxicity, produce
Journey is safer.
Object of the invention can be realized by the following technical scheme:
A kind of stabilizer for APTT detection reagent, the stabilizer include the following components'mass percentage:
Chitosan oligosaccharide 55.3%~58.9%,
Glucosamine 17.2%~20.8%,
Surplus is auxiliary reagent.
The present invention uses the stabilizer of chitosan oligosaccharide, Glucosamine as APTT detection reagent, can effectively improve APTT
The stability and production security of detection reagent.
Contain hydroxyl, amino in chitosan oligosaccharide molecule, easily chemically reacted, as O- is acylated anti-with N- acylation reaction, esterification
It answers, etherification reaction, alkylated reaction, oxidation reaction, graft copolymerization and cross-linking reaction etc., it can be in APTT detection reagent
Function served as bridge is played between active principle, to improve the stability of reagent.The residual sugar base of chitosan oligosaccharide has amino on C2,
There is hydroxyl on C3, from conformation, both for equatorial bond.This structure feature, so that chitosan oligosaccharide in clotting reagent to having
The metal ion of certain ionic radius has chelation, to can avoid preparing lacking of containing in raw material because of APTT detection reagent
The problem that amount heavy metal ion causes reagent unstable.Often contain ellagic acid and rabbit brain in main component in APTT detection reagent
Phosphatide, the two easily react, and generate precipitating, and chitosan oligosaccharide can increase the viscosity and density of reagent, improve the deposited phenomenon.
Glucosamine also has good metal chelation abilities, can play similar EDTA sodium salt or sylvite to metal from
The chelation of son.Glucosamine and chitosan oligosaccharide act synergistically, and can effectively improve the stability of APTT detection reagent.Amino Portugal
Grape sugar also has preferable reducing power and understands the ability of free radical, has good antioxidant action, can effectively protect
Protect APTT detection reagent.
In addition, chitosan oligosaccharide, Glucosamine have certain bacteriostasis.Chitosan oligosaccharide, Glucosamine and auxiliary examination
The synergistic effect of agent can make the Sodium azide for playing the role of antiseptic and inhibiting bacteria function in conventional stabilizer be avoided use, and Sodium azide has play
Poison, and indissoluble solution need the longer configuration processing time, will lead to operator and contact with its long-time and damage body, because
This makes the production of APTT measurement reagent and use process safer.
Preferably, the auxiliary reagent includes the ascorbic acid for accounting for stabilizer mass percent 3.0%~4.5%.
Preferably, the auxiliary reagent includes the Butylated hydroxy for accounting for stabilizer mass percent 0.7%~1.2%
Toluene.
Preferably, the auxiliary reagent includes the mannitol for accounting for stabilizer mass percent 18.7%~19.7%.
Ascorbic acid main function in the present invention is to stablize chitosan oligosaccharide and Glucosamine, prevents it from side reaction occurs.
The main function of butylated hydroxytoluene is the density and viscosity for improving solution, prevents from settling.
In addition, the ascorbic acid, butylated hydroxytoluene itself in the present invention have certain antibacterial antioxidation, sweet dew
Alcohol also has the function of antiseptic and inhibiting bacteria function, and three is used cooperatively with chitosan oligosaccharide, Glucosamine, and stabilizer can be made to prevent with excellent
Rotten bacteriostasis, so as to avoid the use of conventional bacteriostatic preservative Sodium azide completely.
Preferably, the stabilizer includes the following components'mass percentage:
Chitosan oligosaccharide 57.14%,
Glucosamine 19.05%,
Ascorbic acid 3.81%,
Butylated hydroxytoluene 0.95%,
Mannitol 19.05%.
It is described another object of the present invention is to provide a kind of APTT detection reagent using stabilizer as previously described
APTT detection reagent is made of reagent I and reagent II, and wherein reagent I includes the component of following mass percent and mass concentration:
Ellagic acid 0.002%~0.005%,
Rabbit cephalin 0.03%~0.07%,
Stabilizer 5.0%~5.5%,
TRIS buffer 1.1g/L~1.3g/L,
Sodium chloride 0.8%~1.0%,
Surplus is water.
Preferably, the APTT detection reagent is made of reagent I and reagent II, wherein reagent I includes following quality hundred
Divide than the component with mass concentration:
Ellagic acid 0.003%,
Rabbit cephalin 0.05%,
Stabilizer 5.25%,
TRIS buffer 1.21g/L,
Sodium chloride 0.90%,
Surplus is water.
Preferably, the reagent II includes the component of following mass concentration and mass percent:
Calcium chloride 4.3g/L~4.6g/L,
Sodium azide 0.03%~0.05%,
Surplus is water.
Preferably, the reagent II includes the component of following mass concentration and mass percent:
Calcium chloride 4.44g/L,
Sodium azide 0.05%,
Surplus is water.
Preferably, the application method of the APTT detection reagent are as follows: reagent I, 37 DEG C of incubations are added in test plasma
After be added reagent II, record setting time, as activated partial thromboplastin time.
Preferably, the test plasma is the anticoagulant blood plasma of sodium citrate.
Preferably, the application method of the APTT detection reagent are as follows: take 50 μ L of test plasma, 37 DEG C of 50 μ L is added in advance
Then the reagent II of 50 37 DEG C of pre-temperatures of μ L is added in 37 DEG C of incubation 5min in the reagent I of temperature, record setting time, as activate
Partial thromboplastin time.
Compared with prior art, the invention has the following advantages: stabilizer is green non-poisonous, production process is safer
Environmental protection greatly reduces the production and safety in utilization of APTT detection reagent；Stablizing effect of the stabilizer to APTT detection reagent
It is good, it can be effectively reduced the deposited phenomenon of conventional APTT detection reagent and the phenomenon of long-term storing stability difference.
Specific embodiment
The following is specific embodiments of the present invention, and technical scheme of the present invention will be further described, but the present invention is simultaneously
It is not limited to these embodiments.
Embodiment 1
Stabilizer in the present embodiment for APTT detection reagent consists of the following mass percentage components:
Chitosan oligosaccharide 55.3%,
Glucosamine 20.8%,
Ascorbic acid 3.0%,
Butylated hydroxytoluene 1.2%,
Mannitol 19.7%.
Embodiment 2
Stabilizer in the present embodiment for APTT detection reagent consists of the following mass percentage components:
Chitosan oligosaccharide 57.14%,
Glucosamine 19.05%,
Ascorbic acid 3.81%,
Butylated hydroxytoluene 0.95%,
Mannitol 19.05%.
Embodiment 3
Stabilizer in the present embodiment for APTT detection reagent consists of the following mass percentage components:
Chitosan oligosaccharide 58.9%,
Glucosamine 17.2%,
Ascorbic acid 4.5%,
Butylated hydroxytoluene 0.7%,
Mannitol 18.7%.
Embodiment 4
APTT detection reagent in the present embodiment is made of reagent I and reagent II, and wherein reagent I is by following mass percent
It is grouped as with the group of mass concentration:
Ellagic acid 0.002%,
Rabbit cephalin 0.03%,
Stabilizer 5.0% in embodiment 2,
TRIS buffer 1.1g/L,
Sodium chloride 0.8%,
Surplus is water；
Reagent II includes the component of following mass concentration and mass percent:
Calcium chloride 4.3g/L,
Sodium azide 0.03%,
Surplus is water
Embodiment 5
APTT detection reagent in the present embodiment is made of reagent I and reagent II, and wherein reagent I is by following mass percent
It is grouped as with the group of mass concentration:
Ellagic acid 0.003%,
Rabbit cephalin 0.05%,
Stabilizer 5.25% in embodiment 2,
TRIS buffer 1.21g/L,
Sodium chloride 0.90%,
Surplus is water；
Reagent II includes the component of following mass concentration and mass percent:
Calcium chloride 4.44g/L,
Sodium azide 0.05%,
Surplus is water.
Embodiment 6
APTT detection reagent in the present embodiment is made of reagent I and reagent II, and wherein reagent I is by following mass percent
It is grouped as with the group of mass concentration:
Ellagic acid 0.005%,
Rabbit cephalin 0.07%,
Stabilizer 5.5% in embodiment 2,
TRIS buffer 1.3g/L,
Sodium chloride 1.0%,
Surplus is water.
Reagent II includes the component of following mass concentration and mass percent:
Calcium chloride 4.6g/L,
Sodium azide 0.05%,
Surplus is water
Embodiment 7
The present embodiment and the difference of embodiment 5 are, using the stabilizer in embodiment 1.
Embodiment 8
The present embodiment and the difference of embodiment 5 are, using the stabilizer in embodiment 3.
The application method of APTT detection reagent in embodiment 4~8 are as follows:
By in 4~8 any embodiment of embodiment reagent I and reagent II pre-temperature is carried out at a temperature of 37 DEG C, then
Reagent I after taking 50 μ L pre-temperatures is added in the anticoagulant blood plasma of sodium citrate to be measured, is incubated for 5min at a temperature of 37 DEG C after mixing,
Reagent II after adding 50 μ L pre-temperatures records setting time, as activated partial thromboplastin time.
Comparative example 1
Using 1wt% glycine and 0.1wt% Sodium azide substitution stabilizer in chitosan oligosaccharide and Glucosamine, other with
Embodiment 5 is identical.
Comparative example 2
It does not include chitosan oligosaccharide in stabilizer, other are same as Example 5.
Comparative example 3
It does not include Glucosamine in stabilizer, other are same as Example 5.
Comparative example 4
It does not include ascorbic acid in stabilizer, other are same as Example 5.
Comparative example 5
It does not include butylated hydroxytoluene in stabilizer, other are same as Example 5.
APTT detection reagent kit obtained in the embodiment of the present invention 4~8, comparative example 1~5 is protected from light at 2-8 DEG C
It saves, from 12 months on earth 0th month, monthly carries out APTT according to the application method of aforementioned APTT detection reagent with test plasma
Measurement, observe reagent stability, measurement result is as shown in table 1 below, and calculates the detection of each testing result Yu 0th month
As a result between deviation (calculation formula are as follows: | n-th month measured value-the, 0 month measured value |/the 0 month measured value, n=0,1,
2 ..., 12), the results are shown in Table 2.
Table 1: using APTT detection reagent obtained in embodiment 4~8, comparative example 1~5 to the testing result of test plasma
Table 2: the deviation in table 1 between each testing result and 0th month testing result
In comparing embodiment 4~8, comparative example 1~5 detection reagent stability test as a result, it has been found that, in embodiment 4~8
Test result deviation of the preparation from 0th month to 12nd month in the reasonable scope.And pair of inventive formulation is not used
Ratio 1 alreadyd exceed 30% from 7th month deviation, and deviation gradually greatly improved since 7th month, by 12nd month
When deviation be up to 96.4%, completely outstripped zone of reasonableness (inspection of the conventional APTT detection reagent to APTT of normal deviate
It surveys result and is usually no more than 35s).Reagent stability since 7th month or 8th month declines to a great extent in comparative example 2~5.
To obtain, kit containment of the invention can at least be stablized 12 months under 2~8 DEG C of light protected environments, and comparative example 1 may only
Stablize 6 months, APTT detection reagent formula of the present invention is changed in part to have certain reduction to the stability of detection reagent.
0~90 day deposited phenomenon is being placed to the reagent I of detection reagent in embodiment 4~8, comparative example 1~5 simultaneously
It is observed, as a result as shown in table 3 below.
Table 3: the observation result of the reagent I deposited phenomenon of APTT obtained in embodiment 4~8, comparative example 1~5 is used
Note: in table 3 -- ,+and ++ it respectively represents and precipitates on a small quantity, has more precipitating without precipitating, have
As shown in Table 3, APTT detection reagent made from the embodiment of the present invention 4~8 place 90 days in do not generate precipitating or
A small amount of precipitating is only generated, is had good stability.
Specific embodiment described herein is only an example for the spirit of the invention.The neck of technology belonging to the present invention
The technical staff in domain can make various modifications or additions to the described embodiments or replace by a similar method
In generation, however, it does not deviate from the spirit of the invention or beyond the scope of the appended claims.
Claims (7)
1. a kind of stabilizer for APTT detection reagent, which is characterized in that the stabilizer includes following mass percent
Component:
Chitosan oligosaccharide 55.3%~58.9%,
Glucosamine 17.2%~20.8%,
Surplus is auxiliary reagent.
2. stabilizer according to claim 1, which is characterized in that the auxiliary reagent includes accounting for stabilizer mass percent
3.0%~4.5% ascorbic acid.
3. stabilizer according to claim 1, which is characterized in that the auxiliary reagent includes accounting for stabilizer mass percent
0.7%~1.2% butylated hydroxytoluene.
4. stabilizer according to claim 1, which is characterized in that the auxiliary reagent includes accounting for stabilizer mass percent
18.7%~19.7% mannitol.
5. a kind of APTT detection reagent using the stabilizer as described in Claims 1 to 4 any claim, which is characterized in that
The APTT detection reagent is made of reagent I and reagent II, and wherein reagent I includes the group of following mass percent and mass concentration
Point:
Ellagic acid 0.002%~0.005%,
Rabbit cephalin 0.03%~0.07%,
Stabilizer 5.0%~5.5%,
TRIS buffer 1.1g/L~1.3g/L,
Sodium chloride 0.8%~1.0%,
Surplus is water.
6. APTT detection reagent according to claim 5, which is characterized in that the reagent II include following mass concentration and
The component of mass percent:
Calcium chloride 4.3g/L~4.6g/L,
Sodium azide 0.03%~0.05%,
Surplus is water.
7. APTT detection reagent according to claim 6, which is characterized in that the application method of the APTT detection reagent
Are as follows: reagent I is added in test plasma, reagent II is added after 37 DEG C of incubations, records setting time, as activated partial blood coagulation is living
The enzyme time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811249754.5A CN109444438B (en) | 2018-10-25 | 2018-10-25 | Stabilizer for APTT detection reagent and APTT detection reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811249754.5A CN109444438B (en) | 2018-10-25 | 2018-10-25 | Stabilizer for APTT detection reagent and APTT detection reagent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109444438A true CN109444438A (en) | 2019-03-08 |
| CN109444438B CN109444438B (en) | 2022-03-15 |
Family
ID=65548433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811249754.5A Active CN109444438B (en) | 2018-10-25 | 2018-10-25 | Stabilizer for APTT detection reagent and APTT detection reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109444438B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111929449A (en) * | 2020-08-28 | 2020-11-13 | 保定天岳生物工程有限公司 | APTT detection kit |
| CN112433057A (en) * | 2020-11-10 | 2021-03-02 | 北京美创新跃医疗器械有限公司 | Screening reagent for lupus anticoagulant and preparation method thereof |
| CN113009161A (en) * | 2021-02-09 | 2021-06-22 | 桂林优利特医疗电子有限公司 | Detection kit for activated partial thromboplastin time and preparation method thereof |
| CN113025684A (en) * | 2019-12-25 | 2021-06-25 | 深圳市帝迈生物技术有限公司 | Activated partial thromboplastin time detection reagent and kit |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050267222A1 (en) * | 2002-10-22 | 2005-12-01 | Dainippon Pharmaceutical Co. Ltd. | Stabilized composition |
| CN101561432A (en) * | 2009-05-27 | 2009-10-21 | 福建省洪诚生物药业有限公司 | Dilution being capable of maintaining high stability of enzyme marker solution |
| CN101892213A (en) * | 2010-07-13 | 2010-11-24 | 湖北大学 | Novel environmentally protected phytase heat stabilizer |
| US20170059594A1 (en) * | 2015-08-31 | 2017-03-02 | Sysmex Corporation | Reagent for activated partial thromboplastin time measurement, reagent kit for activated partial thromboplastin time measurement, and method for measuring activated partial thrombopalstin time |
| US20180139988A1 (en) * | 2015-06-12 | 2018-05-24 | Dainichiseika Color & Chemicals Mfg. Co., Ltd. | Preservative composition and preservation liquid for fruits and vegetables, and fruits and vegetables |
| CN108226539A (en) * | 2018-01-12 | 2018-06-29 | 三诺生物传感股份有限公司 | A kind of activated partial thromboplastin time detection reagent and detection method |
-
2018
- 2018-10-25 CN CN201811249754.5A patent/CN109444438B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050267222A1 (en) * | 2002-10-22 | 2005-12-01 | Dainippon Pharmaceutical Co. Ltd. | Stabilized composition |
| CN101561432A (en) * | 2009-05-27 | 2009-10-21 | 福建省洪诚生物药业有限公司 | Dilution being capable of maintaining high stability of enzyme marker solution |
| CN101892213A (en) * | 2010-07-13 | 2010-11-24 | 湖北大学 | Novel environmentally protected phytase heat stabilizer |
| US20180139988A1 (en) * | 2015-06-12 | 2018-05-24 | Dainichiseika Color & Chemicals Mfg. Co., Ltd. | Preservative composition and preservation liquid for fruits and vegetables, and fruits and vegetables |
| US20170059594A1 (en) * | 2015-08-31 | 2017-03-02 | Sysmex Corporation | Reagent for activated partial thromboplastin time measurement, reagent kit for activated partial thromboplastin time measurement, and method for measuring activated partial thrombopalstin time |
| CN108226539A (en) * | 2018-01-12 | 2018-06-29 | 三诺生物传感股份有限公司 | A kind of activated partial thromboplastin time detection reagent and detection method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113025684A (en) * | 2019-12-25 | 2021-06-25 | 深圳市帝迈生物技术有限公司 | Activated partial thromboplastin time detection reagent and kit |
| CN111929449A (en) * | 2020-08-28 | 2020-11-13 | 保定天岳生物工程有限公司 | APTT detection kit |
| CN112433057A (en) * | 2020-11-10 | 2021-03-02 | 北京美创新跃医疗器械有限公司 | Screening reagent for lupus anticoagulant and preparation method thereof |
| CN113009161A (en) * | 2021-02-09 | 2021-06-22 | 桂林优利特医疗电子有限公司 | Detection kit for activated partial thromboplastin time and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109444438B (en) | 2022-03-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109444438A (en) | A kind of stabilizer and APTT detection reagent for APTT detection reagent | |
| Shanmugam et al. | Heparinoid-active sulphated polysaccharides from marine algae as potential blood anticoagulant agents | |
| Yamada et al. | Preparation and anti-HIV activity of low-molecular-weight carrageenans and their sulfated derivatives | |
| Johnson et al. | Four heparin preparations: anti-Xa potentiating effect of heparin after subcutaneous injection | |
| Jouault et al. | Characterization, chemical modifications and in vitro anticoagulant properties of an exopolysaccharide produced by Alteromonas infernus | |
| Thimon et al. | Interactions of surfactin, a biosurfactant from Bacillus subtilis, with inorganic cations | |
| JPH04506089A (en) | Sulfated polysaccharides, anticoagulants, anticomplement agents obtained from brown algae and their production method | |
| Suzuki et al. | Influence of physico-chemical properties of chitin and chitosan on complement activation | |
| US20220202939A1 (en) | Preparation for improving activity and/or thermal stability of superoxide dismutase, and application thereof | |
| Cavalcante et al. | 2, 3-Di-O-sulfo glucuronic acid: An unmodified and unusual residue in a highly sulfated chondroitin sulfate from Litopenaeus vannamei | |
| Muzzarelli et al. | The blood anticoagulant activity of N-carboxymethylchitosan trisulfate | |
| JP6157464B2 (en) | Absorption enhancers as additives to improve oral formulations of non-anticoagulant sulfated polysaccharides | |
| EP3398971A1 (en) | Sulfated heparin oligosaccharide and preparation method and application thereof | |
| Michalski et al. | Neutralization of heparin in plasma by platelet factor 4 and protamine sulphate | |
| US20160009828A1 (en) | Preparation method for hyaluronic acid, and anti-adhesive composition comprising hyaluronic acid prepared by same preparation method | |
| EP1752772A1 (en) | Assay for determination of heparin based on factor Xa using a heparin-modifying component | |
| CN116640756A (en) | Heparinase I protective agent and application thereof in thromboelastography heparin detection kit | |
| Gao et al. | Sodium alginate/heparin composites on PVC surfaces inhibit the thrombosis and platelet adhesion: applications in cardiac surgery | |
| US20140371171A1 (en) | Aqueous Lock Composition Comprising Ethanol and a Polysaccharide Anticoagulant | |
| EP0123883A2 (en) | Method for the photometric determination of the activated partial thromboplastin time, and reagent therefor | |
| JP3939771B2 (en) | Additives for diagnostic tests for measuring blood coagulation | |
| CN103491967A (en) | Artificial saliva comprising hyaluronic acid | |
| US20020009782A1 (en) | Heparin and heparan sulfate derived oligosaccharides and a method for their manufacture | |
| Fouad | Chitosan as an antimicrobial compound: modes of action and resistance mechanisms | |
| CN112089693B (en) | Penicillin composition for injection and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |